##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938807_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 435823 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900627089437684 31.0 31.0 34.0 30.0 34.0 2 32.1152876282344 33.0 31.0 34.0 30.0 34.0 3 32.16704258380122 34.0 31.0 34.0 30.0 34.0 4 35.85055859833005 37.0 35.0 37.0 35.0 37.0 5 35.76886488322094 37.0 35.0 37.0 33.0 37.0 6 35.80498275676135 37.0 35.0 37.0 33.0 37.0 7 35.74122292765641 37.0 35.0 37.0 33.0 37.0 8 35.72992476303453 37.0 35.0 37.0 33.0 37.0 9 37.336765154661414 39.0 37.0 39.0 34.0 39.0 10-11 37.2842816923384 39.0 37.0 39.0 33.0 39.0 12-13 37.229548463481734 39.0 37.0 39.0 33.0 39.0 14-15 38.39081576695126 40.0 38.0 41.0 33.0 41.0 16-17 38.31724690986937 40.0 38.0 41.0 33.0 41.0 18-19 38.35624898181142 40.0 37.5 41.0 33.5 41.0 20-21 38.413920330042245 40.0 38.0 41.0 34.0 41.0 22-23 38.33124227037123 40.0 38.0 41.0 33.5 41.0 24-25 38.23543846928685 40.0 37.0 41.0 33.0 41.0 26-27 38.028060703542494 40.0 37.0 41.0 33.0 41.0 28-29 37.84324370214513 40.0 37.0 41.0 33.0 41.0 30-31 37.53587695004623 39.5 36.0 41.0 32.5 41.0 32-33 37.19974737450754 39.0 35.5 41.0 32.0 41.0 34-35 37.03677754501254 39.0 35.0 41.0 31.0 41.0 36-37 36.87963347505753 39.0 35.0 41.0 31.0 41.0 38-39 36.7020407367211 39.0 35.0 41.0 31.0 41.0 40-41 36.51735452236343 39.0 35.0 41.0 30.0 41.0 42-43 36.27246037955776 39.0 35.0 41.0 30.0 41.0 44-45 35.93776946145569 39.0 35.0 41.0 29.5 41.0 46-47 35.50613551831822 39.0 35.0 41.0 26.5 41.0 48-49 35.13176908974515 39.0 35.0 41.0 24.5 41.0 50-51 34.865857240209905 39.0 35.0 41.0 24.0 41.0 52-53 34.692601124768544 39.0 35.0 40.5 23.5 41.0 54-55 34.45394575320715 38.0 34.0 40.0 23.0 41.0 56-57 34.174337976655664 38.0 34.0 40.0 21.5 41.0 58-59 33.87840247072779 38.0 34.0 40.0 20.0 41.0 60-61 33.521017706729566 38.0 33.0 40.0 17.5 41.0 62-63 33.14489024213958 37.0 33.0 40.0 13.5 41.0 64-65 32.77120298836913 37.0 33.0 40.0 8.0 41.0 66-67 32.3213231059398 36.0 32.0 39.0 4.5 41.0 68-69 31.854643972438353 36.0 31.5 39.0 2.0 40.0 70-71 31.375505652524076 35.0 31.0 38.5 2.0 40.0 72-73 31.038352725762522 35.0 31.0 38.0 2.0 40.0 74-75 30.61221413280162 35.0 31.0 37.0 2.0 39.5 76-77 28.546070308359127 33.0 27.5 35.5 2.0 37.5 78-79 29.565229462419374 34.0 29.5 36.0 2.0 39.0 80-81 29.55548123894333 34.0 30.0 36.0 2.0 38.0 82-83 29.213720937169448 34.0 29.5 35.5 2.0 37.0 84-85 28.796782179921664 34.0 29.0 35.0 2.0 37.0 86-87 28.278945810569887 34.0 29.0 35.0 2.0 36.0 88-89 27.75868873372906 34.0 27.5 35.0 2.0 36.0 90-91 27.340639433898623 34.0 26.5 35.0 2.0 35.5 92-93 27.054469360267817 33.5 26.0 35.0 2.0 35.0 94-95 26.687245739669546 33.0 25.5 35.0 2.0 35.0 96-97 26.21236373481895 33.0 25.0 35.0 2.0 35.0 98-99 25.55772182743912 33.0 22.0 35.0 2.0 35.0 100-101 22.791087207421363 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.011103853790036311 1101 2 0.0861723184990808 1101 3 0.042060966484136486 1101 4 0.10673236226428173 1101 5 0.06323160730438815 1101 6 0.28346873680742846 1101 7 0.20353147739346866 1101 8 0.3158419468521032 1101 9 0.22007718466421267 1101 10-11 0.20403306798544207 1101 12-13 