##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938805_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1450034 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8811779585858 31.0 31.0 34.0 30.0 34.0 2 32.07114660759679 33.0 31.0 34.0 30.0 34.0 3 32.150326819922846 34.0 31.0 34.0 30.0 34.0 4 35.75802774279776 37.0 35.0 37.0 35.0 37.0 5 35.68151712304677 37.0 35.0 37.0 33.0 37.0 6 35.77077916793675 37.0 35.0 37.0 33.0 37.0 7 35.72631124511563 37.0 35.0 37.0 33.0 37.0 8 35.73623101251419 37.0 35.0 37.0 33.0 37.0 9 37.30469768295088 39.0 37.0 39.0 34.0 39.0 10-11 37.22871463703609 39.0 37.0 39.0 33.0 39.0 12-13 37.20713445339902 39.0 37.0 39.0 33.0 39.0 14-15 38.19351339347905 40.0 37.0 41.0 33.0 41.0 16-17 38.18618356535088 40.0 37.5 41.0 33.0 41.0 18-19 38.345131907251826 40.0 37.5 41.0 33.5 41.0 20-21 38.429519583678726 40.0 38.0 41.0 34.0 41.0 22-23 38.389066739124736 40.0 38.0 41.0 34.0 41.0 24-25 38.26388070900407 40.0 37.5 41.0 33.0 41.0 26-27 37.98911128980424 40.0 37.0 41.0 33.0 41.0 28-29 37.84425434162233 40.0 37.0 41.0 33.0 41.0 30-31 37.70201009079787 40.0 37.0 41.0 33.0 41.0 32-33 37.44989807135557 40.0 36.0 41.0 32.0 41.0 34-35 37.34368538944604 39.5 36.0 41.0 32.0 41.0 36-37 37.180832656337714 39.5 35.5 41.0 31.5 41.0 38-39 37.00981218371432 39.0 35.0 41.0 31.0 41.0 40-41 36.93838696196089 39.0 35.0 41.0 31.0 41.0 42-43 36.78674672455956 39.0 35.0 41.0 31.0 41.0 44-45 36.55093397809982 39.0 35.0 41.0 30.0 41.0 46-47 36.21847522196032 39.0 35.0 41.0 30.0 41.0 48-49 35.90206160683129 39.0 35.0 41.0 28.5 41.0 50-51 35.68793042094185 39.0 35.0 41.0 28.0 41.0 52-53 35.517042703826256 39.0 35.0 41.0 27.5 41.0 54-55 35.36938754539548 39.0 35.0 40.0 27.0 41.0 56-57 35.14067049462288 38.5 35.0 40.0 26.5 41.0 58-59 34.90610737403399 38.0 34.5 40.0 26.0 41.0 60-61 34.448086389698446 38.0 34.0 40.0 25.0 41.0 62-63 34.11190772078447 38.0 34.0 40.0 23.5 41.0 64-65 33.680816105001675 37.0 33.0 39.5 22.0 41.0 66-67 33.19746019748502 36.5 33.0 39.0 22.0 40.5 68-69 32.70797098550793 36.0 32.0 39.0 21.0 40.0 70-71 32.098335970053114 35.5 31.5 37.5 19.0 39.5 72-73 31.688031797875084 35.0 31.0 37.0 18.0 39.0 74-75 31.272279822404165 35.0 31.0 37.0 15.0 39.0 76-77 29.146767593035747 32.5 28.5 34.5 8.5 36.5 78-79 30.192548588515855 34.0 30.0 36.0 7.5 37.0 80-81 30.205738968879352 34.0 31.0 35.5 6.5 37.0 82-83 29.89387041959016 34.0 30.0 35.0 2.0 37.0 84-85 29.529354828921253 34.0 30.0 35.0 2.0 36.0 86-87 29.093251951333556 34.0 29.0 35.0 2.0 36.0 88-89 28.528867598966645 34.0 29.0 35.0 2.0 35.0 90-91 28.072389337077613 33.5 28.5 35.0 2.0 35.0 92-93 27.8683806724532 34.0 28.0 35.0 2.0 35.0 94-95 27.513827606800945 33.0 27.0 35.0 2.0 35.0 96-97 26.994110482926608 33.0 26.0 35.0 2.0 35.0 98-99 26.347185307379 33.0 25.0 35.0 2.0 35.0 100-101 23.37700633226531 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0644428884899888 1101 2 -0.0019583887868890315 1101 3 -0.018575951376760713 1101 4 0.08341295527109338 1101 5 -0.034907927184107734 1101 6 0.02175859784313161 1101 7 -0.013627736940208024 1101 8 0.08550179752147358 1101 9 0.10344365356398555 1101 10-11 -0.015609219518033512 1101 12-13 0.14930750367100387 1101 14-15 0.255489650930663 1101 16-17 0.041095856605601 1101 18-19 0.14607482247008363 1101 20-21 0.12068522506483248 1101 22-23 0.1717562978873275 1101 24-25 0.15697616802606262 1101 26-27 0.04908406060877013 1101 28-29 0.21597566158557413 1101 30-31 0.2305243253989957 1101 32-33 0.28615638681765887 1101 34-35 0.265339034172996 1101 36-37 0.08537874626831155 1101 38-39 0.20537538040410652 1101 40-41 0.338519226560436 1101 42-43 0.3079360515354779 1101 44-45 0.2892214147385559 1101 46-47 0.2355981670254863 1101 48-49 -0.047176719337628015 1101 50-51 0.18693703722477295 1101 52-53 0.376923246473055 