##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938808_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 386264 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880263239649565 31.0 31.0 34.0 30.0 34.0 2 32.01449526748545 33.0 31.0 34.0 30.0 34.0 3 32.047718658741175 33.0 31.0 34.0 30.0 34.0 4 35.74027090280223 37.0 35.0 37.0 33.0 37.0 5 35.68008926537291 37.0 35.0 37.0 33.0 37.0 6 35.75759066338048 37.0 35.0 37.0 33.0 37.0 7 35.66806640018226 37.0 35.0 37.0 33.0 37.0 8 35.69511266905536 37.0 35.0 37.0 33.0 37.0 9 37.24513286249819 39.0 37.0 39.0 33.0 39.0 10-11 37.18398556427728 39.0 37.0 39.0 33.0 39.0 12-13 37.091291189445556 39.0 37.0 39.0 33.0 39.0 14-15 38.08016926247333 40.0 37.0 41.0 33.0 41.0 16-17 38.05205248224013 40.0 37.0 41.0 33.0 41.0 18-19 38.2544555019365 40.0 37.0 41.0 33.0 41.0 20-21 38.26892229148976 40.0 37.5 41.0 33.0 41.0 22-23 38.11944550877121 40.0 37.0 41.0 33.0 41.0 24-25 37.966842625768905 40.0 37.0 41.0 33.0 41.0 26-27 37.62097684485222 40.0 36.0 41.0 32.5 41.0 28-29 37.32222780274631 39.5 35.5 41.0 32.0 41.0 30-31 36.91306852308266 39.0 35.0 41.0 31.5 41.0 32-33 36.426820775428205 38.0 35.0 41.0 31.0 41.0 34-35 36.138138682351965 38.0 35.0 41.0 30.0 41.0 36-37 35.8042582275335 38.0 35.0 41.0 30.0 41.0 38-39 35.45722226249404 38.0 35.0 40.5 29.5 41.0 40-41 35.070969595924026 38.0 35.0 40.0 26.5 41.0 42-43 34.68069377420624 38.0 35.0 40.0 24.5 41.0 44-45 34.16598621668082 38.0 34.0 40.0 22.5 41.0 46-47 33.42816571049852 38.0 33.0 40.0 13.5 41.0 48-49 32.808610173352946 38.0 33.0 40.0 2.0 41.0 50-51 32.41736869084357 37.0 33.0 40.0 2.0 41.0 52-53 32.08008382867676 37.0 32.5 40.0 2.0 41.0 54-55 31.750432346788728 37.0 32.0 40.0 2.0 41.0 56-57 31.408420147878136 36.0 31.0 40.0 2.0 41.0 58-59 31.03101505705942 36.0 31.0 40.0 2.0 41.0 60-61 30.550501729387157 35.0 30.5 39.5 2.0 41.0 62-63 30.080219746080402 35.0 29.0 39.0 2.0 41.0 64-65 29.552024004307935 35.0 28.0 39.0 2.0 40.0 66-67 29.058050970320814 35.0 27.5 38.0 2.0 40.0 68-69 28.733179897686554 35.0 27.0 38.0 2.0 40.0 70-71 28.29052021415405 35.0 26.0 37.0 2.0 39.5 72-73 27.800183294327198 34.0 26.0 36.5 2.0 39.0 74-75 27.326701427003293 34.0 24.5 36.0 2.0 39.0 76-77 25.49714573452354 31.5 20.5 34.5 2.0 36.5 78-79 26.30367184102065 33.5 22.0 35.0 2.0 37.0 80-81 26.31526235942257 34.0 21.5 35.0 2.0 37.0 82-83 25.979014352877826 33.5 20.0 35.0 2.0 36.5 84-85 25.591890520473044 33.0 17.5 35.0 2.0 36.0 86-87 25.0009967276267 33.0 7.5 35.0 2.0 35.5 88-89 24.39245697243336 32.5 2.0 35.0 2.0 35.0 90-91 23.861941573638752 32.0 2.0 35.0 2.0 35.0 92-93 23.57674414390158 32.0 2.0 35.0 2.0 35.0 94-95 23.256183594639936 32.0 2.0 35.0 2.0 35.0 96-97 22.682507300706252 31.0 2.0 34.0 2.0 35.0 98-99 21.967958442930225 31.0 2.0 34.0 2.0 35.0 100-101 19.095118882422383 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13011381080605489 1101 2 0.1772045730682592 1101 3 -0.2677266852009801 1101 4 0.08061431091264382 1101 5 -0.07286398718139964 1101 6 0.13850901975534669 1101 7 0.2623266753251272 1101 8 0.2667185514046153 1101 9 0.09549623399237817 1101 10-11 0.28196571834969575 1101 12-13 0.08362522697620989 1101 14-15 0.21327012333479445 1101 16-17 0.22951173196202745 1101 18-19 0.14979212246205265 1101 20-21 0.3607383150406065 1101 22-23 0.08127359192258155 1101 24-25 0.3297364581594806 1101 26-27 0.3385562884344111 1101 28-29 0.26955722522696846 1101 30-31 0.014026388848655813 1101 32-33 0.3367495881014193 1101 34-35 0.4420759837512733 1101 36-37 0.18141336588243462 1101 38-39 0.6750934251020411 1101 40-41 