##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938811_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998123 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907427240931227 31.0 31.0 34.0 30.0 34.0 2 32.1117697918994 33.0 31.0 34.0 30.0 34.0 3 32.18848478594322 34.0 31.0 34.0 30.0 34.0 4 35.8409214094856 37.0 35.0 37.0 35.0 37.0 5 35.74869129355801 37.0 35.0 37.0 33.0 37.0 6 35.800104796703415 37.0 35.0 37.0 33.0 37.0 7 35.73752733881496 37.0 35.0 37.0 33.0 37.0 8 35.724848540710916 37.0 35.0 37.0 33.0 37.0 9 37.33454293709293 39.0 37.0 39.0 34.0 39.0 10-11 37.268955830093084 39.0 37.0 39.0 33.0 39.0 12-13 37.21073955815065 39.0 37.0 39.0 33.0 39.0 14-15 38.34180256341152 40.0 38.0 41.0 33.0 41.0 16-17 38.29710516639733 40.0 38.0 41.0 33.0 41.0 18-19 38.37053799982567 40.0 38.0 41.0 33.5 41.0 20-21 38.432502807770184 40.0 38.0 41.0 34.0 41.0 22-23 38.365439429809754 40.0 38.0 41.0 33.5 41.0 24-25 38.263185499181965 40.0 38.0 41.0 33.0 41.0 26-27 38.054117077754945 40.0 37.0 41.0 33.0 41.0 28-29 37.87635892570354 40.0 37.0 41.0 33.0 41.0 30-31 37.65057813515969 40.0 37.0 41.0 32.5 41.0 32-33 37.37009516863152 40.0 36.0 41.0 32.0 41.0 34-35 37.253173707048134 40.0 36.0 41.0 31.0 41.0 36-37 37.10654197929514 40.0 36.0 41.0 31.0 41.0 38-39 36.955406297620634 40.0 35.0 41.0 31.0 41.0 40-41 36.80728527446016 39.5 35.0 41.0 31.0 41.0 42-43 36.59468973262814 39.0 35.0 41.0 30.0 41.0 44-45 36.301316571204154 39.0 35.0 41.0 30.0 41.0 46-47 35.9254155049027 39.0 35.0 41.0 28.5 41.0 48-49 35.56402267055263 39.0 35.0 41.0 27.0 41.0 50-51 35.263695957311874 39.0 35.0 40.5 26.5 41.0 52-53 35.05025382643221 39.0 35.0 40.0 26.0 41.0 54-55 34.81879487798598 38.0 34.0 40.0 25.5 41.0 56-57 34.51424123079019 38.0 34.0 40.0 24.0 41.0 58-59 34.18328452505352 38.0 34.0 40.0 23.0 41.0 60-61 33.7635116112944 37.5 33.0 40.0 22.0 41.0 62-63 33.372321848108896 37.0 33.0 40.0 21.5 41.0 64-65 32.98095926053202 36.0 32.5 39.0 19.5 41.0 66-67 32.530026860417 36.0 32.0 39.0 17.5 40.0 68-69 32.05312471509022 35.0 31.5 38.5 13.5 40.0 70-71 31.528743952398653 35.0 31.0 37.5 9.5 40.0 72-73 31.16650603182173 35.0 31.0 37.0 7.5 39.0 74-75 30.757914104774663 35.0 31.0 37.0 4.5 39.0 76-77 28.717071443098696 32.5 28.0 35.0 2.0 37.0 78-79 29.731888254253235 34.0 29.5 35.5 2.0 37.0 80-81 29.760130765446743 34.0 30.0 35.0 2.0 37.0 82-83 29.441436576454002 34.0 30.0 35.0 2.0 37.0 84-85 29.061478895887582 34.0 29.0 35.0 2.0 36.0 86-87 28.579919508918238 34.0 29.0 35.0 2.0 36.0 88-89 28.065867633548173 34.0 28.5 35.0 2.0 35.5 90-91 27.6556997484278 33.5 27.0 35.0 2.0 35.0 92-93 27.39268256517483 33.0 27.0 35.0 2.0 35.0 94-95 27.019492086646636 33.0 26.0 35.0 2.0 35.0 96-97 26.514848871331488 33.0 25.0 35.0 2.0 35.0 98-99 25.81858197837341 33.0 23.5 35.0 2.0 35.0 100-101 22.95551049319573 29.5 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04793576212275852 1101 2 0.028656444994048513 1101 3 0.09566546934991749 1101 4 0.058363310939789415 1101 5 0.054368895416210705 1101 6 0.09897844431605307 1101 7 0.06249034450961233 1101 8 0.07971622369335307 1101 9 0.07742396112479355 1101 10-11 0.18767270618880616 1101 12-13 0.17841614145557827 1101 14-15 0.1583644021973214 1101 16-17 0.04727784369545418 1101 18-19 0.0775746621472706 1101 20-21 0.07484292872149467 1101 22-23 0.018400851920567618 1101 24-25 0.0847068229975605 1101 26-27 0.12866532601398717 1101 28-29 0.10656512100299409 1101 30-31 0.2570448557713547 1101 32-33 0.1526785053645554 