##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938815_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1483445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63360084128498 33.0 31.0 34.0 30.0 34.0 2 31.698172160073344 33.0 31.0 34.0 30.0 34.0 3 31.707527410857836 33.0 31.0 34.0 30.0 34.0 4 35.277262722918614 37.0 35.0 37.0 33.0 37.0 5 35.316238889881326 37.0 35.0 37.0 33.0 37.0 6 35.3968606857686 37.0 35.0 37.0 33.0 37.0 7 35.318395356754046 37.0 35.0 37.0 33.0 37.0 8 35.291052246628624 37.0 35.0 37.0 33.0 37.0 9 36.87564756361038 39.0 37.0 39.0 33.0 39.0 10-11 36.777830657692064 39.0 37.0 39.0 32.5 39.0 12-13 36.673606031905464 39.0 37.0 39.0 32.0 39.0 14-15 37.845745882051574 40.0 38.0 41.0 32.0 41.0 16-17 37.78169396236463 40.0 38.0 41.0 32.0 41.0 18-19 37.734314046021254 40.0 37.5 41.0 32.0 41.0 20-21 37.63825992874693 40.0 37.0 41.0 32.0 41.0 22-23 37.53942781835525 40.0 37.0 41.0 32.0 41.0 24-25 37.531894003485135 40.0 37.0 41.0 32.0 41.0 26-27 37.44094253578663 40.0 37.0 41.0 32.0 41.0 28-29 37.31782978135354 40.0 37.0 41.0 31.0 41.0 30-31 37.10861912642531 40.0 36.5 41.0 31.0 41.0 32-33 36.88824931156868 39.5 36.0 41.0 30.5 41.0 34-35 36.559155546717264 39.0 35.5 41.0 30.0 41.0 36-37 36.30895988728938 39.0 35.0 41.0 30.0 41.0 38-39 36.13392036779254 39.0 35.0 41.0 29.5 41.0 40-41 35.94892901320912 39.0 35.0 41.0 29.0 41.0 42-43 35.68375403199984 39.0 35.0 40.5 27.5 41.0 44-45 35.629129492498876 39.0 35.0 41.0 27.0 41.0 46-47 35.42668046338085 39.0 35.0 41.0 26.0 41.0 48-49 35.184843725247646 39.0 35.0 41.0 24.5 41.0 50-51 34.03612705560368 38.0 33.5 40.0 23.5 40.5 52-53 34.13048141319698 38.0 33.5 39.5 23.5 40.5 54-55 34.661543569191984 38.5 34.0 40.0 24.0 41.0 56-57 34.58409883750325 38.0 34.0 40.0 23.0 41.0 58-59 34.38080919750985 38.0 34.0 40.0 23.0 41.0 60-61 34.111018608711476 38.0 34.0 40.0 22.5 41.0 62-63 33.7620413968836 37.5 33.5 40.0 21.5 41.0 64-65 33.38618890488019 37.0 33.0 40.0 20.5 41.0 66-67 33.0050001853793 36.0 33.0 39.5 18.5 41.0 68-69 32.60256666071206 36.0 32.0 39.0 18.0 41.0 70-71 32.2152536831497 35.0 32.0 39.0 14.5 40.0 72-73 31.802260616335623 35.0 31.5 38.0 12.0 40.0 74-75 31.351309283458434 35.0 31.0 37.0 7.5 39.5 76-77 30.915269524653763 35.0 31.0 37.0 6.5 39.0 78-79 30.484434542568145 35.0 31.0 36.0 2.0 39.0 80-81 30.048538031406625 34.5 30.0 36.0 2.0 37.5 82-83 29.583750324413778 34.0 30.0 35.0 2.0 37.0 84-85 29.16799915062574 34.0 29.0 35.0 2.0 37.0 86-87 28.79209306715112 34.0 29.0 35.0 2.0 36.0 88-89 28.420708553401035 34.0 29.0 35.0 2.0 36.0 90-91 28.011653280033975 34.0 28.5 35.0 2.0 35.5 92-93 27.560584989669316 34.0 27.0 35.0 2.0 35.0 94-95 27.071008025238548 34.0 26.0 35.0 2.0 35.0 96-97 26.500444573273697 33.5 25.0 35.0 2.0 35.0 98-99 25.822097887013 33.0 23.5 35.0 2.0 35.0 100-101 23.712604444384525 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4716004414808985 1101 2 0.3874099226081533 1101 3 0.3901913206608434 1101 4 0.39548086468684573 1101 5 0.23340268198647607 1101 6 0.0817303945069483 1101 7 0.2632938547513959 1101 8 0.4611097813281191 1101 9 0.4838893475801527 1101 10-11 0.36770101805528554 1101 12-13 0.3026154050203118 1101 14-15 0.548039705216901 1101 16-17 0.5766750019048388 1101 18-19 0.5836754494454865 1101 20-21 0.5081392910630527 1101 22-23 0.5519039174730693 1101 24-25 0.6077045831359982 1101 26-27 0.662266519382591 1101 28-29 0.6534676548304432 1101 30-31 0.49274960443999305 1101 32-33 