1101 54-55 0.5277263795090334 1101 56-57 0.36067719801376796 1101 58-59 0.4228235015841406 1101 60-61 0.6895297012705797 1101 62-63 0.47746635338421584 1101 64-65 0.41523591608225985 1101 66-67 0.35696768388496025 1101 68-69 0.3693985798146642 1101 70-71 0.4324577717023885 1101 72-73 0.4257244626867198 1101 74-75 0.6297443648421819 1101 76-77 0.5062460688192978 1101 78-79 0.6899776699460709 1101 80-81 0.7605766875786806 1101 82-83 0.8096963369749766 1101 84-85 0.6445505848353896 1101 86-87 0.7780398693547461 1101 88-89 0.9970782973728589 1101 90-91 0.9950848419856406 1101 92-93 1.0050051448833734 1101 94-95 0.9227816371212043 1101 96-97 0.6975444987716557 1101 98-99 0.7252756084985386 1101 100-101 1.1673399799593618 1102 1 0.08798131027768719 1102 2 0.06496501207307404 1102 3 0.05793231583366776 1102 4 0.11107874276167706 1102 5 0.15945962013625348 1102 6 0.1256129353580917 1102 7 0.13731035872005748 1102 8 0.14612394389321537 1102 9 0.3018079427612079 1102 10-11 0.22163644739982402 1102 12-13 0.16083727006136428 1102 14-15 0.27328299283411894 1102 16-17 0.31260972025203415 1102 18-19 0.21773136752970856 1102 20-21 0.2167075997268313 1102 22-23 0.1925135344669684 1102 24-25 0.150124135577812 1102 26-27 0.20766129142574385 1102 28-29 0.2716319406759595 1102 30-31 0.15792940762781882 1102 32-33 0.2262759067431901 1102 34-35 0.27209880854243096 1102 36-37 0.3080966748112459 1102 38-39 0.27609189044444804 1102 40-41 0.257654050799097 1102 42-43 0.3398493123183144 1102 44-45 0.12829201848872884 1102 46-47 0.22795653840719865 1102 48-49 0.23492161170541692 1102 50-51 0.40017759531689734 1102 52-53 0.4389328656937579 1102 54-55 0.47101536616624173 1102 56-57 0.338869660002338 1102 58-59 0.17059877974363502 1102 60-61 0.12899953592636138 1102 62-63 -0.08336607491491321 1102 64-65 0.014752461585160859 1102 66-67 0.04369498784347314 1102 68-69 0.10099378571918294 1102 70-71 0.04190411240897873 1102 72-73 -0.007722554025015427 1102 74-75 0.06483346453631711 1102 76-77 0.245081178974889 1102 78-79 -0.0061649449748024665 1102 80-81 0.018828106081276275 1102 82-83 0.15483491802910265 1102 84-85 0.22234187655931592 1102 86-87 0.1334847583125871 1102 88-89 0.24142174123118565 1102 90-91 0.12500444170193603 1102 92-93 -0.0699183889612165 1102 94-95 0.09624898939745563 1102 96-97 0.11166885047164143 1102 98-99 0.41443209814790194 1102 100-101 0.7342090787669342 1103 1 0.03441541023345707 1103 2 0.04445417615976055 1103 3 0.017176013312656835 1103 4 0.015308066069941617 1103 5 -0.09729071157538272 1103 6 -0.06428091691966387 1103 7 0.021592755889450643 1103 8 0.07234021987729022 1103 9 0.059614223168104274 1103 10-11 -0.07817558267535674 1103 12-13 0.044671856527521925 1103 14-15 0.004751369658528404 1103 16-17 -0.0734113987218663 1103 18-19 0.03851862980303622 1103 20-21 -0.010340719600904436 1103 22-23 -0.06756497061928002 1103 24-25 0.042019314702102406 1103 26-27 0.023702313097565764 1103 28-29 0.06988492608064689 1103 30-31 0.06591536516872765 1103 32-33 0.13927070992762225 1103 34-35 -0.07791501277556989 1103 36-37 0.00792805473162872 1103 38-39 0.10210637518570564 1103 40-41 0.17608063195788048 1103 42-43 0.39969285499854124 1103 44-45 0.4198527217173833 1103 46-47 0.39339771487779274 1103 48-49 0.3581994754825075 1103 50-51 0.4016675333973936 1103 52-53 0.29051402447306174 1103 54-55 0.1683533803192745 1103 56-57 0.20782974403594778 1103 58-59 0.14705278239733133 1103 60-61 0.23824323340535614 1103 62-63 0.15902943592100627 1103 64-65 0.30695447396906417 1103 66-67 0.3137994770075565 1103 68-69 0.2656029188461204 1103 70-71 0.2569859346115422 1103 72-73 0.18238967507715032 1103 74-75 0.2282898997419771 1103 76-77 0.06850278445919145 1103 78-79 0.04303618284474808 1103 80-81 0.07850026441829527 1103 82-83 -0.027802742298188576 1103 84-85 -0.02634509823060327 1103 86-87 0.16986523155451394 1103 88-89 0.19945745551689953 1103 90-91 0.33457155802215155 1103 92-93 0.2705283160365717 1103 94-95 -0.008461315777246625 1103 96-97 0.05107895661936368 1103 98-99 0.04822522066006485 1103 100-101 0.700042780668749 1104 1 -0.05601079876105075 1104 2 -0.04289008118374227 1104 3 -0.0817376985135354 1104 4 -0.1307971539384667 1104 5 0.0031289171233410684 1104 6 -0.018648183723684042 1104 7 -0.03885535146645225 1104 8 -0.07801522396905369 1104 9 -0.077962502002066 1104 10-11 0.04613429531948299 1104 12-13 -0.17065483897738432 1104 14-15 -0.11545445336387417 1104 16-17 -0.016828945458151168 1104 18-19 0.10223638905050336 1104 20-21 -0.011959903859974474 1104 22-23 -0.06543122880420071 1104 24-25 -0.11820441535475368 1104 26-27 -0.14371065979377562 1104 28-29 0.07270798084812213 1104 30-31 -0.03090667558595328 1104 32-33 -0.07766060688665277 1104 34-35 -0.10574373091237987 1104 36-37 -0.16536996794341974 1104 38-39 -0.004763561492431734 1104 40-41 -0.08099372538455185 1104 42-43 0.0652169593771319 1104 44-45 -0.09108567161170811 1104 46-47 -0.06664532672910184 1104 48-49 -0.0010091630435056231 1104 50-51 -0.03577111343489747 1104 52-53 0.02124993886449289 1104 54-55 -0.12309331389113964 1104 56-57 -0.1446400243592123 1104 58-59 -0.13670035942422487 1104 60-61 -0.08620849850692025 1104 62-63 -0.3045550993051549 1104 64-65 -0.05928806926132779 1104 66-67 -0.12060737944491251 1104 68-69 0.082638333782306 1104 70-71 -0.04166834096261596 1104 72-73 -0.16838431873089732 1104 74-75 -0.3308265067692808 1104 76-77 -0.316280011842899 1104 78-79 -0.12134607840378564 1104 80-81 -0.12436593982977229 1104 82-83 -0.2710169471071957 1104 84-85 -0.17659105026995192 1104 86-87 0.14593992330541283 1104 88-89 0.3475103352053601 1104 90-91 0.27035056795731904 1104 92-93 0.36494531261697816 1104 94-95 0.2963924614204103 1104 96-97 0.3676677744314709 1104 98-99 0.4701440780618178 1104 100-101 0.7474802408903223 1105 1 -0.10595065644839607 1105 2 -0.0707429074583743 1105 3 -0.011972615835972533 1105 4 -0.06502154583861142 1105 5 -0.1025310852368051 1105 6 0.014658856756952332 1105 7 -0.004548354462180271 1105 8 -0.006318548255158873 1105 9 -0.05848405282490887 1105 10-11 -0.07984262907924489 1105 12-13 -0.07790731868323064 1105 14-15 -0.14865487322139614 1105 16-17 -0.14045400523816198 1105 18-19 -0.2684115744955875 1105 20-21 -0.06452542378561077 1105 22-23 -0.015723245093340665 1105 24-25 -0.08825333662318258 1105 26-27 -0.03610250197041154 1105 28-29 -0.1360541640689661 1105 30-31 -0.30672077062530434 1105 32-33 -0.2429727775790269 1105 34-35 -0.1995828186539086 1105 36-37 -0.2976312981961442 1105 38-39 -0.13241173827977093 1105 40-41 -0.18999364464271906 1105 42-43 -0.12829377825124766 1105 44-45 -0.169499446582158 1105 46-47 -0.1092718377917663 1105 48-49 -0.3293147646633088 1105 50-51 -0.3063525167279195 1105 52-53 -0.4318866742434224 1105 54-55 -0.45952692650840277 1105 56-57 -0.5160519298457231 1105 58-59 -0.5321376178456987 1105 60-61 -0.5005735054113813 1105 62-63 -0.4704039961440074 1105 64-65 -0.676838341402707 1105 66-67 -0.6243717085320455 1105 68-69 -0.5139216501521346 1105 70-71 -0.4206627325108059 1105 72-73 -0.5125826041056527 1105 74-75 -0.7160064471859471 1105 76-77 -0.5848351057208028 1105 78-79 -0.7628839204437803 1105 80-81 -0.5920055455611966 1105 82-83 -0.46172087095315817 1105 84-85 -0.4099670318525348 1105 86-87 -0.44345215281549955 1105 88-89 -0.2970071909477454 1105 90-91 -0.30577552179861556 1105 92-93 -0.2382360190435513 1105 94-95 -0.217582603845905 1105 96-97 -0.16805220461705872 1105 98-99 -0.058910041738354124 1105 100-101 0.19299865520357073 1106 1 0.001810155068614705 1106 2 0.03234630193841781 1106 3 -0.011203049155703582 