##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388312 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.388568985764024 33.0 31.0 34.0 30.0 34.0 2 31.361989843218854 33.0 31.0 34.0 28.0 34.0 3 31.30538845052432 33.0 31.0 34.0 28.0 34.0 4 34.891625806052865 37.0 35.0 37.0 32.0 37.0 5 34.94873967325243 37.0 35.0 37.0 32.0 37.0 6 35.087298358021386 37.0 35.0 37.0 32.0 37.0 7 35.03885792867591 37.0 35.0 37.0 32.0 37.0 8 35.03265672964008 37.0 35.0 37.0 32.0 37.0 9 36.51116112816498 39.0 37.0 39.0 32.0 39.0 10-11 36.41959043243577 39.0 37.0 39.0 32.0 39.0 12-13 36.282526679577245 39.0 37.0 39.0 32.0 39.0 14-15 37.20577396526504 40.0 37.0 41.0 32.0 41.0 16-17 37.18723603700117 40.0 37.0 41.0 32.0 41.0 18-19 37.26874781103855 40.0 37.0 41.0 32.0 41.0 20-21 37.23475710253611 40.0 37.0 41.0 32.0 41.0 22-23 37.07419677990894 40.0 36.0 41.0 32.0 41.0 24-25 36.9679523166938 40.0 36.0 41.0 31.0 41.0 26-27 36.74271204598364 39.5 35.5 41.0 31.0 41.0 28-29 36.50926574507098 39.0 35.0 41.0 30.5 41.0 30-31 36.12053194338573 38.5 35.0 41.0 30.0 41.0 32-33 35.65458960835617 38.0 35.0 40.0 30.0 41.0 34-35 35.19242130039761 38.0 35.0 40.0 28.0 41.0 36-37 34.78663548898824 38.0 35.0 40.0 25.0 41.0 38-39 34.44091477986774 38.0 35.0 40.0 24.5 41.0 40-41 34.05669796452337 37.5 34.5 40.0 22.5 41.0 42-43 33.630893456807925 37.0 34.0 40.0 19.5 41.0 44-45 33.13691825130307 37.5 33.0 40.0 10.0 41.0 46-47 32.49572508704341 37.0 33.0 40.0 2.0 41.0 48-49 31.929089237520344 37.0 32.5 40.0 2.0 41.0 50-51 30.65652619543048 36.0 30.0 39.0 2.0 40.0 52-53 30.665667040936153 35.5 30.5 39.5 2.0 40.0 54-55 31.075944086198724 37.0 31.0 40.0 2.0 41.0 56-57 30.928789221038752 36.0 31.0 40.0 2.0 41.0 58-59 30.688331805352398 36.0 31.0 40.0 2.0 41.0 60-61 30.361988555594472 35.5 30.0 40.0 2.0 41.0 62-63 29.937586270833762 35.0 29.0 39.5 2.0 41.0 64-65 29.48452404252251 35.0 28.0 39.0 2.0 41.0 66-67 29.048249860936565 35.0 27.5 39.0 2.0 40.5 68-69 28.624661354786873 35.0 26.0 38.0 2.0 40.0 70-71 28.153759090628157 35.0 26.0 37.5 2.0 40.0 72-73 27.671062187107275 35.0 24.5 37.0 2.0 39.0 74-75 27.190252940934094 34.5 23.0 36.0 2.0 39.0 76-77 26.684484383691462 34.0 20.5 36.0 2.0 38.5 78-79 26.217465594676447 34.0 18.0 35.0 2.0 37.5 80-81 25.745289870001443 34.0 9.5 35.0 2.0 37.0 82-83 25.232763859988875 33.5 2.0 35.0 2.0 36.5 84-85 24.754932889017077 33.0 2.0 35.0 2.0 36.0 86-87 24.288833463812605 33.0 2.0 35.0 2.0 36.0 88-89 23.84377639629988 33.0 2.0 35.0 2.0 35.0 90-91 23.350155287500773 32.0 2.0 35.0 2.0 35.0 92-93 22.85349667277859 32.0 2.0 35.0 2.0 35.0 94-95 22.316330682543935 31.5 2.0 35.0 2.0 35.0 96-97 21.684444467335545 31.0 2.0 35.0 2.0 35.0 98-99 21.02549625043779 31.0 2.0 35.0 2.0 35.0 100-101 19.340312686705538 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3418308212320511 1101 2 0.25028007308264577 1101 3 0.44604784854163526 1101 4 0.31631543947890606 1101 5 0.15836647073815868 1101 6 0.08544709291483343 1101 7 0.24525853715734058 1101 8 0.14682178943155577 1101 9 0.4733252531377161 1101 10-11 0.6473656607749589 1101 12-13 0.4284415293196133 1101 14-15 0.6084810301790355 1101 16-17 0.7532840765993001 1101 18-19 0.7838203486035482 1101 20-21 0.7030901950168555 1101 22-23 0.7150647389383877 1101 24-25 0.8452974260550334 1101 26-27 0.9583553055038578 1101 28-29 0.7909128190517691 1101 30-31 