0.3712757650608225 1101 34-35 0.508384648520348 1101 36-37 0.7067660919226455 1101 38-39 0.6684531810665604 1101 40-41 0.7383610271688497 1101 42-43 0.80540840848888 1101 44-45 1.0369115023699607 1101 46-47 0.8182100711241631 1101 48-49 0.9325097986927133 1101 50-51 0.7866586075647533 1101 52-53 0.7554482569742547 1101 54-55 0.7913923843589572 1101 56-57 0.8068236780696552 1101 58-59 0.7254303185009405 1101 60-61 0.5187953200419955 1101 62-63 0.5662381841931037 1101 64-65 0.6490262830627103 1101 66-67 0.3376136763532074 1101 68-69 0.31579507526969763 1101 70-71 0.14720263583733129 1101 72-73 0.28645939055556724 1101 74-75 0.195177053823965 1101 76-77 0.23099312367322256 1101 78-79 0.15439122247711623 1101 80-81 0.10405568043038826 1101 82-83 -0.09682779311375356 1101 84-85 -0.12470478774605809 1101 86-87 -0.18399542093245458 1101 88-89 -0.2783193081128559 1101 90-91 -0.2372394230717596 1101 92-93 -0.3105434900006081 1101 94-95 -0.6294984231692737 1101 96-97 -0.8079503565382744 1101 98-99 -0.8660170022174718 1101 100-101 -0.4545849486219673 1102 1 0.4157466277530695 1102 2 0.28763052900363917 1102 3 0.26011988521228346 1102 4 0.26095910335199335 1102 5 0.290270050240089 1102 6 0.2687047798858089 1102 7 0.29849198189938164 1102 8 0.22140073479147304 1102 9 0.398371403949902 1102 10-11 0.31767632018558345 1102 12-13 0.2694145611066645 1102 14-15 0.16693770929414598 1102 16-17 0.32539170687412167 1102 18-19 0.3595830961571025 1102 20-21 0.32638102250930245 1102 22-23 0.4295547523866432 1102 24-25 0.4263497480047391 1102 26-27 0.403128830852026 1102 28-29 0.2735204936203317 1102 30-31 0.43870810155369355 1102 32-33 0.4736602083331647 1102 34-35 0.29653893276352505 1102 36-37 0.3681632692491661 1102 38-39 0.35759857198360834 1102 40-41 0.23883326927495574 1102 42-43 0.280407832902938 1102 44-45 0.25879831496336436 1102 46-47 0.20445231117908236 1102 48-49 0.17495287847520302 1102 50-51 0.2911453000176678 1102 52-53 0.13852529758341348 1102 54-55 0.03705246632239323 1102 56-57 0.1305711507240943 1102 58-59 0.008857210713571817 1102 60-61 0.08348751760821216 1102 62-63 0.1673225881162992 1102 64-65 0.055453799001206505 1102 66-67 -0.10712065410429261 1102 68-69 -0.21005815240697956 1102 70-71 -0.22561458527557932 1102 72-73 -0.2842181159642543 1102 74-75 -0.46477566440682594 1102 76-77 -0.5444200423843704 1102 78-79 -0.6557025666365632 1102 80-81 -0.6890164624656272 1102 82-83 -0.7706607664442409 1102 84-85 -0.7905080754929834 1102 86-87 -0.6924876683130137 1102 88-89 -0.8213807241798676 1102 90-91 -0.6481609571024975 1102 92-93 -0.912347376356923 1102 94-95 -0.9980217731213408 1102 96-97 -1.035528667622593 1102 98-99 -0.926622801193556 1102 100-101 -0.811066255683464 1103 1 0.11744877361433126 1103 2 0.18693753934617874 1103 3 0.09740053339750787 1103 4 0.12159330200765339 1103 5 0.05846457177213438 1103 6 0.1733683266302748 1103 7 0.07041402198012037 1103 8 0.12665074857705605 1103 9 0.03442583097726981 1103 10-11 0.16438991452251628 1103 12-13 0.22790243468951843 1103 14-15 0.22885971330046573 1103 16-17 0.21572571995265122 1103 18-19 0.2883948613662142 1103 20-21 0.1784617179004897 1103 22-23 0.3421559604675295 1103 24-25 0.24710264181761943 1103 26-27 0.29922135246962966 1103 28-29 0.3052263036122227 1103 30-31 0.20628910022769986 1103 32-33 0.101201844658668 1103 34-35 0.32568268573000125 1103 36-37 0.13567572652030435 1103 38-39 0.008475979242710707 1103 40-41 0.06181015123893019 