1106 4 -0.06345173207886745 1106 5 -0.04138198156466899 1106 6 -0.04352242963127395 1106 7 0.013027934907164251 1106 8 0.04500771426883432 1106 9 0.014706596198486466 1106 10-11 0.004609516145400505 1106 12-13 0.1364573976304584 1106 14-15 -0.015944505394095643 1106 16-17 -0.03207933006688535 1106 18-19 -0.15328209809751314 1106 20-21 -0.03870965753752387 1106 22-23 0.022341670540669156 1106 24-25 -0.03968805435840039 1106 26-27 0.05389180170214303 1106 28-29 -0.018197967190801023 1106 30-31 -0.009403157928531414 1106 32-33 -0.056878770790476096 1106 34-35 -0.08496621626267853 1106 36-37 -0.027494218172456897 1106 38-39 0.01208143686542229 1106 40-41 0.10555447602943246 1106 42-43 0.13183637822901773 1106 44-45 0.38362954506561664 1106 46-47 0.35463088284377164 1106 48-49 0.22653660107465612 1106 50-51 0.32695381785409694 1106 52-53 0.15199098050595694 1106 54-55 0.22329654972400448 1106 56-57 0.3517904608527971 1106 58-59 0.3099945983120236 1106 60-61 0.11978621204259099 1106 62-63 0.11489313840897353 1106 64-65 0.06271932905503519 1106 66-67 0.07731919053659198 1106 68-69 0.14129764822803992 1106 70-71 -0.058733733090669205 1106 72-73 0.13175742471347007 1106 74-75 0.2781041945150555 1106 76-77 0.2638223200096199 1106 78-79 0.17821226976651516 1106 80-81 0.08869433570565732 1106 82-83 0.32176883014816937 1106 84-85 0.1816482195784488 1106 86-87 0.3999934400985552 1106 88-89 0.349735880139761 1106 90-91 0.27891567486990354 1106 92-93 0.5738094636256044 1106 94-95 0.49933190931241 1106 96-97 0.4577310404078574 1106 98-99 0.43409457157100917 1106 100-101 0.8784330334090313 1107 1 -0.1858378878082796 1107 2 -0.05522102868263801 1107 3 -0.15381176468773816 1107 4 -0.12284481965066618 1107 5 -0.25719432755783345 1107 6 -0.23229070295558074 1107 7 -0.17363655160087177 1107 8 -0.17863680191983633 1107 9 -0.12564028758282575 1107 10-11 -0.1874448990237383 1107 12-13 -0.2156704438236403 1107 14-15 -0.29037512118288333 1107 16-17 -0.3303792039291338 1107 18-19 -0.2895081341615793 1107 20-21 -0.24658596103548547 1107 22-23 -0.3312664754332104 1107 24-25 -0.3608753694135203 1107 26-27 -0.15323353985881027 1107 28-29 -0.07148743891318077 1107 30-31 -0.26750320931797944 1107 32-33 -0.2848901480377677 1107 34-35 -0.18621785262414647 1107 36-37 -0.10347879338444699 1107 38-39 -0.35456616169646793 1107 40-41 -0.4787528050708758 1107 42-43 -0.4121157689456112 1107 44-45 -0.3844054693423118 1107 46-47 -0.5474858755850391 1107 48-49 -0.8441885070966606 1107 50-51 -0.7636089115719784 1107 52-53 -0.7626284741296843 1107 54-55 -0.8280807273903932 1107 56-57 -0.7234565713317167 1107 58-59 -0.6638362916201146 1107 60-61 -0.7136702107941986 1107 62-63 -0.7611004821420337 1107 64-65 -0.6008738274999033 1107 66-67 -0.7293945880991117 1107 68-69 -0.692026076503403 1107 70-71 -0.9198977191230711 1107 72-73 -0.5707438600228691 1107 74-75 -0.5321325448688476 1107 76-77 -0.5705791419557258 1107 78-79 -0.5023518313703157 1107 80-81 -0.5650433772946819 1107 82-83 -0.45947237922360173 1107 84-85 -0.435120894664184 1107 86-87 -0.46654978916699363 1107 88-89 -0.18390259256965535 1107 90-91 -0.0886655318976004 1107 92-93 -0.05859094073086979 1107 94-95 -0.1093033577686171 1107 96-97 -0.0015246772900923133 1107 98-99 0.06206275980947851 1107 100-101 0.46490374092352127 1108 1 -0.1454529457776772 1108 2 -0.0570938114563333 1108 3 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-1.0277915432913218 2316 56-57 -0.993307468548096 2316 58-59 -0.9455422292456674 2316 60-61 -0.9547923771200786 2316 62-63 -0.91354923620964 2316 64-65 -0.9557966805004909 2316 66-67 -0.9343365516171716 2316 68-69 -0.8821997662512864 2316 70-71 -0.9345288779864802 2316 72-73 -1.1518070893498944 2316 74-75 -1.1464843397498363 2316 76-77 -1.1281207470599064 2316 78-79 -1.2069384778045524 2316 80-81 -1.161404188754151 2316 82-83 -1.3025964231883833 2316 84-85 -1.3917182201696754 2316 86-87 -1.6331888155329857 2316 88-89 -1.8304006004306395 2316 90-91 -2.478413330113355 2316 92-93 -2.5501077453545555 2316 94-95 -2.4057932569025766 2316 96-97 -2.4250341180487105 2316 98-99 -2.9787616247218374 2316 100-101 -3.6184586588685015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 46.0 6 212.0 7 397.0 8 718.0 9 1262.0 10 2110.0 11 3145.0 12 4434.0 13 6162.0 14 7164.0 15 9392.0 16 12081.0 17 16194.0 18 15074.0 19 9968.0 20 8800.0 21 9582.0 22 10117.0 23 11338.0 24 12840.0 25 14629.0 26 17302.0 27 20632.0 28 25404.0 29 31155.0 30 37449.0 31 45261.0 32 55364.0 33 69077.0 34 90225.0 35 130055.0 36 183651.0 37 304060.0 38 260573.0 39 24140.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61852846888743 21.831027352643716 15.456704627029227 18.093739551439626 2 13.637611256011928 26.42420798408865 36.95451279073456 22.983667969164863 3 23.46089815825008 28.503400609916735 32.26703649707524 15.768664734757943 4 10.960432651924023 18.53791014555521 42.77837623117803 27.72328097134274 5 9.53674189708655 43.05974894381787 35.7255071260398 11.678002033055776 6 23.39448592239906 41.43213193621667 17.718343156091514 17.45503898529276 7 22.4039574244466 37.80380322116585 23.065183299150227 16.727056055237323 8 22.118998588998604 30.748382451721824 21.817695309213438 25.314923650066135 9 22.285960191278274 22.458300977770175 25.737258574626527 29.518480256325024 10-11 22.51964436695967 26.539239769550232 28.735739989545074 22.205375873945023 12-13 28.893219055553182 26.000183443974418 26.843646424842454 18.262951075629953 14-15 23.63308094792456 28.293601998853816 30.793651997784877 17.279665055436748 16-17 17.225705161604672 31.153282627743568 31.859418367322306 19.761593843329454 18-19 16.5515025018422 34.27615193187182 31.621313054233653 17.551032512052327 20-21 19.51395622447474 29.787508430836795 28.93794214480488 21.760593199883587 22-23 18.119747536954307 29.335829366759675 29.77802589456523 22.766397201720785 24-25 21.46353119995807 30.00477919828087 29.862782527857966 18.668907073903096 26-27 21.26335099385703 26.546715332242556 34.339919967311076 17.850013706589333 28-29 17.411833101844508 31.301231557329 33.05784554017354 18.22908980065295 30-31 18.36874169847052 30.488457512030752 31.136511281804424 20.0062895076943 32-33 18.450981149407532 30.913309618946865 31.434262920731516 19.20144631091409 34-35 20.882646670970377 27.99838886773818 30.919704092386297 20.199260368905147 36-37 20.580209674782672 27.4160228284644 33.64477375361179 18.35899374314115 38-39 20.17111153873477 28.64554343725278 33.369267515154775 17.81407750885767 40-41 18.033218233163538 29.9997965372011 33.61539944402928 18.351585785606076 42-43 16.945911127295197 31.43334685072387 30.301265393273308 21.319476628707626 44-45 18.760758561332075 29.320389956469207 30.001903437774136 21.916948044424583 46-47 20.573526485824424 30.30541481912385 29.216285526011344 19.904773169040382 48-49 19.96439393834903 29.140696011265945 30.638247103171373 20.256662947213652 50-51 17.78061834084987 30.215847928191607 33.0430740700767 18.960459660881828 52-53 18.279295519967118 31.896045196181603 30.487526499378635 19.33713278447264 54-55 16.451993539461835 28.86653002619249 31.982043179677166 22.69943325466851 56-57 19.84915526118698 29.878023549792626 28.955424493494636 21.31739669552576 58-59 21.166211962803477 28.36614189519099 29.443948668577434 21.0236974734281 60-61 18.838498779327182 29.18190783575399 32.00866194949035 19.970931435428476 62-63 19.53161098291488 28.823703444195104 31.25150858531593 20.393176987574087 64-65 18.5332895642447 31.201027010401134 30.615006268818522 19.65067715653564 66-67 18.363314611700837 