##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938816_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5529278 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56204119235821 33.0 31.0 34.0 30.0 34.0 2 31.6189737611312 33.0 31.0 34.0 30.0 34.0 3 31.635741230591048 33.0 31.0 34.0 28.0 34.0 4 35.19988450571666 37.0 35.0 37.0 33.0 37.0 5 35.233889668777735 37.0 35.0 37.0 33.0 37.0 6 35.295795762123014 37.0 35.0 37.0 33.0 37.0 7 35.201845340386214 37.0 35.0 37.0 32.0 37.0 8 35.1724773107809 37.0 35.0 37.0 32.0 37.0 9 36.73895271679232 39.0 37.0 39.0 32.0 39.0 10-11 36.62218448773963 39.0 37.0 39.0 32.0 39.0 12-13 36.51341639903076 39.0 37.0 39.0 32.0 39.0 14-15 37.656598836231424 40.0 37.0 41.0 32.0 41.0 16-17 37.55391996929798 40.0 37.0 41.0 32.0 41.0 18-19 37.517058285005746 40.0 37.0 41.0 32.0 41.0 20-21 37.4036803720124 40.0 37.0 41.0 31.5 41.0 22-23 37.40787178723877 40.0 37.0 41.0 32.0 41.0 24-25 37.33353043561927 40.0 37.0 41.0 31.0 41.0 26-27 37.198849470039306 40.0 37.0 41.0 31.0 41.0 28-29 37.03328987979985 40.0 36.5 41.0 30.5 41.0 30-31 36.804416055767135 39.5 36.0 41.0 30.0 41.0 32-33 36.60460995088328 39.0 36.0 41.0 30.0 41.0 34-35 36.30457665539696 39.0 35.0 41.0 30.0 41.0 36-37 36.08832554630098 39.0 35.0 41.0 29.0 41.0 38-39 35.911401452413855 39.0 35.0 41.0 28.0 41.0 40-41 35.79835351016896 39.0 35.0 40.5 27.0 41.0 42-43 35.7763698081377 39.0 35.0 41.0 27.5 41.0 44-45 35.5852894356189 39.0 35.0 41.0 26.5 41.0 46-47 35.276695257500165 39.0 35.0 41.0 25.5 41.0 48-49 34.97198757233765 39.0 34.5 41.0 24.0 41.0 50-51 33.81802569883446 38.0 33.0 39.5 23.0 40.5 52-53 33.882718774494606 38.0 33.5 39.5 23.0 40.5 54-55 34.375382283184166 38.0 34.0 40.0 23.0 41.0 56-57 34.25582499197906 38.0 34.0 40.0 23.0 41.0 58-59 34.04411588637794 38.0 34.0 40.0 22.0 41.0 60-61 33.727169804086536 37.5 33.0 40.0 21.5 41.0 62-63 33.339401907446145 37.0 33.0 40.0 20.0 41.0 64-65 32.94640077782307 36.0 32.5 39.5 18.5 41.0 66-67 32.54700034977442 36.0 32.0 39.0 17.0 41.0 68-69 32.12335760654465 35.0 31.5 39.0 13.5 40.5 70-71 31.699251963818785 35.0 31.0 38.0 10.0 40.0 72-73 31.28617389105775 35.0 31.0 37.5 7.5 40.0 74-75 30.838303301081986 35.0 30.5 37.0 6.5 39.0 76-77 30.391079992722375 35.0 30.0 36.0 2.0 39.0 78-79 29.9585197199345 34.0 30.0 36.0 2.0 38.5 80-81 29.494906568271663 34.0 29.0 35.5 2.0 37.0 82-83 29.086141626447432 34.0 29.0 35.0 2.0 37.0 84-85 28.730144243063922 34.0 29.0 35.0 2.0 36.5 86-87 28.323342306174514 34.0 28.5 35.0 2.0 36.0 88-89 27.860598978745507 34.0 27.0 35.0 2.0 36.0 90-91 27.404733945372254 34.0 26.0 35.0 2.0 35.0 92-93 26.936334906655084 33.0 25.5 35.0 2.0 35.0 94-95 26.410786272638127 33.0 25.0 35.0 2.0 35.0 96-97 25.79936349736801 33.0 23.5 35.0 2.0 35.0 98-99 25.024929927560162 32.5 16.0 35.0 2.0 35.0 100-101 22.536454216988187 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16882470881710887 1101 2 0.21866925634922296 1101 3 0.29608165414166265 1101 4 0.04616683215669326 1101 5 0.07793164407463138 1101 6 0.24918323085719862 1101 7 0.27903580252745996 1101 8 0.27928668807822987 1101 9 0.26975333218097575 1101 10-11 0.21421962366516567 1101 12-13 0.2429590883183792 1101 14-15 0.27252743842311844 1101 16-17 0.38442247336535473 1101 18-19 0.4149204527891399 1101 20-21 0.523109105957964 1101 22-23 0.4830772790467037 1101 24-25 0.4218703840095017 1101 26-27 0.37142861201061805 1101 28-29 0.395332905941153 1101 30-31 0.42980288970104397 1101 32-33 0.39856827104451753 