##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938812_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147245 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950565384223573 31.0 31.0 34.0 30.0 34.0 2 32.15944174674862 33.0 31.0 34.0 30.0 34.0 3 32.202961051309046 34.0 31.0 34.0 30.0 34.0 4 35.865095588984346 37.0 35.0 37.0 35.0 37.0 5 35.762137933376344 37.0 35.0 37.0 33.0 37.0 6 35.793779075690175 37.0 35.0 37.0 33.0 37.0 7 35.7169547353051 37.0 35.0 37.0 33.0 37.0 8 35.717756120751126 37.0 35.0 37.0 33.0 37.0 9 37.33070053312506 39.0 37.0 39.0 34.0 39.0 10-11 37.268674657883125 39.0 37.0 39.0 33.0 39.0 12-13 37.18874325104418 39.0 37.0 39.0 33.0 39.0 14-15 38.345587286495295 40.0 38.0 41.0 33.0 41.0 16-17 38.26322795341098 40.0 38.0 41.0 33.0 41.0 18-19 38.401558626778495 40.0 38.0 41.0 33.5 41.0 20-21 38.4205677612143 40.0 38.0 41.0 34.0 41.0 22-23 38.32202451696153 40.0 38.0 41.0 33.5 41.0 24-25 38.20904614757717 40.0 37.5 41.0 33.0 41.0 26-27 37.98443750212232 40.0 37.0 41.0 33.0 41.0 28-29 37.77021630615641 40.0 37.0 41.0 33.0 41.0 30-31 37.44188936806003 39.5 36.5 41.0 32.0 41.0 32-33 37.16126523820843 39.0 35.5 41.0 31.5 41.0 34-35 37.01737580223437 39.0 35.0 41.0 31.0 41.0 36-37 36.79839043770586 39.0 35.0 41.0 31.0 41.0 38-39 36.600747054229345 39.0 35.0 41.0 30.5 41.0 40-41 36.3558830520561 39.0 35.0 41.0 30.0 41.0 42-43 36.03323033040171 39.0 35.0 41.0 30.0 41.0 44-45 35.63676185948589 39.0 35.0 41.0 27.5 41.0 46-47 35.1126218207749 39.0 35.0 41.0 25.0 41.0 48-49 34.611467282420456 39.0 34.5 40.5 23.0 41.0 50-51 34.24981832999423 38.0 34.0 40.0 21.0 41.0 52-53 33.99366701755578 38.0 34.0 40.0 19.5 41.0 54-55 33.67972087337431 38.0 33.5 40.0 16.5 41.0 56-57 33.341267954769265 38.0 33.0 40.0 12.5 41.0 58-59 32.96181194607627 37.0 33.0 40.0 7.5 41.0 60-61 32.527620632279536 37.0 32.5 40.0 2.0 41.0 62-63 32.120510034296586 36.0 32.0 39.5 2.0 41.0 64-65 31.64830384732928 36.0 31.0 39.0 2.0 41.0 66-67 31.175089137152366 35.0 31.0 39.0 2.0 40.0 68-69 30.788892661890046 35.0 31.0 38.5 2.0 40.0 70-71 30.366735712587865 35.0 30.0 38.0 2.0 40.0 72-73 29.89205745526164 35.0 29.5 37.0 2.0 39.5 74-75 29.407137763591294 34.5 29.0 36.5 2.0 39.0 76-77 27.50933478216578 32.5 26.5 34.5 2.0 37.0 78-79 28.365567591429247 34.0 28.0 35.0 2.0 37.0 80-81 28.404866039593877 34.0 29.0 35.0 2.0 37.0 82-83 28.079690312064926 34.0 28.0 35.0 2.0 37.0 84-85 27.67027403307413 34.0 27.0 35.0 2.0 36.0 86-87 27.115042955618186 33.5 26.0 35.0 2.0 36.0 88-89 26.54860266902102 33.0 25.0 35.0 2.0 35.0 90-91 26.06697341166084 33.0 23.5 35.0 2.0 35.0 92-93 25.755336344188258 33.0 22.5 35.0 2.0 35.0 94-95 25.419800332778703 33.0 20.0 35.0 2.0 35.0 96-97 24.881310061462187 32.5 14.5 35.0 2.0 35.0 98-99 24.13500628204693 32.0 2.0 34.0 2.0 35.0 100-101 21.080012903663963 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41253697292833635 1101 2 0.24308304415255577 1101 3 0.20105602666199474 1101 4 0.1939048078635679 1101 5 0.014182146032887033 1101 6 0.12716606327062152 1101 7 0.16507492598587703 1101 8 0.11538772516427542 1101 9 0.31866131887781535 1101 10-11 0.281129931537599 1101 12-13 0.4068628982277076 1101 14-15 0.48627361265064195 1101 16-17 0.4910315905480971 