##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4832134 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.076424412071354 33.0 31.0 34.0 30.0 34.0 2 32.263610446233486 34.0 31.0 34.0 30.0 34.0 3 32.34366679400861 34.0 31.0 34.0 30.0 34.0 4 35.95405528902965 37.0 35.0 37.0 35.0 37.0 5 35.863903815581274 37.0 35.0 37.0 35.0 37.0 6 35.90894002525592 37.0 35.0 37.0 35.0 37.0 7 35.831715345642316 37.0 35.0 37.0 35.0 37.0 8 35.820322242719264 37.0 35.0 37.0 33.0 37.0 9 37.45813878505853 39.0 37.0 39.0 34.0 39.0 10-11 37.383646335138884 39.0 37.0 39.0 34.0 39.0 12-13 37.30552122519781 39.0 37.0 39.0 34.0 39.0 14-15 38.487853813656656 40.0 38.0 41.0 34.0 41.0 16-17 38.437131813811455 40.0 38.0 41.0 33.5 41.0 18-19 38.60801345740826 40.0 38.0 41.0 34.0 41.0 20-21 38.61335167857514 40.0 38.0 41.0 34.0 41.0 22-23 38.53793365415777 40.0 38.0 41.0 34.0 41.0 24-25 38.44581565825782 40.0 38.0 41.0 34.0 41.0 26-27 38.26517673143998 40.0 38.0 41.0 33.5 41.0 28-29 38.10065314827776 40.0 37.5 41.0 33.0 41.0 30-31 37.882994759665195 40.0 37.0 41.0 33.0 41.0 32-33 37.71670404835628 40.0 37.0 41.0 33.0 41.0 34-35 37.65992209653126 40.0 37.0 41.0 33.0 41.0 36-37 37.52948479905565 40.0 36.5 41.0 32.5 41.0 38-39 37.40076816992244 40.0 36.0 41.0 32.0 41.0 40-41 37.23589650038679 40.0 36.0 41.0 31.5 41.0 42-43 37.01654672656015 40.0 36.0 41.0 31.0 41.0 44-45 36.74357550928844 39.5 35.5 41.0 30.5 41.0 46-47 36.39229830960814 39.0 35.0 41.0 30.0 41.0 48-49 36.046872561895015 39.0 35.0 41.0 29.0 41.0 50-51 35.79679547380101 39.0 35.0 41.0 28.0 41.0 52-53 35.576431034404266 39.0 35.0 40.0 28.0 41.0 54-55 35.3051343360925 38.5 35.0 40.0 27.0 41.0 56-57 35.02210503682224 38.0 34.0 40.0 26.5 41.0 58-59 34.72372382471181 38.0 34.0 40.0 26.0 41.0 60-61 34.33028616342179 37.5 34.0 40.0 26.0 41.0 62-63 33.99469344186233 37.0 33.0 40.0 25.0 41.0 64-65 33.604626651496005 36.0 33.0 39.0 24.0 41.0 66-67 33.18398517094104 36.0 32.5 39.0 23.0 40.0 68-69 32.84480521442493 35.5 32.0 39.0 22.5 40.0 70-71 32.39589330511116 35.0 32.0 38.0 21.5 40.0 72-73 31.910278771242687 35.0 31.0 37.0 20.5 39.5 74-75 31.431840155922828 35.0 31.0 37.0 18.5 39.0 76-77 29.501078819420158 32.5 28.5 35.0 14.5 37.0 78-79 30.453367083777067 34.0 30.0 36.0 14.5 37.5 80-81 30.496950415696254 34.0 31.0 35.5 14.5 37.0 82-83 30.181190546454218 34.0 30.0 35.0 10.0 37.0 84-85 29.788949975311116 34.0 30.0 35.0 7.0 36.0 86-87 29.23439064396807 34.0 29.0 35.0 2.0 36.0 88-89 28.63735101303068 34.0 29.0 35.0 2.0 35.5 90-91 28.12087092369541 33.5 27.5 35.0 2.0 35.0 92-93 27.843639476885368 33.0 27.0 35.0 2.0 35.0 94-95 27.503999785601973 33.0 27.0 35.0 2.0 35.0 96-97 26.880841984100606 33.0 25.5 35.0 2.0 35.0 98-99 26.066773603546594 33.0 24.5 35.0 2.0 35.0 100-101 22.37794554124534 28.5 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15323918015017313 1101 2 0.18324151351640694 1101 3 0.030030810749501313 1101 4 0.08018755646433817 1101 5 -0.04115816671174599 1101 6 0.0756024734235794 1101 7 0.21336495493390117 1101 8 0.1841560605875472 1101 9 0.19881148334484067 1101 10-11 0.26799474970245285 1101 12-13 0.15974467615702537 1101 14-15 0.25635391548295416 1101 16-17 0.2811251328567579 1101 18-19 0.2838311362803836 1101 20-21 0.2844504161878021 1101 22-23 0.22628637841952326 1101 24-25 0.24885888419213842 1101 26-27 0.06002824392797379 1101 28-29 