##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938810_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992973 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54832910864646 33.0 31.0 34.0 30.0 34.0 2 31.588130795097147 33.0 31.0 34.0 30.0 34.0 3 31.586038089656014 33.0 31.0 34.0 28.0 34.0 4 35.13342356740818 37.0 35.0 37.0 33.0 37.0 5 35.169348008455415 37.0 35.0 37.0 33.0 37.0 6 35.259113792620745 37.0 35.0 37.0 33.0 37.0 7 35.19117136115484 37.0 35.0 37.0 32.0 37.0 8 35.17543880850738 37.0 35.0 37.0 32.0 37.0 9 36.73042771555722 39.0 37.0 39.0 33.0 39.0 10-11 36.62181801519276 39.0 37.0 39.0 32.0 39.0 12-13 36.53146913360182 39.0 37.0 39.0 32.0 39.0 14-15 37.62523603360816 40.0 37.0 41.0 32.0 41.0 16-17 37.53861585360327 40.0 37.0 41.0 32.0 41.0 18-19 37.538447168251295 40.0 37.0 41.0 32.0 41.0 20-21 37.444766373305214 40.0 37.0 41.0 32.0 41.0 22-23 37.426786528938855 40.0 37.0 41.0 32.0 41.0 24-25 37.3350750725347 40.0 37.0 41.0 32.0 41.0 26-27 37.163742115848066 40.0 37.0 41.0 31.0 41.0 28-29 36.99288802414567 40.0 36.0 41.0 31.0 41.0 30-31 36.76481938582418 39.0 36.0 41.0 30.0 41.0 32-33 36.54464471843645 39.0 35.5 41.0 30.0 41.0 34-35 36.21192469483057 39.0 35.0 41.0 30.0 41.0 36-37 35.9453147265837 39.0 35.0 41.0 29.5 41.0 38-39 35.747244386302555 39.0 35.0 40.5 28.5 41.0 40-41 35.63547750039528 39.0 35.0 40.5 27.0 41.0 42-43 35.57126477759214 39.0 35.0 41.0 27.0 41.0 44-45 35.33995385574431 39.0 35.0 41.0 26.0 41.0 46-47 34.95496151456283 39.0 35.0 41.0 24.0 41.0 48-49 34.5731651313782 39.0 34.0 41.0 23.0 41.0 50-51 33.382566293343324 38.0 32.5 39.5 19.5 40.5 52-53 33.4485267978082 37.5 33.0 39.5 19.0 40.5 54-55 33.95725513181124 38.0 34.0 40.0 19.0 41.0 56-57 33.83464505077177 38.0 34.0 40.0 17.5 41.0 58-59 33.63065309932898 38.0 34.0 40.0 14.5 41.0 60-61 33.28182639407114 38.0 33.0 40.0 12.0 41.0 62-63 32.89520560982021 37.0 33.0 40.0 8.0 41.0 64-65 32.49050628768355 36.5 32.0 39.5 6.5 41.0 66-67 32.06448312290465 36.0 32.0 39.0 2.0 41.0 68-69 31.616358148710994 35.5 31.0 39.0 2.0 40.5 70-71 31.15040590227529 35.0 31.0 38.5 2.0 40.0 72-73 30.684899790830162 35.0 30.5 37.0 2.0 39.5 74-75 30.202759793065873 35.0 30.0 37.0 2.0 39.0 76-77 29.723462772905204 35.0 29.5 36.0 2.0 39.0 78-79 29.257145964693905 34.0 29.0 36.0 2.0 38.5 80-81 28.754104592974834 34.0 29.0 35.5 2.0 37.0 82-83 28.309417778731145 34.0 28.5 35.0 2.0 37.0 84-85 27.91568048678061 34.0 27.5 35.0 2.0 36.0 86-87 27.47607941001417 34.0 26.5 35.0 2.0 36.0 88-89 26.973748530926823 34.0 25.5 35.0 2.0 35.5 90-91 26.47844503324864 33.5 25.0 35.0 2.0 35.0 92-93 25.97659553683736 33.0 23.5 35.0 2.0 35.0 94-95 25.422486814847936 33.0 19.5 35.0 2.0 35.0 96-97 24.788384981263334 32.5 8.5 35.0 2.0 35.0 98-99 24.026877367259733 32.0 2.0 35.0 2.0 35.0 100-101 21.70467374238776 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21854014960648982 1101 2 0.2033077433986712 1101 3 0.2782823941489454 1101 4 0.05021365702670266 1101 5 0.0022779168119271276 1101 6 0.07352123990970938 1101 7 0.13823863276729043 1101 8 0.24251688167792906 1101 9 0.4183085390228598 1101 10-11 0.2870304380062336 1101 12-13 0.24220813572888744 1101 14-15 0.30159523295235147 1101 16-17 0.5017199021082561 1101 18-19 0.4556769351395573 1101 20-21 0.620142502656293 1101 22-23 0.4335236932810034 1101 24-25 0.5120689086332249 1101 26-27 0.4333865948086313 1101 28-29 0.5149521130157382 1101 30-31 0.44259754898003933 1101 32-33 0.5546385417334534 1101 34-35 0.6178456708937077 1101 36-37 0.7788792982511268 1101 38-39 0.5865107435458086 1101 40-41 