##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938811_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998123 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58771413943973 33.0 31.0 34.0 30.0 34.0 2 31.62568541151742 33.0 31.0 34.0 30.0 34.0 3 31.627973706647378 33.0 31.0 34.0 28.0 34.0 4 35.168781803445064 37.0 35.0 37.0 33.0 37.0 5 35.21546142108738 37.0 35.0 37.0 33.0 37.0 6 35.328507608781685 37.0 35.0 37.0 33.0 37.0 7 35.263858261957694 37.0 35.0 37.0 33.0 37.0 8 35.246371439191364 37.0 35.0 37.0 32.0 37.0 9 36.798734224138705 39.0 37.0 39.0 33.0 39.0 10-11 36.708381131383604 39.0 37.0 39.0 32.0 39.0 12-13 36.612539737086514 39.0 37.0 39.0 32.0 39.0 14-15 37.70793980301025 40.0 37.0 41.0 32.0 41.0 16-17 37.66343376517724 40.0 37.0 41.0 32.0 41.0 18-19 37.663602081106234 40.0 37.0 41.0 32.0 41.0 20-21 37.56017344555731 40.0 37.0 41.0 32.0 41.0 22-23 37.46109547620884 40.0 37.0 41.0 32.0 41.0 24-25 37.431402742948514 40.0 37.0 41.0 32.0 41.0 26-27 37.30170780555102 40.0 37.0 41.0 31.5 41.0 28-29 37.179360659958746 40.0 37.0 41.0 31.0 41.0 30-31 36.97377477525315 39.5 36.0 41.0 31.0 41.0 32-33 36.71622335123026 39.0 35.5 41.0 30.0 41.0 34-35 36.346344588793166 39.0 35.0 41.0 30.0 41.0 36-37 36.04660648036364 39.0 35.0 41.0 29.5 41.0 38-39 35.8405391920635 39.0 35.0 41.0 29.0 41.0 40-41 35.67550241803866 39.0 35.0 41.0 27.5 41.0 42-43 35.381849731946865 38.5 35.0 40.0 26.5 41.0 44-45 35.25200651623096 39.0 35.0 41.0 25.5 41.0 46-47 34.97061334124151 39.0 35.0 41.0 24.0 41.0 48-49 34.64729647548448 39.0 35.0 41.0 23.0 41.0 50-51 33.43633850737835 38.0 32.5 40.0 20.0 40.5 52-53 33.52019240113693 38.0 33.5 39.5 20.0 40.5 54-55 34.0608582308994 38.0 34.0 40.0 20.0 41.0 56-57 33.95289007467015 38.0 34.0 40.0 18.5 41.0 58-59 33.73591130552046 38.0 34.0 40.0 16.5 41.0 60-61 33.4187264495458 38.0 33.0 40.0 13.0 41.0 62-63 33.061195864637924 37.0 33.0 40.0 9.5 41.0 64-65 32.660820860755635 36.5 32.5 39.5 7.5 41.0 66-67 32.22256024558095 36.0 32.0 39.0 4.5 41.0 68-69 31.799419009480793 35.5 31.5 39.0 2.0 40.5 70-71 31.342163741342503 35.0 31.0 38.5 2.0 40.0 72-73 30.888281804947887 35.0 31.0 37.0 2.0 40.0 74-75 30.425065848597818 35.0 30.5 37.0 2.0 39.0 76-77 29.976179288524563 35.0 30.0 36.5 2.0 39.0 78-79 29.538121053216887 34.5 29.5 36.0 2.0 38.5 80-81 29.064562684158165 34.0 29.0 35.5 2.0 37.0 82-83 28.567773210315764 34.0 29.0 35.0 2.0 37.0 84-85 28.124115965667556 34.0 28.5 35.0 2.0 36.0 86-87 27.750879400634993 34.0 27.0 35.0 2.0 36.0 88-89 27.330081563093927 34.0 26.0 35.0 2.0 36.0 90-91 26.88581717884469 34.0 25.5 35.0 2.0 35.0 92-93 26.421692516854137 34.0 25.0 35.0 2.0 35.0 94-95 25.895498350403706 33.0 23.0 35.0 2.0 35.0 96-97 25.2967675326588 33.0 18.5 35.0 2.0 35.0 98-99 24.615097538078974 33.0 2.0 35.0 2.0 35.0 100-101 22.529830992773437 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.014735127217278432 1101 2 -0.14513907332142395 1101 3 -0.1007067971460529 1101 4 -0.026236039074191808 1101 5 -0.09560704809462095 1101 6 -0.028925306792380923 1101 7 -0.04755205430373621 1101 8 0.10924351499873808 1101 9 -0.009242914906629096 1101 10-11 0.08398064621213308 1101 12-13 0.09347210252049365 1101 14-15 0.0055532337221464445 1101 16-17 0.008393152847013141 1101 18-19 0.1310213749864957 1101 20-21 0.08181268781802231 1101 22-23 0.2551180209877302 1101 24-25 0.11525110211951528 1101 26-27 0.01298815375549367 1101 28-29 -0.18073650827314225 1101 30-31 -0.14418056348064567 1101 32-33 0.015197559628660429 1101 34-35 