##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938804_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1446541 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65540485890134 33.0 31.0 34.0 30.0 34.0 2 31.672415783583045 33.0 31.0 34.0 30.0 34.0 3 31.684129243484975 33.0 31.0 34.0 28.0 34.0 4 35.198878566179594 37.0 35.0 37.0 33.0 37.0 5 35.26318576521509 37.0 35.0 37.0 33.0 37.0 6 35.37007385203738 37.0 35.0 37.0 33.0 37.0 7 35.33691544173307 37.0 35.0 37.0 33.0 37.0 8 35.3264193686871 37.0 35.0 37.0 33.0 37.0 9 36.85723391179372 39.0 37.0 39.0 33.0 39.0 10-11 36.74564149927309 39.0 37.0 39.0 32.5 39.0 12-13 36.70802244803293 39.0 37.0 39.0 32.0 39.0 14-15 37.70629349600184 40.0 37.0 41.0 32.0 41.0 16-17 37.65090896144665 40.0 37.0 41.0 32.0 41.0 18-19 37.756733822269815 40.0 37.5 41.0 32.5 41.0 20-21 37.65757728263492 40.0 37.0 41.0 32.0 41.0 22-23 37.685569921626836 40.0 37.0 41.0 32.0 41.0 24-25 37.587845418830156 40.0 37.0 41.0 32.0 41.0 26-27 37.37368902782569 40.0 37.0 41.0 31.5 41.0 28-29 37.24599994054783 40.0 37.0 41.0 31.5 41.0 30-31 37.10572600430959 39.0 36.0 41.0 31.0 41.0 32-33 36.901046012522286 39.0 35.5 41.0 31.0 41.0 34-35 36.59691187460293 39.0 35.0 41.0 30.0 41.0 36-37 36.30292677497562 39.0 35.0 41.0 30.0 41.0 38-39 36.07678524148296 39.0 35.0 40.5 30.0 41.0 40-41 36.07560380245012 39.0 35.0 40.5 30.0 41.0 42-43 36.08027736510752 39.0 35.0 41.0 30.0 41.0 44-45 35.89756529541852 39.0 35.0 41.0 29.5 41.0 46-47 35.53654580132883 39.0 35.0 41.0 27.0 41.0 48-49 35.203420435369615 39.0 35.0 41.0 25.0 41.0 50-51 34.03138314088574 38.0 34.0 40.0 23.5 40.5 52-53 34.15666130444971 38.0 33.5 39.5 23.0 40.5 54-55 34.76647084320459 39.0 35.0 40.0 23.0 41.0 56-57 34.69702172285473 39.0 35.0 41.0 23.0 41.0 58-59 34.566849470564605 39.0 35.0 40.0 22.5 41.0 60-61 34.1769365679922 38.0 34.0 40.0 22.0 41.0 62-63 33.8310365900448 38.0 34.0 40.0 20.5 41.0 64-65 33.386441518076566 37.5 33.0 39.5 18.5 41.0 66-67 32.88742835495157 37.0 33.0 39.0 13.5 41.0 68-69 32.442914856889644 36.0 32.0 39.0 11.5 40.0 70-71 31.867901428303796 35.5 31.5 38.5 7.5 40.0 72-73 31.326435614337928 35.0 31.0 37.0 4.0 39.5 74-75 30.845656984489203 35.0 31.0 37.0 2.0 39.0 76-77 30.326576640413233 35.0 30.5 36.5 2.0 39.0 78-79 29.81263199591301 34.5 30.0 36.0 2.0 37.5 80-81 29.26882611692306 34.0 29.0 35.5 2.0 37.0 82-83 28.812954835016775 34.0 29.0 35.0 2.0 37.0 84-85 28.39215756760437 34.0 29.0 35.0 2.0 36.0 86-87 27.98889696178677 34.0 28.5 35.0 2.0 36.0 88-89 27.390650869902757 34.0 26.5 35.0 2.0 35.5 90-91 26.812319526373606 34.0 25.0 35.0 2.0 35.0 92-93 26.34522526495965 33.0 25.0 35.0 2.0 35.0 94-95 25.79931021657872 33.0 22.5 35.0 2.0 35.0 96-97 25.08201530409439 33.0 17.0 35.0 2.0 35.0 98-99 24.361636137517017 32.0 2.0 35.0 2.0 35.0 100-101 22.01907377668521 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17559947844116763 1101 2 0.2837314022482609 1101 3 0.36287075252199585 1101 4 0.2352823557928545 1101 5 0.23833730407689302 1101 6 0.28759040193855867 1101 7 0.3691113526821894 1101 8 0.4097623691258647 1101 9 0.4448151272652012 1101 10-11 0.41323885296282015 1101 12-13 0.3724595388801646 1101 14-15 0.291785093481046 1101 16-17 0.5116697617225867 1101 18-19 0.5701136370036153 1101 20-21 0.7417763871341876 1101 22-23 0.6672703548811469 1101 24-25 0.6416331747439514 1101 26-27 0.44703008396225385 1101 28-29 0.7072636640390186 1101 30-31 0.8746411110327443 1101 32-33 0.8139854113417968 1101 34-35 0.8363502454263667 1101 36-37 0.8305463049628017 1101 38-39 0.8372985537454909 1101 40-41 0.9996752160038227 1101 42-43 1.076343629008747 1101 44-45 1.073478925029299 1101 46-47 1.0174894909534373 1101 48-49 0.9869761396876839 1101 50-51 0.9823528764574689 