##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938807_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 435823 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.534402727712855 33.0 31.0 34.0 30.0 34.0 2 31.578404535786316 33.0 31.0 34.0 30.0 34.0 3 31.550168302269498 33.0 31.0 34.0 28.0 34.0 4 35.11972750405555 37.0 35.0 37.0 33.0 37.0 5 35.16995202180702 37.0 35.0 37.0 33.0 37.0 6 35.27562794987873 37.0 35.0 37.0 33.0 37.0 7 35.215319063014114 37.0 35.0 37.0 33.0 37.0 8 35.205298940166074 37.0 35.0 37.0 32.0 37.0 9 36.74678252409809 39.0 37.0 39.0 33.0 39.0 10-11 36.67497929205204 39.0 37.0 39.0 32.0 39.0 12-13 36.58283408631486 39.0 37.0 39.0 32.0 39.0 14-15 37.69121753555916 40.0 37.5 41.0 32.0 41.0 16-17 37.62494407133171 40.0 37.0 41.0 32.0 41.0 18-19 37.61424477368106 40.0 37.0 41.0 32.0 41.0 20-21 37.54305188115359 40.0 37.0 41.0 32.0 41.0 22-23 37.41626417146411 40.0 37.0 41.0 32.0 41.0 24-25 37.37185967697896 40.0 37.0 41.0 32.0 41.0 26-27 37.250617796674334 40.0 36.5 41.0 32.0 41.0 28-29 37.11033951856602 40.0 36.0 41.0 31.5 41.0 30-31 36.84258632518247 39.0 36.0 41.0 31.0 41.0 32-33 36.53675689442733 39.0 35.0 41.0 30.5 41.0 34-35 36.17535788611431 39.0 35.0 41.0 30.0 41.0 36-37 35.89696964134522 39.0 35.0 41.0 30.0 41.0 38-39 35.69626086737047 39.0 35.0 41.0 29.0 41.0 40-41 35.48126991921032 39.0 35.0 41.0 27.0 41.0 42-43 35.16434997694018 38.0 35.0 40.0 25.5 41.0 44-45 34.931958616227234 39.0 35.0 41.0 24.0 41.0 46-47 34.60629085660922 39.0 35.0 41.0 22.5 41.0 48-49 34.28102693065763 39.0 34.5 41.0 19.0 41.0 50-51 33.134633784816316 38.0 32.5 40.0 14.5 40.5 52-53 33.23372447071402 38.0 33.0 39.5 13.0 40.5 54-55 33.760782473618875 38.0 34.0 40.0 10.5 41.0 56-57 33.689734135187905 38.0 34.0 40.5 8.0 41.0 58-59 33.497315423922096 38.0 34.0 40.0 6.5 41.0 60-61 33.246134325173294 38.0 33.5 40.0 2.0 41.0 62-63 32.91455820367443 38.0 33.0 40.0 2.0 41.0 64-65 32.550691450428275 37.0 33.0 40.0 2.0 41.0 66-67 32.16065237493203 36.5 32.0 39.5 2.0 41.0 68-69 31.74820626722316 36.0 32.0 39.0 2.0 41.0 70-71 31.344233782980705 36.0 31.0 39.0 2.0 40.5 72-73 30.90379695426813 35.0 31.0 38.5 2.0 40.0 74-75 30.415630886850856 35.0 30.5 37.5 2.0 40.0 76-77 29.937412665233364 35.0 30.0 37.0 2.0 39.0 78-79 29.45975774569034 35.0 29.5 36.5 2.0 39.0 80-81 28.95877340112844 34.5 29.0 36.0 2.0 38.5 82-83 28.43437129293314 34.0 28.5 35.5 2.0 37.0 84-85 27.940721347886644 34.0 27.5 35.0 2.0 37.0 86-87 27.511021676230946 34.0 26.5 35.0 2.0 36.0 88-89 27.0760320129961 34.0 26.0 35.0 2.0 36.0 90-91 26.615954183234937 34.0 25.0 35.0 2.0 36.0 92-93 26.12739804920805 34.0 23.5 35.0 2.0 35.0 94-95 25.585839893718322 33.5 19.0 35.0 2.0 35.0 96-97 24.99325299490848 33.0 7.5 35.0 2.0 35.0 98-99 24.339587401307412 33.0 2.0 35.0 2.0 35.0 100-101 22.35781154275933 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49252523056014397 1101 2 0.18367201815249956 1101 3 0.1545749479310139 1101 4 0.3869130449501057 1101 5 0.06406051188003659 1101 6 0.10765861753530004 1101 7 0.32985140888846587 1101 8 0.3739214537329474 1101 9 0.738168750550166 1101 10-11 0.39387529631283513 1101 12-13 0.24460551538456343 1101 14-15 0.3590233725171643 1101 16-17 0.5715385867367928 1101 18-19 0.5947667199290123 1101 20-21 0.6383360761368024 1101 22-23 0.5177463017255093 