1103 42-43 0.16793809032485285 1103 44-45 0.20455861691658583 1103 46-47 0.3748171215074976 1103 48-49 0.23892107526426543 1103 50-51 0.31748272058307947 1103 52-53 0.37336566568681917 1103 54-55 0.44535901439697057 1103 56-57 0.1843411082541948 1103 58-59 0.20152065232686311 1103 60-61 0.079180489228456 1103 62-63 0.1758683611473657 1103 64-65 -0.034214847120559 1103 66-67 -0.013784527579950634 1103 68-69 -0.17550246303250105 1103 70-71 -0.1926930672432654 1103 72-73 -0.24386423618587472 1103 74-75 -0.43922956206781194 1103 76-77 -0.42035108457959325 1103 78-79 -0.3764352587728048 1103 80-81 -0.4779024997573025 1103 82-83 -0.3183277046997759 1103 84-85 -0.275239060793691 1103 86-87 -0.3796121400159791 1103 88-89 -0.26006734721709535 1103 90-91 -0.009357916750161621 1103 92-93 -0.057027455493898316 1103 94-95 -0.09020867161255453 1103 96-97 -0.12827420431466052 1103 98-99 -0.014809046222371336 1103 100-101 -0.40810129889289115 1104 1 0.19533358885421492 1104 2 0.281608459396427 1104 3 0.28109579593550293 1104 4 0.2174119126694265 1104 5 0.19527170251548398 1104 6 0.23118964234760853 1104 7 0.32147059017433577 1104 8 0.28045635207856634 1104 9 0.24950937743162882 1104 10-11 0.27547341277989545 1104 12-13 0.31491078782879356 1104 14-15 0.3479996381734196 1104 16-17 0.3704199552924834 1104 18-19 0.4727784370399988 1104 20-21 0.4960564776837231 1104 22-23 0.3169489393723097 1104 24-25 0.4844953658808606 1104 26-27 0.5156303159031808 1104 28-29 0.5128460957024998 1104 30-31 0.5990297311864339 1104 32-33 0.4544179388355687 1104 34-35 0.49543320610678165 1104 36-37 0.49662668307976077 1104 38-39 0.6279487645161197 1104 40-41 0.2915049137332417 1104 42-43 0.49799478202383796 1104 44-45 0.5693841589067006 1104 46-47 0.4113858278101574 1104 48-49 0.5158247807084209 1104 50-51 0.6660093991182521 1104 52-53 0.49790892398348063 1104 54-55 0.578492631490036 1104 56-57 0.3653689691215547 1104 58-59 0.43571806716128947 1104 60-61 0.42134064779652647 1104 62-63 0.27674614866494807 1104 64-65 0.22249884172575918 1104 66-67 0.23280930139907952 1104 68-69 0.13627025205406085 1104 70-71 0.2316783386927952 1104 72-73 0.2487397864546601 1104 74-75 0.12811216356484678 1104 76-77 -0.03960623036039834 1104 78-79 0.14826494094343445 1104 80-81 0.037510744170628385 1104 82-83 0.11177010504866658 1104 84-85 0.0644238916931279 1104 86-87 0.15744154840861313 1104 88-89 0.038750169975671156 1104 90-91 0.003904536897898936 1104 92-93 -0.14408174638075266 1104 94-95 -0.15185185731251138 1104 96-97 0.0903405240802897 1104 98-99 0.28333672554684597 1104 100-101 0.5195580448449633 1105 1 0.21514781321524268 1105 2 0.2045868356177749 1105 3 0.1560350494350935 1105 4 0.2692528773436109 1105 5 0.1582469574532368 1105 6 0.1656891109391978 1105 7 0.10532663973557277 1105 8 0.1539534935359299 1105 9 0.10895975494890564 1105 10-11 0.12041377255721386 1105 12-13 0.11134136297468444 1105 14-15 0.156700018653936 1105 16-17 0.23990930934481725 1105 18-19 0.25540523424743355 1105 20-21 0.30224847218394046 1105 22-23 0.40002693985433524 1105 24-25 0.265550656061464 1105 26-27 0.26506334856603075 1105 28-29 0.2824094320780901 1105 30-31 0.2402263285922075 1105 32-33 0.25027759565241325 1105 34-35 0.4522834358569625 1105 36-37 0.28356500019427244 1105 38-39 0.47861213605554553 1105 40-41 0.42861519777004986 1105 42-43 0.36639465006069827 1105 44-45 0.5938358600062585 1105 46-47 0.5290759989276168 1105 48-49 0.5111092718799242 