##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938806_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 434876 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.957254481737323 31.0 31.0 34.0 30.0 34.0 2 32.15517756785842 33.0 31.0 34.0 30.0 34.0 3 32.20059051315777 34.0 31.0 34.0 30.0 34.0 4 35.87158638324488 37.0 35.0 37.0 35.0 37.0 5 35.7809559506618 37.0 35.0 37.0 35.0 37.0 6 35.81530137326502 37.0 35.0 37.0 33.0 37.0 7 35.74067320339591 37.0 35.0 37.0 33.0 37.0 8 35.747127916923446 37.0 35.0 37.0 33.0 37.0 9 37.344753446959594 39.0 37.0 39.0 34.0 39.0 10-11 37.29923127512211 39.0 37.0 39.0 33.5 39.0 12-13 37.238010375371374 39.0 37.0 39.0 33.0 39.0 14-15 38.397427082662645 40.0 38.0 41.0 33.0 41.0 16-17 38.330654025515315 40.0 38.0 41.0 33.0 41.0 18-19 38.44827606030225 40.0 38.0 41.0 33.5 41.0 20-21 38.45545626799364 40.0 38.0 41.0 34.0 41.0 22-23 38.34193540227559 40.0 38.0 41.0 34.0 41.0 24-25 38.249249211269415 40.0 37.5 41.0 33.0 41.0 26-27 38.04087601983094 40.0 37.0 41.0 33.0 41.0 28-29 37.83452754348366 40.0 37.0 41.0 33.0 41.0 30-31 37.529710768127 39.5 36.0 41.0 32.5 41.0 32-33 37.246441514362715 39.0 35.5 41.0 32.0 41.0 34-35 37.11309200783671 39.0 35.0 41.0 31.5 41.0 36-37 36.92040604678115 39.5 35.0 41.0 31.0 41.0 38-39 36.739373982468564 39.0 35.0 41.0 31.0 41.0 40-41 36.52530146524526 39.0 35.0 41.0 30.5 41.0 42-43 36.25824487899999 39.0 35.0 41.0 30.0 41.0 44-45 35.92244915792088 39.0 35.0 41.0 29.5 41.0 46-47 35.480538130409585 39.0 35.0 41.0 26.5 41.0 48-49 35.089720977933936 39.0 35.0 41.0 24.5 41.0 50-51 34.823983158417576 39.0 35.0 40.5 24.0 41.0 52-53 34.63244235138292 39.0 35.0 40.0 23.0 41.0 54-55 34.36914545755572 38.0 34.0 40.0 23.0 41.0 56-57 34.085261085918745 38.0 34.0 40.0 21.5 41.0 58-59 33.77536585141512 38.0 33.5 40.0 19.5 41.0 60-61 33.39765818302229 38.0 33.0 40.0 16.5 41.0 62-63 33.02354119335167 37.0 33.0 40.0 11.5 41.0 64-65 32.6204147849042 37.0 32.5 39.5 7.5 41.0 66-67 32.17507174458926 36.0 32.0 39.0 3.5 40.5 68-69 31.835643953678748 36.0 31.5 39.0 2.0 40.0 70-71 31.426049034667354 35.0 31.0 38.5 2.0 40.0 72-73 30.93989666019739 35.0 31.0 38.0 2.0 40.0 74-75 30.442938906722837 35.0 30.0 37.0 2.0 39.0 76-77 28.474892843017322 32.5 27.0 35.5 2.0 37.5 78-79 29.372746484055224 34.0 29.0 36.0 2.0 39.0 80-81 29.381118985641884 34.0 29.0 36.0 2.0 37.5 82-83 29.029729164175535 34.0 29.0 35.5 2.0 37.0 84-85 28.6120721768964 34.0 29.0 35.0 2.0 37.0 86-87 28.03730028789816 34.0 28.5 35.0 2.0 36.0 88-89 27.438185827684215 34.0 26.5 35.0 2.0 36.0 90-91 26.928414076656335 33.0 25.5 35.0 2.0 35.0 92-93 26.627122444099008 33.0 25.0 35.0 2.0 35.0 94-95 26.301562974273125 33.0 25.0 35.0 2.0 35.0 96-97 25.74126992522006 33.0 23.5 35.0 2.0 35.0 98-99 25.001037077235807 32.5 10.5 35.0 2.0 35.0 100-101 21.77069440484184 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2843553740435958 1101 2 0.3450157214534002 1101 3 0.1704987040711714 1101 4 0.14221771216062962 1101 5 0.2241848074026933 1101 6 0.2791332808452296 1101 7 0.36194099322279527 1101 8 0.24681564282330015 1101 9 0.22099257066035705 1101 10-11 0.19223808721449132 1101 12-13 0.22576834336032192 1101 14-15 0.48798563050807786 1101 16-17 0.41302733913025236 1101 18-19 0.37018808786214663 1101 20-21 0.19245038624902122 1101 22-23 0.2332484103563246 1101 24-25 0.27900822992634744 1101 26-27 0.255019198802124 1101 28-29 0.2459777686745923 1101 30-31 