0.6520613308113568 1101 42-43 0.7822673434902327 1101 44-45 0.5796770046977571 1101 46-47 0.46364017041192795 1101 48-49 0.25935766853822173 1101 50-51 0.418255323345889 1101 52-53 0.3030097101087179 1101 54-55 0.329862033628352 1101 56-57 0.29983096959353617 1101 58-59 0.3872212842905256 1101 60-61 0.4683463188869723 1101 62-63 0.16914414739382977 1101 64-65 0.21218960877363457 1101 66-67 0.21378068824304108 1101 68-69 0.32917789782364437 1101 70-71 0.3866958318236584 1101 72-73 0.4258780867515135 1101 74-75 0.21238246512558945 1101 76-77 0.05404373791247252 1101 78-79 0.05318315343090063 1101 80-81 0.017000223051446994 1101 82-83 0.06916382207854 1101 84-85 -0.10703434659063049 1101 86-87 -0.16315042236747956 1101 88-89 -0.19642503089173502 1101 90-91 -0.04014212236575432 1101 92-93 -0.3699269782215744 1101 94-95 -0.327922674313573 1101 96-97 -0.25524973909945814 1101 98-99 -0.4398360718067522 1101 100-101 0.12277935403430362 1102 1 -0.21146861439348186 1102 2 -0.23502797070630876 1102 3 -0.3060003725147311 1102 4 -0.005396338073744289 1102 5 -0.1666579587714736 1102 6 0.009740712551540298 1102 7 0.08420891797683794 1102 8 0.0080672309727845 1102 9 -0.11249197531582666 1102 10-11 0.0499050069945568 1102 12-13 -0.031140678370796593 1102 14-15 0.06010965444784233 1102 16-17 -0.11362100828961985 1102 18-19 0.11788051756830953 1102 20-21 0.06230757869146686 1102 22-23 0.06875669836688303 1102 24-25 0.013740624375991217 1102 26-27 0.06273955398126674 1102 28-29 0.11818386675884085 1102 30-31 0.14841985830553028 1102 32-33 0.2329065821168541 1102 34-35 0.018629025274442768 1102 36-37 0.172151720634524 1102 38-39 0.14002462447961506 1102 40-41 0.2617274937205494 1102 42-43 0.3155630675323522 1102 44-45 0.3660061998589228 1102 46-47 0.4251113492734433 1102 48-49 0.3149118822740107 1102 50-51 0.28241447390497143 1102 52-53 0.22660419223912953 1102 54-55 0.3404788950122395 1102 56-57 0.18393454581157442 1102 58-59 0.11025760773502924 1102 60-61 0.10456096097763634 1102 62-63 0.08476994529736714 1102 64-65 -0.11390077313894409 1102 66-67 -0.1448716339869236 1102 68-69 0.0839440416406525 1102 70-71 0.09368379915072822 1102 72-73 0.012357604064440864 1102 74-75 -0.09416028640236007 1102 76-77 -0.04660309589126399 1102 78-79 -0.20328345940962578 1102 80-81 -0.19885985658363836 1102 82-83 -0.32314939085905436 1102 84-85 -0.12106479746507759 1102 86-87 -0.13568126313641216 1102 88-89 -0.41127989583182867 1102 90-91 -0.40249556689377286 1102 92-93 -0.36802983675773504 1102 94-95 -0.39155886020238384 1102 96-97 -0.3481964443185248 1102 98-99 -0.6391566892892051 1102 100-101 -0.346363775196739 1103 1 0.06825703065152666 1103 2 0.23186489990106907 1103 3 0.3108683564440007 1103 4 0.09566457163649034 1103 5 0.0368462180644471 1103 6 0.05804054521353663 1103 7 0.12627822104381892 1103 8 0.249126553286942 1103 9 0.2807688669300745 1103 10-11 0.26711535681697285 1103 12-13 0.42894457802674424 1103 14-15 0.29748654857514367 1103 16-17 0.3339850000045246 1103 18-19 0.3102613471150377 1103 20-21 0.37172910510507506 1103 22-23 0.1343806442366784 1103 24-25 0.08820107506419816 1103 26-27 0.3351201851034986 1103 28-29 0.15395153811493856 1103 30-31 0.053948295844797656 1103 32-33 0.12575073289303162 1103 34-35 0.17681800477718923 1103 36-37 0.16033554110003223 1103 38-39 0.2645674213392155 1103 40-41 0.47278172313896505 1103 42-43 0.44615119777819245 1103 44-45 0.43784413994794846 1103 46-47 0.27353982715903413 1103 48-49 0.4061458181564319 1103 50-51 0.33548310894276767 1103 52-53 0.3791992541145035 1103 54-55 0.3166381902083799 