1105 50-51 0.43821150420673405 1105 52-53 0.5123833827963651 1105 54-55 0.49875865177443046 1105 56-57 0.22748182130309402 1105 58-59 0.2557903900679577 1105 60-61 0.35873866281819744 1105 62-63 0.3129890912056723 1105 64-65 0.15250109379113752 1105 66-67 0.1472031599772805 1105 68-69 0.09474285785174885 1105 70-71 0.22517154891322377 1105 72-73 0.2447502723871544 1105 74-75 0.1247469062169877 1105 76-77 0.19013407094224277 1105 78-79 0.29967682789765604 1105 80-81 0.09771694643771056 1105 82-83 0.1402493485127394 1105 84-85 -0.07880746653935944 1105 86-87 -0.19255509324827713 1105 88-89 -0.15406169169219908 1105 90-91 -0.01235725187417458 1105 92-93 -0.0635150951229555 1105 94-95 0.2124256445543331 1105 96-97 0.14372533883803484 1105 98-99 0.36399869448415245 1105 100-101 0.4757366059727346 1106 1 0.07924894871389654 1106 2 0.15826249262969583 1106 3 0.08082690083935873 1106 4 0.18895578004541846 1106 5 -0.00855638234100553 1106 6 0.08326932956389044 1106 7 0.02802915792160121 1106 8 0.19868288074332696 1106 9 0.15905863043641233 1106 10-11 0.22115592725812405 1106 12-13 0.29326637454939686 1106 14-15 0.38647076092635047 1106 16-17 0.28772070827601937 1106 18-19 0.34117775784806526 1106 20-21 0.42317083923172305 1106 22-23 0.36750102427516396 1106 24-25 0.2690426871680103 1106 26-27 0.14749274283596492 1106 28-29 0.2779482642487281 1106 30-31 0.2105666250617233 1106 32-33 0.3559056511796328 1106 34-35 0.30622629210298413 1106 36-37 0.49956772319380605 1106 38-39 0.5276790967329177 1106 40-41 0.5116951299697092 1106 42-43 0.625833924032122 1106 44-45 0.30237779969221634 1106 46-47 0.5505892647221913 1106 48-49 0.2835024118044345 1106 50-51 0.5070918177832837 1106 52-53 0.4627043357677252 1106 54-55 0.3482560347129109 1106 56-57 0.37301712220631345 1106 58-59 0.5484912603291932 1106 60-61 0.5759282840309154 1106 62-63 0.49052257939163013 1106 64-65 0.4579674957435387 1106 66-67 0.27517138641784555 1106 68-69 0.4487975375657882 1106 70-71 0.23982728957693666 1106 72-73 0.28863313632349374 1106 74-75 0.0667144499458523 1106 76-77 -0.060096442347091994 1106 78-79 0.07448201964547607 1106 80-81 -0.07896217121627203 1106 82-83 -0.004357032879834577 1106 84-85 -0.0061683149852065355 1106 86-87 0.17909706906446843 1106 88-89 0.1565260289139232 1106 90-91 0.10292293457674617 1106 92-93 0.20703884302493591 1106 94-95 0.1729215522077645 1106 96-97 0.05720045909730587 1106 98-99 0.09887604179607123 1106 100-101 0.22812511601330598 1107 1 0.15431100577041335 1107 2 0.10230321644881357 1107 3 -0.024813995660263544 1107 4 0.09658969737462542 1107 5 0.04390431485653323 1107 6 0.09371110385608006 1107 7 -0.08489669738652594 1107 8 0.08179976386021082 1107 9 0.18821645322304903 1107 10-11 0.07949945353793453 1107 12-13 0.061843019511087505 1107 14-15 0.2959843775036788 1107 16-17 0.3027088364912984 1107 18-19 0.32046059412536465 1107 20-21 0.26156167277490994 1107 22-23 0.18905550857203224 1107 24-25 -0.009885794322798347 1107 26-27 0.12447317237871403 1107 28-29 -0.03445954954413111 1107 30-31 0.03199069960214018 1107 32-33 0.09325590480411705 1107 34-35 0.1259370522569654 1107 36-37 0.15319941006649174 1107 38-39 0.1548870688162438 1107 40-41 0.3107247015062171 1107 42-43 0.27276524755624365 1107 44-45 0.40160163679693284 1107 46-47 0.4923006004778543 1107 48-49 0.5669367752249528 1107 50-51 0.7072033406094889 1107 52-53 0.6254736993681149 1107 54-55 0.6470922400588108 1107 56-57 