FastQCFastQC Report
Thu 26 May 2016
SRR938807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435823
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45231.0378066325090691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41460.951303625554411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.8923347322192725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24670.5660554858279623No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.49194283000208794No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16750.38433033593913124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12260.28130686081276113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.2393173375429934No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT7990.18333130651663634TruSeq Adapter, Index 1 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.1753005233776097No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7450.1709409553878524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.16268072130199646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1422595870341859No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4600.10554743554149276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG1353.110472E-1035.142613
CGCAGAA1301.0096859E-525.545979
GGATATG1901.2021701E-824.9697469
GGTATCA43400.024.0558051
CATGGGT2853.6379788E-1223.3050984
GACATAT2154.2398824E-822.1731341
GAGTACT31250.021.71218912-13
GTATCAA54900.020.9271581
CATGGGG6350.020.9195354
GATATAG1604.8431768E-520.8566041
TATAGTA2351.14232535E-720.1883052
GTCCTAA1907.9782985E-620.0725211
GTACTTT33600.020.05241414-15
GTACATG26450.019.8258861
ACCATAC1200.004792289319.7677173
GTCCAAG1455.989777E-419.7264421
AGTACTT32150.019.5547612-13
TACATGG27000.019.5041472
GTATAGT1759.6468924E-519.0688951
ACATGGG26200.019.0132243
Produced by FastQC (version 0.11.5)