0.5196568198750597 1101 42-43 0.47279583127783553 1101 44-45 -0.10103088310188468 1101 46-47 0.2931977239960091 1101 48-49 0.12646062434775018 1101 50-51 0.16064688594139653 1101 52-53 0.07723851463611453 1101 54-55 0.018069857092889663 1101 56-57 0.08986169114700715 1101 58-59 0.014876225031908774 1101 60-61 -0.32375177020590584 1101 62-63 -0.47801510596740115 1101 64-65 -0.4944795666537587 1101 66-67 -0.7614128709256676 1101 68-69 -0.5404531439386737 1101 70-71 -0.3724840723729166 1101 72-73 -0.22753363995707332 1101 74-75 -0.3171985708269496 1101 76-77 -0.6503593515235089 1101 78-79 -0.7927769669917417 1101 80-81 -0.7381440491833011 1101 82-83 -0.42414168452084056 1101 84-85 -0.5061666327960204 1101 86-87 -0.44808438960664887 1101 88-89 -0.5953325108739236 1101 90-91 -0.4628380298314241 1101 92-93 -0.19482586999062335 1101 94-95 -0.6038741210698468 1101 96-97 -0.45953160529152015 1101 98-99 -0.4656748199616523 1101 100-101 -0.01987495251898963 1102 1 0.34970471989695895 1102 2 0.2768333609470446 1102 3 0.15780361782932317 1102 4 0.23686431091264382 1102 5 0.19743904312163352 1102 6 0.2319105349068593 1102 7 0.18882667532512443 1102 8 0.3106049150409831 1102 9 0.09951138550753313 1102 10-11 0.11848844562242533 1102 12-13 0.29997749970348053 1102 14-15 0.2962473960620642 1102 16-17 0.3324890046892932 1102 18-19 0.2247807588256947 1102 20-21 0.36645801201029826 1102 22-23 0.2153341979831893 1102 24-25 0.4681910036140309 1102 26-27 0.2907040157071421 1102 28-29 0.6097163161360655 1102 30-31 0.4504619949092543 1102 32-33 0.3177306487074816 1102 34-35 0.46183355950883964 1102 36-37 0.5485156386097074 1102 38-39 0.7617449402535499 1102 40-41 0.7878500016932506 1102 42-43 0.8304549221869308 1102 44-45 0.7048706320496336 1102 46-47 0.31087575429904035 1102 48-49 -0.10072876959164034 1102 50-51 -0.027856901937390433 1102 52-53 -0.2961705762729778 1102 54-55 -0.40683165805862487 1102 56-57 -0.24646027854996433 1102 58-59 -0.1414798355741489 1102 60-61 -0.1358085883877287 1102 62-63 -0.4061704089977063 1102 64-65 -0.1628015363507238 1102 66-67 0.15449621998342522 1102 68-69 -0.055812992423522445 1102 70-71 0.17665607914223713 1102 72-73 0.2796746933762577 1102 74-75 0.222862035233657 1102 76-77 0.20400807271891708 1102 78-79 -0.20077696699174297 1102 80-81 0.1220226174833634 1102 82-83 -0.005073502702657606 1102 84-85 0.3173295793251931 1102 86-87 0.34219970130244093 1102 88-89 0.3850652163988002 1102 90-91 0.29940060653221323 1102 92-93 0.5100529178881636 1102 94-95 0.12057284862711981 1102 96-97 -0.15210357498849092 1102 98-99 0.2259236648868317 1102 100-101 0.6587841383901001 1103 1 -0.08202231422377437 1103 2 0.08311157617013976 1103 3 0.19622881467971354 1103 4 0.14237612193626603 1103 5 0.149040092990397 1103 6 0.12421499684911907 1103 7 -6.326422601716786E-4 1103 8 -0.10184652852856857 1103 9 0.0831334327516231 1103 10-11 -0.09402599007311352 1103 12-13 0.07538957319429329 1103 14-15 0.08516078188883824 1103 16-17 0.047596616237846945 1103 18-19 -0.05681504169924523 1103 20-21 -0.07459185675610058 1103 22-23 0.2175179250173116 1103 24-25 -0.029992723420093625 1103 26-27 0.19210034116645858 1103 28-29 0.5065457124615236 1103 30-31 0.22322839910872716 1103 32-33 0.2294996775788718 1103 34-35 0.029644583130895796 1103 36-37 0.38011668847847346 1103 38-39 -0.1314414114524851 1103 40-41 0.2298132562864268 1103 42-43 0.282454922186929 1103 44-45 0.4767026530470133 1103 46-47 0.3534794288397194 1103 48-49 -0.12090724728193436 1103 50-51 -0.15762068146494812 1103 52-53 0.07999740272964573 1103 54-55 -0.3191781147515371 