##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938814_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1484164 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58303664554591 33.0 31.0 34.0 30.0 34.0 2 31.651601844540092 33.0 31.0 34.0 30.0 34.0 3 31.663297991327106 33.0 31.0 34.0 28.0 34.0 4 35.22612730129554 37.0 35.0 37.0 33.0 37.0 5 35.2569527356815 37.0 35.0 37.0 33.0 37.0 6 35.3177660959301 37.0 35.0 37.0 33.0 37.0 7 35.230009621578205 37.0 35.0 37.0 32.0 37.0 8 35.20284483385933 37.0 35.0 37.0 32.0 37.0 9 36.79065116793023 39.0 37.0 39.0 33.0 39.0 10-11 36.68235383690751 39.0 37.0 39.0 32.0 39.0 12-13 36.585615201554546 39.0 37.0 39.0 32.0 39.0 14-15 37.75704504353966 40.0 38.0 41.0 32.0 41.0 16-17 37.65070133758803 40.0 37.0 41.0 32.0 41.0 18-19 37.60651349850825 40.0 37.0 41.0 32.0 41.0 20-21 37.50406996800892 40.0 37.0 41.0 32.0 41.0 22-23 37.48864141698626 40.0 37.0 41.0 32.0 41.0 24-25 37.41824555776855 40.0 37.0 41.0 32.0 41.0 26-27 37.29271630358909 40.0 37.0 41.0 31.0 41.0 28-29 37.12185479502266 40.0 37.0 41.0 31.0 41.0 30-31 36.888148816438076 39.5 36.0 41.0 30.0 41.0 32-33 36.68631600011859 39.0 36.0 41.0 30.0 41.0 34-35 36.39404809711056 39.0 35.0 41.0 30.0 41.0 36-37 36.17977022754898 39.0 35.0 41.0 29.5 41.0 38-39 36.0088655970634 39.0 35.0 41.0 29.0 41.0 40-41 35.89381732746516 39.0 35.0 41.0 28.5 41.0 42-43 35.874136887837196 39.0 35.0 41.0 28.0 41.0 44-45 35.697499737225804 39.0 35.0 41.0 27.0 41.0 46-47 35.38438912411297 39.0 35.0 41.0 26.0 41.0 48-49 35.09262386097493 39.0 35.0 41.0 24.0 41.0 50-51 33.95227009953078 38.0 33.5 40.0 23.0 40.5 52-53 34.01584764217432 38.0 33.5 39.5 23.0 40.5 54-55 34.51843933689268 38.0 34.0 40.0 23.0 41.0 56-57 34.42500121280398 38.0 34.0 40.0 23.0 41.0 58-59 34.230539212647656 38.0 34.0 40.0 22.5 41.0 60-61 33.94427401554006 38.0 34.0 40.0 22.0 41.0 62-63 33.5609235232764 37.0 33.0 40.0 20.5 41.0 64-65 33.17884243250746 37.0 33.0 40.0 19.0 41.0 66-67 32.810260860659604 36.0 32.5 39.0 17.5 41.0 68-69 32.39681126883552 36.0 32.0 39.0 14.0 41.0 70-71 31.98125645144337 35.0 31.5 39.0 11.5 40.0 72-73 31.560202578690763 35.0 31.0 38.0 7.5 40.0 74-75 31.084536816686025 35.0 31.0 37.0 6.5 39.0 76-77 30.614770335353775 35.0 30.5 37.0 2.0 39.0 78-79 30.1689510054145 34.5 30.0 36.0 2.0 39.0 80-81 29.692809891629228 34.0 29.5 36.0 2.0 37.5 82-83 29.2701655612183 34.0 29.0 35.0 2.0 37.0 84-85 28.89702856288119 34.0 29.0 35.0 2.0 37.0 86-87 28.456749051991558 34.0 29.0 35.0 2.0 36.0 88-89 27.993424918000976 34.0 27.5 35.0 2.0 36.0 90-91 27.526868324524784 34.0 26.5 35.0 2.0 35.5 92-93 27.052909247226047 34.0 25.5 35.0 2.0 35.0 94-95 26.521866181904425 33.0 25.0 35.0 2.0 35.0 96-97 25.922218164569415 33.0 23.5 35.0 2.0 35.0 98-99 25.154977819162845 33.0 18.0 35.0 2.0 35.0 100-101 22.80578157130883 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2152070889401223 1101 2 0.28147880672694825 1101 3 0.25593891066974095 1101 4 0.11070329065458395 1101 5 0.1552448608058441 1101 6 0.19516653446346766 1101 7 0.24220679723021732 1101 8 0.2811392951078773 1101 9 0.2660255385966934 1101 10-11 0.3474691866438562 1101 12-13 0.23593649049703203 1101 14-15 0.13609047919584327 1101 16-17 0.44267011407182366 1101 18-19 0.4904063857514913 1101 20-21 0.5545897458479203 1101 22-23 0.4798410294469946 1101 24-25 0.47306512812554047 1101 26-27 0.5327673519501204 1101 28-29 0.534013799582624 