##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938816_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5529278 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08268005334512 33.0 31.0 34.0 30.0 34.0 2 32.27612086062592 34.0 31.0 34.0 30.0 34.0 3 32.359505888472235 34.0 31.0 34.0 30.0 34.0 4 35.97948430156704 37.0 35.0 37.0 35.0 37.0 5 35.88502820802282 37.0 35.0 37.0 35.0 37.0 6 35.914642924446916 37.0 35.0 37.0 35.0 37.0 7 35.832086214511186 37.0 35.0 37.0 35.0 37.0 8 35.819930739601084 37.0 35.0 37.0 33.0 37.0 9 37.463664514607515 39.0 37.0 39.0 34.0 39.0 10-11 37.39462457847118 39.0 37.0 39.0 34.0 39.0 12-13 37.320834655085164 39.0 37.0 39.0 34.0 39.0 14-15 38.54159729353452 40.0 38.0 41.0 34.0 41.0 16-17 38.48568836654623 40.0 38.0 41.0 34.0 41.0 18-19 38.627635380243134 40.0 38.0 41.0 34.0 41.0 20-21 38.62246327639883 40.0 38.0 41.0 34.0 41.0 22-23 38.545587687940454 40.0 38.0 41.0 34.0 41.0 24-25 38.463114623645254 40.0 38.0 41.0 34.0 41.0 26-27 38.285636207837626 40.0 38.0 41.0 33.5 41.0 28-29 38.122500803902426 40.0 38.0 41.0 33.0 41.0 30-31 37.89821727176677 40.0 37.0 41.0 33.0 41.0 32-33 37.7305462847048 40.0 37.0 41.0 33.0 41.0 34-35 37.68096214370122 40.0 37.0 41.0 33.0 41.0 36-37 37.56129633923272 40.0 37.0 41.0 32.0 41.0 38-39 37.42910385406557 40.0 37.0 41.0 32.0 41.0 40-41 37.255165231337614 40.0 36.0 41.0 31.0 41.0 42-43 37.045447977113824 40.0 36.0 41.0 31.0 41.0 44-45 36.787420256315556 40.0 36.0 41.0 30.5 41.0 46-47 36.46862357074468 39.0 35.5 41.0 30.0 41.0 48-49 36.151693584587356 39.0 35.0 41.0 29.5 41.0 50-51 35.908813139798724 39.0 35.0 41.0 28.5 41.0 52-53 35.68377679689826 39.0 35.0 40.5 28.0 41.0 54-55 35.40754877942473 38.5 35.0 40.0 27.5 41.0 56-57 35.12412660387125 38.0 34.0 40.0 27.0 41.0 58-59 34.812849073604184 38.0 34.0 40.0 26.0 41.0 60-61 34.44477163202863 37.5 34.0 40.0 26.0 41.0 62-63 34.088748657600505 37.0 33.5 40.0 25.5 41.0 64-65 33.69140500803179 36.0 33.0 39.0 25.0 41.0 66-67 33.283316194266234 36.0 33.0 39.0 23.5 40.0 68-69 32.950193949372775 35.5 32.0 39.0 23.0 40.0 70-71 32.52415288216653 35.0 32.0 38.0 22.0 40.0 72-73 32.0591932617604 35.0 31.5 37.0 20.5 39.5 74-75 31.588350142640685 35.0 31.0 37.0 19.5 39.0 76-77 29.647940834228265 33.0 28.5 35.0 17.5 37.5 78-79 30.604872643408417 34.0 30.5 36.0 17.5 38.0 80-81 30.668790753512482 34.0 31.0 35.5 18.0 37.0 82-83 30.37212136919142 34.0 30.5 35.0 14.5 37.0 84-85 29.984872256377777 34.0 30.0 35.0 8.5 36.5 86-87 29.43898208771561 34.0 29.5 35.0 4.5 36.0 88-89 28.849344163921582 34.0 29.0 35.0 2.0 35.5 90-91 28.346615688341224 34.0 28.5 35.0 2.0 35.0 92-93 28.07455901114033 33.0 28.0 35.0 2.0 35.0 94-95 27.75183785297104 33.0 27.0 35.0 2.0 35.0 96-97 27.172232161233346 33.0 26.5 35.0 2.0 35.0 98-99 26.37399819650956 33.0 25.0 35.0 2.0 35.0 100-101 22.76502212404585 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05153336319041557 1101 2 0.1546617659776217 1101 3 0.02962354071411255 1101 4 0.07801857790471445 1101 5 -0.03735926202968898 1101 6 -0.007518360359000553 1101 7 0.15013482496917874 1101 8 0.17599250020018786 1101 9 0.1937895919815773 1101 10-11 0.18412172605586363 1101 12-13 0.14242267004657805 1101 14-15 0.26533976616182287 1101 16-17 0.254489769515466 1101 18-19 0.22017225554144204 1101 20-21 0.18285091259711095 1101 22-23 0.11462484513153015 1101 24-25 0.14892881207527608 1101 26-27 0.062454517670879284 1101 28-29 0.13687454225034656 