##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938806_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 434876 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48618456755489 33.0 31.0 34.0 30.0 34.0 2 31.529091051242194 33.0 31.0 34.0 30.0 34.0 3 31.500827822183794 33.0 31.0 34.0 28.0 34.0 4 35.07906621657668 37.0 35.0 37.0 33.0 37.0 5 35.124603335203595 37.0 35.0 37.0 33.0 37.0 6 35.19793918266356 37.0 35.0 37.0 33.0 37.0 7 35.14217616056071 37.0 35.0 37.0 32.0 37.0 8 35.1173253985044 37.0 35.0 37.0 32.0 37.0 9 36.670411795546315 39.0 37.0 39.0 32.0 39.0 10-11 36.57449019950515 39.0 37.0 39.0 32.0 39.0 12-13 36.495240022443184 39.0 37.0 39.0 32.0 39.0 14-15 37.615028191944376 40.0 37.5 41.0 32.0 41.0 16-17 37.50334348182011 40.0 37.0 41.0 32.0 41.0 18-19 37.494053477313074 40.0 37.0 41.0 32.0 41.0 20-21 37.42567996394375 40.0 37.0 41.0 32.0 41.0 22-23 37.370092854054946 40.0 37.0 41.0 32.0 41.0 24-25 37.27514164957367 40.0 37.0 41.0 32.0 41.0 26-27 37.12002731813207 40.0 36.5 41.0 31.0 41.0 28-29 36.94087280052244 40.0 36.0 41.0 31.0 41.0 30-31 36.644285957376354 39.0 35.5 41.0 30.5 41.0 32-33 36.379856556811596 39.0 35.0 41.0 30.0 41.0 34-35 36.04647186784278 39.0 35.0 41.0 30.0 41.0 36-37 35.81696736540991 39.0 35.0 41.0 29.5 41.0 38-39 35.622845362816065 39.0 35.0 41.0 28.0 41.0 40-41 35.44731946577875 38.5 35.0 40.5 27.0 41.0 42-43 35.31049195632778 39.0 35.0 41.0 26.0 41.0 44-45 35.049258869194894 39.0 35.0 41.0 24.5 41.0 46-47 34.634956861266204 39.0 35.0 41.0 22.5 41.0 48-49 34.26502152337678 39.0 34.5 41.0 19.0 41.0 50-51 33.11704485876434 38.0 32.5 40.0 14.0 40.5 52-53 33.20277504392057 38.0 33.0 39.5 13.0 40.5 54-55 33.70042034970888 38.0 34.0 40.0 10.5 41.0 56-57 33.62047917107405 38.0 34.0 40.0 8.0 41.0 58-59 33.444493372823516 38.0 34.0 40.0 4.5 41.0 60-61 33.159015443482744 38.0 33.0 40.0 2.0 41.0 62-63 32.790582832807516 37.5 33.0 40.0 2.0 41.0 64-65 32.44496477156707 37.0 32.5 40.0 2.0 41.0 66-67 32.06289379041382 36.5 32.0 39.5 2.0 41.0 68-69 31.628376824658062 36.0 31.5 39.0 2.0 41.0 70-71 31.193184953871906 35.5 31.0 39.0 2.0 40.5 72-73 30.74233689603473 35.0 31.0 38.5 2.0 40.0 74-75 30.239195770748445 35.0 30.0 37.5 2.0 39.5 76-77 29.740555928586538 35.0 29.5 37.0 2.0 39.0 78-79 29.24150217533274 35.0 29.0 36.5 2.0 39.0 80-81 28.699050993846523 34.0 29.0 36.0 2.0 38.5 82-83 28.230140315860154 34.0 28.0 35.5 2.0 37.0 84-85 27.80357044306883 34.0 27.0 35.0 2.0 37.0 86-87 27.32434418086995 34.0 26.0 35.0 2.0 36.5 88-89 26.799737166456644 34.0 25.5 35.0 2.0 36.0 90-91 26.290198815294474 34.0 24.5 35.0 2.0 36.0 92-93 25.763304942098436 33.5 20.5 35.0 2.0 35.0 94-95 25.206455403379355 33.0 14.5 35.0 2.0 35.0 96-97 24.58853788206293 33.0 2.0 35.0 2.0 35.0 98-99 23.88046592591911 32.5 2.0 35.0 2.0 35.0 100-101 21.679210855508238 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3869380741296524 1101 2 0.3162146296514692 1101 3 0.3731505045449701 1101 4 0.3511390932950178 1101 5 0.22782182531949502 1101 6 0.28890384032350624 1101 7 0.48838245426667726 1101 8 0.1496001751891285 1101 9 0.31160127161292195 1101 10-11 0.32784257115586257 1101 12-13 0.2578153142114914 1101 14-15 0.3201401437770599 1101 16-17 0.3560738679242519 1101 18-19 0.38983692671163794 1101 20-21 0.6510676883329367 1101 22-23 0.5381121778866955 1101 24-25 0.41521950271754093 1101 26-27 0.5741520452175166 1101 28-29 0.5411338761249169 1101 