FastQCFastQC Report
Thu 26 May 2016
SRR938811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998123
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76920.7706465034870452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.5572459506493688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.5360060834185766No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47050.4713847892494212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.33242395977249295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29020.290745729734712No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT24490.24536054173684005No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23790.23834737802855963No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23770.23814700192260874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22930.2297312054726722No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21850.21891089575132522No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20280.20318137143418197No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20070.2010774223216978No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18750.18785259932894044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.18614940242835804No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16500.16531028740946757No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16250.1628055860850817No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.13795894894717384No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13400.13425199098708274No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT12640.1266376989609497RNA PCR Primer, Index 23 (95% over 23bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11930.11952434719969382No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC11900.11922378304076751No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC11880.11902340693481667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.11671908171638165No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT10030.1004886171343612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG47800.021.7200761
TACATGG46050.021.2286762
ACATGGG43050.020.5033633
GCTACCG600.00479068719.7730762-63
ATGGGAG8750.019.5244925
GAGTACT52850.018.76664412-13
CGGAGAT1300.007448896318.3172131
CATGGGG14300.018.2520624
CATGGGA23850.017.9076864
CCCTATA2403.1171148E-617.7957632
AGTACTT54950.017.18583712-13
GTACTTT58300.016.93090214-15
GTGTTAA3201.5550722E-716.371011
GTATAGT1750.002131780616.3284871
TCTATAC2406.1955885E-515.8184563
TAAGGCG2154.8209756E-415.4505845
GTGTAGC4351.3624231E-915.3275061
GTACTGC5651.8189894E-1215.1185236
ACTAGTA2205.7406933E-415.0994348
TAAGGTG3152.45929E-615.0651955
Produced by FastQC (version 0.11.5)