0.12387674624199718 1101 30-31 0.23871281806572853 1101 32-33 0.368652231137375 1101 34-35 0.43100560589144266 1101 36-37 0.4287492184295232 1101 38-39 0.4594440961815849 1101 40-41 0.4383759246684278 1101 42-43 0.4630651996011963 1101 44-45 0.4365298733619838 1101 46-47 0.44516222516117665 1101 48-49 0.3740276004918073 1101 50-51 0.31751725337429804 1101 52-53 0.19683445731838134 1101 54-55 0.32219310932175915 1101 56-57 0.3010062476272495 1101 58-59 0.3349955736892767 1101 60-61 0.396204221741435 1101 62-63 0.3085706888061708 1101 64-65 0.2814874391730129 1101 66-67 0.2887476248226406 1101 68-69 0.24144096970497486 1101 70-71 0.3042518249169177 1101 72-73 0.20401292685908956 1101 74-75 0.24631187851347391 1101 76-77 0.2089251409719246 1101 78-79 0.02293757506021521 1101 80-81 0.19647373779851662 1101 82-83 0.26109922855329515 1101 84-85 0.25430062333751735 1101 86-87 0.393342277250877 1101 88-89 0.5278192231077874 1101 90-91 0.4661377668028308 1101 92-93 0.518109029599163 1101 94-95 0.4978601461637133 1101 96-97 0.436270852661373 1101 98-99 0.27986746267098184 1101 100-101 0.6810095174294482 1102 1 0.25032442786181974 1102 2 0.1696206227517223 1102 3 0.20289133715902352 1102 4 0.1251782071748906 1102 5 -0.02800567534870879 1102 6 0.001256923685360789 1102 7 0.17223569432913877 1102 8 0.15518363653939105 1102 9 0.26147091099024067 1102 10-11 0.235342111422014 1102 12-13 0.26630596297541587 1102 14-15 0.29082454535794255 1102 16-17 0.36096450315984896 1102 18-19 0.3005439038481441 1102 20-21 0.32746527265394576 1102 22-23 0.2131569041167154 1102 24-25 0.23694243567556583 1102 26-27 0.2167468881919703 1102 28-29 0.27530596321674494 1102 30-31 0.25871292936679424 1102 32-33 0.20125925824140722 1102 34-35 0.19837269015970804 1102 36-37 0.1397755923298618 1102 38-39 0.302047984595589 1102 40-41 0.3044466186528254 1102 42-43 0.31667856641389136 1102 44-45 0.2723376074503818 1102 46-47 0.3263238298554114 1102 48-49 0.29962867076284283 1102 50-51 0.11838350956264065 1102 52-53 0.05591243929651313 1102 54-55 0.09360131265544425 1102 56-57 -0.008813489167991406 1102 58-59 0.10347731586258391 1102 60-61 0.018243346291782814 1102 62-63 -7.114881893244274E-5 1102 64-65 0.05907307421236396 1102 66-67 0.23754541749992342 1102 68-69 0.11883630218352437 1102 70-71 0.13691267721595324 1102 72-73 0.07554400656846028 1102 74-75 0.07816029981916728 1102 76-77 0.07644245941763117 1102 78-79 0.07638931046642128 1102 80-81 0.19815008765783304 1102 82-83 0.25286606617839524 1102 84-85 0.35038563063886485 1102 86-87 0.36279098081606875 1102 88-89 0.3544415251699711 1102 90-91 0.48031271426872735 1102 92-93 0.5322059550185472 1102 94-95 0.5897831169226997 1102 96-97 0.6906407773328134 1102 98-99 0.7245185560926437 1102 100-101 1.455711163794799 1103 1 0.21639345144002675 1103 2 0.18133798966154302 1103 3 0.17881341273010065 1103 4 0.07178714881117543 1103 5 -0.013846059823585222 1103 6 0.07226983439004186 1103 7 0.09734732574100491 1103 8 0.09629202255408131 1103 9 0.178543641256951 1103 10-11 0.22086288534455178 1103 12-13 0.1715409979975746 1103 14-15 0.20386319023014465 1103 16-17 0.1735246046221306 1103 18-19 0.05396833222312125 1103 20-21 0.17559224445628985 1103 22-23 0.24757964109014097 1103 24-25 0.20423818885089418 1103 26-27 0.19877327667342826 1103 28-29 0.16203407723313745 1103 30-31 0.2303476346147164 1103 