0.6770194343492122 1101 32-33 0.6636062311106272 1101 34-35 0.6350797666035959 1101 36-37 0.6737694656384292 1101 38-39 0.3585039417448641 1101 40-41 0.5064641912829231 1101 42-43 0.8319434358524305 1101 44-45 0.8434270749198518 1101 46-47 0.5110910202071537 1101 48-49 0.2455445093822206 1101 50-51 0.4553316359994035 1101 52-53 0.5580544586013367 1101 54-55 0.10933410463258753 1101 56-57 -0.09774491063615187 1101 58-59 -0.7041803462189868 1101 60-61 -1.0795430993826187 1101 62-63 -0.6477743124402373 1101 64-65 -0.7035363004805895 1101 66-67 -0.4976282225857851 1101 68-69 -0.45471799727579665 1101 70-71 -0.30525595585423915 1101 72-73 -0.349689711922732 1101 74-75 -0.6650330515588863 1101 76-77 -0.7897403961153806 1101 78-79 -0.8108226175117466 1101 80-81 -0.7514129918158581 1101 82-83 -0.8196565820168935 1101 84-85 -1.134229835120422 1101 86-87 -1.1513026388734744 1101 88-89 -0.8139040326193694 1101 90-91 -1.2018682704060346 1101 92-93 -1.5131710512365082 1101 94-95 -1.3283359713327378 1101 96-97 -1.3243740516517555 1101 98-99 -1.1883837132431943 1101 100-101 -0.9753142107095805 1102 1 0.1510560611620555 1102 2 0.03702291049524575 1102 3 0.05815774158346798 1102 4 -0.10957110482280541 1102 5 -0.1452675957909193 1102 6 0.13695651355156002 1102 7 -0.06043935899201358 1102 8 -0.21963744797162832 1102 9 -0.010937065100101506 1102 10-11 -0.027553708619159067 1102 12-13 -0.07592154774833659 1102 14-15 -0.40657786351412994 1102 16-17 0.04860789382090047 1102 18-19 0.17456293490379693 1102 20-21 0.06618688111857551 1102 22-23 0.16947215303049745 1102 24-25 -0.05407714725015467 1102 26-27 -0.003071483475444836 1102 28-29 -0.07207400539715536 1102 30-31 0.2478331168333412 1102 32-33 0.005291665809643575 1102 34-35 0.004622977545814422 1102 36-37 0.12913032193154095 1102 38-39 -0.09954852504160527 1102 40-41 -0.5008023723230579 1102 42-43 -0.43452207935948906 1102 44-45 -0.20209488563980926 1102 46-47 -0.10390274122041632 1102 48-49 0.12650156132323787 1102 50-51 0.046403022531936955 1102 52-53 0.05621035228664795 1102 54-55 -0.07823504935421255 1102 56-57 -0.10962566365931181 1102 58-59 0.229522069952651 1102 60-61 0.17610496665993125 1102 62-63 0.35419142641861256 1102 64-65 0.19747835035553862 1102 66-67 -0.19257380183134742 1102 68-69 0.06914944679285284 1102 70-71 -0.0674525611291692 1102 72-73 -0.29493544889824364 1102 74-75 -0.4889169748754547 1102 76-77 -0.2531446948449094 1102 78-79 -0.1864356847706219 1102 80-81 -0.23269335090651566 1102 82-83 -0.1786491506608101 1102 84-85 -0.1614598304905357 1102 86-87 -0.17349469273707285 1102 88-89 -0.36789711133524605 1102 90-91 -0.753872068480316 1102 92-93 -0.45259412061414395 1102 94-95 -0.7554427647065083 1102 96-97 -0.3561868473953993 1102 98-99 -0.7710349888154546 1102 100-101 -0.6122920735865414 1103 1 0.15554524732815267 1103 2 0.21241646498480904 1103 3 0.0529403763904277 1103 4 0.3556377023899202 1103 5 0.3203616034373624 1103 6 0.2946335473856081 1103 7 0.22435371054532993 1103 8 0.2699015471413091 1103 9 0.0027632104068118224 1103 10-11 0.05714402201466129 1103 12-13 0.19497231077473742 1103 14-15 0.18741728696369364 1103 16-17 0.2362753937646005 1103 18-19 0.3003952735595945 1103 20-21 0.5056804588798371 1103 22-23 0.5635258581104807 1103 24-25 0.44868918235220434 1103 26-27 0.3671625097232152 1103 28-29 0.4058816882871952 1103 30-31 