0.5478097125774966 1101 32-33 0.6271993773137297 1101 34-35 0.31481895776809665 1101 36-37 0.4495908854908919 1101 38-39 0.721750968761043 1101 40-41 0.6612175050066043 1101 42-43 0.6428275389164781 1101 44-45 0.785489866163843 1101 46-47 1.0251129188170651 1101 48-49 1.201122156718803 1101 50-51 0.8113942373089884 1101 52-53 0.8426116314417129 1101 54-55 0.8500101759828311 1101 56-57 0.7991942566225703 1101 58-59 0.8858871983164889 1101 60-61 1.1201051268664983 1101 62-63 1.0351810385154465 1101 64-65 1.0387715466688547 1101 66-67 1.1323977935384448 1101 68-69 1.1093905122544676 1101 70-71 0.81541943217481 1101 72-73 0.7544641154977505 1101 74-75 0.5694364064600599 1101 76-77 0.539990558052942 1101 78-79 0.4094171790344028 1101 80-81 0.7912011163498214 1101 82-83 0.9468631456619363 1101 84-85 1.1843062415298569 1101 86-87 1.0204058035528796 1101 88-89 0.8784267724914017 1101 90-91 0.5690162484990573 1101 92-93 0.10043228308374097 1101 94-95 0.03341512374286282 1101 96-97 0.014652626306649097 1101 98-99 -0.18688872644300858 1101 100-101 0.5493326727224286 1102 1 0.06799951515673186 1102 2 -0.2987649187465422 1102 3 -0.10684917634793578 1102 4 0.03470064977477705 1102 5 -0.3223642649802727 1102 6 -0.31759362485504283 1102 7 -0.07283743607644766 1102 8 0.020751046072362556 1102 9 -0.016126529599183925 1102 10-11 0.017024206603132086 1102 12-13 -0.10154108661179606 1102 14-15 -0.010139910201331759 1102 16-17 -0.1640057282541605 1102 18-19 -0.19505751436798846 1102 20-21 -0.0769310421850875 1102 22-23 0.029340883681328478 1102 24-25 0.003054178210490477 1102 26-27 -0.21194946408358106 1102 28-29 -0.17312008771298792 1102 30-31 -0.1942478669668617 1102 32-33 -0.052616541171317976 1102 34-35 0.18622624412780198 1102 36-37 -0.455052005982715 1102 38-39 -0.23440350590972514 1102 40-41 -0.5195116115943819 1102 42-43 -0.4147279739163636 1102 44-45 -0.3474645700564807 1102 46-47 -1.0431005772898274 1102 48-49 -0.98764550645528 1102 50-51 -0.7527459145702764 1102 52-53 -0.7391796787572673 1102 54-55 -0.5718993693406276 1102 56-57 -0.5766588626699374 1102 58-59 -0.4567062843327463 1102 60-61 -1.0022994679619188 1102 62-63 -1.0526542013192142 1102 64-65 -1.023926713448418 1102 66-67 -1.0511189804690488 1102 68-69 -1.2755825724326435 1102 70-71 -1.2427248531275055 1102 72-73 -1.0584023653250902 1102 74-75 -1.8511033416895124 1102 76-77 -1.605964935554674 1102 78-79 -1.400853896616848 1102 80-81 -1.212439660349208 1102 82-83 -1.2229796881360073 1102 84-85 -1.0888241054854255 1102 86-87 -0.9881133736075576 1102 88-89 -1.27152997075574 1102 90-91 -0.9316085712644728 1102 92-93 -1.016433044225689 1102 94-95 -1.0872471852064756 1102 96-97 -1.1278663586015476 1102 98-99 -1.0265143152154792 1102 100-101 -0.39605567679213394 1103 1 0.18886635020087894 1103 2 0.16614807335207615 1103 3 0.1821305951839136 1103 4 0.1957811233943545 1103 5 -0.1680208212747374 1103 6 0.03904608596823067 1103 7 0.2160786146221696 1103 8 0.27483497220254094 1103 9 0.13793885956132357 1103 10-11 0.2987948347746894 1103 12-13 0.37917756853105544 1103 14-15 0.14657278994001643 1103 16-17 0.23236971489527747 1103 18-19 0.018392794967695636 1103 20-21 0.2681433374057107 1103 22-23 0.35164085819299373 1103 24-25 0.3093196739585693 1103 26-27 0.5022249456297914 1103 28-29 0.058172054928995465 1103 30-31 0.4611701416991707 1103 32-33 0.39397671137172097 1103 34-35 0.43662594753627815 1103 36-37 0.689539253836088 