0.057290676884115044 1101 36-37 0.21773419600324218 1101 38-39 0.1864454087855023 1101 40-41 0.09744258710579601 1101 42-43 0.18080634001807994 1101 44-45 0.16027593449793898 1101 46-47 0.42989847664080116 1101 48-49 0.48443356583255337 1101 50-51 0.14268913937673489 1101 52-53 0.13985004649636323 1101 54-55 -0.06195224731084892 1101 56-57 -0.0285347809531018 1101 58-59 -0.039491575677736535 1101 60-61 -0.14762934736211264 1101 62-63 -0.16657666430139528 1101 64-65 -0.29295444014380223 1101 66-67 -0.12220356800464316 1101 68-69 -0.24106515296308118 1101 70-71 -0.535606696062807 1101 72-73 -0.6863405061026064 1101 74-75 -0.6725488378878914 1101 76-77 -0.7017829459754559 1101 78-79 -0.8481641351839606 1101 80-81 -0.8742900607772306 1101 82-83 -1.0348661454598194 1101 84-85 -0.9738477000436347 1101 86-87 -1.1985509587493617 1101 88-89 -0.9440037277523778 1101 90-91 -0.899978301014098 1101 92-93 -1.4965620479421275 1101 94-95 -1.5687807791895345 1101 96-97 -1.718054519789785 1101 98-99 -1.9463024415966075 1101 100-101 -1.681891809277154 1102 1 0.17788096766339123 1102 2 0.15039931672447793 1102 3 0.061680346740750736 1102 4 0.13866902901882838 1102 5 0.18592509218836994 1102 6 0.04872965697985876 1102 7 0.1460163327073758 1102 8 0.0519939322818459 1102 9 0.22316955047784148 1102 10-11 0.13918649007238315 1102 12-13 0.3211871692857855 1102 14-15 0.0759614598895908 1102 16-17 0.27769664569630237 1102 18-19 0.22158307124232834 1102 20-21 0.20533026586893044 1102 22-23 0.18997562312726046 1102 24-25 0.0841872198692144 1102 26-27 0.050534149734410505 1102 28-29 0.19191508878311936 1102 30-31 0.20287865752766265 1102 32-33 -0.04260444902048022 1102 34-35 0.2529437012382729 1102 36-37 0.23950108174354057 1102 38-39 0.27241402719906915 1102 40-41 0.32584877901809506 1102 42-43 0.1633794356897127 1102 44-45 0.35555909427943533 1102 46-47 0.24494011011449146 1102 48-49 0.4159173392098907 1102 50-51 0.3833198296009357 1102 52-53 0.29507758433635445 1102 54-55 0.24993012627133737 1102 56-57 0.069878239228224 1102 58-59 -0.05229043099429731 1102 60-61 0.09754700216939227 1102 62-63 0.06937500198592517 1102 64-65 0.1016294147934147 1102 66-67 0.028268483510849762 1102 68-69 -0.09667393582412842 1102 70-71 0.010325267823237283 1102 72-73 -0.19816313233074823 1102 74-75 -0.42648279809425205 1102 76-77 -0.36892794711350163 1102 78-79 -0.5482715381656398 1102 80-81 -0.7005521050851087 1102 82-83 -0.9275580008522546 1102 84-85 -1.041038578614252 1102 86-87 -0.8166290381090207 1102 88-89 -0.6971392783764081 1102 90-91 -0.8087645999026059 1102 92-93 -1.1078616477296919 1102 94-95 -0.8592453859950417 1102 96-97 -0.7332248945858844 1102 98-99 -0.6883793971369911 1102 100-101 -0.5426075683530627 1103 1 0.0315626061435168 1103 2 0.1139718696200056 1103 3 0.1902723007637377 1103 4 0.24854621622140627 1103 5 0.0989197294238977 1103 6 0.352910966006867 1103 7 0.1747542527600885 1103 8 0.1948278696125456 1103 9 0.16845288015058202 1103 10-11 0.28831206661531894 1103 12-13 0.2460615652884286 1103 14-15 0.3584240303559483 1103 16-17 0.32676113614216007 1103 18-19 0.5607264474045692 1103 20-21 0.3850366407865664 1103 22-23 0.3870366268610539 1103 24-25 0.366519381813724 1103 26-27 0.3101615016635364 1103 28-29 0.2415778569213387 1103 30-31 0.29421152413576124 1103 32-33 0.2513340713690084 1103 34-35 0.28271688192499767 1103 36-37 0.2978039864217763 1103 38-39 0.2074088108581975 1103 40-41 0.2581427335535622 1103 42-43 0.2621181336178182 1103 44-45 0.4629550877635822 1103 