1101 24-25 0.6973803638489358 1101 26-27 0.6051698972419359 1101 28-29 0.6145060228372685 1101 30-31 0.38627298712230385 1101 32-33 0.4663815238333129 1101 34-35 0.46012299971970094 1101 36-37 0.5137606500698553 1101 38-39 0.5371043259747807 1101 40-41 0.5804979148988565 1101 42-43 0.28392556136654434 1101 44-45 0.3260732409083573 1101 46-47 0.4774480052143346 1101 48-49 0.2786931737947498 1101 50-51 -0.06380311806605476 1101 52-53 0.12344265801617382 1101 54-55 0.22448589501272664 1101 56-57 0.2772365170489479 1101 58-59 0.37955362884099486 1101 60-61 0.15169275953443417 1101 62-63 -0.002069985792978457 1101 64-65 0.2056003333201062 1101 66-67 -0.011461811174591219 1101 68-69 -0.05413884481194131 1101 70-71 -0.20031780036836722 1101 72-73 -0.3464184489908355 1101 74-75 -0.6218517810684254 1101 76-77 -0.6823910515160847 1101 78-79 -0.471122031266912 1101 80-81 -0.5711877536672638 1101 82-83 -0.785007027505646 1101 84-85 -0.5648601612995314 1101 86-87 -0.544417082519228 1101 88-89 -0.39337781819431683 1101 90-91 -0.4853930320278046 1101 92-93 -0.7977539073158901 1101 94-95 -0.9440929810212033 1101 96-97 -0.8047448891668552 1101 98-99 -0.7593237175071046 1101 100-101 -0.5488089374851022 1102 1 0.1798748546202944 1102 2 0.16422339659861507 1102 3 0.28772657700369564 1102 4 0.33310352114057906 1102 5 0.25842767979983705 1102 6 0.33163104861299075 1102 7 0.23842283745989334 1102 8 0.07243022566277091 1102 9 0.4218654924047982 1102 10-11 0.21740599806721406 1102 12-13 0.4550096507229071 1102 14-15 0.1812633474545038 1102 16-17 0.3234214188169915 1102 18-19 0.5704152161696143 1102 20-21 0.6153003618510837 1102 22-23 0.527821489695441 1102 24-25 0.4871485342750006 1102 26-27 0.651748844610367 1102 28-29 0.5851231907570664 1102 30-31 0.6343306312325794 1102 32-33 0.4899654837330658 1102 34-35 0.6408936764114372 1102 36-37 0.8451672916738602 1102 38-39 0.6139558297341807 1102 40-41 0.5289440301870769 1102 42-43 0.5876536315419827 1102 44-45 0.4003934163469509 1102 46-47 0.17712532351007582 1102 48-49 0.0671768830679369 1102 50-51 0.3334023957184016 1102 52-53 0.17493075325426588 1102 54-55 0.0819764965164822 1102 56-57 0.11967699323943037 1102 58-59 0.06099159876580984 1102 60-61 0.16436819813092995 1102 62-63 0.2630271320015112 1102 64-65 0.3098797819416603 1102 66-67 0.2743433266700279 1102 68-69 -0.14587130095229028 1102 70-71 -0.05818434172174847 1102 72-73 0.15723130038259825 1102 74-75 -0.0025190743015102157 1102 76-77 0.2245419058773983 1102 78-79 0.05150954768045679 1102 80-81 -0.12585254063467843 1102 82-83 0.18967968928633283 1102 84-85 0.0680063800538484 1102 86-87 0.22449582474894214 1102 88-89 0.029977444963577682 1102 90-91 0.11058127874913737 1102 92-93 0.2402316816565424 1102 94-95 0.08434686860285723 1102 96-97 -0.2627336109713667 1102 98-99 -0.2095994067301632 1102 100-101 0.021582666524924576 1103 1 0.30034551476699534 1103 2 0.3333187511218405 1103 3 0.42256154032888205 1103 4 0.39366586832884565 1103 5 0.057871445081012496 1103 6 0.30298801682815224 1103 7 0.1887406858706555 1103 8 0.36911726722755844 1103 9 0.48336915988646467 1103 10-11 0.408845484619782 1103 12-13 0.6201960810407527 1103 14-15 0.564304300999737 1103 16-17 0.6144024701617354 1103 18-19 0.7245808640913722 1103 20-21 0.7125589193083002 1103 22-23 