FastQCFastQC Report
Thu 26 May 2016
SRR938816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5529278
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211100.38178583171256714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT177390.3208194632282913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145120.2624574130655033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101320.18324273078691286No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT97190.17577340115653436No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT76600.13853526626803717No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC69860.12634560967996183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG256250.026.220991
TACATGG253200.025.8543742
ACATGGG252300.024.8563163
GTATCAA436500.023.2857421
CATGGGG122700.021.5344224
AGAGTAC330800.021.31250610-11
GAGTACT203450.021.31187212-13
AGTACTT211600.021.05180412-13
CAACGCA504900.020.0498735
ATCAACG505450.020.0468273
TATCAAC506400.020.0277732
TCAACGC506800.020.0121464
GTACTTT217250.019.84932314-15
AACGCAG514650.019.669146
CATGGGA124200.019.211844
GGTATCA331950.018.552221
ACGCAGA553400.018.0348877
CGCAGAG554250.017.878858
TACTTTT251900.017.49582114-15
TAAGGTG25000.017.4570315
Produced by FastQC (version 0.11.5)