##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938808_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 386264 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35008698713833 33.0 31.0 34.0 30.0 34.0 2 31.31461642814241 33.0 31.0 34.0 28.0 34.0 3 31.265414328024356 33.0 31.0 34.0 28.0 34.0 4 34.85843102127043 37.0 35.0 37.0 32.0 37.0 5 34.91265818196881 37.0 35.0 37.0 32.0 37.0 6 35.02706180229066 37.0 35.0 37.0 32.0 37.0 7 34.97333688876002 37.0 35.0 37.0 32.0 37.0 8 34.95712258973138 37.0 35.0 37.0 32.0 37.0 9 36.44485895656857 39.0 37.0 39.0 32.0 39.0 10-11 36.32179416150612 39.0 37.0 39.0 32.0 39.0 12-13 36.20234606383199 39.0 37.0 39.0 32.0 39.0 14-15 37.14464459540625 40.0 37.0 41.0 32.0 41.0 16-17 37.07481411676988 40.0 37.0 41.0 31.5 41.0 18-19 37.16842366878612 40.0 37.0 41.0 32.0 41.0 20-21 37.13367282480377 40.0 37.0 41.0 31.5 41.0 22-23 37.015159321086095 40.0 36.0 41.0 31.5 41.0 24-25 36.88513037715138 40.0 36.0 41.0 31.0 41.0 26-27 36.61648121492037 39.5 35.5 41.0 30.5 41.0 28-29 36.34342703435992 39.0 35.0 41.0 30.0 41.0 30-31 35.924938643000644 38.5 35.0 40.5 30.0 41.0 32-33 35.48958354907525 38.0 35.0 40.0 29.5 41.0 34-35 35.07675320506182 38.0 35.0 40.0 26.5 41.0 36-37 34.70216872398153 38.0 35.0 40.0 25.0 41.0 38-39 34.3431461383924 38.0 35.0 40.0 24.0 41.0 40-41 33.976927697119066 37.5 34.5 40.0 22.0 41.0 42-43 33.68785467970093 38.0 34.0 40.0 17.5 41.0 44-45 33.23723023631506 38.0 33.0 40.0 10.0 41.0 46-47 32.520669800965145 37.0 33.0 40.0 2.0 41.0 48-49 31.916212745686884 37.0 32.5 40.0 2.0 41.0 50-51 30.65062755006938 36.0 30.0 39.0 2.0 40.0 52-53 30.63854643456289 35.5 30.5 39.0 2.0 40.0 54-55 31.014562578961538 36.0 31.0 40.0 2.0 41.0 56-57 30.857175403351075 36.0 31.0 40.0 2.0 41.0 58-59 30.631496851894042 36.0 31.0 40.0 2.0 41.0 60-61 30.27957951038668 35.5 29.5 40.0 2.0 41.0 62-63 29.821234958473994 35.0 28.5 39.0 2.0 41.0 64-65 29.370614398442516 35.0 28.0 39.0 2.0 41.0 66-67 28.936793229501067 35.0 26.5 39.0 2.0 40.5 68-69 28.50033137957459 35.0 26.0 38.0 2.0 40.0 70-71 28.017613601060415 35.0 25.5 37.0 2.0 40.0 72-73 27.50908575482054 35.0 24.0 37.0 2.0 39.0 74-75 27.02790966799909 34.0 21.5 36.0 2.0 39.0 76-77 26.49480795518091 34.0 19.0 36.0 2.0 38.5 78-79 26.006757036638156 34.0 15.0 35.0 2.0 37.5 80-81 25.49622020172732 34.0 4.5 35.0 2.0 37.0 82-83 25.02527157591699 33.0 2.0 35.0 2.0 36.5 84-85 24.6159375453058 33.0 2.0 35.0 2.0 36.0 86-87 24.098123304268583 33.0 2.0 35.0 2.0 36.0 88-89 23.555723546589896 32.5 2.0 35.0 2.0 35.0 90-91 23.00784825922167 32.0 2.0 35.0 2.0 35.0 92-93 22.49890877741648 31.5 2.0 35.0 2.0 35.0 94-95 21.961702359008346 31.0 2.0 35.0 2.0 35.0 96-97 21.305750730070628 31.0 2.0 35.0 2.0 35.0 98-99 20.587204606176087 30.0 2.0 34.0 2.0 35.0 100-101 18.734044591264006 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4015639842712204 1101 2 0.2171032161258033 1101 3 0.3813009279951487 1101 4 0.024979713855891816 1101 5 0.005781650803541538 1101 6 0.15032417865622705 1101 7 0.2713057367343623 1101 8 0.271150894101325 1101 9 0.4938652500394767 1101 10-11 0.33166548005312535 1101 12-13 0.196856319565633 1101 14-15 0.5270633263126783 1101 16-17 0.7164202749607469 1101 18-19 0.4868994114817866 1101 20-21 0.38928909762064734 1101 22-23 0.3932690573064477 1101 24-25 0.3738139582483484 1101 26-27 0.5872620137236879 1101 28-29 0.58389967116414 