32-33 0.2099683295409136 1103 34-35 0.24613728108808175 1103 36-37 0.2825726317773629 1103 38-39 0.22483791383153573 1103 40-41 0.26865568940706197 1103 42-43 0.3259352330551124 1103 44-45 0.3879946422522451 1103 46-47 0.39830586675653734 1103 48-49 0.32996041883888694 1103 50-51 0.2783638147128542 1103 52-53 0.2587506787121612 1103 54-55 0.1845002418706727 1103 56-57 0.12323003143362854 1103 58-59 0.24049510556826448 1103 60-61 0.30592071717109803 1103 62-63 0.06664872084173368 1103 64-65 0.2250356627219645 1103 66-67 0.1807138455433872 1103 68-69 0.16905459222126495 1103 70-71 0.22615979087355242 1103 72-73 0.3099119556541048 1103 74-75 0.42158314693543986 1103 76-77 0.4565813920789097 1103 78-79 0.25622232954730606 1103 80-81 0.49485184372741386 1103 82-83 0.43922263892556757 1103 84-85 0.5344687023375023 1103 86-87 0.5789561994496246 1103 88-89 0.4269109169088203 1103 90-91 0.2790845451809183 1103 92-93 0.3882383261105211 1103 94-95 0.5349721972953496 1103 96-97 0.6556391470005636 1103 98-99 0.8277529389818987 1103 100-101 1.57190940347218 1104 1 0.07306505752098502 1104 2 0.05315777472287664 1104 3 0.0035942427448176772 1104 4 0.01340065958638803 1104 5 -0.09076931911972963 1104 6 0.01827631448318101 1104 7 0.020914961691573808 1104 8 -0.04275241803568974 1104 9 0.04831958128600178 1104 10-11 0.11040307064672561 1104 12-13 -0.026928213197386697 1104 14-15 -0.010612059648813954 1104 16-17 0.022406330766386873 1104 18-19 0.04386064477252205 1104 20-21 0.08985767843044812 1104 22-23 0.051243720627930145 1104 24-25 0.12092704092503226 1104 26-27 0.015352302359303849 1104 28-29 0.04566586751965218 1104 30-31 -0.06996417094179463 1104 32-33 -0.07949884220587222 1104 34-35 -0.06980249880739109 1104 36-37 0.007758346915359482 1104 38-39 -0.012948006354790209 1104 40-41 0.010879387974277677 1104 42-43 0.036162070800010326 1104 44-45 0.0806575989555327 1104 46-47 -0.0031591977788565373 1104 48-49 0.16983685962592432 1104 50-51 0.04365915881196969 1104 52-53 0.01749152809370713 1104 54-55 0.05100570223972056 1104 56-57 0.012332665611666016 1104 58-59 0.16657332068211161 1104 60-61 0.33589272131344927 1104 62-63 0.18391349977161298 1104 64-65 0.05091292459897545 1104 66-67 0.06495119399865956 1104 68-69 0.09580543900263194 1104 70-71 0.07185837084654878 1104 72-73 0.10965896010682386 1104 74-75 0.1588528468875765 1104 76-77 0.09664575073901105 1104 78-79 0.005516161130405095 1104 80-81 0.13986487736692865 1104 82-83 0.2165718149388418 1104 84-85 0.13851780358710286 1104 86-87 0.22309826905156882 1104 88-89 0.29447476777598425 1104 90-91 0.24865201422365146 1104 92-93 0.3741073063933271 1104 94-95 0.3483924534574889 1104 96-97 0.32173045868921335 1104 98-99 0.5469826696809399 1104 100-101 1.1036588337784998 1105 1 0.10259024900216218 1105 2 0.05983356399405437 1105 3 0.11247640750747223 1105 4 0.018800700935365455 1105 5 -0.12204194749862296 1105 6 -0.08013688505937466 1105 7 0.011902145178162016 1105 8 0.050650557821583675 1105 9 0.09081592734555954 1105 10-11 0.08694522806810312 1105 12-13 0.06885464604774683 1105 14-15 0.10724369771217113 1105 16-17 0.0972379205177134 1105 18-19 0.13377665841701258 1105 20-21 0.05129080046537382 1105 22-23 -0.0014194683936850083 1105 24-25 0.040434139723039664 1105 26-27 0.07774362769092846 1105 28-29 0.10247395348277877 1105 30-31 0.11552703855925017 1105 32-33 