1101 30-31 0.5116141745344365 1101 32-33 0.2723954982489616 1101 34-35 0.23518309239894108 1101 36-37 0.4105434824355072 1101 38-39 0.3085057377178373 1101 40-41 0.4750595789798737 1101 42-43 0.368844862163499 1101 44-45 0.31793934620636577 1101 46-47 0.2508848988012389 1101 48-49 0.2259152635507249 1101 50-51 0.05859946131847238 1101 52-53 0.1008620732632508 1101 54-55 0.10639298827205579 1101 56-57 -0.029022419327510818 1101 58-59 0.06362001775734427 1101 60-61 -0.002922361266875839 1101 62-63 -0.11873584869734799 1101 64-65 -0.07651856892872644 1101 66-67 -0.08609609293237241 1101 68-69 -0.03664934857826552 1101 70-71 -0.0028229593375712625 1101 72-73 -0.13051463726819534 1101 74-75 -0.13207084368659494 1101 76-77 -0.0881533446982985 1101 78-79 -0.19523640084905836 1101 80-81 -0.4343631116369835 1101 82-83 -0.48874102724067825 1101 84-85 -0.49259546679910926 1101 86-87 -0.4958283581918934 1101 88-89 -0.2808924549959393 1101 90-91 -0.4274869999196049 1101 92-93 -0.5589822395404482 1101 94-95 -0.5702966975199004 1101 96-97 -0.7624823561536367 1101 98-99 -0.8721592717321052 1101 100-101 -0.6478497885484238 1102 1 0.22285527440288888 1102 2 0.19741606588288718 1102 3 0.10206990192283527 1102 4 0.2716940552039375 1102 5 0.1610635037938124 1102 6 0.18948298848113154 1102 7 0.3056355891439395 1102 8 0.34111588494040035 1102 9 0.35869560379222065 1102 10-11 0.3609629347931289 1102 12-13 0.2643534726393071 1102 14-15 0.43550482619873776 1102 16-17 0.5983310275668643 1102 18-19 0.5086060960157752 1102 20-21 0.5134188087532721 1102 22-23 0.3439152460588417 1102 24-25 0.2708543229573621 1102 26-27 0.31567269531087305 1102 28-29 0.3060847120507333 1102 30-31 0.39783806865826676 1102 32-33 0.43898755414621604 1102 34-35 0.36041203446475834 1102 36-37 0.47336408856741485 1102 38-39 0.3769480742280251 1102 40-41 0.3565765139628283 1102 42-43 0.34496434773802775 1102 44-45 0.31402626891591723 1102 46-47 0.33454135160253173 1102 48-49 0.1881013125665092 1102 50-51 0.20953197961639347 1102 52-53 0.08936960529519666 1102 54-55 -0.03435565851457767 1102 56-57 -0.018361969449507853 1102 58-59 -0.07208916176015379 1102 60-61 0.016771392266328178 1102 62-63 0.031301091111210155 1102 64-65 -0.12279103815303216 1102 66-67 -0.19584496205577295 1102 68-69 -0.03272405772088405 1102 70-71 -0.23557939688436846 1102 72-73 -0.35747511416560585 1102 74-75 -0.6448262941777791 1102 76-77 -0.46969246350689176 1102 78-79 -0.4883051766907265 1102 80-81 -0.5437867010697026 1102 82-83 -0.6382131518928382 1102 84-85 -0.7485287677623376 1102 86-87 -0.9472166170158189 1102 88-89 -0.8472632210009472 1102 90-91 -0.7107059079170455 1102 92-93 -0.7407398067175599 1102 94-95 -0.7550455915689547 1102 96-97 -0.8154687975988075 1102 98-99 -1.0347455269328414 1102 100-101 -0.6921945082495533 1103 1 0.2611434489222617 1103 2 0.2391636725041657 1103 3 0.30909342203967327 1103 4 0.2565405263526941 1103 5 0.25543430057644656 1103 6 0.24784263700596654 1103 7 0.12321209364625219 1103 8 0.18403344070008387 1103 9 0.15320661203472952 1103 10-11 0.22092017986108203 1103 12-13 0.26276965693572407 1103 14-15 0.2805618450353293 1103 16-17 0.31330305148177473 1103 18-19 0.3376400689729664 1103 20-21 0.42497107565233705 1103 22-23 0.45351411573574296 1103 24-25 0.42458130441893616 1103 26-27 0.4377515619275485 1103 28-29 0.3150022884081096 1103 30-31 0.5641328324787054 1103 32-33 0.4750175836254371 