1103 56-57 -0.3163920370801456 1103 58-59 0.08033643739172902 1103 60-61 0.08186070295085912 1103 62-63 0.10309993220964486 1103 64-65 -0.19207187755807453 1103 66-67 -0.25285286138140606 1103 68-69 -0.32688910790908565 1103 70-71 -0.5052126872619631 1103 72-73 -0.43951690767360674 1103 74-75 -0.12517995951699845 1103 76-77 -0.06269009001074011 1103 78-79 -0.32164835806785774 1103 80-81 -0.20353118829091343 1103 82-83 -0.6251154974533151 1103 84-85 -0.25094338655407356 1103 86-87 -0.22483441943246518 1103 88-89 -0.7193967258584166 1103 90-91 -1.2077489997669986 1103 92-93 -0.9315533813244343 1103 94-95 -0.8878680962547705 1103 96-97 -0.6558936012352063 1103 98-99 -0.6544647865567335 1103 100-101 0.2376187840593893 1104 1 0.14380998305485448 1104 2 0.17286844866634254 1104 3 0.11008431958370579 1104 4 0.15265378459685763 1104 5 0.048667113297071296 1104 6 0.044998254205104615 1104 7 -0.06201542993802889 1104 8 -0.00286876916954526 1104 9 0.19723068375314767 1104 10-11 0.20890949825400185 1104 12-13 0.15881960496664504 1104 14-15 0.07645792237786253 1104 16-17 0.24006795205771425 1104 18-19 0.22020181145727236 1104 20-21 0.01944046815064837 1104 22-23 -0.05915703008698614 1104 24-25 0.1765067930877109 1104 26-27 0.2337566472860928 1104 28-29 0.1088215792939522 1104 30-31 0.06586550368118793 1104 32-33 0.3010288943215116 1104 34-35 0.17353531389480992 1104 36-37 0.537199849136023 1104 38-39 0.6141484490254854 1104 40-41 0.4082184227458825 1104 42-43 0.778507553765877 1104 44-45 1.1311162460847228 1104 46-47 0.9122617192113154 1104 48-49 1.1639905286539687 1104 50-51 1.0549501156064665 1104 52-53 0.7317241605691294 1104 54-55 0.7069753594852344 1104 56-57 0.5286625284675814 1104 58-59 0.5030640240749698 1104 60-61 0.4980335168754344 1104 62-63 0.6785488892479101 1104 64-65 0.6219528496141891 1104 66-67 0.5597593778781622 1104 68-69 0.8453273584536731 1104 70-71 0.8126385352825878 1104 72-73 0.735130833727137 1104 74-75 0.8647392282161128 1104 76-77 0.7923589499119004 1104 78-79 0.8427493487977316 1104 80-81 0.8335664771324893 1104 82-83 0.36524228677102144 1104 84-85 0.4644523863427388 1104 86-87 0.7350944381445466 1104 88-89 0.7784862690303811 1104 90-91 0.854611132848003 1104 92-93 0.7847195845548285 1104 94-95 0.8841342521358904 1104 96-97 0.9506508109764233 1104 98-99 1.0594148929570117 1104 100-101 1.1055736120743127 1105 1 -0.017342899150659008 1105 2 0.11571166782535158 1105 3 -0.029180509154805634 1105 4 0.22892780297613768 1105 5 0.04738613306872708 1105 6 0.04139201638834322 1105 7 -0.0428981923997398 1105 8 0.10141972985579883 1105 9 0.2711515971477425 1105 10-11 0.09868950382348629 1105 12-13 0.009543637269622707 1105 14-15 -6.097467950709756E-4 1105 16-17 -0.11796602176573145 1105 18-19 -0.20813987609493978 1105 20-21 -0.01587003031774259 1105 22-23 -0.0970573364083478 1105 24-25 0.13907989250291308 1105 26-27 -0.056608153605026246 1105 28-29 0.027959702379448004 1105 30-31 0.12959427004152957 1105 32-33 0.39231265928948744 1105 34-35 0.35910340077868597 1105 36-37 0.2930129931070624 1105 38-39 0.36584017834879035 1105 40-41 0.19510926095250625 1105 42-43 0.5972697370017457 1105 44-45 0.3484790976580996 1105 46-47 0.5069498283731093 1105 48-49 0.803123082260214 1105 50-51 0.6210319869515004 1105 52-53 0.6791204290180275 1105 54-55 0.706787389560418 1105 56-57 0.7771587690690858 1105 58-59 0.7004672543729384 1105 60-61 0.6372496126704768 1105 62-63 0.5647713899440951 1105 64-65 0.6041931726439813 1105 66-67 0.4386232041104101 1105 68-69 0.4200071133965828 1105 70-71 0.8726984071845649 