##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1446541 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937873174697433 31.0 31.0 34.0 30.0 34.0 2 32.10996784743744 33.0 31.0 34.0 30.0 34.0 3 32.183230893559184 34.0 31.0 34.0 30.0 34.0 4 35.779521631256905 37.0 35.0 37.0 35.0 37.0 5 35.69463430348673 37.0 35.0 37.0 33.0 37.0 6 35.78346621353975 37.0 35.0 37.0 33.0 37.0 7 35.7262013313138 37.0 35.0 37.0 33.0 37.0 8 35.75548221585147 37.0 35.0 37.0 33.0 37.0 9 37.315521647848215 39.0 37.0 39.0 34.0 39.0 10-11 37.239831432361754 39.0 37.0 39.0 33.5 39.0 12-13 37.20782750022295 39.0 37.0 39.0 33.0 39.0 14-15 38.19374148399527 40.0 37.0 41.0 33.0 41.0 16-17 38.18110824373454 40.0 37.5 41.0 33.0 41.0 18-19 38.45686641443278 40.0 38.0 41.0 34.0 41.0 20-21 38.476302088914174 40.0 38.0 41.0 34.0 41.0 22-23 38.41052068347872 40.0 38.0 41.0 34.0 41.0 24-25 38.27714319884469 40.0 37.5 41.0 33.5 41.0 26-27 37.98926992045162 40.0 37.0 41.0 33.0 41.0 28-29 37.84395948680334 40.0 37.0 41.0 33.0 41.0 30-31 37.70199842244361 40.0 37.0 41.0 33.0 41.0 32-33 37.508811364489496 40.0 36.0 41.0 32.5 41.0 34-35 37.4096029770328 40.0 36.0 41.0 32.0 41.0 36-37 37.215981434331965 39.5 35.5 41.0 31.5 41.0 38-39 37.024755260998475 39.0 35.0 41.0 31.0 41.0 40-41 36.93764055080361 39.0 35.0 41.0 31.0 41.0 42-43 36.76850362347144 39.0 35.0 41.0 31.0 41.0 44-45 36.53910950329096 39.0 35.0 41.0 30.0 41.0 46-47 36.193275199251175 39.0 35.0 41.0 30.0 41.0 48-49 35.849490612433385 39.0 35.0 41.0 28.0 41.0 50-51 35.65236588523934 39.0 35.0 41.0 28.0 41.0 52-53 35.459462607696565 39.0 35.0 40.0 27.0 41.0 54-55 35.28292181141081 39.0 35.0 40.0 27.0 41.0 56-57 35.037341146915296 38.5 34.5 40.0 26.0 41.0 58-59 34.79616097988236 38.0 34.0 40.0 26.0 41.0 60-61 34.315622232622516 38.0 34.0 40.0 23.5 41.0 62-63 33.9925145571401 37.5 33.5 40.0 23.0 41.0 64-65 33.52379020020864 37.0 33.0 39.5 22.0 41.0 66-67 33.03322996029839 36.5 32.0 39.0 21.5 40.0 68-69 32.665867058037065 36.0 32.0 39.0 20.5 40.0 70-71 32.1209682269635 35.5 31.5 37.5 19.0 40.0 72-73 31.557222712664213 35.0 31.0 37.0 16.0 39.0 74-75 31.06918435080651 35.0 31.0 37.0 12.5 39.0 76-77 29.059246851627435 32.5 28.5 34.5 7.5 36.5 78-79 29.98707019019855 34.0 30.0 36.0 7.0 37.0 80-81 29.998177721889668 34.0 30.0 35.0 2.0 37.0 82-83 29.664250442953225 34.0 30.0 35.0 2.0 36.5 84-85 29.288975563084627 34.0 29.5 35.0 2.0 36.0 86-87 28.80862035711397 34.0 29.0 35.0 2.0 36.0 88-89 28.148030716032245 34.0 28.0 35.0 2.0 35.0 90-91 27.571745287551476 33.0 26.5 35.0 2.0 35.0 92-93 27.344457225892665 33.0 26.0 35.0 2.0 35.0 94-95 27.036456277423177 33.0 26.0 35.0 2.0 35.0 96-97 26.406359722952892 33.0 25.0 35.0 2.0 35.0 98-99 25.657918441302392 33.0 23.5 34.0 2.0 35.0 100-101 22.18600578898213 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09421872730166214 1101 2 0.14744776969475737 1101 3 0.11506914638410848 1101 4 0.0961128751657725 1101 5 0.0038663701272128037 1101 6 0.07420998742321672 1101 7 0.17778566964312859 1101 8 0.2360722108590636 1101 9 0.2311213967125667 1101 10-11 0.20376830464593354 1101 12-13 0.2746501860393309 1101 14-15 0.2716989306877551 1101 16-17 0.38381191051929875 1101 18-19 0.4139001711323047 1101 20-21 0.3518493046650235 1101 22-23 0.1597190886921993 1101 24-25 0.24335843595360984 1101 26-27 0.18729414881220663 1101 28-29 0.32718017238210706 1101 30-31 0.28014341993136327 1101 32-33 0.31855667382321684 1101 34-35 0.4500733356425144 1101 36-37 0.35431832232963956 1101 38-39 0.361106105576809 1101 40-41 0.407578809256286 1101 42-43 0.3958183829946549 1101 44-45 0.3436199766674264 1101 46-47 0.3898748351849477 1101 48-49 0.2374601703717758 1101 50-51 0.3662098944015 1101 52-53 0.3594586948922611 1101 54-55 0.48997249443936397 