1103 34-35 0.4146263966497443 1103 36-37 0.5477217714835305 1103 38-39 0.5525539775277224 1103 40-41 0.5682465310245775 1103 42-43 0.6651928056130458 1103 44-45 0.5710482442055493 1103 46-47 0.35470012060574874 1103 48-49 0.21612104515937602 1103 50-51 0.34464060549014164 1103 52-53 0.22940647517120993 1103 54-55 0.1423161755582285 1103 56-57 0.17436233578285254 1103 58-59 0.07662767533610548 1103 60-61 0.03866478153385344 1103 62-63 -0.10465645409460933 1103 64-65 -0.002684646148473746 1103 66-67 0.008393700506395874 1103 68-69 -0.12459860990490768 1103 70-71 -0.24328465169993763 1103 72-73 -0.033152618980320625 1103 74-75 -0.12549462075719475 1103 76-77 -0.17376144028690987 1103 78-79 -0.20766794282399204 1103 80-81 -0.16997971227142017 1103 82-83 -0.27592959321907884 1103 84-85 -0.275134860855097 1103 86-87 -0.29541429408612174 1103 88-89 -0.21481660213728304 1103 90-91 -0.25429161370445996 1103 92-93 -0.28483702195809 1103 94-95 -0.23660476850463397 1103 96-97 -0.13683650423660865 1103 98-99 -0.08749867874816175 1103 100-101 0.1503204115935297 1104 1 0.07524063751411347 1104 2 0.06473524975602984 1104 3 -0.005226168279016008 1104 4 0.11402193751572298 1104 5 0.14405580460904588 1104 6 0.11761824232858942 1104 7 0.16829032417575007 1104 8 0.1178992544737767 1104 9 0.13994338177962362 1104 10-11 0.24471884949439016 1104 12-13 0.17183503052729776 1104 14-15 0.2512825614192167 1104 16-17 0.2671542117737218 1104 18-19 0.12492412989370649 1104 20-21 0.2552773546018656 1104 22-23 0.14903917155280055 1104 24-25 0.020220545711339355 1104 26-27 0.08833437961301627 1104 28-29 0.1403685532433201 1104 30-31 0.22269322898920763 1104 32-33 0.2344429399255219 1104 34-35 0.26064497248921015 1104 36-37 0.3663676049171585 1104 38-39 0.3807305914566257 1104 40-41 0.33379395217546914 1104 42-43 0.268856801854362 1104 44-45 0.3617937328683567 1104 46-47 0.6027338871571004 1104 48-49 0.5719842671345887 1104 50-51 0.7419603277094211 1104 52-53 0.7154185137477995 1104 54-55 0.6853427260881872 1104 56-57 0.47762466102660284 1104 58-59 0.4304298302259042 1104 60-61 0.3926048543776446 1104 62-63 0.3903671869201233 1104 64-65 0.5280941642622352 1104 66-67 0.5167785344416274 1104 68-69 0.5076464151802753 1104 70-71 0.49901986344023186 1104 72-73 0.38650186886509275 1104 74-75 0.2440763942322981 1104 76-77 0.5328182329654751 1104 78-79 0.33964658039387885 1104 80-81 0.17652578856090173 1104 82-83 0.15126946402866892 1104 84-85 0.18417067771812512 1104 86-87 0.15868983613538745 1104 88-89 0.4017050541105185 1104 90-91 0.21229252683802713 1104 92-93 0.16599823612900622 1104 94-95 0.32167748143061914 1104 96-97 0.21951219856440218 1104 98-99 0.3776097637561797 1104 100-101 0.699695039118776 1105 1 -0.04320196573865687 1105 2 -0.010725880025798773 1105 3 -0.056590979335041425 1105 4 0.07969739236030904 1105 5 0.14591917931373644 1105 6 0.04878521770912414 1105 7 0.05154842005821081 1105 8 0.04445031057097282 1105 9 0.2147741437298052 1105 10-11 0.17185544521232998 1105 12-13 0.236653850611809 1105 14-15 0.27505907476415814 1105 16-17 0.2410919218193115 1105 18-19 0.11771787945466627 1105 20-21 0.23125960078176888 1105 22-23 0.08513355296046399 1105 24-25 0.19895385760106876 1105 26-27 0.23277133728409183 1105 28-29 0.2640472763879842 1105 30-31 0.23455375687144198 1105 32-33 0.18188856376784912 1105 34-35 0.2092385682478195 1105 36-37 0.303756458682912 1105 38-39 