FastQCFastQC Report
Thu 26 May 2016
SRR938814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484164
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58200.3921399521885721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.3440994391455392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.21419465773324242No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT19350.13037642740290156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.049.2836271
GTATCAA58450.037.021211
CGTAATA953.5131445E-529.9728762
ATCAACG73150.029.2592373
TCAACGC74750.028.6329544
TATCAAC74950.028.5565472
CAACGCA76900.027.8941365
AACGCAG78750.027.3556826
TAGGACG1806.7666406E-926.365034
ACGCAGA88650.024.0322957
CGCAGAG89200.023.7777258
GAGTACT58150.021.66491912-13
GCAGAGT97000.021.4254519
GTACATG46050.021.2745931
TACATGG46700.020.6290842
AGAGTAC82750.020.04109610-11
TCCGCAC1908.318675E-619.9819163
ACATGGG46350.019.9657523
CAGAGTA92600.019.7796510-11
GTACTTT64300.019.66656514-15
Produced by FastQC (version 0.11.5)