1101 34-35 0.3802917347274999 1101 36-37 0.48969147197338714 1101 38-39 0.3776822572423768 1101 40-41 0.44085147310735806 1101 42-43 0.5776525576002598 1101 44-45 0.6301922217611278 1101 46-47 0.5402132797930363 1101 48-49 0.5037058080507322 1101 50-51 0.5315419851947141 1101 52-53 0.48130363005107313 1101 54-55 0.5435911064017773 1101 56-57 0.4151459650203293 1101 58-59 0.43283987219144393 1101 60-61 0.4035831905728102 1101 62-63 0.3034769623543454 1101 64-65 0.2516782560724309 1101 66-67 0.21548461876773217 1101 68-69 0.2984712033254624 1101 70-71 0.1954449335085755 1101 72-73 0.15474104375528341 1101 74-75 0.054022064447309504 1101 76-77 0.00667963003747829 1101 78-79 -0.1858813445359253 1101 80-81 -0.23164337340206487 1101 82-83 -0.18583919263808824 1101 84-85 -0.19600389020272502 1101 86-87 -0.27544625864089767 1101 88-89 -0.32863807725719596 1101 90-91 -0.4043067006918477 1101 92-93 -0.42410486093622524 1101 94-95 -0.5641765011324082 1101 96-97 -0.6024561528453276 1101 98-99 -0.5520597851671596 1101 100-101 -0.19171670227411042 1102 1 0.09499650654446157 1102 2 0.14188182716840103 1102 3 0.1587203406302855 1102 4 0.26365161420440586 1102 5 0.2362230922459787 1102 6 0.28433113915453134 1102 7 0.2090701502996808 1102 8 0.3020396230056974 1102 9 0.40400890342659324 1102 10-11 0.36448183516065313 1102 12-13 0.3626957953266796 1102 14-15 0.5249069525575152 1102 16-17 0.5642637774855146 1102 18-19 0.600123291511359 1102 20-21 0.562582810883363 1102 22-23 0.4756232032776282 1102 24-25 0.41417339939891207 1102 26-27 0.42808937950330517 1102 28-29 0.5064826356697765 1102 30-31 0.6408977232293438 1102 32-33 0.5941289173138031 1102 34-35 0.5035086178417103 1102 36-37 0.5689829755885327 1102 38-39 0.5645628867553185 1102 40-41 0.6458303770862344 1102 42-43 0.7039820278929909 1102 44-45 0.7240375842300537 1102 46-47 0.7225417343007194 1102 48-49 0.5812320428901003 1102 50-51 0.6691490824109465 1102 52-53 0.5661004114361958 1102 54-55 0.4885019308783569 1102 56-57 0.5539417668984328 1102 58-59 0.5036655021458785 1102 60-61 0.505619723241189 1102 62-63 0.33478863929636304 1102 64-65 0.3091269243604202 1102 66-67 0.2652683537491356 1102 68-69 0.37043177115204173 1102 70-71 0.272883553464645 1102 72-73 0.2714032897491272 1102 74-75 0.19452763997099964 1102 76-77 0.23825358692410958 1102 78-79 0.25211259292484556 1102 80-81 0.21823949302836 1102 82-83 0.18939399642729882 1102 84-85 0.21237311937809977 1102 86-87 0.08174267943249092 1102 88-89 0.14069407829481406 1102 90-91 -0.009962669216200482 1102 92-93 0.016015517121996226 1102 94-95 -0.01404552372678225 1102 96-97 -0.1803818054564239 1102 98-99 -0.47378303986841885 1102 100-101 -0.19365076659849834 1103 1 0.1365018354298364 1103 2 0.26925694495626473 1103 3 0.3330930525672038 1103 4 0.30236489688444834 1103 5 0.34311997777241743 1103 6 0.29472568110867314 1103 7 0.26551213097705073 1103 8 0.32381981731505505 1103 9 0.3412265171798836 1103 10-11 0.314262675272424 1103 12-13 0.3829601816925319 1103 14-15 0.46856942399058 1103 16-17 0.4899248787884929 1103 18-19 0.5207242194630979 1103 20-21 0.5260895755621675 1103 22-23 0.522992269206334 1103 24-25 0.4680258071452954 1103 26-27 0.4914274503005842 1103 28-29 0.49973476194377753 1103 30-31 0.6214348189597416 1103 32-33 0.5404345923871787 1103 34-35 0.6233423169492269 1103 36-37 0.7246357098916008 1103 38-39 0.7897278236101855 1103 40-41 0.7733537281915446 1103 42-43 0.8201472274012644 1103 44-45 