##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938809_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388312 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.826309771523928 31.0 31.0 34.0 30.0 34.0 2 31.98380683574033 33.0 31.0 34.0 30.0 34.0 3 32.01772286202847 33.0 31.0 34.0 30.0 34.0 4 35.72045674612168 37.0 35.0 37.0 33.0 37.0 5 35.64491697397968 37.0 35.0 37.0 33.0 37.0 6 35.731301118688066 37.0 35.0 37.0 33.0 37.0 7 35.65581285152146 37.0 35.0 37.0 33.0 37.0 8 35.66843157048971 37.0 35.0 37.0 33.0 37.0 9 37.22023269947877 39.0 37.0 39.0 33.0 39.0 10-11 37.155018387276215 39.0 37.0 39.0 33.0 39.0 12-13 37.07562861822452 39.0 37.0 39.0 33.0 39.0 14-15 38.05999170769897 40.0 37.0 41.0 33.0 41.0 16-17 38.02901790312944 40.0 37.0 41.0 33.0 41.0 18-19 38.15242897463895 40.0 37.0 41.0 33.0 41.0 20-21 38.22262253033644 40.0 37.0 41.0 33.0 41.0 22-23 38.096507447619445 40.0 37.0 41.0 33.0 41.0 24-25 37.948189857640244 40.0 37.0 41.0 33.0 41.0 26-27 37.61133186715837 40.0 36.0 41.0 32.5 41.0 28-29 37.319526566266305 39.0 35.5 41.0 32.0 41.0 30-31 36.89729650384227 39.0 35.0 41.0 31.5 41.0 32-33 36.40388914069099 38.0 35.0 41.0 31.0 41.0 34-35 36.071530109808606 38.0 35.0 41.0 30.0 41.0 36-37 35.7505240631245 38.0 35.0 41.0 30.0 41.0 38-39 35.42376104781722 38.0 35.0 40.5 28.5 41.0 40-41 35.05418065885165 38.0 35.0 40.0 26.5 41.0 42-43 34.68785538433013 38.0 35.0 40.0 24.5 41.0 44-45 34.15339598055172 38.0 34.0 40.0 22.0 41.0 46-47 33.43553894806239 38.0 33.0 40.0 13.5 41.0 48-49 32.83911133315478 38.0 33.0 40.0 2.0 41.0 50-51 32.43786697294959 37.0 33.0 40.0 2.0 41.0 52-53 32.127409145223425 37.0 33.0 40.0 2.0 41.0 54-55 31.835786686993963 37.0 32.0 40.0 2.0 41.0 56-57 31.497928212365316 36.0 31.5 40.0 2.0 41.0 58-59 31.12154144090319 36.0 31.0 40.0 2.0 41.0 60-61 30.67178840726014 35.5 30.5 39.5 2.0 41.0 62-63 30.182171810296875 35.0 29.5 39.0 2.0 41.0 64-65 29.695345495374852 35.0 28.5 39.0 2.0 40.0 66-67 29.197873102041655 35.0 28.0 38.5 2.0 40.0 68-69 28.709872473680957 35.0 26.5 38.0 2.0 40.0 70-71 28.201792630668123 35.0 26.0 37.0 2.0 39.5 72-73 27.856451770741053 34.0 26.0 36.5 2.0 39.0 74-75 27.46472295473743 34.0 25.5 36.0 2.0 39.0 76-77 25.54425307484703 31.5 21.0 34.5 2.0 36.5 78-79 26.447910443148807 33.5 23.0 35.0 2.0 37.0 80-81 26.45626068728239 34.0 23.0 35.0 2.0 37.0 82-83 26.131183172294442 34.0 20.5 35.0 2.0 36.5 84-85 25.740494756793506 33.5 19.0 35.0 2.0 36.0 86-87 25.199463318156532 33.0 9.5 35.0 2.0 35.5 88-89 24.663806423700528 33.0 2.0 35.0 2.0 35.0 90-91 24.217420527822988 32.5 2.0 35.0 2.0 35.0 92-93 23.94080790704382 32.0 2.0 35.0 2.0 35.0 94-95 23.594345526277838 32.0 2.0 35.0 2.0 35.0 96-97 23.100652310513198 32.0 2.0 35.0 2.0 35.0 98-99 22.46655653186098 31.0 2.0 34.0 2.0 35.0 100-101 19.999063897072457 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07946973991736073 1101 2 0.17405871895190472 1101 3 0.2642757446850581 1101 4 0.3036857796827377 1101 5 0.253925789911122 1101 6 0.2873069003759454 1101 7 0.31652493240748925 1101 8 0.2616102573773631 1101 9 0.5846385989004688 1101 10-11 0.34263737582178777 1101 12-13 0.4194849228664239 1101 14-15 0.5165308047714134 1101 16-17 0.49615457433565524 1101 18-19 0.6651454917230311 1101 20-21 0.569034778832787 1101 22-23 0.5306997702112284 1101 24-25 0.6796588826472814 1101 26-27 0.7512583459073383 1101 28-29 0.6781926574112731 1101 30-31 0.6975634848886969 1101 32-33 0.6166094383829943 1101 34-35 0.5364251027811164 1101 36-37 0.09159380997648014 1101 38-39 0.20362762306818638 1101 40-41 0.44952930196269136 1101 42-43 