##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938812_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147245 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.298937145573703 33.0 31.0 34.0 30.0 34.0 2 31.34434445991375 33.0 31.0 34.0 28.0 34.0 3 31.307990084552955 33.0 31.0 34.0 28.0 34.0 4 34.86266426703793 37.0 35.0 37.0 32.0 37.0 5 34.882535909538525 37.0 35.0 37.0 32.0 37.0 6 34.963489422391255 37.0 35.0 37.0 32.0 37.0 7 34.88029474685049 37.0 35.0 37.0 32.0 37.0 8 34.865272165438554 37.0 35.0 37.0 32.0 37.0 9 36.41026180855037 39.0 37.0 39.0 32.0 39.0 10-11 36.305986620937894 39.0 37.0 39.0 32.0 39.0 12-13 36.18461407857652 39.0 37.0 39.0 32.0 39.0 14-15 37.28829841420762 40.0 37.0 41.0 32.0 41.0 16-17 37.16749974532242 40.0 37.0 41.0 32.0 41.0 18-19 37.14786919759584 40.0 37.0 41.0 31.5 41.0 20-21 37.08034228666508 40.0 37.0 41.0 31.0 41.0 22-23 37.023359027471216 40.0 37.0 41.0 31.0 41.0 24-25 36.91246222282591 40.0 37.0 41.0 31.0 41.0 26-27 36.740670311385784 40.0 36.0 41.0 30.0 41.0 28-29 36.547302115521745 39.5 36.0 41.0 30.0 41.0 30-31 36.230612244897955 39.0 35.5 41.0 30.0 41.0 32-33 35.93983157322829 39.0 35.0 41.0 29.5 41.0 34-35 35.582332167475975 39.0 35.0 41.0 27.5 41.0 36-37 35.299402356616525 38.5 35.0 41.0 26.0 41.0 38-39 35.08989778939862 38.0 35.0 40.5 25.0 41.0 40-41 34.863289076029744 38.5 35.0 40.5 24.5 41.0 42-43 34.71171177289551 39.0 35.0 41.0 23.5 41.0 44-45 34.38562260178614 39.0 35.0 41.0 20.0 41.0 46-47 33.8694488777208 38.0 34.0 41.0 12.0 41.0 48-49 33.39144283337295 38.0 33.0 41.0 4.0 41.0 50-51 32.16992767156779 37.0 31.5 39.5 2.0 40.5 52-53 32.21888009779619 37.5 32.5 39.5 2.0 40.5 54-55 32.66705490848585 38.0 33.0 40.0 2.0 41.0 56-57 32.52227919453971 38.0 33.0 40.0 2.0 41.0 58-59 32.29977927943224 38.0 32.5 40.0 2.0 41.0 60-61 31.97344222214676 37.0 32.0 40.0 2.0 41.0 62-63 31.58080410200686 36.5 31.0 40.0 2.0 41.0 64-65 31.171038065808688 36.0 31.0 39.5 2.0 41.0 66-67 30.788359536826377 35.5 30.5 39.0 2.0 41.0 68-69 30.342544738361234 35.0 30.0 39.0 2.0 40.5 70-71 29.87408400964379 35.0 29.0 38.0 2.0 40.0 72-73 29.417409759244798 35.0 29.0 37.0 2.0 40.0 74-75 28.92374274168902 35.0 28.5 37.0 2.0 39.0 76-77 28.41151821793609 34.5 27.5 36.0 2.0 39.0 78-79 27.913358687901116 34.0 26.5 36.0 2.0 38.5 80-81 27.408397568678055 34.0 26.0 35.0 2.0 37.0 82-83 26.955974735984242 34.0 25.0 35.0 2.0 37.0 84-85 26.554402526401574 34.0 24.0 35.0 2.0 36.0 86-87 26.068803694522735 34.0 21.5 35.0 2.0 36.0 88-89 25.55771333491799 33.5 18.5 35.0 2.0 35.5 90-91 25.086722808923902 33.0 10.5 35.0 2.0 35.0 92-93 24.595344493870762 33.0 2.0 35.0 2.0 35.0 94-95 24.033861930795613 32.5 2.0 35.0 2.0 35.0 96-97 23.40736527556114 32.0 2.0 35.0 2.0 35.0 98-99 22.673876871880196 31.5 2.0 35.0 2.0 35.0 100-101 20.55544840232266 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.14558729703233553 1101 2 0.1297350599783087 1101 3 0.11161719233003353 1101 4 -0.0965741225537613 1101 5 -0.3518706565661276 1101 6 -0.14297602451286195 1101 7 -0.24828619607375657 1101 8 -0.24157541330232135 1101 9 -0.3431589322577224 1101 10-11 -0.39103018301339887 1101 12-13 9.519008812119978E-4 1101 14-15 -0.008086691699482174 1101 16-17 -0.2318033432805109 1101 18-19 0.27087658772942547 1101 20-21 0.035694676519717916 1101 22-23 -0.15569925107697458 1101 24-25 0.1849818910928107 1101 26-27 0.49249079288427566 1101 28-29 0.5031598178429135 1101 30-31 0.5751009826789684 1101 32-33 0.4882788364614967 1101 34-35 0.056413485465334645 1101 36-37 -0.0063377406630777955 1101 38-39 0.33116201722129546 1101 40-41 0.2753216842875972 1101 42-43 0.8291964345332659 1101 44-45 0.5231322276686683 1101 46-47 0.998889777520013 1101 48-49 0.9855750030621095 1101 50-51 1.0560860483785817 1101 52-53 0.5891202901013486 1101 54-55 0.38869592040381207 1101 56-57 -0.06567779377249394 1101 58-59 0.48459779090674004 1101 60-61 0.6960919975109192 1101 62-63 0.9833645686015586 1101 64-65 0.42005461801850785 1101 66-67 0.5456232883335232 1101 68-69 0.49765312937749684 1101 70-71 0.4560238634912288 1101 72-73 0.6813774087586744 1101 74-75 0.7275682767201808 1101 76-77 -0.010771271014402117 1101 78-79 0.08440829704704456 1101 80-81 -0.15042611980410925 1101 82-83 0.41263141787864654 1101 84-85 0.12492820866969723 1101 86-87 0.17167874274343475 1101 88-89 0.09593233405248469 1101 90-91 -0.3284813268989559 1101 92-93 -0.619026955743962 1101 94-95 -1.3530399064668863 1101 96-97 -0.6083580672250406 1101 98-99 -1.7866600614487993 1101 100-101 -2.629144928700594 1102 1 0.5502170994550646 1102 2 0.038992937496530544 1102 3 0.3519317191049858 1102 4 0.49207306794156125 1102 5 0.6915776588789768 1102 6 0.49807316334226925 1102 7 0.6209670898127158 1102 8 0.6427154665691148 1102 9 0.8727051554424463 1102 10-11 0.5938174322078567 1102 12-13 0.8163683044856498 1102 14-15 0.6216843006669279 1102 16-17 1.09856456121355 1102 18-19 0.8530667960747138 1102 20-21 1.280313858061355 1102 22-23 1.4163032743204838 1102 24-25 1.1109131314073366 1102 26-27 1.1383898917765407 1102 28-29 1.4859411987761604 1102 30-31 1.3770581657128886 1102 32-33 1.1493590178309532 1102 34-35 1.5269983445596367 1102 36-37 1.2698202114691597 1102 38-39 1.6245672861187401 1102 40-41 1.9075032833233436 1102 42-43 1.6246392400202652 1102 44-45 1.7340029158394756 1102 46-47 1.6655660100861596 1102 48-49 0.817435187875283 1102 50-51 1.1532277576135002 1102 52-53 0.7560834996519361 1102 54-55 0.34869132877205544 1102 56-57 0.6589677896517188 1102 58-59 0.24560335826024016 1102 60-61 0.6242329606056813 1102 62-63 0.08211334894937394 1102 64-65 0.13580391492489596 1102 66-67 0.3212072864968718 1102 68-69 0.3255817295037673 1102 70-71 0.551156733835029 1102 72-73 0.37347980214672916 1102 74-75 0.12239121192078173 1102 76-77 0.809753322913167 1102 78-79 1.0323506720685671 1102 80-81 1.038433433659705 1102 82-83 0.881379624272494 1102 84-85 1.1223454158096828 1102 86-87 0.7959971724053752 1102 88-89 0.49942197418334544 1102 90-91 0.8523428710003742 1102 92-93 0.8892953768049665 1102 94-95 1.1291675204974716 1102 96-97 0.2862926817848894 1102 98-99 0.36779095157995556 1102 100-101 0.7160596858893165 1103 1 0.5077580105563477 1103 2 0.48508979729308166 1103 3 0.7763567522761541 1103 4 0.6661111042086958 1103 5 0.4124423205331098 1103 6 0.6559126149123742 1103 7 0.3658808447883928 1103 8 0.46082692167747297 1103 9 0.4015087821562915 1103 10-11 0.7998656409650451 