##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992973 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965598258965752 31.0 31.0 34.0 30.0 34.0 2 32.15455405131861 33.0 31.0 34.0 30.0 34.0 3 32.22271904674145 34.0 31.0 34.0 30.0 34.0 4 35.859993172019784 37.0 35.0 37.0 35.0 37.0 5 35.75756541215119 37.0 35.0 37.0 33.0 37.0 6 35.81041881299895 37.0 35.0 37.0 33.0 37.0 7 35.73163217932411 37.0 35.0 37.0 33.0 37.0 8 35.740960731057136 37.0 35.0 37.0 33.0 37.0 9 37.35067821582258 39.0 37.0 39.0 34.0 39.0 10-11 37.27998646488877 39.0 37.0 39.0 33.0 39.0 12-13 37.21616247370271 39.0 37.0 39.0 33.0 39.0 14-15 38.347462116291176 40.0 38.0 41.0 33.0 41.0 16-17 38.29755491841168 40.0 38.0 41.0 33.0 41.0 18-19 38.46406548818548 40.0 38.0 41.0 33.5 41.0 20-21 38.46779620392498 40.0 38.0 41.0 34.0 41.0 22-23 38.38021627979814 40.0 38.0 41.0 34.0 41.0 24-25 38.27692797286532 40.0 38.0 41.0 33.0 41.0 26-27 38.047859307352766 40.0 37.0 41.0 33.0 41.0 28-29 37.85580625052242 40.0 37.0 41.0 33.0 41.0 30-31 37.635580725759915 40.0 37.0 41.0 32.5 41.0 32-33 37.41254444984909 40.0 36.0 41.0 32.0 41.0 34-35 37.30741621373391 40.0 36.0 41.0 32.0 41.0 36-37 37.13068582932265 40.0 36.0 41.0 31.0 41.0 38-39 36.949735793420366 40.0 35.5 41.0 31.0 41.0 40-41 36.78406411856113 39.5 35.0 41.0 31.0 41.0 42-43 36.55003358600888 39.0 35.0 41.0 30.0 41.0 44-45 36.250094916981624 39.0 35.0 41.0 30.0 41.0 46-47 35.857034380592424 39.0 35.0 41.0 28.5 41.0 48-49 35.47115329419833 39.0 35.0 41.0 27.0 41.0 50-51 35.179474668495516 39.0 35.0 40.0 26.0 41.0 52-53 34.962691331989895 38.5 34.5 40.0 26.0 41.0 54-55 34.696663454091905 38.0 34.0 40.0 24.5 41.0 56-57 34.38138499234118 38.0 34.0 40.0 23.5 41.0 58-59 34.05146262788615 38.0 34.0 40.0 23.0 41.0 60-61 33.6169931105881 37.0 33.0 40.0 22.0 41.0 62-63 33.242339419098 37.0 33.0 40.0 20.5 41.0 64-65 32.814117302283144 36.0 32.0 39.0 18.5 40.5 66-67 32.351890232664935 36.0 32.0 39.0 16.0 40.0 68-69 31.98373168253316 35.0 31.5 38.5 12.5 40.0 70-71 31.52561449304261 35.0 31.0 38.0 8.0 40.0 72-73 31.026508777177227 35.0 31.0 37.0 6.5 39.0 74-75 30.54274537172713 35.0 30.0 36.5 2.0 39.0 76-77 28.598573677229894 32.5 27.5 35.0 2.0 36.5 78-79 29.514464643046686 34.0 29.0 35.0 2.0 37.0 80-81 29.539940159500812 34.0 30.0 35.0 2.0 37.0 82-83 29.206649626928424 34.0 29.0 35.0 2.0 37.0 84-85 28.810634830957135 34.0 29.0 35.0 2.0 36.0 86-87 28.274946549402653 34.0 28.5 35.0 2.0 36.0 88-89 27.676796347936953 33.5 27.0 35.0 2.0 35.0 90-91 27.160119157318476 33.0 26.0 35.0 2.0 35.0 92-93 26.87948010670985 33.0 25.0 35.0 2.0 35.0 94-95 26.537869609747695 33.0 25.0 35.0 2.0 35.0 96-97 25.934333561939752 33.0 24.0 35.0 2.0 35.0 98-99 25.146077990035984 32.0 19.0 34.0 2.0 35.0 100-101 21.800880285768095 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07543543583798495 1101 2 0.12388115432676727 1101 3 -0.06500199875259227 1101 4 0.08531219805846746 1101 5 -0.08442800071605205 1101 6 0.12013776082142158 1101 7 0.26042015328545887 1101 8 0.160689267437931 1101 9 0.16614793043970622 1101 10-11 0.16041465678926414 1101 12-13 0.058379132630719255 1101 14-15 0.28772984058758766 1101 16-17 0.25024922368266544 1101 18-19 0.23113553927202446 1101 20-21 0.12130082858586633 1101 22-23 0.10389770383062569 1101 24-25 0.09720970713127741 1101 26-27 0.2236119566450938 1101 28-29 0.19579520508682435 1101 30-31 0.16769797430050204 1101 32-33 0.3011350214006825 1101 34-35 0.28269230114434407 1101 36-37 0.19445008919701223 1101 38-39 0.18107183190196707 1101 