0.24571680100493865 1105 40-41 0.34246751776515083 1105 42-43 0.28521262878234666 1105 44-45 0.3075774778818001 1105 46-47 0.3393792396270001 1105 48-49 0.3728744537308657 1105 50-51 0.5059531995617377 1105 52-53 0.5038191910697165 1105 54-55 0.42900577322343025 1105 56-57 0.3315905250372211 1105 58-59 0.24873878070967947 1105 60-61 0.29655555998292726 1105 62-63 0.22159174575507024 1105 64-65 0.04216467672726054 1105 66-67 0.26176378272521816 1105 68-69 0.2509323507681458 1105 70-71 0.1573276862865285 1105 72-73 0.32215952182633956 1105 74-75 0.2662035067039703 1105 76-77 0.15782146799100616 1105 78-79 0.011462945556168336 1105 80-81 0.13019929869300384 1105 82-83 0.09475171945149796 1105 84-85 0.018145008252698602 1105 86-87 -0.09119740011760769 1105 88-89 0.23154178699353878 1105 90-91 0.2024548142044189 1105 92-93 0.010521984913559379 1105 94-95 0.166830673753811 1105 96-97 -0.07624769968942502 1105 98-99 -0.020832155519762807 1105 100-101 0.6334948631333894 1106 1 0.05629094547560598 1106 2 0.15074051227056273 1106 3 0.23419102920447088 1106 4 0.17414157094205507 1106 5 0.1873698325383799 1106 6 0.2085505318436205 1106 7 0.24993871004351575 1106 8 0.24642310041005544 1106 9 0.1803856899695333 1106 10-11 0.20865460898551902 1106 12-13 0.29949182856648093 1106 14-15 0.33888428953690664 1106 16-17 0.3932836939005355 1106 18-19 0.4515366337638582 1106 20-21 0.5138867141686632 1106 22-23 0.4449871335106579 1106 24-25 0.2732979300149765 1106 26-27 0.16605863206024907 1106 28-29 0.17334024112926727 1106 30-31 0.0863875891404362 1106 32-33 0.17179242781629966 1106 34-35 0.2535698438038665 1106 36-37 0.5120454815666022 1106 38-39 0.5187513667004993 1106 40-41 0.5450455883463761 1106 42-43 0.6196021016829576 1106 44-45 0.7104228290952861 1106 46-47 0.6587625356509292 1106 48-49 0.501753833597256 1106 50-51 0.49618983508702286 1106 52-53 0.35683788476221423 1106 54-55 0.31135576905825246 1106 56-57 0.4373092759788193 1106 58-59 0.521529417379945 1106 60-61 0.4800847898141356 1106 62-63 0.5223429408078957 1106 64-65 0.5949933672116785 1106 66-67 0.6406626591894522 1106 68-69 0.7409474340452036 1106 70-71 0.6133905521800713 1106 72-73 0.56615744986971 1106 74-75 0.4202082408324088 1106 76-77 0.5210001046825354 1106 78-79 0.6408146748157755 1106 80-81 0.7635560279437463 1106 82-83 0.6369809072763992 1106 84-85 0.8093247030777704 1106 86-87 0.9403108744157649 1106 88-89 1.084691196512484 1106 90-91 1.0498471885532794 1106 92-93 1.1067159831517763 1106 94-95 1.2955888645404272 1106 96-97 1.4260444798706935 1106 98-99 1.5827884956311564 1106 100-101 1.8767178014124788 1107 1 0.03300530235812005 1107 2 0.028814394895022133 1107 3 0.1700880946846155 1107 4 0.09644731889572 1107 5 0.16018763525130453 1107 6 0.02265631184175021 1107 7 -0.025866932408568744 1107 8 0.049258159854282724 1107 9 0.037038812159622125 1107 10-11 0.06819282726084452 1107 12-13 0.08889207178921055 1107 14-15 0.09523408374315778 1107 16-17 0.019824739267967573 1107 18-19 0.2424729974877735 1107 20-21 0.09847643560071617 1107 22-23 0.11114818057662035 1107 24-25 -6.167826836502854E-4 1107 26-27 0.09196828768732956 1107 28-29 0.01904286851257808 1107 30-31 -0.14121762132427307 1107 32-33 -0.12385473462899199 1107 34-35 -0.0373820606090689 1107 36-37 -0.033605183005796846 1107 38-39 -0.07541732901106002 1107 40-41 -0.08491681631915782 1107 42-43 0.009349011712444621 1107 44-45 