1101 18-19 0.322486370046839 1101 20-21 0.6539797747237301 1101 22-23 0.31086915687506433 1101 24-25 0.2791832939886305 1101 26-27 0.8063098990883404 1101 28-29 0.24466987008866425 1101 30-31 0.27889370903124444 1101 32-33 0.6548552734491153 1101 34-35 0.9288624695000607 1101 36-37 0.7683028603526125 1101 38-39 0.8886839322205873 1101 40-41 1.1392850078103507 1101 42-43 0.9154135163792319 1101 44-45 0.8403830612525169 1101 46-47 1.132177008730899 1101 48-49 0.757629597312544 1101 50-51 0.8071014222437185 1101 52-53 0.8850733025540833 1101 54-55 1.0876791627658164 1101 56-57 1.1970664070360684 1101 58-59 1.6012422053844944 1101 60-61 1.0134629872359397 1101 62-63 0.7700166728692821 1101 64-65 1.295952040805389 1101 66-67 0.7855419550467957 1101 68-69 1.3150431393602524 1101 70-71 1.6223971537621757 1101 72-73 1.317880664064507 1101 74-75 1.6372587103183527 1101 76-77 1.4008887903456646 1101 78-79 1.8256839062468622 1101 80-81 1.6851067014761867 1101 82-83 1.828215911148174 1101 84-85 1.8340120390817631 1101 86-87 1.4703688249851545 1101 88-89 1.3614274916191995 1101 90-91 0.8291577092115148 1101 92-93 0.8849007295031832 1101 94-95 1.3274958004497357 1101 96-97 1.2356810665687163 1101 98-99 1.1708444716789188 1101 100-101 0.614529924713235 1102 1 0.40633827006431034 1102 2 -0.11707307132698475 1102 3 0.22470293018033516 1102 4 0.06528172343493566 1102 5 -0.1406132393772026 1102 6 0.09049040465844627 1102 7 0.22660279145106443 1102 8 0.1179131226273995 1102 9 0.3011557228266142 1102 10-11 0.07680231861215958 1102 12-13 0.3300678571900022 1102 14-15 0.21066091678884646 1102 16-17 -0.11061565734261336 1102 18-19 0.19110830656753564 1102 20-21 0.12582733254958356 1102 22-23 -0.21427002696238873 1102 24-25 0.17733516220879864 1102 26-27 0.008714766217998715 1102 28-29 0.18480647113325688 1102 30-31 0.5836632665127368 1102 32-33 0.011051106543298772 1102 34-35 0.28686625759625883 1102 36-37 -0.1019548449794172 1102 38-39 0.44826035419154664 1102 40-41 0.5784458871078328 1102 42-43 0.9829105031208911 1102 44-45 1.1448943394480082 1102 46-47 1.143541297314961 1102 48-49 1.018348187681596 1102 50-51 0.8017923480314693 1102 52-53 0.8480532715605662 1102 54-55 0.7586887477971018 1102 56-57 0.7026337605343258 1102 58-59 0.5533170489820378 1102 60-61 0.3951997719458049 1102 62-63 0.5711703203467593 1102 64-65 0.80642670073766 1102 66-67 0.5135451691890225 1102 68-69 0.6842964252467247 1102 70-71 0.31355252310155635 1102 72-73 0.27250960282361447 1102 74-75 0.3241381225894848 1102 76-77 0.8901271534289421 1102 78-79 0.6335241174619206 1102 80-81 0.6147973402869553 1102 82-83 0.5241064007309078 1102 84-85 1.1859032174092619 1102 86-87 1.380860703536495 1102 88-89 0.970450048010175 1102 90-91 0.046973240405911554 1102 92-93 0.3031409866345598 1102 94-95 0.5519978953817244 1102 96-97 0.21487523519639495 1102 98-99 -0.15260728748999597 1102 100-101 0.6715235538241835 1103 1 0.21355852658796692 1103 2 0.050054305939710275 1103 3 -0.11613077172147968 1103 4 0.05958734041857383 1103 5 0.0585243103795392 1103 6 0.24705829497246867 1103 7 -0.10361613822606586 1103 8 0.20244430352081366 1103 9 0.14718624029676164 1103 10-11 0.05731426023090336 1103 12-13 -0.05294850725051248 1103 14-15 -0.01844125039381339 1103 16-17 -0.1913045118317811 1103 18-19 -0.4435666160330882 1103 20-21 -0.12624586515054403 1103 22-23 0.20161343168422974 1103 24-25 0.14231194239456357 1103 26-27 0.2515276808575351 1103 28-29 -0.08551086632803617 1103 30-31 0.20415751684444672 1103 32-33 0.374966409506591 1103 34-35 0.1883202602499523 1103 36-37 0.3042536910665774 1103 38-39 0.5049277579951763 1103 40-41 0.3361305399163257 1103 42-43 0.41131607587630725 1103 44-45 0.6933242377231039 1103 46-47 0.523931611335307 1103 48-49 -0.3678194372631225 1103 50-51 -0.9430108364001981 1103 52-53 -0.7863175820440347 1103 54-55 -0.8343010797128514 1103 56-57 -1.1716751735656317 1103 58-59 -1.4791630483200393 1103 60-61 -1.2554525942638364 1103 62-63 -1.2694422404670398 1103 64-65 -0.9560124854631304 1103 66-67 -0.6110678339069544 1103 68-69 -0.6627015844834823 1103 70-71 -0.6069472557573548 1103 72-73 -0.5191478496310502 1103 74-75 -0.24408390306288297 1103 76-77 0.2403869942073591 1103 78-79 0.34485206969544535 1103 80-81 0.19703418239221548 1103 82-83 -0.7333018257175681 1103 84-85 -0.6586069550807885 1103 86-87 -0.27971536899778116 1103 88-89 -0.315043096616094 1103 90-91 -0.18610946636100323 1103 92-93 0.10596606403393949 1103 94-95 0.12820864283860445 1103 96-97 0.47709328030917675 1103 98-99 0.15762601635785956 1103 100-101 -0.4703937394089017 1104 1 -0.14052183795221396 1104 2 -0.3058901845325366 1104 3 -0.3109319316220578 1104 4 -0.04967115171067604 1104 5 -0.2726687302169779 1104 6 -0.044159600636483276 1104 7 -0.32078680931140013 1104 8 -0.7025888364957567 1104 9 -0.16940448049859924 1104 10-11 -0.31369858152551444 1104 12-13 -0.3373312744418939 1104 14-15 0.1298603237569722 1104 16-17 -0.17343997164950764 1104 18-19 -0.19584499217227602 1104 20-21 -0.30970692563107605 1104 22-23 -0.6848302302876021 1104 24-25 -0.7472385961307069 1104 26-27 -0.5717946058036034 1104 28-29 -0.34540109002315234 1104 30-31 -0.4081560705623417 1104 32-33 -0.41516407930865284 1104 34-35 -0.3695194249198863 1104 36-37 -0.2798908822557067 1104 38-39 -0.03328682361211577 1104 40-41 0.3434834810927967 1104 42-43 0.234949050192796 1104 44-45 0.23122813167505285 1104 46-47 0.43367684745792445 1104 48-49 0.5144299413615698 1104 50-51 0.9681324941714706 1104 52-53 0.8000018048656656 1104 54-55 0.787281356078374 1104 56-57 0.9053235836837388 1104 58-59 1.150134797578879 1104 60-61 0.5926211422730319 1104 62-63 0.8918046526564041 1104 64-65 0.541812700949464 1104 66-67 0.5921218926879064 1104 68-69 0.4055979184162659 1104 70-71 -0.0953482499578513 1104 72-73 0.6985924154890846 1104 74-75 0.842908640433393 1104 76-77 0.31904275228523815 1104 78-79 0.34283259993571136 1104 80-81 0.7489708021105272 1104 82-83 0.6084545951606444 1104 84-85 0.396747228846305 1104 86-87 0.7399097345647725 1104 88-89 0.6607232662670874 1104 90-91 0.6061440547657568 1104 92-93 0.5499905047961597 1104 94-95 0.8061808880747243 1104 96-97 1.7816707450979088 1104 98-99 1.351443332016519 1104 100-101 2.048972457774198 1105 1 0.5350454546925434 1105 2 0.25797914254101784 1105 3 0.41246399952034807 1105 4 0.2238030266930835 1105 5 0.4147334607063371 1105 6 0.4243458766718149 1105 7 0.388622423865435 