0.7734662623253072 1103 46-47 0.8405054156994396 1103 48-49 0.8402801984946393 1103 50-51 0.8047727691439732 1103 52-53 0.7056616144158241 1103 54-55 0.7444746893888734 1103 56-57 0.73463125913797 1103 58-59 0.7168643091037552 1103 60-61 0.6949943949244428 1103 62-63 0.6238010794547222 1103 64-65 0.5779766378786846 1103 66-67 0.5136248576699813 1103 68-69 0.46269975952489517 1103 70-71 0.46555071745784105 1103 72-73 0.48609218086440364 1103 74-75 0.37653737168544765 1103 76-77 0.3575075192786734 1103 78-79 0.21772918192373325 1103 80-81 0.2285239811791726 1103 82-83 0.30758997038399727 1103 84-85 0.3432468751591813 1103 86-87 0.24779511737047244 1103 88-89 0.1899427914285674 1103 90-91 0.3436944746853676 1103 92-93 0.22298505542065428 1103 94-95 0.2316034163178422 1103 96-97 0.187890036745749 1103 98-99 0.21170989556759068 1103 100-101 0.4916626438881444 1104 1 0.22827494086421396 1104 2 0.2981694029585036 1104 3 0.27672889198910156 1104 4 0.29065718796225326 1104 5 0.29790538921714216 1104 6 0.3712031023128759 1104 7 0.38587537827053353 1104 8 0.36039919974799517 1104 9 0.43549798936803086 1104 10-11 0.421817439018362 1104 12-13 0.4488953318178588 1104 14-15 0.5715172667722968 1104 16-17 0.6004196524005678 1104 18-19 0.5668765913366016 1104 20-21 0.6590077365935727 1104 22-23 0.5152276833175833 1104 24-25 0.47705032583763085 1104 26-27 0.49682041267592325 1104 28-29 0.567387070487321 1104 30-31 0.6285925205489846 1104 32-33 0.6343694991921538 1104 34-35 0.6777648310818094 1104 36-37 0.7692866618408374 1104 38-39 0.7558667961983119 1104 40-41 0.7561873937293839 1104 42-43 0.7019743632873485 1104 44-45 0.779430527564493 1104 46-47 0.8519903865010008 1104 48-49 0.8196252167603362 1104 50-51 0.8327151122190131 1104 52-53 0.840531504688343 1104 54-55 0.810126021317835 1104 56-57 0.7738948317474339 1104 58-59 0.795411786571627 1104 60-61 0.7346345307838575 1104 62-63 0.8023241415580742 1104 64-65 0.8404166410785123 1104 66-67 0.7213978597765447 1104 68-69 0.7286221603301826 1104 70-71 0.8242019227268891 1104 72-73 0.6965112918451482 1104 74-75 0.5691557653711179 1104 76-77 0.5777373570206628 1104 78-79 0.43777102969184867 1104 80-81 0.46880058309373496 1104 82-83 0.4162365925641467 1104 84-85 0.5169479444354863 1104 86-87 0.48458990498179944 1104 88-89 0.646530523416196 1104 90-91 0.5833921908071744 1104 92-93 0.5870702208784913 1104 94-95 0.6100249838342435 1104 96-97 0.5551979469905319 1104 98-99 0.807739107342961 1104 100-101 1.1488659263797452 1105 1 0.1476500325038046 1105 2 0.18450702066558122 1105 3 0.16380319944328292 1105 4 0.25837027746735686 1105 5 0.2623952437992969 1105 6 0.2897625110812072 1105 7 0.2735505678145955 1105 8 0.37476226779072164 1105 9 0.3377069802598385 1105 10-11 0.3898121225944067 1105 12-13 0.3782416637154853 1105 14-15 0.4375017137536332 1105 16-17 0.511094378898143 1105 18-19 0.5047714503295282 1105 20-21 0.5198842995364359 1105 22-23 0.5172414285718432 1105 24-25 0.4764716384336012 1105 26-27 0.5058738487561101 1105 28-29 0.6044942836958498 1105 30-31 0.5080615348200652 1105 32-33 0.5077506350611216 1105 34-35 0.5056952418090432 1105 36-37 0.6232598888371044 1105 38-39 0.6523835824786204 1105 40-41 0.744107593935432 1105 42-43 0.55248616888057 1105 44-45 0.6579525256527532 1105 46-47 0.7264064642734951 1105 48-49 0.7900972071150605 1105 50-51 0.8392805867837225 1105 52-53 0.7856600447528095 1105 54-55 0.731669078284142 1105 56-57 0.5385970568009029 1105 58-59 0.5917753506551193 