0.1950055344108037 1101 14-15 0.3514228098397538 1101 16-17 0.4508493655153032 1101 18-19 0.35339075524829155 1101 20-21 0.2141218892946739 1101 22-23 0.16362258744634772 1101 24-25 0.15927889232597892 1101 26-27 0.30727243687434225 1101 28-29 0.22456232708218238 1101 30-31 0.1379316442832561 1101 32-33 0.025370870071036222 1101 34-35 -0.20437492052968054 1101 36-37 -0.055243793160407506 1101 38-39 -0.1766793175514465 1101 40-41 -0.2974033921430177 1101 42-43 -0.2543125553110812 1101 44-45 -0.37252351354847235 1101 46-47 -0.3112720512712528 1101 48-49 -0.5059715719358664 1101 50-51 -0.40170923313928597 1101 52-53 -0.49825922948569 1101 54-55 -0.6832392837604928 1101 56-57 -0.480198189114752 1101 58-59 -0.5940002666063364 1101 60-61 -0.35088986339592054 1101 62-63 -0.5572444294779828 1101 64-65 -0.5726887533554077 1101 66-67 -0.49050369770653646 1101 68-69 -0.3459667788421008 1101 70-71 -0.4886849743258317 1101 72-73 -0.4817035371453571 1101 74-75 -0.2257516426129378 1101 76-77 -0.12632404652502416 1101 78-79 -0.3974387872462408 1101 80-81 -0.13809896772191266 1101 82-83 -0.33757925285176427 1101 84-85 -0.25270873938803007 1101 86-87 -0.07896012139732989 1101 88-89 0.06717648678342059 1101 90-91 0.006512327678880325 1101 92-93 0.273699046958118 1101 94-95 -0.2034161061985742 1101 96-97 -0.5704087768564818 1101 98-99 -0.331901895593699 1101 100-101 0.5590396576929137 1102 1 0.25884821469228925 1102 2 0.11564600270961023 1102 3 0.1890095880380187 1102 4 0.14680755023420744 1102 5 0.09613887547481426 1102 6 0.21073690723349614 1102 7 0.19863799368419421 1102 8 0.01567903958393657 1102 9 0.24866741022812278 1102 10-11 0.07906752913832094 1102 12-13 0.06265904318273385 1102 14-15 0.17329624342371375 1102 16-17 0.1976444782972564 1102 18-19 0.19278611865680517 1102 20-21 0.3362741449337747 1102 22-23 0.27234125912554674 1102 24-25 0.2257387920753544 1102 26-27 0.34516717371644745 1102 28-29 0.37185556016489585 1102 30-31 0.5437023209749796 1102 32-33 0.2685569602965998 1102 34-35 0.32224162082370356 1102 36-37 0.5301666078420979 1102 38-39 0.43449862731070965 1102 40-41 0.7870483622429489 1102 42-43 0.8881153895510749 1102 44-45 0.8673135791833602 1102 46-47 0.7032830865733644 1102 48-49 0.5849432150315508 1102 50-51 0.37836908766271904 1102 52-53 0.5737865098626784 1102 54-55 0.2823496886705854 1102 56-57 0.2080129637674517 1102 58-59 0.4377541193585728 1102 60-61 0.4787060012657278 1102 62-63 0.845048803604719 1102 64-65 0.44431939200799775 1102 66-67 0.6061786331205283 1102 68-69 0.3303176823107812 1102 70-71 0.3341220432180272 1102 72-73 0.4832588688696795 1102 74-75 0.8946148987404428 1102 76-77 0.9001013920714698 1102 78-79 0.8084196087437334 1102 80-81 0.7621641901728289 1102 82-83 0.9040028273487373 1102 84-85 1.0821596816646029 1102 86-87 0.8954947658207146 1102 88-89 1.2350524266330432 1102 90-91 1.256888267528506 1102 92-93 1.1553343853039841 1102 94-95 1.1477430416711023 1102 96-97 1.154093102842765 1102 98-99 1.4887090066619386 1102 100-101 1.6454838932818845 1103 1 0.08195334916661778 1103 2 0.14703964574139405 1103 3 0.034223524468337985 1103 4 -0.07346139842104549 1103 5 