##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938805_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1450034 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702551112594602 33.0 31.0 34.0 30.0 34.0 2 31.726374691903775 33.0 31.0 34.0 30.0 34.0 3 31.72721536184669 33.0 31.0 34.0 30.0 34.0 4 35.24665145782789 37.0 35.0 37.0 33.0 37.0 5 35.30963549820211 37.0 35.0 37.0 33.0 37.0 6 35.44975359198474 37.0 35.0 37.0 33.0 37.0 7 35.415709562672326 37.0 35.0 37.0 33.0 37.0 8 35.41037382571719 37.0 35.0 37.0 33.0 37.0 9 36.934917388144 39.0 37.0 39.0 33.0 39.0 10-11 36.841039589416525 39.0 37.0 39.0 32.5 39.0 12-13 36.79587444156482 39.0 37.0 39.0 32.5 39.0 14-15 37.79279692752032 40.0 37.0 41.0 32.0 41.0 16-17 37.781080650522675 40.0 37.5 41.0 32.5 41.0 18-19 37.88023211869515 40.0 37.5 41.0 33.0 41.0 20-21 37.7820116631748 40.0 37.0 41.0 32.5 41.0 22-23 37.71095643274572 40.0 37.0 41.0 32.0 41.0 24-25 37.684454985193454 40.0 37.0 41.0 32.0 41.0 26-27 37.50830152948138 40.0 37.0 41.0 32.0 41.0 28-29 37.42520175388991 40.0 37.0 41.0 32.0 41.0 30-31 37.30016089277906 40.0 36.5 41.0 32.0 41.0 32-33 37.06629706613776 39.0 36.0 41.0 31.0 41.0 34-35 36.71838246551461 39.0 35.0 41.0 30.5 41.0 36-37 36.38766573749305 39.0 35.0 41.0 30.0 41.0 38-39 36.16046106505088 39.0 35.0 40.5 30.0 41.0 40-41 36.08699382221383 39.0 35.0 41.0 30.0 41.0 42-43 35.888855364770755 39.0 35.0 41.0 30.0 41.0 44-45 35.810304448033634 39.0 35.0 41.0 29.0 41.0 46-47 35.54309243783249 39.0 35.0 41.0 27.0 41.0 48-49 35.25634433399493 39.0 35.0 41.0 25.0 41.0 50-51 34.06567018428534 38.0 34.0 40.0 23.5 40.5 52-53 34.215886317148424 38.0 33.5 39.5 23.0 40.5 54-55 34.84575465127024 39.0 35.0 40.5 24.0 41.0 56-57 34.79005733658659 39.0 35.0 41.0 23.0 41.0 58-59 34.64953339025154 39.0 35.0 41.0 23.0 41.0 60-61 34.29149454426586 38.5 34.0 40.0 22.0 41.0 62-63 33.96425807946572 38.0 34.0 40.0 21.5 41.0 64-65 33.52683212945352 37.5 33.5 40.0 19.5 41.0 66-67 33.02360806712119 37.0 33.0 39.0 16.5 41.0 68-69 32.5962170542208 36.0 32.5 39.0 13.0 40.5 70-71 32.03329749509322 36.0 32.0 38.5 9.5 40.0 72-73 31.50402645731066 35.0 31.5 37.0 6.5 39.5 74-75 31.032835092142665 35.0 31.0 37.0 2.0 39.0 76-77 30.541805571455566 35.0 31.0 36.5 2.0 39.0 78-79 30.058649314429868 34.5 30.5 36.0 2.0 38.0 80-81 29.558000708948896 34.0 30.0 36.0 2.0 37.0 82-83 29.041742124667422 34.0 29.0 35.0 2.0 37.0 84-85 28.583022191203792 34.0 29.0 35.0 2.0 36.0 86-87 28.249440357950228 34.0 29.0 35.0 2.0 36.0 88-89 27.744184619119274 34.0 28.0 35.0 2.0 35.5 90-91 27.211114704896573 34.0 26.0 35.0 2.0 35.0 92-93 26.775494229790475 34.0 25.0 35.0 2.0 35.0 94-95 26.24475357129557 33.5 24.5 35.0 2.0 35.0 96-97 25.58182463307757 33.0 20.5 35.0 2.0 35.0 98-99 24.92945027495907 33.0 10.0 35.0 2.0 35.0 100-101 22.8153919149482 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12498954517740657 1101 2 0.026437357512207882 1101 3 0.2160023997645304 1101 4 0.24157637695887502 1101 5 0.1396251183282331 1101 6 0.11177443688919908 1101 7 0.1845229555895287 1101 8 0.23446599605964735 1101 9 0.08785926189511173 1101 10-11 0.2850540626172844 1101 12-13 0.27924738453673115 1101 14-15 0.3790942335104859 1101 16-17 0.37741577759491207 1101 18-19 0.46226021841468423 1101 20-21 0.4980621792270128 1101 22-23 0.506278547652137 1101 24-25 0.5130443888056249 1101 26-27 0.4206354711136271 1101 28-29 0.3511690295629819 1101 30-31 0.476935285290331 1101 32-33 0.4529435012440004 1101 34-35 0.38858111528934103 1101 36-37 0.44900276611453904 1101 38-39 0.48216326176483193 1101 40-41 0.534423959570546 1101 42-43 0.4356655604981299 1101 44-45 0.6968147649615588 1101 46-47 0.5211092406566706 1101 48-49 0.5822241385746167 1101 50-51 0.5966221343791105 1101 52-53 0.533762527591719 1101 54-55 0.5986719507372271 1101 56-57 0.5782086037936907 