30-31 0.3826144501081288 1101 32-33 0.1388371709419971 1101 34-35 0.07791550070297859 1101 36-37 -0.18217322169476802 1101 38-39 -0.15451810268163513 1101 40-41 0.07350653005507723 1101 42-43 -0.03633483014322536 1101 44-45 0.08841355291065867 1101 46-47 -0.6904860652362288 1101 48-49 -0.5379599431210167 1101 50-51 -0.053359902330754494 1101 52-53 -0.3964179274518145 1101 54-55 -0.46076731359026013 1101 56-57 -0.3479383849346789 1101 58-59 -0.27571956395424024 1101 60-61 -0.23714775837702717 1101 62-63 -0.4321318216480563 1101 64-65 -0.7090501240188551 1101 66-67 -0.9867668858883363 1101 68-69 -0.5009896092064956 1101 70-71 -0.25652939080645254 1101 72-73 -0.5773855915727282 1101 74-75 -0.5004711014544831 1101 76-77 -0.6034325356087678 1101 78-79 -0.6473872691534837 1101 80-81 -0.43347943269557376 1101 82-83 -0.5105699523637348 1101 84-85 -0.7222504752953967 1101 86-87 -0.5838364132662797 1101 88-89 -0.7001471442349576 1101 90-91 -0.8311487605880643 1101 92-93 -0.7731783613168801 1101 94-95 -1.2294899035543807 1101 96-97 -1.3356713150240296 1101 98-99 -0.8586636387992108 1101 100-101 -0.5037867569267469 1102 1 0.1680829361886751 1102 2 -0.1252685963410336 1102 3 -0.11788573500131605 1102 4 -0.0632846306307755 1102 5 0.08416134276638587 1102 6 0.08420327317941201 1102 7 -0.15580672193249967 1102 8 0.020142326491637164 1102 9 0.1899536699595572 1102 10-11 0.2218635266494644 1102 12-13 0.39039726172663336 1102 14-15 0.27681129632920687 1102 16-17 0.441445875998852 1102 18-19 0.08530938657898446 1102 20-21 0.2335679286482275 1102 22-23 0.16483043223640692 1102 24-25 0.18708819444108116 1102 26-27 0.0061187855808739755 1102 28-29 0.13485684065437908 1102 30-31 0.22040162688405474 1102 32-33 0.08402125245694947 1102 34-35 0.09581899008103534 1102 36-37 -0.017929734239224615 1102 38-39 -0.044778123829388505 1102 40-41 -0.10048174255859266 1102 42-43 0.15344215726425148 1102 44-45 0.23981218791979586 1102 46-47 0.23976242077741716 1102 48-49 0.07423125172662282 1102 50-51 0.14231682553619862 1102 52-53 -0.08394796686351924 1102 54-55 -0.22612346085546875 1102 56-57 -0.3213234661612461 1102 58-59 -0.20548741938055315 1102 60-61 -0.24861079788485796 1102 62-63 -0.46780662699266884 1102 64-65 -0.29564774009114103 1102 66-67 -0.13427241314008853 1102 68-69 -0.4222743347664313 1102 70-71 -0.48969530448519905 1102 72-73 -0.5189288963886476 1102 74-75 -0.8987937007047009 1102 76-77 -0.7934498383099111 1102 78-79 -1.0039911334234048 1102 80-81 -0.705023698772667 1102 82-83 -0.6418782288224492 1102 84-85 -0.9052145241274623 1102 86-87 -0.6015476504094117 1102 88-89 -0.70521779693129 1102 90-91 -0.8977521442274004 1102 92-93 -1.523346582022448 1102 94-95 -1.2367474254230686 1102 96-97 -1.7315907613375892 1102 98-99 -1.944071694167853 1102 100-101 -1.704726389724982 1103 1 0.3882731718399022 1103 2 0.6290528347130255 1103 3 0.7032209220879189 1103 4 0.6828399841478259 1103 5 0.8364668783689524 1103 6 0.5726002887272941 1103 7 0.6193224575748815 1103 8 0.8241080794401725 1103 9 0.8003297387317119 1103 10-11 0.7135971967377515 1103 12-13 0.8140075682817738 1103 14-15 0.85899089639409 1103 16-17 0.898466173980637 1103 18-19 1.2382915215744674 1103 20-21 0.9591976063366658 1103 22-23 0.9065843771352675 1103 24-25 1.0339988925897003 1103 26-27 1.055522706149958 1103 28-29 0.8556350458122921 1103 30-31 0.9385585886747236 1103 32-33 1.2245285861455741 1103 34-35 