0.9377970398176814 1103 24-25 0.7366778741283042 1103 26-27 0.8761131477888426 1103 28-29 0.7880235575052339 1103 30-31 0.7450274527484737 1103 32-33 0.7911635277428459 1103 34-35 0.8808631140642476 1103 36-37 0.6534344065882891 1103 38-39 0.49164226005202494 1103 40-41 0.5921958639279055 1103 42-43 0.26781866821679756 1103 44-45 0.36832434544231063 1103 46-47 0.3361503846347702 1103 48-49 0.37755585617306764 1103 50-51 0.6130234226132671 1103 52-53 0.31892525203177513 1103 54-55 0.4175480124089077 1103 56-57 0.47347589299492654 1103 58-59 0.4486291293281539 1103 60-61 0.4895943595001242 1103 62-63 0.6162392346910011 1103 64-65 0.49806437851867713 1103 66-67 0.4792974831492387 1103 68-69 0.47659508046580257 1103 70-71 0.23619524263033043 1103 72-73 0.22839572581047207 1103 74-75 0.2224778694637699 1103 76-77 0.32551073228326644 1103 78-79 0.42625282396407727 1103 80-81 -0.1608189220527727 1103 82-83 0.14591440811273415 1103 84-85 0.030411636777568418 1103 86-87 -0.27632018992098395 1103 88-89 -0.24687157704131124 1103 90-91 -0.11278363567629057 1103 92-93 0.05785087481057616 1103 94-95 -0.3676641338421298 1103 96-97 0.1599130882951414 1103 98-99 8.162411915968448E-4 1103 100-101 0.13839501371318974 1104 1 0.17223165652960404 1104 2 -0.020597605788022832 1104 3 0.12883039561944898 1104 4 0.09699373593771554 1104 5 0.145056558081464 1104 6 0.08087925863686252 1104 7 0.24052307612338097 1104 8 0.11394573878926195 1104 9 0.11662682892031029 1104 10-11 0.12477174985719586 1104 12-13 0.22368208509045218 1104 14-15 0.1118152567623838 1104 16-17 0.3819506550938385 1104 18-19 0.28495280566841785 1104 20-21 0.33216492032364187 1104 22-23 0.5394682677383997 1104 24-25 0.4137177466855064 1104 26-27 0.3966533792165663 1104 28-29 0.29693404994561234 1104 30-31 0.35754065509892996 1104 32-33 0.2949535505588372 1104 34-35 0.44261802008685436 1104 36-37 0.3365604897645511 1104 38-39 0.5235292187556624 1104 40-41 0.45417076049734106 1104 42-43 0.4469913403725343 1104 44-45 0.6830216979698633 1104 46-47 0.7684230562069772 1104 48-49 0.09222461576483454 1104 50-51 0.29453604726970894 1104 52-53 0.4859838558795673 1104 54-55 0.6932801958004902 1104 56-57 0.3441191173444409 1104 58-59 0.39268909279921616 1104 60-61 0.47127153941971045 1104 62-63 0.40909216780103463 1104 64-65 0.4436327652352219 1104 66-67 0.4633379567416185 1104 68-69 0.21111258926250542 1104 70-71 -0.06889138229454161 1104 72-73 0.0025027800962043045 1104 74-75 -0.5391270695282415 1104 76-77 -0.33880236619897275 1104 78-79 -0.383884247068945 1104 80-81 -0.5659808222575897 1104 82-83 -0.1656902391146211 1104 84-85 -0.3203915913065316 1104 86-87 0.09353818751743859 1104 88-89 0.09669880534544006 1104 90-91 -0.07060905537974094 1104 92-93 0.03371318523649691 1104 94-95 0.12874424330452783 1104 96-97 0.5248260548997585 1104 98-99 0.24018579613379742 1104 100-101 0.7939901844246862 1105 1 0.1980753377120834 1105 2 0.18924755118798586 1105 3 0.23648261565103823 1105 4 0.32687163708261124 1105 5 0.265033959993076 1105 6 0.34155858484487567 1105 7 0.3188093108898471 1105 8 0.3426476169671204 1105 9 0.33039206245310737 1105 10-11 0.387073872463354 1105 12-13 0.30239491642338834 1105 14-15 0.23810513489411989 1105 16-17 0.10848784393776612 1105 18-19 0.3227642499860366 1105 20-21 0.4385431154742747 