1105 72-73 0.8345530002545658 1105 74-75 0.783571029942653 1105 76-77 0.5649710356818822 1105 78-79 0.4704505462357709 1105 80-81 0.4612607127214581 1105 82-83 0.8894555978264407 1105 84-85 0.7028957168913301 1105 86-87 0.6331520822548207 1105 88-89 0.3826842640178505 1105 90-91 0.39037415150575683 1105 92-93 0.5050899549252001 1105 94-95 0.858382372436644 1105 96-97 0.6402985414136282 1105 98-99 0.7392464162095855 1105 100-101 1.4372709108768724 1106 1 0.09424203332979886 1106 2 -0.09166415149076457 1106 3 -0.009161556300028906 1106 4 0.060247395489760436 1106 5 -0.1992773747888137 1106 6 0.12458715182228275 1106 7 0.0561699589072191 1106 8 0.06521188021510937 1106 9 0.14160093774633964 1106 10-11 0.22456804761247184 1106 12-13 0.26501232557412635 1106 14-15 0.3213518736740113 1106 16-17 0.37241935294800044 1106 18-19 0.12454195285554448 1106 20-21 0.055064977184429154 1106 22-23 0.05798096415234255 1106 24-25 0.08103677475831006 1106 26-27 0.1562363540155971 1106 28-29 0.2238058683748747 1106 30-31 0.14550677102865706 1106 32-33 -0.18792109258605194 1106 34-35 -0.012017186759813114 1106 36-37 -0.26297689870371954 1106 38-39 -0.18941923885092393 1106 40-41 0.2268947778126531 1106 42-43 -0.19640079920610276 1106 44-45 -0.21538807441802987 1106 46-47 -0.1019501163477301 1106 48-49 -0.42186309794985277 1106 50-51 -0.3250708322856468 1106 52-53 -0.27144420811377756 1106 54-55 -0.6691898670138485 1106 56-57 -0.8500622685997143 1106 58-59 -0.6444549599522595 1106 60-61 -0.4207737625170793 1106 62-63 -0.2988470259131297 1106 64-65 -0.7453488000323176 1106 66-67 -0.6064490536484151 1106 68-69 -0.5937731914284932 1106 70-71 -0.4478812342906018 1106 72-73 -0.5879969484147836 1106 74-75 -0.4835260244678352 1106 76-77 -0.68157401683332 1106 78-79 -1.0465083102753248 1106 80-81 -1.0046241486857888 1106 82-83 -0.8031133036976854 1106 84-85 -0.833924152018092 1106 86-87 -0.5127256718318876 1106 88-89 -0.5318502064867658 1106 90-91 -0.6297237715772397 1106 92-93 -0.46105155972377787 1106 94-95 -0.3470191911738745 1106 96-97 -0.160183176978542 1106 98-99 0.031456003195291515 1106 100-101 0.4620478199821427 1107 1 0.001704719896959972 1107 2 0.17300002761371047 1107 3 -0.05636304883734056 1107 4 0.04436431091264126 1107 5 -0.04672762354503135 1107 6 0.024077201573525997 1107 7 0.14982667532513005 1107 8 -0.06089508495901441 1107 9 -0.061321947825803136 1107 10-11 0.11548844562242522 1107 12-13 0.18447749970348326 1107 14-15 0.10599739606207237 1107 16-17 0.059739004689291164 1107 18-19 0.1280307588256946 1107 20-21 0.025624678676962276 1107 22-23 0.27650086464985435 1107 24-25 0.05644100361402593 1107 26-27 0.20520401570714597 1107 28-29 0.020466316136058538 1107 30-31 0.08862866157592464 1107 32-33 -0.04660268462585293 1107 34-35 -0.15758310715782642 1107 36-37 -0.25773436139029116 1107 38-39 0.4794116069202232 1107 40-41 0.339600001693249 1107 42-43 0.37245492218693244 1107 44-45 0.21453729871630145 1107 46-47 0.2737090876323691 1107 48-49 0.2204378970750227 1107 50-51 0.09422643139594555 1107 52-53 0.10757942372702445 1107 54-55 0.1550016752747112 1107 56-57 0.12287305478337274 1107 58-59 0.45135349775918243 1107 60-61 0.24369141161227503 1107 62-63 0.4699962576689636 1107 64-65 0.41853179698260945 1107 66-67 0.6757462199834237 1107 68-69 0.4758536742431474 1107 70-71 0.7613227458089007 1107 72-73 0.6105913600429247 1107 74-75 0.6955287019003258 1107 76-77 0.6624247393855853 1107 78-79 0.5009730330082576 1107 80-81 0.7368559508167003 1107 82-83 0.8801764972973416 1107 84-85 1.0136629126585284 1107 86-87 