0.16208493861905282 1107 46-47 0.13547010716217756 1107 48-49 0.07915894127453527 1107 50-51 0.2365585477839005 1107 52-53 0.1093959697290714 1107 54-55 0.1882949625008692 1107 56-57 0.050763966771633307 1107 58-59 0.18304511172479465 1107 60-61 0.18875443679096549 1107 62-63 0.005283423033283441 1107 64-65 -0.004134340479787113 1107 66-67 -0.09327833697309273 1107 68-69 0.057614363371406796 1107 70-71 0.013112614617746488 1107 72-73 0.11882178061760129 1107 74-75 0.19906095274830804 1107 76-77 -0.09642315276845537 1107 78-79 -0.24276126837272116 1107 80-81 -0.32170588300941816 1107 82-83 -0.3446318310105134 1107 84-85 -0.23848721168396025 1107 86-87 -0.3290839054886803 1107 88-89 -0.22624056191428465 1107 90-91 -0.21612601317011837 1107 92-93 -0.15056953564019793 1107 94-95 -0.061154437422601404 1107 96-97 -2.444998983897051E-4 1107 98-99 0.11711393779658863 1107 100-101 0.34387704225638416 1108 1 0.22775806669631038 1108 2 0.2135729950316687 1108 3 0.15656322450477944 1108 4 0.17864247987846937 1108 5 0.24879642497953114 1108 6 0.13603131028457938 1108 7 0.16954032295504362 1108 8 0.1311658430806233 1108 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-1.4700132238765349 2316 92-93 -1.1511725239623622 2316 94-95 -0.9590715622137935 2316 96-97 -1.2977611756310985 2316 98-99 -1.430832506964201 2316 100-101 -1.473035950733827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8471.0 3 1719.0 4 1447.0 5 1649.0 6 1891.0 7 2258.0 8 2706.0 9 3107.0 10 4039.0 11 5353.0 12 6803.0 13 8801.0 14 9365.0 15 10145.0 16 11857.0 17 14532.0 18 13229.0 19 9802.0 20 9801.0 21 10460.0 22 11481.0 23 13097.0 24 14764.0 25 16842.0 26 19683.0 27 23238.0 28 27026.0 29 31916.0 30 38586.0 31 46006.0 32 56542.0 33 70654.0 34 92400.0 35 127305.0 36 185949.0 37 273551.0 38 254767.0 39 42919.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61495992346815 19.788226741829305 14.383975332529822 23.212838002172724 2 15.578125147630686 21.365518060547817 39.66942873841394 23.38692805340755 3 18.093410169235213 25.404995233761714 31.45285204678205 25.048742550221032 4 12.831655344346556 17.667138636219455 38.07822456662262 31.422981452811367 5 13.324418149153733 37.075211735624265 35.07767016520106 14.522699950020938 6 30.145907691933793 36.98432233225476 18.3613620391567 14.508407936654743 7 26.380321690648817 33.607251040952576 22.531785778207027 17.480641490191577 8 24.967004524128914 34.694219408769705 21.474887639766404 18.86388842733498 9 25.973052377013005 16.758322953118316 21.728815251965944 35.53980941790273 10-11 23.791711001766203 26.676474400868518 28.95559108364886 20.576223513716425 12-13 24.869392132840396 24.83242525347632 28.69895827671549 21.59922433696779 14-15 22.322396368353488 26.210616329720676 27.137358417215495 24.329628884710342 16-17 21.300744231270148 28.97235002957803 27.907507076937005 21.819398662214816 18-19 21.140867418245005 27.56642661947438 30.37591410571869 20.916791856561925 20-21 21.970837734137106 27.021525879764134 30.215184536714773 20.79245184938399 22-23 21.78396808232566 26.874410273417 29.982830809174104 21.358790835083237 24-25 21.51859453202506 27.098743796138532 30.187454709869655 21.195206961966758 26-27 21.41345639641601 27.221622396363276 30.022756574290483 21.342164632930235 28-29 21.40653746382307 27.26608513221055 30.045304805431982 21.282072598534405 30-31 21.653495021736507 27.077942283970323 29.884480771628308 