40-41 0.29131640454308894 1101 42-43 0.30973251107600674 1101 44-45 0.13052801299647143 1101 46-47 0.03941690204162285 1101 48-49 0.053508653652997396 1101 50-51 0.31445574794652487 1101 52-53 0.0921203319080135 1101 54-55 0.019893816423433464 1101 56-57 0.17391330610938383 1101 58-59 0.09098243446111809 1101 60-61 0.14101742129097516 1101 62-63 0.10556117091066852 1101 64-65 0.1593520377547435 1101 66-67 0.06881205896858233 1101 68-69 -0.033397266524417546 1101 70-71 0.26444643232174414 1101 72-73 0.040385043688122124 1101 74-75 -0.28942769061693596 1101 76-77 -0.2553322914988634 1101 78-79 -0.2651606493109071 1101 80-81 -0.022149622036156558 1101 82-83 -0.13277245819287842 1101 84-85 0.026810404590879244 1101 86-87 0.15313932012397302 1101 88-89 0.16489129930618773 1101 90-91 0.17554863887240302 1101 92-93 0.03460742199739286 1101 94-95 -0.09191316845235775 1101 96-97 -0.17879646959778484 1101 98-99 -0.4478016875856703 1101 100-101 0.11047415837207453 1102 1 0.06041127158084336 1102 2 0.005170359425065385 1102 3 -0.015572808882026834 1102 4 -0.019062211061616097 1102 5 -0.1909013993308335 1102 6 -0.29937030504888895 1102 7 -0.09144064403151475 1102 8 -0.15976489638216407 1102 9 0.016867797576544774 1102 10-11 -0.10868902294059524 1102 12-13 -0.04934905915596488 1102 14-15 -0.12141784316312965 1102 16-17 0.041938030375497704 1102 18-19 -0.08412693217596967 1102 20-21 -0.08055180279937701 1102 22-23 0.06217496826649693 1102 24-25 0.10572700395484702 1102 26-27 0.020982411614006935 1102 28-29 -0.0060418995268136655 1102 30-31 -0.14504283442577304 1102 32-33 -0.12211097096705004 1102 34-35 0.029501837112391627 1102 36-37 -0.037993732643059275 1102 38-39 0.05992218372711733 1102 40-41 0.15394645563191744 1102 42-43 0.09013853040281106 1102 44-45 0.006416201838817415 1102 46-47 0.1466511124650367 1102 48-49 -0.20311495210042096 1102 50-51 -0.13505234092641416 1102 52-53 -0.24914948197776 1102 54-55 -0.4898046651733168 1102 56-57 -0.35136427806296666 1102 58-59 -0.4225333326879124 1102 60-61 -0.5210320435529567 1102 62-63 -0.7107795485654336 1102 64-65 -0.6230086295973933 1102 66-67 -0.5003712098423918 1102 68-69 -0.4299377867977796 1102 70-71 -0.3924901241393499 1102 72-73 -0.37354143532313344 1102 74-75 -0.3754483055691047 1102 76-77 -0.3566611531219728 1102 78-79 -0.4399271151646573 1102 80-81 -0.41684935727594663 1102 82-83 -0.20722102777466844 1102 84-85 0.0030245406238016415 1102 86-87 -0.19225083889316963 1102 88-89 -0.34321310040581565 1102 90-91 -0.23756067189995278 1102 92-93 -0.25506750491176433 1102 94-95 -0.3379699435303962 1102 96-97 -0.24928398722354572 1102 98-99 -0.2336524281551675 1102 100-101 0.24046486531173983 1103 1 0.17156537466899024 1103 2 0.07895451324374392 1103 3 0.0344400887110794 1103 4 0.06602883271744986 1103 5 -0.08546192211327508 1103 6 0.016018838484782805 1103 7 0.03409262183748751 1103 8 0.11851224974425634 1103 9 0.041161571676852304 1103 10-11 0.17917037345204534 1103 12-13 0.14499451949620124 1103 14-15 0.024288581161066247 1103 16-17 0.05332263660206138 1103 18-19 -0.021643054167348907 1103 20-21 0.1586125654500563 1103 22-23 0.20131188779213005 1103 24-25 0.294205484816807 1103 26-27 0.18013044291216573 1103 28-29 0.018240299810443616 1103 30-31 0.14594174798984483 1103 32-33 0.16048852238903066 1103 34-35 0.2891022762919775 1103 36-37 0.14075367957062923 1103 38-39 0.13940277450301863 1103 40-41 0.22630029944537 1103 42-43 0.30911485835883923 1103 44-45 0.3602633223684748 1103 46-47 0.1459417448439453 1103 48-49 