0.7424055556325229 1103 32-33 0.5069582198713007 1103 34-35 0.26464669966669163 1103 36-37 0.6501123877078783 1103 38-39 0.7983037221789218 1103 40-41 0.31880441100239665 1103 42-43 0.8634119941640748 1103 44-45 0.4413488756676074 1103 46-47 0.444626262075154 1103 48-49 0.18199706087656864 1103 50-51 0.26089678796820337 1103 52-53 0.2780234429937316 1103 54-55 0.5268817220351067 1103 56-57 0.3099030468632513 1103 58-59 0.2636601537157581 1103 60-61 0.3407222222507684 1103 62-63 0.3669120383892768 1103 64-65 0.2979070000713975 1103 66-67 0.19681430257601207 1103 68-69 -0.006934001022234071 1103 70-71 -0.17329375804529334 1103 72-73 -0.12602418914852365 1103 74-75 -0.12396040287206134 1103 76-77 -0.319761815309743 1103 78-79 -0.46988124775810647 1103 80-81 -1.0806390602805074 1103 82-83 -1.2090675157862876 1103 84-85 -1.0357476038391837 1103 86-87 -1.1432339746174272 1103 88-89 -1.1133159644157402 1103 90-91 -1.4567374904863364 1103 92-93 -0.879738682918493 1103 94-95 -1.0881492635166516 1103 96-97 -1.543801798337153 1103 98-99 -1.4937339055553167 1103 100-101 -1.079373877368571 1104 1 0.00590982832203224 1104 2 0.008266080939531406 1104 3 -0.19147112849810455 1104 4 -0.150433656666209 1104 5 -0.3987837648118955 1104 6 -0.18411295278657747 1104 7 -0.05764631939971565 1104 8 -0.03398831496173216 1104 9 -0.08412235458143869 1104 10-11 -0.1626526510901911 1104 12-13 -0.34579038966994347 1104 14-15 -0.27063718308376394 1104 16-17 -0.16332259392798676 1104 18-19 -0.5407574611721699 1104 20-21 -0.34898123690838645 1104 22-23 -0.28843329684018215 1104 24-25 -0.4917426252807928 1104 26-27 -0.29101473503266106 1104 28-29 -0.6589643192888985 1104 30-31 -0.4479551325047524 1104 32-33 -0.50623631815143 1104 34-35 -0.4572386407336779 1104 36-37 -0.11508846872057177 1104 38-39 -0.20545063856909707 1104 40-41 -0.101826231768122 1104 42-43 -0.41434332482557323 1104 44-45 -0.4517734947826213 1104 46-47 -0.6724776023336538 1104 48-49 -0.7298596820002459 1104 50-51 -0.3656659693720776 1104 52-53 -0.487177413434722 1104 54-55 -0.6375278364251216 1104 56-57 -0.45844952616523926 1104 58-59 -0.3087731811148302 1104 60-61 -0.35143516893640125 1104 62-63 -0.6768189652191019 1104 64-65 -0.953399352771136 1104 66-67 -1.093925939080549 1104 68-69 -0.8759730643410464 1104 70-71 -0.7801788738577748 1104 72-73 -0.40894502940665234 1104 74-75 -0.547214980721705 1104 76-77 -0.905969873522924 1104 78-79 -0.8380556477341514 1104 80-81 -0.8952941681575517 1104 82-83 -1.0250664078194056 1104 84-85 -0.8629833761969081 1104 86-87 -0.8074152020650587 1104 88-89 -0.9903278975184975 1104 90-91 -1.2495065093367437 1104 92-93 -1.1522199298304088 1104 94-95 -1.2745272598933006 1104 96-97 -1.8110314319594565 1104 98-99 -1.945301528966354 1104 100-101 -1.9482220409883269 1105 1 0.004813987949443543 1105 2 0.09261534961828133 1105 3 0.24347095938220775 1105 4 0.16981047221679546 1105 5 0.22788472825538975 1105 6 0.21559482311406697 1105 7 0.17929111115901009 1105 8 0.2773597208966052 1105 9 0.3913049470638441 1105 10-11 0.4275106595653355 1105 12-13 0.5359653901270178 1105 14-15 0.6359043751403632 1105 16-17 0.22922036453662287 1105 18-19 0.19965393556770294 1105 20-21 0.33317939502622096 1105 22-23 0.4889665174757525 1105 24-25 0.3429757285234487 1105 26-27 0.4722152544655387 1105 28-29 0.45297120876918484 1105 30-31 0.532266470770594 