1101 30-31 0.13144928009202772 1101 32-33 0.1382749485584398 1101 34-35 0.18975594737126045 1101 36-37 0.2196444299201019 1101 38-39 0.22154711598977173 1101 40-41 0.27752335963801755 1101 42-43 0.3531284516559339 1101 44-45 0.29133147562375683 1101 46-47 0.26778570054539585 1101 48-49 0.2128086266277549 1101 50-51 0.1858863431440554 1101 52-53 0.05259267495105746 1101 54-55 0.1641483214752455 1101 56-57 0.10792716734235341 1101 58-59 0.07554834796035692 1101 60-61 0.18043173326116602 1101 62-63 0.10596559812136519 1101 64-65 0.09566472171236029 1101 66-67 0.09681849794226594 1101 68-69 0.034860579887570964 1101 70-71 0.10732160868386131 1101 72-73 0.0690392959175199 1101 74-75 -0.025192382952567982 1101 76-77 -0.0563243666881732 1101 78-79 -0.1980487306196963 1101 80-81 0.0035274050463520723 1101 82-83 0.06569664206032044 1101 84-85 0.11812066551297207 1101 86-87 0.1932585081210334 1101 88-89 0.1793178887712621 1101 90-91 0.10803008640236555 1101 92-93 0.1010807062684087 1101 94-95 0.23154461382851466 1101 96-97 0.0866754165421284 1101 98-99 -0.11370905159815337 1101 100-101 0.432142401977206 1102 1 0.24217742841148038 1102 2 0.14974140311510098 1102 3 0.12633846173408614 1102 4 0.09835289713365114 1102 5 -0.0914814738400409 1102 6 -0.03992135596661939 1102 7 0.08513060508045811 1102 8 0.06003451016774619 1102 9 0.14578478137688933 1102 10-11 0.1747537955673124 1102 12-13 0.17746768219287645 1102 14-15 0.2477875274988719 1102 16-17 0.27415024130791465 1102 18-19 0.28175668856704306 1102 20-21 0.3007546033056201 1102 22-23 0.23630677367200548 1102 24-25 0.2583069243715457 1102 26-27 0.1825768670639647 1102 28-29 0.19179683880071252 1102 30-31 0.2002725848296052 1102 32-33 0.23394663642494606 1102 34-35 0.2475159907406308 1102 36-37 0.2089874141240955 1102 38-39 0.32414734433606185 1102 40-41 0.33240482628378487 1102 42-43 0.30319050079008747 1102 44-45 0.2595622847129846 1102 46-47 0.2813912994102381 1102 48-49 0.3407502792224193 1102 50-51 0.30105227409668345 1102 52-53 0.16904432009161496 1102 54-55 0.19964917058666742 1102 56-57 0.15226952181437525 1102 58-59 0.16195992982654417 1102 60-61 0.1266997670394403 1102 62-63 0.03994280387738769 1102 64-65 0.17565372193755735 1102 66-67 0.2311854359184622 1102 68-69 0.09797594610066795 1102 70-71 0.09554206142008326 1102 72-73 0.13889746590271557 1102 74-75 0.07792802453877812 1102 76-77 6.898036502214211E-4 1102 78-79 0.01805755444764756 1102 80-81 0.11113747637718774 1102 82-83 0.12912663468835106 1102 84-85 0.2562589792960921 1102 86-87 0.27782073145821684 1102 88-89 0.2958155317255553 1102 90-91 0.2864986946057222 1102 92-93 0.4078196940016383 1102 94-95 0.4602566271295494 1102 96-97 0.49098086666701235 1102 98-99 0.6550091323917293 1102 100-101 1.234053005363112 1103 1 0.14657832820243755 1103 2 0.14938008546154435 1103 3 0.12587326737057225 1103 4 -0.0027154861670055652 1103 5 -0.010831955010225158 1103 6 0.01044646032763552 1103 7 0.05049427172185261 1103 8 -2.7649717571875954E-4 1103 9 0.06073074111780841 1103 10-11 0.09069563814857418 1103 12-13 0.10078219880232098 1103 14-15 0.20609426266302222 1103 16-17 0.10300858921806366 1103 18-19 0.037379231268531043 1103 20-21 0.07511084863472206 1103 22-23 0.15775948049125077 1103 24-25 0.12324921170352354 1103 26-27 0.15556318539446323 1103 28-29 0.03984988666319822 1103 30-31 0.11394914342026397 1103 32-33 0.15437419959715015 1103 34-35 0.19859997754839043 