0.4017309051265343 1103 50-51 0.17637492656966458 1103 52-53 0.19319993035498584 1103 54-55 0.15819852284713676 1103 56-57 -0.05746120182165981 1103 58-59 0.17685585053066433 1103 60-61 0.2771586556288739 1103 62-63 0.23196340571094964 1103 64-65 0.24500269382794215 1103 66-67 0.3000616862956065 1103 68-69 0.08645042414070048 1103 70-71 0.09945384077919783 1103 72-73 0.13063209352448268 1103 74-75 0.3515923251562718 1103 76-77 0.4391765620859438 1103 78-79 0.051710945301902456 1103 80-81 0.3166334558544648 1103 82-83 0.38022299997830444 1103 84-85 0.19810890411104864 1103 86-87 0.15200245660722445 1103 88-89 0.17276834028840327 1103 90-91 0.2129340665434718 1103 92-93 0.18458002701804332 1103 94-95 0.03742938579476274 1103 96-97 0.04465712038206604 1103 98-99 0.479516675825888 1103 100-101 0.9791712036519833 1104 1 0.15525958096302261 1104 2 0.25218962562499314 1104 3 0.24270262057174818 1104 4 0.07974286342383152 1104 5 -0.04568329471781851 1104 6 0.06946644110401223 1104 7 0.10641954441929613 1104 8 0.11490566563639959 1104 9 0.14532448371996765 1104 10-11 0.21743374205738775 1104 12-13 0.1739131664586111 1104 14-15 0.10168317278674976 1104 16-17 0.2367209785603066 1104 18-19 0.2899123898222413 1104 20-21 0.27650238119458237 1104 22-23 0.3478012074972483 1104 24-25 0.3881268565631544 1104 26-27 0.41842674492978915 1104 28-29 0.26341292667381566 1104 30-31 0.22625153661527264 1104 32-33 0.25174411141438213 1104 34-35 0.29651658505505196 1104 36-37 0.5176181183509598 1104 38-39 0.2393562171709931 1104 40-41 0.36117764125771856 1104 42-43 0.3404203731499109 1104 44-45 0.24663285885483788 1104 46-47 0.003485963581880469 1104 48-49 0.09315770498579212 1104 50-51 0.020841791480449956 1104 52-53 0.005673647990136033 1104 54-55 -0.035544343368123066 1104 56-57 -0.0412626830742866 1104 58-59 0.136794017244533 1104 60-61 0.39630358838892477 1104 62-63 0.17297183323169918 1104 64-65 0.06519323560938517 1104 66-67 0.18480386166012863 1104 68-69 0.11422927466806954 1104 70-71 0.04886136118289386 1104 72-73 0.2989298759365404 1104 74-75 0.3724680320982472 1104 76-77 0.41548488748092893 1104 78-79 0.2825312554552646 1104 80-81 0.33054058148631427 1104 82-83 0.3031303873610547 1104 84-85 0.5042308714479695 1104 86-87 0.5727734631896126 1104 88-89 0.6695103997959713 1104 90-91 0.48211107451614765 1104 92-93 0.694578053158807 1104 94-95 0.6584757671958599 1104 96-97 0.7813239157786818 1104 98-99 1.2581730355145346 1104 100-101 1.8108224656345975 1105 1 -0.009503223104861291 1105 2 0.031630207563509316 1105 3 -0.07505406662377112 1105 4 0.04927549092134598 1105 5 -0.08057850567470837 1105 6 -0.04481649766876927 1105 7 -0.11124125328102963 1105 8 -0.07395903616607313 1105 9 0.08637333513512147 1105 10-11 0.014056614313361138 1105 12-13 -0.0596921323238675 1105 14-15 0.04569749562725178 1105 16-17 0.03386246883765409 1105 18-19 0.14159808118759543 1105 20-21 -0.06544185666150071 1105 22-23 -0.06373498817581691 1105 24-25 0.023186897175172305 1105 26-27 0.0598961313205848 1105 28-29 -0.012901323466152803 1105 30-31 0.09624240726682132 1105 32-33 0.13626620986927662 1105 34-35 0.17709081480544597 1105 36-37 0.21323582218402493 1105 38-39 0.447416575325569 1105 40-41 0.1592816824045471 1105 42-43 0.19797172312358668 1105 44-45 0.2271285386048163 1105 46-47 0.28439935802822447 1105 48-49 0.19856198679644876 1105 50-51 0.09225805216618 1105 52-53 0.2914483682602125 1105 54-55 0.17627325350324696 1105 56-57 0.29849113788184667 1105 58-59 0.21411040661855196 1105 60-61 