0.1588315316249691 1105 34-35 0.179858388632546 1105 36-37 0.1680144675995976 1105 38-39 0.24358543705321978 1105 40-41 0.2200557049626326 1105 42-43 0.2997507463234186 1105 44-45 0.2509307017788842 1105 46-47 0.1608205646014511 1105 48-49 0.15929360067844556 1105 50-51 0.1809128852300219 1105 52-53 0.2061615238318879 1105 54-55 0.24779544502101203 1105 56-57 0.2614279062039344 1105 58-59 0.18049100531966644 1105 60-61 0.20796967333260596 1105 62-63 0.24922371898132667 1105 64-65 0.20190234837857446 1105 66-67 0.19337784865920327 1105 68-69 0.23789744265122437 1105 70-71 0.15836269712539774 1105 72-73 0.26908922846103067 1105 74-75 0.2561735382772383 1105 76-77 0.32756796071834415 1105 78-79 0.10214042449734961 1105 80-81 0.1972939077567588 1105 82-83 0.2601053924471337 1105 84-85 0.34392603624224094 1105 86-87 0.37301178021445835 1105 88-89 0.2596218455588222 1105 90-91 0.1425731382077977 1105 92-93 0.20452386812888435 1105 94-95 0.4667186473007021 1105 96-97 0.4828941986408779 1105 98-99 0.5517120008695571 1105 100-101 1.3693835948695785 1106 1 0.16348486553649622 1106 2 0.15464705067159912 1106 3 0.10761470827032582 1106 4 0.06299100003747782 1106 5 0.022458031557533786 1106 6 0.059293801983642425 1106 7 0.09541679118152757 1106 8 0.08528372525723427 1106 9 0.11087687632780785 1106 10-11 0.10532990751259774 1106 12-13 0.1257924616196604 1106 14-15 0.09305994335949919 1106 16-17 0.1500002485354841 1106 18-19 -0.01072267868235599 1106 20-21 -0.012561571781610326 1106 22-23 0.07355544714486228 1106 24-25 0.0635839239693965 1106 26-27 0.11027619215261808 1106 28-29 0.18274326719405565 1106 30-31 0.24286329985748978 1106 32-33 0.2029038912286154 1106 34-35 0.23167009901566615 1106 36-37 0.24302701785477865 1106 38-39 0.3273681601986027 1106 40-41 0.4899420015765159 1106 42-43 0.41204174726083664 1106 44-45 0.46486456642775664 1106 46-47 0.47988081128298177 1106 48-49 0.4792003676893799 1106 50-51 0.41626198694791583 1106 52-53 0.4533642171936876 1106 54-55 0.4868082543057639 1106 56-57 0.49122520513865453 1106 58-59 0.4650912724699836 1106 60-61 0.45112827967009395 1106 62-63 0.48389930772390954 1106 64-65 0.5361549237085939 1106 66-67 0.4599412365155331 1106 68-69 0.39607451166592256 1106 70-71 0.400668524054808 1106 72-73 0.5039620383652696 1106 74-75 0.6111608162986073 1106 76-77 0.6279799645720097 1106 78-79 0.46314055064185666 1106 80-81 0.4267979528067194 1106 82-83 0.5722090543451834 1106 84-85 0.5371325807028029 1106 86-87 0.6752315544414635 1106 88-89 0.7289264410546288 1106 90-91 0.5801543758330752 1106 92-93 0.7710667013313426 1106 94-95 0.9380080728761655 1106 96-97 1.0440131590111186 1106 98-99 1.2718607695402717 1106 100-101 1.824301216567335 1107 1 0.03236671010741787 1107 2 0.018332346693462398 1107 3 0.0107821404463877 1107 4 0.004781615022643848 1107 5 -0.04691027778390122 1107 6 -0.08700240072744236 1107 7 -0.007706239224944511 1107 8 -0.001963368206745031 1107 9 -0.020989466399825574 1107 10-11 -0.0847192808758166 1107 12-13 0.011628622903927521 1107 14-15 0.05149375278023882 1107 16-17 -0.013710180413575301 1107 18-19 0.030272439030007092 1107 20-21 -0.03707340658541369 1107 22-23 0.018332097026579675 1107 24-25 0.021704386256338637 1107 26-27 -0.07861630947868292 1107 28-29 0.01588240237980898 1107 30-31 0.028957933790806578 1107 32-33 0.02829858068797364 1107 34-35 