1101 30-31 0.5418770511955273 1101 32-33 0.23998349163954202 1101 34-35 -0.06848404574640199 1101 36-37 -0.2983344862891215 1101 38-39 0.131091236373841 1101 40-41 -0.07436881545316254 1101 42-43 0.251241538353014 1101 44-45 0.4154083966338433 1101 46-47 0.5122323707991399 1101 48-49 0.9307894309340732 1101 50-51 0.8824288093419348 1101 52-53 0.9926087511326109 1101 54-55 0.9266679236578135 1101 56-57 0.7170971909951795 1101 58-59 1.0114716035312945 1101 60-61 0.8140337937809399 1101 62-63 0.5240560395529421 1101 64-65 0.5578324092135283 1101 66-67 0.22954813775226413 1101 68-69 0.15784161099440297 1101 70-71 0.1452460476208337 1101 72-73 0.22374892147205472 1101 74-75 -0.017710406919920985 1101 76-77 0.27566095520412404 1101 78-79 0.006221003395751268 1101 80-81 -0.5419770749356907 1101 82-83 -0.673482648845674 1101 84-85 -0.6578752499664375 1101 86-87 -0.5057938546084806 1101 88-89 -0.5017441428044407 1101 90-91 -0.36624862238931755 1101 92-93 -0.6429255655340214 1101 94-95 -0.5289554439873037 1101 96-97 -0.26743516206313345 1101 98-99 -0.21528443640337258 1101 100-101 -0.33666078568821334 1102 1 0.269935196392435 1102 2 -0.022381632359046222 1102 3 0.12081607951029838 1102 4 0.028987289613468192 1102 5 -0.11798350071160968 1102 6 0.07806660289865164 1102 7 0.027040585219211266 1102 8 0.32642362137405456 1102 9 0.3405925227667481 1102 10-11 0.04149881338646111 1102 12-13 0.2188941983535173 1102 14-15 0.10660499297934223 1102 16-17 0.24507936586984158 1102 18-19 0.3157744114817831 1102 20-21 0.26110727943883205 1102 22-23 0.13802663306402252 1102 24-25 0.13117001885441226 1102 26-27 0.2343339834206617 1102 28-29 0.3346269438914149 1102 30-31 0.29372932392279694 1102 32-33 0.3065592492153044 1102 34-35 0.028379590617234385 1102 36-37 -0.10722463780426494 1102 38-39 0.10633366061626504 1102 40-41 0.08818421484986771 1102 42-43 0.27715820501968125 1102 44-45 -0.18162569427524744 1102 46-47 0.05283464352641687 1102 48-49 -0.17157420542956814 1102 50-51 0.09291365782678085 1102 52-53 -0.12405033977648472 1102 54-55 -0.341646470281578 1102 56-57 -0.45383841506542666 1102 58-59 -0.2687177904081004 1102 60-61 -0.3472806001584523 1102 62-63 -0.46630002105311874 1102 64-65 -0.6909971362410197 1102 66-67 -1.017421559217432 1102 68-69 -0.8135636920359026 1102 70-71 -0.899753952379168 1102 72-73 -0.970417745194613 1102 74-75 -0.7737028311623462 1102 76-77 -1.0311420750989058 1102 78-79 -1.3104949056951547 1102 80-81 -0.9557308628144767 1102 82-83 -0.9285281033911268 1102 84-85 -0.9994282802694663 1102 86-87 -1.0130968849115156 1102 88-89 -1.115043385228681 1102 90-91 -1.1790402890559868 1102 92-93 -1.4690732928067476 1102 94-95 -1.6703872621691254 1102 96-97 -1.531802586305563 1102 98-99 -2.0189662545851945 1102 100-101 -1.6944941190215452 1103 1 -0.13163960675717234 1103 2 -0.09299580558739251 1103 3 -0.056422765634057015 1103 4 0.05619988803866249 1103 5 0.3358117748789411 1103 6 -0.08963943384675588 1103 7 0.007995965796638416 1103 8 0.07191443502234307 1103 9 -0.16844684731199067 1103 10-11 -0.02906286640357081 1103 12-13 0.09245850281545387 1103 14-15 0.003728352559399184 1103 16-17 0.16223422151288247 1103 18-19 0.13416811226919378 1103 20-21 0.3276689592288591 1103 22-23 0.3538691527490627 1103 24-25 0.06721201360506512 1103 26-27 0.04034973145216014 1103 28-29 0.03707313811713675 1103 30-31 0.17783431079944023 