1101 58-59 0.5139851400726982 1101 60-61 0.6665629763384757 1101 62-63 0.602785127794391 1101 64-65 0.552647092992558 1101 66-67 0.4406399478273286 1101 68-69 0.48430013041248543 1101 70-71 0.4430123918367954 1101 72-73 0.4648568175527892 1101 74-75 0.39579770753922006 1101 76-77 0.3335607075967211 1101 78-79 0.19573838433337087 1101 80-81 0.25097416101519343 1101 82-83 0.2658555629828214 1101 84-85 0.45898135619895797 1101 86-87 0.4274615224044993 1101 88-89 0.2833372444503901 1101 90-91 0.1739277996939741 1101 92-93 -0.11067651242515453 1101 94-95 -0.3361640223618352 1101 96-97 -0.339661044093166 1101 98-99 -0.21781604153443013 1101 100-101 0.04048174584498199 1102 1 0.3270297508602802 1102 2 0.34135379884382644 1102 3 0.3033156671965962 1102 4 0.1990866832667777 1102 5 0.10602821686983788 1102 6 0.100416442297238 1102 7 0.1676926439622406 1102 8 0.1237817901994589 1102 9 0.3334918253952637 1102 10-11 0.27510321151572725 1102 12-13 0.2795280374912039 1102 14-15 0.17404746725311782 1102 16-17 0.24996802946778018 1102 18-19 0.33850500985047915 1102 20-21 0.33728765464702093 1102 22-23 0.47275506478499096 1102 24-25 0.28437151988413234 1102 26-27 0.3096204660158861 1102 28-29 0.2843162767813894 1102 30-31 0.29275740728155597 1102 32-33 0.1390355265997414 1102 34-35 0.21796656012288906 1102 36-37 0.29595464784689085 1102 38-39 0.31914517585676805 1102 40-41 0.47748548712052497 1102 42-43 0.45743264902155545 1102 44-45 0.4483681915318911 1102 46-47 0.4194895988285765 1102 48-49 0.4260409079349614 1102 50-51 0.4364557373757023 1102 52-53 0.4362266217448294 1102 54-55 0.2592331458256609 1102 56-57 0.1923889198538049 1102 58-59 0.32669708430781697 1102 60-61 0.30843096694089667 1102 62-63 0.24099742665515578 1102 64-65 0.41455725080097494 1102 66-67 0.3782169680853187 1102 68-69 0.23687282867039272 1102 70-71 0.273944336958472 1102 72-73 0.41324188559658737 1102 74-75 0.2862807187542593 1102 76-77 0.2532576688981969 1102 78-79 0.24240628849578982 1102 80-81 0.1740087591800048 1102 82-83 0.0025845292490558336 1102 84-85 -0.3681469517934133 1102 86-87 -0.3035014524614077 1102 88-89 -0.23913438549774568 1102 90-91 -0.1805008651404627 1102 92-93 -0.367954649753937 1102 94-95 -0.30115720690015735 1102 96-97 -0.4866223455692982 1102 98-99 -0.35934159674521027 1102 100-101 -0.16836976574684215 1103 1 -0.013781836932729163 1103 2 0.13896547509327561 1103 3 0.22808346905418375 1103 4 0.18554179162590856 1103 5 0.08140968524666192 1103 6 0.2134527094356713 1103 7 0.14850865457701445 1103 8 0.11036958321140133 1103 9 0.3117868276066744 1103 10-11 0.14219465335562376 1103 12-13 0.2128346496097322 1103 14-15 0.35719651634644123 1103 16-17 0.27359971898569313 1103 18-19 0.34033218809020127 1103 20-21 0.32361560688055135 1103 22-23 0.3338867543029025 1103 24-25 0.24595654863247773 1103 26-27 0.23574275703755632 1103 28-29 0.2880507969052246 1103 30-31 0.2228353595150807 1103 32-33 0.24792539833790528 1103 34-35 0.41380081487741904 1103 36-37 0.33009330330907005 1103 38-39 0.29452111570638806 1103 40-41 0.4119060974699309 1103 42-43 0.3603821182829492 1103 44-45 0.5207117342121208 1103 46-47 0.33886708666581455 1103 48-49 0.3614511246532288 1103 50-51 0.29314801955172953 1103 52-53 0.22032104898941185 1103 54-55 0.399790421367463 1103 56-57 0.405466650946245 1103 58-59 0.209732798593528 1103 60-61 0.1499833773787529 1103 62-63 0.2100625527055726 1103 64-65 0.14145077136709716 1103 66-67 0.28757123610831314 1103 68-69 0.18963156374336876 1103 70-71 -0.037126538760240635 1103 72-73 0.039621253133077516 1103 74-75 0.08759927249154131 1103 76-77 -0.1256814067320562 1103 78-79 -0.22678599368024166 1103 80-81 -0.29797819349580124 1103 82-83 -0.06805069852626389 1103 84-85 -0.23034841132459505 1103 86-87 -0.456141324199578 1103 88-89 -0.6360107676295463 