FastQCFastQC Report
Thu 26 May 2016
SRR938814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484164
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.4165981657013646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.32422292954147924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.20846752784732686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.050.8254551
GTATCAA59500.039.5361331
ATCAACG73200.031.5638243
TATCAAC74100.031.3725322
TCAACGC74250.031.1174644
CAACGCA76100.030.2986565
AACGCAG76900.029.982446
ACGCAGA85450.026.7057487
CGCAGAG85550.026.3972478
GCAGAGT90200.023.5636849
GAGTACT54450.023.4081812-13
GTACATG45750.022.4371991
TACATGG47350.021.3418142
CAGAGTA88000.020.84702110-11
GTACTTT62450.020.82760614-15
AGAGTAC79100.020.7323610-11
ACATGGG47500.020.575263
AGTACTT58150.019.79626312-13
GTGTACG1707.9259815E-519.568391
CATGGGG24350.019.0940844
Produced by FastQC (version 0.11.5)