1103 12-13 0.4136427405758738 1103 14-15 0.5040372418433918 1103 16-17 0.4508975996921052 1103 18-19 0.5821136779853688 1103 20-21 0.6153702490387971 1103 22-23 -0.1395901356750926 1103 24-25 0.09396819553824542 1103 26-27 0.01463381039662437 1103 28-29 0.23679812049221027 1103 30-31 0.16995953678763698 1103 32-33 0.05305207400078871 1103 34-35 0.0966301446480955 1103 36-37 0.08610725260140129 1103 38-39 -0.43679936580519296 1103 40-41 -0.13313139159924958 1103 42-43 0.4724665288305303 1103 44-45 0.1305918145568583 1103 46-47 0.020359243168869057 1103 48-49 0.47707141078549853 1103 50-51 0.39836265367718937 1103 52-53 0.11496784286291017 1103 54-55 0.23941998865706893 1103 56-57 -0.22968767253315292 1103 58-59 -0.029855509497394905 1103 60-61 -0.19282807433461358 1103 62-63 -0.6042533029745556 1103 64-65 -0.1848230200596248 1103 66-67 -0.6942173043921152 1103 68-69 -0.6068596681079086 1103 70-71 -0.6330814351167611 1103 72-73 -0.7423428427006833 1103 74-75 -1.2225888853812954 1103 76-77 -0.9532387160076645 1103 78-79 -1.0428649404922439 1103 80-81 -0.7493540497547144 1103 82-83 -0.5001716804599248 1103 84-85 -1.4769745753446735 1103 86-87 -2.0096750965021855 1103 88-89 -2.1933301266569885 1103 90-91 -1.9102898136524367 1103 92-93 -2.3413271353577905 1103 94-95 -2.33771660157241 1103 96-97 -2.566289140417677 1103 98-99 -2.3722853420954095 1103 100-101 -1.8825968820009997 1104 1 -0.41684845174688334 1104 2 -0.3341935498485924 1104 3 -0.2807062137553338 1104 4 -0.2754796165866651 1104 5 0.11490710926550207 1104 6 0.4727104608112924 1104 7 0.634522104109017 1104 8 0.6623596474438358 1104 9 0.2582817730427891 1104 10-11 0.13654749680597433 1104 12-13 0.20501598001249022 1104 14-15 -0.029724150037296226 1104 16-17 0.1355496295181169 1104 18-19 -0.02755906435100286 1104 20-21 0.40498292509513334 1104 22-23 0.07260953292473715 1104 24-25 0.3192685269384157 1104 26-27 -0.1714163138784386 1104 28-29 0.03417798793215354 1104 30-31 0.1834226685191993 1104 32-33 -0.22946656725853387 1104 34-35 0.005236192701111975 1104 36-37 0.1961942451448948 1104 38-39 -0.11513407997254888 1104 40-41 -0.2937569094120107 1104 42-43 -0.39709643720095045 1104 44-45 0.4403991882105487 1104 46-47 0.699056426267461 1104 48-49 0.6563899691947768 1104 50-51 1.0969956279936781 1104 52-53 1.1710469646524686 1104 54-55 0.6579680416810945 1104 56-57 0.49649294055714677 1104 58-59 0.506960977660853 1104 60-61 0.8716913954251169 1104 62-63 1.1663556448299204 1104 64-65 0.9540025806031736 1104 66-67 0.6008324056327403 1104 68-69 0.38941208002796657 1104 70-71 0.7225005864242497 1104 72-73 0.5301302310358516 1104 74-75 0.07484276333452655 1104 76-77 -0.0022238030002093012 1104 78-79 -0.04291672176813677 1104 80-81 -0.1335193355873585 1104 82-83 0.6160772839145601 1104 84-85 0.30880628629575924 1104 86-87 0.8437838927273056 1104 88-89 0.9843314309237918 1104 90-91 0.5026658615754052 1104 92-93 1.1562391446753466 1104 94-95 0.8264300430009115 1104 96-97 0.35774772785075726 1104 98-99 0.45968441763947254 1104 100-101 0.2178070119509492 1105 1 0.39623840271320887 1105 2 0.18566169271791821 1105 3 0.6480887784199432 1105 4 0.7915196009407239 1105 5 0.7804978760886669 1105 6 0.6951283011868838 