1105 22-23 0.33888429162780653 1105 24-25 0.3631026405551978 1105 26-27 0.252449327702152 1105 28-29 0.4966388912401598 1105 30-31 0.3677847375127783 1105 32-33 0.4120427784190568 1105 34-35 0.4169444010491148 1105 36-37 0.6005718810458447 1105 38-39 0.4552420616182431 1105 40-41 0.46739813646727413 1105 42-43 0.8258782692231392 1105 44-45 0.3790226434000985 1105 46-47 0.0887134877612823 1105 48-49 -0.0959390589610507 1105 50-51 -0.3398101646680729 1105 52-53 -0.13276852210805146 1105 54-55 -0.06550538754148505 1105 56-57 -0.16082421449004158 1105 58-59 -0.2155482563066613 1105 60-61 -0.18513663278694992 1105 62-63 -0.6492132061627345 1105 64-65 -0.4558086238554395 1105 66-67 -0.4708076395135521 1105 68-69 -0.3666865183435952 1105 70-71 -0.5329246798860012 1105 72-73 -0.5918327092792346 1105 74-75 -0.7836966105333936 1105 76-77 -0.5722515723834682 1105 78-79 -0.8073793412084278 1105 80-81 -0.7861363570597995 1105 82-83 -0.7351995377668068 1105 84-85 -0.5824948276756174 1105 86-87 -0.41524451341530977 1105 88-89 -0.5379875308818285 1105 90-91 -0.310644566661491 1105 92-93 -0.36197242462365153 1105 94-95 -0.5633222135227456 1105 96-97 -0.9567553501017976 1105 98-99 -0.8901308076963481 1105 100-101 -0.517771198209374 1106 1 -0.4411614451455108 1106 2 -0.4037391326753905 1106 3 -0.06720902018599872 1106 4 -0.12518687698588593 1106 5 -0.25698801106667446 1106 6 -0.35020735888114984 1106 7 -0.4898207222590756 1106 8 -0.3982138024402744 1106 9 -0.3686146012720144 1106 10-11 -0.42335219865409357 1106 12-13 -0.26587149681807176 1106 14-15 -0.5388976595712549 1106 16-17 -0.7385604313001082 1106 18-19 -0.3975912241114159 1106 20-21 -0.5092049074697584 1106 22-23 -0.5189232409837246 1106 24-25 -0.4129158685353076 1106 26-27 -0.5770684856004138 1106 28-29 -0.31309987715862064 1106 30-31 -0.46443986057069253 1106 32-33 -0.5906668347681077 1106 34-35 -0.618023185415268 1106 36-37 -0.4833016778811725 1106 38-39 -0.6333011960269417 1106 40-41 -0.7342644007262749 1106 42-43 -0.3501391085048553 1106 44-45 -0.48801115039777443 1106 46-47 -0.59307666712224 1106 48-49 -0.5526474729039634 1106 50-51 -0.5703563864830059 1106 52-53 -0.5119984036543954 1106 54-55 -0.6832547681205128 1106 56-57 -0.2032474798284909 1106 58-59 -0.38366589537939433 1106 60-61 -0.20436131006579927 1106 62-63 -0.29811748158162743 1106 64-65 -0.5697396559974521 1106 66-67 -0.5430366499107748 1106 68-69 -0.3536786780014687 1106 70-71 -0.29658305366554316 1106 72-73 -0.3630994958703262 1106 74-75 -0.9028205965497555 1106 76-77 -0.897498492249067 1106 78-79 -0.2896848317106411 1106 80-81 -0.5612243204941656 1106 82-83 -1.0004842451398979 1106 84-85 -1.0458782803676954 1106 86-87 -0.755600779466512 1106 88-89 -0.6578299320855976 1106 90-91 -0.6635844121173733 1106 92-93 -0.9501225338001298 1106 94-95 -0.7437971595001862 1106 96-97 -0.4393436812289764 1106 98-99 -0.35437692429456646 1106 100-101 -0.40856077609802455 1107 1 0.27724358970382923 1107 2 0.008042012350404804 1107 3 0.20997598034498566 1107 4 0.30224433259643035 1107 5 0.29064128361845576 1107 6 0.26703677653662083 1107 7 0.10687152481073525 1107 8 0.1401987220828147 1107 9 0.22402539693940327 1107 10-11 0.007826642458624633 1107 12-13 0.38597325454151843 1107 14-15 0.41014461237097777 1107 16-17 0.3509244021105502 1107 