1105 8 0.17915998979532333 1105 9 0.008705848139896943 1105 10-11 0.37389922755117055 1105 12-13 0.2479093358867459 1105 14-15 0.22400156006370509 1105 16-17 -0.03587150529583738 1105 18-19 0.1379284820061315 1105 20-21 -0.08968540763420663 1105 22-23 -0.006719901649105964 1105 24-25 -0.045800802703475085 1105 26-27 -0.24765532567840864 1105 28-29 -0.31957949377901684 1105 30-31 0.08079150377255218 1105 32-33 0.22035039643469645 1105 34-35 0.07291993345256742 1105 36-37 -0.04133454422753857 1105 38-39 0.04843265995595658 1105 40-41 0.09695570331501813 1105 42-43 0.7060464680331648 1105 44-45 0.8994108390302955 1105 46-47 0.7837355329039326 1105 48-49 0.411388079076751 1105 50-51 0.4123813204625435 1105 52-53 0.5135190192631569 1105 54-55 0.34523323401264605 1105 56-57 0.16104541466965827 1105 58-59 0.17463597128779895 1105 60-61 0.15937583710871905 1105 62-63 0.26895645234341714 1105 64-65 0.5446002596349047 1105 66-67 0.46858902883814224 1105 68-69 0.4606644285884123 1105 70-71 -0.033826340724676385 1105 72-73 0.17917731376764223 1105 74-75 -0.18873259587333635 1105 76-77 0.37621215760605153 1105 78-79 0.25191906315950163 1105 80-81 0.12758973794777262 1105 82-83 0.16048902395563758 1105 84-85 0.16185873119372118 1105 86-87 0.573634304204834 1105 88-89 1.0558719360636424 1105 90-91 0.7097238669723289 1105 92-93 0.6006556065176021 1105 94-95 0.8923017800935042 1105 96-97 1.6402877247536196 1105 98-99 1.0085247091683165 1105 100-101 2.3351618161466554 1106 1 0.24698989913699165 1106 2 0.27270136476323614 1106 3 -0.02642488936853482 1106 4 0.06713636002641721 1106 5 -0.18637765040477206 1106 6 -0.11843190110596424 1106 7 0.10334464608765614 1106 8 0.38999332312865675 1106 9 0.34120584813990007 1106 10-11 0.07667700532894628 1106 12-13 0.10665933588674648 1106 14-15 -0.1253039954918549 1106 16-17 0.1681562724819443 1106 18-19 -0.04748818466053706 1106 20-21 -0.04482429652309605 1106 22-23 0.449113431684232 1106 24-25 0.1596158639631895 1106 26-27 0.40290022987714735 1106 28-29 0.5037538395543137 1106 30-31 0.4994026148836639 1106 32-33 0.45187817421247445 1106 34-35 0.09278104456367942 1106 36-37 0.459498789105794 1106 38-39 0.31051599328929314 1106 40-41 0.47015014775946184 1106 42-43 0.9072964680331665 1106 44-45 1.0470497279191804 1106 46-47 1.1445688662372646 1106 48-49 1.012082523521201 1106 50-51 0.8240479871292195 1106 52-53 0.7132412414853775 1106 54-55 0.4398165673459786 1106 56-57 0.7672954146696611 1106 58-59 0.840885971287797 1106 60-61 0.7622925037753774 1106 62-63 1.0167342301211946 1106 64-65 1.22251692630158 1106 66-67 0.5110890288381427 1106 68-69 0.48705331747730085 1106 70-71 0.7142292148308833 1106 72-73 0.547371758212087 1106 74-75 0.8147396263488851 1106 76-77 0.8052399353838311 1106 78-79 0.5037246187150544 1106 80-81 0.31953418239221776 1106 82-83 0.9449334684000803 1106 84-85 0.9547753978603879 1106 86-87 1.2247454153159403 1106 88-89 0.7480941582858662 1106 90-91 0.5872238669723302 1106 92-93 0.5045444954064884 1106 94-95 0.0902184467601721 1106 96-97 -0.2879067196908238 1106 98-99 -0.17203084638724064 1106 100-101 0.8146062605910984 1107 1 -0.1307112502883001 1107 2 -0.20729863523676073 1107 3 -0.08159730316164371 1107 4 -0.2388406514678394 1107 5 -0.3314351216691378 1107 6 -0.16348937237033567 1107 7 -0.23297719299279862 1107 8 -0.11667334353801095 1107 9 0.24764262974909457 1107 10-11 -0.19286322455611327 1107 12-13 0.05861335887524888 1107 14-15 -0.07300514491713983 1107 16-17 -0.3581655665985224 1107 18-19 -0.20725829960306186 1107 20-21 -0.10608866433918962 1107 22-23 -0.3298520855571496 1107 24-25 -0.38854505557704044 1107 26-27 -0.11824919541021472 1107 28-29 -0.6190047811353452 1107 30-31 -0.15048244258760235 1107 32-33 -0.47191492923580114 1107 34-35 -0.13457527727540253 1107 36-37 -0.3469379925033991 1107 38-39 -0.3667253860210522 1107 40-41 -0.2582406568382325 1107 42-43 -0.16155410667947478 1107 44-45 -0.4009962490923087 1107 46-47 -0.21209780042940451 1107 48-49 0.786795167199358 1107 50-51 -0.04077959907768047 1107 52-53 -0.09055186196290066 1107 54-55 0.468437257001149 1107 56-57 0.4052264491524227 1107 58-59 0.014219304621136075 1107 60-61 0.1920626187179053 1107 62-63 0.09328595425912312 1107 64-65 0.0011376159567504374 1107 66-67 0.021893626539295497 1107 68-69 -0.07961334918936558 1107 70-71 -0.012322509307050922 1107 72-73 -0.24929490845457636 1107 74-75 0.16129135048681675 1107 76-77 -0.0026910990989286177 1107 78-79 0.21257519342769982 1107 80-81 0.45091349273704395 1107 82-83 0.33769208908973525 1107 84-85 0.3727064323431506 1107 86-87 0.34049254175272736 1107 88-89 0.4890136985157518 1107 90-91 0.8449250163976174 1107 92-93 0.6978778287398235 1107 94-95 0.7724023548061467 1107 96-97 0.2883001768609006 1107 98-99 0.2526817972909221 1107 100-101 0.14943384679799365 1108 1 0.21308918991712744 1108 2 0.16858788958593607 1108 3 0.08563184822011749 1108 4 0.019902317473224684 1108 5 0.1919202219356535 1108 6 0.25206455279474227 1108 7 0.051004220555746826 1108 8 0.16275928057546452 1108 9 0.27156045806897566 1108 10-11 0.0018543102934884814 1108 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-3.2507708199088263 2316 94-95 -2.7687905622488422 2316 96-97 -2.665474287258391 2316 98-99 -2.596805621162016 2316 100-101 -3.152150496165657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 8.0 6 23.0 7 59.0 8 124.0 9 244.0 10 384.0 11 548.0 12 799.0 13 1053.0 14 1163.0 15 1549.0 16 1938.0 17 2530.0 18 2502.0 19 1517.0 20 1355.0 21 1449.0 22 1458.0 23 1624.0 24 1730.0 25 1801.0 26 2121.0 27 2188.0 28 2693.0 29 3206.0 30 3940.0 31 4627.0 32 5426.0 33 6771.0 34 8986.0 35 12538.0 36 17607.0 37 27656.0 38 23390.0 39 2233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.723166149322005 21.18730599575233 15.48562326417252 21.603904590753146 2 16.454208971442156 22.63710142959014 38.57312642194981 22.335563177017896 3 18.861081870352134 26.83622533872118 32.43913205881354 21.863560732113143 4 13.399436313626948 19.92461543685694 35.559102176644366 31.116846072871745 5 12.768168910748495 37.73929702042092 34.55591635983207 14.93661770899852 6 28.243979299384687 36.785020578367586 18.340555004686163 16.630445117561564 7 24.328839688953785 35.28812523345445 21.995993072769874 18.387042004821897 8 24.643281605487452 34.03307412815376 21.44860606472206 19.875038201636727 9 25.00797989744983 17.184963835783897 22.18207748989779 35.62497877686848 10-11 