0.8573638124175105 1101 44-45 0.5235954821271918 1101 46-47 0.49535860740223114 1101 48-49 0.1426705795396046 1101 50-51 -0.06839597441111778 1101 52-53 -0.18982957484674756 1101 54-55 -0.5275813173998181 1101 56-57 -0.31588755940779123 1101 58-59 -0.20853021942417982 1101 60-61 -0.3972492923009021 1101 62-63 -0.6244197987390443 1101 64-65 -0.77499982036014 1101 66-67 -0.9822071496514688 1101 68-69 -0.6592183632782458 1101 70-71 -0.5102150134583319 1101 72-73 -0.12121659763290182 1101 74-75 -0.3113495140312317 1101 76-77 -0.6613282311167126 1101 78-79 -0.49712779347589375 1101 80-81 -0.19130052194960712 1101 82-83 -0.02925854363870073 1101 84-85 -0.3249431465532986 1101 86-87 -0.7667299579794502 1101 88-89 -0.26715009208607654 1101 90-91 -0.25279142640674834 1101 92-93 -0.3143704214364611 1101 94-95 -0.35319978256283235 1101 96-97 -0.49264510347355994 1101 98-99 -0.5220856731973882 1101 100-101 -0.3454665454274348 1102 1 0.04792375104477742 1102 2 0.04286821099870508 1102 3 -0.06062439891981697 1102 4 -0.08204633052419297 1102 5 0.025578344586911328 1102 6 -0.10415371029790066 1102 7 0.12834761569988729 1102 8 0.15728877079225612 1102 9 0.09854002160886921 1102 10-11 0.09144263034769295 1102 12-13 0.07590045102632104 1102 14-15 -0.019127148819883644 1102 16-17 0.2992628743835226 1102 18-19 0.08414214094261752 1102 20-21 0.11236735359756267 1102 22-23 -0.004232311745560935 1102 24-25 0.022748977049829477 1102 26-27 -0.06900799012807113 1102 28-29 -0.18072143862350742 1102 30-31 -0.5881905976487971 1102 32-33 0.040181040715197014 1102 34-35 0.15123052994676556 1102 36-37 -0.22232016834939117 1102 38-39 -0.13097958228678408 1102 40-41 -0.4752076186151797 1102 42-43 -0.2205124048888436 1102 44-45 -0.8393089460821415 1102 46-47 -0.5976353580541272 1102 48-49 -0.73321321822079 1102 50-51 -0.5830114978626568 1102 52-53 -0.8043525006195082 1102 54-55 -0.5493456955436322 1102 56-57 -0.6096301535553046 1102 58-59 -0.2661094963775632 1102 60-61 -0.5398614885138109 1102 62-63 -0.8232654666586576 1102 64-65 -0.766854363531845 1102 66-67 -0.5119757339271302 1102 68-69 -0.7513569772950213 1102 70-71 -0.45745003107543525 1102 72-73 -0.24129538413247786 1102 74-75 -0.3012697073877426 1102 76-77 -0.40894854924466983 1102 78-79 -0.44145147729405565 1102 80-81 -0.2633423007667126 1102 82-83 -0.37825762550917474 1102 84-85 -0.06724302178185226 1102 86-87 0.09566832190095553 1102 88-89 0.05246884492396475 1102 90-91 0.020336413320794833 1102 92-93 0.22696110179535722 1102 94-95 -0.04146341914048435 1102 96-97 -0.24251758548371427 1102 98-99 -0.07730684824623779 1102 100-101 0.2693074926945549 1103 1 -0.01206468817479589 1103 2 -0.0013330066367416293 1103 3 -0.10043402139277546 1103 4 0.0042316236680903785 1103 5 -0.03297625757961242 1103 6 0.02813464859676884 1103 7 0.10009126665440249 1103 8 0.03581049229756417 1103 9 0.04544300864427697 1103 10-11 -0.1312450258917508 1103 12-13 0.012796606691473755 1103 14-15 -0.1806102317947591 1103 16-17 -0.41754680063844063 1103 18-19 -0.15324046834054172 1103 20-21 -0.3719242483212213 1103 22-23 -0.6446957939427094 1103 24-25 -0.5338613120447491 1103 26-27 -0.5799944850345753 1103 28-29 -0.549818121280083 1103 30-31 -0.45911523487300343 1103 32-33 -0.6968900280564299 1103 34-35 -1.1955816223852906 1103 36-37 -1.3627311015445898 1103 38-39 -1.1014845781954676 1103 40-41 -1.1227201740731445 1103 42-43 -0.9128980545071101 1103 44-45 -1.2596484126347036 1103 46-47 -0.9223694225692896 1103 48-49 -1.060278206322188 1103 50-51 -1.0387277032241542 1103 52-53 -1.3529340529170284 1103 54-55 -1.3926595857462445 1103 56-57 -1.2063540986340904 1103 58-59 -1.155880457799089 1103 60-61 -0.9594877900399865 1103 62-63 -1.1327302926088585 1103 64-65 -1.348492991552476 1103 66-67 -1.479477929724709 1103 68-69 -1.2351643776958952 1103 70-71 -1.4123930154083233 1103 72-73 -1.2247311880196037 1103 74-75 -1.103280082062124 1103 76-77 -1.1864823495134225 1103 78-79 -1.1646120745509982 1103 80-81 -0.8498754929684402 1103 82-83 -1.021067045293119 1103 84-85 -0.8925326043175694 1103 86-87 -0.9031756000562687 1103 88-89 -0.8545081611341843 1103 90-91 -0.9270507752325301 1103 92-93 -0.7660198529449396 1103 94-95 -1.1646532936985103 1103 96-97 -1.2200378731519663 1103 98-99 -0.8591308991737279 1103 100-101 -0.629025202543751 1104 1 -0.36061759359335 1104 2 -0.026299692767690175 1104 3 -0.1295780421147512 1104 4 -0.031343096720000574 1104 5 0.03192964009814858 1104 6 0.012840213884466323 1104 7 -0.1832637768407821 1104 8 -0.201421686654065 1104 9 -0.44102931464249195 1104 10-11 -0.040522751265690715 1104 12-13 -0.1455378495812667 1104 14-15 -0.3991641852900898 1104 16-17 0.03993871344322031 1104 18-19 0.011595807094032295 1104 20-21 -0.12061040921598476 1104 22-23 -0.012806560685739043 1104 24-25 -0.10014093892941389 1104 26-27 -0.17002802349149704 1104 28-29 -0.29715675488741056 1104 30-31 -0.41359501447743696 1104 32-33 -0.436158470464612 1104 34-35 -0.4597755764795224 1104 36-37 -0.44186838463013345 1104 38-39 -0.47563301581244133 1104 40-41 -0.22397562032926288 1104 42-43 -0.37917618413923293 1104 44-45 -0.01959391264218624 1104 46-47 -0.16749960867777958 1104 48-49 -0.657483584453324 1104 50-51 -0.6557529618745335 1104 52-53 -0.36182024675133917 1104 54-55 -0.3663977761668207 1104 56-57 -0.10382598771762375 1104 58-59 -0.19249951183493152 1104 60-61 -0.11888155248589882 1104 62-63 -0.41397421002040957 1104 64-65 0.1547186151492248 1104 66-67 -0.3106664487922224 1104 68-69 -0.5838932102880889 1104 70-71 -0.6319576143692309 1104 72-73 -0.5922580262714092 1104 74-75 -0.7974512779177267 1104 76-77 -0.6839519927181392 1104 78-79 -0.5903970091959252 1104 80-81 -0.39487916123716715 1104 82-83 -0.3565713948117555 1104 84-85 -0.3574819223190353 1104 86-87 -0.2874716667738433 1104 88-89 -0.1965555042595497 1104 90-91 -0.05876782444722295 1104 92-93 -0.1295340543852994 1104 94-95 -0.10066820633382534 1104 96-97 0.2539585933320332 1104 98-99 -0.10302959506676501 1104 100-101 0.07128098560100327 1105 1 -0.1911504459745217 1105 2 0.001332226627159372 1105 3 0.11865263228367695 1105 4 -0.06356384767505574 1105 5 -0.022360118360367665 1105 6 0.060319020027257864 1105 7 -0.06616105702585884 1105 8 0.0479048281169554 1105 9 0.2703080672122198 1105 10-11 0.04724721357609951 1105 12-13 -0.10955775734803552 1105 14-15 0.14300283904669442 1105 16-17 0.2503628949874397 1105 18-19 0.20942162935481434 1105 20-21 0.13116652638817072 1105 22-23 0.1356912248035016 1105 24-25 0.017315646005819474 1105 26-27 0.036671061786186954 1105 28-29 -0.14573292340331534 1105 30-31 -0.498914558486085 1105 32-33 -0.4814988658803401 1105 34-35 -0.36631332970235775 1105 36-37 -0.33927032974678895 1105 38-39 -0.3445583312470504 1105 40-41 -0.41903140164823327 1105 42-43 -1.0934396119887992 1105 44-45 -0.8999875019760069 1105 46-47 -0.7493756265156293 1105 48-49 -0.8824466759407699 1105 50-51 -0.6110137566632012 1105 52-53 -0.6269603468989757 1105 54-55 -0.2698572833430539 1105 56-57 -0.39281964097546407 1105 58-59 -0.4898185079233919 1105 60-61 -0.503251368171636 1105 62-63 -0.16671761595316426 1105 64-65 -0.06377216913763917 1105 66-67 -0.13801451509056406 