##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938815_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1483445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94839781724297 31.0 31.0 34.0 30.0 34.0 2 32.17336537586496 34.0 31.0 34.0 30.0 34.0 3 32.25956877403611 34.0 31.0 34.0 30.0 34.0 4 35.91327147282171 37.0 35.0 37.0 35.0 37.0 5 35.814162304635495 37.0 35.0 37.0 35.0 37.0 6 35.83454391635686 37.0 35.0 37.0 35.0 37.0 7 35.7633703979588 37.0 35.0 37.0 33.0 37.0 8 35.735944372727 37.0 35.0 37.0 33.0 37.0 9 37.380381476900055 39.0 37.0 39.0 34.0 39.0 10-11 37.309020556879425 39.0 37.0 39.0 34.0 39.0 12-13 37.24253511252524 39.0 37.0 39.0 33.0 39.0 14-15 38.463597908921464 40.0 38.0 41.0 33.5 41.0 16-17 38.4004337875688 40.0 38.0 41.0 33.0 41.0 18-19 38.42234562117234 40.0 38.0 41.0 33.5 41.0 20-21 38.477109026623836 40.0 38.0 41.0 34.0 41.0 22-23 38.420801580105774 40.0 38.0 41.0 34.0 41.0 24-25 38.35206967565363 40.0 38.0 41.0 34.0 41.0 26-27 38.18166632399583 40.0 38.0 41.0 33.0 41.0 28-29 38.006513891650854 40.0 37.0 41.0 33.0 41.0 30-31 37.76799375777329 40.0 37.0 41.0 32.5 41.0 32-33 37.52822147096792 40.0 36.5 41.0 32.0 41.0 34-35 37.47090657220187 40.0 36.0 41.0 32.0 41.0 36-37 37.369592738524176 40.0 36.0 41.0 31.5 41.0 38-39 37.250614616652456 40.0 36.0 41.0 31.0 41.0 40-41 37.08532773375487 40.0 36.0 41.0 31.0 41.0 42-43 36.90101891205943 40.0 36.0 41.0 31.0 41.0 44-45 36.634606608266566 39.5 35.5 41.0 30.0 41.0 46-47 36.32792048239065 39.0 35.0 41.0 30.0 41.0 48-49 36.02774218120658 39.0 35.0 41.0 29.0 41.0 50-51 35.78706187287024 39.0 35.0 41.0 28.0 41.0 52-53 35.581564533905876 39.0 35.0 40.5 28.0 41.0 54-55 35.339335128703794 38.5 35.0 40.0 27.0 41.0 56-57 35.086984350616305 38.0 34.0 40.0 26.5 41.0 58-59 34.782345823404306 38.0 34.0 40.0 26.0 41.0 60-61 34.43849687720138 38.0 34.0 40.0 26.0 41.0 62-63 34.06716865134872 37.0 33.5 40.0 25.0 41.0 64-65 33.6986787511502 36.5 33.0 39.5 23.5 41.0 66-67 33.30389802116021 36.0 33.0 39.0 23.0 40.5 68-69 32.85357967433912 35.5 32.0 39.0 22.0 40.0 70-71 32.38981762047127 35.0 32.0 38.0 21.0 40.0 72-73 32.0549504700208 35.0 31.5 37.5 20.0 40.0 74-75 31.633962162399012 35.0 31.0 37.0 18.5 39.0 76-77 29.550508781923156 33.0 28.5 35.0 13.0 37.5 78-79 30.614853263855416 34.0 30.5 36.0 14.5 38.0 80-81 30.66810902999437 34.0 31.0 36.0 14.0 37.5 82-83 30.35831291352224 34.0 31.0 35.0 9.5 37.0 84-85 29.983075880804478 34.0 30.0 35.0 7.0 36.5 86-87 29.461117533848576 34.0 29.5 35.0 2.0 36.0 88-89 28.946357633751166 34.0 29.0 35.0 2.0 36.0 90-91 28.54913191928248 34.0 29.0 35.0 2.0 35.0 92-93 28.27363367027426 34.0 29.0 35.0 2.0 35.0 94-95 27.896236126044442 34.0 28.5 35.0 2.0 35.0 96-97 27.404375962708425 33.0 27.0 35.0 2.0 35.0 98-99 26.70244936617131 33.0 25.5 35.0 2.0 35.0 100-101 23.764596934837492 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1015872569922287 1101 2 0.07492481581463295 1101 3 0.14940598588810872 1101 4 0.08310538164597148 1101 5 0.0667437719620878 1101 6 0.13114852275214872 1101 7 0.02403131426565608 1101 8 0.07116677196679433 1101 9 0.07755743797693526 1101 10-11 0.1668817943024692 1101 12-13 0.14327007784286394 1101 14-15 0.20592242148170925 1101 16-17 0.06033527980256537 1101 18-19 0.15844808889333706 1101 20-21 0.03605069049358889 1101 22-23 0.13627536579209476 