1.309312745439378 1103 36-37 1.1914916805949858 1103 38-39 1.1675860349397595 1103 40-41 0.9797572684940761 1103 42-43 1.0930869425829641 1103 44-45 1.1169468358332253 1103 46-47 0.8860162150633997 1103 48-49 1.1069808636088183 1103 50-51 1.0333889575194277 1103 52-53 0.878810102511089 1103 54-55 1.0240474268336612 1103 56-57 1.0524137027800577 1103 58-59 1.156420845790791 1103 60-61 0.9096775446783383 1103 62-63 0.886492791409907 1103 64-65 0.5019662043442992 1103 66-67 0.5288702983551445 1103 68-69 0.6183303180129549 1103 70-71 0.6511480117786768 1103 72-73 0.687161656708188 1103 74-75 0.5449842495698753 1103 76-77 0.3585880697930648 1103 78-79 0.12414856581426292 1103 80-81 0.39359587626730175 1103 82-83 0.328485790919423 1103 84-85 0.22965351581970594 1103 86-87 0.10516852541374888 1103 88-89 0.29378584094900617 1103 90-91 0.016372447379986 1103 92-93 -0.321221202593847 1103 94-95 -0.4991353346846026 1103 96-97 -0.13751564026800622 1103 98-99 -0.30699949267099313 1103 100-101 0.2315035382125501 1104 1 -0.09418311491232956 1104 2 -0.17920414299502951 1104 3 -0.013409305282166883 1104 4 -0.004794576995088562 1104 5 -0.002860858333484373 1104 6 0.04053092610900677 1104 7 0.11567018303955479 1104 8 0.15588926563663108 1104 9 0.3084047981194189 1104 10-11 0.17528308443795737 1104 12-13 0.29062244107803537 1104 14-15 0.12075578226399131 1104 16-17 0.12205046558401733 1104 18-19 -0.03517288196153601 1104 20-21 0.13788367762416698 1104 22-23 -0.06643385363953058 1104 24-25 -0.07829811827532751 1104 26-27 0.02906388255509995 1104 28-29 -0.009464452938757972 1104 30-31 -0.06983079441632611 1104 32-33 0.04483834798278252 1104 34-35 -0.10757227884420928 1104 36-37 0.29982209322134423 1104 38-39 0.26996376858241433 1104 40-41 -0.05501305267242884 1104 42-43 0.02127093043282713 1104 44-45 0.3353267057644018 1104 46-47 0.14577833043410493 1104 48-49 0.020043934189793333 1104 50-51 0.2269344732606413 1104 52-53 0.2116649962251671 1104 54-55 0.32181767567789876 1104 56-57 0.2979338946009662 1104 58-59 0.43433028718471434 1104 60-61 0.612191021654823 1104 62-63 0.7846658196545349 1104 64-65 0.592099221684947 1104 66-67 0.44583598978384487 1104 68-69 0.20567336478320897 1104 70-71 0.4342896911017391 1104 72-73 0.25612372089698354 1104 74-75 0.24523904648338757 1104 76-77 0.32775096735691633 1104 78-79 0.4426419260737333 1104 80-81 0.3650619599052227 1104 82-83 0.45199553495422506 1104 84-85 0.38671522896733634 1104 86-87 0.34348708831464947 1104 88-89 0.3735044516938686 1104 90-91 0.3767431824523939 1104 92-93 0.32188787607609015 1104 94-95 0.2997197481800242 1104 96-97 0.2549550553053166 1104 98-99 0.28099167288580773 1104 100-101 0.376114694756879 1105 1 -0.6040089372381523 1105 2 -0.4525789163698022 1105 3 -0.5286417580146221 1105 4 -0.3250160222165377 1105 5 -0.15448932246194857 1105 6 -0.20687907130098893 1105 7 -0.27069345332408545 1105 8 -0.019793847546480947 1105 9 -0.24260206538744455 1105 10-11 -0.061791183886306555 1105 12-13 -0.06930698135139579 1105 14-15 -0.2920825368243314 1105 16-17 -0.2646288273452768 1105 18-19 -0.10698879752744972 1105 20-21 -0.1071143798738845 1105 22-23 -0.5203884638441423 1105 24-25 -0.16571879944600454 1105 26-27 -0.04993119643997801 1105 28-29 0.034533216058918015 1105 30-31 -0.10213298296852003 1105 32-33 -0.29876596437152614 1105 34-35 -0.2211196111415461 1105 36-37 0.05298675263599506 1105 38-39 -0.10707585845722178 