46-47 0.29758397130491687 1103 48-49 0.2781121375113784 1103 50-51 0.15350455212819725 1103 52-53 0.26831066325867425 1103 54-55 0.35988738888206484 1103 56-57 0.3152075550639353 1103 58-59 0.058985787982202 1103 60-61 0.28972569387449454 1103 62-63 0.24004887086359616 1103 64-65 0.0889729245671873 1103 66-67 -0.142482028971763 1103 68-69 -0.02462551536874713 1103 70-71 -0.07814661884489382 1103 72-73 -0.028588584413622442 1103 74-75 0.019513202711078037 1103 76-77 -0.019592115427432333 1103 78-79 0.09140514021491697 1103 80-81 -0.13191375308276676 1103 82-83 0.056719764723556665 1103 84-85 0.04796180574770759 1103 86-87 -0.0301535457596529 1103 88-89 -0.15193327866925443 1103 90-91 -0.20278708507721532 1103 92-93 -0.2189857234306949 1103 94-95 -0.19564903926394805 1103 96-97 -0.29527418443140974 1103 98-99 -0.4493119782190611 1103 100-101 -0.252964521643932 1104 1 -0.06855320161560385 1104 2 0.07589950112425825 1104 3 -0.0013498026230713833 1104 4 0.11059646770590348 1104 5 0.06509137468883353 1104 6 0.035114038318596386 1104 7 0.07760516730077427 1104 8 -0.07979774206814483 1104 9 0.04319997644132911 1104 10-11 -0.016975920032727743 1104 12-13 0.10286186502615635 1104 14-15 0.25541586704430586 1104 16-17 0.2120382232364122 1104 18-19 0.059001820089825685 1104 20-21 0.18275454670979485 1104 22-23 0.08229191484770837 1104 24-25 -0.028402675022910273 1104 26-27 0.02249616337999072 1104 28-29 0.10276125326771535 1104 30-31 0.0911329678724897 1104 32-33 0.10519773611689942 1104 34-35 0.1877278382473122 1104 36-37 0.3113841492338594 1104 38-39 0.21105845832575199 1104 40-41 0.09414872830815568 1104 42-43 0.23343925036793678 1104 44-45 0.19629012415219194 1104 46-47 0.2944537556981217 1104 48-49 0.4932903338622978 1104 50-51 0.48065352405049566 1104 52-53 0.4984388465528369 1104 54-55 0.4839299879715 1104 56-57 0.27340776624279073 1104 58-59 0.22970004936711774 1104 60-61 0.34649477093206826 1104 62-63 0.3270085765756434 1104 64-65 0.4054142202516431 1104 66-67 0.3155160863137283 1104 68-69 0.38453976046943694 1104 70-71 0.4823356864107353 1104 72-73 0.33458511587135575 1104 74-75 0.35593871674992883 1104 76-77 0.356512883607504 1104 78-79 0.2191484784303448 1104 80-81 0.33803470111072187 1104 82-83 0.20783753667310023 1104 84-85 -0.0635849090586511 1104 86-87 -0.20226544784532763 1104 88-89 -0.12329753948649369 1104 90-91 -0.3163417020600825 1104 92-93 -0.20688778639832606 1104 94-95 -0.017743910796813367 1104 96-97 -0.09576523203748266 1104 98-99 0.19755723848904694 1104 100-101 0.7158650252298244 1105 1 0.12777655558371848 1105 2 0.14862788101376267 1105 3 0.13540687633309645 1105 4 0.32272432280050367 1105 5 0.20748350940833404 1105 6 0.15346746664645394 1105 7 0.27946077120241597 1105 8 0.15543105300719873 1105 9 0.22060833108753286 1105 10-11 0.14784510719238853 1105 12-13 0.27872960342585884 1105 14-15 0.2602287012689004 1105 16-17 0.12613738068595381 1105 18-19 0.06230891367825109 1105 20-21 0.24731262290469402 1105 22-23 0.2837345472366266 1105 24-25 0.3419779673526193 1105 26-27 0.13879359144212344 1105 28-29 0.16083910891590847 1105 30-31 0.07239590783107985 1105 32-33 0.2326708597537035 1105 34-35 0.24383107005386506 1105 36-37 0.3513770633955673 1105 38-39 0.3120526900105176 1105 40-41 0.08386374474951452 1105 42-43 0.3164000236100435 1105 44-45 0.37370069312286347 1105 46-47 0.3626892712482075 1105 48-49 0.3237201342409506 1105 50-51 0.3734508801470895 1105 52-53 0.2947708180766284 1105 54-55 0.34879364094257426 1105 56-57 