21.38408192266486 32-33 21.360368932373085 27.30637581593345 29.753290145410737 21.579965106282724 34-35 21.810812702283012 27.230078852552186 29.60336385264854 21.35574459251626 36-37 21.859339654295333 27.037874360056712 29.496751577534607 21.606034408113345 38-39 21.553037168898133 27.266209822068678 29.59447376750704 21.586279241526146 40-41 21.836029907396327 27.0735427446033 29.624758045269452 21.46566930273092 42-43 21.643289283826643 27.143182257017866 29.396832074091634 21.81669638506386 44-45 21.665277272842566 27.274854790026804 29.285355563634518 21.774512373496115 46-47 21.72478028567428 27.320537041133065 28.902960436202186 22.05172223699047 48-49 21.70529603066625 27.46377630306872 28.757525673039908 22.07340199322512 50-51 21.645248754906298 27.63901685967141 28.597782982298764 22.117951403123524 52-53 21.75877819630255 27.564760845564358 28.56319949560549 22.1132614625276 54-55 21.679590391324023 27.512854387741502 28.52097932136409 22.286575899570384 56-57 21.54277065288624 27.750184462664144 28.440524570562125 22.266520313887487 58-59 21.58433949794034 27.426804799840433 28.59890838957882 22.389947312640402 60-61 21.650184467925694 27.262997494804925 28.738065008004256 22.348753029265126 62-63 21.493261944120537 27.256897004196233 28.840123695609236 22.40971735607399 64-65 21.39460427529434 27.218304963418554 28.774289100127298 22.6128016611598 66-67 21.358807793634004 27.30952091770849 28.573308654146413 22.758362634511098 68-69 21.398089970399177 27.153012473351986 28.653692016777292 22.795205539471546 70-71 21.42224946915841 27.016724385962775 28.599369892045296 22.961656252833524 72-73 21.48300936222268 26.969453185841846 28.544054951382343 23.003482500553126 74-75 21.36919052385629 27.2351685347267 28.358406371644772 23.037234569772234 76-77 21.45839350980287 26.980716245989456 28.48789582024108 23.072994423966595 78-79 21.403759115017017 27.01737538231082 28.489067051696708 23.08979845097545 80-81 21.439970402765205 26.948025267218867 28.440737725957067 23.171266604058864 82-83 21.46809420219271 26.85659436994708 28.509786573157136 23.165524854703072 84-85 21.500831164526605 26.66456973072975 28.4397274659713 23.394871638772347 86-87 21.503999902598128 26.558582153406597 28.550653370409172 23.3867645735861 88-89 21.620658130027543 26.433061883136276 28.49367970996307 23.452600276873113 90-91 21.611457042855317 26.398249494408898 28.613783878325243 23.37650958441054 92-93 21.776529985358472 26.20390016698376 28.520698408918072 23.49887143873969 94-95 21.71136545872227 26.032560704534614 28.56196988202496 23.694103954718155 96-97 21.80247163359438 25.81553521893482 28.57390321132376 23.80808993614704 98-99 21.830910627494685 25.719825454210827 28.4760804740262 23.973183444268294 100-101 22.156444670530114 25.28625805740246 28.793058779042667 23.764238493024752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1828.0 1 1246.0 2 709.0 3 756.0 4 691.5 5 702.0 6 915.0 7 1444.0 8 2115.5 9 2353.0 10 2089.5 11 1845.0 12 1866.0 13 1949.0 14 2022.5 15 2186.5 16 2416.0 17 2556.0 18 2677.0 19 2915.5 20 3282.5 21 3842.0 22 4431.0 23 5108.0 24 5983.0 25 7413.5 26 9592.5 27 12238.0 28 14503.0 29 16653.5 30 19389.0 31 22506.0 32 25928.5 33 28575.5 34 31353.5 35 34791.5 36 38547.5 37 41777.5 38 43123.5 39 43837.5 40 44904.0 41 46524.5 42 48207.0 43 50190.0 44 52515.5 45 54141.0 46 54789.0 47 55150.0 48 56284.0 49 56066.5 50 53851.5 51 51852.0 52 49957.5 53 48127.5 54 45947.0 55 42177.5 56 37878.5 57 33575.5 58 29965.5 59 26492.0 60 22073.0 61 17818.0 62 14238.5 63 10965.5 64 8049.5 65 6023.5 66 4566.5 67 3615.0 68 2749.0 69 1954.0 70 1461.5 71 1093.0 72 834.0 73 688.0 74 572.0 75 429.5 76 317.5 77 215.0 78 146.0 79 113.0 80 86.5 81 72.0 82 57.0 83 39.5 84 29.5 85 22.0 86 19.5 87 16.5 88 13.5 89 9.0 90 7.5 91 5.5 92 6.0 93 5.5 94 1.5 95 2.0 96 2.5 97 2.