1105 32-33 0.5911146399479037 1105 34-35 0.46911936422804956 1105 36-37 0.6144900893361296 1105 38-39 0.8283491879228393 1105 40-41 1.016714778535821 1105 42-43 0.6456691555786662 1105 44-45 0.967967612929364 1105 46-47 1.2565705385427108 1105 48-49 1.0727672908929264 1105 50-51 1.4063535731145684 1105 52-53 1.3856518333272199 1105 54-55 1.4784371880029852 1105 56-57 0.9467146215905657 1105 58-59 0.8556218997795 1105 60-61 0.8939037044788165 1105 62-63 0.8915150530144658 1105 64-65 0.984708496185533 1105 66-67 0.9477045800738289 1105 68-69 0.8962329232089914 1105 70-71 0.7052365561834684 1105 72-73 0.7242300158728625 1105 74-75 0.8796566994146637 1105 76-77 0.7767577036044528 1105 78-79 0.643391165957766 1105 80-81 0.7184829805266766 1105 82-83 0.4789098185325109 1105 84-85 0.21484031526314595 1105 86-87 0.3445261095949874 1105 88-89 0.0766211002441608 1105 90-91 0.2548349971393655 1105 92-93 0.21536983015010946 1105 94-95 0.11420689227631087 1105 96-97 0.21321403628400049 1105 98-99 0.623816319533713 1105 100-101 0.4339522889509446 1106 1 0.2341360332360125 1106 2 0.25577689858914354 1106 3 0.2561924089107528 1106 4 0.274336889381793 1106 5 0.28513125113384064 1106 6 0.327153872588859 1106 7 0.4086355533637587 1106 8 0.32952214334727614 1106 9 0.4553377361520674 1106 10-11 0.27123616293607 1106 12-13 0.3115034760863864 1106 14-15 0.3811842246330741 1106 16-17 0.1549745807564733 1106 18-19 0.39931126271948614 1106 20-21 0.4108295103703483 1106 22-23 0.9998402212541109 1106 24-25 0.6532962284226613 1106 26-27 0.4674335124332387 1106 28-29 0.3218708481787971 1106 30-31 0.5215383469604333 1106 32-33 0.5327034773238779 1106 34-35 0.5370044232439284 1106 36-37 0.5972668592525991 1106 38-39 0.7102278414201209 1106 40-41 0.8770699936582176 1106 42-43 0.88780223806652 1106 44-45 0.8763082252611056 1106 46-47 0.944626262075154 1106 48-49 0.7958181990879538 1106 50-51 0.6389455684560126 1106 52-53 0.6262619253785537 1106 54-55 1.1122475756936439 1106 56-57 0.7394423422562042 1106 58-59 0.6552590697049183 1106 60-61 0.8881476965055128 1106 62-63 0.8086464557334523 1106 64-65 0.5499395203966024 1106 66-67 0.4081964163971499 1106 68-69 0.4862909312270851 1106 70-71 0.5868688435807208 1106 72-73 0.6639487105804704 1106 74-75 0.6538173749057172 1106 76-77 0.4525959082674902 1106 78-79 0.10870953814975337 1106 80-81 0.015566901779113351 1106 82-83 -0.18061223123344305 1106 84-85 -0.2051242976061225 1106 86-87 0.14131887091102868 1106 88-89 -0.1526113573696719 1106 90-91 -0.01906811379256723 1106 92-93 0.11213123578069428 1106 94-95 0.21130873106329418 1106 96-97 -0.2565389257084263 1106 98-99 -0.072324691463173 1106 100-101 -0.11324921612195737 1107 1 0.07947393974007966 1107 2 0.06975894465960408 1107 3 -0.15905572794561706 1107 4 -0.03202320546915871 1107 5 0.05844171861351555 1107 6 -0.09279446751953913 1107 7 -0.380088193248703 1107 8 0.07649453494619252 1107 9 0.2720298093227953 1107 10-11 0.2476187001989345 1107 12-13 0.2276514266283982 1107 14-15 0.17634428560867832 1107 16-17 -0.002259921276049681 1107 18-19 0.011131638735747629 1107 20-21 -0.05540101130986841 1107 22-23 0.15509513317823576 1107 24-25 0.1503427223250995 1107 26-27 -0.051028547187357276 1107 28-29 -0.039388467539353655 1107 30-31 0.11076810644552637 1107 32-33 0.08476563857049513 1107 34-35 0.09196716037129704 