1105 7 0.605668426487739 1105 8 0.9873792092591742 1105 9 0.8173584553589066 1105 10-11 0.7784195625336707 1105 12-13 1.337253851686988 1105 14-15 1.1077954117780422 1105 16-17 1.0189123055744531 1105 18-19 1.4240254426912529 1105 20-21 1.4521758045943542 1105 22-23 0.7574686878543204 1105 24-25 1.0476038164532824 1105 26-27 1.309364202553489 1105 28-29 1.3963160943484212 1105 30-31 1.4087258766569235 1105 32-33 1.4778886753079803 1105 34-35 1.6454455041252132 1105 36-37 2.1671938539085858 1105 38-39 2.311362398900691 1105 40-41 2.3466480201654534 1105 42-43 2.1115596660854337 1105 44-45 1.9696441021385596 1105 46-47 2.190498132057755 1105 48-49 2.769555070916219 1105 50-51 2.762537490278497 1105 52-53 2.692173725215852 1105 54-55 2.978390576892359 1105 56-57 2.3315868372707627 1105 58-59 1.817162464358816 1105 60-61 1.969549376645773 1105 62-63 1.8105751283979927 1105 64-65 2.2777995289599886 1105 66-67 2.2886258328627918 1105 68-69 2.089627259996675 1105 70-71 1.5288875191316045 1105 72-73 1.9006310135084625 1105 74-75 1.6187101342265429 1105 76-77 1.5402253362799172 1105 78-79 1.6191040137561217 1105 80-81 1.1240119633171801 1105 82-83 0.823970476402824 1105 84-85 1.1545613723677448 1105 86-87 1.404132093040296 1105 88-89 0.46966006942144034 1105 90-91 0.2136889445175001 1105 92-93 0.7851026032042974 1105 94-95 0.6026428755517692 1105 96-97 1.174111186379708 1105 98-99 1.4114156382967522 1105 100-101 1.5491841637506347 1106 1 0.13207173604654443 1106 2 0.3220505816068062 1106 3 0.09919988953105374 1106 4 0.1284640453851651 1106 5 0.08410898719977666 1106 6 0.31346163452021614 1106 7 -0.0496093512900444 1106 8 0.12376809814806222 1106 9 -0.26153043352998395 1106 10-11 0.27466956253367414 1106 12-13 0.33280940724254293 1106 14-15 -0.1583156993330732 1106 16-17 0.16432897224111542 1106 18-19 0.35319210935792 1106 20-21 0.49953691570546965 1106 22-23 0.5191353545209907 1106 24-25 0.5594093720088367 1106 26-27 0.6347808692201511 1106 28-29 0.7747883165706497 1106 30-31 -0.12474634556530617 1106 32-33 0.16011089753019547 1106 34-35 0.016973281902991744 1106 36-37 0.3381660761308183 1106 38-39 0.7292790655673613 1106 40-41 1.0487313534987877 1106 42-43 0.7661429994187685 1106 44-45 0.8593663243607779 1106 46-47 0.5061925765022011 1106 48-49 0.827610626471774 1106 50-51 0.9226763791673847 1106 52-53 0.7088403918825179 1106 54-55 0.488390576892364 1106 56-57 0.863253503937429 1106 58-59 0.8548013532477086 1106 60-61 0.970799376645779 1106 62-63 0.23349179506465845 1106 64-65 0.2673828622933172 1106 66-67 0.541959166196122 1106 68-69 0.9682383711077804 1106 70-71 0.5723597413538251 1106 72-73 1.0095199023973542 1106 74-75 0.9566268008932113 1106 76-77 0.7474475585021416 1106 78-79 0.9525762359783414 1106 80-81 0.8320675188727336 1106 82-83 0.6277204764028212 1106 84-85 0.2165058168121874 1106 86-87 0.6955209819291852 1106 88-89 0.971326736088109 1106 90-91 0.4529945000730571 1106 92-93 0.31107482542651965 1106 94-95 0.6970873199962178 1106 96-97 0.7192500752685973 1106 98-99 1.1309989716300848 1106 100-101 1.2636286081950807 1107 1 -0.8366638961373631 1107 2 -0.777029878163308 1107 3 -1.0350529840321663 1107 4 -0.5170531959941442 1107 5 -0.307385265673787 