FastQCFastQC Report
Thu 26 May 2016
SRR938812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147245
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.8007063058168359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.7844069408129308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.4760772861557268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.4108798261401066No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.3164793371591565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.19491323983836462No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2420.16435193045604266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.13718632211620088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.11681211586131958No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1570.10662501273387892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGAC550.00258548734.509553
GGTATCA10900.033.1638871
TCGGAAG300.00393996331.67689568-69
ATTAGTG600.00395994331.6337539
CATGGGT902.4158744E-531.633754
AAGATAT404.495014E-429.67685118-19
TACAGTA804.5021228E-429.6566417
CCCTAAT650.005789445729.270151
GAGTACC700.00831333327.1794261
GTGTGCC700.00841072727.1146479
GTATCAA14400.025.3729062
CAAGACT950.00122889324.9740144
GGTGTGC1201.712972E-423.7253158
GTACATG7100.022.7771241
GAGTACT10100.022.09603112-13
ACAGTAA1100.00288290421.5684688
ACATGGG7100.021.38623
TACATGG7600.021.2279132
GGGAAGC1353.800831E-421.0891697
TATCAAC17450.020.93813
Produced by FastQC (version 0.11.5)