1107 36-37 0.07739685586506084 1107 38-39 0.0573759613388205 1107 40-41 -0.08446032476995669 1107 42-43 0.4418016452480913 1107 44-45 0.6642486046648983 1107 46-47 0.457414202454558 1107 48-49 0.6458668948874191 1107 50-51 0.6443550366131916 1107 52-53 0.5191099944842463 1107 54-55 0.5189739476448629 1107 56-57 0.3609277758605387 1107 58-59 0.15049535019272042 1107 60-61 0.1115237974540193 1107 62-63 -0.07990367976790225 1107 64-65 0.32323940183291455 1107 66-67 0.06482751395812514 1107 68-69 0.3462587496552736 1107 70-71 0.11572639778126259 1107 72-73 0.2656276570574363 1107 74-75 0.33559032544772194 1107 76-77 0.3017447903813135 1107 78-79 0.7643773057649312 1107 80-81 0.41492538755147024 1107 82-83 0.4417412579264486 1107 84-85 0.2750374571364276 1107 86-87 0.056916347198288975 1107 88-89 0.09080412366013846 1107 90-91 0.244429091491984 1107 92-93 -0.015314140235567208 1107 94-95 0.09117831100909513 1107 96-97 0.2075214570828976 1107 98-99 0.2746858098918423 1107 100-101 0.490695143634138 1108 1 0.11951217813670212 1108 2 0.13844301638956225 1108 3 0.3330131011683086 1108 4 0.1842610772312625 1108 5 0.38314504139832906 1108 6 0.21867045864079415 1108 7 0.11519793544327683 1108 8 0.3220575623723505 1108 9 0.10611472493461349 1108 10-11 0.2020721548402875 1108 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-1.5229332377272087 2316 100-101 -1.4510831301753804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3936.0 3 793.0 4 656.0 5 677.0 6 761.0 7 896.0 8 1004.0 9 1261.0 10 1589.0 11 2399.0 12 3512.0 13 5003.0 14 5305.0 15 6224.0 16 7785.0 17 9926.0 18 8137.0 19 4818.0 20 4469.0 21 4608.0 22 4606.0 23 4865.0 24 5088.0 25 5726.0 26 6374.0 27 7046.0 28 7982.0 29 8990.0 30 10197.0 31 12059.0 32 14029.0 33 17234.0 34 22692.0 35 36979.0 36 39091.0 37 53020.0 38 49766.0 39 8804.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.369624092731016 19.620997570298837 14.822466739918806 17.186911597051342 2 15.124412988231411 24.17766803614601 39.93278038671897 20.76513858890361 3 20.428450295487405 31.569601265969595 31.121151125120168 16.880797313422836 4 11.501431911389044 19.476314819445125 38.07131460034077 30.950938668825064 5 10.943494492801452 41.48782022193804 32.282857555381376 15.28582772987913 6 24.476978285678953 37.74540995473714 20.006594929632616 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29.399804667870953 37.19157237209998 16.509688009751144 30-31 17.037794772427443 30.760816911279683 36.06298441622691 16.138403900065963 32-33 16.46169205500628 30.801970951016074 35.497858362049534 17.238478631928118 34-35 17.084587483165016 29.7617986929725 35.69145783538581 17.46215598847667 36-37 17.898260450040162 29.721353819727835 35.7242175000097 16.656168230222303 38-39 18.502239102238327 29.092423260800125 35.864947801696886 16.540389835264662 40-41 17.038039719139835 29.85503199173606 36.554744072575794 16.552184216548305 42-43 16.37484982392704 31.2679192870694 35.07556976381782 17.28166112518574 44-45 16.340398454192254 31.332483173561926 34.01423715836252 18.312881213883298 46-47 16.890349498766803 30.568952383169535 32.82660344829816 19.714094669765505 48-49 17.430325138573583 30.81423702065183 32.727037532905165 19.02840030786943 50-51 17.75550194985352 31.36981251657214 32.64488090695674 18.2298046266176 52-53 16.564544148101582 31.324772892195096 