18-19 0.486623450059831 1107 20-21 0.6078212449059777 1107 22-23 0.5191818715570093 1107 24-25 0.3993979376162855 1107 26-27 0.6305435940137016 1107 28-29 0.253974622737978 1107 30-31 0.46016595342828737 1107 32-33 0.5908962713225563 1107 34-35 0.5273435570796892 1107 36-37 0.5728373394065613 1107 38-39 0.8791378106411045 1107 40-41 1.1045287557240684 1107 42-43 0.9899507675801686 1107 44-45 1.1555753972538767 1107 46-47 1.1669910753000536 1107 48-49 1.3308881002517055 1107 50-51 1.4413140902291346 1107 52-53 1.5241700133974732 1107 54-55 1.1574806731274592 1107 56-57 1.021923413287162 1107 58-59 1.169538734803993 1107 60-61 1.2994338305891588 1107 62-63 1.4592114995432794 1107 64-65 1.392320116070536 1107 66-67 1.2461541858585647 1107 68-69 1.5130218971383975 1107 70-71 1.6661921499250312 1107 72-73 1.350951467447036 1107 74-75 1.7401092073213995 1107 76-77 1.5967584929895686 1107 78-79 1.5922494044823665 1107 80-81 1.7347351681958685 1107 82-83 1.416648663033346 1107 84-85 1.2834270244452561 1107 86-87 1.1364976147250765 1107 88-89 1.2482501179946084 1107 90-91 1.5188897513028365 1107 92-93 1.5277465981243203 1107 94-95 1.5535652456911642 1107 96-97 1.4090742649236248 1107 98-99 1.1232406887352333 1107 100-101 1.2987634541144253 1108 1 0.06958415694587572 1108 2 -0.1794975336339455 1108 3 -0.2289594695079309 1108 4 -0.03457089746407149 1108 5 -0.14721185508388146 1108 6 -0.09639220720096375 1108 7 -0.46483297649358946 1108 8 -0.03884884410467038 1108 9 0.07500502728851899 1108 10-11 0.018946695741639985 1108 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100-101 -1.068303697111439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3062.0 3 593.0 4 512.0 5 541.0 6 628.0 7 698.0 8 860.0 9 1091.0 10 1336.0 11 1833.0 12 2677.0 13 3533.0 14 3848.0 15 4117.0 16 5068.0 17 6383.0 18 5245.0 19 3465.0 20 3343.0 21 3401.0 22 3605.0 23 3984.0 24 4457.0 25 5113.0 26 5888.0 27 6665.0 28 7750.0 29 9092.0 30 10749.0 31 12633.0 32 15253.0 33 18998.0 34 24867.0 35 36674.0 36 46604.0 37 71684.0 38 82685.0 39 16879.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94016885820782 22.36153305236259 15.428648904535825 20.269649184893762 2 15.312588278255356 24.10010725449171 39.388350830131394 21.198953637121537 3 17.647410047490883 29.020649807556286 32.90465998548635 20.427280159466484 4 11.55958371901263 20.73388932401425 40.01295423068177 27.69357272629135 5 11.0561294931966 39.38896891088153 35.826343852588 13.72855774333386 6 25.178579626314097 40.20864894642611 19.748427672955977 14.864343754303816 7 22.852040418137662 36.7858306974011 24.249734876482282 16.112394007978956 8 23.336493500232997 34.98488399585888 22.80094850917405 18.877673994734074 9 24.798220483719906 18.18196426275871 24.202752832718126 32.81706242080326 10-11 21.76633232445312 28.884734001099556 29.750144330701524 19.598789343745803 12-13 23.574155397262288 28.211923722335342 29.631283300315182 18.582637580087187 14-15 21.15640878784366 29.164911523571334 29.368527252255095 20.31015243632991 16-17 18.00529567519859 31.909828906306494 30.55641317284859 19.528462245646335 18-19 17.70599470178 30.339540606678327 33.99182311107438 17.962641580467288 20-21 18.985920795579503 29.247693448452388 33.7766610729396 17.98972468302851 22-23 18.753422440186938 29.520864113770234 