0.0557508520722223 1107 36-37 0.04753507676007018 1107 38-39 -0.05381622442688183 1107 40-41 -0.230766894764578 1107 42-43 -0.13426545259575562 1107 44-45 -0.022694992173526884 1107 46-47 -0.08717758101698792 1107 48-49 -0.02293541463833293 1107 50-51 -0.11865037438395376 1107 52-53 -0.11519841615861992 1107 54-55 -0.024770254029974126 1107 56-57 -0.1026827099773655 1107 58-59 -0.04395364133432622 1107 60-61 -0.0021543126431495807 1107 62-63 -0.028042169980970755 1107 64-65 -0.09660430141855159 1107 66-67 0.008766297993069827 1107 68-69 -0.03810121572691827 1107 70-71 -0.13157424842092524 1107 72-73 -0.1292423963449032 1107 74-75 -0.07065278939523978 1107 76-77 -0.2256537894815942 1107 78-79 0.020663051087620232 1107 80-81 0.09983303147603806 1107 82-83 0.020573403760732134 1107 84-85 0.08860617436335971 1107 86-87 0.06967294235750998 1107 88-89 0.041995706226003904 1107 90-91 0.330601770542561 1107 92-93 0.3913821477757189 1107 94-95 0.43777315191735156 1107 96-97 0.6195077286143125 1107 98-99 0.9074231543443751 1107 100-101 1.3638056807409527 1108 1 0.10643585934427335 1108 2 0.04462339212766864 1108 3 0.04608361846379694 1108 4 0.003981791180429184 1108 5 -0.022935825533224374 1108 6 0.037509308425754284 1108 7 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Count 2 3.0 3 23.0 4 53.0 5 155.0 6 372.0 7 882.0 8 1947.0 9 3413.0 10 5794.0 11 8594.0 12 12279.0 13 16932.0 14 20572.0 15 27687.0 16 36151.0 17 48888.0 18 45797.0 19 30855.0 20 28488.0 21 30664.0 22 31750.0 23 34738.0 24 40538.0 25 48157.0 26 57681.0 27 70300.0 28 85441.0 29 104789.0 30 125916.0 31 153588.0 32 188603.0 33 239307.0 34 320762.0 35 452480.0 36 672601.0 37 1003981.0 38 810037.0 39 71913.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64144630938196 19.548895420042417 14.822629949118957 21.987028321456663 2 15.718686609270355 21.731495856696025 38.5775518642488 23.97226566978482 3 20.33668768291608 25.294414434699036 29.90910434189118 24.459793540493706 4 12.863115964913224 17.15813344580262 37.26746816209981 32.711282427184344 5 13.305920072071595 38.42176124959427 33.51842528865071 14.753893389683428 6 30.922905022383308 36.66737790485785 17.187288193646083 15.222428879112757 7 28.199838828972872 33.71783563949178 20.367398751773024 17.714926779762315 8 26.409263484828855 33.55366386776525 19.507116317552452 20.529956329853434 9 26.44040914428284 17.626270297967732 20.8121297960694 35.12119076168004 10-11 25.365676945217164 26.459251750882736 26.630242456024604 21.544828847875493 12-13 27.200208024032445 24.115318407974613 26.97672498320618 21.707748584786764 14-15 23.214370710746017 25.471623924336534 26.920693838374515 24.39331152654293 16-17 21.84288651558314 28.940942490508558 27.121193463969778 22.094977529938525 18-19 21.48012658589352 27.65817338674797 29.547307669861805 21.314392357496708 20-21 22.9713621352388 25.936666905346584 29.134467297471467 21.95750366194315 22-23 22.17560605728235 26.086755872250233 29.335599964736076 22.40203810573134 24-25 22.698477706562155 26.30918291092703 29.39996072114976 21.592378661361057 26-27 22.364771093772312 26.0277591407071 30.156024358938804 21.451445406581783 28-29 21.33340052241929 27.280327904813902 29.86512791242958 21.521143660337234 30-31 21.767494444483535 26.290816852347227 29.61925310846098 22.32243559470826 32-33 21.799912833543107 26.59411969949509 