1105 60-61 0.6984721448696192 1105 62-63 0.6097258198239714 1105 64-65 0.590387614163383 1105 66-67 0.6330119091575455 1105 68-69 0.7060620266534698 1105 70-71 0.5395185253337011 1105 72-73 0.523969095788015 1105 74-75 0.5579683561154347 1105 76-77 0.46659572128679727 1105 78-79 0.5141548699647949 1105 80-81 0.5031561220037268 1105 82-83 0.5320314673138533 1105 84-85 0.6544417378903589 1105 86-87 0.6325189186831146 1105 88-89 0.6146062602467737 1105 90-91 0.7804837534627396 1105 92-93 0.751200759752269 1105 94-95 0.7086837745795549 1105 96-97 0.7313498938378835 1105 98-99 0.8402784803776875 1105 100-101 1.2376508985011903 1106 1 0.14645722207887957 1106 2 0.16901528930523924 1106 3 0.19097887543567893 1106 4 0.2407323787086355 1106 5 0.2558967457829979 1106 6 0.2348962858674284 1106 7 0.2904481025566952 1106 8 0.25850669768517776 1106 9 0.25842744249382577 1106 10-11 0.31959768849119286 1106 12-13 0.26398401678836336 1106 14-15 0.34588890515382786 1106 16-17 0.4476330744262995 1106 18-19 0.3464130413401634 1106 20-21 0.4095485560644718 1106 22-23 0.3691887744938427 1106 24-25 0.3008703213554753 1106 26-27 0.16001944468260376 1106 28-29 0.19585071534761056 1106 30-31 0.08631316496106223 1106 32-33 0.24603398101903196 1106 34-35 0.249309972269792 1106 36-37 0.4295865525882334 1106 38-39 0.4617670255269104 1106 40-41 0.5915264396918758 1106 42-43 0.5903399322922098 1106 44-45 0.5665563565090466 1106 46-47 0.6638737236967955 1106 48-49 0.6799137906955721 1106 50-51 0.6896723891043237 1106 52-53 0.44281565440260096 1106 54-55 0.4417503833743268 1106 56-57 0.6179725099641473 1106 58-59 0.7389943900687328 1106 60-61 0.7944584150830636 1106 62-63 0.7313502367580895 1106 64-65 0.7820269518244842 1106 66-67 0.7600743784895485 1106 68-69 0.8396505609128653 1106 70-71 0.7445690171934665 1106 72-73 0.7708864637460948 1106 74-75 0.6488275237013106 1106 76-77 0.6747776365952625 1106 78-79 0.6712249271319273 1106 80-81 0.6879671404653571 1106 82-83 0.6648995374692568 1106 84-85 0.8563066074790768 1106 86-87 0.8540915896110555 1106 88-89 1.0467576873307465 1106 90-91 1.0533885840706958 1106 92-93 1.0878551396903156 1106 94-95 1.1135783832709159 1106 96-97 1.0673493064086692 1106 98-99 1.0898018486409526 1106 100-101 1.41629602954578 1107 1 0.03153675315155269 1107 2 0.10956099095329819 1107 3 0.15425842180850324 1107 4 0.14091158605191367 1107 5 0.14235960355398447 1107 6 0.014342823973223062 1107 7 0.05459062197154907 1107 8 0.09360963699755587 1107 9 0.1016457437017948 1107 10-11 0.15971031294727567 1107 12-13 0.11421193146700404 1107 14-15 0.12950123722353624 1107 16-17 0.23141669762073747 1107 18-19 0.29984535959022907 1107 20-21 0.2008476044347418 1107 22-23 0.2232861665114214 1107 24-25 0.08156705256995878 1107 26-27 0.060251540288241756 1107 28-29 0.14035378354765982 1107 30-31 0.06285701945019895 1107 32-33 0.17285455964695728 1107 34-35 0.2571695889341754 1107 36-37 0.18628739330426924 1107 38-39 0.07535741190769585 1107 40-41 0.030037072801199827 1107 42-43 0.1055070263975395 1107 44-45 0.23389648974414 1107 46-47 0.38718766507989244 1107 48-49 0.3060830112247075 1107 50-51 0.296141876940446 1107 52-53 0.2983806570169989 1107 54-55 0.28326106525598505 1107 56-57 0.44350838349379273 1107 58-59 0.4324013343316224 1107 60-61 0.4185595312513115 1107 62-63 0.2853164262729777 1107 64-65 0.2795868192330815 1107 66-67 0.26948658595463826 1107 68-69 0.3598252202988377 1107 70-71 0.4249090946003413 1107 72-73 