1101 52-53 0.9712028087117375 1101 54-55 0.9484362548190433 1101 56-57 1.0282713604409182 1101 58-59 0.9180029898703097 1101 60-61 0.9538018305357312 1101 62-63 0.9357352043191867 1101 64-65 0.966250322769568 1101 66-67 0.8826316019493703 1101 68-69 0.9670767381207384 1101 70-71 0.8292168982111292 1101 72-73 0.7570715270681596 1101 74-75 0.8354686552675972 1101 76-77 0.8934001160791425 1101 78-79 0.6116240259703574 1101 80-81 0.5390811726798823 1101 82-83 0.7551850850306536 1101 84-85 0.7507366655232595 1101 86-87 0.4704376620825492 1101 88-89 0.5968896212348085 1101 90-91 0.2606195928062469 1101 92-93 0.1375372692240333 1101 94-95 0.1909224478305589 1101 96-97 0.1677652160392782 1101 98-99 0.45740418007830286 1101 100-101 0.8346467510857387 1102 1 0.21925963181221775 1102 2 0.22073406682691044 1102 3 0.18907572290784458 1102 4 0.4053062204053006 1102 5 0.3479209088421129 1102 6 0.30639862608406787 1102 7 0.2980074298362325 1102 8 0.3805541586722825 1102 9 0.6045935746641078 1102 10-11 0.420102736957908 1102 12-13 0.48775792141353236 1102 14-15 0.5845651657273905 1102 16-17 0.574246711994121 1102 18-19 0.5980834227569432 1102 20-21 0.6196823128533069 1102 22-23 0.5127445824902921 1102 24-25 0.557056603139948 1102 26-27 0.5435477866697269 1102 28-29 0.5768509378145836 1102 30-31 0.733612857513755 1102 32-33 0.7060598141649024 1102 34-35 0.5505629538940795 1102 36-37 0.7594592296133058 1102 38-39 0.6622327415410538 1102 40-41 0.7603864798655735 1102 42-43 0.9426533961709964 1102 44-45 0.9107858607424575 1102 46-47 0.886534703215851 1102 48-49 0.9123042914798845 1102 50-51 0.8203013541390902 1102 52-53 0.6743453749010015 1102 54-55 0.6774865266787984 1102 56-57 0.8286459097035319 1102 58-59 0.879852502683498 1102 60-61 0.8796514524565211 1102 62-63 0.8333334466172104 1102 64-65 0.6976734174246815 1102 66-67 0.6260509163471824 1102 68-69 0.7722442664165072 1102 70-71 0.7765462758592605 1102 72-73 0.5205158292448608 1102 74-75 0.5017103906346136 1102 76-77 0.5853829540960795 1102 78-79 0.4201380553887617 1102 80-81 0.42829765177775414 1102 82-83 0.537809325957344 1102 84-85 0.5978742491323032 1102 86-87 0.4647814494211495 1102 88-89 0.6224496801529114 1102 90-91 0.5249391625372333 1102 92-93 0.34723297669587083 1102 94-95 0.28595810569052915 1102 96-97 0.2864745480547626 1102 98-99 0.18238029942061829 1102 100-101 0.3643716180974863 1103 1 0.06547203707973992 1103 2 0.16267882373229625 1103 3 0.2528234362618562 1103 4 0.25660889847963375 1103 5 0.1869410313268034 1103 6 0.10481018827515953 1103 7 0.18339675617044549 1103 8 0.1165246623593248 1103 9 0.2580005351576844 1103 10-11 0.25331392646831574 1103 12-13 0.309865385253282 1103 14-15 0.31893927805425903 1103 16-17 0.339565819832913 1103 18-19 0.44042983400099445 1103 20-21 0.48461965023158626 1103 22-23 0.41978461157756186 1103 24-25 0.38743576029076365 1103 26-27 0.4012104651985524 1103 28-29 0.4288950004885663 1103 30-31 0.487163550040826 1103 32-33 0.3614892491218953 1103 34-35 0.43750606327812847 1103 36-37 0.5143904313949079 1103 38-39 0.46511533576222774 1103 40-41 0.5200102768909431 1103 42-43 0.7100830356478767 1103 44-45 0.6738069603777248 1103 46-47 0.6292316388261696 1103 48-49 0.59229315650812 1103 50-51 0.7638254590805076 1103 52-53 0.6121741690290037 1103 54-55 0.7083321027999645 1103 56-57 0.7188499751044546 1103 58-59 0.5988620583072262 1103 60-61 0.43147452002761355 1103 62-63 0.6065436135281601 1103 64-65 0.7306124818598079 1103 66-67 0.5904843394420283 1103 68-69 0.608338618258891 1103 70-71 0.6601176476196144 1103 72-73 0.8768150418432832 1103 74-75 0.6096997328759208 1103 76-77 0.6872679457448498 1103 78-79 0.615431825485679 1103 80-81 0.641313638415788 1103 82-83 0.8032789831592844 