0.006408335650121444 1105 62-63 -0.15988692718244835 1105 64-65 -0.12495573780895342 1105 66-67 -0.03619896697552605 1105 68-69 -0.0032081986959653364 1105 70-71 -0.058344243443197996 1105 72-73 0.023295104191142002 1105 74-75 -0.05176464303064776 1105 76-77 0.05671507240768747 1105 78-79 -0.035693389189546565 1105 80-81 -0.07628574780531494 1105 82-83 0.012929395458389337 1105 84-85 0.08748760460358795 1105 86-87 0.3745433451139242 1105 88-89 0.1226175119135604 1105 90-91 0.21640159670652181 1105 92-93 0.34213924277349506 1105 94-95 0.4821200485051378 1105 96-97 0.16901099430361555 1105 98-99 0.37996627352939427 1105 100-101 1.0422650280358852 1106 1 0.21436250958359793 1106 2 0.1863795922189908 1106 3 0.10913145252214207 1106 4 0.05286056363189573 1106 5 0.029120662608704606 1106 6 0.1785198409730384 1106 7 0.15734712053607325 1106 8 0.21225349424619822 1106 9 0.23297897612279428 1106 10-11 0.225574382988448 1106 12-13 0.21606182317255218 1106 14-15 0.22172304446791458 1106 16-17 0.27057564172334736 1106 18-19 0.06419702057400656 1106 20-21 0.05530055256820532 1106 22-23 0.1825067704671568 1106 24-25 0.10566790947395077 1106 26-27 0.16354225675451772 1106 28-29 0.13431281642571946 1106 30-31 0.19211140962271855 1106 32-33 0.21011820062415154 1106 34-35 0.3145826634555462 1106 36-37 0.2129427285451797 1106 38-39 0.3765477504485162 1106 40-41 0.45099098625137657 1106 42-43 0.47016342461837013 1106 44-45 0.26772454299817383 1106 46-47 0.19889968408222103 1106 48-49 0.22173869589239104 1106 50-51 0.3985601739004423 1106 52-53 0.3968378430546693 1106 54-55 0.25459466870943714 1106 56-57 0.32380773479088276 1106 58-59 0.22078462543273503 1106 60-61 0.02014722674200442 1106 62-63 0.26686197473057405 1106 64-65 0.2993880721852378 1106 66-67 0.25665837147055015 1106 68-69 0.32351311793339477 1106 70-71 0.30946393336224176 1106 72-73 0.32331346057474875 1106 74-75 0.3118854579294599 1106 76-77 0.22859674371077787 1106 78-79 0.16420002208573337 1106 80-81 0.2265053352517441 1106 82-83 0.2876909906585503 1106 84-85 0.4220981095258374 1106 86-87 0.3306213871267154 1106 88-89 0.3976980619560031 1106 90-91 0.09641524736527529 1106 92-93 0.26513269228279057 1106 94-95 0.5848473405937398 1106 96-97 0.6962474052553773 1106 98-99 0.8619917229818945 1106 100-101 1.527377088179918 1107 1 0.11038124307490804 1107 2 0.044363903719229825 1107 3 -0.017846093662228668 1107 4 0.06329625317062693 1107 5 -0.03929973803546005 1107 6 -0.04369660917421925 1107 7 0.11606690538864228 1107 8 0.12964011626364425 1107 9 0.16985034720816827 1107 10-11 0.040544192816334146 1107 12-13 0.1669513443256463 1107 14-15 0.13477395017307714 1107 16-17 0.18713008593247338 1107 18-19 0.22457456755758898 1107 20-21 0.2266028059418801 1107 22-23 0.17817804724570152 1107 24-25 0.18186512662340704 1107 26-27 0.037800489620444466 1107 28-29 0.15487600000034263 1107 30-31 0.21523156690732748 1107 32-33 0.13663430271529364 1107 34-35 0.1598710185607004 1107 36-37 0.3539998640952504 1107 38-39 0.15522991247242857 1107 40-41 0.11066522810042301 1107 42-43 0.21398602788634236 1107 44-45 0.25303905226302703 1107 46-47 0.1446897435035126 1107 48-49 0.3419394375596241 1107 50-51 0.1984269055695549 1107 52-53 0.18482481192942402 1107 54-55 0.2072489462831939 1107 56-57 0.19062382049819604 1107 58-59 0.24608412952269276 1107 60-61 0.3715060841908482 1107 62-63 0.2834061291060479 1107 64-65 0.274827837696904 1107 66-67 0.27638588069144276 1107 68-69 0.3195155675132142 1107 70-71 0.18413226105448288 1107 72-73 