1101 56-57 0.42797579734922664 1101 58-59 0.3589136106554278 1101 60-61 0.29732706646514373 1101 62-63 0.19586987741647732 1101 64-65 0.45994087346240775 1101 66-67 0.4620964650713333 1101 68-69 0.5033230496886318 1101 70-71 0.4922390745372667 1101 72-73 0.2707335179437784 1101 74-75 0.40240958136960714 1101 76-77 0.3747918421380092 1101 78-79 0.25693804785477425 1101 80-81 0.33848693330179813 1101 82-83 0.46659236264348536 1101 84-85 0.4866782243032475 1101 86-87 0.5951128607000236 1101 88-89 0.4204805309408286 1101 90-91 0.4472146537971078 1101 92-93 0.3597201423416969 1101 94-95 0.6446279961141208 1101 96-97 0.3871805701221547 1101 98-99 0.20499233797675842 1101 100-101 1.0345970250668408 1102 1 0.2855731269585071 1102 2 0.26133791546036633 1102 3 0.21051529483248288 1102 4 0.16255351795926742 1102 5 0.10393622038238703 1102 6 0.08551064624055726 1102 7 0.2434149584006633 1102 8 0.29240863450461063 1102 9 0.35002353896533833 1102 10-11 0.3809569014045877 1102 12-13 0.38994188305823485 1102 14-15 0.39032643200614103 1102 16-17 0.3233411647260951 1102 18-19 0.3695851047638641 1102 20-21 0.3237201362467559 1102 22-23 0.34469711206903497 1102 24-25 0.34682827144717265 1102 26-27 0.36358127079984115 1102 28-29 0.46045429879930566 1102 30-31 0.4084716027443491 1102 32-33 0.4872046595579391 1102 34-35 0.580979452834768 1102 36-37 0.4853466507263917 1102 38-39 0.5230359515318597 1102 40-41 0.4808988464384427 1102 42-43 0.34336998714374545 1102 44-45 0.31536057442730225 1102 46-47 0.4009064689000539 1102 48-49 0.5079108917120436 1102 50-51 0.6107232263870372 1102 52-53 0.6084253817759233 1102 54-55 0.5203062781089542 1102 56-57 0.5716632811034259 1102 58-59 0.6222306206366284 1102 60-61 0.5890460138549614 1102 62-63 0.7041904013040821 1102 64-65 0.6164867409052377 1102 66-67 0.6782003718720588 1102 68-69 0.5813528981039511 1102 70-71 0.6705150033691609 1102 72-73 0.3475332316967972 1102 74-75 0.16748728844138583 1102 76-77 0.37770913323172906 1102 78-79 0.4141487228564067 1102 80-81 0.6238407360759091 1102 82-83 0.5697431653865799 1102 84-85 0.6905527206165836 1102 86-87 0.7951931938418859 1102 88-89 0.7947885801932806 1102 90-91 0.8821250197675177 1102 92-93 0.775100248603934 1102 94-95 0.4874729445928736 1102 96-97 0.6784144165820933 1102 98-99 0.8050116457425425 1102 100-101 1.4435413876805896 1103 1 0.14982873137158847 1103 2 0.11583337625503987 1103 3 0.045496149498376326 1103 4 0.011146307496943564 1103 5 -0.16443932358370716 1103 6 -0.08609858442379448 1103 7 0.04739400647419956 1103 8 -0.04731594537745565 1103 9 0.07685882091872998 1103 10-11 0.07556893853629987 1103 12-13 0.08226159309447922 1103 14-15 0.0976783539249908 1103 16-17 0.08535386768367204 1103 18-19 -0.10246100310902051 1103 20-21 0.10213703867940183 1103 22-23 0.18248443410832493 1103 24-25 0.2332913272015631 1103 26-27 0.1569110818461894 1103 28-29 -0.04178689832377813 1103 30-31 0.18767533864099306 1103 32-33 0.2741432240555568 1103 34-35 0.2146445628778224 1103 36-37 0.24905516443489972 1103 38-39 0.17306549329368437 1103 40-41 0.2905391186771382 1103 42-43 0.3797262380669224 1103 44-45 0.37821067271047326 1103 46-47 0.32082556992151723 1103 48-49 0.412914243565794 1103 50-51 0.3450348465254294 1103 52-53 0.37035570866688516 1103 54-55 0.2242495352018139 1103 56-57 0.11208152427757767 1103 58-59 0.22377758635682454 1103 60-61 0.2047247358328974 1103 62-63 0.23464114041623674 1103 64-65 0.25698054281636473 1103 66-67 0.42585975535821063 1103 68-69 0.12963947591808278 1103 70-71 0.0875367392885309 1103 72-73 0.18989100515693025 1103 74-75 0.3158068166821728 1103 76-77 0.3702469978168388 1103 78-79 0.24311328410443167 1103 80-81 0.42397935511217 1103 82-83 0.4078741944624902 1103 84-85 0.36736584624861024 