1103 56-57 0.43250423580635555 1103 58-59 0.5212327980572056 1103 60-61 0.22948572501782394 1103 62-63 0.4129425878488391 1103 64-65 0.2392932000472925 1103 66-67 0.3489110647186848 1103 68-69 0.3452509316010328 1103 70-71 0.3236148135629655 1103 72-73 0.37666500331439323 1103 74-75 0.30635793227467545 1103 76-77 0.20484044241458577 1103 78-79 0.11971526405936572 1103 80-81 -0.26892685792631355 1103 82-83 -0.15561957757651967 1103 84-85 -0.045858601329062054 1103 86-87 -0.10852190841291787 1103 88-89 -0.056086795713433446 1103 90-91 -0.15390272737289834 1103 92-93 -0.03935451878245644 1103 94-95 0.16367094845024965 1103 96-97 -0.01762782581610267 1103 98-99 0.10927575708656789 1103 100-101 0.3916861087249224 1104 1 0.28704430869963815 1104 2 0.19330062503687628 1104 3 0.324566295906596 1104 4 0.3772956659268871 1104 5 0.3547577033506144 1104 6 0.35268301923154155 1104 7 0.5190933734171708 1104 8 0.31796703971451734 1104 9 0.5407186193394722 1104 10-11 0.41769882531675506 1104 12-13 0.5093755873553718 1104 14-15 0.6895588218602455 1104 16-17 0.7164980017295051 1104 18-19 0.6159536782428034 1104 20-21 0.6977511136355758 1104 22-23 0.6378531346462992 1104 24-25 0.6094136253761491 1104 26-27 0.4830153431409627 1104 28-29 0.7221070739319089 1104 30-31 0.541135374790592 1104 32-33 0.6424909901812867 1104 34-35 1.0143608074856658 1104 36-37 0.7916738820284266 1104 38-39 0.9324194762983282 1104 40-41 0.8591485777513626 1104 42-43 0.9326265073737261 1104 44-45 0.8589557445238682 1104 46-47 1.0318091173420854 1104 48-49 0.976133610266146 1104 50-51 0.9604316169626443 1104 52-53 0.7525622382463908 1104 54-55 0.6763740363503388 1104 56-57 0.4767910933366224 1104 58-59 0.9018749245043409 1104 60-61 0.8111659512392535 1104 62-63 0.7379717666375782 1104 64-65 0.6266920397613447 1104 66-67 0.5468998737950059 1104 68-69 0.6261568471713481 1104 70-71 0.7304170114123139 1104 72-73 0.4349780616805532 1104 74-75 0.4588294400456725 1104 76-77 0.7648462996273295 1104 78-79 0.42219578292688453 1104 80-81 0.3563429385945476 1104 82-83 0.3583299216639766 1104 84-85 0.30337502636675495 1104 86-87 0.2955227866258312 1104 88-89 0.595131204166421 1104 90-91 0.4714845352276136 1104 92-93 0.5569934125272411 1104 94-95 0.8212433805022918 1104 96-97 0.626654847990789 1104 98-99 0.7123659195437675 1104 100-101 1.139581374037352 1105 1 0.16561561789416857 1105 2 0.16258569468732986 1105 3 0.06147497735291907 1105 4 0.09851419369933012 1105 5 0.16165840445115265 1105 6 0.2296405019547194 1105 7 0.10592872760246053 1105 8 0.04762551434036055 1105 9 0.12615114572475505 1105 10-11 0.26901317991150364 1105 12-13 0.1339572459302758 1105 14-15 0.20257754433640685 1105 16-17 0.15088838269700489 1105 18-19 0.3103957434393081 1105 20-21 0.21153131151473303 1105 22-23 0.1664121181758631 1105 24-25 0.26583049189964214 1105 26-27 0.21807778621310803 1105 28-29 0.1829380953326023 1105 30-31 0.21027274537110685 1105 32-33 0.26909123753023323 1105 34-35 0.363122809340787 1105 36-37 0.37267595858961045 1105 38-39 0.25779300661945825 1105 40-41 0.3603612978375992 1105 42-43 0.20239196459322528 1105 44-45 0.4103490669381955 1105 46-47 0.3733621720178988 1105 48-49 0.3810604292418276 1105 50-51 0.1746548075968093 1105 52-53 0.14680281015724717 1105 54-55 0.2254009355472988 1105 56-57 0.21311326114950901 1105 58-59 0.11435737609999563 1105 60-61 0.06896559024350779 1105 62-63 -0.16363286781244568 1105 64-65 -0.05978587014013925 1105 66-67 0.03020173141894844 1105 68-69 -0.0844072922465422 1105 70-71 -0.311997377443614 1105 72-73 -0.16517057748247055 