FastQCFastQC Report
Thu 26 May 2016
SRR938806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434876
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45441.044895556434478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.9494660546914523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.8669137869185699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.5863740468547356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21320.49025469329188087No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15220.3499848232599638No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11880.27318132065232387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.25639492636981576No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT7720.17752186830268857TruSeq Adapter, Index 1 (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7280.1674040416118618No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.16510453554576476No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.16349488129949685No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.14256937609801415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4800.11037629117265611No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA550.002562704334.587031
CATTCCG1151.0438998E-733.0071649
GTATGGT700.00833686127.1755221
GGTATCA38850.026.3186381
CCTTTAG1658.2878614E-825.9402711
TTAGGCA1501.0645526E-625.3142244
GTGCAGG1801.9227446E-723.7785821
CTATGGG1000.00165926923.7320843
CCCTATA1000.00165926923.7320842
TCTAGTC1050.002203683422.6019863
GCATTCC2103.473906E-822.5941898
GAGTACT27800.022.44383212-13
GTACATG25700.022.3907261
GTACCCA1703.1088766E-622.379841
TACTGTA1503.0534335E-522.1423057
TATGCTA1100.002887133521.5746235
ACTATAC1353.8100724E-421.0951863
TAGGGTA1150.00373590620.6365975
AGGGTAG1150.003743382620.6294776
GTATCAA49950.020.565261
Produced by FastQC (version 0.11.5)