FastQCFastQC Report
Thu 26 May 2016
SRR938817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523144
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212160.3841290395470406No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT177260.3209403919217026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146600.26542853128580385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103470.187338950423889No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT99560.1802596492142881No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT76350.1382364826989845No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC69530.12588844324899007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG259750.026.0814171
TACATGG254700.025.8785572
ACATGGG251750.025.0901093
GTATCAA432750.023.2358261
GAGTACT200200.021.67622212-13
CATGGGG120350.021.4187764
AGTACTT205950.021.26645712-13
AGAGTAC331300.021.03071610-11
GTACTTT212050.020.19785114-15
CAACGCA500200.019.931685
TATCAAC503450.019.8971332
TCAACGC501600.019.8854964
ATCAACG501200.019.8824583
AACGCAG509800.019.5689756
GGTATCA329000.018.9200941
CATGGGA125900.018.8185484
TAAGGTG24550.018.3363955
GAGTACA206600.018.1280571
ACGCAGA552450.017.9469687
CGCAGAG552900.017.8551258
Produced by FastQC (version 0.11.5)