1.0446997013024415 1107 88-89 0.9825652163988039 1107 90-91 1.134150606532213 1107 92-93 1.54096958455483 1107 94-95 1.0937395152937874 1107 96-97 1.2247297583448429 1107 98-99 1.0837569982201671 1107 100-101 1.4160341383900992 1108 1 0.2597506969084584 1108 2 -0.06472985744375848 1108 3 0.12366568679484402 1108 4 -0.07922764311034314 1108 5 -0.036813830441587925 1108 6 -0.12112394785176406 1108 7 0.09494161785386268 1108 8 0.025110662167421083 1108 9 0.0020401211397143015 1108 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2316 62-63 -1.770966945794239 2316 64-65 -0.9327236142728026 2316 66-67 -0.6905254250382171 2316 68-69 -0.93689524350577 2316 70-71 -0.8168157823296234 2316 72-73 -0.7515460858445202 2316 74-75 -0.821527575155951 2316 76-77 -0.7746153039044579 2316 78-79 -0.6986211228358989 2316 80-81 -0.8168778154170688 2316 82-83 -0.9805540221831777 2316 84-85 -1.1392158752202626 2316 86-87 -1.2867613376586 2316 88-89 -1.339177207843619 2316 90-91 -1.6535171423855353 2316 92-93 -1.5893063894711474 2316 94-95 -1.34832758427331 2316 96-97 -1.1514282503131668 2316 98-99 -0.9882668113036424 2316 100-101 -0.6880297144237524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 15.0 5 46.0 6 182.0 7 333.0 8 538.0 9 881.0 10 1433.0 11 2271.0 12 3222.0 13 4385.0 14 4759.0 15 6053.0 16 7505.0 17 9463.0 18 8492.0 19 5374.0 20 4725.0 21 5001.0 22 4938.0 23 5133.0 24 5508.0 25 6052.0 26 6181.0 27 6687.0 28 7717.0 29 8940.0 30 10406.0 31 12230.0 32 14730.0 33 17840.0 34 24014.0 35 36536.0 36 41192.0 37 59309.0 38 49499.0 39 4671.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.41766374225758 20.759931184268908 15.36573277595705 17.456672297516455 2 15.11246194312698 25.06601702462564 39.10045978915975 20.72106124308763 3 20.25143425222128 31.37905681088582 31.880786198040717 16.488722738852186 4 11.59233063397055 20.716660108112585 36.64177867986662 31.049230578050246 5 10.629471672609476 42.4844826648644 32.46638234403039 14.419663318495731 6 24.31326149655919 38.35172403079902 19.30531319418195 18.029701278459843 7 22.376146883996437 39.531771016713954 21.004028332953627 17.088053766335978 8 24.58706998322391 33.81961559969348 20.85904976906986 20.734264648012758 9 26.299629269100926 18.977694012385314 22.210457096700704 32.51221962181306 10-11 22.67218793364124 29.30081498664126 26.58893917113684 21.43805790858066 12-13 27.690905701799807 28.044420396412818 27.092480790340286 17.172193111447093 14-15 23.47552451173291 28.8990172524491 29.697305469834102 17.92815276598389 16-17 17.33976093265778 32.31292605067689 31.158624832039273 19.18868818462606 18-19 16.850910258268957 30.02557836091378 35.92322867261769 17.200282708199573 20-21 18.96682062009403 28.429519706729078 34.69065198931301 17.913007683863885 22-23 17.906923761986622 29.533298469440588 34.135073421287 18.424704347285793 24-25 18.195268761528755 29.79088055402738 35.2844244522831 16.729426232160773 26-27 18.58111300534225 28.866140855717838 35.9383110620941 16.61443507684581 28-29 16.537394113870306 29.371880371973575 36.94416254168134 17.146562972474786 30-31 16.59888055837458 30.928199366236562 36.014875836215644 16.45804423917321 32-33 16.22750243356875 31.1732390282294 35.37528736822484 17.22397116997701 34-35 16.862899937213985 30.418983384360455 35.48833927750772 17.229777400917843 36-37 17.72761229296056 30.271486592687253 35.154076312474345 16.846824801877837 38-39 18.423233076476926 29.2808538404176 35.501724459358684 16.794188623746788 40-41 16.930503879882867 30.015172581643245 36.437757461363 16.616566077110893 42-43 