1105 40-41 -0.10332469473407002 1105 42-43 -0.3554125712506746 1105 44-45 -0.18284378115608035 1105 46-47 -0.26069829479251894 1105 48-49 -0.14668748254162978 1105 50-51 -0.06975841718224274 1105 52-53 -0.12713162382145526 1105 54-55 -0.022442231082010267 1105 56-57 -0.10458488291780554 1105 58-59 -0.08353263692820434 1105 60-61 -0.20249429303049027 1105 62-63 0.04774533273405268 1105 64-65 0.17260783344356412 1105 66-67 0.23151327546113265 1105 68-69 0.17784702445687017 1105 70-71 -0.1627220934100464 1105 72-73 -0.20343986366660616 1105 74-75 -0.5731127406184022 1105 76-77 -0.5050643654584164 1105 78-79 -0.23239368646187586 1105 80-81 -0.1726387652955026 1105 82-83 -0.5819739677652755 1105 84-85 -0.4504545457024385 1105 86-87 0.02602561460317432 1105 88-89 -0.055035758096341425 1105 90-91 0.29201772272693205 1105 92-93 0.3871397538279666 1105 94-95 0.5610995720598488 1105 96-97 0.803412385012642 1105 98-99 1.159488176382311 1105 100-101 1.444118450260639 1106 1 0.23685689973045854 1106 2 0.3647592069729413 1106 3 0.20112365982816272 1106 4 0.2228092279759224 1106 5 0.4020552417327323 1106 6 0.4789262490308914 1106 7 0.5793835289699913 1106 8 0.5064817062983593 1106 9 0.2690361605983611 1106 10-11 0.38608806083185243 1106 12-13 0.6085916158601421 1106 14-15 0.7163026526376441 1106 16-17 0.6122877110175722 1106 18-19 0.6887702265081259 1106 20-21 0.5987330668800226 1106 22-23 0.5548644016079152 1106 24-25 0.4897827615838466 1106 26-27 0.35854540949620883 1106 28-29 0.43743929766014844 1106 30-31 0.35717599402233446 1106 32-33 0.3960714105322438 1106 34-35 0.4684786909711889 1106 36-37 0.4860950196790199 1106 38-39 0.3684822631748048 1106 40-41 0.5146693819060815 1106 42-43 0.5001707834666362 1106 44-45 0.08983124664308662 1106 46-47 0.3086793247438777 1106 48-49 0.3794651134723992 1106 50-51 0.18522137496556468 1106 52-53 0.1728346964248928 1106 54-55 -0.026435687587010648 1106 56-57 0.1943523337445754 1106 58-59 0.08993680537784599 1106 60-61 0.04923780858613469 1106 62-63 0.29398175391273185 1106 64-65 0.28195209398585064 1106 66-67 0.5725876061428465 1106 68-69 0.5177719667201508 1106 70-71 0.2956165859159334 1106 72-73 0.20175430278622386 1106 74-75 0.275176007136281 1106 76-77 0.5081936962984912 1106 78-79 0.4291996331857142 1106 80-81 0.4496294324634569 1106 82-83 0.1566150109362887 1106 84-85 0.39392895442586706 1106 86-87 0.4512057745552731 1106 88-89 0.30343710564499915 1106 90-91 0.08967190115478374 1106 92-93 0.4063799813534139 1106 94-95 0.7936876740186207 1106 96-97 0.8740730427835786 1106 98-99 1.0023480686070982 1106 100-101 1.0374006759042942 1107 1 -0.22955607528528787 1107 2 -0.15880787259875717 1107 3 -0.04476128663414869 1107 4 -0.11440996161047678 1107 5 -0.12978393525656173 1107 6 -0.08044809487001459 1107 7 -0.060087392718024546 1107 8 -0.15384266909529742 1107 9 0.12174978646441303 1107 10-11 -0.2107600391051605 1107 12-13 -0.13125984330425666 1107 14-15 -0.002204590696386788 1107 16-17 -0.06772226168870787 1107 18-19 0.04303224624359103 1107 20-21 -0.03994266270216684 1107 22-23 0.16523864053295512 1107 24-25 -0.16422469170189657 1107 26-27 -0.22728809879688328 1107 28-29 -0.06281526878957777 1107 30-31 0.017290417704877825 1107 32-33 0.10331736896180388 1107 34-35 0.09199065821872665 1107 36-37 -0.14295179955255577 1107 38-39 -0.1358637372450957 1107 40-41 -0.49711678227615863 1107 42-43 -0.016229069567167187 