FastQCFastQC Report
Thu 26 May 2016
SRR938810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992973
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70610.7110968777600196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.6008219760255314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52530.529017405307093No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49290.49638811931442245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.34210396455895575No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29630.298396834556428No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24900.25076210531404175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24020.24189983010615596No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21860.22014697277770895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.21299672800771016No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20630.2077599290212322No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18380.18510070263743325No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18350.1847985796189826No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18200.18328796452672932No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16100.16213935323518364No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16060.1617365225439161No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.1317256360444846No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC12460.12548176032983777No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11920.12004354599772601No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11780.11863363857828964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.1099727787160376No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC10870.10946924035195318No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT9960.10030484212561672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT50650.021.36904312-13
CCGTTGT1200.004799406519.765669
GTACTTT55400.019.2374414-15
GTACATG45550.018.9973751
TACCTGG4500.018.9798282
GTAAGGT3054.620233E-918.668684
TACATGG44500.018.659942
GGTAAGG2803.529567E-818.6409023
AGTACTT53300.018.39172712-13
CCGTATC1555.5715645E-918.39172694-95
ACATGGG43350.018.388763
AGTATCG1600.001199371317.7935875
CATGGGA24200.016.8622424
GTAAGCG1551.11444024E-716.85908194-95
CATGGGG15250.016.8018134
ACTTTTT65000.016.6526816-17
CCGATTA1359.53203E-615.82491516-17
CAATACA2751.13889055E-515.5289494
CCAATAC3705.016227E-815.3890493
TACTTTT63900.015.3412214-15
Produced by FastQC (version 0.11.5)