1105 74-75 -0.125006494908245 1105 76-77 -0.019683841846237726 1105 78-79 -0.26242230947799783 1105 80-81 -0.09273065189254837 1105 82-83 -0.09008247001640868 1105 84-85 -0.23099268791179384 1105 86-87 -0.3423951369801159 1105 88-89 -0.4282092181651791 1105 90-91 -0.31310462320104904 1105 92-93 -0.5440289909356473 1105 94-95 -0.1775256198236086 1105 96-97 -0.03627164090775992 1105 98-99 0.10554940900219023 1105 100-101 0.22960886653749668 1106 1 0.33319723027427983 1106 2 0.26752640153993923 1106 3 0.22337118163817138 1106 4 0.35103921061053 1106 5 0.4665559078815633 1106 6 0.25749352512939794 1106 7 0.4152114798024371 1106 8 0.23702586909521983 1106 9 0.4176728290761176 1106 10-11 0.5021209967898486 1106 12-13 0.5137280041097654 1106 14-15 0.4330698300915543 1106 16-17 0.6372483726770639 1106 18-19 0.6054360717041831 1106 20-21 0.6254976797802954 1106 22-23 0.5006367333763677 1106 24-25 0.4389829117476651 1106 26-27 0.3829688192718308 1106 28-29 0.22067871861803212 1106 30-31 0.13073889609831468 1106 32-33 0.23276283878495008 1106 34-35 0.4397485409339481 1106 36-37 0.4392939115384493 1106 38-39 0.3432729043510818 1106 40-41 0.4825321582449078 1106 42-43 0.4009907944498323 1106 44-45 0.19293443679903532 1106 46-47 0.00857671351904088 1106 48-49 -0.08152956521069399 1106 50-51 -0.13797620982349912 1106 52-53 -0.014657810329090637 1106 54-55 -0.23459026150189288 1106 56-57 -0.13478381734583422 1106 58-59 -0.018447509740539658 1106 60-61 -0.15260863698823357 1106 62-63 -0.3199841258332228 1106 64-65 -0.09238772017963726 1106 66-67 -0.03118769469558913 1106 68-69 -0.1307419483888843 1106 70-71 -0.043451598549804515 1106 72-73 -0.18377644564306905 1106 74-75 -0.23955134279873036 1106 76-77 -0.14913894121917792 1106 78-79 -0.16258386593706575 1106 80-81 -0.31518973794747396 1106 82-83 -0.2541941173953539 1106 84-85 -0.06311493939830726 1106 86-87 -0.21114598000463758 1106 88-89 0.010189395268650259 1106 90-91 0.07222477246826386 1106 92-93 -0.06814025293194703 1106 94-95 0.06919228921188747 1106 96-97 0.3514055463669088 1106 98-99 0.09792238232052952 1106 100-101 0.23756955345162467 1107 1 0.11670912052671056 1107 2 0.28115993161222974 1107 3 0.2860962093108306 1107 4 0.2337870557395405 1107 5 0.2715615622950054 1107 6 0.07831355493994607 1107 7 0.27827976833336976 1107 8 0.166086956740628 1107 9 0.2259652361779061 1107 10-11 0.24985573486862478 1107 12-13 0.25945172906951086 1107 14-15 0.4097503697183953 1107 16-17 0.4129045243991598 1107 18-19 0.37337235066056707 1107 20-21 0.388494339080907 1107 22-23 0.532551201881482 1107 24-25 0.5008273094827018 1107 26-27 0.4922243747829853 1107 28-29 0.5106162603268274 1107 30-31 0.33023928970942507 1107 32-33 0.3945529901154359 1107 34-35 0.49253488802302314 1107 36-37 0.36443037671085676 1107 38-39 0.33104398652189815 1107 40-41 0.14143266454468773 1107 42-43 0.3327512151541754 1107 44-45 0.42311851492984687 1107 46-47 0.36790070141899633 1107 48-49 0.0510014941027066 1107 50-51 0.1968290026266004 1107 52-53 0.033986129458398295 1107 54-55 0.24835059293392447 1107 56-57 0.610423917412227 1107 58-59 0.45896680491657804 1107 60-61 0.4849089955907715 1107 62-63 0.3209621192766079 1107 64-65 0.27636195514114803 1107 66-67 0.10663613536031136 1107 68-69 0.22305849677129785 1107 70-71 0.2476517913010241 1107 72-73 0.20133206714629281 1107 74-75 0.26020040247694 1107 76-77 0.07107798445825253 1107 78-79 -0.022247518248580178 1107 80-81 0.029421286360506116 1107 82-83 0.07329032797262514 1107 84-85 0.17184741779278667 1107 86-87 0.02598523435557354 1107 