0.30426216333759015 1105 58-59 0.11720406283021845 1105 60-61 0.10710249228147717 1105 62-63 0.17337099506082154 1105 64-65 0.26141559313281704 1105 66-67 0.17118586410627046 1105 68-69 0.05395052872930961 1105 70-71 0.10660302180339087 1105 72-73 0.11861374708668748 1105 74-75 0.13288372400183945 1105 76-77 0.15863974225610278 1105 78-79 -0.12155855537131899 1105 80-81 0.031182474053892406 1105 82-83 -0.06773791089723247 1105 84-85 -0.16904377244590663 1105 86-87 0.12108737154329674 1105 88-89 0.04540685926905752 1105 90-91 0.11186325581239132 1105 92-93 0.27428879490613056 1105 94-95 0.4445179109279245 1105 96-97 0.5865683337999208 1105 98-99 0.4352273262870128 1105 100-101 0.53784662419352 1106 1 0.11461874536192695 1106 2 0.20467088182093818 1106 3 0.0870320387851109 1106 4 0.22085754308650962 1106 5 0.10671080520053522 1106 6 0.10021532185165682 1106 7 0.016216783800345524 1106 8 0.22014529804157235 1106 9 0.23298037670898708 1106 10-11 0.19348368545090722 1106 12-13 0.17851731852088903 1106 14-15 0.2343755471841007 1106 16-17 0.1444074781678637 1106 18-19 0.14630971871228127 1106 20-21 0.1238967307621337 1106 22-23 0.11903657416673497 1106 24-25 -0.22864931687791312 1106 26-27 0.035405597154444024 1106 28-29 0.065309295893627 1106 30-31 -0.026447176905179504 1106 32-33 0.1618728269485672 1106 34-35 0.30264137534589963 1106 36-37 0.2955974114871509 1106 38-39 0.4359786868289177 1106 40-41 0.43000857379754365 1106 42-43 0.4294056053220672 1106 44-45 0.32442554490882713 1106 46-47 0.3483549551738676 1106 48-49 0.5646699245252975 1106 50-51 0.43824273816991877 1106 52-53 0.573636961899382 1106 54-55 0.5996575537933566 1106 56-57 0.48444526575890023 1106 58-59 0.5253108197634688 1106 60-61 0.6018874347194014 1106 62-63 0.4083627841419073 1106 64-65 0.3669585521895584 1106 66-67 0.44272908865295335 1106 68-69 0.6083722209707929 1106 70-71 0.6345474444805141 1106 72-73 0.4267688917037695 1106 74-75 0.29363842199632373 1106 76-77 0.2277923797060275 1106 78-79 0.37748867828766564 1106 80-81 0.6789822261205387 1106 82-83 0.47880900695718154 1106 84-85 0.4809510890389994 1106 86-87 0.6879164366975274 1106 88-89 0.754942123399438 1106 90-91 0.7281981670980713 1106 92-93 0.8289106751316275 1106 94-95 0.791641198892453 1106 96-97 0.8400453956826333 1106 98-99 0.9304977513492645 1106 100-101 1.0599486622570709 1107 1 0.0921246375380349 1107 2 -0.016183747304783225 1107 3 0.07269591902669958 1107 4 0.14813536305057795 1107 5 -0.0306586377026008 1107 6 0.0829482904540555 1107 7 -0.05502396524354225 1107 8 0.08117088281275642 1107 9 0.10095729151978361 1107 10-11 0.06922772910785824 1107 12-13 0.14842435366733753 1107 14-15 0.23705671362729674 1107 16-17 0.26706005970248015 1107 18-19 0.1644138491463707 1107 20-21 0.2481202042545263 1107 22-23 0.31688109739835824 1107 24-25 0.18494097532929032 1107 26-27 0.213581488063177 1107 28-29 -0.10526454295096244 1107 30-31 -0.006807299892699348 1107 32-33 0.14172493945211784 1107 34-35 0.05314911957699309 1107 36-37 0.09268023849626417 1107 38-39 0.26692621690750684 1107 40-41 0.282226513490194 1107 42-43 0.4238994958836173 1107 44-45 0.2581030189571507 1107 46-47 0.456091518190064 1107 48-49 0.4977595122273968 1107 50-51 0.4643557550599269 1107 52-53 0.2539034703763434 1107 54-55 0.11184927290296542 1107 56-57 0.0722181882676054 1107 58-59 -0.0736340733466605 1107 60-61 0.07855995539927108 1107 62-63 0.1205237244192503 1107 64-65 0.07377419976447186 1107 66-67 0.07943662691082665 1107 68-69 0.07262085003474539 