1103 86-87 0.40731554218470833 1103 88-89 0.15014188830249253 1103 90-91 0.11312455391665921 1103 92-93 0.23857072388543443 1103 94-95 0.3105973267723705 1103 96-97 0.4418189910102903 1103 98-99 0.5761851354199727 1103 100-101 1.2685007677580806 1104 1 0.07498968968015518 1104 2 0.1542439736206589 1104 3 0.09656098172562366 1104 4 0.06532212411770644 1104 5 -0.05519443239233368 1104 6 0.0900597694337435 1104 7 0.07917265644649518 1104 8 0.033493820505050564 1104 9 -0.08130243261486925 1104 10-11 0.11228654163253537 1104 12-13 0.05087017322381371 1104 14-15 0.09256828149375451 1104 16-17 0.10315962780726551 1104 18-19 0.15541250711668653 1104 20-21 0.11278359162376717 1104 22-23 -0.016167893923089593 1104 24-25 0.008979444191560049 1104 26-27 0.1510075921244649 1104 28-29 0.06115867916470563 1104 30-31 -0.13917037392305076 1104 32-33 -0.14902445972203537 1104 34-35 -0.12752049824993605 1104 36-37 0.05272748081907963 1104 38-39 0.004336548298560672 1104 40-41 0.02577710847771897 1104 42-43 -0.1443874296390959 1104 44-45 -0.12704777052341854 1104 46-47 0.05115746554935896 1104 48-49 0.07360108663952758 1104 50-51 0.09367777184157688 1104 52-53 -0.029254381963163212 1104 54-55 -0.0674722333983695 1104 56-57 0.024567015786821855 1104 58-59 0.0897340447078463 1104 60-61 0.1979054924178314 1104 62-63 0.14154212679139988 1104 64-65 -0.03338516214463283 1104 66-67 -0.14727441228917115 1104 68-69 0.031352286662666984 1104 70-71 -0.08889813528126922 1104 72-73 -0.10542553270265032 1104 74-75 0.09712721066605567 1104 76-77 0.021119630455782357 1104 78-79 -0.0202436267901831 1104 80-81 0.11939861510836991 1104 82-83 -0.0034738718135081115 1104 84-85 0.059565181790066646 1104 86-87 0.2071120188963036 1104 88-89 0.23382024062324547 1104 90-91 0.14558742835704308 1104 92-93 0.22130258675542436 1104 94-95 0.12615700064980828 1104 96-97 -0.00248660330197481 1104 98-99 0.4536942954845138 1104 100-101 1.3449953950667997 1105 1 0.07158737360811429 1105 2 0.08449649414380644 1105 3 0.033345012689821374 1105 4 -0.017882451841337854 1105 5 -0.08133998284962018 1105 6 -0.006262451748014541 1105 7 0.03546928222294099 1105 8 0.09948392569592102 1105 9 0.07661263433896437 1105 10-11 0.04313212642511388 1105 12-13 0.03255736303559331 1105 14-15 -0.07548350238113954 1105 16-17 -0.11736273695847643 1105 18-19 -0.016640509707606554 1105 20-21 -0.011582375851340032 1105 22-23 -0.10456763484764764 1105 24-25 -0.029263446840680274 1105 26-27 -0.028528959362873252 1105 28-29 0.10826827119891647 1105 30-31 0.02367453977048939 1105 32-33 -0.014748076165517432 1105 34-35 0.0426362736076058 1105 36-37 -0.06240892396289155 1105 38-39 -0.0012195274888071594 1105 40-41 -0.081118824349943 1105 42-43 -0.01791452331203658 1105 44-45 0.053161830874962845 1105 46-47 0.11552054573788695 1105 48-49 0.02156359873492164 1105 50-51 0.01932233906073577 1105 52-53 0.1908797598104215 1105 54-55 0.23353714638125922 1105 56-57 0.18447001856485912 1105 58-59 0.03454785374115232 1105 60-61 0.053755759296748806 1105 62-63 -0.013604656615278543 1105 64-65 -0.09294130035981851 1105 66-67 -0.26979875913812634 1105 68-69 -0.21157191035953105 1105 70-71 -0.14977501201753896 1105 72-73 0.02216131999891502 1105 74-75 -0.046786467268834286 1105 76-77 -0.05651521363074252 1105 78-79 -0.19669780477189036 1105 80-81 -0.057305274144685114 1105 82-83 0.03602147731406191 1105 84-85 0.09873278487399872 1105 86-87 0.12240722443989327 1105 88-89 0.019868403684704106 1105 90-91 -0.1080938620259495 1105 92-93 0.04848873090125849 1105 94-95 0.2582913297068359 1105 96-97 0.2339470515346207 1105 98-99 0.34509330288221207 1105 100-101 0.7926769819819306 1106 1 0.05086580529465934 1106 2 0.004985572151497308 1106 3 0.11047765131657883 1106 4 -0.009445980127182452 1106 5 -0.09005336491154736 1106 6 0.009753070482979354 1106 7 0.04666484860707243 1106 8 0.019524513794401344 1106 9 0.04526790812275294 1106 10-11 0.03394642395932834 1106 12-13 0.08655082966718197 1106 14-15 0.046907773370087114 1106 16-17 0.22383680435216746 1106 18-19 -0.050783174300072176 1106 20-21 0.021897562685047944 1106 22-23 0.06048856699092653 1106 24-25 0.16171628198735277 1106 26-27 0.04221543856444754 1106 28-29 0.12657199752570136 1106 30-31 0.14462192585119027 1106 32-33 0.11464364569257413 1106 34-35 0.2189743082209219 1106 36-37 0.16071838044594244 1106 38-39 0.3586435806177519 1106 40-41 0.49694439509269017 1106 42-43 0.45841798262652134 1106 44-45 0.45404556093837556 1106 46-47 0.549723835108729 1106 48-49 0.4370940274169257 1106 50-51 0.5257429892328886 1106 52-53 0.45645537111026613 1106 54-55 0.5941032540798474 1106 56-57 0.5856070667863449 1106 58-59 0.6326296419052184 1106 60-61 0.5044735814564376 1106 62-63 0.5550593391295422 1106 64-65 0.6168945456608697 1106 66-67 0.5174468741620402 1106 68-69 0.33191755084251895 1106 70-71 0.44167379626708936 1106 72-73 0.31435988330605724 1106 74-75 0.46595331516828153 1106 76-77 0.3779130356095415 1106 78-79 0.19267937095226984 1106 80-81 0.3778717292373379 1106 82-83 0.5289783746217864 1106 84-85 0.406250066748715 1106 86-87 0.626581612186822 1106 88-89 0.6757095915490723 1106 90-91 0.6382891454968629 1106 92-93 0.8163198985192359 1106 94-95 0.941987029541739 1106 96-97 1.0695619164252435 1106 98-99 1.3560970337844545 1106 100-101 1.8744341663994568 1107 1 -0.021634285512064366 1107 2 -0.08786843374598163 1107 3 -0.059288001286603276 1107 4 -0.02720117179541859 1107 5 -0.04605998224539576 1107 6 -0.07796622776263717 1107 7 0.016559942071957323 1107 8 -0.06724048830241003 1107 9 0.0190066026852449 1107 10-11 -0.2187620930512466 1107 12-13 -0.13589923488814293 1107 14-15 -0.2130950003626566 1107 16-17 -0.013827514547848807 1107 18-19 -0.17186321300024332 1107 20-21 -0.18643783443753392 1107 22-23 -0.1325946020647848 1107 24-25 -0.13490636818220736 1107 26-27 -0.23589089446179656 1107 28-29 -0.059311023597594215 1107 30-31 -0.14529918786854523 1107 32-33 -0.2568334660591418 1107 34-35 -0.23199996540781598 1107 36-37 -0.27657709803946773 1107 38-39 -0.2786462843608959 1107 40-41 -0.5608152873809544 1107 42-43 -0.4730840266786913 1107 44-45 -0.33152178298137613 1107 46-47 -0.5168044751266763 1107 48-49 -0.33930183707963124 1107 50-51 -0.5421589791267465 1107 52-53 -0.6113855090875902 1107 54-55 -0.3950313082812613 1107 56-57 -0.401850047673058 1107 58-59 -0.532569257847463 1107 60-61 -0.43472553623237786 1107 62-63 -0.48017201990044356 1107 64-65 -0.42186671850085133 1107 66-67 -0.3861461003691531 1107 68-69 -0.643394576643523 1107 70-71 -0.5861114111519896 1107 72-73 -0.5896392449493675 1107 74-75 -0.4515033700229587 1107 76-77 -0.6038166509256335 1107 78-79 -0.34768009160398705 1107 80-81 -0.3638178042142073 1107 82-83 -0.1376503183226916 1107 84-85 -0.4375706180330674 1107 86-87 -0.5226848345624582 1107 88-89 -0.6037076604082259 1107 90-91 -0.24197310433060792 1107 92-93 -0.018968255990884586 1107 94-95 -0.06629784602002786 1107 96-97 0.143440618450402 1107 98-99 0.2631835907565936 1107 100-101 0.9649205932703211 1108 1 0.14427535662848 1108 2 0.12830249210236389 1108 3 0.0969254796297605 1108 4 0.06726887428752804 1108 5 0.007045655749884361 1108 6 0.06533190025535873 1108 7 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sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 16.0 5 68.0 6 203.0 7 396.0 8 674.0 9 1207.0 10 2113.0 11 3149.0 12 4514.0 13 6171.0 14 7285.0 15 9503.0 16 11915.0 17 16374.0 18 15152.0 19 10168.0 20 9490.0 21 9828.0 22 10639.0 23 11764.0 24 13464.0 25 