88-89 0.21593312046815427 1107 90-91 0.23123067463692237 1107 92-93 0.4035452218189981 1107 94-95 0.42925554671347754 1107 96-97 0.5207767076595502 1107 98-99 0.42071845979598166 1107 100-101 0.8972572819758433 1108 1 0.11961722779395245 1108 2 0.24326163326548667 1108 3 0.08730136698815016 1108 4 0.1307242527216772 1108 5 0.3007787499435395 1108 6 0.30711418755050346 1108 7 0.08657154698881442 1108 8 0.21699179724844697 1108 9 0.3198391980311399 1108 10-11 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2316 76-77 -1.4303293554653322 2316 78-79 -1.1600733480352332 2316 80-81 -1.193827357290143 2316 82-83 -1.172829289122138 2316 84-85 -1.4047914193501079 2316 86-87 -1.3361515427680146 2316 88-89 -1.4124492544689566 2316 90-91 -1.631232442179094 2316 92-93 -1.5887840957231205 2316 94-95 -1.659401769414501 2316 96-97 -2.001225726290656 2316 98-99 -2.215915890176145 2316 100-101 -1.9734697076764682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7031.0 3 1319.0 4 1134.0 5 1149.0 6 1311.0 7 1653.0 8 2102.0 9 2480.0 10 3024.0 11 3954.0 12 5146.0 13 6537.0 14 7424.0 15 8470.0 16 9935.0 17 12150.0 18 11071.0 19 7851.0 20 7676.0 21 8338.0 22 8868.0 23 9531.0 24 10700.0 25 12326.0 26 13993.0 27 16481.0 28 19091.0 29 22039.0 30 26709.0 31 31525.0 32 38598.0 33 47430.0 34 61878.0 35 85813.0 36 120925.0 37 176970.0 38 155345.0 39 24992.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09405881604485 20.08797565118341 15.140052147183509 20.67791338558823 2 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19.937481516471912 20-21 21.293759336325664 27.337619193442364 30.330893759739926 21.03772771049205 22-23 20.529999878861783 27.129710762322784 30.822154968080078 21.518134390735348 24-25 21.58338010085635 27.42516523403276 30.700422724574956 20.291031940535937 26-27 21.09169030409164 26.45141536555568 32.313311499661985 20.14358283069069 28-29 19.856198322061207 28.72507967936317 31.46766608917017 19.95105590940545 30-31 20.3331305026246 27.766884394070612 30.74708188890095 21.152903214403842 32-33 20.431983267912663 27.66444416306521 30.94714773099316 20.956424838028976 34-35 21.709053608153653 27.138995143987586 30.641488840267005 20.51046240759175 36-37 21.441898860426946 26.528298243299325 31.72823535775254 20.30156753852119 38-39 20.690406311928164 27.45290490310588 31.518803923778542 20.337884861187412 40-41 20.522741889119427 27.885697195476023 31.182438798651518 20.409122116753032 42-43 20.029504205031483 28.195467632552873 29.893905142818443 21.8811230195972 44-45 20.620250170331747 27.335971032483325 30.03428874112203 22.0094900560629 46-47 21.474452938452924 27.940019854548247 29.704941598917905 20.880585608080924 48-49 21.118186699130337 27.681260825295777 29.925136728604464 21.275415746969422 50-51 19.968627696799782 28.131341062331096 30.79798683434959 21.102044406519536 52-53 20.466553834767996 28.753986019359367 29.373600975449964 21.405859170422676 54-55 20.002759956568596 27.879857978498624 29.720839997331034 22.39654206760175 56-57 20.80287212204093 27.728116696129774 29.140677984946024 22.32833319688328 58-59 21.60136415308829 27.130781861816345 29.28861942655046 21.979234558544903 60-61 20.80191159335306 27.5153680529355 30.420032334656117 21.262688019055325 62-63 20.303922762820257 27.398082110497242 30.468443980351058 21.82955114633144 64-65 19.979856986627446 28.41504719501966 29.83997326399229 21.7651225543606 66-67 20.192731146673975 27.777949125813223 29.05842456773525 22.970895159777548 68-69 20.064944685270593 27.502081981793847 29.588917940842897 22.844055392092667 