0.555442383925211 1107 58-59 0.5661685276601958 1107 60-61 0.44095868299483243 1107 62-63 0.37456582146811 1107 64-65 0.4068108961104784 1107 66-67 0.30679800080068986 1107 68-69 0.3434399450198313 1107 70-71 0.35247433781955806 1107 72-73 0.46326978776069794 1107 74-75 0.566588537077557 1107 76-77 0.6156194622469293 1107 78-79 0.5405934705214719 1107 80-81 0.378596198391957 1107 82-83 0.5147817231010272 1107 84-85 0.5790432186335259 1107 86-87 0.6039450742448835 1107 88-89 0.45443587530022356 1107 90-91 0.4888323852368863 1107 92-93 0.540438130717849 1107 94-95 0.5189076298420439 1107 96-97 0.5216615520210048 1107 98-99 0.7136186399181703 1107 100-101 0.7452897380948222 1108 1 -0.1693072899382706 1108 2 -0.26386946473413886 1108 3 -0.24177034100791062 1108 4 -0.21762941212676878 1108 5 -0.15109729046596243 1108 6 -0.2723797012798528 1108 7 -0.12132417866772727 1108 8 -0.1647796112875568 1108 9 -0.3449566851778343 1108 10-11 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70-71 -1.7276749738996635 2316 72-73 -1.8891149859026548 2316 74-75 -1.8128178547410911 2316 76-77 -1.7713178555493059 2316 78-79 -1.6534702911619732 2316 80-81 -1.471647613771534 2316 82-83 -1.4247619375284053 2316 84-85 -1.4556505694871866 2316 86-87 -1.3702522706029008 2316 88-89 -1.3234198062098415 2316 90-91 -1.7308096453743218 2316 92-93 -2.1146784816487845 2316 94-95 -2.0024822266485884 2316 96-97 -1.8934821118949898 2316 98-99 -2.091852980017343 2316 100-101 -2.438313947708121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6013.0 3 1439.0 4 1339.0 5 1534.0 6 1677.0 7 2047.0 8 2601.0 9 3114.0 10 4013.0 11 5375.0 12 7056.0 13 9202.0 14 9863.0 15 9577.0 16 11486.0 17 14606.0 18 12306.0 19 9047.0 20 8926.0 21 9609.0 22 10804.0 23 12103.0 24 13755.0 25 15968.0 26 18698.0 27 22000.0 28 26163.0 29 30798.0 30 37298.0 31 45033.0 32 55250.0 33 69236.0 34 91302.0 35 126420.0 36 184024.0 37 278258.0 38 267380.0 39 48118.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64041408516132 19.715026116641727 14.428701838157846 23.215857960039106 2 15.66976057520249 21.358764739698742 39.59894794514632 23.372526739952445 3 18.108331123692007 25.439280068495506 31.44032855173698 25.012060256075504 4 12.789297063048997 17.719552658695427 38.17496462338157 31.316185654874012 5 13.277213481993345 37.10517949960549 35.123053528267924 14.49455349013324 6 30.096718764330454 36.95517299049941 18.42424348689286 14.523864758277268 7 26.35210730940812 33.61639853926485 22.54664270323675 17.48485144809029 8 24.930483382442898 34.720945493441526 21.49353325163768 18.855037872477894 9 25.952123706278517 16.78455667825334 21.766691080959685 35.49662853450846 10-11 23.821798237405645 26.68689924009826 28.889720672463298 20.601581850032794 12-13 24.877816839070285 24.849962484298203 28.722864344908405 21.54935633172311 14-15 22.320389226928093 26.210938711866056 27.152408995814486 24.316263065391365 16-17 21.312815394186885 28.922336104615187 27.909938678855582 21.854909822342343 18-19 21.1172575473696 27.61023455825918 30.36587932032195 20.906628574049268 20-21 22.003319733800787 27.0266934385684 30.209914821377144 20.760072006253672 22-23 21.748984795542544 26.93226124145002 29.98623908908166 21.332514873925774 24-25 21.548344745720062 27.076754724033208 30.16442405031053 21.210476479936204 26-27 21.411099795337805 27.16748127865465 30.03892528564831 21.382493640359236 28-29 21.35378193153983 27.300484163332435 