1105 68-69 0.023419241198144647 1105 70-71 0.1614384253854233 1105 72-73 0.214360392978854 1105 74-75 0.14849487370396375 1105 76-77 0.20233934862091374 1105 78-79 -0.11317755194130896 1105 80-81 0.7305070463941448 1105 82-83 0.26692372717658586 1105 84-85 0.3029141022156132 1105 86-87 0.25023209104543653 1105 88-89 0.5654340548168975 1105 90-91 0.3308833330341727 1105 92-93 0.0779184211485422 1105 94-95 0.4079463165951154 1105 96-97 0.5755230576639541 1105 98-99 0.9264857999684715 1105 100-101 0.9776513187669131 1106 1 -0.03645493207723405 1106 2 0.033897345666780154 1106 3 -0.0760437774903373 1106 4 0.04217200307188307 1106 5 0.12962536843664907 1106 6 -0.028775920508920194 1106 7 0.1488717544592788 1106 8 0.09543108850352411 1106 9 -0.09005834636926835 1106 10-11 0.048971776276275136 1106 12-13 -0.04004892176380537 1106 14-15 0.13781795248708306 1106 16-17 -0.1600942261126974 1106 18-19 -0.11123504828633912 1106 20-21 -0.21609769005564772 1106 22-23 -0.2341266882516564 1106 24-25 -0.30757404917212483 1106 26-27 -0.16129529804270248 1106 28-29 -0.167704300141871 1106 30-31 -0.1894675383960447 1106 32-33 -0.14946455380169255 1106 34-35 -0.04246509121997377 1106 36-37 -0.1662541367749455 1106 38-39 -0.05405910394072322 1106 40-41 -0.046839415265559126 1106 42-43 0.004446118934630761 1106 44-45 0.04116459549538121 1106 46-47 0.32966309775591185 1106 48-49 0.12264862294610168 1106 50-51 0.07780346208749478 1106 52-53 0.026469741120912715 1106 54-55 -0.128431943469824 1106 56-57 -0.16434867857988777 1106 58-59 -0.10709996999420923 1106 60-61 0.02018700670798168 1106 62-63 0.26293366403070806 1106 64-65 0.13524684584589863 1106 66-67 0.26442450929967976 1106 68-69 0.008738061328497082 1106 70-71 -0.042474316221330355 1106 72-73 0.055756616858445796 1106 74-75 -0.08430989236022768 1106 76-77 -0.24745795926951786 1106 78-79 -0.030465733087584823 1106 80-81 -0.208954083754346 1106 82-83 -0.29748980952076565 1106 84-85 -0.5253239322308474 1106 86-87 -0.32864985479881526 1106 88-89 -0.07944041045668015 1106 90-91 -0.044936954094321635 1106 92-93 -0.14948868763328704 1106 94-95 -0.13348798204539492 1106 96-97 -0.12383191109234915 1106 98-99 -0.4512718205829387 1106 100-101 -0.22252113750310087 1107 1 -0.2659180079579926 1107 2 -0.08445461910286767 1107 3 -0.3786140688182478 1107 4 -0.14674652673841848 1107 5 -0.48254434701050286 1107 6 -0.22110315628128063 1107 7 -0.18539315068976947 1107 8 -0.1307926581089447 1107 9 -0.2984763680494851 1107 10-11 -0.15137968036329852 1107 12-13 -0.6121081189182789 1107 14-15 -0.3203989343692797 1107 16-17 -0.4771250526709636 1107 18-19 -0.5474223789096513 1107 20-21 -0.5367807015732566 1107 22-23 -0.48180834475572 1107 24-25 -0.2928276907710341 1107 26-27 -0.36352048435706763 1107 28-29 -0.6271809261581325 1107 30-31 -0.7947457396155571 1107 32-33 -0.7879511730428845 1107 34-35 -1.0746466630357006 1107 36-37 -1.1636182119781964 1107 38-39 -1.2783350185748716 1107 40-41 -0.9992763224471304 1107 42-43 -0.8208629935314988 1107 44-45 -0.5464772574856482 1107 46-47 -0.9978690838158997 1107 48-49 -0.9284607943980703 1107 50-51 -0.6732339701618244 1107 52-53 -0.6805932670091686 1107 54-55 -0.44199054780586167 1107 56-57 -0.4538346203143071 1107 58-59 -0.5789305086120926 1107 60-61 -0.11387464640855072 1107 62-63 0.0719402697217646 1107 64-65 -0.10925899765003422 1107 66-67 -0.43488951509056406 1107 68-69 0.10620376254800945 1107 70-71 -0.1682576834300029 1107 72-73 -0.35878225509276973 1107 74-75 -0.09868573924369528 1107 76-77 -0.3391013366407911 1107 78-79 -0.4743173591038463 1107 80-81 -0.3705712077380845 1107 82-83 -0.0747171130437998 1107 84-85 -0.1493500162416872 1107 86-87 -0.3835596617093877 1107 88-89 0.02544610715849771 1107 90-91 0.3025710912986348 1107 92-93 -0.09772293560076761 1107 94-95 -0.3538047164627365 1107 96-97 -0.3631929375151124 1107 98-99 -0.5139411972767078 1107 100-101 -0.1910276585058135 1108 1 0.024311422421753548 1108 2 -0.00350788914505884 1108 3 0.07435929616065096 1108 4 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64-65 0.18184963330234538 2316 66-67 0.27604798490943594 2316 68-69 0.06583917921467375 2316 70-71 0.013661959427139436 2316 72-73 -0.10785219556896308 2316 74-75 -4.714535294105815E-4 2316 76-77 -0.08296294378364877 2316 78-79 -0.01710009719908001 2316 80-81 0.2821445660714339 2316 82-83 -0.19748497018665745 2316 84-85 0.0660517694725975 2316 86-87 -0.436945078376052 2316 88-89 -0.5195687737938854 2316 90-91 -0.4948619444156499 2316 92-93 -0.9995086498864829 2316 94-95 -0.978060668843689 2316 96-97 -1.3953729970389226 2316 98-99 -1.6746554829909925 2316 100-101 -2.2979845037439084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 54.0 6 154.0 7 320.0 8 546.0 9 908.0 10 1465.0 11 2287.0 12 3246.0 13 4457.0 14 4757.0 15 5960.0 16 7397.0 17 9567.0 18 8608.0 19 5420.0 20 4611.0 21 4630.0 22 4829.0 23 5099.0 24 5462.0 25 6047.0 26 6310.0 27 6605.0 28 7625.0 29 8887.0 30 10426.0 31 12141.0 32 14314.0 33 17295.0 34 23278.0 35 36463.0 36 40264.0 37 59519.0 38 53566.0 39 5764.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.386577646125254 20.581735280205336 15.48269863548363 17.54898843818578 2 15.230021220049858 24.966779290879497 39.14146356538041 20.661735923690227 3 20.184284801911865 31.27819897402089 31.927934238447435 16.609581985619812 4 11.664846824203218 20.671264344135643 36.77146212324111 30.892426708420036 5 10.618265724468984 42.381126516821524 32.403067636333674 14.597540122375822 6 24.31343867817631 38.45747749232576 19.258997919198993 17.970085910298934 7 22.472908382949793 39.462854611755496 20.983642019819115 17.080594985475596 8 24.66470261027215 33.70846123735553 20.78869568800346 20.838140464368855 9 26.315952120974885 18.92395805434805 22.127309998145822 32.63277982653124 10-11 22.714080430169552 29.258045077154453 26.553261295041104 21.47461319763489 12-13 27.869857228208243 27.92007457920435 27.029682317311853 17.180385875275554 14-15 23.46328072086549 28.86565150098761 29.80871002881711 17.862357749329792 16-17 17.332917851840502 32.166508500416555 31.203861917644527 19.296711730098416 18-19 16.950331049081793 29.979191936360287 35.82566551037699 17.244811504180927 20-21 19.016538247594717 28.286661241475926 34.71589855580049 17.980901955128868 22-23 17.84956426790828 29.45724056943901 34.2651012587816 18.428093903871115 24-25 18.25207565050784 29.763566410515256 35.395120418632445 16.589237520344465 26-27 18.590639976719693 28.67366621084646 36.14718614718615 16.5885076652477 28-29 16.44953027462453 29.350238983085763 37.06619934485671 17.13403139743299 30-31 16.643575269371023 30.84246688230083 36.05168009229692 16.46227775603123 32-33 16.14886998084015 31.020159047363975 35.54654504625147 17.284425925544408 34-35 16.80160092511632 30.272319525697604 35.62122771073632 17.30485183844976 36-37 17.74193132936661 30.081266259691418 35.31708008139505 16.859722329546916 38-39 18.42584114695008 29.160276048930562 35.638462955021645 16.775419849097716 40-41 16.86088214046099 29.933306977205383 36.57724041038294 16.628570471950685 42-43 16.486779517845783 31.358180165285 35.022624317858146 17.132415999011073 44-45 