29.076127435835193 29.322021274248005 23.238536678215965 68-69 17.125256461095496 30.39947587110567 30.091791520835848 22.383476146962984 70-71 20.00194481551494 29.894228242721255 28.17372168034819 21.93010526141562 72-73 18.997657985950674 27.166259549776072 30.753416816433273 23.08266564783998 74-75 17.453935562890248 31.77104812714736 28.76049802970137 22.014518280261015 76-77 19.229443006071083 27.59006439717007 29.657559755582763 23.522932841176083 78-79 18.315262926064193 27.9604556274432 31.173599740335266 22.550681706157345 80-81 19.5689616826094 28.964227378195055 29.28035800552194 22.1864529336736 82-83 18.28357128177684 28.15002958551317 30.67369454785198 22.89270458485801 84-85 17.655241187448016 27.894276961781582 30.027468321432465 24.423013529337933 86-87 18.269255009408774 28.71878001216286 29.622291804298857 23.38967317412951 88-89 18.851090842732876 27.292430262345384 29.040580768026626 24.815898126895117 90-91 18.436147880332797 25.75049611218861 30.651071391399753 25.16228461607884 92-93 19.17234354504791 28.546572011414906 29.879368345845688 22.4017160976915 94-95 18.418568116333827 27.430563697127102 29.641408408354565 24.50945977818451 96-97 16.213137071268672 27.138984327264048 30.552076709925423 26.095801891541853 98-99 18.026458027664898 27.65017335818335 28.355605822628117 25.96776279152364 100-101 18.152574242506265 26.08255630837601 29.784242171791796 25.98062727732593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8071.0 1 4908.0 2 1872.0 3 1940.0 4 1584.5 5 1607.0 6 2832.0 7 5003.5 8 6698.0 9 6835.0 10 5368.0 11 3811.5 12 3370.5 13 3186.5 14 2919.0 15 2684.5 16 2523.5 17 2487.0 18 2598.0 19 2803.0 20 3075.0 21 3445.5 22 3884.0 23 4386.0 24 4826.5 25 5933.5 26 8850.0 27 11966.0 28 12604.5 29 13668.0 30 17452.5 31 21155.0 32 26991.0 33 34574.0 34 41473.0 35 47310.0 36 55111.0 37 61786.0 38 59212.0 39 53436.0 40 48533.5 41 42515.0 42 36386.5 43 34957.5 44 36089.5 45 36390.0 46 43858.5 47 79632.0 48 171356.5 49 163047.5 50 74399.5 51 48675.5 52 29687.0 53 12925.5 54 5669.5 55 4037.5 56 3537.0 57 3162.5 58 2751.5 59 2429.5 60 2126.5 61 1739.0 62 1350.0 63 994.0 64 733.0 65 580.5 66 468.5 67 415.0 68 380.0 69 282.5 70 175.0 71 136.5 72 113.0 73 107.5 74 78.5 75 34.5 76 22.5 77 13.5 78 12.5 79 11.5 80 6.5 81 3.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3763360031557881 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.068917004704717E-4 16-17 0.002034435054626305 18-19 1.7240975039205976E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0344585023523585E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008413595819132518 36-37 0.018275433541558337 38-39 0.011103187925248649 40-41 0.009482536271563287 42-43 0.003448195007841196 44-45 0.0017930614040774218 46-47 0.001551687753528538 48-49 0.0 50-51 5.861931513330032E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.137834009409434E-4 60-61 9.654946021955347E-4 62-63 0.0 64-65 0.0 66-67 0.0012413502028228304 68-69 6.206751014114152E-4 70-71 0.001724097503920598 72-73 0.0 74-75 0.0 76-77 0.09968731767668897 78-79 0.032585442824099295 80-81 3.448195007841196E-5 82-83 0.0 84-85 0.0 86-87 0.150582675992425 88-89 0.3245785960880917 90-91 0.27864863858364697 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.0344585023523585E-4 100-101 3.793014508625315E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1450034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.66366029636531 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8563271652437 11.88187740046325 2 9.563758132247605 2.996069170802553 3 3.528116334260621 1.6578964723774803 4 1.9544849983182881 1.2245755627199915 5 1.2420337896689944 0.9727397678991183 6 0.8971300263740248 0.8431403984875185 7 0.6696379545595553 0.7342287009241648 8 0.520141092716445 