0.23280388403637176 1107 74-75 0.2716152448999338 1107 76-77 0.30814564939196387 1107 78-79 0.2716903216697446 1107 80-81 0.35228006981399673 1107 82-83 0.22471471122263154 1107 84-85 0.15834170085760135 1107 86-87 0.2541059843446618 1107 88-89 0.2498995868494056 1107 90-91 0.5302840849537347 1107 92-93 0.4244807563954467 1107 94-95 0.5765904983538732 1107 96-97 0.6908387490077494 1107 98-99 0.8751966764878318 1107 100-101 1.487154328266712 1108 1 0.27940579562393353 1108 2 0.16000545897326646 1108 3 0.13367580140392477 1108 4 0.11889598007645219 1108 5 0.10830507049613658 1108 6 0.18625127823493415 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Count 2 1.0 3 9.0 4 6.0 5 47.0 6 148.0 7 336.0 8 580.0 9 1117.0 10 1897.0 11 2755.0 12 3781.0 13 5018.0 14 5879.0 15 7451.0 16 9345.0 17 12346.0 18 11751.0 19 8110.0 20 7612.0 21 7572.0 22 8348.0 23 8925.0 24 10077.0 25 11424.0 26 12765.0 27 15489.0 28 18514.0 29 22414.0 30 26762.0 31 31985.0 32 39101.0 33 48098.0 34 64064.0 35 88902.0 36 130349.0 37 198253.0 38 157547.0 39 14195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6178029496286 20.887735048213337 15.627419372747175 20.867042629410893 2 15.505859675942851 23.474757118270084 37.74674638686047 23.2726368189266 3 20.668537815227605 25.82144731024912 32.05636004201524 21.453654832508036 4 12.873763939200764 18.73424554343371 38.09489281178844 30.29709770557709 5 12.152824997909722 39.29399984486613 34.75089428633885 13.8022808708853 6 28.404608128781067 37.83940838378111 17.558646485541438 16.197337001896386 7 24.73410656684522 35.24254939459583 22.208660255616213 17.81468378294274 8 24.33278649066994 33.01147161101057 20.881937373926583 21.773804524392908 9 23.92673315387226 18.832636939775803 23.093075038294092 34.147554868057846 10-11 23.859963966794666 26.806771181089516 27.989079259959738 21.34418559215608 12-13 26.535968248884917 25.06060084211756 27.69385471709704 20.709576191900485 14-15 22.74734559751373 27.194294306088885 28.165921933426187 21.8924381629712 16-17 19.940489828433655 29.862393428804072 29.161192600354806 21.035924142407467 18-19 19.556070507455892 29.46479914358195 30.77218615209074 20.206944196871415 20-21 21.335625439966645 27.42320284640166 29.9512172032875 21.289954510344188 22-23 20.535956163964176 27.513185152063553 30.345084911674334 21.605773772297937 24-25 21.787778980119732 27.856495088721257 30.23698109559256 20.11874483556645 26-27 21.24410852400902 26.84463623912343 31.86840356106993 20.042851675797614 28-29 19.89399510359295 28.94212632166232 31.097270519943642 20.066608054801087 30-31 20.557960790474667 27.925583072248696 30.430182895204606 21.08627324207204 32-33 20.650057957265705 27.954838651201996 30.6401080391914 20.7549953523409 34-35 21.829832662742156 27.411786626112267 30.405577572879583 20.352803138265998 36-37 21.565193918031113 26.71606214119914 31.460001903699975 20.258742037069773 38-39 20.684082477071936 27.53966299485261 31.37041608688667 20.405838441188777 40-41 20.451955436956226 27.978553418294457 31.119751471846453 20.449739672902865 42-43 20.11032379540445 28.354796652819957 29.82131553622713 21.713564015548464 44-45 20.58890176880011 27.514494935710665 29.863495321436595 22.03310797405263 46-47 21.646327800255296 28.074634869721315 29.372663254336118 20.90637407568727 48-49 21.197335226671903 27.73323060964495 29.729997834745785 21.339436328937364 50-51 19.90482117842076 28.27670037352476 30.63134647165633 21.18713197639815 52-53 20.419336678842225 28.940968183424925 29.29208548470099 