0.06232469454572254 1103 6 -0.05376187027261636 1103 7 0.10677369050571173 1103 8 0.12598466305581724 1103 9 0.2576344028931601 1103 10-11 0.21904002302585468 1103 12-13 0.12315720944190645 1103 14-15 0.09590626787359469 1103 16-17 -0.020592074269977445 1103 18-19 -0.08039542168548763 1103 20-21 0.13614883931030164 1103 22-23 0.1889885696389868 1103 24-25 -0.030599838731497186 1103 26-27 0.325802870537963 1103 28-29 0.5000462692113459 1103 30-31 0.2909455972586059 1103 32-33 0.2924719969714218 1103 34-35 0.2160802516305509 1103 36-37 0.2433909354704653 1103 38-39 0.3605132972373539 1103 40-41 0.48207586835541605 1103 42-43 0.6288458296488741 1103 44-45 0.41805930045475037 1103 46-47 0.12325252422616728 1103 48-49 0.30126350843008964 1103 50-51 0.5367095522103966 1103 52-53 0.6048775856572988 1103 54-55 0.8115183928270611 1103 56-57 0.914287413645205 1103 58-59 0.46105485285490033 1103 60-61 0.8603166369625583 1103 62-63 0.9065035713308802 1103 64-65 1.1755571670691225 1103 66-67 0.9012550389885021 1103 68-69 0.9453237947802187 1103 70-71 0.7930462485970011 1103 72-73 0.3846769617792134 1103 74-75 0.2717206444617162 1103 76-77 0.8081545705555762 1103 78-79 0.48626190703224026 1103 80-81 0.27278766205546745 1103 82-83 0.2402406024098589 1103 84-85 0.3853687281193672 1103 86-87 0.5562954993170486 1103 88-89 0.46571868580175035 1103 90-91 0.8573772650835174 1103 92-93 0.6955605466731782 1103 94-95 0.7211232372701275 1103 96-97 0.3855998265591474 1103 98-99 0.5395983709651127 1103 100-101 0.7907681231107375 1104 1 0.07304869201925968 1104 2 0.1238560265759574 1104 3 0.15086519662990128 1104 4 0.1582872638380266 1104 5 0.08055414993782506 1104 6 0.042765546851633474 1104 7 0.07907355454338472 1104 8 0.007242285407322413 1104 9 0.2623726608247807 1104 10-11 0.20230440264667493 1104 12-13 0.24686250380326413 1104 14-15 0.22012798566715475 1104 16-17 0.19872741385811565 1104 18-19 0.37287263416993 1104 20-21 0.2182222356258947 1104 22-23 -0.014779862592831705 1104 24-25 0.37569702596557164 1104 26-27 0.005739966079211456 1104 28-29 0.1728818131481873 1104 30-31 0.3459636574904934 1104 32-33 0.18644180516533737 1104 34-35 0.20954472344899955 1104 36-37 0.34877340975140925 1104 38-39 0.7443792955684714 1104 40-41 0.4856849970878159 1104 42-43 0.36125679766563223 1104 44-45 0.6673255123575856 1104 46-47 0.6122150674802853 1104 48-49 0.889776150592418 1104 50-51 0.9840343621257261 1104 52-53 0.7651439084307015 1104 54-55 0.7731134118209368 1104 56-57 0.8257426773712666 1104 58-59 0.981262472580525 1104 60-61 1.1175276003110781 1104 62-63 1.2144640780677278 1104 64-65 1.2663689146810313 1104 66-67 0.8482997548389051 1104 68-69 0.9768868947212859 1104 70-71 0.8930719238862821 1104 72-73 0.9471729500152648 1104 74-75 0.9351847078096576 1104 76-77 0.8424194111645456 1104 78-79 0.9101200860707017 1104 80-81 0.9146582235857146 1104 82-83 0.9396442354632946 1104 84-85 1.1087408272493242 1104 86-87 1.2006081309758478 1104 88-89 1.2903447894015407 1104 90-91 1.1342152365022535 1104 92-93 1.082965650220455 1104 94-95 1.0394793185207476 1104 96-97 0.965426635062336 1104 98-99 1.2740341856595485 1104 100-101 