29.58509635701328 22.020871109948523 34-35 22.900675597295706 26.171486662781984 29.20120737650794 21.72663036341437 36-37 22.703335404906262 25.82120962955412 29.72937254108519 21.74608242445443 38-39 22.040019041902532 26.529157913255858 29.757143300665433 21.67367974417618 40-41 22.03048493510977 26.531873473880196 29.6487694388184 21.788872152191633 42-43 21.770490622558782 26.922363290900968 28.634007477862216 22.673138608678034 44-45 22.125476011878035 26.40910432831013 28.626038363238308 22.83938129657352 46-47 22.711226653046662 27.011906604736485 28.195686023573973 22.08118071864288 48-49 22.748323925836452 26.84432830327415 28.176009335995143 22.231338434894255 50-51 21.84844437762326 27.0808225807503 28.71043401429203 22.360299027334406 52-53 22.49680989806988 27.635833360581476 27.62512380658318 22.242232934765468 54-55 21.736379827215057 26.800984823682455 28.114659071954545 23.347976277147943 56-57 22.485064622959015 27.095337554783743 27.65681563844048 22.762782183816764 58-59 23.236193065609516 26.539485058211522 27.626285693709118 22.598036182469844 60-61 22.411071843993525 26.891996892800908 28.740763796888018 21.95616746631755 62-63 22.04523896516532 26.901848276798333 28.529703107068144 22.523209650968198 64-65 21.646696025487312 27.48519960931708 28.08794566862543 22.780158696570183 66-67 22.064281170049004 27.09180055903868 27.466593651396792 23.377324619515523 68-69 21.681805285171755 27.044065310423598 28.08176654443606 23.19236285996859 70-71 22.413232916650454 27.053162041103178 27.65646716604596 22.8771378762004 72-73 22.25666755102404 26.522629546283277 28.238920940520273 22.981781962172406 74-75 21.648294259827047 27.944476849194572 27.42179633516245 22.985432555815933 76-77 22.051724443184522 26.98016483181413 27.545576407645488 23.422534317355858 78-79 22.101765243979525 27.01476307498712 27.784573005266843 23.098898675766517 80-81 22.383305255383107 27.432245324403027 27.40518660325303 22.77926281696084 82-83 22.278318255835647 27.01017292619002 27.714422981109315 22.99708583686502 84-85 21.845565541021834 27.139655067512614 27.525519780701448 23.489259610764105 86-87 22.011457039932868 27.09716420496962 27.520202295903545 23.37117645919397 88-89 21.904931417771056 27.197975623030402 27.0330090042116 23.86408395498694 90-91 22.015786021997876 26.722962159900298 27.510424510999304 23.750827307102526 92-93 22.32219677559646 26.99486777900832 27.47896186734659 23.203973578048632 94-95 21.965205951549834 26.70169891286836 27.517748681505083 23.815346454076725 96-97 21.423187823343365 26.64723907526514 28.053782068408328 23.87579103298317 98-99 21.7750997799316 27.04320699715695 26.916347932404193 24.26534529050726 100-101 21.687076113869068 26.08653092376858 27.840187116262243 24.386205846100104 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3829.0 1 2478.0 2 1372.5 3 1703.0 4 1522.0 5 1482.0 6 2667.5 7 5597.5 8 8542.0 9 9548.5 10 8150.5 11 6523.0 12 6412.0 13 6694.0 14 7153.0 15 7549.5 16 7850.5 17 8120.0 18 8413.5 19 8971.0 20 9740.0 21 10880.0 22 12609.5 23 14708.5 24 17311.0 25 20921.0 26 25984.0 27 31943.5 28 37117.0 29 43366.0 30 51590.5 31 60541.0 32 72140.5 33 82545.0 34 92059.5 35 100971.0 36 111459.5 37 122425.0 38 126903.0 39 127890.0 40 128707.0 41 135817.5 42 144332.5 43 148989.0 44 151848.5 45 155799.5 46 157584.5 47 183330.5 48 257748.5 49 257903.5 50 191622.5 51 163718.0 52 152104.5 53 150928.5 54 152548.0 55 144190.0 56 128871.5 57 115663.0 58 107050.0 59 96863.0 60 84821.5 61 73033.0 62 61387.5 63 49328.5 64 38769.5 65 30814.5 66 22116.5 67 17670.0 68 12576.0 69 6797.0 70 5075.0 71 4191.0 72 3461.5 73 2877.5 74 2377.0 75 2121.5 76 1438.5 77 713.5 78 428.5 79 277.0 80 164.5 81 113.0 82 81.0 83 46.0 84 24.5 85 15.0 86 11.5 87 6.0 88 2.5 89 3.