21.347609653031853 54-55 19.92722863562252 28.114208543434714 29.39928880241457 22.559274018528196 56-57 20.850078377127677 28.023813756004174 28.769818989874167 22.356288876993986 58-59 21.40362820811717 27.629398486056218 29.001293594616957 21.96567971120966 60-61 20.729717059277615 28.14522232567414 29.80014532505024 21.324915289998 62-63 19.96171067287299 28.272770668884938 29.81607628055744 21.949442377684633 64-65 19.697589974988833 29.258683537496356 29.061998184210484 21.981728303304322 66-67 20.025631728958317 28.484605855515603 28.27033668711162 23.219425728414457 68-69 19.839965034207964 28.23125158300272 28.658504960620686 23.270278422168634 70-71 20.765468950313856 28.054337831945077 28.05202155547029 23.128171662270777 72-73 20.44401005868236 27.323754019494988 29.08764890888272 23.14458701293993 74-75 19.802732519173606 29.008632214681967 28.028639435469373 23.159995830675054 76-77 20.036749146516943 27.93867215367935 28.29956348422618 23.72501521557753 78-79 20.122773693109135 28.018148803854046 28.38090301291726 23.47817449011956 80-81 20.73859974218498 27.728861182726394 27.867235336770868 23.665303738317757 82-83 20.397808783697705 27.4025909284773 28.530828644931418 23.668771642893578 84-85 20.223661670559018 27.59606756679185 28.35605801970446 23.824212742944674 86-87 20.126169449149124 27.723405113315593 28.059873780143562 24.090551657391725 88-89 20.034552263203558 27.5662685425342 27.48916009905858 24.910019095203662 90-91 20.053256851587975 27.116512505200074 27.654388509136712 25.17584213407524 92-93 20.483409821273014 27.441798166505034 27.522717270563874 24.552074741658082 94-95 20.316102675966054 27.02230735416078 27.719560086749983 24.942029883123183 96-97 19.686274825750196 26.87555829928568 28.275397770931882 25.16276910403224 98-99 20.00411894381821 27.160104051167554 27.322746942766823 25.51303006224741 100-101 19.96385570682537 26.425214597299302 27.960063023401137 25.650866672474194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2112.0 1 1428.5 2 778.0 3 793.0 4 674.5 5 674.5 6 995.0 7 1700.5 8 2340.0 9 2539.0 10 2258.5 11 1840.0 12 1782.5 13 1841.0 14 1942.0 15 2006.0 16 2075.0 17 2234.5 18 2358.5 19 2515.5 20 2780.5 21 3103.0 22 3615.5 23 4300.5 24 4895.0 25 5693.5 26 7191.5 27 9094.5 28 10304.5 29 11523.0 30 13169.0 31 14960.5 32 17190.0 33 19114.5 34 21020.0 35 22978.0 36 25655.0 37 27860.5 38 28135.0 39 27794.5 40 27723.0 41 28035.0 42 28161.0 43 28925.0 44 30693.0 45 33100.5 46 37177.5 47 45676.0 48 55741.0 49 55039.5 50 45579.0 51 37758.5 52 31884.5 53 27953.5 54 25282.0 55 22650.0 56 20119.5 57 17759.0 58 16186.5 59 14588.5 60 12064.5 61 9636.5 62 7568.0 63 5489.0 64 3765.0 65 2652.5 66 1931.0 67 1525.5 68 1210.5 69 879.5 70 605.0 71 402.0 72 294.5 73 224.0 74 153.0 75 121.5 76 80.5 77 44.5 78 31.5 79 18.5 80 9.5 81 6.0 82 5.0 83 1.5 84 2.0 85 3.5 86 3.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.228807832639961 2 0.0 3 0.0 4 0.0 5 0.02839956373436136 6 0.0032226455301402955 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003373707039365622 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.5247685485909487E-4 26-27 0.0013091997466194952 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006848121751548129 36-37 0.017069950542461878 38-39 0.01404872035795535 40-41 0.008811921371477372 42-43 0.007804844643308529 44-45 0.00402830691267537 46-47 5.538922004928634E-4 48-49 0.0018127381107039165 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0017623842742954744 58-59 3.5247685485909487E-4 