1103 90-91 -0.5962378178162702 1103 92-93 -0.39200927160267796 1103 94-95 -0.524092301990823 1103 96-97 -0.4152158882495236 1103 98-99 -0.3417719373024859 1103 100-101 -0.18528982324352583 1104 1 0.02362229031508889 1104 2 0.08654748607481721 1104 3 -0.02179193682061964 1104 4 0.1339145168392264 1104 5 0.1282484464250686 1104 6 0.06336479236897219 1104 7 0.06442865544000398 1104 8 0.07252640999860205 1104 9 0.058491825395265096 1104 10-11 0.202389796881576 1104 12-13 0.10468047651559687 1104 14-15 0.09901159924738323 1104 16-17 0.07838445701440833 1104 18-19 -0.04814312501322604 1104 20-21 0.07579195880772005 1104 22-23 0.19139746076777442 1104 24-25 0.1384156375311889 1104 26-27 0.2958650858150236 1104 28-29 0.1562943972978914 1104 30-31 0.08864155362302029 1104 32-33 0.049508984275490775 1104 34-35 0.1466699317154223 1104 36-37 0.38933700078806766 1104 38-39 0.2737004125856046 1104 40-41 0.1936619577087626 1104 42-43 0.023062132522269962 1104 44-45 0.23996611118754885 1104 46-47 0.2832808470351722 1104 48-49 0.2492295736451524 1104 50-51 0.0970816340758418 1104 52-53 0.021502805389019386 1104 54-55 0.07013701957028218 1104 56-57 0.1941733531393126 1104 58-59 0.23160203409261015 1104 60-61 0.3831081548892499 1104 62-63 0.32827325161927945 1104 64-65 0.25118745166180645 1104 66-67 0.1875426495774235 1104 68-69 0.23742878275934487 1104 70-71 0.2329023713917593 1104 72-73 0.3512171366726271 1104 74-75 0.21907304300102837 1104 76-77 0.037395043360174185 1104 78-79 0.0731541364154431 1104 80-81 0.03632941915131482 1104 82-83 0.1812340988329879 1104 84-85 0.112573995121938 1104 86-87 0.11042430076671295 1104 88-89 0.23728240072894025 1104 90-91 -0.00934770875595703 1104 92-93 -0.05760673009827144 1104 94-95 0.09800886196641301 1104 96-97 0.27148382372768864 1104 98-99 0.2160170833121775 1104 100-101 0.6061191151713778 1105 1 -0.2151716751646724 1105 2 -0.04497164042279067 1105 3 -0.04061099437712201 1105 4 -8.56021087692227E-4 1105 5 -0.0725357844882808 1105 6 -0.048953305601919794 1105 7 -0.05165842980059665 1105 8 -2.2508527800368938E-4 1105 9 0.08319261480193774 1105 10-11 0.003746259644074712 1105 12-13 -0.010773613472210286 1105 14-15 0.27572389540013376 1105 16-17 0.07271493125880113 1105 18-19 0.21197341236299394 1105 20-21 0.10380471601703078 1105 22-23 0.22968306325710586 1105 24-25 0.2996286075676835 1105 26-27 0.2909983201878532 1105 28-29 0.2507871833258264 1105 30-31 0.1917690573961508 1105 32-33 0.2069669415899753 1105 34-35 0.37992065994463076 1105 36-37 0.387236160451927 1105 38-39 0.36196624367784125 1105 40-41 0.2466710401296055 1105 42-43 0.10437847280561385 1105 44-45 0.20203246026968458 1105 46-47 0.08278749374411376 1105 48-49 0.15207541264594227 1105 50-51 0.38579630693686084 1105 52-53 0.27348046267513837 1105 54-55 0.40332957227502675 1105 56-57 0.03165712713671809 1105 58-59 0.13888142774215595 1105 60-61 0.2838065717282703 1105 62-63 0.21547380811682615 1105 64-65 0.24154190830033428 1105 66-67 0.1555745777958677 1105 68-69 0.29920094173379397 1105 70-71 0.29029428602900254 1105 72-73 0.28177967950203 1105 74-75 0.18864734722382792 1105 76-77 0.33290445185380335 1105 78-79 0.4473045356734495 1105 80-81 0.44414605652318784 1105 82-83 0.26943316264180694 1105 84-85 0.2608796588508433 1105 86-87 0.15537990311112182 1105 88-89 0.1429479929081623 1105 90-91 0.3734477597640442 1105 92-93 0.3274712478778419 1105 94-95 0.512385255539364 1105 96-97 0.26320364543316543 1105 98-99 0.2865564594469667 1105 100-101 0.18290718442716525 1106 1 0.12935079976177022 1106 2 0.2252659179083203 1106 3 0.27191949426959283 1106 4 0.22405124740568283 1106 5 0.18431489298606607 1106 6 0.16953408935606262 1106 7 0.2198365135582705 1106 8 0.23563508573454328 1106 9 0.24098651001609994 1106 10-11 0.271882977639045 1106 12-13 0.1674754152374831 