1101 24-25 0.17205934708272963 1101 26-27 0.12075290319669563 1101 28-29 0.13812351521702482 1101 30-31 0.2130760625089252 1101 32-33 0.2578070035251727 1101 34-35 0.24917224115848313 1101 36-37 0.1963797633639146 1101 38-39 0.20007299938831835 1101 40-41 0.2663851424944994 1101 42-43 0.3540712582298724 1101 44-45 0.5492091320801507 1101 46-47 0.571525848150948 1101 48-49 0.5318130538617609 1101 50-51 0.36982423544500875 1101 52-53 0.4059021603162023 1101 54-55 0.4138280724704657 1101 56-57 0.28734810002030287 1101 58-59 0.5124493677894719 1101 60-61 0.4876028531814782 1101 62-63 0.2551645109361971 1101 64-65 0.3871309084259664 1101 66-67 0.20613953654948602 1101 68-69 0.2677105594446658 1101 70-71 0.3539017480504185 1101 72-73 0.43662237757538236 1101 74-75 0.4211181428157609 1101 76-77 0.24807257063697818 1101 78-79 0.40034073277529814 1101 80-81 0.43940045762860436 1101 82-83 0.4685938354313137 1101 84-85 0.4926129230466181 1101 86-87 0.6515445247984744 1101 88-89 0.8605606562270474 1101 90-91 0.7203002432830523 1101 92-93 0.6286561017481311 1101 94-95 0.6266292174590191 1101 96-97 0.5702989106232543 1101 98-99 0.5590118527847032 1101 100-101 1.0016341165998845 1102 1 -0.022960123149847078 1102 2 -0.032229926804255626 1102 3 0.11914533964113616 1102 4 0.10366688143457736 1102 5 0.04171258566898928 1102 6 0.04704599729031855 1102 7 -0.031178366462050633 1102 8 0.005161715182623539 1102 9 0.1604925704872855 1102 10-11 0.05160448745865409 1102 12-13 -0.02485543756818487 1102 14-15 0.20002057249034522 1102 16-17 0.1338509482985515 1102 18-19 0.0906971752814627 1102 20-21 0.10015709854426547 1102 22-23 0.13153044455318508 1102 24-25 0.15861575241244452 1102 26-27 0.12256730707803598 1102 28-29 0.039163808326321714 1102 30-31 0.08907234945630904 1102 32-33 0.14283658864282245 1102 34-35 0.1840156503854331 1102 36-37 0.2561598581367619 1102 38-39 0.06453140440826388 1102 40-41 0.16601080232529597 1102 42-43 0.1469408995247079 1102 44-45 0.034782070331289106 1102 46-47 0.03171047519218462 1102 48-49 -0.024499920473381565 1102 50-51 0.03834716678911576 1102 52-53 0.0032245868191083105 1102 54-55 0.1360591901118653 1102 56-57 -0.05624426791936088 1102 58-59 -0.007805240037654926 1102 60-61 -0.0957623990088905 1102 62-63 -0.2702347887326795 1102 64-65 -0.13887223322678466 1102 66-67 0.016687776930915277 1102 68-69 -0.040200189151953225 1102 70-71 -0.012088762107111961 1102 72-73 0.15625263162855418 1102 74-75 0.1375855520929541 1102 76-77 0.02645392876864605 1102 78-79 -0.057657190929216284 1102 80-81 0.03000893569564056 1102 82-83 0.20506442612900955 1102 84-85 0.1785604107709382 1102 86-87 0.12269589553024218 1102 88-89 0.22241218016933217 1102 90-91 0.32656492072468524 1102 92-93 0.16940967979968136 1102 94-95 0.2964898628687749 1102 96-97 0.3302261984204158 1102 98-99 0.31498643490930434 1102 100-101 0.91129119295816 1103 1 0.03431369150904473 1103 2 0.04931475935599394 1103 3 -0.008900823251373424 1103 4 0.05488395514957034 1103 5 -0.020948686325461097 1103 6 -0.008975004207798065 1103 7 0.1055663704085319 1103 8 0.07582429410186364 1103 9 0.18753653438606221 1103 10-11 -0.02608487362725498 1103 12-13 0.0870451021567149 1103 14-15 0.042380241698197096 1103 16-17 -0.0846281231252064 1103 18-19 0.016778106702439288 1103 20-21 0.10575327218179353 1103 22-23 