60-61 0.0026687533296474325 62-63 7.049537097181897E-4 64-65 0.0016616766014785902 66-67 0.006344583387463708 68-69 2.517691820422106E-4 70-71 0.0 72-73 0.0 74-75 6.545998733097476E-4 76-77 0.05730266583280713 78-79 0.14023543439751132 80-81 0.0013091997466194952 82-83 8.560152189435161E-4 84-85 0.0 86-87 0.10604517947617911 88-89 0.3481967787643773 90-91 0.3831423412318361 92-93 6.042460369013055E-4 94-95 4.531845276759791E-4 96-97 2.0141534563376847E-4 98-99 0.0 100-101 8.560152189435161E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 992973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.42757990712715 #Duplication Level Percentage of deduplicated Percentage of total 1 80.49761769939701 44.61788137366701 2 11.412475064516782 12.651317471532 3 3.3264077765017115 5.531241985072134 4 1.4940720433295591 3.3125119027461554 5 0.7818821008866195 2.1668916312422777 6 0.4648842834834038 1.546044646220635 7 0.33126166495111137 1.2852722682971995 8 0.2368897172362747 1.0504178985032298 9 0.17686409580230833 0.8822833922505814 >10 1.1324305476154568 11.881547098836947 >50 0.09584623794522275 3.685770032618016 >100 0.04247166120977336 4.178630518176075 >500 0.0023595366479606692 0.999889440934102 >1k 0.0039930620196257476 4.354986820464468 >5k 5.445084572216929E-4 1.8553135194392505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7768 0.7822972024415568 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5377 0.5415051567363866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5297 0.5334485429110358 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4686 0.47191615481991955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3414 0.34381599499684284 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2905 0.2925557895330487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2429 0.24461893727221184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2391 0.24079204570517024 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2254 0.2269950945292571 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2242 0.22578660245545448 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2101 0.21158682058827377 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2078 0.20927054411348545 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1978 0.19919977683179707 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1951 0.19648066966574118 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1876 0.18892759420447483 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1745 0.175734889065463 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1695 0.1706995054246188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1349 0.13585465062997687 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1302 0.1311213900075833 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1259 0.12679096007645727 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1229 0.12376972989195074 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1145 0.11531028537533246 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1132 0.11400108562871296 RNA PCR Primer, Index 23 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1100 0.11077844009857267 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1028 0.103527487655757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.0212301845065274E-4 0.0 0.0 0.0 0.0 8 3.0212301845065274E-4 0.0 0.0 0.0 0.0 9 3.0212301845065274E-4 0.0 0.0 0.0 0.0 10-11 3.0212301845065274E-4 0.0 0.0 1.5106150922532637E-4 0.0 12-13 