1103 84-85 0.6762792212380617 1103 86-87 0.7001810017498364 1103 88-89 0.6629957482580338 1103 90-91 0.6952701438918361 1103 92-93 0.49686200034064143 1103 94-95 0.5415199104649311 1103 96-97 0.6107607168291231 1103 98-99 0.8197097241516182 1103 100-101 1.1954495515376244 1104 1 0.030107211727592897 1104 2 0.13630319637908883 1104 3 0.129668087229021 1104 4 0.2857052469907657 1104 5 0.25838144642129635 1104 6 0.164605391070495 1104 7 0.16566707715689688 1104 8 0.12885435188773187 1104 9 0.27256261127720904 1104 10-11 0.18495770308405923 1104 12-13 0.2202003847881997 1104 14-15 0.4152417866582496 1104 16-17 0.5616968795290376 1104 18-19 0.3674554114085993 1104 20-21 0.3381288851134414 1104 22-23 0.2843192883870316 1104 24-25 0.1934645689970651 1104 26-27 0.37143317811443666 1104 28-29 0.298901956475774 1104 30-31 0.37355953983332313 1104 32-33 0.3196029330046315 1104 34-35 0.304240773249866 1104 36-37 0.3008797911609875 1104 38-39 0.5196935481544003 1104 40-41 0.3837065449229229 1104 42-43 0.3009916210346404 1104 44-45 0.47031425607593746 1104 46-47 0.6192278575191779 1104 48-49 0.5992799805742095 1104 50-51 0.7686574942325137 1104 52-53 0.7096499949513841 1104 54-55 0.6009417202611118 1104 56-57 0.5485966275356091 1104 58-59 0.7250111105539716 1104 60-61 0.6306083580744328 1104 62-63 0.6995210490336206 1104 64-65 0.75686552004899 1104 66-67 0.5483611861150877 1104 68-69 0.656599318144444 1104 70-71 0.49794305234078706 1104 72-73 0.49669713381599223 1104 74-75 0.4770463776231395 1104 76-77 0.6564538323703459 1104 78-79 0.571316914194739 1104 80-81 0.31796765691386497 1104 82-83 0.2048826010814615 1104 84-85 0.25673739634290627 1104 86-87 0.3654359973214589 1104 88-89 0.5672869756257981 1104 90-91 0.5657757976535294 1104 92-93 0.5062245265772489 1104 94-95 0.4323753654552469 1104 96-97 0.24669112055947195 1104 98-99 0.7205785653731205 1104 100-101 0.8874183688984729 1105 1 0.09531696919552246 1105 2 0.15171116216864533 1105 3 0.10340548461329746 1105 4 0.03656753122462675 1105 5 0.22580192898833218 1105 6 0.1648629058190636 1105 7 0.18538256237711437 1105 8 0.1870175694174634 1105 9 0.13618514147666616 1105 10-11 0.1460729760497017 1105 12-13 0.0273432976701784 1105 14-15 0.3002595005515687 1105 16-17 0.2768992236935901 1105 18-19 0.25077001639658647 1105 20-21 0.2920317942497448 1105 22-23 0.1464942735604211 1105 24-25 0.1423127757580076 1105 26-27 0.14519866801673942 1105 28-29 0.22925202046428694 1105 30-31 0.2554636663120817 1105 32-33 0.30640920387892834 1105 34-35 0.09238807168604524 1105 36-37 0.4470603792310399 1105 38-39 0.38620141405944963 1105 40-41 0.11919207727613923 1105 42-43 0.015333631834657524 1105 44-45 0.1051485882543588 1105 46-47 0.17975747951874155 1105 48-49 0.18732661415413787 1105 50-51 0.2357268203242171 1105 52-53 0.33303467516488894 1105 54-55 0.28717755694994196 1105 56-57 0.15173323520799187 1105 58-59 0.1886039875399561 1105 60-61 0.13248314347881518 1105 62-63 -0.03288796242188141 1105 64-65 -0.060991567069031305 1105 66-67 0.08561498640849408 1105 68-69 0.1541802402033099 1105 70-71 -0.12732421675432448 1105 72-73 -0.07764464287970085 1105 74-75 -0.10326896089170035 1105 76-77 1.0336260101695416E-4 1105 78-79 -0.007870821860450405 1105 80-81 0.0993590949386487 1105 82-83 0.10375538879566903 1105 84-85 0.07749932785959146 1105 86-87 -0.0950881622440427 1105 88-89 0.04013282573660604 1105 90-91 0.08951397538552541 1105 92-93 0.22734775908966043 1105 94-95 0.33117597100508434 1105 96-97 0.1819242104243166 1105 98-99 0.33061929952425473 1105 100-101 0.6451340570090949 1106 1 0.1541363492976302 1106 2 0.2863906324834744 1106 3 0.22430377987503292 1106 4 0.2524108066403201 1106 5 0.2082007256436711 1106 6 0.20105952074496258 