1103 32-33 0.19080071903157858 1103 34-35 0.5404845774938849 1103 36-37 0.8922189317495395 1103 38-39 0.6823021645532705 1103 40-41 0.4888246347973748 1103 42-43 0.812675265387135 1103 44-45 0.4537233870895747 1103 46-47 0.31641469601985506 1103 48-49 0.20059377357305763 1103 50-51 0.44154357908662334 1103 52-53 0.37750346599779405 1103 54-55 0.5240753144953239 1103 56-57 0.5766287765356211 1103 58-59 0.6351667240275951 1103 60-61 0.6932653316000774 1103 62-63 0.43006743301512174 1103 64-65 0.7238690054912631 1103 66-67 0.4978015378954268 1103 68-69 0.4741108486465144 1103 70-71 0.28402557517988924 1103 72-73 0.3197134884011881 1103 74-75 0.47807669639671246 1103 76-77 0.5693408645336397 1103 78-79 0.3741560129400163 1103 80-81 0.43593317918027097 1103 82-83 0.7694456498897111 1103 84-85 0.6335900924339448 1103 86-87 0.4176432725687995 1103 88-89 0.11578076175294783 1103 90-91 -0.013927428163594158 1103 92-93 0.2469687019439064 1103 94-95 0.41820066434006264 1103 96-97 0.3870215606760681 1103 98-99 0.565779152238953 1103 100-101 0.9663247786162508 1104 1 0.4243211613047144 1104 2 0.5866008237813034 1104 3 0.22579853565065022 1104 4 0.44032062294679974 1104 5 0.491876148411194 1104 6 0.570838532723215 1104 7 0.6633212869735914 1104 8 0.5457920424266831 1104 9 0.9060662069772789 1104 10-11 0.8007444274215487 1104 12-13 0.770929286072807 1104 14-15 0.8331488526284687 1104 16-17 0.8523951553435225 1104 18-19 0.8206165167449484 1104 20-21 0.9250546478598807 1104 22-23 0.7892020716605117 1104 24-25 0.8369419486789695 1104 26-27 0.8277374921925897 1104 28-29 0.9605743123124668 1104 30-31 0.9308872186596417 1104 32-33 0.9782610036012684 1104 34-35 0.9961690643014549 1104 36-37 0.8372665902659122 1104 38-39 0.9193687483355646 1104 40-41 0.47446491660424783 1104 42-43 0.7956494330898565 1104 44-45 0.7516374636194882 1104 46-47 1.0081504330000968 1104 48-49 0.957583689307274 1104 50-51 1.1379312016864311 1104 52-53 1.0868970286445716 1104 54-55 0.9676517753324596 1104 56-57 0.7198633393205363 1104 58-59 0.7068962446796228 1104 60-61 0.7892281717713701 1104 62-63 0.8661912070170565 1104 64-65 0.8582660216537157 1104 66-67 0.43696440569484807 1104 68-69 0.367366132525504 1104 70-71 0.595246047620833 1104 72-73 0.6823015530510013 1104 74-75 0.5081568179604616 1104 76-77 0.7442614336730209 1104 78-79 0.4249261469364214 1104 80-81 0.3381814178872773 1104 82-83 0.35815610713519064 1104 84-85 -0.16986687676069678 1104 86-87 0.07267504491304777 1104 88-89 0.06334257968359935 1104 90-91 -0.04437362238931897 1104 92-93 0.08687407561430405 1104 94-95 -0.13628199901122784 1104 96-97 -0.04173241086696322 1104 98-99 0.44482321909901756 1104 100-101 0.2784707932591566 1105 1 -0.2671018406446031 1105 2 -0.10691073288814579 1105 3 0.016943063637281597 1105 4 -0.05253651991034758 1105 5 -0.07976127848939285 1105 6 -0.17179583096378082 1105 7 -0.02620809202947072 1105 8 -0.11797849502806201 1105 9 -0.22150271532849075 1105 10-11 -0.158744572856925 1105 12-13 -0.05448146302214951 1105 14-15 -0.1681251657508156 1105 16-17 -0.08569312090264702 1105 18-19 0.07874795645533084 1105 20-21 -0.05852235019079899 1105 22-23 0.3215398605772464 1105 24-25 0.4071382728226638 1105 26-27 0.41501123209791047 1105 28-29 0.35662694389141336 1105 30-31 0.3391578953513701 1105 32-33 0.38049575715181305 1105 34-35 0.316887527125175 1105 36-37 0.5135266849470526 