16.179591248094276 31.642604763278253 33.85684927273476 18.320954715892704 46-47 17.008002781336714 30.596635355167685 32.33612969270092 20.059232170794676 48-49 17.468041162776323 30.759930159253386 32.38349574568903 19.388532932281258 50-51 17.634106770363882 31.426437640029874 32.42139012644537 18.518065463160877 52-53 16.467299491130845 31.62057829786357 33.01996847895507 18.892153732050517 54-55 15.779708069799542 31.22270236304827 32.73372957827726 20.263859988874923 56-57 17.025742186695233 31.82492428768619 30.564340015245474 20.5849935103731 58-59 16.655485751737334 31.052469608728067 31.357380240812443 20.934664398722155 60-61 17.077357108183033 30.58179107129751 31.618979190568346 20.721872629951108 62-63 18.0873884917283 30.464291600568615 31.340004944477634 20.10831496322545 64-65 17.31764663466491 30.9100671624879 31.87076371577494 19.901522487072253 66-67 16.415877128148605 30.985277113211968 31.844777457012107 20.75406830162732 68-69 16.273338312173262 31.20213231696325 31.63876799464346 20.88576137622003 70-71 16.712635685865493 30.53244228119648 30.721984007416854 22.032938025521176 72-73 16.225483631718824 29.822024557572263 30.64481653927769 23.30767527143122 74-75 16.110498774181586 29.899797070397 31.278456498897793 22.71124765652362 76-77 16.34394549827169 28.89385515181041 32.199169482743955 22.56302986717395 78-79 15.582562101100656 28.7472870014362 32.708619123983226 22.961531773479916 80-81 15.582057726776407 29.7134778219576 31.73891097880055 22.96555347246544 82-83 15.332902408372648 29.232163827025694 31.348245740538538 24.086688024063125 84-85 15.619141309050455 27.94363810544099 31.45473227713797 24.982488308370588 86-87 15.757954029629017 28.022608632856024 31.52029257623901 24.699144761275946 88-89 16.227576701341746 27.165534871644496 31.64612556659721 24.96076286041654 90-91 15.33226338330594 27.236597972087473 32.07048242006686 25.360656224539728 92-93 15.00236922886751 27.68546941634562 31.437478110385463 25.87468324440141 94-95 14.79287274150683 27.509708687859245 31.304208986588105 26.393209584045817 96-97 14.940453733664855 26.58394098552322 30.974359502615812 27.501245778196115 98-99 15.205556356744063 26.551329858464328 31.03779950143184 27.205314283359773 100-101 15.52910758042232 26.176928455265706 31.72237609432682 26.57158786998515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4651.0 1 2997.0 2 1773.5 3 2122.5 4 1677.5 5 1490.5 6 2377.0 7 4095.0 8 5175.0 9 4867.5 10 3551.0 11 2406.5 12 2152.5 13 2044.0 14 1898.5 15 1725.5 16 1627.0 17 1574.0 18 1530.0 19 1577.0 20 1693.5 21 1792.5 22 1914.0 23 2136.5 24 2403.5 25 2814.5 26 3383.5 27 3996.5 28 4476.0 29 5092.0 30 5926.0 31 6600.0 32 7462.5 33 8479.5 34 9614.5 35 10582.0 36 11256.5 37 11784.0 38 11578.0 39 10890.5 40 10323.0 41 10077.5 42 9967.5 43 9763.5 44 10144.5 45 10844.0 46 12323.5 47 15590.0 48 21120.0 49 20813.0 50 15795.5 51 12708.5 52 9128.0 53 6778.5 54 5783.0 55 5202.0 56 4695.5 57 4134.0 58 3612.0 59 3282.5 60 2948.0 61 2511.0 62 2028.5 63 1439.0 64 945.0 65 727.5 66 588.5 67 454.5 68 330.0 69 242.0 70 212.5 71 187.5 72 143.5 73 145.0 74 129.5 75 85.5 76 71.5 77 51.5 78 34.5 79 26.0 80 19.0 81 14.0 82 11.0 83 7.5 84 3.5 85 1.5 86 0.5 87 0.0 88 1.