1106 14-15 0.19095037299372564 1106 16-17 0.24728819247273748 1106 18-19 0.29032552721405125 1106 20-21 0.4850286185024473 1106 22-23 0.45002738937747466 1106 24-25 0.25694859288200433 1106 26-27 0.13012897062255036 1106 28-29 0.40040858719952865 1106 30-31 0.3609315745388173 1106 32-33 0.3144784748633782 1106 34-35 0.33909962178128694 1106 36-37 0.36276010496949596 1106 38-39 0.3848609802508207 1106 40-41 0.5446541618193166 1106 42-43 0.5072067454070961 1106 44-45 0.496547674168319 1106 46-47 0.44052964135160266 1106 48-49 0.42447287108166165 1106 50-51 0.49784393723395937 1106 52-53 0.2984254169255536 1106 54-55 0.24300352144579307 1106 56-57 0.18776896946400967 1106 58-59 0.10763702052326352 1106 60-61 0.10921055588490702 1106 62-63 0.0755075258755653 1106 64-65 0.1767073216726942 1106 66-67 0.17821696808531584 1106 68-69 0.27242385631036115 1106 70-71 0.1589062434077917 1106 72-73 -0.0108855063240334 1106 74-75 0.01606013051896582 1106 76-77 -0.05565002068366098 1106 78-79 0.06865983208189874 1106 80-81 -0.1880660104868923 1106 82-83 -0.18607156571905392 1106 84-85 -0.14990634229660316 1106 86-87 -0.1302068032764332 1106 88-89 -0.11710213886415133 1106 90-91 -0.0719741110653409 1106 92-93 -0.010982644084201354 1106 94-95 -0.07319388301638696 1106 96-97 0.13471004280774324 1106 98-99 0.10031467540220618 1106 100-101 0.4056601775557773 1107 1 -0.055007622293747005 1107 2 -0.12793916378301873 1107 3 0.02612731640045851 1107 4 0.04497192375848158 1107 5 0.009137490032117057 1107 6 0.0021386599360440073 1107 7 -0.09785269058111368 1107 8 -0.08221812729909317 1107 9 -0.06348655922646884 1107 10-11 -0.09965804993134952 1107 12-13 -0.0726845481040499 1107 14-15 -0.15042246097062417 1107 16-17 -0.07664229558790225 1107 18-19 -0.04329465601867355 1107 20-21 -0.17547222490086511 1107 22-23 -0.043353857746154745 1107 24-25 -0.08161571301744175 1107 26-27 0.007633212488954655 1107 28-29 -0.2990828858289589 1107 30-31 -0.12015149049915408 1107 32-33 -0.07843415411967669 1107 34-35 -0.12516880412098885 1107 36-37 -0.025356506348309438 1107 38-39 0.1023499444402205 1107 40-41 -0.09272117899613619 1107 42-43 -0.006964368551258815 1107 44-45 0.11675724771536977 1107 46-47 0.1693503776422034 1107 48-49 0.08206454459860169 1107 50-51 0.22707171378029045 1107 52-53 0.06605811254591742 1107 54-55 0.06086667441240934 1107 56-57 0.13070207470901352 1107 58-59 0.13065045036672274 1107 60-61 -0.024656237085174837 1107 62-63 0.012607132950009259 1107 64-65 -0.008998458298933087 1107 66-67 0.019752319953973085 1107 68-69 0.09439592907464345 1107 70-71 0.10199018713584707 1107 72-73 -0.046776367847847666 1107 74-75 -0.0721751635986827 1107 76-77 0.09223083530360654 1107 78-79 0.08452172864098983 1107 80-81 -0.012713664712411088 1107 82-83 0.04450505973333918 1107 84-85 -0.0471080523626739 1107 86-87 -0.13938906486962566 1107 88-89 -0.07794203338158212 1107 90-91 -0.17569979674676617 1107 92-93 -0.05366024335182473 1107 94-95 -0.04070272707176059 1107 96-97 -0.08201876500663374 1107 98-99 -0.12379378715854017 1107 100-101 0.2777789637309205 1108 1 -0.08188028374179268 1108 2 -0.1721410108447543 1108 3 -0.11433139162692996 1108 4 -0.10376798801350162 1108 5 -0.060451021884489364 1108 6 0.022129245065400482 1108 7 -0.033950954653676035 1108 8 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-1.9533488997985664 2316 76-77 -1.6999571600316337 2316 78-79 -1.7291550045281028 2316 80-81 -1.7062237492795091 2316 82-83 -1.6259823521810297 2316 84-85 -1.7249397706060066 2316 86-87 -1.731663729865108 2316 88-89 -2.07638487256067 2316 90-91 -2.140073146987543 2316 92-93 -2.312205170996716 2316 94-95 -2.3124718068249592 2316 96-97 -2.3955877213767955 2316 98-99 -2.498247670505336 2316 100-101 -2.9773020298203967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6255.0 3 1244.0 4 1064.0 5 1114.0 6 1298.0 7 1624.0 8 1928.0 9 2523.0 10 3209.0 11 4624.0 12 6516.0 13 9097.0 14 10002.0 15 10111.0 16 13171.0 17 17033.0 18 14027.0 19 9732.0 20 9494.0 21 10001.0 22 10744.0 23 12237.0 24 13738.0 25 15995.0 26 19051.0 27 22044.0 28 25957.0 29 31029.0 30 36732.0 31 44157.0 32 54116.0 33 67887.0 34 88892.0 35 129640.0 36 175960.0 37 272978.0 38 255471.0 39 39334.