1.26083890521506 1105 1 0.06053903594833088 1105 2 0.027819915753084956 1105 3 -0.14708944577840555 1105 4 -0.022612739620861078 1105 5 -0.056469820177355246 1105 6 -0.1377655082254421 1105 7 0.016669394650378422 1105 8 0.20236986083997266 1105 9 0.14892103341652785 1105 10-11 -0.09256894429162799 1105 12-13 0.08974237651607098 1105 14-15 0.15393634004207257 1105 16-17 -0.019511318804198652 1105 18-19 0.13689843749738628 1105 20-21 0.06536230918497665 1105 22-23 -0.11219376502904055 1105 24-25 -0.09613318860098019 1105 26-27 -0.19787630454442962 1105 28-29 0.006589859681803034 1105 30-31 0.06697526783488428 1105 32-33 -0.194154392360403 1105 34-35 -0.28360378980431733 1105 36-37 -0.2763490926409915 1105 38-39 -0.23569460940426978 1105 40-41 -0.39834414983434385 1105 42-43 -0.25173364426535727 1105 44-45 -0.44734825656543364 1105 46-47 -0.44332319361986805 1105 48-49 -0.3369166883500796 1105 50-51 -0.2834243905981566 1105 52-53 -0.37561566405036473 1105 54-55 -0.10223968331009559 1105 56-57 -0.4831766497591232 1105 58-59 -0.2412796970665454 1105 60-61 -0.41351018230914605 1105 62-63 -0.6049270418059081 1105 64-65 -0.2525223954316118 1105 66-67 -0.17143610117899044 1105 68-69 -0.17155429836554603 1105 70-71 0.08098194659967106 1105 72-73 -0.4122000683283886 1105 74-75 -0.1189901737233221 1105 76-77 -0.007839429184571145 1105 78-79 -0.026302613478488723 1105 80-81 0.028758393071377952 1105 82-83 -0.09846697941454963 1105 84-85 -0.07466398983298461 1105 86-87 -0.07236755301986264 1105 88-89 0.27552344112579874 1105 90-91 0.21969019989565552 1105 92-93 0.16213390221219726 1105 94-95 0.49695801751651203 1105 96-97 -0.10540568942776574 1105 98-99 -0.47039123488395873 1105 100-101 -0.022686396573185164 1106 1 -0.21433679701731023 1106 2 -0.23910809565104785 1106 3 -0.14804544709078726 1106 4 -0.17466785960185405 1106 5 -0.1626199067265901 1106 6 -0.13479002485081537 1106 7 -0.4634638798521067 1106 8 -0.22004695573222222 1106 9 -0.1892658450411986 1106 10-11 -0.2833534310490293 1106 12-13 -0.4157221043582453 1106 14-15 -0.37461359264185745 1106 16-17 -0.6179907676043896 1106 18-19 -0.22452945511368227 1106 20-21 -0.3875051290709095 1106 22-23 -0.3301733778767968 1106 24-25 -0.7075808800557937 1106 26-27 -0.5676197115294528 1106 28-29 -0.4225472501395515 1106 30-31 -0.46597566497349163 1106 32-33 -0.3149471380640563 1106 34-35 -0.5226295735556903 1106 36-37 -0.2072426427433811 1106 38-39 -0.08723439376657183 1106 40-41 -0.23684215297953415 1106 42-43 -0.5114718469828858 1106 44-45 -0.46930234587518527 1106 46-47 -0.6660319017170409 1106 48-49 -0.8203846538209021 1106 50-51 -0.6537532776768558 1106 52-53 -0.5371063472801794 1106 54-55 -0.5764559319383196 1106 56-57 -0.764823687286416 1106 58-59 -0.45899061132057994 1106 60-61 -0.5137149589216179 1106 62-63 -0.7769535383156594 1106 64-65 -0.47786152134094806 1106 66-67 -0.6114823739052326 1106 68-69 -0.7502151045744689 1106 70-71 -0.938477488397897 1106 72-73 -0.8468582271490561 1106 74-75 -0.5522030169504255 1106 76-77 -0.666218865539772 1106 78-79 -0.39600076596352807 1106 80-81 -0.47780068102155226 1106 82-83 -0.5326160251102792 1106 84-85 -0.6999539014735738 1106 86-87 -0.3215953981137112 1106 88-89 -0.43584335791028295 1106 90-91 -0.518755760574539 1106 92-93 -0.510104724766272 1106 94-95 -0.20111527214622527 1106 96-97 -0.47808781050618165 1106 98-99 -0.47747073572682197 1106 100-101 0.1671788581530791 1107 1 -0.0343498759998333 1107 2 -0.14583371089420893 1107 3 -0.16655723773763498 1107 4 -0.08753612518345477 1107 5 -0.10321673311706547 1107 6 0.009352659023470267 1107 7 -0.04980472707953254 1107 8 0.06929479137391326 1107 9 -0.03118342509407057 1107 10-11 0.04001323319560157 1107 12-13 -0.2113713864115354 1107 14-15 -0.2691321575309402 1107 16-17 0.034049609562167404 1107 18-19 0.19029506853747336 1107 20-21 0.15855636450417876 1107 22-23 -0.02790635423961163 1107 24-25 0.055887956753160495 1107 26-27 -0.030059556593819536 1107 28-29 0.1895882570622618 1107 30-31 0.024961270855648365 1107 32-33 0.05279025385268454 1107 34-35 0.3563227664084252 1107 36-37 0.3070072999662017 1107 38-39 0.2575057872152442 1107 40-41 0.2780477655842333 1107 42-43 0.17509927976587392 1107 44-45 0.1374925767967241 1107 46-47 0.08118881449698989 1107 48-49 0.15946588806257722 1107 50-51 0.34441622370090386 1107 52-53 0.2902035743018203 1107 54-55 0.4723974213674822 1107 56-57 0.36989542200134196 1107 58-59 0.1411670071867377 1107 60-61 0.0077423974471244605 1107 62-63 0.08797243128968546 1107 64-65 0.2150562655163526 1107 66-67 0.1370825710680279 1107 68-69 0.37784154865923014 1107 70-71 0.03149674488867049 1107 72-73 0.003258868869679077 1107 74-75 -0.14238092464857743 1107 76-77 0.09540270472063384 1107 78-79 0.24066901208502145 1107 80-81 0.21895416630647802 1107 82-83 0.5637492473964691 1107 84-85 0.2853518057696114 1107 86-87 0.6767417825271593 1107 88-89 0.810631185582924 1107 90-91 0.9981770503447329 1107 92-93 0.9719871299340568 1107 94-95 1.380768101336976 1107 96-97 0.9630400002174682 1107 98-99 0.6737955221750624 1107 100-101 1.152247019773533 1108 1 -0.17952387833096495 1108 2 0.019832049221236048 1108 3 -0.05988576079919028 1108 4 -0.07249477534718807 1108 5 -0.013396008246111535 1108 6 -0.17437937183627383 1108 7 -0.11432712259487943 1108 8 -0.19350700692768896 1108 9 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-0.2900501230800465 2316 78-79 -0.16676978519566177 2316 80-81 -0.1673055067968683 2316 82-83 -0.5399744453785367 2316 84-85 -0.5619691062141889 2316 86-87 -0.7411340220580733 2316 88-89 -0.9027127248821074 2316 90-91 -1.0552329445927064 2316 92-93 -1.402658038938437 2316 94-95 -1.833506958328897 2316 96-97 -1.4240129577632956 2316 98-99 -1.8848894781865475 2316 100-101 -2.768569137021146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 26.0 6 94.0 7 214.0 8 333.0 9 546.0 10 985.0 11 1392.0 12 1895.0 13 2632.0 14 2997.0 15 3999.0 16 4773.0 17 6331.0 18 5967.0 19 3856.0 20 3322.0 21 3423.0 22 3668.0 23 3864.0 24 4462.0 25 5019.0 26 5535.0 27 6261.0 28 7596.0 29 9037.0 30 10827.0 31 13068.0 32 15560.0 33 19458.0 34 25552.0 35 37048.0 36 48226.0 37 80004.0 38 87056.0 39 10784.