23.638833237121805 27.4763149852287 27.682434038507246 21.202417739142245 12-13 25.241943699276714 25.787293286699036 27.975143468369048 20.9956195456552 14-15 22.550511053006893 27.130972189208464 27.593466671194268 22.725050086590375 16-17 20.163744040423246 30.2209967264775 28.681014927805926 20.934244305293326 18-19 19.92427586675269 27.538456314306085 31.926041631294783 20.61122618764644 20-21 20.96471866616863 26.51668987062379 31.910081836395122 20.608509626812456 22-23 20.843831709056335 27.41451322625556 31.60345003225916 20.138205032428946 24-25 20.78651494622889 27.623442641321066 31.674527741273867 19.915514671176172 26-27 20.33162754213259 27.627025980773347 32.30465181824664 19.73669465884742 28-29 19.916805324459236 27.632517233182792 32.409929029848215 20.040748412509764 30-31 19.909674352270027 27.090223776698704 32.52775985602228 20.472342015008998 32-33 19.590138884172635 27.84440897823356 31.948453258175153 20.616998879418656 34-35 20.57559087204564 27.525468622656884 31.725753871230644 20.17318663406683 36-37 19.908916782133833 27.586464531203305 32.25609259108322 20.248526095579646 38-39 19.71154172694609 27.40557355448241 32.39399311286346 20.488891605708037 40-41 19.967603031867206 27.427395473933004 32.15076475861881 20.45423673558097 42-43 20.22895060344886 27.375252144501722 31.74745478378397 20.648342468265447 44-45 19.719237717499084 27.915947895301613 31.342959210008015 21.02185517719129 46-47 20.32571675003141 27.67437832992064 30.717955509373862 21.281949410674084 48-49 20.254204769650272 27.85997344534733 30.30897798544588 21.576843799556517 50-51 19.55210703249686 28.15138035247377 30.672009236306835 21.624503378722537 52-53 19.90220380997657 28.369384359401 30.603755645352983 21.12465618526945 54-55 19.41933512173588 27.986349281809233 30.25943155964549 22.3348840368094 56-57 20.085233543304412 28.520977299353106 29.48231658658336 21.911472570759123 58-59 19.80610547047438 28.431865258582633 30.069272301266597 21.692756969676388 60-61 19.625514459189635 28.89766506839081 29.7211393488271 21.755681123592453 62-63 19.283363951549646 28.9265740083603 29.709291072271444 22.080770967818612 64-65 19.08613652261907 29.04722126009386 29.460144115509735 22.406498101777334 66-67 19.355802699719842 29.213685372272693 28.743017234060613 22.687494693946856 68-69 19.080573741544647 28.525101192578305 29.13531281410448 23.259012251772567 70-71 19.061428231858468 28.33271078814221 29.22204489116778 23.383816088831537 72-73 19.379265849434617 27.94967571055044 28.997249482155592 23.67380895785935 74-75 19.113042887704164 28.28652925396448 28.621345376753034 23.979082481578324 76-77 19.161703096384436 27.807319468181618 28.891886473832102 24.13909096160184 78-79 18.924635384610152 27.717230521258042 28.927117727669966 24.431016366461844 80-81 18.929464018907392 27.641194208252966 28.72239276303279 24.70694900980685 82-83 18.91254962018697 27.320730626479268 28.67223340928462 25.094486344049145 84-85 18.85700702910116 26.960507996875954 28.65054840571836 25.531936568304523 86-87 18.930357306754868 26.96542510699486 28.618533991902723 25.48568359434754 88-89 18.919185998964096 26.59712946051304 28.520022898890495 25.963661641632363 