33.388207175156616 18.337506270886212 24-25 18.61614365119187 29.917916557111084 33.59094682430423 17.874992967392814 26-27 18.08522120540584 29.778737182946575 34.15634221676178 17.97969939488581 28-29 17.760758277297946 29.888855339731148 34.32258701509827 18.02779936787264 30-31 18.379144688886466 29.638693902851994 33.9026224107353 18.079538997526246 32-33 17.907322957832648 30.107010981165473 33.897447140156494 18.08821892084539 34-35 18.236095482024055 29.739985686441013 33.79564748143495 18.228271350099988 36-37 18.498846782922012 29.568347562683485 33.48571244148907 18.447093212905425 38-39 17.98934726041131 30.15610342975807 33.53308180391307 18.32146750591755 40-41 18.00429965805506 29.85693152806068 33.81729448740518 18.321474326479073 42-43 17.79180203364515 29.939622197917952 33.29226705451059 18.97630871392631 44-45 18.118176149703157 29.692601859994443 33.18519150289811 19.004030487404293 46-47 18.417513113051736 29.94962236946592 32.641731026558034 18.991133490924305 48-49 18.17662044241565 29.903401689894064 32.570921372664294 19.349056495025994 50-51 17.900213579408778 30.14247463960636 32.47548101471543 19.481830766269436 52-53 18.106484048371872 30.47206491079837 32.090224073201014 19.331226967628748 54-55 18.147302904564317 29.931189488243433 32.02881512217612 19.892692485016138 56-57 18.39069068515377 30.117910612877058 31.747709086215796 19.743689615753375 58-59 18.45663757596705 29.442898700999454 32.12557965379187 19.974884069241625 60-61 18.33154429819914 29.753689021159758 32.11705818126534 19.79770849937576 62-63 18.12032198028245 29.63647573335792 32.32986269165836 19.913339594701267 64-65 18.126366829715927 29.660593917413223 32.11440271054509 20.09863654232575 66-67 18.171557692196842 29.670684397715025 31.41300776453906 20.74475014554908 68-69 18.231794493381912 29.240764654337497 31.833118110962506 20.694322741318082 70-71 18.503918851300405 29.307104661807397 31.82086502964215 20.36811145725005 72-73 18.532523422882313 28.75166729880877 31.983085491358864 20.73272378695005 74-75 18.267938369886732 29.47557227797643 31.452291532660592 20.80419781947624 76-77 18.516563435734422 28.978924292962237 31.619899635099593 20.884612636203745 78-79 18.512530967333067 28.82053350233335 31.728178832747595 20.93875669758599 80-81 18.730662106428852 28.766603940349693 31.378177464389466 21.12455648883199 82-83 18.81399101071799 28.713034458914372 31.467788406131152 21.005186124236488 84-85 18.623776662195116 28.459689204202338 31.38993762878756 21.526596504814986 86-87 18.768952731556134 28.429414293601223 31.406527519567923 21.395105455274724 88-89 19.235433256025196 27.905856206859987 31.47647788418534 21.382232652929485 90-91 19.28017217033059 27.60419728580436 31.65370432489196 21.461926218973087 92-93 19.698206298704292 27.765827454235254 31.391271268501868 21.144694978558583 94-95 19.678509301304153 27.1041052084306 31.422234364967107 21.79515112529814 96-97 19.811007537103347 26.861968869388384 31.330279142582445 21.996744450925824 98-99 19.99259930419626 26.77760793834137 31.079464106625355 22.150328650837018 100-101 20.310376663658335 26.240359800873915 31.647285414440674 21.801978121027073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3958.0 1 2406.5 2 985.0 3 1087.5 4 860.5 5 794.5 6 1266.0 7 2017.5 