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3695481983559612 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5752487690310885E-4 16-17 0.0019314365767733163 18-19 2.5752487690310885E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.5752487690310885E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009399658006963473 36-37 0.021117039906054926 38-39 0.012232431652897672 40-41 0.010429757514575907 42-43 0.003347823399740415 44-45 0.002060199015224871 46-47 0.0024464863305795337 48-49 0.0 50-51 5.150497538062177E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.862873153546633E-4 60-61 6.438121922577721E-4 62-63 0.0 64-65 0.0 66-67 0.0012876243845155442 68-69 5.150497538062177E-4 70-71 0.0018026741383217618 72-73 0.0 74-75 0.0 76-77 0.0929664805620223 78-79 0.03463709594346814 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15039452811141557 88-89 0.31997465955211274 90-91 0.2746502812171656 92-93 0.0 94-95 0.0 96-97 1.2876243845155443E-4 98-99 0.0 100-101 6.438121922577721E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 388312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.71579476618872 #Duplication Level Percentage of deduplicated Percentage of total 1 88.61509421202454 53.80335873363761 2 7.388436777287952 8.971896220255521 3 1.635791209204149 2.979550900151244 4 0.6649736222545497 1.6149760789494543 5 0.3750630879583981 1.1386126736427544 6 0.2436046660116606 0.887439054338996 7 0.1670015626928578 0.7097742824264649 8 0.11553441754596987 0.5611811187321808 9 0.08329312453078351 0.45514874290010865 >10 0.6043274721376539 6.808794151182182 >50 0.047926281205281356 2.026874621045309 >100 0.046229775675810526 5.724485176696559 >500 0.0059377693528563985 2.5174344168864398 >1k 0.005513642970509512 6.784145838059867 >5k 0.0012723791470406567 5.016327991095321 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7216 1.8582995117328331 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6270 1.6146809781824927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5994 1.5436041121572344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4798 1.2356043593811161 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2981 0.7676816580481675 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2666 0.6865613218236881 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.6770329013782731 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2215 0.5704176023403861 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1922 0.49496281340777515 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.46560497744082074 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1727 0.44474546241166896 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.3229361956364985 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1198 0.30851480252992436 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1064 0.2740064690249078 RNA PCR Primer, Index 39 (95% over 23bp) CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1044 0.2688559714868456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.2675683471023301 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 831 0.21400317270648342 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 826 0.2127155483219679 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 824 0.2122004985681617 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 796 0.20498980201487466 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 796 0.20498980201487466 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 759 0.1954613815694596 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 709 0.18258513772430415 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 669 0.17228414264817982 RNA PCR Primer, Index 39 (96% over 30bp) GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 646 0.16636107047940832 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 633 0.1630132470796679 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 