90-91 18.688473180442312 26.4364211502277 28.531400834446842 26.34370483488315 92-93 18.836357215379863 26.269232467087058 28.688677675566833 26.205732641966257 94-95 18.823453507601304 25.986709180988083 28.492065917572475 26.69777139383814 96-97 18.559204047675642 25.798159530034976 28.40673707086828 27.2358993514211 98-99 18.616591395293558 25.711908723555975 28.32761723657849 27.34388264457197 100-101 18.465179772075903 25.068594558617786 29.07933877562109 27.386886893685226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 456.0 1 302.0 2 156.0 3 160.5 4 137.5 5 140.0 6 231.0 7 369.5 8 485.5 9 532.0 10 486.5 11 401.0 12 347.5 13 372.0 14 421.0 15 425.0 16 429.5 17 435.5 18 452.5 19 510.0 20 571.0 21 632.5 22 686.5 23 782.0 24 882.5 25 1016.0 26 1165.5 27 1371.5 28 1571.5 29 1714.0 30 1950.5 31 2236.5 32 2543.0 33 2783.0 34 3053.5 35 3368.5 36 3613.5 37 3902.0 38 4027.0 39 3960.0 40 4021.5 41 4286.0 42 4548.0 43 4750.0 44 4954.0 45 5049.5 46 5149.5 47 5424.5 48 5850.5 49 5944.0 50 5792.5 51 5630.0 52 5141.0 53 4681.5 54 4343.5 55 3890.5 56 3365.0 57 2878.0 58 2560.0 59 2228.0 60 1827.5 61 1438.5 62 1157.0 63 901.5 64 666.5 65 500.5 66 349.5 67 277.0 68 215.5 69 154.5 70 106.0 71 76.0 72 67.0 73 44.5 74 29.0 75 29.5 76 24.0 77 14.0 78 9.5 79 6.0 80 2.5 81 2.5 82 2.5 83 1.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.231586811097151 2 0.0 3 0.0 4 0.0 5 0.029203028965329895 6 0.002037420625488132 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002037420625488132 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.39570104248022E-4 26-27 0.001018710312744066 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0033957010424802204 36-37 0.011545383544432748 38-39 0.009507962918944617 40-41 0.006112261876464397 42-43 0.005433121667968352 44-45 0.002037420625488132 46-47 3.39570104248022E-4 48-49 0.001018710312744066 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0016978505212401102 58-59 0.0 60-61 0.002037420625488132 62-63 0.001018710312744066 64-65 0.001358280416992088 66-67 0.00509355156372033 68-69 6.79140208496044E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.06078304866039593 78-79 0.14024245305443309 80-81 6.79140208496044E-4 82-83 0.001018710312744066 84-85 0.0 86-87 0.10764372304662298 88-89 0.3483989269584706 90-91 0.3809976569662807 92-93 3.39570104248022E-4 94-95 3.39570104248022E-4 96-97 0.0 98-99 0.0 100-101 0.002037420625488132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 147245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.98305944942962 #Duplication Level Percentage of deduplicated Percentage of total 1 87.71007538571149 64.01349646189335 2 7.707657873663684 11.250569056189219 3 1.8474942903990825 4.045073568860341 4 0.8723564458285773 2.5466896938800074 5 0.5355489981612498 1.9543002185442482 6 0.3397102360789071 1.4875855413195989 7 0.20782249882016543 1.0617265250424806 8 0.16286088148811656 0.9508868316506978 9 0.11190440800830988 0.7350413456091337 >10 0.46859970788829824 5.746885750687516 >50 0.018983783474722543 0.9880764106153158 >100 0.011989757984035291 1.9360463986595304 >500 0.001998292997339215 0.8808828811980085 >1k 0.0029974394960088227 2.4027393158505284 