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18961537929986 23.51903294313842 15.644420489148711 20.646931188413006 2 15.370689477150126 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18.982935733084304 29.506588683938205 33.2488189012512 18.261656681726297 22-23 18.647363723346405 30.11474841850935 32.947779259011114 18.290108599133134 24-25 18.630843255174693 30.50561810643312 33.184802087085814 17.67873655130638 26-27 18.30483546034849 30.28254654423136 33.735102863095484 17.677515132324665 28-29 17.715104526378827 30.319992290448187 33.996943713388234 17.967959469784752 30-31 18.484338825624164 30.056926779908355 33.46312608558979 17.995608308877685 32-33 17.982070703014756 30.332726817997212 33.54882601423055 18.136376464757483 34-35 18.083478093253692 30.231034538148926 33.70745263080598 17.978034737791404 36-37 18.5595981085029 29.944267159621745 33.11669013252184 18.379444599353512 38-39 17.90637876018464 30.4305172609643 33.181646592842725 18.481457386008334 40-41 17.85539724682012 30.14832934438154 33.63305926978088 18.363214139017455 42-43 17.818810541907077 30.17827988990451 33.23435906320165 18.768550504986766 44-45 18.040849761310092 30.12598192129027 32.91660595686488 18.916562360534765 46-47 18.5887228154872 30.165114795625698 32.21333217076853 19.032830218118573 48-49 18.211980551737746 30.024344745458592 32.262868182725555 19.500806520078104 50-51 17.74840272176899 30.24738452501552 32.32989804265298 19.674314710562516 52-53 18.03702420478038 30.656367378499986 32.08068413094306 19.225924285776568 54-55 17.898665283842295 30.23348928349353 31.68270146366759 20.185143968996588 56-57 18.32808273083339 30.381829320618692 31.194200397867945 20.09588755067998 58-59 18.12563385969501 30.185603296758767 31.6528307428262 20.035932100720018 60-61 18.196962048552155 30.870428158414025 31.074640906796386 19.857968886237437 62-63 17.710056605548584 30.78371265399027 31.34070482741847 20.165525913042682 64-65 17.69158580432882 30.7636356961427 31.175385420227936 20.36939307930054 66-67 17.91342038027298 30.63385331278961 30.45006120667204 21.002665100265364 68-69 17.886836438396085 30.127541040405397 30.851807108660562 21.13381541253796 70-71 18.20140542090922 30.102165495482573 30.7913380180697 20.905091065538507 72-73 18.23962939083068 29.569572968842856 30.846123311527844 21.344674328798618 74-75 17.798854121971534 30.21846024647621 30.28913113809964 21.693554493452616 76-77 17.933647307960417 29.769020787325257 30.70658071984483 21.5907511848695 78-79 17.88644105144284 29.614408219102618 30.653272675523564 21.845878053930974 80-81 18.068458984496 29.479054570318684 30.24599436009573 22.20649208508959 82-83 17.977940585971826 29.32233957363425 30.58328725193484 22.116432588459077 84-85 17.809867767419345 28.97644227128903 30.471429915355547 22.74226004593608 86-87 17.81260125888493 29.05845610793594 30.400301507306725 22.728641125872407 88-89 18.027044883733243 28.61996965853187 30.297728533641504 23.05525692409338 90-91 17.876602637852166 28.317563898425202 30.587948347430714 23.21788511629191 92-93 18.161042441541635 28.482893284659138 30.401791552992847 22.954272720806383 94-95 17.86897188649049 27.897137022526376 30.626114989473923 23.607776101509216 96-97 18.02222461870989 