1103 36-37 0.21032819878080744 1103 38-39 0.25693246204269116 1103 40-41 0.2499252293078129 1103 42-43 0.2131061741575735 1103 44-45 0.2736652827525603 1103 46-47 0.29109712083842254 1103 48-49 0.2793746117578664 1103 50-51 0.35348283341303244 1103 52-53 0.2532141898209517 1103 54-55 0.26492256704707273 1103 56-57 0.23942010141189485 1103 58-59 0.24484469335725123 1103 60-61 0.35942323227713047 1103 62-63 0.18866568559129604 1103 64-65 0.2175386966740902 1103 66-67 0.21400531458342442 1103 68-69 0.18737021524729158 1103 70-71 0.2569594284957972 1103 72-73 0.2220669172820564 1103 74-75 0.2264938049984515 1103 76-77 0.30167605699431377 1103 78-79 0.12173894978703714 1103 80-81 0.29012024617908594 1103 82-83 0.2342170334882674 1103 84-85 0.2825409038685329 1103 86-87 0.306241301145306 1103 88-89 0.14301786690377938 1103 90-91 0.19051559919484262 1103 92-93 0.19693374311511747 1103 94-95 0.19080822633671346 1103 96-97 0.28410359552747977 1103 98-99 0.498987414069898 1103 100-101 1.242404401758531 1104 1 0.18133397456796985 1104 2 0.18509734720758786 1104 3 0.12755584429980615 1104 4 0.08537398239467109 1104 5 -0.05899221979610303 1104 6 0.0781823477469743 1104 7 0.11965424985635309 1104 8 0.00916883240670785 1104 9 0.13397266821502996 1104 10-11 0.1705387131845697 1104 12-13 0.09694590340473752 1104 14-15 0.16197803219531437 1104 16-17 0.2730746023640265 1104 18-19 0.2269017900653978 1104 20-21 0.2205456754439581 1104 22-23 0.20872601227622312 1104 24-25 0.13388922756934818 1104 26-27 0.1632523586385659 1104 28-29 0.13633840727543856 1104 30-31 0.03975360051587984 1104 32-33 0.13044264261352367 1104 34-35 0.11582139240626788 1104 36-37 0.17040949444503894 1104 38-39 0.2372527616267135 1104 40-41 0.24054672949447564 1104 42-43 0.21004183758885375 1104 44-45 0.23811584538922403 1104 46-47 0.1518893785846771 1104 48-49 0.2731019463000237 1104 50-51 0.1723561601689383 1104 52-53 0.22910805881460305 1104 54-55 0.2021540813663094 1104 56-57 0.14246452237610185 1104 58-59 0.3107364291313459 1104 60-61 0.34198766217419774 1104 62-63 0.402588276386453 1104 64-65 0.188250842026342 1104 66-67 0.195049075582844 1104 68-69 0.15201251765783041 1104 70-71 0.07786864667082938 1104 72-73 0.1833743975497697 1104 74-75 0.18820543037842796 1104 76-77 0.12983613845663555 1104 78-79 0.07032410857856775 1104 80-81 0.1721622872702291 1104 82-83 0.2977799767979583 1104 84-85 0.20538335227128712 1104 86-87 0.34004624821479723 1104 88-89 0.30686779490762106 1104 90-91 0.31018288360634116 1104 92-93 0.41306913256101296 1104 94-95 0.4326119968022901 1104 96-97 0.42382789356491557 1104 98-99 0.7678693237013832 1104 100-101 1.508340385172751 1105 1 0.15081227391102914 1105 2 0.14619001460806658 1105 3 0.09088877472743206 1105 4 0.10647911680288757 1105 5 -0.010901888229390977 1105 6 0.010578396867565232 1105 7 0.1170817264267967 1105 8 0.13661577238078593 1105 9 0.1928648105719688 1105 10-11 0.11005907942425353 1105 12-13 0.1667640115966762 1105 14-15 0.15536795902688283 1105 16-17 0.1314467549379259 1105 18-19 0.2005684471288518 1105 20-21 0.07541761822255211 1105 22-23 0.09840809070313128 1105 24-25 0.09716880383915338 1105 26-27 0.14849606321007514 1105 28-29 0.21791343810898667 1105 30-31 0.1643808496994339 1105 32-33 0.2610772480719987 1105 34-35 0.20817779367395417 1105 36-37 0.18098499669436308 1105 38-39 0.19855628659026792 1105 40-41 0.16869337343747048 1105 42-43 0.1829395027046843 