30.070037393302933 21.275696511824798 30-31 21.601850883790263 27.147622147200572 29.86337590680827 21.387151062200893 32-33 21.312890109804876 27.291406649668115 29.803014887613642 21.592688352913363 34-35 21.78521637654644 27.236008172857833 29.61653556829353 21.36223988230219 36-37 21.891903698719346 27.037417950443306 29.504416105841603 21.56626224499575 38-39 21.515491519547563 27.25618360680603 29.607369407533003 21.6209554661134 40-41 21.75651519905658 27.064668129588764 29.63591788545814 21.54289878589651 42-43 21.675798865672874 27.176753844377576 29.345423974316304 21.802023315633242 44-45 21.632478499346124 27.250313731094018 29.309019540384796 21.808188229175062 46-47 21.75991423476675 27.291345120409265 28.911057850221017 22.03768279460297 48-49 21.710862983909287 27.423335343924553 28.72824404264982 22.137557629516337 50-51 21.637757220250226 27.630481941501124 28.57504231270153 22.15671852554712 52-53 21.728548002879975 27.58545950592014 28.544311470935064 22.14168102026482 54-55 21.599459821333333 27.51264181298481 28.572647630606106 22.315250735075754 56-57 21.543646655742588 27.685833457470583 28.505714827404088 22.264805059382745 58-59 21.623228965056903 27.32953418455387 28.572144224136238 22.475092626252984 60-61 21.55477629652389 27.308475925215948 28.80179505547624 22.334952722783918 62-63 21.476398267554018 27.159361465265757 28.886965754667663 22.47727451251256 64-65 21.400050195821283 27.17002936895859 28.79597430868127 22.633946126538856 66-67 21.365155923465913 27.31551512154477 28.53580634126019 22.783522613729122 68-69 21.404730990184166 27.145047859686112 28.669403506085732 22.78081764404399 70-71 21.38799829955304 26.98155421529623 28.638846405942758 22.99160107920797 72-73 21.474105272427675 26.917713683789824 28.609435940164644 22.998745103617864 74-75 21.381942250070868 27.15737544904927 28.398439762286266 23.062242538593598 76-77 21.43197937142441 26.98977544583221 28.53133142691704 23.04691375582634 78-79 21.376568737372917 27.059297866463005 28.54171067526798 23.022422720896095 80-81 21.413012788264073 26.941988664101384 28.435250568589225 23.20974797904531 82-83 21.479920959644183 26.900401838135213 28.494921833205346 23.12475536901526 84-85 21.420672777407592 26.75186085062814 28.466917759611686 23.36054861235258 86-87 21.455438423498528 26.578201337409403 28.64147431981612 23.32488591927595 88-89 21.60237115385136 26.459247147815105 28.528080237765614 23.41030146056792 90-91 21.594488268978328 26.40878604009098 28.656605312319233 23.34012037861146 92-93 21.73429609735116 26.239259502609556 28.596021824758434 23.430422575280854 94-95 21.67701947502117 26.0702116850127 28.60961896697714 23.643149872988992 96-97 21.78670872849561 25.780268039163023 28.586181307301423 23.84684192503994 98-99 21.854968308290985 25.73922202484874 28.51295355213003 23.89285611473025 100-101 22.177672921365932 25.34898864997445 28.737707976849563 23.735630451810056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1378.0 1 879.5 2 472.0 3 573.5 4 512.5 5 531.5 6 781.5 7 1301.0 8 1975.5 9 2280.5 10 2066.0 11 1917.5 12 2032.0 13 2202.0 14 2346.0 15 2447.5 16 2588.5 17 2746.5 18 2968.0 19 3211.5 20 3430.5 21 3884.0 22 4552.0 23 5272.5 24 6143.5 25 7447.0 26 9515.0 27 12114.0 28 14406.0 29 16655.5 30 19404.0 31 22468.0 32 25810.5 33 28406.0 34 31303.5 35 34785.0 36 38327.0 37 41683.5 38 43327.5 39 44011.5 40 45052.0 41 46742.5 42 48445.5 43 50174.0 44 52265.0 45 54080.0 46 54828.5 47 55385.0 48 56438.0 49 55717.5 50 53694.5 51 51763.0 52 49668.5 53 48070.0 54 46011.0 55 42293.5 56 37912.5 57 33558.0 58 30004.0 59 26512.0 60 22107.0 61 17860.0 62 14261.5 63 10968.5 64 8019.5 65 5903.5 66 4409.5 67 3466.5 68 2610.0 69 1905.0 70 1427.0 71 1014.0 72 825.0 73 684.5 74 491.0 75 367.5 76 288.5 77 200.5 78 135.5 79 97.5 80 68.0 81 53.5 82 41.0 83 34.5 84 23.0 85 17.5 86 17.0 87 15.5 88 13.5 89 8.0 90 4.5 91 5.0 92 4.0 93 2.0 94 3.0 95 2.5 96 2.5 97 1.