1107 70-71 0.1607856002214021 1107 72-73 -0.057050388625359716 1107 74-75 0.2407611336859965 1107 76-77 0.30575920885794616 1107 78-79 0.23981464209644443 1107 80-81 -0.07838312713845141 1107 82-83 -0.04656968450579413 1107 84-85 0.08813970364009194 1107 86-87 -0.05541259753939798 1107 88-89 -0.2057743950353803 1107 90-91 -0.09516115606582431 1107 92-93 -0.05001935041799754 1107 94-95 -0.19261793118485926 1107 96-97 -0.24755978751532126 1107 98-99 -0.22941478990662034 1107 100-101 0.052124022790088986 1108 1 0.10518417212177056 1108 2 0.07958339892580213 1108 3 -0.04104083054939167 1108 4 0.03370995552526779 1108 5 0.13401591420161196 1108 6 0.061673753113609564 1108 7 -0.04111141023933129 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96-97 -3.1392120686817613 2316 98-99 -3.185370040534803 2316 100-101 -3.3666062016583638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5659.0 3 1170.0 4 1060.0 5 1111.0 6 1216.0 7 1594.0 8 1879.0 9 2434.0 10 2915.0 11 3888.0 12 5464.0 13 7098.0 14 7887.0 15 8052.0 16 9917.0 17 12446.0 18 10867.0 19 7465.0 20 7623.0 21 7628.0 22 8203.0 23 9135.0 24 10294.0 25 11714.0 26 13582.0 27 15585.0 28 18470.0 29 21889.0 30 25925.0 31 30775.0 32 37220.0 33 47148.0 34 61173.0 35 85522.0 36 121761.0 37 180294.0 38 163777.0 39 28280.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10055193176116 20.095032614149524 15.053988961364775 20.750426492724536 2 15.299084868998369 22.983778362793032 38.42828130876269 23.288855459445905 3 20.759360133492713 25.732545266383745 31.780230403285977 21.727864196837572 4 12.697271225556422 17.97899986058748 38.866706584784374 30.457022329071727 5 12.353937273281757 38.64673284966775 34.81679962119996 14.182530255850534 6 28.276771037966565 37.34344305015435 18.09034598885459 16.289439923024496 7 24.748818263097334 35.09768624133493 22.743774581931753 17.409720913635983 8 24.07213430910379 32.485698734712656 21.28916371553237 22.153003240651177 9 24.345466555869525 18.830435266369225 23.561800647341318 33.26229753041993 10-11 23.83366636746219 26.53382388834511 28.22345211801186 21.409057626180843 12-13 26.58024640000481 25.060507384933224 27.859058515615644 20.50018769944632 14-15 22.923604024787043 26.97796150430325 28.613206658259976 21.485227812649732 16-17 19.86534315243769 29.548728157354255 29.54070928895692 21.045219401251146 18-19 19.496518693602937 29.34702199447883 31.214828580994254 19.941630730923983 20-21 21.28792321379345 27.35849434879583 30.335914592887754 21.01766784452297 22-23 20.54642074959158 27.12483451327478 30.821811714329684 21.50693302280396 24-25 21.67281088200449 27.40014208654645 30.717316459427874 20.209730572021183 26-27 21.133185863779847 26.41792376773345 32.30139741774528 20.147492950741423 28-29 19.826691599280096 28.72382451584441 31.45314356472871 19.99634032014679 30-31 20.32974111247399 27.727587384001374 30.748351219248114 21.194320284276518 32-33 20.400338217980185 27.70731012954831 30.909955727927997 20.98239592454351 34-35 21.735447332245382 27.17460990570209 30.559547161013885 20.53039560103864 36-37 21.36504087260662 26.597368711127483 31.682523959586927 20.355066456678962 38-39 20.678697374459553 27.466073685820792 31.435935524906807 20.41929341481285 40-41 20.52606186589769 27.892024705307655 31.22513835988512 20.356775068909535 42-43 20.040691811112698 28.180463099582713 29.89615184094464 21.882693248359946 44-45 20.59947487064273 27.425552571065342 29.921854671935073 22.053117886356855 46-47 21.43074631988618 