FastQCFastQC Report
Thu 26 May 2016
SRR938812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147245
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.7830486603959387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.7640327345580495No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT10170.6906855920404767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.46928588407076643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.3511154877924548No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT4770.323949879452613RNA PCR Primer, Index 31 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.262827260687969No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.19830894088084483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.16638935108153077No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2450.16638935108153077No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT2310.15688138816258615RNA PCR Primer, Index 31 (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1690.11477469523583145No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAC550.002582483534.5177763
GGTATCA9800.033.508171
AGTAGGG650.005850611329.2073525
CTACTGG856.309124E-427.9948881
TGTAACA700.00840100927.121112
AGCATAC350.008428427.11189726-27
GTATCAA13850.026.8023281
GTACATG6750.026.7921451
CATGGGG2850.024.979974
TGGGGAG1151.284261E-424.7543416
ACATGGG6550.024.6367343
TACATGG7100.024.0652122
ATGGGGA1601.8541468E-623.7309725
TATCAAC16250.022.489662
AGTACTT10550.022.0364512-13
GAGTACT10450.021.79329712-13
ATCAACG16550.021.5084343
TCAACGC16850.021.1254944
CAACGCA16900.021.0629945
AACGCAG17200.020.6885836
Produced by FastQC (version 0.11.5)