0.09890782887925553 1103 24-25 0.09337722060021036 1103 26-27 0.08630065937620657 1103 28-29 0.07563298814340413 1103 30-31 -0.011667536950483282 1103 32-33 0.03213268904927702 1103 34-35 0.028286490965868438 1103 36-37 0.09988932470427159 1103 38-39 0.23830730906458797 1103 40-41 0.12024472846446344 1103 42-43 0.16730619943457015 1103 44-45 0.1678693761027361 1103 46-47 0.1273198056869802 1103 48-49 0.17846895296877818 1103 50-51 0.06212227265466197 1103 52-53 0.08128628748076494 1103 54-55 -0.029790648558169153 1103 56-57 0.06762988800357306 1103 58-59 0.09147646667419451 1103 60-61 0.11593416562384817 1103 62-63 0.09491180483689732 1103 64-65 0.14899495930189488 1103 66-67 -0.1021434323919479 1103 68-69 -0.09278639027304081 1103 70-71 -0.2627305003170832 1103 72-73 -0.15506370859035457 1103 74-75 -0.1028136268531874 1103 76-77 -0.39074290307249626 1103 78-79 -0.431423252421677 1103 80-81 -0.09854098074053397 1103 82-83 -0.2575217595316168 1103 84-85 -0.40661645439266536 1103 86-87 -0.07414084142485677 1103 88-89 0.152066283145313 1103 90-91 0.14738712896377493 1103 92-93 0.060387878995424416 1103 94-95 0.039086747172433434 1103 96-97 0.16621229018446826 1103 98-99 0.2145573038854529 1103 100-101 0.9699345473022696 1104 1 0.037755257187971125 1104 2 0.05713321337758259 1104 3 -0.009543951717411403 1104 4 0.04989255529837777 1104 5 0.14059253263643967 1104 6 0.09639112717114529 1104 7 0.05985002771595305 1104 8 0.05854718254693836 1104 9 0.020946681657960653 1104 10-11 0.13369037782543103 1104 12-13 0.06375993568132543 1104 14-15 -0.07272092677736453 1104 16-17 0.11992965451199922 1104 18-19 0.16774761909961455 1104 20-21 0.11265673664103559 1104 22-23 0.18370740474582448 1104 24-25 0.0918273864279584 1104 26-27 0.048548114726223446 1104 28-29 0.04227917109312074 1104 30-31 0.1056627418991738 1104 32-33 0.12783532572537126 1104 34-35 0.13567087092675933 1104 36-37 0.02801407054050742 1104 38-39 0.33454839638848455 1104 40-41 0.36388875158696976 1104 42-43 0.3169954795222978 1104 44-45 0.2669337522078834 1104 46-47 0.22922177905211782 1104 48-49 0.18264100414192086 1104 50-51 0.3912172839658936 1104 52-53 0.3862157843344107 1104 54-55 0.2559180428636765 1104 56-57 0.27632507523378536 1104 58-59 0.31045794397191173 1104 60-61 0.4034773546373458 1104 62-63 0.32046614542689156 1104 64-65 0.36676247513912585 1104 66-67 0.2938053101186924 1104 68-69 0.056606177150015924 1104 70-71 0.05553073334489511 1104 72-73 0.08991552152311755 1104 74-75 0.194100109587648 1104 76-77 0.19508161171945915 1104 78-79 0.3883817907899285 1104 80-81 0.31453350468138197 1104 82-83 0.2898091820265343 1104 84-85 0.3990533179669704 1104 86-87 0.5058488263473251 1104 88-89 0.8177562078676566 1104 90-91 0.761186978950672 1104 92-93 0.840736162429824 1104 94-95 0.7605081966363905 1104 96-97 0.7035845305307653 1104 98-99 0.45039040376466133 1104 100-101 1.0238820306667797 1105 1 0.02366700783297304 1105 2 0.008582437205220117 1105 3 0.03423402624395777 1105 4 0.031817755119789126 1105 5 -0.04614616061250132 1105 6 0.02829578183470005 1105 7 0.04379714764040443 1105 8 -0.022127133240040564 1105 9 0.06873787963821343 1105 10-11 0.04219034424200174 1105 12-13 0.03667539235672024 1105 14-15 -0.0020920078872777026 1105 16-17 -0.09525568651786642 1105 18-19 -0.0471300384363218 1105 20-21 