3.5247685485909487E-4 0.0 0.0 2.517691820422106E-4 0.0 14-15 4.02830691267537E-4 0.0 0.0 4.02830691267537E-4 0.0 16-17 4.02830691267537E-4 0.0 0.0 4.531845276759791E-4 0.0 18-19 4.02830691267537E-4 0.0 0.0 5.538922004928634E-4 0.0 20-21 4.02830691267537E-4 0.0 0.0 0.001208492073802611 0.0 22-23 4.02830691267537E-4 0.0 0.0 0.003625476221407833 0.0 24-25 4.02830691267537E-4 0.0 0.0 0.006545998733097476 0.0 26-27 4.02830691267537E-4 0.0 0.0 0.012538105265702088 0.0 28-29 4.02830691267537E-4 0.0 0.0 0.032629285992670495 0.0 30-31 4.02830691267537E-4 0.0 0.0 0.08318453774674639 0.0 32-33 4.02830691267537E-4 0.0 0.0 0.18822264049475665 1.0070767281688425E-4 34-35 4.531845276759791E-4 0.0 0.0 0.3639071757238112 1.0070767281688425E-4 36-37 6.042460369013055E-4 0.0 0.0 0.6160288346208809 1.0070767281688425E-4 38-39 7.049537097181897E-4 0.0 0.0 0.9113037313199855 1.0070767281688425E-4 40-41 7.553075461266319E-4 0.0 0.0 1.2115133039871175 1.0070767281688425E-4 42-43 9.063690553519582E-4 0.0 0.0 1.529648842415655 1.0070767281688425E-4 44-45 9.063690553519582E-4 0.0 0.0 1.926235657968545 1.0070767281688425E-4 46-47 9.063690553519582E-4 0.0 0.0 2.3979000436064224 1.0070767281688425E-4 48-49 9.063690553519582E-4 0.0 0.0 2.8372372662700798 1.0070767281688425E-4 50-51 9.063690553519582E-4 0.0 0.0 3.2798474883002857 1.0070767281688425E-4 52-53 9.063690553519582E-4 0.0 0.0 3.7217527566207744 1.0070767281688425E-4 54-55 0.0011077844009857267 0.0 0.0 4.217637337571112 1.0070767281688425E-4 56-57 0.0011077844009857267 0.0 0.0 4.828177604023473 1.0070767281688425E-4 58-59 0.001208492073802611 0.0 0.0 5.4467744843011845 1.0070767281688425E-4 60-61 0.001208492073802611 0.0 0.0 6.084455468577696 1.0070767281688425E-4 62-63 0.0015106150922532637 0.0 0.0 6.720827253107586 1.0070767281688425E-4 64-65 0.0015106150922532637 0.0 0.0 7.396978568400147 1.0070767281688425E-4 66-67 0.0015106150922532637 0.0 0.0 8.143373485482485 1.0070767281688425E-4 68-69 0.0015106150922532637 0.0 0.0 8.969881356290655 1.0070767281688425E-4 70-71 0.0015106150922532637 0.0 0.0 9.839592818737266 1.0070767281688425E-4 72-73 0.0015106150922532637 0.0 0.0 10.6873500085098 1.0070767281688425E-4 74-75 0.0015106150922532637 0.0 0.0 11.591805618078237 1.0070767281688425E-4 76-77 0.0015106150922532637 0.0 0.0 12.49958458084963 1.0070767281688425E-4 78-79 0.0015106150922532637 0.0 0.0 13.570509973584377 1.0070767281688425E-4 80-81 0.001611322765070148 0.0 0.0 14.68549497317651 1.0070767281688425E-4 82-83 0.0017120304378870322 0.0 0.0 15.778928530785832 2.014153456337685E-4 84-85 0.0017120304378870322 0.0 0.0 16.882332148004025 2.014153456337685E-4 86-87 0.0017120304378870322 0.0 0.0 18.00144616218165 2.014153456337685E-4 88-89 0.0017120304378870322 0.0 0.0 19.168043844092438 2.014153456337685E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGCGC 105 0.002211847 22.5911 8 GGGCGCA 130 2.965807E-4 21.895988 9 GAGTACT 5265 0.0 21.314589 12-13 GTATAGT 220 1.3208701E-6 19.467607 1 CTCCGAC 235 0.0 19.178402 94-95 TAAGGTG 350 6.548362E-11 18.98226 5 ATGTCGT 125 0.0060737925 18.976524 6 TACCTAT 150 7.784501E-4 18.976524 6 GTACTTT 5755 0.0 18.963858 14-15 GTACATG 4380 0.0 18.361382 1 CCCTATA 210 1.9711424E-5 18.078342 2 ACATGGG 4175 0.0 17.959263 3 TACATGG 4345 0.0 17.911915 2 GTAGGAC 160 0.0011983439 17.795868 3 GTATATG 325 9.787982E-9 17.570763 1 AGTACTT 5570 0.0 17.50656 12-13 TACAGTC 220 2.9476598E-5 17.251385 7 TCCTACA 310 1.12793714E-7 16.8391 2 ATGGGAG 855 0.0 16.651106 5 TAAGGCG 285 8.663683E-7 16.651106 5 >>END_MODULE