15690.0 26 18124.0 27 21740.0 28 26235.0 29 32096.0 30 38743.0 31 46240.0 32 56384.0 33 71031.0 34 93273.0 35 133731.0 36 189307.0 37 299532.0 38 241108.0 39 19206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.564380672460466 21.89206848074542 15.424588797499009 18.118962049295106 2 13.608324962790547 26.299911305659503 36.99971172611077 23.092052005439182 3 23.529301969318535 28.39960982785832 32.30126211424356 15.769826088579583 4 10.935535183586225 18.528544991120196 42.7812277702464 27.754692055047176 5 9.607227565986461 43.07641774944162 35.669407314695086 11.646947369876843 6 23.392296795896822 41.52959338093826 17.671202477170585 17.406907345994334 7 22.36417771774184 37.77839687917591 23.04449027023776 16.81293513284449 8 22.135217736655928 30.67241094445301 21.85295819475563 25.33941312413544 9 22.26476816073654 22.427224669055352 25.77154743626347 29.536459733944632 10-11 22.436107929191085 26.538929764175368 28.818713054106315 22.206249252527236 12-13 28.907407394605478 25.98529872295358 26.89111473508183 18.216179147359114 14-15 23.66376065386325 28.211609625997465 30.799403542657966 17.325226177481316 16-17 17.25002799857864 31.16940038797043 31.842956318069383 19.73761529538155 18-19 16.608620149722682 34.20086260949396 31.643382385981454 17.547134854801904 20-21 19.476634260625865 29.733032109010395 28.98635434460551 21.80397928575823 22-23 18.129904371877466 29.298167144934016 29.771641453647014 22.800287029541504 24-25 21.47255337755395 29.98776386330092 29.776085611478464 18.763597147666665 26-27 21.213824360412826 26.57096104510207 34.364049141421575 17.851165453063526 28-29 17.44979921066876 31.26482415638409 33.00518270826752 18.280193924679633 30-31 18.30646348772693 30.482682481865357 31.186914162820134 20.023939867587575 32-33 18.420978043484425 30.88509070949251 31.444183054610964 19.249748192412106 34-35 20.899697326266796 27.980866152946334 30.92377710271134 20.195659418075525 36-37 20.613476486604966 27.361330639529495 33.62521970127541 18.39997317259013 38-39 20.21570442727149 28.64053940626316 33.37932326694235 17.764432899523 40-41 18.085790760532337 29.972321212211302 33.57156610363074 18.370321923625628 42-43 16.985702251045947 31.407378165279503 30.309527027657662 21.297392556016888 44-45 18.79863088608715 29.295090229197303 29.98541980790649 21.92085907680906 46-47 20.570901795738234 30.34044780081519 29.189214786220326 19.899435617226256 48-49 20.003885209346763 29.11290369969406 30.60411203091023 20.279099060048953 50-51 17.798464681770056 30.233961648498607 33.03592260292747 18.931651066803866 52-53 18.335118050577204 31.870890628056863 30.468165091760273 19.32582622960566 54-55 16.50164081073402 28.781313491978448 32.01208952943608 22.70495616785145 56-57 19.87551533037659 29.90580519431589 28.965723681640842 21.25295579366668 58-59 21.22223627878167 28.35022540038727 29.44805421499761 20.97948410583345 60-61 18.7985523727882 29.263431512507733 32.035561577733915 19.902454536970147 62-63 19.523362311907437 28.88916464487219 31.255104894234716 20.33236814898566 64-65 18.57911748673451 31.203204387920913 30.61012439194437 19.607553733400206 66-67 18.35196166069689 29.144396672411755 29.326912108447356 23.176729558444002 68-69 17.089699682967193 30.408050116899798 30.123577811298425 22.378672388834584 70-71 20.03600312746061 29.865831663257385 28.21368353886962 21.884481670412384 72-73 18.955805607998666 27.147035583505758 30.801615716388262 23.09554309210731 74-75 17.44716343042966 31.820708229867765 28.72534800378283 22.006780335919743 76-77 19.195467136009782 27.660970382341944 29.66289539004578 23.48066709160249 78-79 18.367275590823734 27.90861810849869 31.14821000527869 22.575896295398888 80-81 19.546735355278294 