33.090800436171165 19.019882523532157 54-55 15.936701738956108 31.06439117135411 33.15540667257312 19.84350041711666 56-57 16.946044677205574 31.473054666537355 31.31930300973071 20.261597646526365 58-59 17.10529037596318 30.203883746186065 31.697522883591045 20.99330299425971 60-61 17.189947831302252 29.313741203964618 32.8347405266505 20.661570438082634 62-63 18.619839659402324 28.866610021093365 32.552193464032 19.96135685547231 64-65 17.79929288211985 29.50878205219425 33.26985745093448 19.422067614751416 66-67 17.07910257189761 29.850105238102014 32.89677093022203 20.174021259778346 68-69 16.838250991402454 30.418103364622624 32.80898440279728 19.93466124117765 70-71 17.29446073192758 29.821533461505616 31.950349196163113 20.93365661040369 72-73 17.118902577065526 29.278237955216678 31.858149495932143 21.744709971785653 74-75 17.287467304154795 29.485895833818436 32.49326679033523 20.733370071691542 76-77 17.883143236640315 28.387499789835267 32.84544938392634 20.883907589598074 78-79 17.141800578229006 28.526912474333976 33.56081489948228 20.770472047954737 80-81 17.31180779600308 29.488649955711878 32.59476688929262 20.604775358992427 82-83 17.263297291075823 28.988217401931305 32.24469420983137 21.503791097161496 84-85 17.714069196330424 28.00312436148449 32.33649476643588 21.946311675749214 86-87 18.24744921384441 27.903641134878356 32.3705724185294 21.478337232747837 88-89 18.82502984702028 27.03784330540713 32.52736677493083 21.609760072641762 90-91 18.29548614928887 27.157354168148863 33.044698699333175 21.502460983229092 92-93 18.47390908961551 27.801285044764416 32.31808673987481 21.406719125745266 94-95 18.61270195692362 27.5997769725446 31.740920751514558 22.04660031901723 96-97 18.879084286662362 26.558972720322178 31.42240615374266 23.1395368392728 98-99 19.497609512278412 26.40156571771548 31.487431829706207 22.6133929402999 100-101 20.638250540627233 25.679276128591678 32.17262305089656 21.509850279884528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4903.0 1 3116.5 2 1840.5 3 2205.5 4 1712.0 5 1515.0 6 2371.0 7 4018.0 8 5364.0 9 5278.0 10 3806.0 11 2605.0 12 2324.5 13 2205.0 14 2091.0 15 1907.5 16 1838.5 17 1841.0 18 1775.0 19 1768.0 20 1881.5 21 2097.0 22 2274.5 23 2496.5 24 2862.5 25 3318.5 26 3880.5 27 4473.0 28 4976.0 29 5497.5 30 6221.0 31 6906.5 32 7502.5 33 8342.5 34 9367.0 35 10187.0 36 10838.5 37 11328.0 38 11068.0 39 10221.0 40 9684.5 41 9425.0 42 9205.0 43 9134.0 44 9428.5 45 10054.0 46 11753.0 47 15226.5 48 21253.0 49 20633.5 50 14197.0 51 10838.0 52 8487.0 53 7200.0 54 6327.0 55 5536.5 56 4897.5 57 4310.0 58 3805.0 59 3448.5 60 3093.5 61 2717.5 62 2250.5 63 1638.0 64 1152.0 65 887.5 66 710.0 67 584.0 68 473.5 69 382.0 70 328.5 71 265.0 72 216.5 73 206.5 74 186.5 75 153.5 76 114.5 77 79.0 78 67.0 79 49.5 80 36.0 81 27.5 82 18.5 83 16.0 84 14.0 85 9.5 86 7.0 87 4.5 88 1.5 89 0.5 90 1.