1105 44-45 0.26249150115044273 1105 46-47 0.19722523536312764 1105 48-49 0.2154865858391659 1105 50-51 0.09092924949055003 1105 52-53 0.20984725612665045 1105 54-55 0.22189170766800004 1105 56-57 0.2332019025887746 1105 58-59 0.2027199146112082 1105 60-61 0.1778105128506411 1105 62-63 0.17859203869955564 1105 64-65 0.07999837819892974 1105 66-67 0.1547925185338883 1105 68-69 0.19905834702850456 1105 70-71 0.09249092096141709 1105 72-73 0.14552008127888172 1105 74-75 0.09444704600992537 1105 76-77 0.2043660384324788 1105 78-79 -0.06778753085226441 1105 80-81 0.027061220975717504 1105 82-83 0.17201353989484147 1105 84-85 0.05180272496480498 1105 86-87 0.1119784847325711 1105 88-89 0.02436361308437185 1105 90-91 -0.06047260320488235 1105 92-93 0.10186759209852525 1105 94-95 0.2713508010800858 1105 96-97 0.4100432232203808 1105 98-99 0.5635807277623179 1105 100-101 1.2205153694280426 1106 1 0.1622838800861146 1106 2 0.09290082240801212 1106 3 0.07939276505729254 1106 4 0.05044049532671124 1106 5 -0.01001404357599256 1106 6 0.03758418339439373 1106 7 0.05967925189369794 1106 8 0.11932859205098367 1106 9 0.12337325598892335 1106 10-11 0.12834228388552305 1106 12-13 0.08240500944342699 1106 14-15 0.125459761671614 1106 16-17 0.15653738135289075 1106 18-19 -0.009715737336883024 1106 20-21 0.1433042144642016 1106 22-23 0.14644297355139457 1106 24-25 0.12063334091700995 1106 26-27 0.17157648678243476 1106 28-29 0.19899046002738174 1106 30-31 0.10944133608384732 1106 32-33 0.11468897157335789 1106 34-35 0.12368982388017713 1106 36-37 0.12898731973255906 1106 38-39 0.15394068246569503 1106 40-41 0.18888135951270613 1106 42-43 0.1382879322540731 1106 44-45 0.06987521335401681 1106 46-47 0.14489028838634255 1106 48-49 0.08666042636460958 1106 50-51 0.07351236910370318 1106 52-53 0.10619874038274446 1106 54-55 0.1698744424601628 1106 56-57 0.1280571222314535 1106 58-59 0.2090724279904279 1106 60-61 0.16699407662610355 1106 62-63 0.16088354745514266 1106 64-65 0.22720802086444536 1106 66-67 0.16428931160920257 1106 68-69 0.16056915774564118 1106 70-71 0.13303964991020933 1106 72-73 0.230522799634997 1106 74-75 0.37803978153513285 1106 76-77 0.3644301052428247 1106 78-79 0.23612675547111195 1106 80-81 0.2422053007065159 1106 82-83 0.3417186858345964 1106 84-85 0.3989228850317872 1106 86-87 0.5828709277150388 1106 88-89 0.44107119638163894 1106 90-91 0.3798052968154657 1106 92-93 0.5812808597707715 1106 94-95 0.7074122415383002 1106 96-97 0.8294098992287324 1106 98-99 0.8753702214063139 1106 100-101 1.4735655881431988 1107 1 0.05475350136530466 1107 2 0.03522884921347469 1107 3 -0.015707077088734422 1107 4 -0.02095503806921073 1107 5 -0.061407663215241826 1107 6 -0.04522478556363296 1107 7 0.07941072063881194 1107 8 0.12268537199818041 1107 9 0.08988758960548893 1107 10-11 0.005024217279860466 1107 12-13 0.07945163788942722 1107 14-15 0.04584951133837478 1107 16-17 0.021108304976920067 1107 18-19 0.04656788028709968 1107 20-21 0.04945722686078824 1107 22-23 0.026679484042638535 1107 24-25 0.04201380935908361 1107 26-27 0.04556388854458504 1107 28-29 0.14920476425675844 1107 30-31 0.14464683034802306 1107 32-33 0.023455976279265656 1107 34-35 0.07347613239954143 1107 36-37 0.05813868005951406 1107 38-39 0.0396898117435498 1107 40-41 -0.10413159040579245 1107 42-43 -0.008847306467892224 1107 44-45 0.05324023012970969 1107 46-47 -0.01626583293642625 1107 48-49 -4.214304559724269E-5 