FastQCFastQC Report
Thu 26 May 2016
SRR938807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435823
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45371.0410189457646797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42670.97906719012076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.856769835460726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.5598603102635703No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.537603568421125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10830.24849537541616665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.23908788659616403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.20191683320981224No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7660.1757594252712684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.16061566278053246No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.1523554286946765No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4830.11082480731856739No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGG250.005067643756.8617821
GCGAGAT500.00164705237.846927
TTAGCGA550.0026249734.4181444
TAGCGAG550.0026249734.4181445
ATTAGCG650.00594644929.1230473
GGATATG1703.7234713E-927.8734231
CGCAGAA1151.3145019E-424.6856089
GGTATCA40250.024.3693351
ATACTCA1000.00169240323.6516086
ATGGGAT5350.022.999045
ACCCATG2154.5813067E-822.0116043
CATATAG1303.0116958E-421.8422853
GAGTACT28750.021.72582812-13
GTACATG26300.021.6204511
CATTCCG1100.002940686421.50649
CCGACTA1100.002940686421.50649
CTAGACC1353.8864068E-421.0333124
GGCCGAC1353.8954604E-421.0260667
TACATGG26800.020.8371052
TACCCAT2309.345422E-820.5760652
Produced by FastQC (version 0.11.5)