FastQCFastQC Report
Thu 26 May 2016
SRR938815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483445
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.38821796561382454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50040.3373229206340646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.21524222333824308No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT19810.13354050874821785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.048.6968231
GTATCAA59850.036.5323451
ATCAACG75200.028.9635563
TATCAAC76300.028.4216082
TCAACGC77400.028.14034
CAACGCA78100.027.8880835
AACGCAG80650.027.123996
TGGACCG1901.2043529E-824.9748785
ACGCAGA92050.023.66177
CGCAGAG92650.023.2523828
GAGTACT59300.021.9264112-13
GCAGAGT97300.021.5071459
CTAGACG1150.003745532820.6314224
GTACATG47650.020.4936811
AGAGTAC86500.020.0509810-11
CAGAGTA94000.019.66273710-11
AGTACTT63950.019.55292512-13
GTACTTT65550.019.54621714-15
ACATGGG47350.019.2414683
TACATGG49500.019.0767732
Produced by FastQC (version 0.11.5)