0.6517850705992517 9 0.4490982966649998 0.6331070842773161 >10 3.822528803210633 12.710540937452286 >50 0.7375895177216901 8.199639765211503 >100 0.6698157791759098 20.667264097790344 >500 0.053250794533695855 5.651836204400532 >1k 0.02200446055111399 7.042305512221864 >5k 0.008361695009423317 9.252814624011394 >10k+ 0.005721159743289637 14.880179230361431 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 42623 2.9394483163843055 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 23008 1.5867214148082045 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22859 1.5764457936848375 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 19694 1.3581750496884901 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16360 1.1282494065656392 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13070 0.9013581750496885 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12806 0.8831517054082869 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11949 0.8240496429738888 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11220 0.7737749597595642 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11053 0.7622579884333747 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10876 0.7500513781056168 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10203 0.7036386733000743 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10140 0.6992939475901945 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9900 0.6827426115525567 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9555 0.6589500659984524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9417 0.6494330477768108 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8623 0.5946757110522926 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8526 0.5879862127370806 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8001 0.5517801651547481 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7867 0.5425390025337337 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7502 0.517367178976493 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7137 0.49219535541925225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6874 0.47405784967800757 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6513 0.4491618817213941 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6126 0.42247285236070325 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6021 0.4152316428442367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 0.411990339536866 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5478 0.37778424505908137 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5443 0.37537050855359255 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5135 0.3541296273052908 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5119 0.3530262049027816 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5016 0.3459229231866287 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4348 0.29985503788187035 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4277 0.2949586009707359 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4270 0.2944758536696381 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4177 0.28806221095505347 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3794 0.26164903719498994 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3771 0.26006286749138297 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3657 0.252200982873505 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3471 0.23937369744433581 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3432 0.23668410533821965 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3324 0.22923600412128267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2625 0.18103023791166276 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2625 0.18103023791166276 