16.52912033782673 31.376874351914065 34.98168853815115 17.112316772108063 44-45 16.289666563970062 31.653639731677067 33.63641776979218 18.42027593456069 46-47 17.045964974505615 30.675073751849457 32.34893977266696 19.930021500977965 48-49 17.575208408843785 30.68270077150106 32.30298244705639 19.439108372598767 50-51 17.701905564465708 31.433593906750186 32.34268834616783 18.52181218261627 52-53 16.491829422363978 31.63833543897438 32.890717229666755 18.979117908994883 54-55 15.796708986599839 31.294788020628378 32.57694219497546 20.331560797796325 56-57 17.155354422137677 31.92834794585449 30.400473261869408 20.515824370138418 58-59 16.809401884208427 31.007111734750676 31.280241700602957 20.90324468043794 60-61 17.162477912764317 30.568734182950273 31.50672163934214 20.762066264943268 62-63 18.082509190700563 30.533060632734426 31.2639154973334 20.120514679231604 64-65 17.39849143259536 30.933916359555276 31.681986638550924 19.98560556929844 66-67 16.546965617232807 31.063975766362883 31.608455882352942 20.780602734051367 68-69 16.319643557538093 31.206190624745155 31.53343007263214 20.940735745084613 70-71 16.69622330841083 30.618307685935008 30.584004722158937 22.101464283495226 72-73 16.31656069424021 29.911796077294284 30.506855414949364 23.264787813516143 74-75 16.101314258271877 30.04063288402865 31.127326294097163 22.730726563602307 76-77 16.433828196035055 28.885095315685444 32.09984638618978 22.581230102089716 78-79 15.620345609366272 28.73449832717393 32.62859890932518 23.016557154134624 80-81 15.645072347828789 29.742996279698335 31.611690601384048 23.00024077108883 82-83 15.427043960026927 29.215683736343394 31.239709004297623 24.117563299332055 84-85 15.658073234885986 28.130501418718808 31.303978626017436 24.907446720377774 86-87 15.794302300719826 28.245401475933473 31.259208386515745 24.701087836830954 88-89 16.27942322681216 27.203299558326837 31.526240581969343 24.99103663289166 90-91 15.424721086932507 27.366683777233412 31.912983021552815 25.29561211428126 92-93 15.1016080991344 27.89779515796859 31.330684882211234 25.669911860685772 94-95 15.009313590334058 27.538325521308643 30.926556612260804 26.525804276096498 96-97 14.910935151781102 26.7743392787566 30.707017359911042 27.60770820955125 98-99 15.214205828138269 26.573146863285213 30.891825280119296 27.32082202845722 100-101 15.575798135709285 26.186799289078973 31.53437002843943 26.703032546772317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4564.0 1 2996.0 2 1797.5 3 2108.0 4 1700.5 5 1516.0 6 2347.5 7 3942.5 8 5007.0 9 4852.5 10 3584.5 11 2401.5 12 2191.5 13 2091.5 14 1925.0 15 1723.5 16 1539.0 17 1517.0 18 1541.5 19 1530.0 20 1605.5 21 1754.0 22 1927.5 23 2160.0 24 2409.5 25 2734.5 26 3202.0 27 3793.5 28 4407.0 29 5069.5 30 5756.0 31 6504.0 32 7411.0 33 8346.0 34 9454.0 35 10486.0 36 11330.5 37 11870.5 38 11623.5 39 10930.5 40 10300.5 41 9986.5 42 9916.5 43 9909.0 44 10233.5 45 10837.0 46 12353.5 47 15632.5 48 21169.5 49 20648.0 50 15625.0 51 12608.0 52 9000.5 53 6701.5 54 5748.0 55 5223.5 56 4696.5 57 4109.0 58 3654.5 59 3357.0 60 2927.5 61 2498.0 62 2066.5 63 1443.5 64 952.0 65 709.5 66 528.5 67 405.0 68 308.5 69 247.5 70 221.0 71 176.5 72 137.5 73 139.0 74 125.0 75 81.0 76 57.0 77 40.0 78 28.5 79 24.0 80 20.5 81 17.0 82 12.0 83 6.5 84 4.5 85 4.