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1153 0.7830486603959387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1125 0.7640327345580495 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1017 0.6906855920404767 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.46928588407076643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 517 0.3511154877924548 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 477 0.323949879452613 RNA PCR Primer, Index 31 (95% over 22bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.262827260687969 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.19830894088084483 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 245 0.16638935108153077 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 245 0.16638935108153077 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 231 0.15688138816258615 RNA PCR Primer, Index 31 (95% over 24bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 169 0.11477469523583145 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.3957010424802203E-4 0.0 18-19 0.0 0.0 0.0 0.0013582804169920881 0.0 20-21 0.0 0.0 0.0 0.0030561309382321983 0.0 22-23 0.0 0.0 0.0 0.010866243335936703 0.0 24-25 0.0 0.0 0.0 0.01867635573364121 0.0 26-27 0.0 0.0 0.0 0.03225915990356209 0.0 28-29 0.0 0.0 0.0 0.07674284356005298 0.0 30-31 0.0 0.0 0.0 0.1711433325410031 0.0 32-33 0.0 0.0 0.0 0.33447655268430165 0.0 34-35 0.0 0.0 0.0 0.6261672722333527 0.0 36-37 0.0 0.0 0.0 0.9962986858636966 0.0 38-39 0.0 0.0 0.0 1.4160073347142519 0.0 40-41 0.0 0.0 0.0 1.8564297599239363 0.0 42-43 0.0 0.0 0.0 2.292098203674149 0.0 44-45 0.0 0.0 0.0 2.857821997351353 0.0 46-47 0.0 0.0 0.0 3.4483344086386634 0.0 48-49 0.0 0.0 0.0 3.9787429114740736 0.0 50-51 0.0 0.0 0.0 4.484362796699379 0.0 52-53 0.0 0.0 0.0 5.003905056198852 0.0 54-55 0.0 0.0 0.0 5.654521375938062 0.0 56-57 0.0 0.0 0.0 6.411423138306903 0.0 58-59 0.0 0.0 0.0 7.118747665455533 0.0 60-61 0.0 0.0 0.0 7.834561445210364 0.0 62-63 0.0 0.0 0.0 8.570749431220076 0.0 64-65 0.0 0.0 0.0 9.39386736391728 0.0 66-67 0.0 0.0 0.0 10.33651397330979 0.0 68-69 0.0 0.0 0.0 11.281877143536283 0.0 70-71 0.0 0.0 0.0 12.255764202519611 0.0 72-73 0.0 0.0 0.0 13.224897280043464 0.0 74-75 0.0 0.0 0.0 14.238174471119564 0.0 76-77 0.0 0.0 0.0 15.304085028354104 0.0 78-79 0.0 0.0 0.0 16.46337736425685 0.0 80-81 0.0 0.0 0.0 17.63455465380828 0.0 82-83 0.0 0.0 0.0 18.817956467112637 0.0 84-85 0.0 0.0 0.0 20.025467757818603 0.0 86-87 0.0 0.0 0.0 21.29749736833169 0.0 88-89 0.0 0.0 0.0 22.580053652076472 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACAC 55 0.0025824835 34.517776 3 GGTATCA 980 0.0 33.50817 1 AGTAGGG 65 0.0058506113 29.207352 5 CTACTGG 85 6.309124E-4 27.994888 1 TGTAACA 70 0.008401009 27.12111 2 AGCATAC 35 0.0084284 27.111897 26-27 GTATCAA 1385 0.0 26.802328 1 GTACATG 675 0.0 26.792145 1 CATGGGG 285 0.0 24.97997 4 TGGGGAG 115 1.284261E-4 24.754341 6 ACATGGG 655 0.0 24.636734 3 TACATGG 710 0.0 24.065212 2 ATGGGGA 160 1.8541468E-6 23.730972 5 TATCAAC 1625 0.0 22.48966 2 AGTACTT 1055 0.0 22.03645 12-13 GAGTACT 1045 0.0 21.793297 12-13 ATCAACG 1655 0.0 21.508434 3 TCAACGC 1685 0.0 21.125494 4 CAACGCA 1690 0.0 21.062994 5 AACGCAG 1720 0.0 20.688583 6 >>END_MODULE