70-71 20.99434327764257 27.311041859340925 29.03445357032878 22.660161292687725 72-73 20.679483925507142 26.613288948527043 30.075425183051934 22.631801942913885 74-75 20.058565893961557 28.254302575958963 29.17788834157506 22.50924318850442 76-77 20.30637803136571 27.304090685066534 29.258621779075206 23.13090950449255 78-79 20.475014680599678 27.319004124717882 29.507398921165436 22.698582273517008 80-81 21.0447789859504 27.02174609640739 29.11836980594998 22.81510511169223 82-83 20.834565422547207 26.58840941452637 29.801445348060778 22.775579814865644 84-85 20.790462166969704 26.809232724278438 29.552128052667147 22.84817705608471 86-87 20.64497182885734 26.921675276972852 29.504059505378727 22.929293388791088 88-89 20.770129770344102 26.740920322060152 28.93602673859505 23.5529231690007 90-91 20.815203487402055 26.267808370857015 29.210531371753945 23.706456769986985 92-93 21.498685722635148 26.4750418753694 29.00986049295892 23.016411909036535 94-95 21.352213660174343 25.984568088505956 29.201909269259552 23.46130898206015 96-97 20.78529168316301 25.662546246645746 29.818310157550826 23.73385191264042 98-99 21.343098688401913 26.068492319516217 28.9228614479153 23.665547544166575 100-101 21.547932090434205 25.066446677362837 29.538821136362024 23.84680009584093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2842.0 1 1921.5 2 1037.0 3 1009.5 4 870.5 5 898.0 6 1250.5 7 1988.0 8 2666.5 9 2799.0 10 2438.5 11 2115.5 12 2094.5 13 2130.5 14 2232.5 15 2268.0 16 2335.5 17 2455.0 18 2581.5 19 2798.0 20 3081.0 21 3547.5 22 4057.5 23 4584.5 24 5198.5 25 6120.0 26 7644.5 27 9449.5 28 10651.0 29 11789.0 30 13272.5 31 14974.5 32 17219.0 33 19062.5 34 20837.0 35 22889.0 36 25492.0 37 27666.5 38 28406.0 39 28211.0 40 27706.0 41 28025.0 42 28154.5 43 28564.5 44 30206.5 45 32808.5 46 37412.5 47 46149.0 48 56708.5 49 56006.0 50 43563.0 51 33332.0 52 28731.5 53 26614.5 54 24589.0 55 22234.5 56 19929.5 57 17505.0 58 15758.0 59 14228.0 60 11941.0 61 9657.5 62 7753.5 63 5740.0 64 3978.5 65 2901.5 66 2207.0 67 1772.5 68 1352.5 69 1017.0 70 782.0 71 548.5 72 397.5 73 324.5 74 252.5 75 184.0 76 140.5 77 96.0 78 58.0 79 42.0 80 36.5 81 27.5 82 16.0 83 10.5 84 8.0 85 7.5 86 7.0 87 5.0 88 5.0 89 4.5 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2718100089327706 2 0.15649972355743813 3 0.18832334816757354 4 0.15498910846518485 5 0.22971420169531298 6 0.18600707169278521 7 0.23424604697207274 8 0.2376701078478468 9 0.14119215728927173 10-11 0.19794093092158596 12-13 0.20378197594496528 14-15 0.16415350669152132 16-17 0.2779028231381921 18-19 0.22417527969038434 20-21 0.18978360942341835 22-23 0.23857647690319878 24-25 0.22729721754770776 26-27 0.3393848573928999 28-29 0.3089711402022009 30-31 0.284045991180022 32-33 0.18364044138158842 34-35 0.3502612860571234 36-37 0.390191878329018 38-39 0.3697482207471905 40-41 0.32916302860198615 42-43 0.36481354477916317 44-45 0.37533749658852755 46-47 0.38057429557500555 48-49 0.39980946108303045 50-51 0.3911486012207784 52-53 0.3918535549304966 54-55 0.38540826387021604 56-57 0.3625476221407833 58-59 0.3371692885909285 60-61 0.3034825720336807 62-63 0.37347440464141524 64-65 0.4073121827078884 66-67 0.4111390742749299 68-69 0.41607375024295723 70-71 0.4178864883536612 72-73 0.40746324421711366 74-75 0.4029817527767623 76-77 0.3862642790891595 78-79 0.3597278073019105 80-81 0.37181272803993665 82-83 0.3826388028677517 84-85 0.38445154097845563 86-87 0.3778048345725412 88-89 0.3868181712896524 90-91 0.4070604135258461 92-93 0.4054994445971844 94-95 