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2296356895801835 2 0.0 3 0.0 4 0.0 5 0.021487894289915706 6 0.0046600252676925625 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0033655738044446287 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.883354389743802E-4 26-27 9.061160242735539E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008155044218461985 36-37 0.019546217095043804 38-39 0.014497856388376862 40-41 0.010614501998633058 42-43 0.007896153925812397 44-45 0.0033655738044446287 46-47 6.47225731623967E-4 48-49 0.0010355611705983472 50-51 1.294451463247934E-4 52-53 0.0 54-55 0.0 56-57 0.002200567487521488 58-59 2.588902926495868E-4 60-61 0.0029772383654702484 62-63 0.0010355611705983472 64-65 9.061160242735539E-4 66-67 0.006213367023590083 68-69 6.47225731623967E-4 70-71 0.0 72-73 0.0 74-75 1.294451463247934E-4 76-77 0.059803657602054555 78-79 0.1396713128844521 80-81 0.001294451463247934 82-83 0.0010355611705983472 84-85 0.0 86-87 0.10523890396205704 88-89 0.3531263591740364 90-91 0.3913126773398504 92-93 1.294451463247934E-4 94-95 1.294451463247934E-4 96-97 1.294451463247934E-4 98-99 0.0 100-101 0.0011650063169231406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 386264.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.80673457159429 #Duplication Level Percentage of deduplicated Percentage of total 1 88.59424485848373 53.871267316806524 2 7.410562728869534 9.012242417610384 3 1.599204376997892 2.917271882335279 4 0.6924877104448712 1.6843166561244929 5 0.3911802816278193 1.1893197777292155 6 0.23685724042897663 0.8641509210075045 7 0.15798448814419208 0.672456458590915 8 0.1290559496688469 0.6277976701118884 9 0.0905558972108595 0.4955767565033978 >10 0.593892950524707 6.73865439195601 >50 0.04474270224531125 1.8837315136544945 >100 0.046447186140330526 5.665445604680628 >500 0.005965693632703003 2.513628530116935 >1k 0.005539572658938502 6.809935576593473 >5k 0.0012783629212935006 5.05420452617884 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7078 1.8324254913737754 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6454 1.6708779487604333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 1.5466106082886317 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4708 1.2188554977942547 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2915 0.7546652030735456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2793 0.723080587370296 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2624 0.6793281279125157 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2167 0.5610152641716546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.5128616697388314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1733 0.4486568771617339 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1732 0.4483979868690844 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.32542509786053064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1188 0.30756166766770915 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1084 0.2806370772321521 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1071 0.2772715034277075 RNA PCR Primer, Index 39 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.2663981111364248 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 911 0.2358490566037736 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 878 0.22730567694633722 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 848 0.21953896816684962 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 779 0.20167553797402812 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 720 0.1864010107077025 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 713 0.18458877865915538 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 696 0.18018764368411241 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 667 0.1726798251972744 