FastQCFastQC Report
Thu 26 May 2016
SRR938810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992973
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77680.7822972024415568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53770.5415051567363866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52970.5334485429110358No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46860.47191615481991955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.34381599499684284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29050.2925557895330487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24290.24461893727221184No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23910.24079204570517024No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22540.2269950945292571No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT22420.22578660245545448No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21010.21158682058827377No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20780.20927054411348545No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19780.19919977683179707No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19510.19648066966574118No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.18892759420447483No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17450.175734889065463No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16950.1706995054246188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.13585465062997687No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13020.1311213900075833No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC12590.12679096007645727No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC12290.12376972989195074No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11450.11531028537533246No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT11320.11400108562871296RNA PCR Primer, Index 23 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.11077844009857267No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT10280.103527487655757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC1050.00221184722.59118
GGGCGCA1302.965807E-421.8959889
GAGTACT52650.021.31458912-13
GTATAGT2201.3208701E-619.4676071
CTCCGAC2350.019.17840294-95
TAAGGTG3506.548362E-1118.982265
ATGTCGT1250.006073792518.9765246
TACCTAT1507.784501E-418.9765246
GTACTTT57550.018.96385814-15
GTACATG43800.018.3613821
CCCTATA2101.9711424E-518.0783422
ACATGGG41750.017.9592633
TACATGG43450.017.9119152
GTAGGAC1600.001198343917.7958683
GTATATG3259.787982E-917.5707631
AGTACTT55700.017.5065612-13
TACAGTC2202.9476598E-517.2513857
TCCTACA3101.12793714E-716.83912
ATGGGAG8550.016.6511065
TAAGGCG2858.663683E-716.6511065
Produced by FastQC (version 0.11.5)