1107 44-45 0.06839780133550732 1107 46-47 0.36390187355765136 1107 48-49 0.3887502278960824 1107 50-51 0.2603552191813918 1107 52-53 0.42419413375430537 1107 54-55 0.38361837497860307 1107 56-57 0.6795481137151853 1107 58-59 0.6548722452266773 1107 60-61 0.8065966160604177 1107 62-63 0.814706612195323 1107 64-65 0.8470817391674643 1107 66-67 0.7735166424645001 1107 68-69 0.651710660820509 1107 70-71 0.47178969110173696 1107 72-73 0.6467705740438312 1107 74-75 0.4495514007957411 1107 76-77 0.09931273891868742 1107 78-79 0.38183190276371093 1107 80-81 0.6342635916068566 1107 82-83 0.3702356281943189 1107 84-85 0.9085101007622107 1107 86-87 0.7631257829533418 1107 88-89 0.8012142419036579 1107 90-91 0.6467315274407355 1107 92-93 1.0999764541646684 1107 94-95 1.1706113565716336 1107 96-97 1.4949317452820026 1107 98-99 1.5746396915338252 1107 100-101 1.952367608509796 1108 1 0.3640327985274645 1108 2 0.25582351591322805 1108 3 -0.08001682711357105 1108 4 0.2141630822610736 1108 5 0.21239155094878015 1108 6 0.2294116970793283 1108 7 -0.08039494586139995 1108 8 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-2.1180165273271108 2316 90-91 -2.1717999410907325 2316 92-93 -1.8962648045765889 2316 94-95 -1.933059972099695 2316 96-97 -1.8937920309417713 2316 98-99 -2.640832336438205 2316 100-101 -2.7656393844971987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3810.0 3 678.0 4 539.0 5 566.0 6 604.0 7 753.0 8 913.0 9 1145.0 10 1326.0 11 1750.0 12 2527.0 13 3211.0 14 3523.0 15 4409.0 16 4932.0 17 6269.0 18 5492.0 19 3563.0 20 3488.0 21 3643.0 22 3825.0 23 4096.0 24 4696.0 25 5375.0 26 5951.0 27 6781.0 28 7895.0 29 9272.0 30 10901.0 31 13008.0 32 15631.0 33 19509.0 34 24817.0 35 36923.0 36 46495.0 37 71497.0 38 79690.0 39 15359.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.856277207998225 22.608232494650633 15.355686932782408 20.17980336456873 2 15.336226395971824 24.02854365622308 39.350258671126035 21.28497127667906 3 17.673461581885753 29.05868880486451 32.852919693733426 20.414929919516315 4 11.627564158670346 20.569470100746734 40.12156864551615 27.681397095066774 5 11.209102266807431 39.33305057788146 35.86622286354437 13.591624291766745 6 25.244300049539742 40.086636942821926 19.83893823661563 14.830124771022707 7 22.736996924722558 36.81342241033514 24.241669778828996 16.2079108861133 8 23.354228000082994 34.940047906345164 22.917584026300077 18.78814006727176 9 24.787560853164827 18.198147576696865 24.261817143436147 32.75247442670216 10-11 21.771766581867787 28.96503889687719 29.728804888839733 19.534389632415294 12-13 23.51855351274618 28.18066802785677 29.698179907543544 18.602598551853507 14-15 21.183240175849388 29.182152922626496 29.291684470822112 20.342922430702004 16-17 17.943839232278087 32.08204901123054 30.49231482410409 19.481796932387287 18-19 17.70254088734558 30.37758920534892 33.927478624253624 17.992391283051877 20-21 19.033404570302825 29.301666484253943 33.76931894768979 17.895609997753443 22-23 18.780097603256824 29.42240264455494 33.58517924282556 18.212320509362677 24-25 18.581524156799652 29.992186293514163 33.530596768951696 17.89569278073449 26-27 18.175566767776647 29.682374673046326 34.12571404836434 18.01634451081269 28-29 17.82580561069772 29.96086283999903 34.160292796248 18.053038753055247 30-31 18.43209295674596 29.75658359049583 33.73060902536567 18.080714427392532 32-33 17.943235777265283 