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2692424826366897 2 0.16345902474389623 3 0.19620473209160175 4 0.16372853673852755 5 0.23878762724335048 6 0.19438552612784032 7 0.2391245172366396 8 0.24646871909034312 9 0.14674928107675433 10-11 0.2034815559466474 12-13 0.2094445088278654 14-15 0.16992731261504793 16-17 0.2816737233890594 18-19 0.22999479841850362 20-21 0.19542988510703665 22-23 0.2428976851614781 24-25 0.2313086693923313 26-27 0.3389787112475441 28-29 0.31196013378575416 30-31 0.28841152325484243 32-33 0.1892311092305163 34-35 0.3547114739341474 36-37 0.3980692160704612 38-39 0.37839484046237476 40-41 0.3412695632019103 42-43 0.37479011753418084 44-45 0.38415565934761925 46-47 0.38856891825970713 48-49 0.4081085378704779 50-51 0.40170762799798404 52-53 0.40130336000603706 54-55 0.3958457421147529 56-57 0.3736110025576688 58-59 0.3474346500791018 60-61 0.3125328467743457 62-63 0.3837850803550012 64-65 0.41508216073156334 66-67 0.4221568505906355 68-69 0.426738554499368 70-71 0.42596370751480295 72-73 0.4179457256745211 74-75 0.41205015079196106 76-77 0.39571098611743716 78-79 0.3700062796294749 80-81 0.38051724742009646 82-83 0.39160092819930953 84-85 0.3919041291932697 86-87 0.38762562627849756 88-89 0.3954751631221347 90-91 0.4171371896906272 92-93 0.41612651971075976 94-95 0.4250877935322511 96-97 0.42552575052352704 98-99 0.42252742958325357 100-101 0.3951719621281745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1484164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.88253374044004 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8901020114256 55.439000445276896 2 10.562022887402627 14.128297042680124 3 2.8776629515821854 5.773961683584295 4 1.2041406395671428 3.2214390781633795 5 0.6407892156892009 2.1428803169421546 6 0.381605121829476 1.5313630461770797 7 0.2626774493702677 1.2297973359251764 8 0.18960442165843625 1.0144979303125585 9 0.14106296230836146 0.8491183502503866 >10 0.788475773943351 9.539176898026607 >50 0.044495176882218594 2.062570461479054 >100 0.0164529942677122 1.787842376093653 >500 5.046633741390755E-4 0.24390037136249043 >1k 3.0279802448344534E-4 0.6187630235008545 >5k 1.009326748278151E-4 0.41739164022537456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6183 0.4165981657013646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4812 0.32422292954147924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3094 0.20846752784732686 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.737799865783026E-5 0.0 12-13 0.0 0.0 0.0 6.737799865783026E-5 0.0 14-15 0.0 0.0 0.0 6.737799865783026E-5 0.0 16-17 0.0 0.0 0.0 1.0106699798674539E-4 0.0 18-19 0.0 0.0 0.0 2.3582299530240593E-4 0.0 20-21 0.0 0.0 0.0 5.053349899337271E-4 3.368899932891513E-5 22-23 0.0 0.0 0.0 0.0010443589791963692 6.737799865783026E-5 24-25 0.0 0.0 0.0 0.0024256079516818893 6.737799865783026E-5 26-27 0.0 0.0 0.0 0.0045817039087324585 6.737799865783026E-5 28-29 0.0 0.0 