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.93900319668893 20.87069020299113 15.064500175444898 18.125806424875044 2 13.484419576326232 25.400132117630246 37.59188353216554 23.523564773877983 3 23.22980843430209 28.501212729448827 32.3715245327506 15.897454303498487 4 10.770370349916055 17.75743902977821 43.672682689758766 27.799507930546962 5 9.76639556777829 42.32981634367947 35.9495216125355 11.954266476006747 6 23.43890029148485 40.64437123786199 18.319460494316907 17.59726797633626 7 22.174210316066755 37.81630995805877 23.579058774990358 16.43042095088412 8 21.689902562411252 30.05123718552884 22.49150991694139 25.767350335118515 9 22.457867436112917 22.22452209743099 26.411145931221775 28.906464535234317 10-11 22.350008883123962 26.062637235640818 28.989341976124233 22.598011905110987 12-13 28.774042508562708 25.892287905676366 27.128015577089204 18.205654008671722 14-15 23.939590684350044 27.611350222790144 31.26627458177688 17.18278451108293 16-17 17.124035048561073 30.761049442150522 32.34033216718684 19.774583342101568 18-19 16.361679299245495 33.93325720436488 32.26893075598826 17.436132740401366 20-21 19.359845714364575 29.529242509970604 29.33403944081807 21.77687233484675 22-23 17.941667454527764 28.922633880603506 30.288543487517174 22.847155177351556 24-25 21.33954472637822 29.412607163094968 30.225117559321475 19.022730551205342 26-27 21.030362703491612 26.13575851195524 34.81849295330861 18.015385831244537 28-29 17.37469417614764 30.732389894786245 33.54100023810263 18.351915690963487 30-31 18.0827770194642 30.170889683546314 31.701161116869386 20.0451721801201 32-33 18.40323250611684 30.491251505922257 31.80742411048812 19.298091877472785 34-35 20.870765455189357 27.656308793908675 31.14302967086478 20.329896080037184 36-37 20.4547863882116 26.935165033892282 34.11805585869105 18.491992719205072 38-39 20.355047744133422 28.215884046646515 33.50201114520458 17.927057064015482 40-41 17.967367316967938 29.726532728083022 34.145854983013265 18.160244971935782 42-43 16.82630325039297 31.069036298906745 30.552717135658302 21.551943315041978 44-45 18.466301363409308 29.17258742925396 30.34169410828985 22.01941709904688 46-47 20.40210105735299 29.92285060952903 29.65794944257132 20.017098890546663 48-49 19.793140579591643 28.79959449927035 30.97046469053199 20.43680023060602 50-51 17.82016803753321 29.864959570320554 33.47094540079269 18.843926991353545 52-53 18.2394131492646 31.49313102771632 30.856017998529683 19.41143782448939 54-55 16.46055265736899 28.486412202845422 32.66721424090241 22.38582089888318 56-57 19.861839416462896 29.524975582047787 29.367453813703147 21.245731187786173 58-59 21.081414821104303 27.896571402451947 30.096910203793303 20.92510357265045 60-61 19.078196258116957 28.190996245377686 32.87236568899708 19.858441807508274 62-63 19.77328256067123 28.140969645161718 31.991453068423255 20.094294725743804 64-65 18.784753792078767 30.308771676941497 31.614724242693043 19.291750288286693 66-67 18.39001160466252 28.508903087380787 30.202892745405347 22.898192562551348 68-69 17.090242716579297 29.728908324666826 31.225843654884255 21.955005303869623 70-71 20.237818836577002 29.10414918117659 29.224049684550785 21.43398229769562 72-73 19.07404532753803 26.64150164101654 31.92837461746574 22.356078413979688 74-75 17.92286427120467 31.151838900335832 30.035204692018713 20.89009213644079 76-77 19.68023600209786 