1105 38-39 0.5017622320448396 1105 40-41 0.9375810402466911 1105 42-43 1.263973019834495 1105 44-45 1.2556335649840094 1105 46-47 1.1063055429973119 1105 48-49 0.9849019850466227 1105 50-51 1.226945403858526 1105 52-53 1.3145634168372702 1105 54-55 1.3023217836866756 1105 56-57 1.1964367172097035 1105 58-59 1.187472685782378 1105 60-61 1.3437881829103304 1105 62-63 0.9265782858251903 1105 64-65 0.9373943981505128 1105 66-67 1.0140599222640496 1105 68-69 1.072817260345051 1105 70-71 1.1551666825414664 1105 72-73 0.9542383394614724 1105 74-75 0.9642971688376534 1105 76-77 1.2397362317793998 1105 78-79 1.3738331366328893 1105 80-81 1.2006500895664765 1105 82-83 1.1692602563972336 1105 84-85 1.0887727779315917 1105 86-87 0.8666808928662668 1105 88-89 0.5608084666231719 1105 90-91 0.18525600724031221 1105 92-93 0.5538261780927201 1105 94-95 0.5171153833335218 1105 96-97 0.484165667662694 1105 98-99 0.33062104700211137 1105 100-101 0.5188286323012044 1106 1 -0.09573644539861093 1106 2 -0.033744816438648684 1106 3 -0.2831043184996531 1106 4 -0.18579878003827588 1106 5 -0.02337653553747998 1106 6 -0.19564483988741443 1106 7 0.0938266548709521 1106 8 -0.06874055773042897 1106 9 -0.1824124523576316 1106 10-11 -0.06603352492199832 1106 12-13 -0.007533662343000458 1106 14-15 0.12990847556640972 1106 16-17 0.25431319671561425 1106 18-19 0.10342615277532019 1106 20-21 0.03503762769754104 1106 22-23 0.026862453959545007 1106 24-25 -0.19011356323514406 1106 26-27 -0.1421237280221277 1106 28-29 -0.4257113645662969 1106 30-31 -0.4586388352811781 1106 32-33 -0.731918362724997 1106 34-35 -0.9479786183379844 1106 36-37 -0.5368365781027791 1106 38-39 -0.4975270359011432 1106 40-41 -0.3940346906227745 1106 42-43 -0.5160159243335585 1106 44-45 -0.48745654004639505 1106 46-47 -0.6202797843342864 1106 48-49 -0.5277135089121536 1106 50-51 -0.45192216306874045 1106 52-53 -0.5378214840550903 1106 54-55 -0.5722932364507329 1106 56-57 -0.3413110518813447 1106 58-59 -0.24021032772153106 1106 60-61 -0.2589323414519846 1106 62-63 -0.1688572349834665 1106 64-65 -0.03232051932559443 1106 66-67 0.1233744606830669 1106 68-69 0.4077497407999182 1106 70-71 0.32870375906361815 1106 72-73 0.22157230455663424 1106 74-75 0.01777975590233183 1106 76-77 0.11444996470208935 1106 78-79 0.2780225072401663 1106 80-81 0.32911490832980306 1106 82-83 0.49106393640986923 1106 84-85 0.4099249535613758 1106 86-87 0.3003459011581384 1106 88-89 0.24751382870166694 1106 90-91 0.3951786164166542 1106 92-93 0.2732252146559375 1106 94-95 0.7372843796219222 1106 96-97 0.39935164255016176 1106 98-99 0.7939591185491359 1106 100-101 1.1916352342122813 1107 1 0.12076852972576546 1107 2 0.31028503430761845 1107 3 0.41198274617696384 1107 4 0.5128206229467978 1107 5 0.2513498326217203 1107 6 -0.07376673043467719 1107 7 0.46437391855253907 1107 8 0.410923621374053 1107 9 0.22409252276675318 1107 10-11 0.3654154800531231 1107 12-13 0.4423108650201826 1107 14-15 0.46393832631267884 1107 16-17 0.7648293658698364 1107 18-19 0.5100244114817869 1107 20-21 0.41110727943883774 1107 22-23 0.2498599663973593 1107 24-25 0.143586685521079 1107 26-27 0.37425065008732616 1107 28-29 0.4216269438914111 1107 30-31 0.3964793239227973 1107 32-33 0.4146425825486304 1107 34-35 0.17537959061723996 1107 36-37 0.5453586955290604 1107 38-39 0.3570003272829325 1107 40-41 0.6792675481831978 1107 