0.05238830625159352 1105 22-23 -0.05422626600222458 1105 24-25 -0.037007051207616826 1105 26-27 -0.09161332280267231 1105 28-29 -0.1525709085193725 1105 30-31 -0.3077127485176945 1105 32-33 -0.10733907105693419 1105 34-35 -0.10435040516459537 1105 36-37 -0.12748067266478103 1105 38-39 -0.12200550896945828 1105 40-41 -0.007543008856494282 1105 42-43 -0.027617441801602638 1105 44-45 0.007172826727533277 1105 46-47 0.0754852272407831 1105 48-49 -0.13837708744997457 1105 50-51 -0.14767924350733352 1105 52-53 -0.11698748234569223 1105 54-55 -0.16617333567690906 1105 56-57 -0.2641424653922897 1105 58-59 -0.2787006330388522 1105 60-61 -0.39761242770251215 1105 62-63 -0.4338616723217541 1105 64-65 -0.3620231677465213 1105 66-67 -0.3066259406468106 1105 68-69 -0.2601334840919378 1105 70-71 -0.3770302798474745 1105 72-73 -0.6386503474382828 1105 74-75 -0.6509044538079927 1105 76-77 -0.66079855074533 1105 78-79 -0.6958108832808989 1105 80-81 -0.6510429845462369 1105 82-83 -0.5759657457690928 1105 84-85 -0.5418131740658545 1105 86-87 -0.26119278945031965 1105 88-89 -0.21551657651486167 1105 90-91 -0.1113184635406661 1105 92-93 -0.03854276179410121 1105 94-95 -0.2124961573566786 1105 96-97 -0.4885388707991396 1105 98-99 -0.43603685254803537 1105 100-101 -0.05164562430099906 1106 1 0.021793474289857784 1106 2 -0.06438728021490903 1106 3 -0.060543229255010544 1106 4 0.009890546312028903 1106 5 -0.04617558784092779 1106 6 -0.1033851799255956 1106 7 -0.0013226407974400445 1106 8 -0.06418256629113017 1106 9 0.049818133575577406 1106 10-11 -0.026062188461459357 1106 12-13 -0.006045096056794819 1106 14-15 -1.409826426410632E-4 1106 16-17 -0.07226321204107933 1106 18-19 -0.16236216041414053 1106 20-21 -0.12113228888467376 1106 22-23 0.04182322667236349 1106 24-25 5.450349867004434E-4 1106 26-27 -0.09379388042903258 1106 28-29 0.014681686962184415 1106 30-31 -0.037347144631731055 1106 32-33 0.05095489518414098 1106 34-35 -0.064558906887072 1106 36-37 -0.015974037805676744 1106 38-39 -0.028882063708017824 1106 40-41 -0.0010817704017753726 1106 42-43 0.16624423453027504 1106 44-45 0.26163988000259053 1106 46-47 0.12250307191210652 1106 48-49 0.1735682092941957 1106 50-51 0.2746603388614446 1106 52-53 0.12410391470216808 1106 54-55 0.1562872906914592 1106 56-57 0.1831621966654211 1106 58-59 0.14689458170144576 1106 60-61 0.2145573007037882 1106 62-63 0.05331394153023439 1106 64-65 0.2153665193831884 1106 66-67 0.20485067843929983 1106 68-69 0.13128396664687614 1106 70-71 0.01614397642838128 1106 72-73 0.07264499992072615 1106 74-75 0.05677526808507949 1106 76-77 -0.04380569960001779 1106 78-79 0.18061928002060057 1106 80-81 0.1699521658465173 1106 82-83 0.316777892064124 1106 84-85 0.17514985748798395 1106 86-87 0.3111128535150023 1106 88-89 0.4779429276951923 1106 90-91 0.7249170484538716 1106 92-93 0.6950996228315418 1106 94-95 0.7992207840949099 1106 96-97 0.9747310435872443 1106 98-99 0.8028945859333625 1106 100-101 1.1734177423308765 1107 1 -0.16466549610263215 1107 2 -0.16308498140568872 1107 3 -0.2553843991654361 1107 4 -0.12598742613104008 1107 5 -0.11648893100745994 1107 6 -0.13360426831643224 1107 7 -0.12823381826262903 1107 8 -0.09718972533707415 1107 9 -0.13113664571714878 1107 10-11 -0.048182381288022214 1107 12-13 -0.11639423445302555 1107 14-15 -0.25191725346323324 1107 