0.4067056258722346 1107 74-75 0.447855716580257 1107 76-77 0.23202362562867407 1107 78-79 0.26594864058239054 1107 80-81 0.23539332033471894 1107 82-83 0.15879360460077052 1107 84-85 0.3959949931795279 1107 86-87 0.38100063615916113 1107 88-89 0.43606877454259774 1107 90-91 0.685623769539216 1107 92-93 0.7874133230920783 1107 94-95 0.7263606221483521 1107 96-97 0.8406635913024196 1107 98-99 1.1168654440376393 1107 100-101 1.5102370183761735 1108 1 0.18200185797622837 1108 2 0.20158762157340604 1108 3 0.07831173845091044 1108 4 0.2067576380744427 1108 5 0.281652179652788 1108 6 0.1678249500420037 1108 7 0.10589200945201327 1108 8 0.1488914021007517 1108 9 0.21837384937838067 1108 10-11 0.39544186551885474 1108 12-13 0.39949683084817167 1108 14-15 0.4758017419848528 1108 16-17 0.5169695544586901 1108 18-19 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Count 2 24945.0 3 5640.0 4 5256.0 5 6244.0 6 7674.0 7 9226.0 8 11544.0 9 13720.0 10 17185.0 11 21269.0 12 26756.0 13 32372.0 14 34710.0 15 38500.0 16 45167.0 17 54519.0 18 48925.0 19 38015.0 20 38756.0 21 41784.0 22 45505.0 23 51100.0 24 58423.0 25 66842.0 26 76261.0 27 89271.0 28 103887.0 29 123132.0 30 145746.0 31 176087.0 32 215439.0 33 270768.0 34 355735.0 35 493072.0 36 719248.0 37 1014155.0 38 863140.0 39 139229.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.812441672744974 19.080561160331992 14.28797859178919 22.819018575133846 2 15.140208925292859 20.966132291575146 40.082262182855374 23.81139660027662 3 19.048051363707447 25.709307089727567 30.219161217218826 25.023480329346164 4 12.539848796483922 16.635248961911454 38.17406918030043 32.6508330613042 5 13.109424037451923 38.022878826552805 34.44367056114073 14.424026574854537 6 30.64549125929153 36.84057360168869 17.861404417023657 14.65253072199613 7 27.28851433568817 33.534643794153176 21.71125433826974 17.46558753188891 8 25.94810382860314 34.01372853002185 20.598632948653353 19.43953469272166 9 26.556361033449228 16.82827140467343 21.01790155701308 35.597466004864266 10-11 24.682293897547343 26.418000804102466 27.79995337039357 21.09975192795662 12-13 26.086438418331397 24.127766412160614 27.985519470727937 21.80027569878005 14-15 22.800936703659673 25.425949792944717 26.81653007310796 24.956583430287647 16-17 21.6277395642815 28.583742573639675 27.43347744105888 22.355040421019943 18-19 21.649837832664193 27.260508610132035 29.674664698277393 21.41498885892638 20-21 22.773082099840124 26.18286460364899 29.436523177802876 21.607530118708006 22-23 22.236511394519574 26.18319649539693 29.530524998814776 22.049767111268718 24-25 22.304969276587215 26.39815229901258 29.49583583870648 21.80104258569373 26-27 21.813339159987294 26.31437490035723 29.83452199917264 22.037763940482836 28-29 21.67438673300635 27.145152033283416 29.423499136283276 21.756962097426957 30-31 21.921577191421317 26.505669750243683 29.199422341707155 22.373330716627844 32-33 21.61120253232794 26.692637681243458 29.307575411782793 22.388584374645806 34-35 22.439681456960134 26.6310047639152 29.106285805144505 21.823027973980157 36-37 22.41740334734651 26.11933895621789 29.36580620741106 22.097451489024532 38-39 21.917274143186237 26.65636257711047 29.256455275791282 22.16990800391201 40-41 22.11766819577762 26.731397165208865 29.148859427806105 22.002075211207412 42-43 21.94305730350694 26.67457575272094 28.669680530713592 22.712686413058524 44-45 22.01322868567011 26.575511190423757 28.911283272562816 22.49997685134332 