42-43 0.45749153835301115 1107 44-45 0.44762430572475154 1107 46-47 0.49058464352641096 1107 48-49 0.6681757945704341 1107 50-51 0.8729969911601181 1107 52-53 0.8804496602235155 1107 54-55 0.9937701963850891 1107 56-57 1.0599949182679076 1107 58-59 1.1191988762585687 1107 60-61 1.004886066508213 1107 62-63 1.093033312280216 1107 64-65 1.0305028637589793 1107 66-67 0.9834117741159005 1107 68-69 1.1483529746307646 1107 70-71 1.2727460476208314 1107 72-73 1.4937489214720543 1107 74-75 1.3838805021709888 1107 76-77 1.4107745915677619 1107 78-79 1.085255094304845 1107 80-81 0.8428524705188529 1107 82-83 0.946971896608872 1107 84-85 1.0659883863971977 1107 86-87 0.949319781755154 1107 88-89 0.7772899481046522 1107 90-91 0.8818763776106842 1107 92-93 0.7979267071932519 1107 94-95 0.6891127378308752 1107 96-97 0.6885307470277731 1107 98-99 1.0502837454148093 1107 100-101 0.8795892143117854 1108 1 0.37338347225450264 1108 2 0.6171815860317587 1108 3 0.3489080335332879 1108 4 0.3862976344410498 1108 5 0.4336486831964308 1108 6 0.5178137293354368 1108 7 0.4498911599318518 1108 8 0.4237109776958903 1108 9 0.40739712046789833 1108 10-11 0.5364068593634741 1108 12-13 0.6701125891581086 1108 14-15 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-0.9181555438890001 2316 94-95 -1.1976513314331925 2316 96-97 -1.220019036522011 2316 98-99 -1.2880658216847607 2316 100-101 -1.5766499631773918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4498.0 3 824.0 4 690.0 5 659.0 6 775.0 7 885.0 8 1060.0 9 1308.0 10 1735.0 11 2292.0 12 3601.0 13 4881.0 14 5246.0 15 6332.0 16 7538.0 17 9269.0 18 8018.0 19 4960.0 20 4700.0 21 4705.0 22 4740.0 23 5089.0 24 5424.0 25 5936.0 26 6667.0 27 7138.0 28 8012.0 29 9136.0 30 10590.0 31 12161.0 32 14133.0 33 17257.0 34 23012.0 35 36759.0 36 39143.0 37 52009.0 38 47163.0 39 7916.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.39363632352063 19.572795776238223 14.827553089041116 17.206014811200028 2 15.127752321531835 24.33789989860721 39.81391440996398 20.720433369896973 3 20.455960323306943 31.403625271065273 31.074456053704647 17.065958351923136 4 11.56990719492178 19.5015130417195 38.14135725157981 30.787222511778907 5 10.986863617254283 41.417753579936786 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18.689925702706915 28.066971476074194 36.49763599522004 16.745466825998857 28-29 16.85919827552624 29.2904725421704 37.287719617187605 16.562609565115764 30-31 17.006979352748118 30.74641165718084 36.07998213618047 16.166626853890573 32-33 16.431104113547292 30.69895806044131 35.55913791627651 17.310799909734886 34-35 17.184053622228284 29.676681864827298 35.6150059103958 17.524258602548613 36-37 17.92553108545707 29.653332570988987 35.802592663821734 16.61854367973221 38-39 18.50023320986419 29.044177353828083 35.772314416267264 16.68327502004046 40-41 17.026957878072 29.738992881624043 36.62697294341793 16.60707629688602 42-43 16.408932587262136 31.21247828758732 35.0511419072625 17.32744721788804 44-45 16.29496332772476 31.33572834581728 33.97002553091969 18.39928279553826 46-47 16.78841506197864 30.543904784179205 32.87271640549882 19.79496374834334 48-49 17.562272654614354 30.677380409341904 32.67860461429097 19.08174232175277 50-51 17.830080919417153 31.313098497656366 32.601805515307376 18.255015067619105 52-53 16.600046261763612 31.344813874370725 33.04272455797925 