FastQCFastQC Report
Thu 26 May 2016
SRR938817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523144
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201280.3644301144420642No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT189010.3422145068098894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155550.28163306986021003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.18996426672923974No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90910.16459827953064415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT72510.1312839208972281No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC64710.11716152973741042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG253150.024.7990861
TACATGG248250.024.7336832
ACATGGG250000.023.554593
GTATCAA460750.021.7096161
AGAGTAC346850.020.94513510-11
GAGTACT220300.020.76424412-13
AGTACTT226850.020.52030612-13
CATGGGG120950.020.0866664
GTACTTT227050.019.74994314-15
CATGGGA121550.018.7382934
CAACGCA533400.018.6458445
TCAACGC534050.018.6142644
ATCAACG534200.018.6090373
TATCAAC537300.018.5458282
AACGCAG545200.018.28586
GGTATCA358250.018.1353681
GTATTAG14850.017.962211
TACTTTT262350.017.418114-15
TAAGGTG24350.017.3434225
GGACCGT5850.017.0336286
Produced by FastQC (version 0.11.5)