1101 34-35 0.1556723140859262 1101 36-37 0.1466431678656761 1101 38-39 0.2684721203619276 1101 40-41 0.36272487091139993 1101 42-43 0.16184989903731406 1101 44-45 -0.01100271173051226 1101 46-47 -0.015095835434472349 1101 48-49 0.06846252070185699 1101 50-51 -0.07486147653124675 1101 52-53 0.05214423951179725 1101 54-55 0.12671833440196423 1101 56-57 -0.17270319528903855 1101 58-59 -0.08547886374347513 1101 60-61 -0.019549011452120624 1101 62-63 -0.0983513855213971 1101 64-65 -0.215048053159407 1101 66-67 -0.2880819277841482 1101 68-69 -0.3513255640186195 1101 70-71 -0.3978502837771565 1101 72-73 -0.3128852293592317 1101 74-75 -0.161646687779335 1101 76-77 -0.1540330776180454 1101 78-79 -0.15697357341244 1101 80-81 -0.11578375975993183 1101 82-83 -0.18152937451680629 1101 84-85 -0.20332107049978987 1101 86-87 -0.3081120746922217 1101 88-89 -0.22683652453754988 1101 90-91 -0.2713346871603761 1101 92-93 -0.13489157388009332 1101 94-95 -0.268047862663618 1101 96-97 -0.5741768217395737 1101 98-99 -0.23400040076851525 1101 100-101 0.516969940924664 1102 1 0.10434886289146661 1102 2 0.13029926013763315 1102 3 0.21245139363200138 1102 4 0.10784929402085908 1102 5 0.0808497762629159 1102 6 0.14518662116822156 1102 7 0.06086388876058635 1102 8 0.16535172689884803 1102 9 0.17321058720118998 1102 10-11 0.31546980701957494 1102 12-13 0.21916881586095371 1102 14-15 0.28794325447910296 1102 16-17 0.451965402968618 1102 18-19 0.31544322271022196 1102 20-21 0.28191789268340983 1102 22-23 0.2690143292165743 1102 24-25 0.19838979689839675 1102 26-27 0.13763217097434932 1102 28-29 0.05579468835627921 1102 30-31 0.1724821969192618 1102 32-33 0.28530814763912815 1102 34-35 0.284728969751491 1102 36-37 0.2897303752933169 1102 38-39 0.3960825107113024 1102 40-41 0.27823452532511794 1102 42-43 0.4095305399462248 1102 44-45 0.3316371018198083 1102 46-47 0.2279259344149338 1102 48-49 0.4225402776154752 1102 50-51 0.29035845063388166 1102 52-53 0.3306238116069409 1102 54-55 0.3746550353996483 1102 56-57 0.20475367755341978 1102 58-59 0.0572716720859745 1102 60-61 0.202248814882239 1102 62-63 -0.10720383326616911 1102 64-65 -0.02774602554285366 1102 66-67 -0.11511155962664787 1102 68-69 0.2961881636472512 1102 70-71 0.14371798942947578 1102 72-73 0.14157400966811906 1102 74-75 0.20349025694962108 1102 76-77 0.053969511433962225 1102 78-79 0.042947119087841656 1102 80-81 0.013425820680875944 1102 82-83 0.05077402823726018 1102 84-85 0.11000816113026701 1102 86-87 0.28687614180184084 1102 88-89 0.45446587294357954 1102 90-91 0.4246241867442535 1102 92-93 0.5527915802111778 1102 94-95 0.5077626386451293 1102 96-97 0.37738040294841113 1102 98-99 0.3699665153196463 1102 100-101 0.7541479690723101 1103 1 -0.08505921969144481 1103 2 -0.026170375267589918 1103 3 -0.1057842934607578 1103 4 -0.006031663589510572 1103 5 -0.05156749440111241 1103 6 -0.04086515787856371 1103 7 -0.0181224938442881 1103 8 0.07655953859809728 1103 9 0.05822458895827509 1103 10-11 -0.013458234930787683 1103 12-13 0.10480511793284819 1103 14-15 0.11650066168314765 1103 16-17 -0.007999180822288565 1103 18-19 0.07151179471403424 1103 20-21 -0.0011452433441263565 1103 22-23 -0.0674865584765243 1103 24-25 0.03304895020390575 1103 26-27 -0.05911539473324723 1103 28-29 -0.05075936940783521 1103 30-31 -0.07029042552747455 1103 32-33 0.03739468398879353 1103 34-35 -0.1047349368302477 1103 36-37 -0.03972387648207132 1103 38-39 0.002601106508947737 1103 40-41 -0.059162063004926324 