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15709017491089639 2 0.08498320937802592 3 0.07957518696306062 4 0.09502667957724717 5 0.11743134386781762 6 0.034765858381919694 7 0.038628731535466324 8 0.03991635591998187 9 0.04146150518140052 10-11 0.03991635591998187 12-13 0.043779229073528506 14-15 0.042105317373658295 16-17 0.04004511835843342 18-19 0.044165516388883164 20-21 0.04545314077339871 22-23 0.059101959249263473 24-25 0.07699993819402955 26-27 0.07751498794783576 28-29 0.06566884361029275 30-31 0.05562537341107151 32-33 0.044938091019592494 34-35 0.28263355240116195 36-37 0.4497671975112796 38-39 0.4278775829745153 40-41 0.4047003440532356 42-43 0.43328560538948063 44-45 0.44242773851954104 46-47 0.4417839263272832 48-49 0.45955314283359766 50-51 0.44925214775747335 52-53 0.45491769504934176 54-55 0.4453892746039268 56-57 0.424787284451678 58-59 0.40482910649168713 60-61 0.38371206658563217 62-63 0.43714847854302724 64-65 0.4670213642637879 66-67 0.4654762150023692 68-69 0.48015513298584644 70-71 0.4771935969014607 72-73 0.46521869012546613 74-75 0.46225715404108036 76-77 0.4404963019427677 78-79 0.4156451513216176 80-81 0.422212035682647 82-83 0.43328560538948063 84-85 0.43083911905890104 86-87 0.4417839263272832 88-89 0.45259997115721384 90-91 0.45826551844908214 92-93 0.4605832423412101 94-95 0.4667638393868848 96-97 0.46753641401759405 98-99 0.4583942808875337 100-101 0.4439728877809596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 388312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.957237627909855 #Duplication Level Percentage of deduplicated Percentage of total 1 89.4657734206792 56.325179568104424 2 6.841159222029488 8.614009735833546 3 1.4596207028668575 2.7568106231101663 4 0.6256715140913887 1.5756220075866283 5 0.3654291563616924 1.1503205116614847 6 0.2323358224822638 0.8776332951295058 7 0.17042507587441716 0.7510644399706173 8 0.11478757831296375 0.5781367075665256 9 0.08678287783634428 0.4917249235778938 >10 0.5438974419114544 6.3782742657334985 >50 0.04132972149113275 1.80362749348226 >100 0.042148131817550755 5.385892926531232 >500 0.0053196671226034935 2.369112759608783 >1k 0.004092051632771918 5.710241878722125 >5k 0.0012276154898315755 5.2323488633812945 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8179 2.106295968190527 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6157 1.585580667092441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 1.5384536146191723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 1.209594346813902 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2898 0.7463070932652094 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.6958322173922001 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2584 0.6654442819176333 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2127 0.5477554131729125 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1954 0.5032036094686746 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1753 0.45144110921114977 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1347 0.3468860091884876 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1078 0.2776118173015513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1025 0.2639629988256866 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 991 0.25520715301098085 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 822 0.21168544881435547 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 798 0.20550485176868086 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 797 0.20524732689177774 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 739 0.19031088403139743 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 736 0.1895383094006881 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 716 0.18438781186262593 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.17975236407836997 