27.183819611923983 30.7111724795393 22.42477190643886 78-79 18.88896815936043 27.534856111395484 32.37017401561659 21.206001713627494 80-81 20.19670327881514 28.731167914028816 30.37537236435651 20.696756442799536 82-83 19.105794689828983 27.673963314014465 31.785064572488697 21.435177423667852 84-85 18.84768043549268 27.653549721876715 30.940700630301947 22.55806921232866 86-87 19.531458390925007 28.305658152452175 30.966133670881163 21.196749785741652 88-89 20.218657043733486 26.907662662423377 30.167666861370314 22.706013432472826 90-91 19.590992777619558 25.558447371830983 31.99435541484513 22.85620443570432 92-93 20.796139768807436 28.228685506324464 31.059969815525463 19.915204909342634 94-95 19.975912264094724 27.297135666064705 30.745715002446193 21.981237067394378 96-97 18.348629257226886 26.51272685315601 31.563656094825372 23.57498779479173 98-99 20.416811460236364 27.414680352964858 29.206355695696868 22.962152491101907 100-101 20.74242948073516 25.458679206196233 30.90501195500125 22.893879358067355 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9095.0 1 5452.5 2 2045.5 3 2174.5 4 1684.0 5 1646.0 6 2963.5 7 5176.0 8 7166.5 9 7416.5 10 5698.0 11 4155.0 12 3831.5 13 3703.5 14 3431.0 15 3226.0 16 3402.5 17 3476.5 18 3227.0 19 3178.5 20 3420.5 21 3837.0 22 4326.0 23 5010.5 24 5546.5 25 6623.0 26 9566.5 27 12613.0 28 13208.0 29 14102.5 30 17635.5 31 21266.5 32 26737.5 33 34528.0 34 41639.0 35 46843.5 36 54528.5 37 62217.0 38 59991.5 39 53768.5 40 48647.0 41 43123.5 42 37430.5 43 35377.5 44 36093.5 45 35968.0 46 45269.5 47 80396.5 48 177602.0 49 176504.5 50 71708.0 51 28227.5 52 13919.0 53 8724.5 54 6068.0 55 4654.0 56 3846.5 57 3413.0 58 2987.5 59 2599.0 60 2313.5 61 1951.5 62 1610.0 63 1285.5 64 986.5 65 804.5 66 634.0 67 542.0 68 504.5 69 409.0 70 302.5 71 225.0 72 180.0 73 169.5 74 152.5 75 107.0 76 79.0 77 54.0 78 39.0 79 33.5 80 21.0 81 12.5 82 8.5 83 4.5 84 5.5 85 6.0 86 5.0 87 4.0 88 3.0 89 2.0 90 1.0 91 2.0 92 1.5 93 0.5 94 2.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15765147575849944 2 0.09137716770779168 3 0.08523938059383435 4 0.10054936642864927 5 0.11937651117146218 6 0.038550820187664564 7 0.043102437598014944 8 0.04799887450914944 9 0.046895452106640256 10-11 0.0452748004529549 12-13 0.05006779151385416 14-15 0.04744716330789485 16-17 0.04644718675562091 18-19 0.050964322215892865 20-21 0.0533780587213817 22-23 0.06641223585102142 24-25 0.08213600508677726 26-27 0.0850669708434423 28-29 0.074412048269213 30-31 0.0628950769430234 32-33 0.05213670851855887 34-35 0.2969585540752837 36-37 0.4679200625640503 38-39 0.44505852966206305 40-41 0.421610803608743 42-43 0.4515066543267261 44-45 0.45978232234554506 46-47 0.45967887649530975 48-49 0.47447163307894846 50-51 0.4638167105047192 52-53 0.4692993405671867 54-55 0.461954685200485 56-57 0.44302409460743675 58-59 0.42205906895976236 60-61 0.3917839167909166 62-63 0.44654125351543483 64-65 0.48454036250184473 66-67 0.4882299311602349 68-69 0.49788487718219027 70-71 0.49454012802458425 72-73 0.48454036250184473 74-75 0.47602332083247706 76-77 0.45909268334397674 78-79 0.43536910169002935 80-81 0.4405069122517127 82-83 0.45102390702562833 84-85 0.4496101470724135 86-87 0.4608857447480542 88-89 0.46998897956875496 90-91 0.47454059697910533 92-93 0.4764026222833396 94-95 0.48109216749400363 96-97 0.4829197108481594 98-99 0.47498886233012466 100-101 0.4624029505515043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1450034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.320333198132623 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2568322999346 13.554344104652547 2 8.661355611478458 3.0003513027664526 3 3.1516066014992425 1.6376062934220381 4 1.779479390897061 1.2328470387818875 5 1.0816475134923873 0.93672476683099 6 0.8031898642304816 