1103 42-43 -0.17514462844091128 1103 44-45 -0.035390819331084344 1103 46-47 0.14231273981796022 1103 48-49 0.19631352236253719 1103 50-51 0.3597678144233214 1103 52-53 0.08402816038796601 1103 54-55 -0.05903909484414527 1103 56-57 0.09250740220383591 1103 58-59 -0.18528932249635943 1103 60-61 0.19954455395538417 1103 62-63 0.09107774847480954 1103 64-65 0.05140279574713702 1103 66-67 8.913962998491343E-4 1103 68-69 -0.10199824275146341 1103 70-71 -0.23300020004266386 1103 72-73 -0.2022103083639486 1103 74-75 -0.39756948388059854 1103 76-77 -0.34836365717281836 1103 78-79 -0.26160139288228734 1103 80-81 -0.4383664957405351 1103 82-83 -0.497930122871896 1103 84-85 -0.3616966214306814 1103 86-87 -0.19005870884608456 1103 88-89 -0.18992152730534428 1103 90-91 -0.16813630999781992 1103 92-93 -0.19036654812910925 1103 94-95 0.01935412594288266 1103 96-97 0.08380107796122616 1103 98-99 0.07664228770415349 1103 100-101 0.2881365480312361 1104 1 0.006796769953979265 1104 2 -0.10894908026435957 1104 3 0.0147091384227096 1104 4 -0.06395597981281753 1104 5 -0.04200427131222284 1104 6 -0.03837529105748416 1104 7 -0.10887149755694736 1104 8 -0.22536604923981685 1104 9 -0.010381289988558251 1104 10-11 0.025442953801807278 1104 12-13 -0.014644341063259958 1104 14-15 -0.11259092862987785 1104 16-17 -0.024391087903588016 1104 18-19 0.0914948776982385 1104 20-21 0.06258876212835673 1104 22-23 0.13212457723427207 1104 24-25 0.015100450595610937 1104 26-27 0.09333473413126825 1104 28-29 0.15773376727938881 1104 30-31 0.16729941063860565 1104 32-33 0.12619879857034988 1104 34-35 0.17998298044667393 1104 36-37 0.0871389821529931 1104 38-39 0.10401861618797881 1104 40-41 0.16255132414495677 1104 42-43 0.3136674337319576 1104 44-45 0.2208134110582307 1104 46-47 0.27677746954308446 1104 48-49 -0.04222207716968995 1104 50-51 -0.08457009445186969 1104 52-53 -0.11846340948839185 1104 54-55 -0.24889569758946806 1104 56-57 -0.022110488959576458 1104 58-59 -0.05903445459667722 1104 60-61 -0.06168028340284337 1104 62-63 0.010058867268590177 1104 64-65 0.10838134860429705 1104 66-67 -0.03528547166794738 1104 68-69 -0.18995707422846664 1104 70-71 -0.16824379371638187 1104 72-73 -0.15219327781113634 1104 74-75 -0.3016959868268856 1104 76-77 -0.13693842697651348 1104 78-79 -0.10244576769069269 1104 80-81 -0.2550706296233862 1104 82-83 -0.25878710028938556 1104 84-85 -0.27252975607423124 1104 86-87 -0.040542376545936065 1104 88-89 0.33119692586631544 1104 90-91 0.25597157287739236 1104 92-93 0.238591068501794 1104 94-95 0.46039321673843503 1104 96-97 0.4439302830885552 1104 98-99 0.38637751937643827 1104 100-101 0.7290234992216718 1105 1 -0.1608131989347612 1105 2 -0.11629842316357042 1105 3 -0.2313200242304987 1105 4 -0.06402905252016211 1105 5 -0.17366136102558016 1105 6 -0.10842987415554717 1105 7 -0.06124469980227332 1105 8 -0.05133011075375293 1105 9 -0.11339387842313897 1105 10-11 -0.0832515912098799 1105 12-13 -0.2362262544610303 1105 14-15 -0.23113091379390482 1105 16-17 -0.34128306208456394 1105 18-19 -0.38699462086798775 1105 20-21 -0.2797153531936516 1105 22-23 -0.1879056482946666 1105 24-25 -0.2356933847250744 1105 26-27 -0.24093747277520805 1105 28-29 -0.3409968712210727 1105 30-31 -0.28272992916055273 1105 32-33 -0.27357312600976513 1105 34-35 -0.11628034701869439 1105 36-37 -0.45806202315031896 1105 38-39 -0.3404643944075687 1105 40-41 -0.43926386833519615 1105 42-43 -0.3676087921734208 1105 44-45 -0.2145128142935704 