1107 6 -0.6752739976636946 1107 7 -0.605011650140618 1107 8 -0.27416293633469735 1107 9 -0.481300548472511 1107 10-11 -0.3159051501100052 1107 12-13 -0.5945469145965419 1107 14-15 -1.2541777682985895 1107 16-17 -0.8899239013220992 1107 18-19 -0.4427849021363315 1107 20-21 -0.7385090613060257 1107 22-23 -0.35546234662843545 1107 24-25 -0.5871423521290922 1107 26-27 -0.5328053376763933 1107 28-29 -0.7936024880270622 1107 30-31 -0.6516428972894417 1107 32-33 -0.5085097921249755 1107 34-35 -0.973831315798158 1107 36-37 -1.15045461352436 1107 38-39 -1.22290484247862 1107 40-41 -1.4483950832828256 1107 42-43 -0.8117880350639908 1107 44-45 -0.7746566641449704 1107 46-47 -1.4343821361414797 1107 48-49 -1.0802054654822513 1107 50-51 -1.7201971840510062 1107 52-53 -1.6526538609910446 1107 54-55 -1.6060921817283287 1107 56-57 -1.8715740822694613 1107 58-59 -1.8843940490511457 1107 60-61 -1.6928787842737627 1107 62-63 -1.9998415382686723 1107 64-65 -1.7119274825342714 1107 66-67 -1.4435580751831907 1107 68-69 -1.3623363415359009 1107 70-71 -1.257295431059969 1107 72-73 -0.8955375688670166 1107 74-75 -1.1580858427849492 1107 76-77 -1.8631271541415408 1107 78-79 -1.7946651433320007 1107 80-81 -2.034369262736462 1107 82-83 -1.6328542362408562 1107 84-85 -1.8675171716935601 1107 86-87 -1.7714905123236875 1107 88-89 -1.8616617696590154 1107 90-91 -1.5053963045246483 1107 92-93 -0.7069711515849733 1107 94-95 -0.4707287719578055 1107 96-97 -1.1502901546164601 1107 98-99 -0.9087711433124426 1107 100-101 0.0424791829077229 1108 1 0.3204405303727853 1108 2 0.22658958869900658 1108 3 0.1528169108076476 1108 4 0.19740021559793064 1108 5 0.5480806184054501 1108 6 0.3484970955131246 1108 7 0.46840483310712244 1108 8 0.5602219988572799 1108 9 0.15719297072533323 1108 10-11 0.47722275402303893 1108 12-13 0.2793342299375823 1108 14-15 0.22452118009955058 1108 16-17 0.4858892559290595 1108 18-19 0.1833339533295515 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2316 54-55 -2.9851229366211562 2316 56-57 -3.024043793359862 2316 58-59 -3.0220454935991405 2316 60-61 -2.2798312539848524 2316 62-63 -2.8917334301605635 2316 64-65 -2.91910362419317 2316 66-67 -3.067230022993069 2316 68-69 -2.5748246919552855 2316 70-71 -2.654126745132661 2316 72-73 -2.3600296471521958 2316 74-75 -2.6275173432509327 2316 76-77 -2.8134533423987627 2316 78-79 -2.5017480883459804 2316 80-81 -2.562887436082221 2316 82-83 -2.1561533974710514 2316 84-85 -1.8360167057103318 2316 86-87 -1.9503348739266713 2316 88-89 -1.9557903810290078 2316 90-91 -2.148266761188207 2316 92-93 -2.162889138537441 2316 94-95 -2.1146243917154948 2316 96-97 -2.41002920401068 2316 98-99 -2.434586613955503 2316 100-101 -2.6960110314445593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2006.0 3 408.0 4 286.0 5 258.0 6 300.0 7 366.0 8 411.0 9 526.0 10 571.0 11 776.0 12 955.0 13 1257.0 14 1392.0 15 1713.0 16 1972.0 17 2532.0 18 2242.0 19 1362.0 20 1331.0 21 1344.0 22 1434.0 23 1513.0 24 1646.0 25 1929.0 26 2098.0 27 2455.0 28 2877.0 29 3243.0 30 3857.0 31 4474.0 32 5268.0 33 6613.0 34 8414.0 35 11825.0 36 16518.0 37 24225.0 38 22995.0 39 3850.