1103 38-39 0.6959351413549157 1103 40-41 0.7300104821563451 1103 42-43 0.5426804418678657 1103 44-45 0.65716098315621 1103 46-47 0.6226488955650993 1103 48-49 0.3963536130386558 1103 50-51 0.40286615299697814 1103 52-53 0.7546133328051639 1103 54-55 0.4923428161680903 1103 56-57 0.4120306894305372 1103 58-59 0.3225734385395569 1103 60-61 0.8522171805539713 1103 62-63 0.8353535378656289 1103 64-65 0.8479973081471499 1103 66-67 0.44903452881102623 1103 68-69 0.4442253382191659 1103 70-71 0.6648133214444023 1103 72-73 0.535933431880462 1103 74-75 0.31304152472845104 1103 76-77 0.5288017303396231 1103 78-79 0.41703196199241077 1103 80-81 0.4063562476147382 1103 82-83 0.49724240793183583 1103 84-85 0.531130363082827 1103 86-87 0.724717105804821 1103 88-89 0.8779098651920805 1103 90-91 0.823234212061525 1103 92-93 0.7125361844046623 1103 94-95 0.9496761412527306 1103 96-97 0.9450621350379542 1103 98-99 0.9454543109633597 1103 100-101 0.8664567777343635 1104 1 -0.12982450548281932 1104 2 -0.016855081011463824 1104 3 0.13737423777838842 1104 4 0.11907501327535641 1104 5 -0.18037680260446365 1104 6 -0.18161471703147924 1104 7 0.06933673279675645 1104 8 -0.22505654439542155 1104 9 -0.12787885494373796 1104 10-11 0.039352606358718845 1104 12-13 0.05785251331578678 1104 14-15 -0.029698830245685315 1104 16-17 0.2417159772248283 1104 18-19 -0.07271100973991906 1104 20-21 0.1256879407737017 1104 22-23 0.016101695684852757 1104 24-25 -0.08501471082927026 1104 26-27 0.09409926065446683 1104 28-29 -0.08770752293148831 1104 30-31 -0.004257032558555807 1104 32-33 0.39130078745474606 1104 34-35 0.08885597685461732 1104 36-37 0.25121624033407386 1104 38-39 0.17778503370599452 1104 40-41 0.28446636835447237 1104 42-43 0.5936370429438043 1104 44-45 0.7141231141040194 1104 46-47 0.5020654217596672 1104 48-49 0.6657763144422688 1104 50-51 0.5667428490932451 1104 52-53 0.7269500652850951 1104 54-55 0.6640636967195235 1104 56-57 0.5869893822889907 1104 58-59 0.9937797183377199 1104 60-61 0.8674048568394923 1104 62-63 1.0358503362144518 1104 64-65 0.8832377240558245 1104 66-67 0.5526417410101203 1104 68-69 0.730974786066465 1104 70-71 1.062101792718554 1104 72-73 0.999357835638996 1104 74-75 1.2025776051953194 1104 76-77 0.9374726139508383 1104 78-79 0.8841281116661257 1104 80-81 0.9421335172822225 1104 82-83 1.2977136153242839 1104 84-85 1.0235420881914443 1104 86-87 1.28344556906789 1104 88-89 1.1845006491098324 1104 90-91 1.4284464222856705 1104 92-93 1.3714949662751366 1104 94-95 1.137196655742219 1104 96-97 1.1022267683362372 1104 98-99 0.9492873911687525 1104 100-101 1.607899106288862 1105 1 -0.0038046919630048137 1105 2 0.03290534374896481 1105 3 -0.16152824862410142 1105 4 -0.1495406303402831 1105 5 -0.2749442721719362 1105 6 -0.2694319923487498 1105 7 -0.15414811568809483 1105 8 -0.2080110898499683 1105 9 -0.40962257418745907 1105 10-11 -0.16113787538176894 1105 12-13 -0.09015804094477176 1105 14-15 -0.22450749380435298 1105 16-17 -0.14213859412974017 1105 18-19 -0.39968264921156305 1105 20-21 -0.31726725218149454 1105 22-23 -0.298081805998649 1105 24-25 -0.34234765566222336 1105 26-27 -0.15150582870062124 1105 28-29 -0.24496924269320886 1105 30-31 -0.15542415247567476 1105 32-33 -0.6483667209627626 1105 34-35 -0.4766872761886347 1105 36-37 -0.2934532105853833 1105 38-39 -0.12668595826499285 1105 40-41 -0.2759616295735299 1105 42-43 -0.051605446048689885 1105 44-45 -0.09522841399750348 1105 46-47 -0.19042842698418383 1105 48-49 -0.5933291634132161 1105 50-51 -0.7684439548435549 1105 52-53 -0.5883908438058114 1105 54-55 -0.9419888155829881 1105 56-57 -0.7678895350899282 1105 58-59 -0.7183253062673032 1105 60-61 -0.8700740993894627 1105 62-63 -0.8378708500067376 1105 64-65 -1.0258369975188977 1105 66-67 -0.4622345863662076 1105 68-69 -0.5593159417242681 1105 70-71 -0.6118816312648647 1105 72-73 -0.36857631354515163 1105 74-75 -0.22956432694661189 1105 76-77 -0.4119025479243206 1105 78-79 -0.7119043281163115 1105 80-81 -0.5201414762427703 1105 82-83 -0.36601501715434637 1105 84-85 -0.3738047779054199 1105 86-87 -0.08036885724653686 1105 88-89 -0.5526788380696566 1105 90-91 -0.544180487841242 1105 92-93 -0.30123392520375347 1105 94-95 -0.471203046407485 1105 96-97 -0.39220472609525814 1105 98-99 -0.40214358526222327 1105 100-101 0.27271230235205834 1106 1 0.03550439194769339 1106 2 0.018546007507410422 1106 3 0.0669327470135741 1106 4 0.024257590513364846 1106 5 -0.09601389838012864 1106 6 0.020272695017595765 1106 7 0.19544772726802506 1106 8 0.1902279401731235 1106 9 0.1008716093725468 1106 10-11 0.1594853604406623 1106 12-13 0.11269297693223024 1106 14-15 0.03371196988566538 1106 16-17 0.13332436883322174 1106 18-19 0.07816890326469661 1106 20-21 0.2504557826316116 1106 22-23 0.11624866188262928 1106 24-25 0.16812514392721312 1106 26-27 0.24005338351389582 1106 28-29 0.2592915134439906 1106 30-31 0.1645522182368353 1106 32-33 0.19650059149981303 1106 34-35 -0.1053385891644254 1106 36-37 0.005112459588261231 1106 38-39 0.2735310890363465 1106 40-41 0.2741356674925939 1106 42-43 -0.09379869299078081 1106 44-45 0.14633293661407265 1106 46-47 0.44846313490102574 1106 48-49 0.25066140625576594 1106 50-51 0.16435424462611792 1106 52-53 0.14926793217571088 1106 54-55 0.25778291053111246 1106 56-57 0.21614198488269665 1106 58-59 -0.4415686130845202 1106 60-61 0.11000405902299093 1106 62-63 0.22384863506725594 1106 64-65 0.17586195894333656 1106 66-67 0.15255991367656208 1106 68-69 0.21817849844338255 1106 70-71 -0.09659209230327193 1106 72-73 0.2015978588951839 1106 74-75 0.2512756354175991 1106 76-77 0.27487039707610705 1106 78-79 0.2956415405005721 1106 80-81 0.48230966138446263 1106 82-83 0.43018237827803674 1106 84-85 0.3009744325983874 1106 86-87 0.23250536223853757 1106 88-89 0.4174905229773067 1106 90-91 0.31974796139321526 1106 92-93 0.3341773954702667 1106 94-95 0.18044939991874998 1106 96-97 0.5873094300932635 1106 98-99 0.6819488790265495 1106 100-101 1.756318815909495 1107 1 0.0693013686430568 1107 2 0.1357672966109149 1107 3 0.02571922612337829 1107 4 0.034634453834797796 1107 5 0.04054394331627975 1107 6 -0.17957508999185023 1107 7 0.075397338857357 1107 8 0.04767072833185182 1107 9 -0.05427745634234071 1107 10-11 -0.1551404006342949 1107 12-13 0.023878154341424818 1107 14-15 0.061793011246159324 1107 16-17 0.020824368833224582 1107 18-19 -0.00814224517115747 1107 20-21 0.03521591280167513 1107 22-23 0.10814715023030885 1107 24-25 0.15373820292364115 1107 26-27 0.07545124200645148 1107 28-29 0.11357452835056137 1107 30-31 0.019344366042844285 1107 32-33 -0.08786587921191824 1107 34-35 0.16956693256557287 1107 36-37 -0.07929075267291807 1107 38-39 -0.20088629496532917 1107 40-41 -0.0315592726711742 1107 42-43 0.22871862802538345 1107 44-45 0.3485636735445752 1107 46-47 0.3848451420393886 1107 48-49 0.6534511396170899 