1105 46-47 -0.20394406808382115 1105 48-49 -0.17817052769614605 1105 50-51 -0.22054234896632607 1105 52-53 -0.1701258135804622 1105 54-55 0.04468497578660191 1105 56-57 0.054356777789017485 1105 58-59 -0.26982928636337533 1105 60-61 -0.18467825729595688 1105 62-63 -0.16061351235519794 1105 64-65 -0.096053389717909 1105 66-67 -0.0432066638602393 1105 68-69 -0.18913279194637767 1105 70-71 -0.3127301257986268 1105 72-73 -0.15893644938806517 1105 74-75 -0.16522717005116405 1105 76-77 -0.22486205492571543 1105 78-79 -0.25627045962280093 1105 80-81 -0.14454207037359978 1105 82-83 -0.1233174974284772 1105 84-85 -0.07256684600901764 1105 86-87 -0.14357046525177353 1105 88-89 0.20250703093601174 1105 90-91 0.19714306123558245 1105 92-93 0.2357190896707344 1105 94-95 0.051161698044001724 1105 96-97 0.09262992104650891 1105 98-99 0.1681154319327689 1105 100-101 0.27711862660068576 1106 1 -0.10059087251487497 1106 2 -0.06769021138823916 1106 3 -0.11753403053802458 1106 4 -0.03277403443900795 1106 5 -0.06878426788357217 1106 6 0.026323402183088263 1106 7 0.009393824930036487 1106 8 7.005661813792585E-4 1106 9 -0.11156970556348966 1106 10-11 -0.12688936015226915 1106 12-13 0.0828123721851668 1106 14-15 0.012277870069446806 1106 16-17 -0.17011163051567024 1106 18-19 -0.1289763962677739 1106 20-21 -0.2721280144948537 1106 22-23 -0.32253313806581474 1106 24-25 -0.37069662841273043 1106 26-27 -0.21697058194082075 1106 28-29 -0.24229830722193668 1106 30-31 -0.07578051570064304 1106 32-33 0.11047842186086143 1106 34-35 -0.0011422815411705756 1106 36-37 -0.04148474523586998 1106 38-39 -0.08465466077534245 1106 40-41 0.04483403322370094 1106 42-43 -0.14707282363826124 1106 44-45 0.019081940995356206 1106 46-47 0.036741302777798523 1106 48-49 0.12027121760050363 1106 50-51 0.4955188293380104 1106 52-53 0.40028468954439234 1106 54-55 0.1430436484225126 1106 56-57 0.049619709183417626 1106 58-59 0.12166775577887279 1106 60-61 0.270770816807854 1106 62-63 0.28125596507922523 1106 64-65 0.15483407466040688 1106 66-67 -0.07214239174161463 1106 68-69 -0.08145788438612556 1106 70-71 -0.25503905332629273 1106 72-73 -0.2230624414961646 1106 74-75 -0.21084219678214566 1106 76-77 -0.13748535252496197 1106 78-79 -0.29657889899153744 1106 80-81 -0.25688339195686183 1106 82-83 -0.10813372057706161 1106 84-85 -0.079273143473376 1106 86-87 0.08761239951321187 1106 88-89 0.10397065131964212 1106 90-91 0.3870384924533461 1106 92-93 0.4025741685576456 1106 94-95 0.43318353245107133 1106 96-97 0.1287087201755277 1106 98-99 0.3040671598655216 1106 100-101 0.6330571442675463 1107 1 -0.19564847366167726 1107 2 -0.1348146909969934 1107 3 -0.17760706902647172 1107 4 -0.25855221171443077 1107 5 -0.0994278436809708 1107 6 -0.2057191727165062 1107 7 -0.21414143813312592 1107 8 -0.07752133322402699 1107 9 -0.33603046361737654 1107 10-11 -0.2253613881578076 1107 12-13 -0.1965231032412902 1107 14-15 -0.294589750066514 1107 16-17 -0.2041935959304837 1107 18-19 -0.16761448086590036 1107 20-21 -0.19456109715998338 1107 22-23 -0.43859968850422604 1107 24-25 -0.21482900082169465 1107 26-27 -0.20398596177184203 1107 28-29 -0.06786859425319136 1107 30-31 -0.3315451566799368 1107 32-33 -0.21652963069107045 1107 34-35 -0.5882756646460408 1107 36-37 -0.3487405620624173 1107 38-39 -0.25463528821621395 1107 40-41 -0.3891986221588155 1107 42-43 -0.4579368994159623 1107 44-45 -0.7145842439311139 1107 46-47 -0.6445066137934603 1107 48-49 -0.8275362964816111 1107 50-51 -0.5422150604993305 1107 52-53 -0.5496986430256854 1107 54-55 -0.7817164843631232 1107 56-57 -0.6618159582646115 1107 58-59 -0.7889969498466272 1107 60-61 -0.6428695309396986 1107 62-63 -0.5602644666338321 1107 64-65 -0.5774736881018896 1107 66-67 -0.6399154633519188 1107 68-69 -0.5530790555366707 1107 70-71 -0.589314744332377 1107 72-73 -0.5946204574649485 1107 74-75 -0.5911269169557016 1107 76-77 -0.669283933290636 1107 78-79 -0.4497733477256105 1107 80-81 -0.387765849832995 1107 82-83 -0.19549714616524128 1107 84-85 -0.42779336324915107 1107 86-87 -0.3276393106289994 1107 88-89 -0.35714631143796893 1107 90-91 -0.1132128502598988 1107 92-93 -0.13272137745777357 1107 94-95 -0.09809106265818102 1107 96-97 -0.164897376802287 1107 98-99 -0.4232062935514094 1107 100-101 0.18181763063699563 1108 1 -0.09653062160870718 1108 2 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quality scores pass #Quality Count 2 1.0 3 6.0 4 14.0 5 44.0 6 130.0 7 347.0 8 543.0 9 1051.0 10 1820.0 11 2632.0 12 3719.0 13 4947.0 14 5658.0 15 7203.0 16 9104.0 17 12129.0 18 11773.0 19 8061.0 20 7567.0 21 7589.0 22 7877.0 23 8621.0 24 9734.0 25 10865.0 26 12480.0 27 15040.0 28 17773.0 29 21599.0 30 26158.0 31 31271.0 32 38336.0 33 47758.0 34 62426.0 35 86599.0 36 128206.0 37 201090.0 38 170668.0 39 17284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63976595130556 20.93997624514368 15.550882359152912 20.869375444397846 2 15.443186861739486 23.506621929361412 37.73843504257491 23.31175616632419 3 20.66108084875311 25.834391152192666 32.06228090125165 21.442247097802575 4 12.856832274178634 18.689680530355478 38.06675129217542 30.386735903290475 5 12.1407882595632 39.275820715482965 34.72878593119285 13.854605093760988 6 28.392993649079322 37.8506456619074 17.54202638352187 16.214334305491406 7 24.712785899132673 35.28723413847793 22.247658855672096 17.752321106717307 8 24.382265512366715 32.94143106611109 20.876284786544343 21.800018634977853 9 23.975301641180497 18.777946205026836 23.12911334574997 34.11763880804269 10-11 23.85868274751709 26.8387763832714 27.96514056884773 21.337400300363782 12-13 26.538312412398067 25.040100268203417 27.781495867743754 20.640091451654758 14-15 22.676670802773813 27.17562659017624 28.269883245560028 21.87781936148992 16-17 19.887486223825267 29.828474100791503 29.223574792105 21.06046488327823 18-19 19.574350843709063 29.396542007619303 30.797372066054017 20.231735082617615 20-21 21.312203005040463 27.411701764211426 29.985582939176837 21.29051229157128 22-23 20.56495041192318 27.48884656500251 30.391344553727347 21.554858469346964 24-25 21.730187562053978 27.806392598908154 30.324969968631123 20.138449870406752 26-27 21.324880112170824 26.760947200262898 31.9550595343949 19.959113153171383 28-29 19.819050357521068 28.96827344926427 31.126624674514066 20.0860515187006 30-31 20.603172154133308 28.008071149547703 30.398157341329675 20.990599354989314 32-33 20.683372690540143 27.985829401787154 30.59162047162524 20.739177436047463 34-35 21.810305396478363 27.414292448978262 30.444660759365572 20.330741395177803 36-37 21.574758727254327 26.762210440138006 31.46235684216539 20.200673990442283 38-39 20.672909763338808 27.512727841131696 31.356580952676776 20.45778144285272 40-41 20.465236982091703 27.966121224934348 31.161168663435628 20.40747312953832 42-43 20.172820865547088 28.30123271002334 29.891477233162888 21.63446919126668 44-45 20.544232681513172 27.456598896405932 29.959047292237017 