1106 7 0.19812663430326438 1106 8 0.19563038679198996 1106 9 0.09201436643272132 1106 10-11 0.20065672403364232 1106 12-13 0.21280340732858605 1106 14-15 0.2639992582049686 1106 16-17 0.31902367416239485 1106 18-19 0.2876655797304153 1106 20-21 0.3717683910263432 1106 22-23 0.3253426124796235 1106 24-25 0.22287629069877113 1106 26-27 0.4150108646545476 1106 28-29 0.33726564388145874 1106 30-31 0.2062115148950241 1106 32-33 0.3090148301633917 1106 34-35 0.23371434356720755 1106 36-37 0.4944959320413176 1106 38-39 0.4072606604820166 1106 40-41 0.5728119770736768 1106 42-43 0.5432086380305847 1106 44-45 0.6054812619595964 1106 46-47 0.647843442785458 1106 48-49 0.4530847670429665 1106 50-51 0.5012411872528304 1106 52-53 0.47864614659307847 1106 54-55 0.26539982111383864 1106 56-57 0.5762618203629231 1106 58-59 0.5503933145286553 1106 60-61 0.6650520422049269 1106 62-63 0.6114876595540295 1106 64-65 0.5267718552187759 1106 66-67 0.6370459599509246 1106 68-69 0.625810989548448 1106 70-71 0.6996280250279625 1106 72-73 0.4573845161135459 1106 74-75 0.5025103153475783 1106 76-77 0.6513845853151032 1106 78-79 0.6054696320246862 1106 80-81 0.6194796581771556 1106 82-83 0.5196306447351837 1106 84-85 0.6151588968517316 1106 86-87 0.7359245575207787 1106 88-89 0.8435112273014127 1106 90-91 0.8470108106958385 1106 92-93 1.0965271607726628 1106 94-95 1.1540646368405412 1106 96-97 1.0240521878063156 1106 98-99 0.8470706815023021 1106 100-101 1.1343383663251032 1107 1 -0.03493646448914234 1107 2 -0.015576406325671144 1107 3 0.028029167124444143 1107 4 0.1530088102131515 1107 5 0.021726635279421203 1107 6 -0.12650180681636414 1107 7 -0.04702180992984495 1107 8 -0.09309207813184628 1107 9 -0.010125040054511203 1107 10-11 -0.09119444539189914 1107 12-13 -0.045469085497686024 1107 14-15 -0.0015972099086667413 1107 16-17 -0.005346209704057969 1107 18-19 0.0063578968209014874 1107 20-21 -0.04489579909322572 1107 22-23 -0.0016474582175405317 1107 24-25 0.007007237301117186 1107 26-27 -0.07669600462669024 1107 28-29 -0.02250730628050235 1107 30-31 -0.23428036037946498 1107 32-33 -0.2932946325579664 1107 34-35 -0.12891984400977208 1107 36-37 -0.13085367385748725 1107 38-39 -0.5080414287331152 1107 40-41 -0.5565232186230702 1107 42-43 -0.5066326978519413 1107 44-45 -0.08300543568041263 1107 46-47 -0.21234886935193487 1107 48-49 -0.3005384213628304 1107 50-51 -0.17578735247593613 1107 52-53 -0.2859910722775538 1107 54-55 -0.21407950962407796 1107 56-57 -0.12090600037468846 1107 58-59 -0.20750604520136307 1107 60-61 -0.27839517927432667 1107 62-63 -0.22769032493287966 1107 64-65 -0.3237817356094155 1107 66-67 -0.4804463875185405 1107 68-69 -0.09845520954612397 1107 70-71 -0.19854296238786873 1107 72-73 -0.11738574286723846 1107 74-75 -0.2894531242131144 1107 76-77 -0.2992149041859875 1107 78-79 -0.1288860199511035 1107 80-81 -0.31190512789870795 1107 82-83 -0.4355141342082227 1107 84-85 -0.229383288651551 1107 86-87 -0.3807604355944214 1107 88-89 -0.31705818040758516 1107 90-91 -0.145145139126015 1107 92-93 -0.025137343042871407 1107 94-95 -0.018458238199496435 1107 96-97 0.054208276314806625 1107 98-99 0.27325141660752195 1107 100-101 0.6255548792807453 1108 1 0.040874396975368654 1108 2 0.26853930818053584 1108 3 0.12374499628679558 1108 4 0.1593093856987906 1108 5 0.18206883976424137 1108 6 0.0917637985460118 1108 7 0.07383017340091413 1108 8 0.07168202721950934 1108 9 0.2441690529492604 1108 10-11 0.21776251445640327 1108 12-13 0.1589688789148198 1108 14-15 0.2954586629112157 1108 16-17 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-1.4483084829085406 2316 72-73 -1.296915000794666 2316 74-75 -1.4282281249560782 2316 76-77 -1.4536947797642625 2316 78-79 -1.2650893741864948 