27.963832240459435 29.702113225057538 20.903308214596848 48-49 21.049841867329636 27.622201092003866 29.951581150525286 21.376375890141208 50-51 19.933149488554832 28.17989139434302 30.710718278093495 21.17624083900865 52-53 20.446341967993888 28.799964564049723 29.398046895252033 21.35564657270435 54-55 19.947082384181687 27.942554641439614 29.731153950299515 22.379209024079177 56-57 20.714061437019204 27.82408208777982 29.215240905413076 22.2466155697879 58-59 21.520331624567987 27.265254377977556 29.232766036653775 21.981647960800686 60-61 20.74334141531795 27.467281038316248 30.38730660695956 21.402070939406237 62-63 20.253106589497406 27.416643056603263 30.48596807382645 21.84428228007289 64-65 19.992096453472275 28.49092602381132 29.740240126859476 21.77673739585693 66-67 20.209409693013786 27.804651635764664 28.978761513682006 23.00717715753954 68-69 20.025847989254004 27.56531475741959 29.503108404473206 22.9057288488532 70-71 20.99199900113379 27.35992210454665 28.911789641999103 22.736289252320454 72-73 20.69864027426086 26.572192324924615 30.102998847178103 22.626168553636422 74-75 20.129397480710683 28.221345902933265 29.13003777323175 22.519218843124307 76-77 20.376966122772927 27.253419655767154 29.23945969967322 23.1301545217867 78-79 20.47967082065401 27.329624128548197 29.552731890708273 22.63797316008952 80-81 21.067173330126433 27.073721646730043 29.122498403678005 22.73660661946552 82-83 20.738172725090497 26.58924485298595 29.810686102470758 22.861896319452796 84-85 20.667476534303294 26.857327758867445 29.59546205733219 22.87973364949707 86-87 20.65573192949963 26.907304699688968 29.530081431749323 22.906881939062078 88-89 20.730006367358854 26.755641907791585 28.971583458960737 23.542768265888824 90-91 20.825063386843222 26.24127219672095 29.291290544550492 23.642373871885336 92-93 21.47589588188806 26.433385852138592 29.13877548965816 22.951942776315192 94-95 21.277987051591772 26.030619698273195 29.185667850827006 23.505725399308027 96-97 20.86881711336335 25.66435653981357 29.826612117669978 23.640214229153106 98-99 21.32234907764569 26.0720698318384 29.008140034158846 23.597441056357063 100-101 21.582188140459095 25.20197739544285 29.524937891825076 23.69089657227298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2621.0 1 1694.0 2 865.5 3 925.0 4 764.0 5 780.0 6 1183.0 7 1861.0 8 2581.5 9 2846.5 10 2494.5 11 2150.0 12 2187.0 13 2247.5 14 2338.0 15 2506.0 16 2604.0 17 2698.0 18 2844.5 19 2994.0 20 3230.0 21 3551.0 22 4002.0 23 4635.0 24 5310.5 25 6199.5 26 7747.0 27 9644.5 28 10701.0 29 11766.5 30 13417.5 31 15221.0 32 17329.0 33 19175.0 34 20932.5 35 22982.5 36 25571.5 37 27808.0 38 28619.0 39 28353.5 40 27981.0 41 28161.5 42 28167.5 43 28671.0 44 30325.5 45 32688.0 46 37531.0 47 46171.0 48 56597.0 49 56339.5 50 43754.0 51 33172.5 52 28552.0 53 26656.5 54 24780.0 55 22546.0 56 20096.5 57 17666.0 58 16168.0 59 14524.5 60 11987.0 61 9662.5 62 7772.0 63 5784.5 64 4042.5 65 2962.5 66 2171.5 67 1716.0 68 1368.5 69 1014.5 70 773.5 71 544.5 72 409.5 73 328.5 74 262.5 75 206.5 76 144.0 77 104.0 78 66.5 79 38.0 80 30.0 81 25.5 82 21.0 83 17.5 84 10.0 85 8.5 86 7.0 87 4.0 88 3.0 89 2.5 90 1.5 91 2.5 92 3.0 93 2.0 94 2.0 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16260520997913083 2 0.09998767686948402 3 0.09167206847252293 4 0.10840347331942055 5 0.12934277639128644 6 0.04037578534910026 7 0.043982555256215916 8 0.04849101764011049 9 0.05049477869961919 10-11 0.04783979529577016 12-13 0.051847317414787555 