16-17 -0.17742294150828997 1107 18-19 -0.1469603184887518 1107 20-21 -0.1731702785957978 1107 22-23 -0.21650051329957165 1107 24-25 -0.11798394296056358 1107 26-27 -0.21793947167989103 1107 28-29 -0.09953027835781825 1107 30-31 -0.29735721766119383 1107 32-33 -0.1659749893357727 1107 34-35 -0.23871888818001707 1107 36-37 -0.19762133787403258 1107 38-39 -0.19193922815022546 1107 40-41 -0.1518669270733568 1107 42-43 -0.18866816608849746 1107 44-45 -0.1916914147414488 1107 46-47 -0.19128078965569273 1107 48-49 -0.2251124037215888 1107 50-51 -0.17658439977942209 1107 52-53 -0.29626518987745953 1107 54-55 -0.2173996179677502 1107 56-57 0.02603193080938837 1107 58-59 -0.04356661914756188 1107 60-61 -0.04971582229509863 1107 62-63 -0.0027577568544927544 1107 64-65 -0.00933848475394683 1107 66-67 0.23522266102734335 1107 68-69 0.3556292197317461 1107 70-71 0.06398187260422361 1107 72-73 0.2562709307765658 1107 74-75 0.1332583417980686 1107 76-77 -0.014805267898754693 1107 78-79 0.15053060138621532 1107 80-81 0.10585352210798504 1107 82-83 0.13569719990309892 1107 84-85 -0.0172270177143794 1107 86-87 0.07244051477341173 1107 88-89 0.2690534791935555 1107 90-91 0.4766798286100027 1107 92-93 0.5210385730779699 1107 94-95 0.5711116356256518 1107 96-97 0.49699063994656356 1107 98-99 0.4878111236892373 1107 100-101 0.7468114538824828 1108 1 -0.19592834673220239 1108 2 -0.1123641502074193 1108 3 -0.18904824187510627 1108 4 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1326.0 10 2125.0 11 3132.0 12 4407.0 13 6155.0 14 6999.0 15 8827.0 16 10884.0 17 14246.0 18 13396.0 19 9380.0 20 8723.0 21 9025.0 22 9722.0 23 10750.0 24 12621.0 25 14661.0 26 17388.0 27 21251.0 28 25478.0 29 31202.0 30 37663.0 31 46424.0 32 56507.0 33 70979.0 34 91741.0 35 127898.0 36 190451.0 37 305454.0 38 282326.0 39 31039.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.145210188669026 20.602076171399183 14.961359391495005 23.29135424843678 2 15.772340733899807 21.947224197728936 38.994030786446416 23.286404281924845 3 17.962243291797133 25.66573078206472 31.770844217345434 24.60118170879271 4 13.050298460677679 18.47126115225034 37.49710976814105 30.981330618930937 5 13.152155961292802 37.4003754773517 35.169554651503766 14.27791390985173 6 30.0607032953699 37.632874828524145 17.91963975745646 14.386782118649494 7 26.29595300129091 33.87682050901786 22.150265092403156 17.676961397288068 8 24.963783625277646 35.33565450690791 21.078570489637297 18.62199137817715 9 25.598185305151183 16.880976375935745 21.472316129010512 36.04852218990256 10-11 23.817431721432207 26.96958768272501 28.69540158212809 20.5175790137147 12-13 24.80503153133416 24.985793204331806 28.571871555736816 21.63730370859722 14-15 22.13346464960027 26.41597258806633 26.947574272392945 24.502988489940453 16-17 21.255553083773197 29.287014379032094 27.697669558652027 21.759762978542682 18-19 21.080912189661674 27.771300722003527 30.15012725476535 20.99765983356944 20-21 21.840655312686117 27.153324576686224 30.1163609423878 20.889659168239863 22-23 21.67650300482997 27.10211703163919 29.90201861208201 21.319361351448823 24-25 21.558568062853695 27.220557553532487 30.05517562161051 21.16569876200331 26-27 21.505260731109498 27.345386816084734 29.969820229142456 21.179532223663315 28-29 21.31686715719154 27.440215174812682 