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23753480346364567 2 0.0 3 0.0 4 0.0 5 0.02860020024279128 6 0.003828536211951076 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0030835237598957313 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.5181143569280155E-4 26-27 0.0014072457427712062 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0069431021573491125 36-37 0.01782856187349109 38-39 0.014155236589051545 40-41 0.009022928586003617 42-43 0.00839173748079006 44-45 0.003942357558792864 46-47 5.380645487066377E-4 48-49 0.0017280149929617017 50-51 1.034739516743534E-5 52-53 0.0 54-55 0.0 56-57 0.0013762035572689002 58-59 3.1042185502306024E-4 60-61 0.002679975348365753 62-63 7.967494278925211E-4 64-65 0.0017073202026268311 66-67 0.005546203809745342 68-69 2.2764269368357747E-4 70-71 4.138958066974136E-5 72-73 0.0 74-75 4.86327572869461E-4 76-77 0.06019079768897137 78-79 0.1418007033745339 80-81 0.0010450869119109695 82-83 9.002233795668746E-4 84-85 0.0 86-87 0.10648504366807708 88-89 0.35637463696164057 90-91 0.39482555740383024 92-93 3.2076925019049554E-4 94-95 3.000744598556249E-4 96-97 6.208437100461204E-5 98-99 0.0 100-101 0.0012002978394224995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4832134.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.683518176071342 #Duplication Level Percentage of deduplicated Percentage of total 1 63.82859774239169 20.223145367242136 2 15.363033340148219 9.735098921443523 3 7.019346373261703 6.671927652041329 4 3.8876025137789787 4.926916996266277 5 2.388528533119928 3.7838493596585137 6 1.6034630919324 3.0482011210739848 7 1.0832379181593355 2.4024551788307686 8 0.8303455262001688 2.1046614057426054 9 0.6089184785590376 1.7363411714853876 >10 2.9590432662023 16.514321677608013 >50 0.21997503625906678 4.875518957522917 >100 0.18301510729968037 11.54683900442714 >500 0.01683557827868681 3.6394359647893006 >1k 0.006878348885869489 4.112510651163422 >5k 6.550808462732828E-4 1.5286875993102913 >10k+ 5.240646770186262E-4 3.1500889713945233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38713 0.8011574182338487 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23450 0.4852928333527175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19839 0.4105639454534994 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18064 0.37383069260910395 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14375 0.29748761106376603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13471 0.27877952060104294 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12876 0.26646612035179484 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10985 0.22733227182855442 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9520 0.19701440398796885 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9176 0.18989539611277337 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8617 0.17832700831558065 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7941 