27.743831784921863 30.503094146017993 23.73084945035026 98-99 17.949603394038405 27.736145178203074 30.2715781406672 24.04267328709132 100-101 17.946461657777263 27.383188184989855 30.86388026208158 23.80646989515131 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3664.0 1 2271.5 2 973.5 3 1020.0 4 815.5 5 765.5 6 1167.0 7 1940.5 8 2570.0 9 2662.5 10 2079.0 11 1426.5 12 1251.0 13 1166.0 14 1127.0 15 1131.0 16 1119.0 17 1102.0 18 1134.0 19 1277.5 20 1432.0 21 1584.0 22 1819.0 23 2107.0 24 2436.5 25 2964.0 26 3987.0 27 5153.0 28 5877.0 29 6570.0 30 7704.5 31 8876.5 32 10589.5 33 12696.5 34 14689.0 35 16121.5 36 17321.5 37 18660.0 38 18558.5 39 17551.5 40 16879.5 41 16202.0 42 15525.5 43 15381.0 44 15310.5 45 15020.0 46 14340.0 47 14064.5 48 15267.5 49 15060.5 50 13226.0 51 11280.0 52 8870.0 53 7047.0 54 6098.0 55 5369.0 56 4639.5 57 3975.0 58 3431.0 59 2938.0 60 2382.0 61 1896.5 62 1533.0 63 1174.0 64 817.0 65 614.5 66 470.0 67 364.5 68 273.0 69 193.0 70 141.5 71 121.5 72 110.5 73 85.5 74 63.5 75 45.0 76 30.5 77 18.0 78 9.5 79 7.5 80 6.0 81 4.0 82 2.5 83 1.5 84 1.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38570704162010727 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.294509468293321E-4 16-17 0.002409234941707987 18-19 2.294509468293321E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.294509468293321E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010095841660490611 36-37 0.019159154060249227 38-39 0.012963978495857264 40-41 0.008260234085855954 42-43 0.0044742934631719754 44-45 0.0014914311543906588 46-47 0.0011472547341466605 48-49 0.0 50-51 8.030783139026623E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.294509468293321E-4 60-61 6.883528404879963E-4 62-63 0.0 64-65 0.0 66-67 0.0010325292607319943 68-69 5.736273670733302E-4 70-71 0.002409234941707987 72-73 0.0 74-75 0.0 76-77 0.10038478923783278 78-79 0.035564896758546474 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1551088400566285 88-89 0.3290326577532622 90-91 0.2815363117595905 92-93 0.0 94-95 1.1472547341466605E-4 96-97 0.0 98-99 0.0 100-101 0.0010325292607319943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 435823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.961846618993185 #Duplication Level Percentage of deduplicated Percentage of total 1 86.85651977070691 50.34364276809262 2 7.487657567862917 8.679969189680278 3 1.8947637449911268 3.294720166992144 4 0.8222954339858612 1.9064704728074773 5 0.512428526726619 1.4850651834662472 6 0.3644380381106195 1.2674101000256712 7 0.2573076050396013 1.0439816756044096 8 0.20530453515751496 0.951986398158686 9 0.16536409240863306 0.8626327344430385 >10 1.2525833448960544 14.239573003066017 >50 0.12036364933451071 4.785557289338451 >100 0.055826561038532105 5.459110765666367 >500 0.0019796652850543298 0.8346555345017899 >1k 0.0031674644560869274 4.8452247181568024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4523 1.0378066325090691 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4146 0.951303625554411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.8923347322192725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2467 