29.895985358405603 21.346932309590176 30-31 21.640337188099323 27.273171570230105 29.780713137325616 21.305778104344956 32-33 21.466653633939917 27.296461951740714 29.78411737543353 21.45276703888584 34-35 21.712034975536337 27.355986307765168 29.655823329046786 21.276155387651706 36-37 21.92027745761392 27.155509962873726 29.43250708021911 21.491705499293243 38-39 21.584336573681114 27.381129755264862 29.51545800278707 21.51907566826695 40-41 21.7263565089271 27.164999067969465 29.623436067930026 21.48520835517341 42-43 21.64198850408505 27.293511883033293 29.331840812621575 21.73265880026008 44-45 21.6242488630986 27.398076604166867 29.208355298371735 21.7693192343628 46-47 21.825728266929303 27.430651757864773 28.695129292532997 22.048490682672934 48-49 21.72977939503594 27.45574738775019 28.7137663146591 22.100706902554766 50-51 21.63607395370832 27.65424184236453 28.514269015663913 22.195415188263237 52-53 21.741419466175017 27.713835025902544 28.46323928423366 22.08150622368878 54-55 21.54282767477055 27.597652760971926 28.466542406358176 22.39297715789935 56-57 21.53618772519372 27.83483715270873 28.239772960911928 22.38920216118562 58-59 21.49864032066052 27.588075807684582 28.389491168488334 22.523792703166563 60-61 21.587037935226753 27.63653853737588 28.430640610501364 22.345782916896002 62-63 21.340764234602563 27.67965782351216 28.470283697744105 22.50929424414117 64-65 21.20860564429419 27.578474429453063 28.34473135168476 22.86818857456798 66-67 21.205850917353114 27.662022797909298 28.120252752834574 23.011873531903017 68-69 21.30537525898497 27.53233962649539 28.08561612554102 23.076668988978625 70-71 21.282990554914768 27.30456102363664 28.051316656071755 23.36113176537683 72-73 21.3447077579553 27.249139671507876 27.94859263403766 23.457559936499162 74-75 21.307800424013024 27.35251391187405 27.711745295578872 23.627940368534052 76-77 21.24056675069771 27.15095628046575 28.055347127379903 23.553129841456634 78-79 21.1975769689319 27.161725118278873 27.8917296498298 23.748968262959426 80-81 21.199579761831334 27.11823731861893 27.77269388912089 23.909489030428844 82-83 21.18973066072554 27.086578875522854 27.79614343639299 23.927547027358614 84-85 21.181439150086455 26.82944092972776 27.70433012346262 24.284789796723167 86-87 21.11263648629171 26.78225012379843 27.813751802967584 24.29136158694228 88-89 21.133482904429478 26.618470283311552 27.741098857252027 24.506947955006943 90-91 21.056413358861832 26.516627602208338 27.753621841397734 24.673337197532096 92-93 21.12754433093239 26.401888846569975 27.674602024341986 24.795964798155644 94-95 20.973255478467674 26.18976516808184 27.7135166544878 25.123462698962683 96-97 21.0901988277287 25.972651497022138 27.703993070184602 25.23315660506456 98-99 20.895842841070188 25.931027927635814 27.613299079639837 25.55983015165416 100-101 20.97346970142291 25.675998155633273 27.918326102373136 25.432206040570676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 826.0 1 572.0 2 379.5 3 456.0 4 421.5 5 425.5 6 638.5 7 1125.5 8 1720.0 9 1986.0 10 1720.5 11 1448.5 12 1514.5 13 1673.0 14 1813.0 15 1943.5 16 2074.5 17 2208.0 18 2406.5 19 2641.5 20 2991.5 21 3555.5 22 4132.5 23 4809.5 24 5746.5 25 7141.5 26 9344.0 27 11908.0 28 14226.0 29 16561.0 30 19255.5 31 22482.5 32 25926.5 33 28720.0 34 31438.5 