19.01241530588642 54-55 16.012500324852518 30.905818758283736 33.28932664570285 19.792354271160892 56-57 17.00489907591814 31.460875597769107 31.283542300811835 20.25068302550092 58-59 17.11482138102567 30.141505451758917 31.785020813445293 20.958652353770123 60-61 17.297904385987692 29.227972681710767 32.88322210392376 20.59090082837778 62-63 18.62621286061003 28.941829464132585 32.523763492830675 19.90819418242671 64-65 17.88127068132371 29.42213164824139 33.24140189806788 19.455195772367023 66-67 17.04939808833583 29.83433456982357 32.85247120416126 20.263796137679332 68-69 16.843182060420776 30.403281782519514 32.79832791308891 19.95520824397079 70-71 17.345885692598042 29.803448042798987 31.878148704644353 20.972517559958614 72-73 17.113651280518315 29.345541742752612 31.74138793758895 21.799419039140126 74-75 17.218680754560044 29.499320947943673 32.41775022580918 20.864248071687104 76-77 17.88312251968013 28.275191733436593 32.90668149769088 20.935004249192392 78-79 17.15142752100977 28.451298519930212 33.61368009031375 20.783593868746273 80-81 17.349355638793938 29.460539940780357 32.608743667798315 20.5813607526274 82-83 17.221651219268168 28.834681867617117 32.36456293585897 21.57910397725575 84-85 17.59703218596915 27.864447303109447 32.48041164776049 22.058108863160903 86-87 18.132349310707742 27.897561165754976 32.46936839780154 21.500721125735744 88-89 18.783939540027053 27.020259444596768 32.47670443249256 21.71909658288362 90-91 18.2368071160927 27.146999458026983 33.051645999342355 21.56454742653796 92-93 18.34915923027135 27.819483399054345 32.27209278749802 21.559264583176287 94-95 18.582772143129173 27.61705706267783 31.68843187794324 22.111738916249752 96-97 18.844633579818783 26.65032384522499 31.28333175190973 23.2217108230465 98-99 19.561925582979253 26.32579770122711 31.36240571794735 22.74987099784628 100-101 20.530329708313463 25.535274098542153 32.21780266360344 21.716593529540944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4822.0 1 3133.0 2 1884.0 3 2242.5 4 1764.5 5 1573.0 6 2478.0 7 4115.5 8 5391.0 9 5245.0 10 3739.0 11 2496.0 12 2279.0 13 2218.5 14 2063.0 15 1821.5 16 1683.0 17 1685.0 18 1673.0 19 1690.0 20 1759.5 21 1935.5 22 2197.5 23 2446.5 24 2757.0 25 3192.0 26 3752.0 27 4427.0 28 5001.0 29 5575.5 30 6207.5 31 6840.5 32 7596.5 33 8292.0 34 9145.5 35 10077.0 36 10835.5 37 11334.0 38 11105.5 39 10260.5 40 9585.5 41 9311.5 42 9026.0 43 9080.0 44 9577.0 45 10246.5 46 11989.5 47 15247.0 48 21262.5 49 20536.5 50 13891.0 51 10693.0 52 8435.5 53 7146.5 54 6285.5 55 5540.0 56 4949.5 57 4289.5 58 3823.0 59 3487.5 60 3068.0 61 2696.0 62 2224.5 63 1650.0 64 1173.5 65 893.5 66 724.5 67 587.5 68 462.5 69 348.5 70 299.0 71 263.5 72 220.0 73 193.5 74 159.0 75 131.5 76 94.0 77 67.5 78 72.0 79 57.5 80 38.5 81 27.5 82 22.0 83 18.5 84 12.5 85 6.5 86 5.5 87 5.0 88 3.0 89 3.5 90 2.5 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2627736470393306 2 0.1643953358324876 3 0.19364993890189092 4 0.15973531056479506 5 0.23869684982291903 6 0.1941677194871901 7 0.24076797216411572 8 0.24801690035830418 9 0.14497856388376862 10-11 0.20465277633949838 12-13 0.21047780792411408 14-15 0.1708675931487273 16-17 0.2918988049624091 18-19 0.23235403765300416 20-21 0.19623884182838677 22-23 0.24491021684650913 24-25 0.23054180560445706 26-27 0.34277074746805297 28-29 0.31558726673984633 30-31 