14-15 0.05059496675259462 16-17 0.047689513216307 18-19 0.05099571896449636 20-21 0.05490305303053832 22-23 0.06877909836763604 24-25 0.08450862268477932 26-27 0.08566078529399683 28-29 0.07694442468513399 30-31 0.06447101208969234 32-33 0.05425183068619799 34-35 0.29846020981382054 36-37 0.47403977265327013 38-39 0.4507460503364816 40-41 0.4255487550131597 42-43 0.4565569574090568 44-45 0.4662251045211863 46-47 0.46502284788548104 48-49 0.4820548168913049 50-51 0.4721361996467369 52-53 0.47904917530204194 54-55 0.4700823445607405 56-57 0.44633777600556246 58-59 0.4235950879801388 60-61 0.39439027053779946 62-63 0.45580554701174103 64-65 0.4907210834736801 66-67 0.4911719297120695 68-69 0.5018419573539534 70-71 0.5006397007182481 72-73 0.49172296400343446 74-75 0.4836077317124242 76-77 0.4647222837265547 78-79 0.43872348397942945 80-81 0.4440835448136151 82-83 0.45700780364744625 84-85 0.4553547007733516 86-87 0.4661750104946985 88-89 0.47844804698418936 90-91 0.4826559452091576 92-93 0.4837079197653997 94-95 0.48826647617578195 96-97 0.48966910891743803 98-99 0.48315688547403474 100-101 0.4703829087196668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 998123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.433603135028186 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81894077426541 46.99156573357535 2 10.68618148637393 12.27491813034572 3 3.1058948318250224 5.351481934505204 4 1.2851536963051013 2.952440294444069 5 0.7403842326586358 2.1261467092974233 6 0.4397013910173205 1.515218111376518 7 0.3115705131828617 1.2526232041905083 8 0.2409261877739885 1.1069807242757228 9 0.177754867565084 0.9188192267147276 >10 1.0604642360880556 11.524506779930894 >50 0.08839962969235474 3.4853724551409297 >100 0.03852664819535843 3.824909008709344 >500 0.002091944590941039 0.9008089958357824 >1k 0.0034865743182350656 3.9124166190151204 >5k 5.229861477352597E-4 1.8617920726427004 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7217 0.7230571783237136 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6024 0.6035328311240198 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5354 0.5364068356304784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4795 0.48040171401721027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3319 0.3325241478254684 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3016 0.3021671677739116 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2440 0.24445884926006115 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2320 0.23243628290300894 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2164 0.2168069466388411 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2083 0.20869171434783088 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2003 0.20067667010979612 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1857 0.1860492143753826 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1853 0.18564846216348085 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1797 0.18003793119685652 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1646 0.16490953519756585 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1631 0.1634067144029343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1373 0.1375581967352721 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1252 0.12543544232524448 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1175 0.117720962246136 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1140 0.11421438039199577 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1093 