30.126416628843817 33.776878221181875 18.15346937270903 34-35 18.34967084576196 29.807123116955047 33.70066292413586 18.14254311314713 36-37 18.470517750301006 29.623776503087385 33.40090295486265 18.504802791748965 38-39 18.011167175244786 30.17203174266101 33.40584995637462 18.41095112571959 40-41 17.98648628382034 29.93806117281152 33.76317887273411 18.312273670634028 42-43 17.785851041362523 30.093513166049053 33.13849019456232 18.9821455980261 44-45 18.140184434980323 29.76651316320995 33.12812344909572 18.96517895271401 46-47 18.43352120121562 29.995186919501514 32.63961107539454 18.931680803888323 48-49 18.185197670713517 29.96277944279995 32.62929063898368 19.22273224750285 50-51 17.909164159738463 30.22512715493855 32.452041289857846 19.413667395465133 52-53 18.10972174764275 30.503749434352578 32.04704339554685 19.33948542245782 54-55 18.049859347098117 30.010608126124733 32.003296714712974 19.936235812064176 56-57 18.3380110048377 30.268150596403114 31.74715646811182 19.646681930647365 58-59 18.46659182762253 29.611571516090585 31.95642567802213 19.965410978264757 60-61 18.37956106720553 29.899470826240005 32.08825252706998 19.632715579484483 62-63 18.09439611095594 29.674043262383513 32.278165445954336 19.953395180706206 64-65 18.173536753482765 29.616151073405362 32.054375711504846 20.155936461607023 66-67 18.190310248034464 29.58885698153338 31.519117278583543 20.701715491848613 68-69 18.24356391055582 29.266352199442274 31.903142555267515 20.586941334734387 70-71 18.431332699775638 29.38814723634512 31.79320307294728 20.387316990931964 72-73 18.561020667359426 28.901858907747375 31.83396836393026 20.703152060962935 74-75 18.27400325350433 29.55308765411716 31.41894583227019 20.753963260108318 76-77 18.408455240256586 29.04641909355041 31.66940435390389 20.87572131228912 78-79 18.503336829994197 28.8542607908916 31.63150964582172 21.01089273329248 80-81 18.64924974348972 28.85472342478449 31.43898517722581 21.05704165449998 82-83 18.72004709358696 28.661641811717992 31.533080936331316 21.08523015836373 84-85 18.593213080731307 28.43250306172036 31.432815870850227 21.54146798669811 86-87 18.728250042417827 28.476963368310248 31.4493277207141 21.345458868557827 88-89 19.20029920385456 27.81383794740615 31.438567977107212 21.54729487163208 90-91 19.142283810214362 27.675571576028098 31.578406537707508 21.603738076050035 92-93 19.639611971523642 27.742050196161745 31.413588385931774 21.20474944638284 94-95 19.658975309369 27.134406497643866 31.401660668077692 21.804957524909444 96-97 19.758228409374336 26.89082519653035 31.281234700193068 22.069711693902246 98-99 19.94222055950451 26.83187614600509 30.976430976430976 22.249472318059425 100-101 20.395748394335502 26.157094243617745 31.635630745647042 21.81152661639971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3999.0 1 2499.5 2 1079.5 3 1108.5 4 908.0 5 855.5 6 1340.5 7 2083.0 8 2724.0 9 2891.5 10 2237.5 11 1538.0 12 1329.0 13 1246.5 14 1238.0 15 1202.0 16 1148.0 17 1183.5 18 1221.5 19 1319.0 20 1499.0 21 1690.0 22 1935.0 23 2180.0 24 2532.0 25 3130.5 26 4081.0 27 5292.0 28 6008.5 29 6630.5 30 7819.0 31 8970.0 32 10473.0 33 12537.0 34 14517.5 35 15964.0 36 17357.0 37 18975.0 38 18667.5 39 17283.5 40 16511.5 41 15884.5 42 15241.5 43 14934.0 44 14925.0 45 14499.0 46 13709.5 47 13540.5 48 14816.0 49 14374.0 50 12093.5 51 10161.0 52 8459.0 53 7372.5 54 6447.0 55 5586.0 56 4815.5 57 4142.0 58 3575.0 59 2978.5 60 2513.5 61 2100.5 62 1670.5 63 1322.5 64 1008.0 65 737.0 66 569.0 67 477.5 68 379.5 69 283.0 70 210.0 71 162.5 72 141.5 73 119.0 74 91.5 75 77.0 76 71.0 77 53.0 78 31.0 79 20.0 80 18.5 81 15.0 82 10.0 83 6.5 84 6.0 85 6.0 86 5.0 87 3.0 88 0.5 89 0.5 90 1.