28.97220102203345 29.308281623307668 22.172781999380586 82-83 18.299001677811752 28.173514908466966 30.640700104457093 22.88678330926419 84-85 17.680176365550647 27.907781390226756 29.965310350691755 24.446731893530842 86-87 18.313870002249097 28.710748948980118 29.612947872874173 23.36243317589661 88-89 18.858907588851803 27.296646286258593 28.96458831830067 24.879857806588934 90-91 18.505151115464866 25.801381982043882 30.555489046267216 25.137977856224037 92-93 19.185473972449326 28.559705973137866 29.872222197258342 22.38259785715446 94-95 18.375949465534077 27.477165299897166 29.552500367256297 24.59438486731246 96-97 16.1946092054143 27.169003276784604 30.492001603827063 26.144385913974034 98-99 18.057455682210183 27.639900977573394 28.25989723070414 26.042746109512276 100-101 18.16778140716545 25.97276230967318 29.780335631945526 26.07912065121584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7834.0 1 4758.5 2 1846.0 3 1977.5 4 1580.0 5 1545.0 6 2760.5 7 4898.5 8 6568.0 9 6762.0 10 5335.5 11 3879.5 12 3468.0 13 3188.5 14 2917.5 15 2642.0 16 2548.0 17 2550.5 18 2570.5 19 2690.0 20 3018.0 21 3419.5 22 3805.0 23 4361.0 24 4818.0 25 5988.0 26 9004.0 27 12000.0 28 12578.5 29 13648.5 30 17275.0 31 20929.0 32 26838.5 33 34578.0 34 41504.0 35 46931.5 36 54691.5 37 61729.0 38 59196.0 39 53179.5 40 48480.0 41 42846.0 42 36326.0 43 34787.0 44 36252.5 45 36415.0 46 43603.5 47 79826.0 48 171292.0 49 163098.5 50 74894.5 51 48146.5 52 28959.0 53 12706.0 54 5647.5 55 4082.5 56 3520.5 57 3067.0 58 2631.0 59 2365.5 60 2101.0 61 1720.0 62 1334.0 63 980.5 64 727.5 65 576.0 66 483.5 67 409.5 68 372.5 69 290.5 70 182.5 71 120.5 72 89.0 73 94.5 74 77.5 75 48.0 76 32.0 77 19.0 78 17.5 79 10.5 80 4.5 81 3.5 82 1.5 83 0.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22633302478118492 2 0.0 3 0.0 4 0.0 5 0.02841260634852382 6 0.0035947823117353744 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0026960867338015307 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.147825744310047E-4 26-27 0.0015208694395803506 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006843912478111578 36-37 0.017904781129605037 38-39 0.014517390105085164 40-41 0.008744999277587016 42-43 0.008606738419443349 44-45 0.004009564886166379 46-47 3.456521453591706E-4 48-49 0.0020393476576191063 50-51 3.456521453591706E-5 52-53 0.0 54-55 0.0 56-57 0.0012097825087570972 58-59 2.7652171628733647E-4 60-61 0.002488695446586028 62-63 8.641303633979265E-4 64-65 0.001693695512259936 66-67 0.0060834777583214025 68-69 2.0739128721550235E-4 70-71 0.0 72-73 0.0 74-75 3.456521453591706E-4 76-77 0.05755108220230191 78-79 0.14195933609901137 80-81 8.986955779338436E-4 82-83 9.678260070056776E-4 84-85 0.0 86-87 0.10480173047290053 88-89 0.354224318564078 90-91 0.3898610547506085 92-93 4.493477889669218E-4 94-95 2.419565017514194E-4 96-97 6.913042907183412E-5 98-99 0.0 100-101 0.0011060868651493459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1446541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.360845081480418 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57151022833825 11.608422611910168 2 9.672953882346082 2.9716949213404544 3 3.5669879062485523 1.643758459061946 4 1.915646983411497 1.1770382617195718 5 1.2925991628253013 0.9927707746305368 6 0.8680568177594347 0.8000451779715333 7 0.6863129124390652 0.7379642427777281 8 0.5305676648621932 0.6519974164152776 9 0.4424998770996331 0.611745485463143 >10 3.920673699483602 12.703875002360062 >50 0.7591083898249072 8.235918688011639 >100 0.6770669842524223 20.312962607845332 >500 0.056797896149004795 5.7389414737021065 >1k 0.02479273244599416 7.481306844568361 >5k 0.008564762117707073 9.315627117970498 >10k+ 0.005860100396325892 15.015930914251655 