35 34361.0 36 37820.0 37 41155.0 38 42491.5 39 43120.5 40 44424.5 41 46277.5 42 48098.5 43 49866.0 44 52322.0 45 54256.0 46 54772.0 47 55508.5 48 56852.5 49 56567.5 50 54566.0 51 52978.0 52 51567.5 53 49988.5 54 47732.5 55 43844.5 56 39408.0 57 34796.0 58 31228.0 59 27521.0 60 22571.5 61 18208.0 62 14529.5 63 10956.5 64 7880.5 65 5708.0 66 4136.5 67 3215.0 68 2375.5 69 1682.5 70 1279.0 71 884.5 72 675.5 73 598.5 74 453.0 75 328.5 76 216.5 77 129.0 78 86.5 79 68.0 80 42.0 81 25.5 82 16.5 83 10.0 84 5.0 85 4.5 86 4.5 87 4.0 88 3.0 89 1.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3859260033233453 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3593729460815872E-4 16-17 0.002359372946081587 18-19 2.3593729460815872E-4 20-21 3.3705327801165535E-5 22-23 0.0 24-25 0.0 26-27 6.741065560233107E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008257805311285555 36-37 0.017762707751214236 38-39 0.011324990141191617 40-41 0.008392626622490218 42-43 0.0032694167967130565 44-45 0.0018200877012629387 46-47 0.0017526770456606075 48-49 1.0111598340349658E-4 50-51 2.3593729460815872E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.7187458921631745E-4 60-61 5.72990572619814E-4 62-63 0.0 64-65 0.0 66-67 0.0013145077842454558 68-69 6.404012282221451E-4 70-71 0.0018874983568652698 72-73 0.0 74-75 0.0 76-77 0.09794768259018703 78-79 0.03431202370158651 80-81 3.3705327801165535E-5 82-83 0.0 84-85 0.0 86-87 0.1487416115865435 88-89 0.32171735386212497 90-91 0.27318168182844665 92-93 0.0 94-95 6.741065560233107E-5 96-97 0.0 98-99 2.696426224093243E-4 100-101 6.066959004209795E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1483445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.80568234768604 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21945914072936 54.105076110133886 2 11.006947626163061 14.48639398209807 3 3.0035011448413207 5.929423268050179 4 1.247595634485753 3.2839552808531716 5 0.6191349234274294 2.0371298050712174 6 0.404531490762503 1.59722824684519 7 0.2742713153820511 1.263402773997929 8 0.19795289421571446 1.0421140221251524 9 0.15561889556805972 0.9216546848144519 >10 0.8028449977981167 9.643820181431337 >50 0.047374909823849315 2.116270234592441 >100 0.01964179057105138 2.1109024377317835 >500 5.114710144023618E-4 0.23398832416969112 >1k 4.091768115218894E-4 0.5041250171768747 >5k 2.045884057609447E-4 0.7245156309086265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5759 0.38821796561382454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5004 0.3373229206340646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3193 0.21524222333824308 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1981 0.13354050874821785 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.741065560233106E-5 2 0.0 0.0 0.0 0.0 6.741065560233106E-5 3 0.0 0.0 0.0 0.0 6.741065560233106E-5 4 0.0 0.0 0.0 0.0 6.741065560233106E-5 5 0.0 0.0 0.0 0.0 6.741065560233106E-5 6 0.0 0.0 0.0 0.0 6.741065560233106E-5 7 0.0 0.0 0.0 0.0 6.741065560233106E-5 8 0.0 0.0 0.0 0.0 6.741065560233106E-5 9 0.0 0.0 0.0 1.348213112046621E-4 6.741065560233106E-5 10-11 0.0 0.0 0.0 2.0223196680699317E-4 6.741065560233106E-5 12-13 0.0 0.0 0.0 2.696426224093242E-4 6.741065560233106E-5 14-15 0.0 0.0 0.0 2.696426224093242E-4 6.741065560233106E-5 16-17 0.0 0.0 0.0 