1107 50-51 -0.0849421492188398 1107 52-53 -0.07301944978772923 1107 54-55 -0.06334863633114907 1107 56-57 -0.05855377315909038 1107 58-59 -0.04108502986731111 1107 60-61 -0.02665261331758728 1107 62-63 0.026471572091843143 1107 64-65 0.013476773451607471 1107 66-67 -0.03135028171379162 1107 68-69 -0.04924642407104329 1107 70-71 -0.050564536933841 1107 72-73 -0.013110694547975754 1107 74-75 0.14641772545240528 1107 76-77 0.03242654498697206 1107 78-79 0.05011943306178779 1107 80-81 0.12573364906271678 1107 82-83 0.0705530446246101 1107 84-85 0.09154362314767894 1107 86-87 0.13013503863766118 1107 88-89 0.08627384724409026 1107 90-91 0.34091189070986516 1107 92-93 0.503641292938326 1107 94-95 0.4866402635408065 1107 96-97 0.7287878292817958 1107 98-99 0.7904073787374131 1107 100-101 1.3796931002892414 1108 1 0.16502120410711285 1108 2 0.08781188545253116 1108 3 0.08479487357534055 1108 4 0.04741718524191896 1108 5 0.04438985918498162 1108 6 0.048869664507741106 1108 7 0.026547329012231557 1108 8 0.09424451887731067 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-1.425699892297743 2316 96-97 -1.5083385832013327 2316 98-99 -1.6507868492645947 2316 100-101 -1.7902468740921869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 57.0 5 143.0 6 362.0 7 926.0 8 2203.0 9 4079.0 10 7027.0 11 10226.0 12 14798.0 13 19979.0 14 23779.0 15 30621.0 16 38118.0 17 50616.0 18 47325.0 19 34130.0 20 31817.0 21 33067.0 22 35621.0 23 39401.0 24 45672.0 25 53837.0 26 65174.0 27 79515.0 28 97376.0 29 117543.0 30 142781.0 31 173229.0 32 213627.0 33 269559.0 34 358676.0 35 503275.0 36 765249.0 37 1167336.0 38 957046.0 39 95059.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44948570226929 19.935372879450934 14.883852465257258 22.731288953022517 2 15.42979029088427 21.608047198929047 39.27013617329423 23.692026336892447 3 18.95865970204428 25.835814368530574 30.609385167466712 24.59614076195843 4 12.847319306426625 17.327325556790598 37.73353410698467 32.091821029798105 5 12.971540971553996 38.3265122335669 34.467475719109984 14.234471075769111 6 30.5562815140071 37.33446866201648 17.529741337135285 14.579508486841142 7 27.13650860021869 33.7810108299854 21.56612490817065 17.516355661625262 8 25.938739198861043 34.57653964948046 20.280568276726182 19.204152874932316 9 26.240713525346347 16.937618256850172 20.886144628647717 35.935523589155764 10-11 24.665336052916857 26.724592252370023 27.660727132909578 20.94934456180355 12-13 26.037694252305638 24.236120882328578 27.987080772571034 21.739104092794754 14-15 22.642883935298606 25.651739702724296 26.750346066882514 24.955030295094584 16-17 21.539064360886425 28.788573731185746 27.46551484965326 22.206847058274565 18-19 21.58402959663088 27.419429806206164 29.654224294745173 21.342316302417785 20-21 22.66901754623298 26.260119313950213 29.530962270300027 21.539900869516778 22-23 22.141706385535326 26.308371545073335 29.587036499159563 21.962885570231773 24-25 22.280999469006318 26.535676770979677 29.53294258757417 21.650381172439836 26-27 21.782392471963608 26.489856801099982 29.812337142957908 21.91541358397851 28-29 21.60958447016048 27.3198055876373 29.44349153723144 21.627118404970773 30-31 21.901575576413414 26.625293573591346 29.22284790166094 22.250282948334306 32-33 21.626819993496436 26.780259556491824 29.335963935978622 22.256956514033117 34-35 22.42006466902005 26.706894516969626 29.160799414352432 21.712241399657888 