8 2750.0 9 2917.5 10 2221.5 11 1568.0 12 1409.0 13 1353.0 14 1356.5 15 1260.0 16 1186.0 17 1265.0 18 1323.0 19 1377.5 20 1502.5 21 1670.5 22 1895.0 23 2213.5 24 2590.5 25 3184.5 26 4165.0 27 5224.0 28 5908.5 29 6684.5 30 7838.0 31 8977.0 32 10515.5 33 12456.0 34 14523.0 35 15960.0 36 17172.5 37 18638.0 38 18501.0 39 17494.5 40 16749.5 41 16042.0 42 15371.0 43 15010.0 44 14890.5 45 14351.5 46 13679.5 47 13575.0 48 14803.5 49 14713.5 50 12452.0 51 10271.5 52 8543.0 53 7411.0 54 6467.0 55 5531.5 56 4761.5 57 4028.5 58 3495.0 59 3067.5 60 2531.5 61 2051.0 62 1650.5 63 1290.5 64 997.5 65 787.0 66 607.0 67 480.5 68 366.0 69 279.5 70 217.5 71 165.5 72 136.0 73 120.5 74 102.0 75 86.0 76 73.0 77 46.5 78 31.5 79 27.0 80 19.0 81 10.0 82 7.0 83 6.5 84 4.5 85 5.5 86 5.0 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15258487964150586 2 0.09407488820002616 3 0.0851263012736822 4 0.10187622039222344 5 0.12069119803222869 6 0.03740050433318113 7 0.04061281758879178 8 0.04405458179123176 9 0.04566073841903709 10-11 0.0439398563178171 12-13 0.04680799315318374 14-15 0.04554601294562242 16-17 0.04233369969001177 18-19 0.04703744410001308 20-21 0.04956140451513573 22-23 0.06390208869196898 24-25 0.08065200781051023 26-27 0.08443794843319422 28-29 0.07101506804367828 30-31 0.057477462180747695 32-33 0.048987777148062406 34-35 0.2914027024732518 36-37 0.46727685321793483 38-39 0.4460526406362216 40-41 0.4210424874318244 42-43 0.4523625416740282 44-45 0.4599344229193962 46-47 0.4587871681852495 48-49 0.4745045580430588 50-51 0.4649823437496415 52-53 0.4723247740481801 54-55 0.46417926543573884 56-57 0.44398758211475764 58-59 0.42115721290523905 60-61 0.38937825676937654 62-63 0.44639681705646556 64-65 0.484026772336476 66-67 0.4865507327515987 68-69 0.49630239799184533 70-71 0.493319535683064 72-73 0.4847151251769641 74-75 0.4758812637240348 76-77 0.46027859933964016 78-79 0.4354978970820723 80-81 0.44226670001353763 82-83 0.4527067180942722 84-85 0.4517889143069549 86-87 0.46199948144086017 88-89 0.47289840141525347 90-91 0.4741603816228148 92-93 0.47794632224549877 94-95 0.48035555718720674 96-97 0.48230589023525605 98-99 0.4772579694050107 100-101 0.4652117946964708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 435823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.79114367747428 #Duplication Level Percentage of deduplicated Percentage of total 1 87.71195517689752 52.443981142140636 2 6.9512547064631445 8.312469377857145 3 1.771190036260189 3.177044338144315 4 0.7841557917189468 1.8754228643276458 5 0.49978802009914547 1.4941448659014207 6 0.32983711965377605 1.183280316682992 7 0.23602796358329223 0.9878667317757205 8 0.2052335446052289 0.9816918682342854 9 0.1597923769192103 0.8598752070247467 >10 1.18662967220714 13.945054565923689 >50 0.10993625104514115 4.58929862854973 >100 0.049971023199645984 4.988747797838804 >500 0.0015375699446044918 0.6917963176769003 >1k 0.0026907474030578604 4.469325977921928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4537 1.0410189457646797 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4267 0.97906719012076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.856769835460726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2440 0.5598603102635703 