46-47 22.586954642906946 26.994113934531004 28.324415932648506 22.094515489913544 48-49 22.269742902536034 27.050445823806317 28.149884549898967 22.529926723758674 50-51 22.00317963580368 27.260455935441225 28.39083371467172 22.345530714083374 52-53 22.478945455526034 27.234530061259903 27.849907385154367 22.436617098059696 54-55 22.06003676409506 27.005558374553807 28.1549470332472 22.779457828103933 56-57 22.15985150357172 27.251318405024914 27.8071543328099 22.781675758593458 58-59 22.403110468793585 26.74628564645986 28.058476101873914 22.79212778287264 60-61 21.920648362379705 27.09245232782977 28.567115693658934 22.41978361613159 62-63 21.8546071509948 27.00468690001008 28.58541496267637 22.555290986318756 64-65 21.705405167829504 27.287414385303187 28.22387280641852 22.783307640448786 66-67 21.837388075777 27.104151780670843 27.923706448586145 23.13475369496601 68-69 22.009205929335597 26.861803984707215 28.239841677559614 22.88914840839757 70-71 22.22543517701741 26.694865188644894 27.98081805441641 23.098881579921276 72-73 22.04142019894438 26.687248602015163 28.181329290200203 23.090001908840254 74-75 21.738050632886104 27.517962109739607 27.820400605240213 22.923586652134073 76-77 21.939349116052313 26.89968107774242 27.91832205509882 23.24264775110645 78-79 22.031782073046106 27.044415336801258 27.93018244053943 22.993620149613207 80-81 22.331939762519557 26.883565047954967 27.73877060456706 23.04572458495842 82-83 22.237819287032565 26.658134581970067 28.102052375815177 23.001993755182188 84-85 21.981052679361763 26.930588529017907 27.952872284890717 23.135486506729613 86-87 22.102593394973276 26.848417592585193 27.83060901823707 23.21837999420446 88-89 22.031611304197586 26.802305464933884 27.56896888432192 23.59711434654661 90-91 21.975769486749115 26.578900457801286 27.83773821663183 23.60759183881777 92-93 22.379399707846996 26.533871386931285 27.86299865656112 23.2237302486606 94-95 22.10797573536123 26.315190555699097 28.03720069366461 23.53963301527506 96-97 21.977404813978143 26.0035266493979 28.373843363075057 23.645225173548894 98-99 22.065547901402432 26.319893389576134 27.77141058472083 23.843148124300605 100-101 22.34871643275129 25.520097269188597 28.312691533669344 23.818494764390778 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4999.0 1 3413.5 2 1826.0 3 1886.5 4 1799.0 5 1849.5 6 2525.0 7 4107.0 8 6226.5 9 7105.0 10 6441.5 11 5965.0 12 6151.5 13 6473.0 14 6925.0 15 7411.0 16 7924.5 17 8567.0 18 9272.0 19 9971.0 20 10933.0 21 12693.5 22 14812.0 23 17637.5 24 21291.0 25 26212.0 26 33210.5 27 41946.0 28 50506.5 29 59265.0 30 68768.5 31 81327.0 32 98195.5 33 107488.5 34 110704.0 35 118407.0 36 131651.0 37 143650.0 38 150382.5 39 154987.5 40 158441.0 41 162324.0 42 163747.0 43 168509.5 44 176179.5 45 184154.0 46 191869.0 47 204921.0 48 229945.5 49 227889.0 50 200270.5 51 187373.0 52 185427.5 53 186047.0 54 182619.0 55 170281.0 56 156645.5 57 141567.5 58 129583.0 59 117565.0 60 98794.0 61 81839.0 62 68025.5 63 51117.0 64 35603.0 65 26371.5 66 19490.5 67 14986.5 68 11506.0 69 8693.5 70 6452.0 71 4479.5 72 3351.0 73 2757.5 74 2134.0 75 1598.5 76 1171.5 77 774.5 78 563.5 79 421.5 80 285.5 81 232.0 82 203.0 83 154.0 84 124.0 85 98.5 86 69.5 87 46.0 88 35.5 89 32.5 90 23.5 91 15.5 92 14.0 93 11.5 94 7.5 95 8.0 96 7.0 97 3.5 98 2.5 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27367045028302067 2 0.16499441699259831 3 0.19584835488467028 4 0.16342097467336603 