FastQCFastQC Report
Thu 26 May 2016
SRR938816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5529278
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201490.36440562402541526No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT187140.3384528685300323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155800.28177277394987194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105350.19053120497829915No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT91250.16503058808039675No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71690.1296552642135194No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC66250.11981672833234285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG258500.025.4554441
TACATGG256950.025.2488462
ACATGGG251600.024.371013
GTATCAA449800.021.7799031
CATGGGG116750.021.4227584
GAGTACT218450.020.77554712-13
AGTACTT224700.020.4616312-13
AGAGTAC354200.019.93975410-11
GTACTTT225350.019.79200714-15
TAAGGTG28400.019.7190195
GGTATCA347800.019.3847121
CAACGCA518150.018.7492545
TCAACGC518450.018.7384054
TATCAAC522800.018.7368162
ATCAACG518600.018.7329853
CATGGGA123000.018.636474
AACGCAG529000.018.3960936
TACTTTT256950.017.5703214-15
GGTAAGG26300.017.5040153
AGGTAAG23900.017.4745672
Produced by FastQC (version 0.11.5)