0.5660554858279623 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2144 0.49194283000208794 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1675 0.38433033593913124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1226 0.28130686081276113 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.2393173375429934 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 799 0.18333130651663634 TruSeq Adapter, Index 1 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 764 0.1753005233776097 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 745 0.1709409553878524 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 709 0.16268072130199646 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.1422595870341859 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 460 0.10554743554149276 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2945094682933208E-4 0.0 16-17 0.0 0.0 0.0 2.2945094682933208E-4 0.0 18-19 0.0 0.0 0.0 5.736273670733302E-4 0.0 20-21 0.0 0.0 0.0 0.0013767056809759924 0.0 22-23 0.0 0.0 0.0 0.004703744410001308 0.0 24-25 0.0 0.0 0.0 0.010784194500978608 0.0 26-27 0.0 0.0 0.0 0.02065058521463989 0.0 28-29 0.0 0.0 0.0 0.05472405081879571 0.0 30-31 0.0 0.0 0.0 0.12952505948515797 0.0 32-33 0.0 0.0 0.0 0.2557230802412906 0.0 34-35 0.0 0.0 0.0 0.4706038919469601 0.0 36-37 0.0 0.0 0.0 0.800095451593881 0.0 38-39 0.0 0.0 0.0 1.1854583167937442 0.0 40-41 0.0 0.0 0.0 1.5928484728892234 0.0 42-43 0.0 0.0 0.0 1.9831445334459172 0.0 44-45 0.0 0.0 0.0 2.417839352214087 0.0 46-47 0.0 0.0 0.0 2.913453397365444 0.0 48-49 0.0 0.0 0.0 3.4209988917519265 0.0 50-51 0.0 0.0 0.0 3.9538987157630507 0.0 52-53 0.0 0.0 0.0 4.445267918398065 0.0 54-55 0.0 0.0 0.0 4.98310093776602 0.0 56-57 0.0 0.0 0.0 5.650344291145718 0.0 58-59 0.0 0.0 0.0 6.2684851419039385 0.0 60-61 0.0 0.0 0.0 6.934466515076075 0.0 62-63 0.0 0.0 0.0 7.5897784192206466 0.0 64-65 0.0 0.0 0.0 8.281687749384497 0.0 66-67 0.0 0.0 0.0 9.051495675996907 0.0 68-69 0.0 0.0 0.0 9.885664593194942 0.0 70-71 0.0 0.0 0.0 10.791192754856903 0.0 72-73 0.0 0.0 0.0 11.636604768449576 0.0 74-75 0.0 0.0 0.0 12.513910463651527 0.0 76-77 0.0 0.0 0.0 13.451332306922765 0.0 78-79 0.0 0.0 0.0 14.480993430819392 0.0 80-81 0.0 0.0 0.0 15.553332430826277 0.0 82-83 0.0 0.0 0.0 16.58758257365949 0.0 84-85 0.0 0.0 0.0 17.63296108741393 0.0 86-87 0.0 0.0 0.0 18.694630618393248 0.0 88-89 0.0 0.0 0.0 19.815269042707705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 135 3.110472E-10 35.14261 3 CGCAGAA 130 1.0096859E-5 25.54597 9 GGATATG 190 1.2021701E-8 24.969746 9 GGTATCA 4340 0.0 24.055805 1 CATGGGT 285 3.6379788E-12 23.305098 4 GACATAT 215 4.2398824E-8 22.173134 1 GAGTACT 3125 0.0 21.712189 12-13 GTATCAA 5490 0.0 20.927158 1 CATGGGG 635 0.0 20.919535 4 GATATAG 160 4.8431768E-5 20.856604 1 TATAGTA 235 1.14232535E-7 20.188305 2 GTCCTAA 190 7.9782985E-6 20.072521 1 GTACTTT 3360 0.0 20.052414 14-15 GTACATG 2645 0.0 19.825886 1 ACCATAC 120 0.0047922893 19.767717 3 GTCCAAG 145 5.989777E-4 19.726442 1 AGTACTT 3215 0.0 19.55476 12-13 TACATGG 2700 0.0 19.504147 2 GTATAGT 175 9.6468924E-5 19.068895 1 ACATGGG 2620 0.0 19.013224 3 >>END_MODULE