0.0 0.013879867723513035 6.737799865783026E-5 30-31 0.0 6.737799865783026E-5 0.0 0.04224600515845958 6.737799865783026E-5 32-33 0.0 6.737799865783026E-5 0.0 0.09752965305720931 6.737799865783026E-5 34-35 0.0 6.737799865783026E-5 0.0 0.18417775933117903 6.737799865783026E-5 36-37 0.0 6.737799865783026E-5 0.0 0.31425098574012034 6.737799865783026E-5 38-39 0.0 6.737799865783026E-5 0.0 0.5055034349303715 6.737799865783026E-5 40-41 3.368899932891513E-5 6.737799865783026E-5 0.0 0.7195970256656272 6.737799865783026E-5 42-43 6.737799865783026E-5 6.737799865783026E-5 0.0 0.9267506825391264 6.737799865783026E-5 44-45 6.737799865783026E-5 6.737799865783026E-5 0.0 1.181338450467738 6.737799865783026E-5 46-47 6.737799865783026E-5 6.737799865783026E-5 0.0 1.4859543823997887 6.737799865783026E-5 48-49 6.737799865783026E-5 6.737799865783026E-5 0.0 1.805393474036562 6.737799865783026E-5 50-51 6.737799865783026E-5 6.737799865783026E-5 0.0 2.1402621273659785 1.3475599731566052E-4 52-53 6.737799865783026E-5 6.737799865783026E-5 0.0 2.4789039486202333 1.3475599731566052E-4 54-55 6.737799865783026E-5 6.737799865783026E-5 0.0 2.842273495381912 1.3475599731566052E-4 56-57 6.737799865783026E-5 6.737799865783026E-5 0.0 3.261802604024892 1.3475599731566052E-4 58-59 6.737799865783026E-5 6.737799865783026E-5 0.0 3.727721464743788 1.3475599731566052E-4 60-61 6.737799865783026E-5 6.737799865783026E-5 0.0 4.227363013790929 1.3475599731566052E-4 62-63 6.737799865783026E-5 6.737799865783026E-5 0.0 4.727577275826661 1.3475599731566052E-4 64-65 6.737799865783026E-5 6.737799865783026E-5 0.0 5.250329478413438 1.3475599731566052E-4 66-67 6.737799865783026E-5 6.737799865783026E-5 0.0 5.832239563821788 1.3475599731566052E-4 68-69 6.737799865783026E-5 6.737799865783026E-5 0.0 6.475632073005409 1.3475599731566052E-4 70-71 6.737799865783026E-5 1.0106699798674539E-4 0.0 7.179934292975709 1.3475599731566052E-4 72-73 6.737799865783026E-5 1.3475599731566052E-4 0.0 7.8989249166534155 1.3475599731566052E-4 74-75 6.737799865783026E-5 1.3475599731566052E-4 0.0 8.633345102023766 1.3475599731566052E-4 76-77 6.737799865783026E-5 1.3475599731566052E-4 0.0 9.408124708590154 1.3475599731566052E-4 78-79 6.737799865783026E-5 1.3475599731566052E-4 0.0 10.25142773979156 1.3475599731566052E-4 80-81 6.737799865783026E-5 1.3475599731566052E-4 0.0 11.145533781980967 1.3475599731566052E-4 82-83 6.737799865783026E-5 1.3475599731566052E-4 0.0 12.065041329664378 1.3475599731566052E-4 84-85 6.737799865783026E-5 1.3475599731566052E-4 0.0 12.997721276085393 1.3475599731566052E-4 86-87 6.737799865783026E-5 1.3475599731566052E-4 0.0 13.956779708980948 1.3475599731566052E-4 88-89 6.737799865783026E-5 1.3475599731566052E-4 0.0 14.96074557798195 1.3475599731566052E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3310 0.0 50.825455 1 GTATCAA 5950 0.0 39.536133 1 ATCAACG 7320 0.0 31.563824 3 TATCAAC 7410 0.0 31.372532 2 TCAACGC 7425 0.0 31.117464 4 CAACGCA 7610 0.0 30.298656 5 AACGCAG 7690 0.0 29.98244 6 ACGCAGA 8545 0.0 26.705748 7 CGCAGAG 8555 0.0 26.397247 8 GCAGAGT 9020 0.0 23.563684 9 GAGTACT 5445 0.0 23.40818 12-13 GTACATG 4575 0.0 22.437199 1 TACATGG 4735 0.0 21.341814 2 CAGAGTA 8800 0.0 20.847021 10-11 GTACTTT 6245 0.0 20.827606 14-15 AGAGTAC 7910 0.0 20.73236 10-11 ACATGGG 4750 0.0 20.57526 3 AGTACTT 5815 0.0 19.796263 12-13 GTGTACG 170 7.9259815E-5 19.56839 1 CATGGGG 2435 0.0 19.094084 4 >>END_MODULE