1107 50-51 0.2229491427995427 1107 52-53 0.4530864289214662 1107 54-55 0.3080322281880541 1107 56-57 0.4200313403309508 1107 58-59 0.45368647824447805 1107 60-61 0.5764957659304102 1107 62-63 0.5525752779393969 1107 64-65 0.6041293324474353 1107 66-67 0.2960566594250409 1107 68-69 0.5446402872319673 1107 70-71 0.3766705572873228 1107 72-73 0.35547671675787385 1107 74-75 0.35368483130254447 1107 76-77 0.0630553645335894 1107 78-79 -0.01613412609610876 1107 80-81 0.09233747998618469 1107 82-83 0.06472993234060453 1107 84-85 0.08968427933363543 1107 86-87 0.2623499979723185 1107 88-89 0.08633631344549642 1107 90-91 0.08342893976818644 1107 92-93 0.45232247210264376 1107 94-95 0.5329425764881393 1107 96-97 0.7135612671707356 1107 98-99 0.5610298154111781 1107 100-101 0.9209235818133372 1108 1 0.09663063142008 1108 2 0.0839694675742777 1108 3 0.11531669333278671 1108 4 0.10508899928934312 1108 5 0.08499215679434968 1108 6 -0.0014317159529539936 1108 7 -0.027904334593564784 1108 8 0.015558561891324985 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-1.3158620460116524 2316 52-53 -1.3157946899596595 2316 54-55 -1.1283314081755833 2316 56-57 -1.2216644638648617 2316 58-59 -1.0451771581191593 2316 60-61 -1.2721930452584047 2316 62-63 -1.5046799668158535 2316 64-65 -1.2296119262938277 2316 66-67 -1.412946837078458 2316 68-69 -1.1436464260547474 2316 70-71 -1.4011266455098799 2316 72-73 -1.287967339186178 2316 74-75 -1.0720144693967555 2316 76-77 -1.2286404396622146 2316 78-79 -1.2849278323898154 2316 80-81 -1.115529652880948 2316 82-83 -1.0183120257013556 2316 84-85 -1.310752783603423 2316 86-87 -1.408716435594112 2316 88-89 -1.5116531970440121 2316 90-91 -2.104508123168877 2316 92-93 -1.932555150274979 2316 94-95 -2.054732248686687 2316 96-97 -1.8318058656963991 2316 98-99 -2.291068086686721 2316 100-101 -1.5756673272775714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 34.0 6 104.0 7 183.0 8 325.0 9 610.0 10 957.0 11 1417.0 12 1977.0 13 2628.0 14 2985.0 15 3896.0 16 4948.0 17 6263.0 18 5889.0 19 3934.0 20 3434.0 21 3600.0 22 3782.0 23 4020.0 24 4623.0 25 5259.0 26 5727.0 27 6393.0 28 7712.0 29 9363.0 30 11026.0 31 13156.0 32 15983.0 33 19865.0 34 26432.0 35 37544.0 36 49288.0 37 81075.0 38 81598.0 39 8835.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.010141403431945 23.677411176287556 15.729086236502138 20.583361183778365 2 15.29562449985743 24.84754274781777 38.7802500022995 21.076582750025295 3 17.446122572871346 29.2504070125737 33.17704357104094 20.126426843514015 4 11.62239350987408 21.582014183353415 39.17622494688141 27.619367359891093 5 10.900722482524479 39.97644628764511 35.85132639142866 13.271504838401752 6 25.017016814452337 40.84541373854814 19.30166672798852 14.835902719011 7 22.62116097462265 37.0305558366063 23.64214166796972 16.70614152080133 8 23.2719211913281 35.89620949420065 22.462265105455348 18.369604209015904 9 24.274045934933174 18.45675548892098 23.798048179251097 33.47115039689474 10-11 21.68399727738482 29.347561143866297 29.436437053320947 19.532004525427936 12-13 23.405522493768338 28.265528564464354 29.53681049310608 18.792138448661227 14-15 20.88204453683349 29.59084888565936 28.813845785925185 20.71326079158197 16-17 18.03257577673888 32.54588226636895 29.911374386875867 19.510167570016304 18-19 17.683316623589253 30.588604567738848 33.44436115122472 18.28371765744718 20-21 19.026228166189902 