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.15168215249593112 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 583 0.15013700323451246 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 578 0.1488493788499969 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 575 0.1480768042192876 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 574 0.14781927934238448 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 453 0.1166587692371083 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 440 0.11331094583736788 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 429 0.11047817219143369 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 421 0.10841797317620883 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 416 0.10713034879169328 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 406 0.10455510002266219 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.5752487690310885E-4 0.0 10-11 0.0 0.0 0.0 2.5752487690310885E-4 0.0 12-13 0.0 0.0 0.0 2.5752487690310885E-4 0.0 14-15 0.0 0.0 0.0 2.5752487690310885E-4 0.0 16-17 0.0 0.0 0.0 7.725746307093266E-4 0.0 18-19 0.0 0.0 0.0 0.0010300995076124354 0.0 20-21 0.0 0.0 0.0 0.001931436576773316 0.0 22-23 0.0 0.0 0.0 0.0075969838686417105 0.0 24-25 0.0 0.0 0.0 0.014936442860380313 0.0 26-27 0.0 0.0 0.0 0.03180432229753394 0.0 28-29 0.0 0.0 0.0 0.07622736356332022 0.0 30-31 0.0 0.0 0.0 0.18580419868559303 0.0 32-33 0.0 0.0 0.0 0.3367137765508148 0.0 34-35 0.0 0.0 0.0 0.5919209295617957 0.0 36-37 0.0 0.0 0.0 0.9529708069799543 0.0 38-39 0.0 0.0 0.0 1.3821360143389851 0.0 40-41 0.0 0.0 0.0 1.8731071921547622 0.0 42-43 0.0 0.0 0.0 2.266218916747358 0.0 44-45 0.0 0.0 0.0 2.663579801808855 0.0 46-47 0.0 0.0 0.0 3.1372968128721235 0.0 48-49 0.0 0.0 0.0 3.655179340324275 0.0 50-51 0.0 0.0 0.0 4.177697315560684 0.0 52-53 0.0 0.0 0.0 4.647551453470405 0.0 54-55 0.0 0.0 0.0 5.14251426687818 0.0 56-57 0.0 0.0 0.0 5.685247944951483 0.0 58-59 0.0 0.0 0.0 6.320433053833 0.0 60-61 0.0 0.0 0.0 7.050902367168669 0.0 62-63 0.0 0.0 0.0 7.710938626671336 0.0 64-65 0.0 0.0 0.0 8.321530109808606 0.0 66-67 0.0 0.0 0.0 9.004357320917201 0.0 68-69 0.0 0.0 0.0 9.730706236222419 0.0 70-71 0.0 0.0 0.0 10.53920558725973 0.0 72-73 0.0 0.0 0.0 11.352340386081295 0.0 74-75 0.0 0.0 0.0 12.132383238220811 0.0 76-77 0.0 0.0 0.0 12.893111724592597 0.0 78-79 0.0 0.0 0.0 13.730453861843054 0.0 80-81 0.0 0.0 0.0 14.633979892457612 0.0 82-83 0.0 0.0 0.0 15.553987515193967 0.0 84-85 0.0 0.0 0.0 16.598250891036074 0.0 86-87 0.0 0.0 0.0 17.627320299140898 0.0 88-89 0.0 0.0 0.0 18.541919899462286 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 60 0.0039853826 31.60535 1 GCGGTAT 75 3.1098648E-4 31.605349 1 CACTACA 80 4.560061E-4 29.607098 2 GATCAGT 65 0.005935917 29.13283 6 CATGGGT 295 0.0 27.29875 4 CCGTATC 70 5.7465877E-7 27.172672 94-95 GTACTAC 70 0.008496486 27.069347 3 CGGTATC 70 0.008496486 27.069347 2 GTATTAT 135 1.3600147E-5 24.58194 1 AGTACTT 4930 0.0 22.906641 12-13 GAGTACT 4920 0.0 22.760721 12-13 ATGGGAG 735 0.0 21.91328 5 ATGGGGA 500 0.0 21.790825 5 CTATAAG 110 0.002919548 21.532436 2 GTCACTA 110 0.002919548 21.532436 2 ACTGTCC 110 0.002919548 21.532436 3 CATGGGG 1205 0.0 21.228659 4 CGCCGTA 115 8.9070454E-8 20.674858 94-95 TACATGG 5030 0.0 20.624905 2 ATGGGTG 230 9.242831E-8 20.596243 5 >>END_MODULE