0.16433733004920806 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7214 0.14929221747575708 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6975 0.144346162585723 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6724 0.13915177021167044 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6245 0.12923896564126738 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5972 0.1235892878798477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5269 0.10904085027443361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.069479033487068E-5 0.0 9 0.0 0.0 0.0 2.069479033487068E-5 0.0 10-11 0.0 0.0 0.0 6.208437100461204E-5 0.0 12-13 0.0 0.0 0.0 1.345161371766594E-4 0.0 14-15 0.0 0.0 0.0 2.1729529851614214E-4 0.0 16-17 0.0 0.0 0.0 3.5181143569280155E-4 0.0 18-19 0.0 0.0 0.0 5.070223632043316E-4 0.0 20-21 0.0 0.0 0.0 7.139702665530385E-4 0.0 22-23 0.0 0.0 0.0 0.0012520348152596761 0.0 24-25 0.0 0.0 0.0 0.002245384751333469 0.0 26-27 0.0 0.0 0.0 0.003342208639081615 0.0 28-29 0.0 0.0 0.0 0.007263871407539608 0.0 30-31 0.0 0.0 0.0 0.01790099363966314 0.0 32-33 0.0 0.0 0.0 0.03897863759572893 0.0 34-35 0.0 0.0 0.0 0.07116938396162027 0.0 36-37 0.0 0.0 0.0 0.12386866754936846 0.0 38-39 0.0 0.0 0.0 0.19644529725375992 0.0 40-41 0.0 0.0 0.0 0.27976252314194927 0.0 42-43 0.0 0.0 0.0 0.3658631983301788 0.0 44-45 0.0 0.0 0.0 0.4736623611845201 0.0 46-47 0.0 0.0 0.0 0.6056744287306601 0.0 48-49 0.0 0.0 0.0 0.7444640401114704 0.0 50-51 0.0 0.0 0.0 0.8957326100642077 0.0 52-53 0.0 0.0 0.0 1.047984182557851 0.0 54-55 0.0 0.0 0.0 1.2138943166725094 2.069479033487068E-5 56-57 0.0 0.0 0.0 1.4204179768193514 4.138958066974136E-5 58-59 0.0 0.0 0.0 1.6420902234913188 4.138958066974136E-5 60-61 0.0 0.0 0.0 1.8903449283484273 4.138958066974136E-5 62-63 0.0 0.0 0.0 2.134533106904734 6.208437100461204E-5 64-65 0.0 0.0 0.0 2.3811632707205552 6.208437100461204E-5 66-67 0.0 0.0 0.0 2.6710972833120934 6.208437100461204E-5 68-69 0.0 0.0 0.0 3.0007549459514165 6.208437100461204E-5 70-71 0.0 0.0 0.0 3.366266332845902 6.208437100461204E-5 72-73 0.0 0.0 0.0 3.7297185881020685 6.208437100461204E-5 74-75 0.0 0.0 0.0 4.107336427342453 6.208437100461204E-5 76-77 0.0 0.0 0.0 4.497609130872613 6.208437100461204E-5 78-79 0.0 1.034739516743534E-5 0.0 4.956660970080714 6.208437100461204E-5 80-81 0.0 2.069479033487068E-5 0.0 5.461065442307684 7.243176617204738E-5 82-83 0.0 2.069479033487068E-5 0.0 5.95314616689024 8.277916133948272E-5 84-85 0.0 2.069479033487068E-5 0.0 6.466418356775702 8.277916133948272E-5 86-87 0.0 2.069479033487068E-5 0.0 6.9886824330616655 8.277916133948272E-5 88-89 0.0 2.069479033487068E-5 0.0 7.550421821911396 8.277916133948272E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 26755 0.0 24.646597 1 TACATGG 27270 0.0 23.774721 2 ACATGGG 27260 0.0 22.738783 3 GAGTACT 20810 0.0 21.385029 12-13 AGTACTT 21050 0.0 20.79186 12-13 GTACTTT 21805 0.0 20.409193 14-15 CATGGGG 9400 0.0 20.196775 4 CATGGGA 17365 0.0 19.35122 4 GTAAGCG 375 0.0 18.977505 94-95 TGCACCG 1575 0.0 18.38227 5 GTGTAAG 1870 0.0 18.064062 1 GCACCGT 1790 0.0 18.023327 6 ACTTTTT 24880 0.0 17.934057 16-17 TACTTTT 24615 0.0 17.636011 14-15 ATGGGCG 700 0.0 17.628899 5 GAGTGCA 3140 0.0 17.073002 9 GATGTGC 2910 0.0 16.955145 8 ATGGGAT 6680 0.0 16.910263 5 GTGTAGC 2150 0.0 16.59669 1 TCGCGCT 1380 0.0 16.503117 60-61 >>END_MODULE