36-37 22.383813081823188 26.248201208838307 29.42020566476656 21.947780044571942 38-39 21.91512735617539 26.750218706399465 29.255052495414468 22.079601442010674 40-41 22.117662890327978 26.78986010014448 29.18416289068972 21.908314118837826 42-43 21.93813799012903 26.749700999532 28.693319346065067 22.61884166427391 44-45 22.026472073432938 26.642400388347266 28.907445951146954 22.42368158707284 46-47 22.604352816938725 27.076896727529082 28.298175140303915 22.020575315228278 48-49 22.260053814465337 27.15762230673341 28.08251491065813 22.49980896814312 50-51 22.021926742481902 27.356079471894645 28.325193779908414 22.296800005715035 52-53 22.486516322745935 27.35794799972076 27.754627276834338 22.40090840069897 54-55 22.121595260719392 27.074538484048006 28.034980335588116 22.768885919644482 56-57 22.213938945741464 27.310089157353858 27.70900668865533 22.76696520824935 58-59 22.34720484622031 26.91583815729327 27.904017854115132 22.832939142371288 60-61 21.917458638617894 27.272414055662637 28.35400354956321 22.456123756156256 62-63 21.811476121405914 27.217500840986613 28.352064124777094 22.61895891283038 64-65 21.65968614605089 27.499269333190572 27.967465794303546 22.873578726454983 66-67 21.813883791993618 27.32761014041507 27.664210209324892 23.19429585826642 68-69 21.987817908495597 27.103417380685745 27.913877868736236 22.99488684208242 70-71 22.13384814478792 26.98869932912525 27.6223087897533 23.255143736333533 72-73 21.962388217774546 27.026114440257842 27.772830376768905 23.23866696519871 74-75 21.68113292089023 27.83112891944157 27.3262229625279 23.161515197140297 76-77 21.77995827332115 27.148571985940546 27.603262129428707 23.468207611309598 78-79 21.843378866256653 27.33805542263901 27.536663535832957 23.281902175271387 80-81 22.122968792727516 27.28405468832516 27.292880519942763 23.30009599900456 82-83 22.0128066784049 27.12696870962646 27.571723665769042 23.288500946199598 84-85 21.724671828763174 27.390104096773577 27.390384422704013 23.494839651759236 86-87 21.784906653404352 27.34044963623537 27.20463705415874 23.670006656201533 88-89 21.644600699481373 27.337653810716045 26.921792813286714 24.095952676515864 90-91 21.614273371722938 27.127346132550617 27.133719081325818 24.12466141440063 92-93 21.85171533896869 27.174564322114165 27.135219071929555 23.838501266987592 94-95 21.558713523703045 26.95300825598948 27.305822290638066 24.182455929669413 96-97 21.32168501523936 26.729101097025964 27.59788706189382 24.351326825840854 98-99 21.314437436497133 27.102914702425885 26.996255207280228 24.58639265379675 100-101 21.36087437564459 26.471728969955432 27.563387305854686 24.60400934854529 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1259.0 1 916.0 2 802.0 3 1101.0 4 1002.0 5 988.0 6 1607.0 7 3186.5 8 5047.0 9 5807.0 10 5338.5 11 4844.5 12 5025.0 13 5486.0 14 6023.0 15 6628.0 16 7167.5 17 7699.0 18 8393.0 19 9217.0 20 10404.0 21 12138.0 22 14323.0 23 17500.5 24 21160.5 25 25765.5 26 32852.5 27 42090.0 28 50928.5 29 59971.5 30 69862.5 31 82619.0 32 100062.0 33 109072.0 34 110921.0 35 118871.0 36 132379.0 37 143315.0 38 150649.5 39 155732.5 40 158918.0 41 162948.5 42 164391.0 43 170039.0 44 178188.5 45 185648.5 46 192318.0 47 207079.0 48 233097.5 49 229693.0 50 203572.0 51 190362.0 52 187147.0 53 188015.5 54 184757.0 55 172173.5 56 157712.0 57 141689.0 58 129586.5 59 118375.0 60 99066.5 61 81017.5 62 66934.5 63 49760.5 64 33926.5 65 24753.5 66 17923.0 67 13794.0 68 10568.5 69 7723.5 70 5673.0 71 3878.0 72 2836.5 73 2367.0 74 1829.0 75 1322.0 76 924.0 77 570.0 78 370.0 79 252.0 80 166.5 81 132.0 82 96.5 83 62.0 84 31.5 85 14.5 86 15.0 87 12.5 88 7.5 89 3.5 90 3.5 91 4.5 92 1.