0.10950554190215034 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1089 0.1091047896902486 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1030 0.10319369456469793 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.003761059508698E-4 0.0 5 0.0 0.0 0.0 2.003761059508698E-4 0.0 6 0.0 0.0 0.0 3.005641589263047E-4 0.0 7 0.0 0.0 0.0 3.005641589263047E-4 0.0 8 0.0 0.0 0.0 3.005641589263047E-4 0.0 9 0.0 0.0 0.0 3.005641589263047E-4 0.0 10-11 0.0 0.0 0.0 4.50846238389457E-4 0.0 12-13 0.0 0.0 0.0 5.009402648771744E-4 0.0 14-15 2.003761059508698E-4 0.0 0.0 5.510342913648919E-4 0.0 16-17 2.003761059508698E-4 0.0 0.0 6.011283178526094E-4 0.0 18-19 2.003761059508698E-4 0.0 0.0 7.013163708280443E-4 0.0 20-21 2.003761059508698E-4 0.0 0.0 0.001252350662192936 0.0 22-23 2.003761059508698E-4 0.0 0.0 0.0033562997746770687 0.0 24-25 2.003761059508698E-4 0.0 0.0 0.006562317469890985 0.0 26-27 2.003761059508698E-4 0.0 0.0 0.012573600648417078 0.0 28-29 3.005641589263047E-4 0.0 0.0 0.035216100620865366 0.0 30-31 3.005641589263047E-4 0.0 0.0 0.08315608396961097 0.0 32-33 3.005641589263047E-4 0.0 0.0 0.18875429180571934 0.0 34-35 3.005641589263047E-4 0.0 0.0 0.36227999955917256 0.0 36-37 3.005641589263047E-4 0.0 0.0 0.6088928919582055 0.0 38-39 3.005641589263047E-4 0.0 0.0 0.9226317798507799 0.0 40-41 3.005641589263047E-4 0.0 0.0 1.2365209498228174 0.0 42-43 3.5065818541402214E-4 0.0 0.0 1.5595272326156195 0.0 44-45 6.011283178526094E-4 0.0 0.0 1.980918183430299 0.0 46-47 6.011283178526094E-4 0.0 0.0 2.4741940622548526 0.0 48-49 6.011283178526094E-4 0.0 0.0 2.931151771875811 0.0 50-51 6.011283178526094E-4 0.0 0.0 3.378391240358152 0.0 52-53 6.011283178526094E-4 0.0 0.0 3.811704569476908 0.0 54-55 6.011283178526094E-4 0.0 0.0 4.298167660699132 0.0 56-57 6.011283178526094E-4 0.0 0.0 4.923892145557211 0.0 58-59 6.011283178526094E-4 0.0 0.0 5.54170177423023 0.0 60-61 6.011283178526094E-4 0.0 0.0 6.1792484493394095 0.0 62-63 6.011283178526094E-4 0.0 0.0 6.7964068556680886 0.0 64-65 7.013163708280443E-4 0.0 0.0 7.445625438948907 0.0 66-67 7.013163708280443E-4 0.0 0.0 8.194080288701894 0.0 68-69 7.013163708280443E-4 0.0 0.0 9.017325520001041 0.0 70-71 7.013163708280443E-4 0.0 0.0 9.883300955894214 0.0 72-73 7.013163708280443E-4 0.0 0.0 10.723578156199185 0.0 74-75 7.013163708280443E-4 0.0 0.0 11.625120350898637 0.0 76-77 7.013163708280443E-4 0.0 0.0 12.54549789955747 0.0 78-79 7.013163708280443E-4 0.0 0.0 13.60218129428938 0.0 80-81 0.0010018805297543488 0.0 0.0 14.712565485416125 0.0 82-83 0.0010018805297543488 0.0 0.0 15.797802475246037 0.0 84-85 0.0010018805297543488 0.0 0.0 16.908737700664147 0.0 86-87 0.0010018805297543488 0.0 0.0 18.018520763473038 0.0 88-89 0.0010018805297543488 0.0 0.0 19.181553776438374 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 5230 0.0 20.820166 12-13 GTACTTT 5745 0.0 19.036667 14-15 CGCCGTT 65 0.007509992 18.292374 94-95 AGACCCG 130 0.0076857186 18.218073 5 AGTACTT 5525 0.0 18.208942 12-13 GTACACG 185 1.5493184E-4 17.929838 1 GTACATG 4495 0.0 17.605032 1 TACATGG 4295 0.0 17.424875 2 TAAGGTG 355 1.6425474E-9 17.34566 5 ACATGGG 4055 0.0 16.937641 3 CGTATCC 100 2.735064E-4 16.646059 94-95 GTGCTAT 230 4.358805E-5 16.482086 1 ACTTTTT 6765 0.0 16.413395 16-17 CGTATCA 160 1.5816113E-7 16.348808 94-95 GAGTGTC 290 1.065051E-6 16.325258 7 GTGTAGC 500 1.8189894E-12 16.11124 1 GGAGTGT 535 0.0 15.92857 6 CATGGGG 1470 0.0 15.788997 4 CATATAG 210 4.088701E-4 15.788997 3 TACCTGG 615 0.0 15.4039 2 >>END_MODULE