29.511975827592234 33.20141833534157 18.260377670876295 22-23 18.6089827904966 30.112261886146857 32.926052483926455 18.35270283943009 24-25 18.58331378744188 30.517690181523843 33.20858455552643 17.690411475507847 26-27 18.189636637282305 30.329628381913153 33.75940083036596 17.72133415043858 28-29 17.74057432463507 30.286909371866923 34.00992466818128 17.962591635316734 30-31 18.476761191696024 30.204817005307262 33.416997029038164 17.901424773958556 32-33 17.870611392672853 30.423155106283172 33.623262723167066 18.082970777876913 34-35 18.119194058579428 30.385044803269206 33.59480551138278 17.90095562676859 36-37 18.593039368448892 29.877128121388445 33.21485042059326 18.3149820895694 38-39 17.906182765714497 30.451791400221012 33.331646786495824 18.31037904756867 40-41 17.88880954762833 30.013074059892304 33.799951935294324 18.298164457185038 42-43 17.90777389506558 30.137052458009787 33.18063120711721 18.77454243980742 44-45 18.045419685389742 30.00077037084733 33.02740795505634 18.926401988706594 46-47 18.533111966022265 30.158345080057856 32.229179553570816 19.079363400349067 48-49 18.243654985754333 30.038586332895655 32.32283745219825 19.39492122915177 50-51 17.73105176079131 30.396400808967165 32.28728682117066 19.585260609070872 52-53 17.982942264001693 30.766011460738234 32.0476411666774 19.20340510858268 54-55 18.001453287833773 30.25207185496555 31.610849989422274 20.1356248677784 56-57 18.390501956338525 30.58027886591004 31.044902079594177 19.98431709815726 58-59 18.13695675419 30.227341451384248 31.629240160092166 20.006461634333583 60-61 18.22528014570106 30.76808098182646 31.23834403018882 19.768294842283662 62-63 17.830501069285074 30.68204290937522 31.44158024237128 20.045875778968426 64-65 17.7269680374642 30.689852219693996 31.211619144637975 20.371560598203825 66-67 17.80327812304172 30.618428087684908 30.53173202407713 21.046561765196245 68-69 17.84261896248228 30.164104816447047 30.926968585189517 21.066307635881156 70-71 18.19992158675299 30.132072282756788 30.712698232022728 20.955307898467492 72-73 18.250719745398687 29.581075984878446 30.85741682686559 21.310787442857272 74-75 17.839806104310913 30.281944383762614 30.27895500525441 21.599294506672063 76-77 18.022201028715923 29.63201144909513 30.802805672548057 21.542981849640892 78-79 17.833294188622244 29.599618684461394 30.706838350764244 21.860248776152122 80-81 17.977933736823708 29.398995566511527 30.44562537942199 22.177445317242775 82-83 17.994511022221506 29.294860665736888 30.51890041081101 22.191727901230593 84-85 17.775526816839744 29.10979221663187 30.307145025248577 22.807535941279813 86-87 17.745215730913856 29.202370525196326 30.372900785763036 22.679512958126782 88-89 18.126574158362494 28.60050326917286 30.285681980494573 22.987240591970075 90-91 17.904003213281563 28.3006541982724 30.535523843380226 23.259818745065814 92-93 18.064950071572703 28.579871111647666 30.428228963661763 22.92694985311787 94-95 17.833921818643812 27.865344749680084 30.54646926060107 23.754264171075036 96-97 18.028723163296164 27.71114951290657 30.438021916617515 23.822105407179755 98-99 17.929478747964936 27.82505817750347 30.114446416909647 24.13101665762194 100-101 17.848092533400788 27.282406236346496 30.943615333318004 23.925885896934716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3682.0 1 2275.5 2 954.0 3 989.5 4 782.0 5 758.5 6 1207.5 7 1953.5 8 2540.0 9 2645.5 10 