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23543761048006628 2 0.0 3 0.0 4 0.0 5 0.0285209027290724 6 0.0034181677969528754 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0028756014799762282 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.9788196578287435E-4 26-27 0.0014558862838873357 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006429410856173265 36-37 0.016503058808039674 38-39 0.013518944064668116 40-41 0.008427863457037247 42-43 0.007966682087607098 44-45 0.003725622043239642 46-47 5.606518608758684E-4 48-49 0.001727169442375659 50-51 4.521385974805391E-5 52-53 0.0 54-55 0.0 56-57 0.0011755603534494014 58-59 3.70753649934042E-4 60-61 0.00265857495318557 62-63 0.0010489615461548507 64-65 0.001483014599736168 66-67 0.005660775240456349 68-69 2.5319761458910187E-4 70-71 8.138494754649703E-5 72-73 0.0 74-75 5.516090889262576E-4 76-77 0.05998974911371792 78-79 0.1420076906966877 80-81 9.766193705579643E-4 82-83 9.675765986083535E-4 84-85 0.0 86-87 0.1059360733896903 88-89 0.35491975625027355 90-91 0.39107275850481743 92-93 3.797964218836528E-4 94-95 3.5266810603482046E-4 96-97 1.898982109418264E-4 98-99 0.0 100-101 0.001365458564391228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5529278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.97756477153657 #Duplication Level Percentage of deduplicated Percentage of total 1 61.546083506246 20.296399552616258 2 15.485013548555266 10.213160745712054 3 7.192298058784168 7.11553425269255 4 4.247963272821441 5.603499359063104 5 2.648433158756816 4.3669438017994056 6 1.7782117881820583 3.518465665337022 7 1.3024168511887602 3.006537524873263 8 0.9643939596583609 2.5442691415929786 9 0.7334978244731261 2.1770074814709313 >10 3.7257060624766867 21.518981441310498 >50 0.23517023239342308 5.3330020616581315 >100 0.1277570485481711 7.759384568981037 >500 0.007624381813189236 1.701661843284708 >1k 0.0049914934883045605 3.156263574775802 >5k 2.1940630717728115E-4 0.513520509284872 >10k+ 2.1940630717728115E-4 1.175368475547304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20149 0.36440562402541526 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18714 0.3384528685300323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15580 0.28177277394987194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10535 0.19053120497829915 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9125 0.16503058808039675 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7169 0.1296552642135194 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6625 0.11981672833234285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8085543899221563E-5 2 0.0 0.0 0.0 0.0 1.8085543899221563E-5 3 0.0 0.0 0.0 0.0 1.8085543899221563E-5 4 0.0 0.0 0.0 0.0 1.8085543899221563E-5 5 0.0 0.0 0.0 0.0 1.8085543899221563E-5 6 0.0 0.0 0.0 0.0 1.8085543899221563E-5 7 0.0 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 8 0.0 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 9 0.0 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 10-11 0.0 0.0 0.0 1.8085543899221563E-5 1.8085543899221563E-5 12-13 0.0 0.0 0.0 3.617108779844313E-5 3.617108779844313E-5 14-15 0.0 0.0 