FastQCFastQC Report
Thu 26 May 2016
SRR938813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147225
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.7831550348106641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.7566649685854984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.46187807777211753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.391237901171676No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.3226354219731703No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.19493971811852606No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2330.15826116488368144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.13924265579894718No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.10799796230259806No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1560.10596026490066225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.031.8410931
TTGGAGT600.00401325431.5474644
TCTACAG1102.7930273E-630.1134913
GGTCTAC953.5443518E-529.9074251
GAACAAC650.005955882429.1009356
GTTTAGA700.00849527827.05911
GTCCTGG1257.5118132E-626.5179181
GTATCAA15700.024.1291311
GAGTACT9200.022.35948212-13
CCGCCCG550.002844301821.62737584-85
TATCAAC17450.021.1521683
GTCTACA1605.097333E-520.7030242
ATCAACG17900.020.6204094
TCAACGC18050.020.4560095
GTACATG8350.020.4158481
GTACTTT10050.020.23311214-15
CAACGCA18400.020.046436
TCGCCGT600.00470896519.82509492-93
AACGCAG18700.019.724837
CATGGGA4650.019.3355434
Produced by FastQC (version 0.11.5)