FastQCFastQC Report
Thu 26 May 2016
SRR938806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434876
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44831.0308685694312862No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42640.9805093865837617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.8754219593631288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.5668282452929111No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.531645802481627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.24880655635169563No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10720.24650705028559866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.20948500262143693No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7710.17729191769607888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.16280502947966777No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.14762828944342754No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5020.11543520451806952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA702.0385708E-433.9643331
GTCGGGT300.0039434531.67809190-91
TGTATCG650.00587269829.1975089
AGTCGGG350.00837805727.15108388-89
GTTACGG350.00839930527.1369924-25
TCTAGAC700.00843261627.111973
GGTACTC950.001233483424.9715523
GGTATCA41250.024.0920331
GAGTACT26750.022.8009912-13
GTATAGT1503.0030547E-522.1900331
GCCGTAT550.002873669821.59496194-95
GTACTTT29350.021.02372214-15
CCCGCTG1150.00374421420.6286749
GTACATG24400.020.0723651
CATGGGT2851.9826984E-919.9772424
GTATCAA52200.019.8580741
CGCCCAC600.004752641519.79880792-93
CCGTATC600.00475740419.7953894-95
ACATGGG23750.019.777473
AACCGTC1200.00479025319.7691467
Produced by FastQC (version 0.11.5)