FastQCFastQC Report
Thu 26 May 2016
SRR938813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147225
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.7817965698760402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.7722873153336729No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT10470.7111563932755985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.45576498556631007No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT5550.3769740193581253RNA PCR Primer, Index 31 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990.33893700118865683No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.25810833757853624No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT2790.18950585838003056RNA PCR Primer, Index 31 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.18610969604347088No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2340.15894039735099338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.15690269994905756No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1610.10935642723722194No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT500.001620842337.9540754
CGCAGAA500.001620842337.9540759
GGTATCA11850.034.9738271
GATTTGC600.00396325831.6283976
TAGGCAG600.00396325831.6283975
TTAGATT650.005862286329.1954423
CAGTACA856.425744E-427.907414
GTATCAA14900.027.7013822
TTAGGCA700.00841774327.1100544
TTAATGC950.001230120324.9697883
TATCAAC17650.023.9228953
ATCAACG17850.023.9206354
TCAACGC17850.023.9206355
AACGCAG17950.023.7873747
TAAGGCT1000.001658460823.7212965
CAACGCA18350.023.2688476
CTCCGAC652.9550126E-421.90030394-95
GAGTACT8950.021.7334812-13
ACGCAGA20150.020.9547948
ATGGGGA1404.8636523E-420.332545
Produced by FastQC (version 0.11.5)