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2602 0.1794440682080558 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2596 0.17903028480711486 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2466 0.17006497778672774 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2094 0.14441040692838927 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2083 0.1436518040266642 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2070 0.14275527332462548 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1994 0.13751401691270687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1849 0.12751425138996741 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1812 0.12496258708416492 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1807 0.12461776758338079 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1760 0.12137646427601008 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1754 0.12096268087506913 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1723 0.1188247999702076 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1624 0.11199737385468203 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1551 0.1069630091432339 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1506 0.1038596336361768 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1481 0.10213553613225622 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1471 0.10144589713068797 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.896390015682391E-5 0.0 12-13 0.0 0.0 0.0 1.3792780031364783E-4 0.0 14-15 0.0 0.0 0.0 5.172292511761794E-4 0.0 16-17 0.0 0.0 0.0 6.896390015682392E-4 0.0 18-19 0.0 0.0 0.0 0.0011379043525875944 0.0 20-21 0.0 0.0 0.0 0.0018275433541558336 0.0 22-23 0.0 0.0 0.0 0.004103352059331023 0.0 24-25 0.0 0.0 0.0 0.009310126521171228 0.0 26-27 0.0 0.0 0.0 0.017344420889441214 0.0 28-29 0.0 0.0 0.0 0.04510239070256283 0.0 30-31 0.0 0.0 0.0 0.11151462655358427 0.0 32-33 0.0 0.0 0.0 0.2500631019686435 0.0 34-35 0.0 0.0 0.0 0.4849886278528641 0.0 36-37 0.0 0.0 0.0 0.8080155361874273 0.0 38-39 0.0 0.0 0.0 1.215454258313943 0.0 40-41 0.0 0.0 0.0 1.6343409878664914 0.0 42-43 0.0 0.0 0.0 2.05908964893237 0.0 44-45 0.0 0.0 0.0 2.5458023742891545 0.0 46-47 0.0 0.0 0.0 3.1615120748892784 0.0 48-49 0.0 0.0 0.0 3.732602132087937 0.0 50-51 0.0 0.0 0.0 4.307381757944986 0.0 52-53 0.0 0.0 0.0 4.82278346576701 0.0 54-55 0.0 0.0 0.0 5.392045979611513 0.0 56-57 0.0 0.0 0.0 6.120132355517181 0.0 58-59 0.0 0.0 0.0 6.846977381220027 0.0 60-61 0.0 0.0 0.0 7.7337496913865476 0.0 62-63 0.0 0.0 0.0 8.51252453390748 0.0 64-65 0.0 0.0 0.0 9.22733535903296 0.0 66-67 0.0 0.0 0.0 10.06290197333304 0.0 68-69 0.0 0.0 0.0 10.971673767649586 0.0 70-71 0.0 0.0 0.0 11.948754305071468 0.0 72-73 0.0 0.0 0.0 12.895076943023405 0.0 74-75 0.0 0.0 0.0 13.925328647466198 0.0 76-77 0.0 0.0 0.0 14.838824468943486 0.0 78-79 0.0 0.0 0.0 16.02652075744431 0.0 80-81 0.0 0.0 0.0 17.25028516572715 0.0 82-83 0.0 0.0 0.0 18.313501614444903 0.0 84-85 0.0 0.0 0.0 19.581023617377248 0.0 86-87 0.0 0.0 0.0 20.869131344506407 0.0 88-89 0.0 0.0 0.0 22.063379203522125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGGTA 15 6.645985E-4 94.90021 8 GTATGGT 120 0.0 47.637367 1 CGGAGTC 25 0.0016237628 37.964012 46-47 GTAAGCG 145 0.0 34.36042 94-95 GCTTTGC 565 0.0 31.196152 1 CTTTGCG 595 0.0 29.50679 2 ATTGTCA 115 3.897245E-6 28.882671 6 GACATAT 305 0.0 28.113857 1 TTGCGTT 625 0.0 28.090462 4 GTATTGA 70 0.008274887 27.221354 1 ACCGATT 70 0.008435415 27.114346 8 ATAGACC 70 0.008435415 27.114346 3 CATGGGG 1545 0.0 26.719475 4 GGATATG 505 0.0 26.4128 1 TTTGCGT 665 0.0 26.400808 3 CTGTACC 90 9.0037566E-4 26.36117 4 GAACCGA 95 0.0012342479 24.97374 6 TATTGTC 115 1.2911587E-4 24.756577 5 CATATAG 345 0.0 24.756575 3 GGGATTC 135 1.3540248E-5 24.603758 7 >>END_MODULE