FastQCFastQC Report
Thu 26 May 2016
SRR938811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998123
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72170.7230571783237136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60240.6035328311240198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.5364068356304784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47950.48040171401721027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.3325241478254684No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30160.3021671677739116No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24400.24445884926006115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23200.23243628290300894No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21640.2168069466388411No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.20869171434783088No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20030.20067667010979612No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18570.1860492143753826No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18530.18564846216348085No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17970.18003793119685652No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16460.16490953519756585No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16310.1634067144029343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.1375581967352721No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC12520.12543544232524448No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11750.117720962246136No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11400.11421438039199577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.10950554190215034No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC10890.1091047896902486No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT10300.10319369456469793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT52300.020.82016612-13
GTACTTT57450.019.03666714-15
CGCCGTT650.00750999218.29237494-95
AGACCCG1300.007685718618.2180735
AGTACTT55250.018.20894212-13
GTACACG1851.5493184E-417.9298381
GTACATG44950.017.6050321
TACATGG42950.017.4248752
TAAGGTG3551.6425474E-917.345665
ACATGGG40550.016.9376413
CGTATCC1002.735064E-416.64605994-95
GTGCTAT2304.358805E-516.4820861
ACTTTTT67650.016.41339516-17
CGTATCA1601.5816113E-716.34880894-95
GAGTGTC2901.065051E-616.3252587
GTGTAGC5001.8189894E-1216.111241
GGAGTGT5350.015.928576
CATGGGG14700.015.7889974
CATATAG2104.088701E-415.7889973
TACCTGG6150.015.40392
Produced by FastQC (version 0.11.5)