2316 80-81 -1.1169592652182914 2316 82-83 -1.0831436823869716 2316 84-85 -1.060741724266279 2316 86-87 -1.0350485273653511 2316 88-89 -1.1413749010629743 2316 90-91 -1.4346731092489513 2316 92-93 -1.3980760138305115 2316 94-95 -1.4130023058785746 2316 96-97 -1.5784046789983783 2316 98-99 -1.9249514026936936 2316 100-101 -2.221879715110372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8367.0 3 1549.0 4 1138.0 5 1220.0 6 1365.0 7 1693.0 8 2178.0 9 2448.0 10 3175.0 11 4365.0 12 6348.0 13 8155.0 14 9233.0 15 10636.0 16 13179.0 17 16704.0 18 14697.0 19 9793.0 20 10037.0 21 10318.0 22 11533.0 23 12896.0 24 14709.0 25 17182.0 26 19690.0 27 22922.0 28 27195.0 29 32033.0 30 38263.0 31 45575.0 32 55531.0 33 69300.0 34 89753.0 35 131215.0 36 177057.0 37 266681.0 38 243240.0 39 35168.0 >>END_MODULE >>Per base sequence content fail #Base G 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19.74692468711341 18-19 16.352863616664308 33.97778753173742 32.2203079980198 17.449040853578477 20-21 19.333754686106612 29.5237963307677 29.30007532402317 21.842373659102517 22-23 17.98940955662263 28.90942270892357 30.242461767030342 22.858705967423457 24-25 21.337101123027203 29.388932827958968 30.24368342667932 19.030282622334514 26-27 21.038937887976882 26.174668887665792 34.78995362051643 17.9964396038409 28-29 17.409227908047523 30.757093143436254 33.540991370657466 18.292687577858764 30-31 18.142267773939437 30.128086474315115 31.651071812587222 20.078573939158233 32-33 18.44629767122301 30.472771372055785 31.711302020711322 19.36962893600988 34-35 20.919583529317087 27.654660667135637 31.126729686717365 20.299026116829904 36-37 20.56933902831982 26.879801848337497 34.122726750647935 18.42813237269474 38-39 20.322404041707692 28.232661866410208 33.54087260863199 17.904061483250103 40-41 17.915914953593205 29.7739069034138 34.10450250987198 18.205675633121015 42-43 16.84066023656002 31.079257389985436 30.509817580820975 21.57026479263357 44-45 18.538623940268163 29.204899703873583 30.24264324486549 22.01383311099276 46-47 20.453111825875155 29.91145253238124 29.618364578237173 20.01707106350644 48-49 19.849136539899067 28.802212423469904 30.92287959310731 20.42577144352372 50-51 17.855073187963736 29.86194538195088 33.48823877646909 18.7947426536163 52-53 18.249611348620288 31.51646910221531 30.808915385042475 19.425004164121926 54-55 16.417452109200774 28.455776546268964 32.65675915474435 22.47001218978591 56-57 19.878931021072415 29.48562856614544 29.444657030781386 21.190783382000763 58-59 21.148264787286436 27.933109509506732 30.001213920547166 20.917411782659663 60-61 19.10840481059479 28.261654971648337 32.8754835940355 19.754456623721367 62-63 19.79352607517397 28.179395559311814 31.96682095312044 20.060257412393778 64-65 18.781355389248468 30.427729591296654 31.57277504500599 19.218139974448885 66-67 18.397836422489043 28.5265903870463 30.159686014701055 22.9158871757636 68-69 17.053709554871467 29.786087143049038 31.229007095718792 21.931196206360703 70-71 20.215685253640167 29.15912601308551 29.203068325784002 21.422120407490326 72-73 19.135925655857783 26.606747016887844 31.946171575846066 22.311155751408304 74-75 17.890084170217285 31.14953965453875 30.007048600639337 20.95332757460463 76-77 19.689337259992666 27.187049658221714 30.651989927265195 22.47162315452043 78-79 18.889689878322844 27.5297948410884 32.29568582773701 21.28482945285175 80-81 20.191398615737597 28.727417437098772 30.35163068387031 20.729553263293315 82-83 19.097305232871836 27.664813897882475 31.775406810495376 21.462474058750317 84-85 18.79505614234757 27.634734694444056 30.95847964579661 22.61172951741176 86-87 19.512522626706957 28.351068646748573 30.928249267982444 