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16158334147878756 2 0.09390304325404716 3 0.08790349490543971 4 0.10340794569397584 5 0.12497935548672179 6 0.03970487614977299 7 0.042468713029468565 8 0.04759192285524573 9 0.04765933351084806 10-11 0.046344825726602604 12-13 0.049378305228707495 14-15 0.04792897613325738 16-17 0.04567071917057929 18-19 0.050086117112531976 20-21 0.053658881859455526 22-23 0.0663994957682961 24-25 0.08160059860662175 26-27 0.08402738220830566 28-29 0.07374725722895017 30-31 0.061343696598121264 32-33 0.051872499485993755 34-35 0.29680911661706366 36-37 0.47015561749845797 38-39 0.44740452123267127 40-41 0.4222940520208029 42-43 0.45235920441944255 44-45 0.46257191874319575 46-47 0.4621674548095817 48-49 0.4783797174819424 50-51 0.46769512856897294 52-53 0.47484065806282 54-55 0.46489758636147616 56-57 0.44389916714135 58-59 0.4227996319378204 60-61 0.39303782748939126 62-63 0.4509098753239925 64-65 0.48714310271024536 66-67 0.4880531465608769 68-69 0.4996140739966766 70-71 0.4963109518721624 72-73 0.48721051336584775 74-75 0.48073909042802393 76-77 0.4619315175149736 78-79 0.43554024584666096 80-81 0.44113533026165447 82-83 0.4537411228592904 84-85 0.4517188031912205 86-87 0.4623696867763888 88-89 0.47409914085119437 90-91 0.4788178867433575 92-93 0.48131208100064377 94-95 0.48501966705877203 96-97 0.4857274789425965 98-99 0.4794582879715797 100-101 0.4670547273407507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1483445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.82348050486144 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71030950661817 55.26990754866554 2 10.754070672822458 14.37248863906507 3 2.8864451099029087 5.7864692558984885 4 1.181218512015069 3.1573252883848157 5 0.6525211724454733 2.1801867922959706 6 0.38869468042835253 1.5584358839968417 7 0.25143379268493515 1.1761176800621598 8 0.1853740846243108 0.990987322399933 9 0.14622369156960027 0.8794058402655051 >10 0.7799082721579796 9.402137701885573 >50 0.0459625505452744 2.11513168721927 >100 0.017031765368024088 1.8785377964933463 >500 4.0309440875133714E-4 0.19885628510073658 >1k 3.0232080656350285E-4 0.6210134308157781 >5k 1.0077360218783428E-4 0.41299884745100485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6133 0.4134295508090964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4888 0.32950328458419426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3126 0.21072570941288693 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.741065560233106E-5 0.0 3 0.0 0.0 0.0 6.741065560233106E-5 0.0 4 0.0 0.0 0.0 6.741065560233106E-5 0.0 5 0.0 0.0 0.0 6.741065560233106E-5 0.0 6 0.0 0.0 0.0 6.741065560233106E-5 0.0 7 0.0 0.0 0.0 1.348213112046621E-4 0.0 8 0.0 0.0 0.0 1.348213112046621E-4 0.0 9 0.0 0.0 0.0 2.696426224093242E-4 0.0 10-11 0.0 0.0 0.0 2.696426224093242E-4 6.741065560233106E-5 12-13 0.0 0.0 0.0 2.696426224093242E-4 6.741065560233106E-5 14-15 0.0 0.0 0.0 