0.29202825010873396 32-33 0.18976658451214712 34-35 0.3492430047842926 36-37 0.384710974877286 38-39 0.3669769898307893 40-41 0.3299556779818984 42-43 0.3633525257336951 44-45 0.3718959053911315 46-47 0.37642648551249924 48-49 0.39467825114429506 50-51 0.38794710353540585 52-53 0.38755876809643147 54-55 0.38160429136549096 56-57 0.3612814033924984 58-59 0.33565126442018933 60-61 0.30393720357061493 62-63 0.37021311848890914 64-65 0.4030921856554067 66-67 0.4072344303378 68-69 0.412930016776091 70-71 0.40956444297164635 72-73 0.4029627405090819 74-75 0.3977849346560901 76-77 0.3844520845846364 78-79 0.3581747198807034 80-81 0.369048112171986 82-83 0.3788859432926703 84-85 0.380309839902243 86-87 0.3751320340492513 88-89 0.38328707826771324 90-91 0.4034805210943811 92-93 0.4030921856554067 94-95 0.4117650104591678 96-97 0.4115061201665183 98-99 0.409176107532672 100-101 0.38432263943831163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 386264.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.136305427365244 #Duplication Level Percentage of deduplicated Percentage of total 1 89.52965705263225 56.52571772482254 2 6.811344599454215 8.600862660043523 3 1.4626713476225648 2.7704299483006256 4 0.6391537467662203 1.614152246835078 5 0.331560008502633 1.046673698216103 6 0.22221176671764847 0.8417777983823935 7 0.1608309320674637 0.7107989594425455 8 0.11020598363176337 0.5566398913998581 9 0.09257356281631604 0.5260277462823281 >10 0.5460520194401428 6.362972818453634 >50 0.04111358787235492 1.7671934359957149 >100 0.04193585962965767 5.281333379652345 >500 0.004933630544665608 2.1653830262980374 >1k 0.004522494665943475 5.957269294358661 >5k 0.001233407636166402 5.272767371516666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8113 2.100376944266098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6116 1.5833730298448727 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6084 1.5750885404800863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4569 1.1828697471159622 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2991 0.7743408653149142 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.6899426299111489 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2508 0.6492968539651638 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2094 0.5421162728082348 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1963 0.5082016444711389 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1739 0.45021021891763147 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.3495018950769422 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1044 0.27028146552616866 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.2630325373319802 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1011 0.26173808586873226 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 860 0.22264565167864464 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 853 0.22083341963009756 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 815 0.21099558850941327 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 768 0.19882774475488266 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 755 0.19546217095043802 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.18691879129300168 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 690 0.1786343019282149 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 633 