21.20815945856203 88-89 20.231038367942357 26.953435265222897 30.03380803849182 22.78171832834292 90-91 19.573982339154455 25.550127043833506 31.95482993896135 22.921060678050694 92-93 20.80268296771547 28.202466467241894 31.01069070870066 19.984159856341975 94-95 19.989774607497303 27.310058822038947 30.67992612448947 22.02024044597428 96-97 18.348446483827914 26.525826566402124 31.56174235390284 23.563984595867122 98-99 20.40569752234554 27.466721182654474 29.140658190309853 22.98692310469013 100-101 20.794552157883324 25.354118412491864 30.862337887659947 22.988991541964868 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9279.0 1 5645.0 2 2217.0 3 2267.0 4 1796.0 5 1808.5 6 3035.0 7 5153.5 8 7053.0 9 7257.0 10 5644.0 11 4112.5 12 3752.5 13 3538.0 14 3207.0 15 2980.0 16 2960.5 17 3034.0 18 2953.0 19 3095.5 20 3426.0 21 3807.5 22 4326.0 23 4974.5 24 5537.0 25 6672.5 26 9552.5 27 12514.5 28 13146.0 29 14224.5 30 17631.5 31 21280.0 32 26949.5 33 34473.0 34 41750.0 35 46955.0 36 54441.5 37 61956.5 38 59448.0 39 53556.5 40 48736.5 41 43033.5 42 37222.5 43 35287.5 44 35843.0 45 35773.5 46 45304.5 47 81086.0 48 177416.5 49 175632.0 50 71632.5 51 28101.0 52 13857.0 53 8833.5 54 6189.5 55 4748.5 56 3921.5 57 3396.0 58 2955.5 59 2573.5 60 2262.5 61 1936.0 62 1615.5 63 1257.5 64 959.5 65 771.5 66 648.5 67 543.5 68 478.5 69 386.0 70 273.0 71 211.5 72 168.0 73 154.0 74 140.5 75 101.5 76 81.5 77 61.5 78 38.5 79 30.0 80 23.0 81 22.0 82 18.0 83 12.0 84 9.0 85 5.5 86 4.0 87 3.5 88 2.0 89 1.5 90 2.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26912476037665023 2 0.16058998673387065 3 0.18734346278467048 4 0.15810129128728462 5 0.2287525897986991 6 0.18838041922074797 7 0.23345345897558384 8 0.2397443280211207 9 0.14551955319621082 10-11 0.1997869400176006 12-13 0.20621606992128116 14-15 0.16743389921198223 16-17 0.2796671508101049 18-19 0.22484672055614047 20-21 0.1920788971760911 22-23 0.23832715422514814 24-25 0.22498498141428416 26-27 0.3351097549257159 28-29 0.3060058442864737 30-31 0.2819138897549396 32-33 0.1861682454904493 34-35 0.3522195361209948 36-37 0.388824098314531 38-39 0.37109214325760553 40-41 0.3341764941332461 42-43 0.3663912740807208 44-45 0.3756201863618107 46-47 0.38063214246951865 48-49 0.3987443148863392 50-51 0.3914164894047248 52-53 0.391347358975653 54-55 0.3857823594353703 56-57 0.3661493175789694 58-59 0.34088214575321407 60-61 0.30928953966738587 62-63 0.3726475779117218 64-65 0.40783496630928545 66-67 0.4122938789844187 68-69 0.4164762699432646 70-71 0.41588866129615404 72-73 0.40714366201856705 74-75 0.40299583627425695 76-77 0.38861670702731554 78-79 0.3641791003504221 80-81 0.37416844735130217 82-83 0.3832936639887843 84-85 0.3844343160684695 86-87 0.37949149038983343 88-89 0.3879254027365972 90-91 0.40762757502206987 92-93 0.40769670545114167 94-95 0.41543931350718716 96-97 0.4160614873688337 98-99 0.4137456179949272 100-101 0.3873723593040225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1446541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.45112047947278 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52285964678872 13.703118840888418 2 8.617754857239708 3.007789565525038 3 3.1162326375566307 1.6314525360019803 4 1.680806288172529 1.1732781215021697 5 1.1111633635834308 0.9695522865135334 6 0.7853305882584714 0.822293922714829 7 0.5943727465318943 0.7260729286610258 8 0.4521042112012748 0.6311780047160365 9 0.3855004265560506 0.6054672949846008 >10 3.4073058288012525 12.592642395346601 >50 0.6614947292004937 8.15953313896508 >100 0.5902935522407772 20.305260526355315 >500 0.04256159709552774 4.970785974940868 >1k 0.020286368709083312 7.338207048763408 >5k 0.0075576667739722155 9.792258865882548 >10k+ 