RNA PCR Primer, Index 39 (96% over 30bp) GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 641 0.16594867758838514 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 610 0.15792307851624796 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 595 0.15403972412650416 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 587 0.15196860178530747 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.14005964832342646 No Hit ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 515 0.1333285007145372 RNA PCR Primer, Index 39 (96% over 25bp) ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 437 0.11313505788786943 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 435 0.11261727730257026 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 432 0.11184060642462151 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 401 0.10381500735248432 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 395 0.10226166559658678 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 394 0.10200277530393721 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.588902926495868E-4 0.0 18-19 0.0 0.0 0.0 0.0010355611705983472 0.0 20-21 0.0 0.0 0.0 0.003106683511795042 0.0 22-23 0.0 0.0 0.0 0.008025599072137192 0.0 24-25 0.0 0.0 0.0 0.015662862705300002 0.0 26-27 0.0 0.0 0.0 0.03223184143487356 0.0 28-29 0.0 0.0 0.0 0.08478657084273968 0.0 30-31 0.0 0.0 0.0 0.18562433982975374 0.0 32-33 0.0 0.0 0.0 0.32697843961642814 0.0 34-35 0.0 0.0 0.0 0.5737008885114844 0.0 36-37 0.0 0.0 0.0 0.9151771845162894 0.0 38-39 0.0 0.0 0.0 1.3438995091440051 0.0 40-41 0.0 0.0 0.0 1.8159859577905269 0.0 42-43 0.0 0.0 0.0 2.213900337592942 0.0 44-45 0.0 0.0 0.0 2.6204875421991174 0.0 46-47 0.0 0.0 0.0 3.0945156680405113 0.0 48-49 0.0 0.0 0.0 3.584983327465153 0.0 50-51 0.0 0.0 0.0 4.108977279787917 0.0 52-53 0.0 0.0 0.0 4.602939958163329 0.0 54-55 0.0 0.0 0.0 5.089006482612928 0.0 56-57 0.0 0.0 0.0 5.61028208686287 0.0 58-59 1.294451463247934E-4 0.0 0.0 6.245598865024958 0.0 60-61 2.588902926495868E-4 0.0 0.0 6.98913178551457 0.0 62-63 2.588902926495868E-4 0.0 0.0 7.643994780771701 0.0 64-65 2.588902926495868E-4 0.0 0.0 8.234523538305407 0.0 66-67 2.588902926495868E-4 0.0 0.0 8.885244288880145 0.0 68-69 2.588902926495868E-4 0.0 0.0 9.606512644201892 0.0 70-71 2.588902926495868E-4 0.0 0.0 10.40984922229356 0.0 72-73 2.588902926495868E-4 0.0 0.0 11.241145951991385 0.0 74-75 2.588902926495868E-4 0.0 0.0 12.052637574301514 0.0 76-77 2.588902926495868E-4 0.0 0.0 12.832932916347367 0.0 78-79 2.588902926495868E-4 0.0 0.0 13.680798624774766 0.0 80-81 2.588902926495868E-4 0.0 0.0 14.604001408363192 0.0 82-83 2.588902926495868E-4 0.0 0.0 15.537430358511278 0.0 84-85 2.588902926495868E-4 0.0 0.0 16.58619493403475 0.0 86-87 2.588902926495868E-4 0.0 0.0 17.627840026510366 0.0 88-89 2.588902926495868E-4 0.0 0.0 18.553761158171614 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCGGA 20 0.0020601607 71.36193 1 GTCATAG 70 2.0319864E-4 33.98187 1 CTACTCG 35 0.00818974 27.277529 84-85 CATGGGT 320 0.0 25.211853 4 ATGGGAG 780 0.0 24.945665 5 CATGGGG 1150 0.0 24.347815 4 GGGACTC 300 0.0 23.728804 7 ATGGGTT 100 0.0016602715 23.728802 5 TAGACTG 100 0.0016602715 23.728802 5 TACAAGG 100 0.0016602715 23.728802 2 GACTAAG 120 1.7183242E-4 23.728802 7 GTACATG 5550 0.0 23.230127 1 GTCCTAC 270 4.0017767E-11 22.906298 1 TACATGG 5590 0.0 22.83738 2 CCTCTAG 125 2.2283159E-4 22.835817 1 GGACTCC 335 0.0 22.666319 8 ATGGGAC 440 0.0 22.65022 5 CTACTTG 105 0.0022050138 22.59886 9 ACATGGG 5645 0.0 22.110453 3 GTAGGAC 280 6.730261E-11 22.033888 3 >>END_MODULE