0.16387755524718844 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 601 0.15559306588240168 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 570 0.14756746681026447 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 552 0.14290744154257193 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 523 0.1353996230557339 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 475 0.12297288900855374 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 455 0.117795083155562 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 417 0.10795725203487772 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 398 0.10303833647453556 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 398 0.10303833647453556 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.294451463247934E-4 0.0 18-19 0.0 0.0 0.0 5.177805852991736E-4 0.0 20-21 0.0 0.0 0.0 0.002459457780171075 0.0 22-23 0.0 0.0 0.0 0.007378373340513224 0.0 24-25 0.0 0.0 0.0 0.014627301534701655 0.0 26-27 0.0 0.0 0.0 0.03054905453265124 0.0 28-29 0.0 0.0 0.0 0.08142099703829506 0.0 30-31 0.0 0.0 0.0 0.1785048567818901 0.0 32-33 0.0 0.0 0.0 0.31545782159352154 0.0 34-35 0.0 0.0 0.0 0.5548018971480646 0.0 36-37 0.0 0.0 0.0 0.890970942153553 0.0 38-39 0.0 0.0 0.0 1.3128326740260547 0.0 40-41 0.0 0.0 0.0 1.7798707619659093 0.0 42-43 0.0 0.0 0.0 2.173642897085931 0.0 44-45 0.0 0.0 0.0 2.5750522958391153 0.0 46-47 2.588902926495868E-4 0.0 0.0 3.0417020483399955 0.0 48-49 2.588902926495868E-4 0.0 0.0 3.5240146635461755 0.0 50-51 2.588902926495868E-4 0.0 0.0 4.042442474576973 0.0 52-53 2.588902926495868E-4 0.0 0.0 4.5291562247581965 0.0 54-55 2.588902926495868E-4 0.0 0.0 5.005255472940787 0.0 56-57 2.588902926495868E-4 0.0 0.0 5.51979992958184 0.0 58-59 2.588902926495868E-4 0.0 0.0 6.144502205745294 0.0 60-61 2.588902926495868E-4 0.0 0.0 6.871983928090632 0.0 62-63 2.588902926495868E-4 0.0 0.0 7.516102976202804 0.0 64-65 2.588902926495868E-4 0.0 0.0 8.10145392788352 0.0 66-67 2.588902926495868E-4 0.0 0.0 8.744925750264068 0.0 68-69 2.588902926495868E-4 0.0 0.0 9.453508481245986 0.0 70-71 2.588902926495868E-4 0.0 0.0 10.246618892777995 0.0 72-73 5.177805852991736E-4 0.0 0.0 11.065877223867613 0.0 74-75 5.177805852991736E-4 0.0 0.0 11.864424331545266 0.0 76-77 5.177805852991736E-4 0.0 0.0 12.632292939543937 0.0 78-79 5.177805852991736E-4 0.0 0.0 13.469285255680052 0.0 80-81 6.47225731623967E-4 0.0 0.0 14.384850775635318 0.0 82-83 7.766708779487604E-4 0.0 0.0 15.305076320858273 0.0 84-85 7.766708779487604E-4 0.0 0.0 16.34672141333389 0.0 86-87 0.0010355611705983472 0.0 0.0 17.378399229542488 0.0 88-89 0.0010355611705983472 0.0 0.0 18.29875421991177 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGCC 65 0.005880789 29.188456 3 TAGATTG 65 0.005880789 29.188456 5 GTCGGGA 170 3.6816346E-9 27.90073 2 GGTCGGG 180 6.6429493E-9 26.398682 1 GGGAACG 340 0.0 25.110659 7 ATGGGAG 795 0.0 25.058016 5 GTATACT 100 0.0016656399 23.715622 4 AGTACTC 120 1.7247951E-4 23.715622 5 AGTACTT 4760 0.0 23.58606 12-13 CATGGGT 405 0.0 23.422836 4 GGAACGC 365 0.0 23.39075 8 CGGGAGT 205 2.6988346E-8 23.137192 4 GAACGCA 370 0.0 23.071663 9 GAGTACT 4735 0.0 22.95867 12-13 GCCGTAT 105 3.4195182E-8 22.63333 94-95 CCGTATC 65 2.927758E-4 21.93692 94-95 CGCAGAA 175 4.093323E-6 21.680038 9 AGTCACG 55 0.0028681557 21.601732 68-69 ATGGGAT 825 0.0 21.272194 5 ACAGTGG 335 1.8189894E-12 21.23787 6 >>END_MODULE