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 43047 2.9758575802552434 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23040 1.592765085815058 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22902 1.583225086603145 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 19582 1.3537120620846559 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 16377 1.1321490369094274 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13142 0.908512098862044 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12767 0.8825881879601063 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12295 0.8499586254382006 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11236 0.7767495010511282 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10992 0.7598816763576006 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10960 0.7576695026273019 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10261 0.7093473327060899 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10257 0.7090708109898026 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9890 0.6836999435204394 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9625 0.6653803798164034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9306 0.6433277729424883 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8634 0.5968721246062159 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8629 0.5965264724608567 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8018 0.554287780297966 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7929 0.5481351721105727 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7544 0.5215199569179165 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7220 0.49912169789864236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6805 0.4704325698338312 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6667 0.46089257062191813 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6182 0.42736431252207857 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5956 0.41174083555184404 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5899 0.4078004010947495 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5523 0.3818073597637398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5465 0.3777977948775735 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5222 0.3609991006131178 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5016 0.34675823222431995 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5005 0.3459977975045298 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4690 0.324221712346902 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4378 0.3026530184764898 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4332 0.2994730187391854 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4287 0.29636214943095285 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3812 0.2635251956218317 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3710 0.25647389185650454 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3646 0.2520495443959072 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3555 0.2457586753503703 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3474 0.24015911059555173 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3339 0.2308265026708541 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2638 0.18236607189149842 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.18153650674263638 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2545 0.17593694198781784 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2518 0.17407042040287832 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2396 0.16563650805611455 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2167 0.14980563979866454 