22.04012112984388 46-47 21.60093376949319 27.978719673781814 29.46253149718717 20.957815059537825 48-49 21.17325278209839 27.6971514017568 29.761677549599376 21.367918266545438 50-51 19.876777972923016 28.22579917985567 30.61454719236322 21.282875654858096 52-53 20.484449311357416 28.91938167941226 29.25982068342278 21.336348325807542 54-55 19.955857143859024 28.043086874062617 29.47191879157178 22.52913719050658 56-57 20.81011057755407 28.0770005299948 28.763288692876532 22.349600199574603 58-59 21.36819357210914 27.62050416834065 29.003455499795116 22.007846759755097 60-61 20.653302127813056 28.15264589014882 29.832614310589772 21.361437671448353 62-63 20.014617436929115 28.234345867192722 29.807047828774607 21.943988867103553 64-65 19.647678692906588 29.233421131463754 29.10267572233082 22.01622445329884 66-67 19.99044193046955 28.425187817569114 28.317384014479373 23.26698623748196 68-69 19.884291832249705 28.25948325567858 28.585848617568427 23.270376294503286 70-71 20.79588940425517 28.00866843969588 27.97921257665162 23.216229579397325 72-73 20.424586949704597 27.332453014307855 29.010703089699362 23.23225694628818 74-75 19.807278261296453 28.978943476906156 28.018089954845244 23.19568830695215 76-77 20.099282209681018 27.93605824556301 28.286905528966088 23.67775401578988 78-79 20.17913894242478 28.003614048485982 28.386115880064906 23.431131129024337 80-81 20.732174657920247 27.7611715996784 27.86386440542118 23.64278933698018 82-83 20.38977160129563 27.411902140317373 28.60388950059261 23.59443675779438 84-85 20.223409339329923 27.567193622429297 28.32967479959885 23.879722238641932 86-87 20.1902893722909 27.712815058596885 28.082306550008028 24.01458901910419 88-89 20.05536159676193 27.59320282617534 27.48635936691984 24.86507621014289 90-91 20.069725268376978 27.035068594995654 27.70032200213998 25.19488413448738 92-93 20.481644045874106 27.3677693029817 27.661470580279186 24.489116070865013 94-95 20.306916081484946 26.948732771411944 27.818214789159253 24.92613635794386 96-97 19.71921296273105 26.790335459657776 28.345003571704087 25.145448005907088 98-99 20.066454868698283 27.150866478112373 27.380898708673595 25.401779944515745 100-101 19.888409819895774 26.493901263028913 27.944433340196262 25.67325557687905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2026.0 1 1372.0 2 753.0 3 787.5 4 711.5 5 709.5 6 1049.0 7 1736.0 8 2373.0 9 2590.0 10 2254.0 11 1904.0 12 1851.5 13 1848.5 14 1979.0 15 2076.0 16 2095.5 17 2195.0 18 2420.5 19 2596.5 20 2794.5 21 3141.5 22 3609.0 23 4242.5 24 4903.5 25 5756.5 26 7288.5 27 9173.0 28 10374.5 29 11530.0 30 13035.0 31 14869.0 32 17379.5 33 19376.5 34 21041.5 35 22867.5 36 25424.5 37 27784.0 38 28574.0 39 28393.5 40 28123.5 41 28200.5 42 28153.5 43 29071.5 44 31030.5 45 33225.0 46 37362.5 47 46038.0 48 56048.5 49 55094.5 50 45459.0 51 37827.0 52 32031.0 53 28026.5 54 25324.5 55 22801.0 56 20361.0 57 17887.0 58 16363.0 59 14791.0 60 12148.0 61 9695.5 62 7635.0 63 5569.0 64 3795.5 65 2688.5 66 1932.5 67 1514.0 68 1173.0 69 843.0 70 612.0 71 393.5 72 274.0 73 240.5 74 179.0 75 133.5 76 89.0 77 45.5 78 22.0 79 11.5 80 9.0 81 9.0 82 6.0 83 3.0 84 2.5 85 3.0 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3807146013066526 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.504701324385872E-4 16-17 0.0023043252184350026 18-19 5.009402648771745E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.504701324385872E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009267394900227727 