2.696426224093242E-4 6.741065560233106E-5 16-17 0.0 0.0 0.0 3.370532780116553E-4 6.741065560233106E-5 18-19 0.0 0.0 0.0 4.3816926141515187E-4 6.741065560233106E-5 20-21 0.0 0.0 0.0 6.066959004209795E-4 1.6852663900582764E-4 22-23 0.0 0.0 0.0 0.001078570489637297 2.0223196680699317E-4 24-25 0.0 1.348213112046621E-4 0.0 0.0023930782738827523 2.0223196680699317E-4 26-27 0.0 1.348213112046621E-4 0.0 0.004718745892163174 2.0223196680699317E-4 28-29 0.0 1.348213112046621E-4 0.0 0.014223648332091854 2.0223196680699317E-4 30-31 0.0 1.348213112046621E-4 0.0 0.04213165975145691 2.0223196680699317E-4 32-33 0.0 1.348213112046621E-4 0.0 0.098958842424222 2.0223196680699317E-4 34-35 0.0 1.348213112046621E-4 0.0 0.1886150143753223 2.0223196680699317E-4 36-37 0.0 1.348213112046621E-4 0.0 0.3307503817128373 2.0223196680699317E-4 38-39 0.0 1.348213112046621E-4 0.0 0.5279265493496557 2.0223196680699317E-4 40-41 0.0 1.348213112046621E-4 0.0 0.7483930984970795 2.0223196680699317E-4 42-43 0.0 1.348213112046621E-4 0.0 0.9685562996942927 2.0223196680699317E-4 44-45 0.0 1.348213112046621E-4 0.0 1.235165442601512 2.0223196680699317E-4 46-47 0.0 1.348213112046621E-4 0.0 1.5512540067208422 2.0223196680699317E-4 48-49 0.0 1.348213112046621E-4 0.0 1.8690615425580321 2.0223196680699317E-4 50-51 0.0 1.348213112046621E-4 0.0 2.216765704154856 2.0223196680699317E-4 52-53 0.0 1.348213112046621E-4 0.0 2.557695094863645 2.0223196680699317E-4 54-55 0.0 1.348213112046621E-4 0.0 2.933307267879834 2.0223196680699317E-4 56-57 0.0 1.348213112046621E-4 0.0 3.3673307739754423 2.0223196680699317E-4 58-59 0.0 1.348213112046621E-4 0.0 3.8279140783783694 2.0223196680699317E-4 60-61 0.0 1.348213112046621E-4 0.0 4.326618108524414 2.0223196680699317E-4 62-63 0.0 1.348213112046621E-4 0.0 4.827411868994131 2.0223196680699317E-4 64-65 0.0 1.348213112046621E-4 0.0 5.349945565895601 2.0223196680699317E-4 66-67 0.0 1.348213112046621E-4 0.0 5.938575410615156 2.0223196680699317E-4 68-69 0.0 1.348213112046621E-4 0.0 6.589796049061475 2.0223196680699317E-4 70-71 0.0 1.348213112046621E-4 0.0 7.299259493948208 2.0223196680699317E-4 72-73 0.0 1.348213112046621E-4 0.0 8.02399145232887 2.0223196680699317E-4 74-75 0.0 1.348213112046621E-4 0.0 8.765609779937915 2.0223196680699317E-4 76-77 0.0 1.348213112046621E-4 0.0 9.549494588609623 2.0223196680699317E-4 78-79 0.0 1.348213112046621E-4 0.0 10.396307244286104 2.0223196680699317E-4 80-81 0.0 1.348213112046621E-4 0.0 11.31781090636997 2.0223196680699317E-4 82-83 0.0 1.348213112046621E-4 0.0 12.251549602445658 2.0223196680699317E-4 84-85 0.0 1.348213112046621E-4 0.0 13.196040298089919 2.696426224093242E-4 86-87 0.0 1.348213112046621E-4 0.0 14.174539669485554 2.696426224093242E-4 88-89 0.0 1.348213112046621E-4 0.0 15.196316681777889 2.696426224093242E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3435 0.0 45.83185 1 GTATCAA 5895 0.0 38.691654 1 ATCAACG 7355 0.0 30.665005 3 TCAACGC 7460 0.0 30.233397 4 TATCAAC 7495 0.0 30.218649 2 CAACGCA 7610 0.0 29.637465 5 AACGCAG 7740 0.0 29.118061 6 ACGCAGA 8575 0.0 25.897896 7 CGCAGAG 8745 0.0 25.286154 8 GTACATG 4435 0.0 22.987984 1 GCAGAGT 9465 0.0 22.662266 9 TACATGG 4575 0.0 22.370796 2 GAGTACT 5885 0.0 21.925312 12-13 ACATGGG 4580 0.0 21.415274 3 AGAGTAC 8380 0.0 20.313305 10-11 AGTACTT 6205 0.0 20.260422 12-13 CATGGGG 2485 0.0 20.020811 4 CGCCGTA 240 0.0 19.814297 94-95 CAGAGTA 9045 0.0 19.657448 10-11 CAATACG 125 0.0061187837 18.953035 4 >>END_MODULE