0.4142106582958449 96-97 0.41466384282352087 98-99 0.41320358156767606 100-101 0.38611321757993416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 992973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.3370823936744 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56708389756288 46.76818610046315 2 10.831486513149562 12.420916693008587 3 3.173227287508388 5.45830783313173 4 1.3488943907849462 3.0936667529920676 5 0.7288027678514503 2.089371217451828 6 0.4668121386494288 1.6059387633665791 7 0.2995850717555849 1.2024133760215379 8 0.22789133624547148 1.0453299458488923 9 0.16870431445124281 0.8705711860073337 >10 1.0559639428506131 11.490041842137218 >50 0.0879377365748024 3.495080727858867 >100 0.037455880704260563 3.750454552609555 >500 0.0022860395670916177 0.9991935804800773 >1k 0.0033411347519031334 3.8674135384938775 >5k 5.27547592405758E-4 1.8431138901287398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7061 0.7110968777600196 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5966 0.6008219760255314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5253 0.529017405307093 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4929 0.49638811931442245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3397 0.34210396455895575 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2963 0.298396834556428 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2490 0.25076210531404175 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2402 0.24189983010615596 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2186 0.22014697277770895 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2115 0.21299672800771016 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2063 0.2077599290212322 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1838 0.18510070263743325 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1835 0.1847985796189826 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1820 0.18328796452672932 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1610 0.16213935323518364 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1606 0.1617365225439161 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.1317256360444846 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1246 0.12548176032983777 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1192 0.12004354599772601 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1178 0.11863363857828964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.1099727787160376 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1087 0.10946924035195318 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 996 0.10030484212561672 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0070767281688425E-4 0.0 4 0.0 0.0 0.0 1.0070767281688425E-4 0.0 5 0.0 0.0 0.0 1.0070767281688425E-4 0.0 6 0.0 0.0 0.0 2.014153456337685E-4 0.0 7 0.0 0.0 0.0 2.014153456337685E-4 0.0 8 0.0 0.0 0.0 3.0212301845065274E-4 0.0 9 0.0 0.0 0.0 3.0212301845065274E-4 0.0 10-11 0.0 0.0 0.0 4.02830691267537E-4 0.0 12-13 0.0 0.0 0.0 4.02830691267537E-4 0.0 14-15 0.0 0.0 0.0 5.035383640844212E-4 0.0 16-17 5.0353836408442124E-5 0.0 0.0 5.538922004928634E-4 0.0 18-19 1.0070767281688425E-4 0.0 0.0 6.545998733097476E-4 1.0070767281688425E-4 20-21 1.0070767281688425E-4 0.0 0.0 0.001158138237394169 1.0070767281688425E-4 22-23 1.0070767281688425E-4 0.0 0.0 0.0035247685485909484 1.0070767281688425E-4 24-25 1.0070767281688425E-4 0.0 0.0 0.006294229551055266 1.0070767281688425E-4 26-27 1.0070767281688425E-4 0.0 0.0 0.012135274574434552 1.0070767281688425E-4 28-29 