36-37 0.01863497785343089 38-39 0.011421438039199578 40-41 0.00941767697969088 42-43 0.0037570519865788086 44-45 0.002053855085996415 46-47 0.0018033849535578279 48-49 5.009402648771745E-5 50-51 6.011283178526093E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.007522119017396E-4 60-61 9.016924767789139E-4 62-63 0.0 64-65 0.0 66-67 0.001352538715168371 68-69 8.515984502911965E-4 70-71 0.0019536670330209806 72-73 0.0 74-75 0.0 76-77 0.09673156514778239 78-79 0.03506581854140221 80-81 5.009402648771745E-5 82-83 0.0 84-85 0.0 86-87 0.14857888256256993 88-89 0.3215535560246583 90-91 0.2793242916955125 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.003761059508698E-4 100-101 6.512223443403268E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 998123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.711670385910374 #Duplication Level Percentage of deduplicated Percentage of total 1 80.87813776726904 45.05856152716341 2 11.208794835170586 12.489213665606366 3 3.2344403136748454 5.4058821791506055 4 1.4374727863559276 3.2033604024871036 5 0.7612869358527236 2.120628341966331 6 0.4826855993754012 1.6134732607456765 7 0.3154122410662801 1.2300499966976128 8 0.239181503751076 1.0660160879509062 9 0.16921891076954831 0.8484721361870269 >10 1.126540494214805 11.804449641995113 >50 0.09729268536941656 3.716315757688831 >100 0.04253628547933681 4.193688518912002 >500 0.0025126918747428485 1.0529966570932285 >1k 0.0039485158031673335 4.336674391300961 >5k 5.384339731591819E-4 1.8602174350548308 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7692 0.7706465034870452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5562 0.5572459506493688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5350 0.5360060834185766 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4705 0.4713847892494212 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3318 0.33242395977249295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2902 0.290745729734712 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2449 0.24536054173684005 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2379 0.23834737802855963 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2377 0.23814700192260874 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2293 0.2297312054726722 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2185 0.21891089575132522 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2028 0.20318137143418197 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2007 0.2010774223216978 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1875 0.18785259932894044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.18614940242835804 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1650 0.16531028740946757 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1625 0.1628055860850817 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1377 0.13795894894717384 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1340 0.13425199098708274 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1264 0.1266376989609497 RNA PCR Primer, Index 23 (95% over 23bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1193 0.11952434719969382 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1190 0.11922378304076751 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1188 0.11902340693481667 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 0.11671908171638165 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1003 