0.8346909641900907 7 0.601588346745749 0.7293797429625082 8 0.4646618960830374 0.6438479091707426 9 0.39477595843742724 0.6153886024873557 >10 3.4658910432739445 12.704184136843649 >50 0.6686882556991817 8.193385157024565 >100 0.5933262514716774 20.212873152326203 >500 0.04465896516453486 5.146108330141874 >1k 0.020335778780279267 7.31717997490641 >5k 0.007576074447555021 9.765333291601069 >10k+ 0.004386148364373959 13.47575523189165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38154 2.6312486465834595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 26044 1.7960958156843219 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22923 1.5808594832948744 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18626 1.2845216043210022 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18048 1.2446604700303578 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14373 0.99121813695403 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13858 0.9557017283732658 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12377 0.8535661922410095 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10278 0.7088109658118361 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10256 0.707293760008386 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10185 0.7023973230972514 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9469 0.6530191705849656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9295 0.6410194519576782 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9116 0.6286749138296067 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9084 0.6264680690245884 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8864 0.6112960109900871 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8737 0.6025375956701705 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8483 0.5850207650303373 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8212 0.5663315480878379 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7981 0.5504008871516116 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7112 0.4904712579153317 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6953 0.4795059977903966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6604 0.45543759663566513 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6563 0.45261007672923526 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6524 0.4499204846231192 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6250 0.4310243759801494 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5810 0.4006802599111469 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5465 0.37688771435704266 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5442 0.3753015446534357 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5433 0.3746808695520243 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4886 0.3369576161662416 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4647 0.3204752440287607 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4554 0.3140616013141761 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4113 0.2836485213450167 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4107 0.28323473794407583 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4063 0.2802003263371755 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3826 0.26385588200000826 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3488 0.24054608374700182 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3397 0.2342703688327308 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3300 0.22758087051751888 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3273 0.22571884521328467 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3204 0.22096033610246382 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2663 0.18365086611762207 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2578 