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61656485586451 20.052301468935788 15.137461608133968 21.193672067065737 2 16.590021632947387 22.628879304480336 38.608688553585765 22.172410508986516 3 19.013595721226473 26.485118197035888 32.37183412947917 22.12945195225847 4 13.528571428571428 19.13469387755102 36.73741496598639 30.599319727891157 5 13.242882622843682 36.91505439296363 34.34108949310387 15.500973491088812 6 28.1074167057879 36.11400268065506 18.845549364194884 16.933031249362156 7 24.45751313675842 34.82874567780228 23.23436521549729 17.47937596994201 8 24.364141386634717 33.08302924677305 21.939164533522142 20.613664833070093 9 25.452616401648598 17.318443353238028 22.991280928220682 34.237659316892696 10-11 23.697175910173527 27.1772711806737 28.067710105478056 21.05784280367472 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32.079698063803455 20.22795228207987 38-39 19.818858611544783 27.645025599770932 32.349452212624676 20.186663576059612 40-41 20.080757828738882 27.547619858929362 32.09084403857293 20.280778273758816 42-43 19.949212625264163 27.704683345831345 31.440793510123388 20.905310518781103 44-45 19.75074228483966 27.872071341157863 31.286070270767784 21.091116103234693 46-47 20.149518309686304 27.92406133455148 30.867724362689287 21.05869599307293 48-49 20.236223962684253 27.917594388998946 30.68320143480144 21.162980213515368 50-51 19.896009137557748 28.435534189126987 30.314188786034542 21.354267887280724 52-53 20.176880118374665 28.353323832448023 30.197472946342728 21.27232310283458 54-55 19.75528349680723 28.248425775350388 30.20772605934154 21.788564668500847 56-57 19.934758600002727 28.47476923496448 29.82254611892069 21.767926046112105 58-59 20.087238030328848 27.83404327824161 30.178224569773388 21.90049412165616 60-61 19.899240042375048 27.616335512264577 30.70181115853513 21.782613286825246 62-63 19.844353997504754 27.541774896201908 30.63117419671528 21.98269690957806 64-65 19.797793804238488 27.542666189282734 30.54575213544064 22.11378787103814 66-67 19.735096627665488 27.722438011315003 30.01578916856216 22.526676192457348 68-69 19.613607298149883 27.4198993946628 30.234120555887117 22.732372751300193 70-71 19.75309484022781 27.481158135252194 30.108106264706887 22.657640759813116 72-73 20.024006574527796 27.373292003832866 29.889890299502476 22.71281112213686 74-75 19.933440401539873 27.724868807348834 29.6647128423881 22.676977948723195 76-77 19.95922653139148 27.38811160202913 29.74042709867452 22.91223476790487 78-79 20.124956541301103 27.283882446775877 29.88731415015236 22.70384686177066 80-81 20.098649113896435 27.250198559463872 29.710631538401227 22.940520788238462 82-83 20.25425202245927 26.936464267329402 29.718304577422778 23.090979132788554 84-85 20.397302678230513 26.629915998472697 29.555991927125945 23.416789396170838 86-87 20.48755045271081 26.5064224937275 29.671307407003383 23.33471964655831 88-89 20.581486923703707 26.271917413583257 29.710517972023442 23.436077690689594 90-91 20.815516500603557 26.242063410874916 29.573555387332828 23.3688647011887 92-93 20.894825140487754 25.982391292487318 29.587675268699876 23.535108298325056 94-95 20.857816139384038 25.694584170267333 29.431885224377886 24.015714465970746 96-97 21.309552228625993 