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27134171579944627 2 0.16878374525151998 3 0.2012067807834877 4 0.16510453554576476 5 0.24144813694018524 6 0.20258648442314592 7 0.251565963631012 8 0.25593502515659633 9 0.14578868459054994 10-11 0.20879515080160782 12-13 0.21661347142633763 14-15 0.17349773268701882 16-17 0.29468170237033087 18-19 0.23719405071790578 20-21 0.20201160790662165 22-23 0.24811670453186657 24-25 0.23523947056172334 26-27 0.3494099467434395 28-29 0.3220458245568852 30-31 0.2984758873793909 32-33 0.19200875651909968 34-35 0.3606775264673148 36-37 0.4006889320174027 38-39 0.3775788960531278 40-41 0.336762663379906 42-43 0.37286490861762894 44-45 0.3819479575787121 46-47 0.3866619450142109 48-49 0.4097719809784859 50-51 0.40080390732070753 52-53 0.4010338579273172 54-55 0.39494016685216016 56-57 0.3706803778548368 58-59 0.3452708358244649 60-61 0.3150323310552893 62-63 0.3814880563654927 64-65 0.41736035099660596 66-67 0.42575354813786 68-69 0.42966270845022486 70-71 0.430352560270054 72-73 0.4198898076693126 74-75 0.4149458696272041 76-77 0.39654982109842807 78-79 0.36918569891187375 80-81 0.3811431304555781 82-83 0.3899962288100516 84-85 0.391950808966234 86-87 0.3875817474406497 88-89 0.39700972231164744 90-91 0.4182801534230447 92-93 0.41736035099660596 94-95 0.4271332517775182 96-97 0.4286279307204812 98-99 0.4254086222279454 100-101 0.3959749445819038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 434876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.70263674267664 #Duplication Level Percentage of deduplicated Percentage of total 1 87.54278532170426 52.26535111503834 2 7.143013083542492 8.52913430749848 3 1.7685594137551823 3.167629807118004 4 0.7809361654363338 1.8649579281705706 5 0.4776068751691185 1.4257194887013394 6 0.3428315579632751 1.2280768781404379 7 0.2545868210719543 1.0639653148552188 8 0.20261958644661252 0.9677538853258779 9 0.1519747207228729 0.8165962390848671 >10 1.1650173423959727 13.780978070921657 >50 0.11415069635264885 4.645548088885442 >100 0.051290684506390356 4.944399326149121 >500 0.0019282212220447504 0.8136673908549938 >1k 0.0026995097108626505 4.486222159255675 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4483 1.0308685694312862 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4264 0.9805093865837617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3807 0.8754219593631288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.5668282452929111 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2312 0.531645802481627 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.24880655635169563 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1072 0.24650705028559866 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.20948500262143693 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 771 0.17729191769607888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.16280502947966777 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.14762828944342754 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 502 0.11543520451806952 