3.0334795021048975E-4 6.741065560233106E-5 18-19 0.0 0.0 0.0 3.370532780116553E-4 6.741065560233106E-5 20-21 0.0 0.0 0.0 6.066959004209795E-4 6.741065560233106E-5 22-23 0.0 0.0 0.0 0.0010448651618361314 6.741065560233106E-5 24-25 0.0 0.0 0.0 0.0023256676182804217 6.741065560233106E-5 26-27 0.0 0.0 0.0 0.004685040564362009 1.348213112046621E-4 28-29 0.0 0.0 0.0 0.014526996282302342 1.348213112046621E-4 30-31 0.0 3.370532780116553E-5 0.0 0.04327764089669654 1.348213112046621E-4 32-33 0.0 6.741065560233106E-5 0.0 0.10074522479768377 1.348213112046621E-4 34-35 0.0 6.741065560233106E-5 0.0 0.19208666313884237 2.0223196680699317E-4 36-37 0.0 6.741065560233106E-5 0.0 0.3370532780116553 2.0223196680699317E-4 38-39 0.0 6.741065560233106E-5 0.0 0.5368247558891633 2.0223196680699317E-4 40-41 0.0 6.741065560233106E-5 0.0 0.7607966591279083 2.0223196680699317E-4 42-43 0.0 6.741065560233106E-5 0.0 0.9837236972048171 2.0223196680699317E-4 44-45 0.0 6.741065560233106E-5 0.0 1.2534674355975448 2.0223196680699317E-4 46-47 0.0 6.741065560233106E-5 0.0 1.5725220685633778 2.0223196680699317E-4 48-49 0.0 6.741065560233106E-5 0.0 1.8929586199690585 2.0223196680699317E-4 50-51 0.0 6.741065560233106E-5 0.0 2.2447074209020217 2.0223196680699317E-4 52-53 0.0 6.741065560233106E-5 0.0 2.5881310058680977 2.0223196680699317E-4 54-55 0.0 6.741065560233106E-5 0.0 2.967922639531631 2.0223196680699317E-4 56-57 0.0 6.741065560233106E-5 0.0 3.405316678407356 2.0223196680699317E-4 58-59 0.0 6.741065560233106E-5 0.0 3.8695064528850076 2.0223196680699317E-4 60-61 0.0 6.741065560233106E-5 0.0 4.373097755562222 2.0223196680699317E-4 62-63 0.0 6.741065560233106E-5 0.0 4.878981020529915 2.696426224093242E-4 64-65 0.0 6.741065560233106E-5 0.0 5.404817839555898 2.696426224093242E-4 66-67 0.0 6.741065560233106E-5 0.0 5.997660850250599 2.696426224093242E-4 68-69 0.0 6.741065560233106E-5 0.0 6.653667645244684 2.696426224093242E-4 70-71 0.0 1.0111598340349658E-4 0.0 7.368793585202013 2.696426224093242E-4 72-73 0.0 1.348213112046621E-4 0.0 8.101075537010136 3.370532780116553E-4 74-75 0.0 1.348213112046621E-4 0.0 8.853917738776968 3.370532780116553E-4 76-77 0.0 1.348213112046621E-4 0.0 9.644813255631318 3.370532780116553E-4 78-79 0.0 1.348213112046621E-4 0.0 10.498501798179237 3.370532780116553E-4 80-81 0.0 1.348213112046621E-4 0.0 11.426982463117945 3.370532780116553E-4 82-83 0.0 1.348213112046621E-4 0.0 12.370900168189586 3.370532780116553E-4 84-85 0.0 1.348213112046621E-4 0.0 13.322536393327693 3.370532780116553E-4 86-87 0.0 1.348213112046621E-4 0.0 14.308720579461996 3.370532780116553E-4 88-89 0.0 1.348213112046621E-4 0.0 15.338114995837392 3.370532780116553E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3590 0.0 48.696823 1 GTATCAA 5985 0.0 36.532345 1 ATCAACG 7520 0.0 28.963556 3 TATCAAC 7630 0.0 28.421608 2 TCAACGC 7740 0.0 28.1403 4 CAACGCA 7810 0.0 27.888083 5 AACGCAG 8065 0.0 27.12399 6 TGGACCG 190 1.2043529E-8 24.974878 5 ACGCAGA 9205 0.0 23.6617 7 CGCAGAG 9265 0.0 23.252382 8 GAGTACT 5930 0.0 21.92641 12-13 GCAGAGT 9730 0.0 21.507145 9 CTAGACG 115 0.0037455328 20.631422 4 GTACATG 4765 0.0 20.493681 1 AGAGTAC 8650 0.0 20.05098 10-11 CAGAGTA 9400 0.0 19.662737 10-11 AGTACTT 6395 0.0 19.552925 12-13 GTACTTT 6555 0.0 19.546217 14-15 ACATGGG 4735 0.0 19.241468 3 TACATGG 4950 0.0 19.076773 2 >>END_MODULE