0.00437549129019444 13.57110854823855 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38404 2.6548849980747176 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25902 1.7906163738186474 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22838 1.5788007391425476 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18735 1.2951585886608121 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18222 1.2596946785469614 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14477 1.0008012216729427 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14186 0.9806842668130388 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12151 0.8400038436518564 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10290 0.7113521151491731 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10248 0.708448637128156 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10052 0.6948990730300766 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9400 0.6498260332752408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9348 0.6462312509635053 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9096 0.6288103828374031 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8944 0.6183025576184844 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8928 0.617196470753335 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8886 0.614292992732318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8465 0.5851890820930759 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8150 0.5634129969354481 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7995 0.5526977804293138 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6992 0.48335996007026416 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6801 0.47015604811754386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6600 0.4562608318741052 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6513 0.4502464845448556 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6391 0.4418125721980919 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6258 0.43261822513153797 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5729 0.39604822815253765 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5576 0.38547127250454705 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5520 0.38159996847652433 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5475 0.3784890991682918 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4728 0.3268486686516317 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4717 0.32608823393184155 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4589 0.3172395390106468 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4116 0.28454084605966923 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4110 0.2841260634852382 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3931 0.27175171668137993 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3714 0.25675041357279194 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3497 0.24174911046420394 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3490 0.24126519746070105 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3489 0.24119606703162924 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3342 0.2310338939580696 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3134 0.21665476471112813 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2617 0.18091433288098988 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2549 0.17621346370410518 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2478 0.17130520324000495 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2472 0.17089042066557394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2457 0.16985346422949643 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2031 