2085.0 11 1431.5 12 1225.5 13 1148.0 14 1136.0 15 1101.0 16 1101.0 17 1130.0 18 1123.0 19 1196.0 20 1364.0 21 1565.0 22 1801.0 23 2118.0 24 2427.5 25 2910.0 26 3965.5 27 5127.5 28 5786.5 29 6571.0 30 7724.0 31 9008.5 32 10684.5 33 12609.5 34 14678.5 35 16198.5 36 17517.0 37 18918.5 38 18831.0 39 17526.0 40 16625.0 41 16218.0 42 15560.5 43 15333.0 44 15417.0 45 15021.5 46 14240.0 47 14121.5 48 15247.0 49 14751.0 50 12922.5 51 11081.5 52 8813.0 53 7094.0 54 6049.5 55 5357.5 56 4628.5 57 3917.5 58 3363.0 59 2825.5 60 2343.0 61 1930.5 62 1525.0 63 1144.0 64 835.5 65 624.5 66 447.5 67 364.0 68 282.0 69 202.5 70 154.0 71 107.0 72 92.0 73 77.5 74 56.5 75 42.0 76 28.5 77 20.0 78 17.5 79 12.0 80 6.5 81 5.5 82 4.5 83 3.5 84 3.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23248006328240695 2 0.0 3 0.0 4 0.0 5 0.028743825826212526 6 0.0027594072793164027 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002989357885926103 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.5990121321940044E-4 26-27 0.0017246295495727518 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007013493501595857 36-37 0.016901369585812967 38-39 0.014371912913106263 40-41 0.010462752600741362 42-43 0.00977290078091226 44-45 0.004713987435498855 46-47 6.898518198291007E-4 48-49 0.0016096542462679018 50-51 1.1497530330485011E-4 52-53 0.0 54-55 0.0 56-57 0.0017246295495727518 58-59 3.4492590991455033E-4 60-61 0.002989357885926103 62-63 0.0013797036396582013 64-65 0.0017246295495727518 66-67 0.0054038392553279555 68-69 3.4492590991455033E-4 70-71 1.1497530330485011E-4 72-73 0.0 74-75 6.898518198291007E-4 76-77 0.05898233059538811 78-79 0.13567085789972314 80-81 9.198024264388009E-4 82-83 0.001034777729743651 84-85 0.0 86-87 0.10520240252393787 88-89 0.3471104406773425 90-91 0.38493731546463816 92-93 8.048271231339509E-4 94-95 5.748765165242506E-4 96-97 1.1497530330485011E-4 98-99 0.0 100-101 0.0013797036396582013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 434876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64890671909817 #Duplication Level Percentage of deduplicated Percentage of total 1 86.64944799902945 49.95245944957396 2 7.657369237122779 8.82877929729166 3 1.8658056496612923 3.226849675598706 4 0.8786917595436483 2.0262247712308805 5 0.5249084196582061 1.5130198260472576 6 0.33710423322644895 1.16602142975308 7 0.26795943933119887 1.081329810575429 8 0.1982103123814551 0.914128624739345 9 0.1699324765914755 0.8816779342410574 >10 1.2652536840571957 14.376024703990831 >50 0.12341139638814803 4.938958822460254 >100 0.0567133277886316 5.453783862077867 >500 0.001996948161571535 0.8171350920975367 >1k 0.0031951170585144562 4.8236067003221725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4544 1.044895556434478 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4129 0.9494660546914523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.8669137869185699 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2550 0.5863740468547356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2132 0.49025469329188087 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1522 0.3499848232599638 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1188 0.27318132065232387 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1115 0.25639492636981576 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 772 0.17752186830268857 TruSeq Adapter, Index 1 (95% over 21bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 728 0.1674040416118618 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.16510453554576476 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.16349488129949685 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.14256937609801415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 480 0.11037629117265611 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2995060660970025E-4 0.0 12-13 0.0 0.0 0.0 2.2995060660970025E-4 0.0 14-15 0.0 0.0 0.0 3.449259099145504E-4 0.0 16-17 0.0 0.0 0.0 8.048271231339509E-4 0.0 18-19 0.0 0.0 0.0 0.001149753033048501 2.2995060660970025E-4 20-21 0.0 0.0 0.0 0.002299506066097002 4.599012132194005E-4 22-23 0.0 0.0 0.0 0.006208666378461907 4.599012132194005E-4 24-25 0.0 0.0 0.0 0.011267579723875312 4.599012132194005E-4 26-27 0.0 0.0 0.0 0.02540954203037188 4.599012132194005E-4 28-29 0.0 0.0 0.0 0.05863740468547356 4.599012132194005E-4 30-31 0.0 0.0 0.0 0.131186821070834 4.599012132194005E-4 32-33 0.0 0.0 0.0 0.25329059318058483 4.599012132194005E-4 34-35 0.0 0.0 0.0 0.44587422621620876 4.599012132194005E-4 36-37 0.0 0.0 0.0 0.7295182994692739 4.599012132194005E-4 38-39 0.0 0.0 0.0 1.0999687267175011 4.599012132194005E-4 40-41 0.0 0.0 0.0 1.4867456470350169 4.599012132194005E-4 42-43 0.0 0.0 0.0 1.8659341973344126 4.599012132194005E-4 44-45 0.0 0.0 0.0 2.2808800669616165 4.599012132194005E-4 46-47 0.0 0.0 0.0 2.7572227485536107 4.599012132194005E-4 48-49 0.0 0.0 0.0 3.230231146349764 4.599012132194005E-4 50-51 0.0 0.0 0.0 3.719681012518511 4.599012132194005E-4 52-53 0.0 0.0 0.0 4.180042126951131 4.599012132194005E-4 54-55 0.0 0.0 0.0 4.692027152567628 4.599012132194005E-4 56-57 0.0 0.0 0.0 5.31220393859399 4.599012132194005E-4 58-59 0.0 0.0 0.0 5.952386427395395 4.599012132194005E-4 60-61 0.0 0.0 0.0 6.602916693494237 4.599012132194005E-4 62-63 0.0 0.0 0.0 7.257471095208749 4.599012132194005E-4 64-65 0.0 0.0 0.0 7.915359780719101 4.599012132194005E-4 66-67 0.0 0.0 0.0 8.667643190242735 4.599012132194005E-4 68-69 0.0 0.0 0.0 9.495810299947571 4.599012132194005E-4 70-71 0.0 0.0 0.0 10.400091060440218 4.599012132194005E-4 72-73 0.0 0.0 0.0 11.262865736439814 4.599012132194005E-4 74-75 0.0 0.0 0.0 12.156798719635022 4.599012132194005E-4 76-77 0.0 0.0 0.0 13.095802021725733 4.599012132194005E-4 78-79 0.0 0.0 0.0 14.114483209006705 4.599012132194005E-4 80-81 0.0 0.0 0.0 15.168576789705572 4.599012132194005E-4 82-83 0.0 0.0 0.0 16.205769000818623 4.599012132194005E-4 84-85 0.0 0.0 0.0 17.24399598966142 4.599012132194005E-4 86-87 0.0 0.0 0.0 18.329822754072424 4.599012132194005E-4 88-89 0.0 0.0 0.0 19.41771907394292 4.599012132194005E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTATA 55 0.0025627043 34.58703 1 CATTCCG 115 1.0438998E-7 33.007164 9 GTATGGT 70 0.008336861 27.175522 1 GGTATCA 3885 0.0 26.318638 1 CCTTTAG 165 8.2878614E-8 25.940271 1 TTAGGCA 150 1.0645526E-6 25.314224 4 GTGCAGG 180 1.9227446E-7 23.778582 1 CTATGGG 100 0.001659269 23.732084 3 CCCTATA 100 0.001659269 23.732084 2 TCTAGTC 105 0.0022036834 22.601986 3 GCATTCC 210 3.473906E-8 22.594189 8 GAGTACT 2780 0.0 22.443832 12-13 GTACATG 2570 0.0 22.390726 1 GTACCCA 170 3.1088766E-6 22.37984 1 TACTGTA 150 3.0534335E-5 22.142305 7 TATGCTA 110 0.0028871335 21.574623 5 ACTATAC 135 3.8100724E-4 21.095186 3 TAGGGTA 115 0.003735906 20.636597 5 AGGGTAG 115 0.0037433826 20.629477 6 GTATCAA 4995 0.0 20.56526 1 >>END_MODULE