3.0212301845065274E-4 0.0 0.0 0.03182362461013542 1.0070767281688425E-4 30-31 3.0212301845065274E-4 0.0 0.0 0.08132144579963403 1.0070767281688425E-4 32-33 3.0212301845065274E-4 0.0 0.0 0.18484893345539105 1.0070767281688425E-4 34-35 3.0212301845065274E-4 0.0 0.0 0.35776400768198124 2.014153456337685E-4 36-37 3.0212301845065274E-4 0.0 5.0353836408442124E-5 0.6045985137561646 2.517691820422106E-4 38-39 3.0212301845065274E-4 0.0 1.0070767281688425E-4 0.89564368819696 3.0212301845065274E-4 40-41 3.0212301845065274E-4 0.0 1.0070767281688425E-4 1.191976015460642 3.0212301845065274E-4 42-43 3.5247685485909487E-4 0.0 1.0070767281688425E-4 1.5076945697415742 3.0212301845065274E-4 44-45 4.02830691267537E-4 0.0 1.0070767281688425E-4 1.9006055552366479 3.0212301845065274E-4 46-47 5.035383640844212E-4 0.0 1.0070767281688425E-4 2.3663785420147376 3.0212301845065274E-4 48-49 6.042460369013055E-4 0.0 1.0070767281688425E-4 2.7994718889637484 3.0212301845065274E-4 50-51 6.042460369013055E-4 0.0 1.0070767281688425E-4 3.235183635405998 3.0212301845065274E-4 52-53 6.042460369013055E-4 0.0 1.0070767281688425E-4 3.670140074302121 3.0212301845065274E-4 54-55 6.042460369013055E-4 0.0 1.0070767281688425E-4 4.161190686957248 3.0212301845065274E-4 56-57 7.049537097181897E-4 0.0 1.0070767281688425E-4 4.766393446750314 3.0212301845065274E-4 58-59 7.049537097181897E-4 0.0 1.0070767281688425E-4 5.376329467165774 3.0212301845065274E-4 60-61 7.049537097181897E-4 0.0 1.0070767281688425E-4 6.008824006292215 3.0212301845065274E-4 62-63 7.049537097181897E-4 0.0 1.0070767281688425E-4 6.636736346305488 3.0212301845065274E-4 64-65 7.049537097181897E-4 0.0 1.0070767281688425E-4 7.307399093429529 3.0212301845065274E-4 66-67 8.05661382535074E-4 0.0 1.0070767281688425E-4 8.045384919831656 3.0212301845065274E-4 68-69 8.05661382535074E-4 0.0 1.0070767281688425E-4 8.860966008139194 3.0212301845065274E-4 70-71 8.05661382535074E-4 0.0 1.0070767281688425E-4 9.723376164306583 3.0212301845065274E-4 72-73 9.063690553519582E-4 0.0 1.0070767281688425E-4 10.562321432707638 3.0212301845065274E-4 74-75 0.0010070767281688425 0.0 1.0070767281688425E-4 11.45882113612354 3.0212301845065274E-4 76-77 0.0010070767281688425 0.0 1.0070767281688425E-4 12.354615885829725 3.0212301845065274E-4 78-79 0.0010070767281688425 0.0 1.0070767281688425E-4 13.413254942480812 3.0212301845065274E-4 80-81 0.0010070767281688425 0.0 1.0070767281688425E-4 14.51877342082816 3.0212301845065274E-4 82-83 0.0010070767281688425 0.0 1.0070767281688425E-4 15.601582318955298 3.0212301845065274E-4 84-85 0.0010070767281688425 0.0 1.0070767281688425E-4 16.6978860452399 3.0212301845065274E-4 86-87 0.0010070767281688425 0.0 1.0070767281688425E-4 17.809648399301892 3.0212301845065274E-4 88-89 0.0010070767281688425 0.0 1.0070767281688425E-4 18.964261868147474 3.0212301845065274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 5065 0.0 21.369043 12-13 CCGTTGT 120 0.0047994065 19.76566 9 GTACTTT 5540 0.0 19.23744 14-15 GTACATG 4555 0.0 18.997375 1 TACCTGG 450 0.0 18.979828 2 GTAAGGT 305 4.620233E-9 18.66868 4 TACATGG 4450 0.0 18.65994 2 GGTAAGG 280 3.529567E-8 18.640902 3 AGTACTT 5330 0.0 18.391727 12-13 CCGTATC 155 5.5715645E-9 18.391726 94-95 ACATGGG 4335 0.0 18.38876 3 AGTATCG 160 0.0011993713 17.793587 5 CATGGGA 2420 0.0 16.862242 4 GTAAGCG 155 1.11444024E-7 16.859081 94-95 CATGGGG 1525 0.0 16.801813 4 ACTTTTT 6500 0.0 16.65268 16-17 CCGATTA 135 9.53203E-6 15.824915 16-17 CAATACA 275 1.13889055E-5 15.528949 4 CCAATAC 370 5.016227E-8 15.389049 3 TACTTTT 6390 0.0 15.34122 14-15 >>END_MODULE