25.35313576373495 29.334310950448454 24.003001057190602 98-99 21.551236146632565 25.30980392156863 29.21227621483376 23.926683716965048 100-101 22.03611718140071 24.764508749740045 29.590928771354445 23.608445297504797 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 663.0 1 439.5 2 215.5 3 218.5 4 194.0 5 194.0 6 239.5 7 344.0 8 499.5 9 575.5 10 521.0 11 446.0 12 433.5 13 453.5 14 484.5 15 470.5 16 467.0 17 526.5 18 577.5 19 587.0 20 615.5 21 709.5 22 783.0 23 863.0 24 963.5 25 1094.0 26 1298.0 27 1498.5 28 1669.5 29 1816.5 30 2046.0 31 2351.0 32 2547.5 33 2700.0 34 2985.5 35 3289.0 36 3593.5 37 3887.5 38 4010.5 39 4038.5 40 4074.5 41 4229.5 42 4371.0 43 4538.0 44 4679.0 45 4782.5 46 5007.0 47 5309.5 48 5774.0 49 5913.5 50 5534.0 51 5075.5 52 4737.0 53 4510.5 54 4139.0 55 3702.5 56 3321.0 57 2919.0 58 2516.0 59 2170.0 60 1921.0 61 1581.5 62 1253.5 63 975.5 64 754.0 65 593.0 66 425.5 67 327.5 68 256.0 69 181.5 70 138.0 71 117.5 72 99.0 73 86.5 74 69.0 75 50.5 76 35.5 77 26.5 78 21.0 79 10.5 80 6.5 81 8.5 82 8.0 83 4.5 84 2.0 85 2.0 86 1.5 87 1.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27437264423240176 2 0.16774763149852287 3 0.19491323983836462 4 0.16638935108153077 5 0.23837821318211144 6 0.1806512954599477 7 0.2234371285951985 8 0.2424530544330877 9 0.1432985839926653 10-11 0.20034636150633298 12-13 0.19966722129783696 14-15 0.1633332201432986 16-17 0.2825223267343543 18-19 0.22309755849095045 20-21 0.18880097796190023 22-23 0.2404156338075996 24-25 0.21902271723997419 26-27 0.34160752487351015 28-29 0.31240449590818026 30-31 0.2865971679853306 32-33 0.1837074263981799 34-35 0.35043634758395875 36-37 0.40171143332541004 38-39 0.38303507759176886 40-41 0.34636150633298246 42-43 0.37692281571530445 44-45 0.3867703487384971 46-47 0.38982647967672923 48-49 0.4105402560358586 50-51 0.4057862745763863 52-53 0.4020510034296581 54-55 0.39763659207443375 56-57 0.3796393765492886 58-59 0.3531529084179429 60-61 0.31274406601242827 62-63 0.3843933580087609 64-65 0.4159733777038269 66-67 0.4248022004142755 68-69 0.4295561818737479 70-71 0.4278583313525077 72-73 0.4200482189548032 74-75 0.4139359570783388 76-77 0.39458046113620154 78-79 0.3762436755068084 80-81 0.3826955074875208 82-83 0.39356175082345746 84-85 0.39458046113620154 86-87 0.3891473394682332 88-89 0.3983157322829298 90-91 0.416992088016571 92-93 0.41631294780807504 94-95 0.42683962103976364 96-97 0.4278583313525077 98-99 0.42106692926754724 100-101 0.3962783116574417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 147245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.82621155152293 #Duplication Level Percentage of deduplicated Percentage of total 1 88.12050539851501 65.93723578976402 2 7.443978421304485 11.140094082750021 3 1.823752888362409 4.093935583269198 4 0.8361302376394695 2.502578321849443 5 0.46838899212646723 1.7523886906629822 6 0.32456528356009356 1.457159434196855 7 0.22369935088030787 1.1717002467035793 8 0.18175561219162156 1.0880067110821467 9 0.10485899862517854 0.7061581452787614 >10 0.4444024175026185 5.2420098730005495 >50 0.016977170998931937 0.9594783571216469 >100 0.006990599823089621 1.2292382379011753 >500 0.0019973142351684633 0.9759180174461611 >1k 0.0019973142351684633 1.7440985089734609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.8007063058168359 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.7844069408129308 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 701 0.4760772861557268 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 605 0.4108798261401066 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 466 0.3164793371591565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.19491323983836462 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 242 0.16435193045604266 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.13718632211620088 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.11681211586131958 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 157 0.10662501273387892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.791402084960441E-4 0.0 12-13 0.0 0.0 0.0 6.791402084960441E-4 0.0 14-15 0.0 0.0 0.0 6.791402084960441E-4 0.0 16-17 0.0 0.0 0.0 0.001018710312744066 0.0 18-19 0.0 0.0 0.0 0.002037420625488132 0.0 20-21 0.0 0.0 0.0 0.0037352711467282425 0.0 22-23 0.0 0.0 0.0 0.011545383544432748 0.0 24-25 0.0 0.0 0.0 0.017997215525145167 0.0 26-27 0.0 0.0 0.0 0.03056130938232198 0.0 28-29 0.0 0.0 0.0 0.07232843220482868 0.0 30-31 0.0 0.0 0.0 0.16537064076878671 0.0 32-33 0.0 0.0 0.0 0.32598730007810117 0.0 34-35 0.0 0.0 0.0 0.6105470474379435 0.0 36-37 0.0 0.0 0.0 0.9779619002343033 0.0 38-39 0.0 0.0 0.0 1.3946144181466265 0.0 40-41 0.0 0.0 0.0 1.8299432917925906 0.0 42-43 0.0 0.0 0.0 2.2618764643960745 0.0 44-45 0.0 0.0 0.0 2.8153757343203503 0.0 46-47 0.0 0.0 0.0 3.3990967435227004 0.0 48-49 0.0 0.0 0.0 3.9240721246901424 0.0 50-51 0.0 0.0 0.0 4.425617168664471 0.0 52-53 0.0 0.0 0.0 4.938368026078984 0.0 54-55 0.0 0.0 0.0 5.580834663316242 0.0 56-57 0.0 0.0 0.0 6.324832761723657 0.0 58-59 0.0 0.0 0.0 7.028422017725559 0.0 60-61 0.0 0.0 0.0 7.729973853101973 0.0 62-63 0.0 0.0 0.0 8.450202044212027 0.0 64-65 0.0 0.0 0.0 9.264491154198785 0.0 66-67 0.0 0.0 0.0 10.194913239838364 0.0 68-69 0.0 0.0 0.0 11.12126048422697 0.0 70-71 0.0 0.0 0.0 12.079866888519135 0.0 72-73 0.0 0.0 0.0 13.038812862915549 0.0 74-75 0.0 0.0 0.0 14.031376277632518 0.0 76-77 0.0 0.0 0.0 15.078270909029168 0.0 78-79 0.0 0.0 0.0 16.2233013005535 0.0 80-81 0.0 0.0 0.0 17.382254066351997 0.0 82-83 0.0 0.0 0.0 18.543583822880233 0.0 84-85 0.0 0.0 0.0 19.73581445889504 0.0 86-87 0.0 0.0 0.0 20.995959115759447 0.0 88-89 0.0 0.0 0.0 22.26323474481307 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAGAC 55 0.002585487 34.50955 3 GGTATCA 1090 0.0 33.163887 1 TCGGAAG 30 0.003939963 31.676895 68-69 ATTAGTG 60 0.003959943 31.633753 9 CATGGGT 90 2.4158744E-5 31.63375 4 AAGATAT 40 4.495014E-4 29.676851 18-19 TACAGTA 80 4.5021228E-4 29.656641 7 CCCTAAT 65 0.0057894457 29.27015 1 GAGTACC 70 0.008313333 27.179426 1 GTGTGCC 70 0.008410727 27.114647 9 GTATCAA 1440 0.0 25.372906 2 CAAGACT 95 0.001228893 24.974014 4 GGTGTGC 120 1.712972E-4 23.725315 8 GTACATG 710 0.0 22.777124 1 GAGTACT 1010 0.0 22.096031 12-13 ACAGTAA 110 0.002882904 21.568468 8 ACATGGG 710 0.0 21.3862 3 TACATGG 760 0.0 21.227913 2 GGGAAGC 135 3.800831E-4 21.089169 7 TATCAAC 1745 0.0 20.9381 3 >>END_MODULE