613 0.1578627495416057 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.14575908032715962 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 556 0.14318383155812853 No Hit ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 552 0.1421537320505161 RNA PCR Primer, Index 39 (96% over 25bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 484 0.12464204042110469 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 477 0.12283936628278293 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 470 0.12103669214446117 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 409 0.10532767465337152 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 407 0.1048126248995653 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 403 0.10378252539195287 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 401 0.10326747563814664 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.5752487690310885E-4 0.0 10-11 0.0 0.0 0.0 2.5752487690310885E-4 0.0 12-13 0.0 0.0 0.0 2.5752487690310885E-4 0.0 14-15 0.0 0.0 0.0 2.5752487690310885E-4 0.0 16-17 0.0 0.0 0.0 7.725746307093266E-4 0.0 18-19 0.0 0.0 0.0 0.00115886194606399 0.0 20-21 0.0 0.0 0.0 0.002188961453676425 0.0 22-23 0.0 0.0 0.0 0.00785450874554482 0.0 24-25 0.0 0.0 0.0 0.01480768042192876 0.0 26-27 0.0 0.0 0.0 0.03180432229753394 0.0 28-29 0.0 0.0 0.0 0.07687117575557799 0.0 30-31 0.0 0.0 0.0 0.18683429819320546 0.0 32-33 0.0 0.0 0.0 0.34096293701971614 0.0 34-35 0.0 0.0 0.0 0.6006767753765014 0.0 36-37 0.0 0.0 0.0 0.9677784874018831 0.0 38-39 0.0 0.0 0.0 1.400677805476009 0.0 40-41 0.0 0.0 0.0 1.8957693813222358 0.0 42-43 0.0 0.0 0.0 2.294417890768248 0.0 44-45 0.0 0.0 0.0 2.6960279362986466 0.0 46-47 0.0 0.0 0.0 3.1772131687921052 0.0 48-49 0.0 0.0 0.0 3.7017913430437384 0.0 50-51 0.0 0.0 0.0 4.232035064587238 0.0 52-53 0.0 0.0 0.0 4.7078122746657325 0.0 54-55 0.0 0.0 0.0 5.209857022188343 0.0 56-57 0.0 0.0 0.0 5.761089021199448 0.0 58-59 0.0 0.0 0.0 6.405158738334123 0.0 60-61 0.0 0.0 0.0 7.143353797976884 0.0 62-63 0.0 0.0 0.0 7.808283030140712 0.0 64-65 0.0 0.0 0.0 8.429175508354106 0.0 66-67 2.5752487690310885E-4 0.0 0.0 9.119857228208247 0.0 68-69 2.5752487690310885E-4 0.0 0.0 9.85264426543604 0.0 70-71 2.5752487690310885E-4 0.0 0.0 10.669384412534251 0.0 72-73 2.5752487690310885E-4 0.0 0.0 11.488699808401492 0.0 74-75 2.5752487690310885E-4 0.0 0.0 12.276983456601908 0.0 76-77 2.5752487690310885E-4 0.0 0.0 13.046081501473042 0.0 78-79 2.5752487690310885E-4 0.0 0.0 13.889475473330723 0.0 80-81 2.5752487690310885E-4 0.0 0.0 14.796478089783474 0.0 82-83 2.5752487690310885E-4 0.0 0.0 15.726786707595954 0.0 84-85 2.5752487690310885E-4 0.0 0.0 16.77787449267599 0.0 86-87 2.5752487690310885E-4 0.0 0.0 17.81569974659552 0.0 88-89 2.5752487690310885E-4 0.0 0.0 18.73416222007046 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTCG 45 9.6555165E-4 42.188114 6 TCACGCA 70 2.060597E-4 33.90116 4 TATCACG 70 2.060597E-4 33.90116 2 GGTATGA 75 3.0893314E-4 31.641087 8 GTATATG 155 1.3096724E-9 30.735178 1 TACTATC 70 0.008417968 27.12093 2 GTATAGC 110 9.2739225E-5 25.985193 1 ATCACGC 95 0.0012308771 24.979805 3 CGCAGAA 210 1.2714736E-9 24.860851 9 CTAGATA 100 0.0016594683 23.730814 4 GGGATGA 180 1.9593426E-7 23.730814 7 GTACAGT 125 2.2645267E-4 22.78158 6 CGGGAGT 125 2.2645267E-4 22.78158 4 ACTGTGC 125 2.2645267E-4 22.78158 8 CACGCAG 105 0.002203949 22.600775 5 GAGTACT 4760 0.0 21.686773 12-13 CATATAC 110 0.0028254539 21.654327 1 TTTAGGC 110 0.0028874818 21.573467 3 ATATGGG 135 3.8104394E-4 21.094057 3 TATAGCC 135 3.8104394E-4 21.094057 2 >>END_MODULE