0.17778893460429204 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2560 0.1765475844014692 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.16999601388657096 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2377 0.16392719067277042 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2135 0.14723792683481904 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.1405484285196071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.13365203850392474 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1834 0.12647979288761504 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1690 0.1165489912650324 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1686 0.1162731356644051 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1683 0.11606624396393464 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 0.10854917884684083 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1542 0.10634233404182246 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.896390015682391E-5 0.0 12-13 0.0 0.0 0.0 1.3792780031364783E-4 0.0 14-15 0.0 0.0 0.0 5.172292511761794E-4 0.0 16-17 0.0 0.0 0.0 6.896390015682392E-4 0.0 18-19 0.0 0.0 0.0 0.0010689404524307706 0.0 20-21 0.0 0.0 0.0 0.0016896155538421857 0.0 22-23 0.0 0.0 0.0 0.003999906209095787 0.0 24-25 0.0 0.0 0.0 0.009103234820700756 0.0 26-27 0.0 0.0 0.0 0.017068565288813918 0.0 28-29 3.448195007841196E-5 0.0 0.0 0.044412751700994596 0.0 30-31 6.896390015682391E-5 0.0 0.0 0.10982501099974207 0.0 32-33 6.896390015682391E-5 0.0 0.0 0.24627008746001816 0.0 34-35 6.896390015682391E-5 0.0 0.0 0.47740259883561353 0.0 36-37 6.896390015682391E-5 0.0 0.0 0.7968433843620218 0.0 38-39 6.896390015682391E-5 0.0 0.0 1.1994891154276384 0.0 40-41 6.896390015682391E-5 0.0 0.0 1.6123759856665432 0.0 42-43 6.896390015682391E-5 0.0 0.0 2.0327799210225415 0.0 44-45 6.896390015682391E-5 0.0 0.0 2.5149065470188976 0.0 46-47 6.896390015682391E-5 0.0 0.0 3.123719857603339 0.0 48-49 6.896390015682391E-5 0.0 0.0 3.6890859110889815 0.0 50-51 6.896390015682391E-5 0.0 0.0 4.259486329286072 0.0 52-53 6.896390015682391E-5 0.0 0.0 4.770750203098686 0.0 54-55 6.896390015682391E-5 0.0 0.0 5.335323171732525 0.0 56-57 6.896390015682391E-5 0.0 0.0 6.057133832723922 0.0 58-59 6.896390015682391E-5 0.0 0.0 6.777254878161477 0.0 60-61 6.896390015682391E-5 0.0 0.0 7.655096363257689 0.0 62-63 1.3792780031364783E-4 0.0 0.0 8.427388599163882 0.0 64-65 1.3792780031364783E-4 0.0 0.0 9.134923732822816 0.0 66-67 1.3792780031364783E-4 0.0 0.0 9.96366292100737 0.0 68-69 1.3792780031364783E-4 0.0 0.0 10.868055507663957 0.0 70-71 1.3792780031364783E-4 0.0 0.0 11.837136232667648 0.0 72-73 1.3792780031364783E-4 0.0 0.0 12.775665949901864 0.0 74-75 1.3792780031364783E-4 0.0 0.0 13.797676468275917 0.0 76-77 1.3792780031364783E-4 0.0 0.0 14.705206912389642 0.0 78-79 1.3792780031364783E-4 0.0 0.0 15.890179126834266 0.0 80-81 1.3792780031364783E-4 0.0 0.0 17.11021948450864 0.0 82-83 1.3792780031364783E-4 0.0 0.0 18.17029807576926 0.0 84-85 1.3792780031364783E-4 0.0 0.0 19.439026946954346 0.0 86-87 1.3792780031364783E-4 0.0 0.0 20.72944496473876 0.0 88-89 1.3792780031364783E-4 0.0 0.0 21.922658365252126 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 380 0.0 41.14572 3 GACATAT 445 0.0 35.157516 1 CATTCCG 385 0.0 33.2057 9 CTTTAGG 350 0.0 32.48909 2 GGCATTC 395 0.0 32.36505 7 TGTATTG 60 0.0040005483 31.586617 5 TAGGCAT 360 0.0 31.586615 5 GTACCCA 250 0.0 30.342003 1 GCTTTGC 585 0.0 29.985422 1 GGATATG 545 0.0 29.538744 9 CTTATGT 265 0.0 28.606745 2 GTAAGCG 150 0.0 28.531368 94-95 CCTTTAG 385 0.0 28.322487 1 GCTTATG 285 0.0 28.279282 1 ACATATA 570 0.0 27.430483 2 CTTTGCG 665 0.0 27.074242 2 GTGTACG 70 0.008523745 27.056496 9 AGGCTTA 625 0.0 26.514452 6 GTATGGT 90 9.048994E-4 26.338545 1 CATGGGG 1455 0.0 26.050817 4 >>END_MODULE