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.2995060660970025E-4 0.0 10-11 0.0 0.0 0.0 4.599012132194005E-4 0.0 12-13 0.0 0.0 0.0 4.599012132194005E-4 0.0 14-15 1.1497530330485012E-4 0.0 0.0 5.748765165242506E-4 0.0 16-17 2.2995060660970025E-4 0.0 0.0 0.001034777729743651 0.0 18-19 2.2995060660970025E-4 0.0 0.0 0.0013797036396582013 0.0 20-21 2.2995060660970025E-4 0.0 0.0 0.0025294566727067026 0.0 22-23 2.2995060660970025E-4 0.0 0.0 0.006323641681766757 0.0 24-25 2.2995060660970025E-4 0.0 0.0 0.011497530330485011 0.0 26-27 2.2995060660970025E-4 0.0 0.0 0.02540954203037188 0.0 28-29 2.2995060660970025E-4 0.0 0.0 0.058522429382168714 0.0 30-31 2.2995060660970025E-4 0.0 0.0 0.12969214212787095 0.0 32-33 2.2995060660970025E-4 0.0 0.0 0.2489215316550005 0.0 34-35 2.2995060660970025E-4 0.0 0.0 0.43656122664851593 0.0 36-37 2.2995060660970025E-4 0.0 0.0 0.717215942015655 0.0 38-39 4.599012132194005E-4 0.0 0.0 1.0869765174440529 0.0 40-41 4.599012132194005E-4 0.0 0.0 1.468924475022765 0.0 42-43 4.599012132194005E-4 0.0 0.0 1.8450086921329298 0.0 44-45 4.599012132194005E-4 0.0 0.0 2.254320771898196 0.0 46-47 4.599012132194005E-4 0.0 0.0 2.724569762415033 0.0 48-49 4.599012132194005E-4 0.0 0.0 3.1947037776285656 0.0 50-51 4.599012132194005E-4 0.0 0.0 3.681394236517996 0.0 52-53 4.599012132194005E-4 0.0 0.0 4.135891610482068 0.0 54-55 4.599012132194005E-4 0.0 0.0 4.639483438957312 0.0 56-57 4.599012132194005E-4 0.0 0.0 5.252876682088687 0.0 58-59 4.599012132194005E-4 1.1497530330485012E-4 0.0 5.8905297142173865 0.0 60-61 4.599012132194005E-4 2.2995060660970025E-4 0.0 6.533816536208023 0.0 62-63 4.599012132194005E-4 2.2995060660970025E-4 0.0 7.183312024577121 0.0 64-65 4.599012132194005E-4 2.2995060660970025E-4 0.0 7.834992043709011 0.0 66-67 4.599012132194005E-4 2.2995060660970025E-4 0.0 8.5838261941335 0.0 68-69 4.599012132194005E-4 2.2995060660970025E-4 0.0 9.404634884426825 0.0 70-71 4.599012132194005E-4 2.2995060660970025E-4 0.0 10.306156237640156 0.0 72-73 4.599012132194005E-4 2.2995060660970025E-4 0.0 11.160192790588582 0.0 74-75 4.599012132194005E-4 2.2995060660970025E-4 0.0 12.046997304978891 0.0 76-77 4.599012132194005E-4 2.2995060660970025E-4 0.0 12.976457656895299 0.0 78-79 4.599012132194005E-4 2.2995060660970025E-4 0.0 13.985940819911884 0.0 80-81 4.599012132194005E-4 2.2995060660970025E-4 0.0 15.032446030592629 0.0 82-83 4.599012132194005E-4 2.2995060660970025E-4 0.0 16.069408291099073 0.0 84-85 4.599012132194005E-4 2.2995060660970025E-4 0.0 17.103381193719592 0.0 86-87 4.599012132194005E-4 2.2995060660970025E-4 0.0 18.18058481038273 0.0 88-89 4.599012132194005E-4 2.2995060660970025E-4 0.0 19.247670600355043 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAA 70 2.0385708E-4 33.964333 1 GTCGGGT 30 0.00394345 31.678091 90-91 TGTATCG 65 0.005872698 29.197508 9 AGTCGGG 35 0.008378057 27.151083 88-89 GTTACGG 35 0.008399305 27.13699 24-25 TCTAGAC 70 0.008432616 27.11197 3 GGTACTC 95 0.0012334834 24.971552 3 GGTATCA 4125 0.0 24.092033 1 GAGTACT 2675 0.0 22.80099 12-13 GTATAGT 150 3.0030547E-5 22.190033 1 GCCGTAT 55 0.0028736698 21.594961 94-95 GTACTTT 2935 0.0 21.023722 14-15 CCCGCTG 115 0.003744214 20.628674 9 GTACATG 2440 0.0 20.072365 1 CATGGGT 285 1.9826984E-9 19.977242 4 GTATCAA 5220 0.0 19.858074 1 CGCCCAC 60 0.0047526415 19.798807 92-93 CCGTATC 60 0.004757404 19.79538 94-95 ACATGGG 2375 0.0 19.77747 3 AACCGTC 120 0.004790253 19.769146 7 >>END_MODULE