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.537603568421125 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1083 0.24849537541616665 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1042 0.23908788659616403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.20191683320981224 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 766 0.1757594252712684 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 700 0.16061566278053246 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 664 0.1523554286946765 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 483 0.11082480731856739 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.441764202439981E-4 0.0 20-21 0.0 0.0 0.0 0.0011472547341466605 0.0 22-23 0.0 0.0 0.0 0.004589018936586642 0.0 24-25 0.0 0.0 0.0 0.010669469027563942 0.0 26-27 0.0 0.0 0.0 0.019732781427322557 0.0 28-29 0.0 0.0 0.0 0.05334734513781971 0.0 30-31 0.0 0.0 0.0 0.12700109907003532 0.0 32-33 0.0 0.0 0.0 0.2505604339376306 0.0 34-35 0.0 0.0 0.0 0.4629172852281775 0.0 36-37 0.0 0.0 0.0 0.7883934533055851 0.0 38-39 2.2945094682933208E-4 0.0 0.0 1.1675611429410564 0.0 40-41 2.2945094682933208E-4 0.0 0.0 1.5703622800999488 0.0 42-43 2.2945094682933208E-4 0.0 0.0 1.9549220669859095 0.0 44-45 2.2945094682933208E-4 0.0 0.0 2.3853720432377363 0.0 46-47 2.2945094682933208E-4 0.0 0.0 2.877544324186654 0.0 48-49 2.2945094682933208E-4 0.0 0.0 3.3813038779504527 0.0 50-51 2.2945094682933208E-4 0.0 0.0 3.9076643499769403 0.0 52-53 2.2945094682933208E-4 0.0 0.0 4.392838377047563 0.0 54-55 2.2945094682933208E-4 0.0 0.0 4.92459094632454 0.0 56-57 2.2945094682933208E-4 0.0 0.0 5.580935379729844 0.0 58-59 2.2945094682933208E-4 0.0 0.0 6.189783467141477 0.0 60-61 2.2945094682933208E-4 0.0 0.0 6.85197889969093 0.0 62-63 2.2945094682933208E-4 0.0 0.0 7.500866177324281 0.0 64-65 2.2945094682933208E-4 0.0 0.0 8.188645390445203 0.0 66-67 2.2945094682933208E-4 0.0 0.0 8.953405396227367 0.0 68-69 2.2945094682933208E-4 0.0 0.0 9.78229694164833 0.0 70-71 3.441764202439981E-4 0.0 0.0 10.676467281442237 0.0 72-73 4.5890189365866416E-4 0.0 0.0 11.514192688316129 0.0 74-75 4.5890189365866416E-4 0.0 0.0 12.380828914490515 0.0 76-77 4.5890189365866416E-4 0.0 0.0 13.308957994415163 0.0 78-79 4.5890189365866416E-4 0.0 0.0 14.332768119167643 0.0 80-81 4.5890189365866416E-4 0.0 0.0 15.401780080445501 0.0 82-83 4.5890189365866416E-4 0.0 0.0 16.429835047714327 0.0 84-85 4.5890189365866416E-4 0.0 0.0 17.476360816202906 0.0 86-87 4.5890189365866416E-4 0.0 0.0 18.536309465081008 0.0 88-89 4.5890189365866416E-4 0.0 0.0 19.649834910043758 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCCGG 25 0.0050676437 56.861782 1 GCGAGAT 50 0.001647052 37.84692 7 TTAGCGA 55 0.00262497 34.418144 4 TAGCGAG 55 0.00262497 34.418144 5 ATTAGCG 65 0.005946449 29.123047 3 GGATATG 170 3.7234713E-9 27.873423 1 CGCAGAA 115 1.3145019E-4 24.685608 9 GGTATCA 4025 0.0 24.369335 1 ATACTCA 100 0.001692403 23.651608 6 ATGGGAT 535 0.0 22.99904 5 ACCCATG 215 4.5813067E-8 22.011604 3 CATATAG 130 3.0116958E-4 21.842285 3 GAGTACT 2875 0.0 21.725828 12-13 GTACATG 2630 0.0 21.620451 1 CATTCCG 110 0.0029406864 21.5064 9 CCGACTA 110 0.0029406864 21.5064 9 CTAGACC 135 3.8864068E-4 21.033312 4 GGCCGAC 135 3.8954604E-4 21.026066 7 TACATGG 2680 0.0 20.837105 2 TACCCAT 230 9.345422E-8 20.576065 2 >>END_MODULE