0.14040390144489512 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2030 0.14033477101582326 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1921 0.13279955424699333 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.12408912018394223 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1745 0.12063259873035052 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1701 0.11759085985118983 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1656 0.11447999054295731 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1558 0.10770520849391754 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1514 0.10466346961475685 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1449 0.10016999172508764 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0369564360775118E-4 0.0 12-13 0.0 0.0 0.0 1.3826085814366823E-4 0.0 14-15 0.0 0.0 0.0 2.4195650175141942E-4 0.0 16-17 0.0 0.0 0.0 5.530434325746729E-4 0.0 18-19 0.0 0.0 0.0 9.332607924697606E-4 0.0 20-21 0.0 0.0 0.0 0.001624565083188102 0.0 22-23 0.0 0.0 0.0 0.003663912740807208 0.0 24-25 0.0 0.0 0.0 0.007189564623470748 0.0 26-27 0.0 0.0 0.0 0.01527782482487534 0.0 28-29 3.456521453591706E-5 0.0 0.0 0.04158195308670823 0.0 30-31 6.913042907183412E-5 0.0 0.0 0.10186368723734757 0.0 32-33 6.913042907183412E-5 0.0 0.0 0.22861432894055544 0.0 34-35 6.913042907183412E-5 0.0 0.0 0.4460640935860097 0.0 36-37 6.913042907183412E-5 0.0 0.0 0.7508255901491904 0.0 38-39 6.913042907183412E-5 0.0 0.0 1.1304207761826315 0.0 40-41 6.913042907183412E-5 0.0 0.0 1.526572699978777 0.0 42-43 6.913042907183412E-5 0.0 0.0 1.93789875295619 0.0 44-45 6.913042907183412E-5 0.0 0.0 2.4045291491910703 0.0 46-47 6.913042907183412E-5 0.0 0.0 2.9861580141869464 0.0 48-49 6.913042907183412E-5 0.0 0.0 3.550089489340434 0.0 50-51 6.913042907183412E-5 0.0 0.0 4.113053138486915 0.0 52-53 6.913042907183412E-5 0.0 0.0 4.618258314143878 0.0 54-55 6.913042907183412E-5 0.0 0.0 5.166670007970739 0.0 56-57 6.913042907183412E-5 0.0 0.0 5.880199731635674 0.0 58-59 6.913042907183412E-5 0.0 0.0 6.604617497879424 0.0 60-61 6.913042907183412E-5 0.0 0.0 7.470614382862291 0.0 62-63 6.913042907183412E-5 0.0 0.0 8.246361492691875 0.0 64-65 6.913042907183412E-5 0.0 0.0 8.950143825857683 0.0 66-67 6.913042907183412E-5 0.0 0.0 9.764085497749459 0.0 68-69 6.913042907183412E-5 0.0 0.0 10.675812161563343 0.0 70-71 6.913042907183412E-5 0.0 0.0 11.645228168437672 0.0 72-73 6.913042907183412E-5 0.0 0.0 12.581461569357522 0.0 74-75 6.913042907183412E-5 0.0 0.0 13.61668974470824 0.0 76-77 6.913042907183412E-5 0.0 0.0 14.529280538885521 0.0 78-79 6.913042907183412E-5 0.0 0.0 15.731942613448219 0.0 80-81 6.913042907183412E-5 6.913042907183412E-5 0.0 16.953719251649282 0.0 82-83 6.913042907183412E-5 6.913042907183412E-5 0.0 18.00235873023993 0.0 84-85 6.913042907183412E-5 6.913042907183412E-5 0.0 19.27155884278427 0.0 86-87 6.913042907183412E-5 6.913042907183412E-5 0.0 20.576291995871532 0.0 88-89 6.913042907183412E-5 1.3826085814366823E-4 0.0 21.778642983503406 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGGT 20 0.0020851584 71.15527 4 CTTATGT 245 0.0 34.851566 2 GCAACGC 70 2.0697011E-4 33.883465 9 GCTTATG 285 0.0 33.34681 1 GTATGGT 105 1.8961255E-6 31.679468 1 GTAAGCG 150 0.0 31.673971 94-95 GGCTCGC 30 0.0039474736 31.673967 90-91 TTAGACG 90 2.435431E-5 31.624567 3 GACATAT 335 0.0 31.206638 1 CATTCCG 310 0.0 30.604418 9 CCTTAGA 110 2.7254846E-6 30.239492 1 GCTTTGC 520 0.0 30.156418 1 TTAGGCA 350 0.0 29.817446 4 TAGGCAT 320 0.0 29.648031 5 CCTTTAG 310 0.0 29.12467 1 CATATAG 375 0.0 27.829618 3 TAGACGC 105 6.969869E-5 27.106771 4 CTTAGAC 105 6.969869E-5 27.106771 2 CTTTGCG 570 0.0 26.631214 2 TCAGGCC 125 7.469389E-6 26.564638 3 >>END_MODULE