FastQCFastQC Report
Thu 26 May 2016
SRR938815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483445
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.4134295508090964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.32950328458419426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31260.21072570941288693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.045.831851
GTATCAA58950.038.6916541
ATCAACG73550.030.6650053
TCAACGC74600.030.2333974
TATCAAC74950.030.2186492
CAACGCA76100.029.6374655
AACGCAG77400.029.1180616
ACGCAGA85750.025.8978967
CGCAGAG87450.025.2861548
GTACATG44350.022.9879841
GCAGAGT94650.022.6622669
TACATGG45750.022.3707962
GAGTACT58850.021.92531212-13
ACATGGG45800.021.4152743
AGAGTAC83800.020.31330510-11
AGTACTT62050.020.26042212-13
CATGGGG24850.020.0208114
CGCCGTA2400.019.81429794-95
CAGAGTA90450.019.65744810-11
CAATACG1250.006118783718.9530354
Produced by FastQC (version 0.11.5)