0.0 6.329940364727547E-5 4.521385974805391E-5 16-17 0.0 0.0 0.0 1.898982109418264E-4 7.234217559688625E-5 18-19 0.0 0.0 0.0 3.6171087798443126E-4 7.234217559688625E-5 20-21 0.0 0.0 0.0 5.516090889262576E-4 8.138494754649704E-5 22-23 0.0 0.0 0.0 9.49491054709132E-4 9.042771949610781E-5 24-25 0.0 0.0 0.0 0.001528228459484222 9.042771949610781E-5 26-27 0.0 0.0 0.0 0.002531976145891019 1.0851326339532937E-4 28-29 0.0 0.0 0.0 0.007478372402328116 1.2659880729455093E-4 30-31 0.0 9.042771949610782E-6 0.0 0.022127662960697583 1.2659880729455093E-4 32-33 0.0 1.8085543899221563E-5 0.0 0.05271936046623085 1.3564157924416173E-4 34-35 0.0 1.8085543899221563E-5 0.0 0.09651350501819586 1.446843511937725E-4 36-37 0.0 1.8085543899221563E-5 0.0 0.16519335797548973 1.8085543899221563E-4 38-39 0.0 1.8085543899221563E-5 0.0 0.2726938309124627 1.8085543899221563E-4 40-41 0.0 1.8085543899221563E-5 0.0 0.3973213139219985 1.8085543899221563E-4 42-43 0.0 1.8085543899221563E-5 0.0 0.5228440313545457 1.8085543899221563E-4 44-45 0.0 1.8085543899221563E-5 0.0 0.6784882221512465 1.9894098289143717E-4 46-47 0.0 1.8085543899221563E-5 0.0 0.8634852506963838 2.2606929874026952E-4 48-49 0.0 1.8085543899221563E-5 0.0 1.05617767817064 2.5319761458910187E-4 50-51 0.0 1.8085543899221563E-5 0.0 1.276866527600891 2.5319761458910187E-4 52-53 0.0 1.8085543899221563E-5 0.0 1.496587800432534 2.5319761458910187E-4 54-55 0.0 1.8085543899221563E-5 0.0 1.7321158386320963 2.5319761458910187E-4 56-57 0.0 1.8085543899221563E-5 0.0 2.014349793951398 2.5319761458910187E-4 58-59 0.0 1.8085543899221563E-5 0.0 2.318756626091146 2.6224038653871267E-4 60-61 0.0 1.8085543899221563E-5 0.0 2.6566126716725043 2.89368702387545E-4 62-63 0.0 1.8085543899221563E-5 0.0 2.996349613819381 2.89368702387545E-4 64-65 0.0 1.8085543899221563E-5 0.0 3.345391568302408 2.89368702387545E-4 66-67 0.0 1.8085543899221563E-5 0.0 3.7457060397397273 3.0745424628676656E-4 68-69 0.0 1.8085543899221563E-5 0.0 4.192247161383458 3.0745424628676656E-4 70-71 0.0 1.8085543899221563E-5 0.0 4.671604502432325 3.0745424628676656E-4 72-73 0.0 1.8085543899221563E-5 0.0 5.165231337617678 3.1649701823637736E-4 74-75 0.0 1.8085543899221563E-5 0.0 5.675460702102517 3.255397901859881E-4 76-77 0.0 1.8085543899221563E-5 0.0 6.20996990927206 3.4362533408520966E-4 78-79 0.0 1.8085543899221563E-5 0.0 6.821523533452288 4.0692473773248515E-4 80-81 0.0 1.8085543899221563E-5 0.0 7.4692572882029085 4.340530535813175E-4 82-83 0.0 1.8085543899221563E-5 0.0 8.141316099497981 4.340530535813175E-4 84-85 0.0 1.8085543899221563E-5 0.0 8.81486696816474 4.340530535813175E-4 86-87 0.0 1.8085543899221563E-5 0.0 9.507687983856119 4.5213859748053904E-4 88-89 0.0 1.8085543899221563E-5 0.0 10.245234549610274 4.702241413797606E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 25850 0.0 25.455444 1 TACATGG 25695 0.0 25.248846 2 ACATGGG 25160 0.0 24.37101 3 GTATCAA 44980 0.0 21.779903 1 CATGGGG 11675 0.0 21.422758 4 GAGTACT 21845 0.0 20.775547 12-13 AGTACTT 22470 0.0 20.46163 12-13 AGAGTAC 35420 0.0 19.939754 10-11 GTACTTT 22535 0.0 19.792007 14-15 TAAGGTG 2840 0.0 19.719019 5 GGTATCA 34780 0.0 19.384712 1 CAACGCA 51815 0.0 18.749254 5 TCAACGC 51845 0.0 18.738405 4 TATCAAC 52280 0.0 18.736816 2 ATCAACG 51860 0.0 18.732985 3 CATGGGA 12300 0.0 18.63647 4 AACGCAG 52900 0.0 18.396093 6 TACTTTT 25695 0.0 17.57032 14-15 GGTAAGG 2630 0.0 17.504015 3 AGGTAAG 2390 0.0 17.474567 2 >>END_MODULE