5 0.23666742746521335 6 0.19306318112419016 7 0.2397057988402826 8 0.24616233801230467 9 0.14819294671022148 10-11 0.20480974188673456 12-13 0.21091361295272185 14-15 0.17174032486700794 16-17 0.2859324490466929 18-19 0.2306630268906718 20-21 0.19723189899296073 22-23 0.24318726604088273 24-25 0.23151304745393522 26-27 0.3430556394523842 28-29 0.3136666306161492 30-31 0.2890793336851574 32-33 0.19101047189162854 34-35 0.36069808752607485 36-37 0.4014900317907691 38-39 0.38176774616866793 40-41 0.3435439491376632 42-43 0.3770022053512231 44-45 0.38701255389944217 46-47 0.3923477893497126 48-49 0.4121062460596121 50-51 0.4043565904987957 52-53 0.4038773235854663 54-55 0.3979271796426224 56-57 0.37487715394306453 58-59 0.35029889978402245 60-61 0.3173199104837919 62-63 0.38654232975806246 64-65 0.41983781607652937 66-67 0.42580604556327245 68-69 0.43051732974901963 70-71 0.43022796104663213 72-73 0.42170062709814915 74-75 0.4165914609466191 76-77 0.4003506425251181 78-79 0.3726797603593091 80-81 0.3842364229109117 82-83 0.39483455163585557 84-85 0.3961276680246499 86-87 0.3905392349597904 88-89 0.3997357340325446 90-91 0.42114901800922294 92-93 0.42065166555199435 94-95 0.42926942721997335 96-97 0.42987529294059734 98-99 0.42727097461910934 100-101 0.39945540810210667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5529278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.46882338422909 #Duplication Level Percentage of deduplicated Percentage of total 1 64.9249188025825 23.02810478244213 2 14.337033818017323 10.170354406899522 3 6.648073475402036 7.073980318332388 4 3.7544978792767263 5.326704887061155 5 2.397276925953433 4.251429594486498 6 1.6392089543775512 3.4884487735598526 7 1.1511228970212526 2.858028230959226 8 0.8521697826426761 2.4180367611143976 9 0.6423077750060266 2.0503710927005323 >10 3.3275318502884255 20.815650170496934 >50 0.20165481052940382 4.934985438650117 >100 0.11286965956752368 7.368314937080475 >500 0.006666692379138713 1.6160562050734462 >1k 0.0042564196411418636 2.907103930832806 >5k 2.0512865740388123E-4 0.5375448454430112 >10k+ 2.0512865740388123E-4 1.1548856248676314 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21110 0.38178583171256714 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17739 0.3208194632282913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14512 0.2624574130655033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10132 0.18324273078691286 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9719 0.17577340115653436 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7660 0.13853526626803717 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6986 0.12634560967996183 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8085543899221563E-5 2 0.0 0.0 0.0 0.0 1.8085543899221563E-5 3 0.0 0.0 0.0 0.0 1.8085543899221563E-5 4 0.0 0.0 0.0 0.0 1.8085543899221563E-5 5 0.0 0.0 0.0 0.0 1.8085543899221563E-5 6 0.0 0.0 0.0 0.0 1.8085543899221563E-5 7 0.0 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 8 1.8085543899221563E-5 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 9 1.8085543899221563E-5 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 10-11 1.8085543899221563E-5 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 12-13 1.8085543899221563E-5 0.0 0.0 2.7128315848832347E-5 9.947049144571859E-5 14-15 1.8085543899221563E-5 0.0 0.0 4.521385974805391E-5 1.0851326339532937E-4 16-17 1.8085543899221563E-5 0.0 0.0 