0.1004886171343612 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.001880529754349E-4 0.0 8 0.0 0.0 0.0 1.001880529754349E-4 0.0 9 0.0 0.0 0.0 1.001880529754349E-4 0.0 10-11 0.0 0.0 0.0 2.5047013243858726E-4 0.0 12-13 0.0 0.0 0.0 3.005641589263047E-4 0.0 14-15 0.0 0.0 0.0 3.005641589263047E-4 0.0 16-17 0.0 0.0 0.0 3.005641589263047E-4 0.0 18-19 0.0 0.0 0.0 4.007522119017395E-4 0.0 20-21 0.0 0.0 0.0 0.0011020685827297838 0.0 22-23 0.0 0.0 0.0 0.003356299774677069 0.0 24-25 0.0 0.0 0.0 0.00686288162881729 0.0 26-27 0.0 0.0 0.0 0.012924258833831102 0.0 28-29 5.009402648771745E-5 0.0 0.0 0.03551666477979167 0.0 30-31 1.001880529754349E-4 0.0 0.0 0.0841579644993653 0.0 32-33 1.001880529754349E-4 0.0 0.0 0.19080814689171577 0.0 34-35 1.001880529754349E-4 0.0 0.0 0.36573648738682507 0.0 36-37 1.001880529754349E-4 0.0 0.0 0.6151045512426825 0.0 38-39 1.001880529754349E-4 0.0 0.0 0.9328008672277865 0.0 40-41 1.001880529754349E-4 0.0 0.0 1.2505472772393782 0.0 42-43 1.5028207946315235E-4 0.0 0.0 1.5765592016214434 0.0 44-45 2.003761059508698E-4 0.0 0.0 2.0008055119459227 0.0 46-47 3.005641589263047E-4 0.0 0.0 2.497638066651104 0.0 48-49 3.005641589263047E-4 0.0 0.0 2.9593046147619084 1.001880529754349E-4 50-51 3.005641589263047E-4 0.0 0.0 3.410852169522193 1.001880529754349E-4 52-53 3.005641589263047E-4 0.0 0.0 3.8480227386805033 1.001880529754349E-4 54-55 3.005641589263047E-4 0.0 0.0 4.339395044498524 1.001880529754349E-4 56-57 3.005641589263047E-4 0.0 0.0 4.970078837978886 1.001880529754349E-4 58-59 3.005641589263047E-4 0.0 0.0 5.593398809565555 1.001880529754349E-4 60-61 3.005641589263047E-4 0.0 0.0 6.238860340859794 1.001880529754349E-4 62-63 3.005641589263047E-4 0.0 0.0 6.861979936340511 1.001880529754349E-4 64-65 3.005641589263047E-4 0.0 0.0 7.5168591446144415 1.001880529754349E-4 66-67 3.005641589263047E-4 0.0 0.0 8.273980260949802 1.001880529754349E-4 68-69 3.005641589263047E-4 0.0 0.0 9.10078216812958 1.001880529754349E-4 70-71 3.005641589263047E-4 0.0 0.0 9.975824622817028 1.001880529754349E-4 72-73 3.005641589263047E-4 0.0 0.0 10.827573355187688 1.001880529754349E-4 74-75 3.005641589263047E-4 0.0 0.0 11.738683508946291 1.001880529754349E-4 76-77 3.005641589263047E-4 0.0 0.0 12.670732965776763 1.001880529754349E-4 78-79 3.005641589263047E-4 0.0 0.0 13.732075104972033 1.001880529754349E-4 80-81 3.005641589263047E-4 0.0 0.0 14.84832029719784 1.001880529754349E-4 82-83 3.005641589263047E-4 0.0 0.0 15.938616783703011 1.001880529754349E-4 84-85 3.005641589263047E-4 0.0 0.0 17.054010377478527 1.001880529754349E-4 86-87 3.005641589263047E-4 0.0 0.0 18.171257450234087 1.001880529754349E-4 88-89 3.005641589263047E-4 0.0 0.0 19.343858422258577 1.001880529754349E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4780 0.0 21.720076 1 TACATGG 4605 0.0 21.228676 2 ACATGGG 4305 0.0 20.503363 3 GCTACCG 60 0.004790687 19.77307 62-63 ATGGGAG 875 0.0 19.524492 5 GAGTACT 5285 0.0 18.766644 12-13 CGGAGAT 130 0.0074488963 18.317213 1 CATGGGG 1430 0.0 18.252062 4 CATGGGA 2385 0.0 17.907686 4 CCCTATA 240 3.1171148E-6 17.795763 2 AGTACTT 5495 0.0 17.185837 12-13 GTACTTT 5830 0.0 16.930902 14-15 GTGTTAA 320 1.5550722E-7 16.37101 1 GTATAGT 175 0.0021317806 16.328487 1 TCTATAC 240 6.1955885E-5 15.818456 3 TAAGGCG 215 4.8209756E-4 15.450584 5 GTGTAGC 435 1.3624231E-9 15.327506 1 GTACTGC 565 1.8189894E-12 15.118523 6 ACTAGTA 220 5.7406933E-4 15.099434 8 TAAGGTG 315 2.45929E-6 15.065195 5 >>END_MODULE