1.6276989509299405E-4 1.0851326339532937E-4 18-19 1.8085543899221563E-5 0.0 0.0 3.0745424628676656E-4 1.3564157924416173E-4 20-21 1.8085543899221563E-5 0.0 0.0 4.792669133293714E-4 1.8085543899221563E-4 22-23 1.8085543899221563E-5 0.0 0.0 8.68106107162635E-4 1.9894098289143717E-4 24-25 1.8085543899221563E-5 0.0 0.0 0.0014106724241392818 1.9894098289143717E-4 26-27 1.8085543899221563E-5 0.0 0.0 0.0024325056544452998 2.0798375484104798E-4 28-29 1.8085543899221563E-5 0.0 0.0 0.007351773595033565 2.1702652679065875E-4 30-31 1.8085543899221563E-5 0.0 0.0 0.021639353275418598 2.1702652679065875E-4 32-33 1.8085543899221563E-5 0.0 0.0 0.05159805674447912 2.351120706898803E-4 34-35 1.8085543899221563E-5 0.0 0.0 0.0947772928038706 2.4415484263949107E-4 36-37 1.8085543899221563E-5 0.0 0.0 0.16230871372356392 2.5319761458910187E-4 38-39 1.8085543899221563E-5 0.0 0.0 0.2683985142363976 2.6224038653871267E-4 40-41 1.8085543899221563E-5 0.0 0.0 0.39126265671575927 2.712831584883234E-4 42-43 1.8085543899221563E-5 0.0 0.0 0.5150401191620316 2.712831584883234E-4 44-45 1.8085543899221563E-5 0.0 0.0 0.6683331892518336 2.712831584883234E-4 46-47 1.8085543899221563E-5 0.0 0.0 0.8510514392656691 2.712831584883234E-4 48-49 1.8085543899221563E-5 0.0 0.0 1.0412661472257319 2.712831584883234E-4 50-51 1.8085543899221563E-5 0.0 0.0 1.259305464474747 2.712831584883234E-4 52-53 1.8085543899221563E-5 0.0 0.0 1.4760968791947158 3.255397901859881E-4 54-55 1.8085543899221563E-5 0.0 0.0 1.7089753852130423 3.4362533408520966E-4 56-57 1.8085543899221563E-5 0.0 0.0 1.9879810709463333 3.4362533408520966E-4 58-59 1.8085543899221563E-5 0.0 0.0 2.2887346232184385 3.5266810603482046E-4 60-61 1.8085543899221563E-5 0.0 0.0 2.622440036474925 3.6171087798443126E-4 62-63 1.8085543899221563E-5 0.0 0.0 2.957465694436055 3.797964218836528E-4 64-65 1.8085543899221563E-5 0.0 0.0 3.302049562347923 3.797964218836528E-4 66-67 1.8085543899221563E-5 0.0 0.0 3.6985570267944565 3.797964218836528E-4 68-69 1.8085543899221563E-5 0.0 0.0 4.140215051585397 3.797964218836528E-4 70-71 1.8085543899221563E-5 0.0 0.0 4.614255242727893 3.797964218836528E-4 72-73 1.8085543899221563E-5 0.0 0.0 5.10222130267279 4.0692473773248515E-4 74-75 1.8085543899221563E-5 0.0 0.0 5.604691968824863 4.340530535813175E-4 76-77 1.8085543899221563E-5 0.0 0.0 6.1316595041884305 4.5213859748053904E-4 78-79 1.8085543899221563E-5 0.0 0.0 6.735472515579792 4.6118136943014984E-4 80-81 1.8085543899221563E-5 0.0 0.0 7.377572623405804 4.702241413797606E-4 82-83 1.8085543899221563E-5 0.0 0.0 8.042668500299678 4.883096852789821E-4 84-85 1.8085543899221563E-5 0.0 0.0 8.710006984637054 4.883096852789821E-4 86-87 1.8085543899221563E-5 0.0 0.0 9.39453577845064 5.154380011278144E-4 88-89 1.8085543899221563E-5 0.0 0.0 10.121637942602995 5.425663169766468E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 25625 0.0 26.22099 1 TACATGG 25320 0.0 25.854374 2 ACATGGG 25230 0.0 24.856316 3 GTATCAA 43650 0.0 23.285742 1 CATGGGG 12270 0.0 21.534422 4 AGAGTAC 33080 0.0 21.312506 10-11 GAGTACT 20345 0.0 21.311872 12-13 AGTACTT 21160 0.0 21.051804 12-13 CAACGCA 50490 0.0 20.049873 5 ATCAACG 50545 0.0 20.046827 3 TATCAAC 50640 0.0 20.027773 2 TCAACGC 50680 0.0 20.012146 4 GTACTTT 21725 0.0 19.849323 14-15 AACGCAG 51465 0.0 19.66914 6 CATGGGA 12420 0.0 19.21184 4 GGTATCA 33195 0.0 18.55222 1 ACGCAGA 55340 0.0 18.034887 7 CGCAGAG 55425 0.0 17.87885 8 TACTTTT 25190 0.0 17.495821 14-15 TAAGGTG 2500 0.0 17.457031 5 >>END_MODULE