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2157 0.1491143355079462 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2114 0.14614172705785733 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2018 0.13950520586696125 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1950 0.13480433669007655 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.12955042408061715 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1840 0.1271999894921748 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1796 0.12415825061301408 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.12180781602457172 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1662 0.11489477311738831 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1584 0.10950259964978525 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1490 0.10300433931703283 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.10279694802981733 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1454 0.1005156438704468 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0369564360775118E-4 0.0 12-13 0.0 0.0 0.0 1.3826085814366823E-4 0.0 14-15 0.0 0.0 0.0 2.4195650175141942E-4 0.0 16-17 0.0 0.0 0.0 5.530434325746729E-4 0.0 18-19 0.0 0.0 0.0 9.332607924697606E-4 0.0 20-21 0.0 0.0 0.0 0.001659130297724019 0.0 22-23 0.0 0.0 0.0 0.0038021735989508766 0.0 24-25 0.0 0.0 0.0 0.007569781983365836 0.0 26-27 0.0 0.0 0.0 0.015796303042914097 0.0 28-29 0.0 0.0 0.0 0.0423423878064984 0.0 30-31 0.0 0.0 0.0 0.10338455667692792 0.0 32-33 0.0 0.0 0.0 0.2325893286121859 0.0 34-35 0.0 0.0 0.0 0.4530462669222649 0.0 36-37 0.0 0.0 0.0 0.7612297197245014 0.0 38-39 0.0 0.0 0.0 1.1443505576406061 0.0 40-41 0.0 0.0 0.0 1.5461711766206419 0.0 42-43 0.0 0.0 0.0 1.9608500554080388 6.913042907183412E-5 44-45 0.0 0.0 0.0 2.432112190390732 6.913042907183412E-5 46-47 0.0 0.0 0.0 3.0193751853559627 6.913042907183412E-5 48-49 0.0 0.0 0.0 3.588940790478804 6.913042907183412E-5 50-51 0.0 0.0 0.0 4.15601770015506 6.913042907183412E-5 52-53 0.0 0.0 0.0 4.664333745120256 6.913042907183412E-5 54-55 0.0 0.0 0.0 5.215960003898957 6.913042907183412E-5 56-57 0.0 0.0 0.0 5.933464727235522 6.913042907183412E-5 58-59 0.0 0.0 0.0 6.664138797310273 6.913042907183412E-5 60-61 0.0 0.0 0.0 7.536495681767748 6.913042907183412E-5 62-63 0.0 0.0 0.0 8.31922496493359 6.913042907183412E-5 64-65 0.0 0.0 0.0 9.029540123646685 6.913042907183412E-5 66-67 0.0 0.0 0.0 9.849150490722351 6.913042907183412E-5 68-69 0.0 0.0 0.0 10.765785415000334 6.913042907183412E-5 70-71 0.0 0.0 0.0 11.741146638774843 6.913042907183412E-5 72-73 0.0 0.0 0.0 12.684223952172804 6.913042907183412E-5 74-75 0.0 0.0 0.0 13.727021909506885 6.913042907183412E-5 76-77 0.0 0.0 0.0 14.645834442300632 6.913042907183412E-5 78-79 0.0 0.0 0.0 15.853128255611143 6.913042907183412E-5 80-81 0.0 0.0 0.0 17.075008589455813 6.913042907183412E-5 82-83 0.0 0.0 0.0 18.125825676562226 6.913042907183412E-5 84-85 0.0 0.0 0.0 19.397721875840368 6.913042907183412E-5 86-87 0.0 0.0 0.0 20.703595681007315 6.913042907183412E-5 88-89 0.0 0.0 0.0 21.904633190486823 6.913042907183412E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGATCG 45 9.6861436E-4 42.168728 7 TAGGCAT 285 0.0 36.637943 5 GTAAGCG 175 0.0 36.597065 94-95 TAGACCG 65 1.3344931E-4 36.50984 4 GACCGGT 65 1.3380268E-4 36.49343 6 TACCTGG 215 0.0 35.321144 2 GTATGCG 35 2.0692436E-4 33.88617 94-95 CTTTAGG 300 0.0 33.223953 2 CATTCCG 315 0.0 33.132572 9 CCTTTAG 320 0.0 32.700783 1 TTAGGCA 310 0.0 32.152214 4 GGGATGC 230 0.0 30.939013 7 GGCATTC 355 0.0 29.399326 7 GGGTTAC 65 0.0058808834 29.193735 7 ATGTATT 115 3.8246744E-6 28.95247 1 GTACCGG 85 6.35712E-4 27.97928 1 CTTATGT 275 0.0 27.614712 2 TACTCCC 155 4.624235E-8 27.55904 5 TGGGTAT 285 0.0 26.633804 6 CGGTGTA 90 9.0151466E-4 26.355455 9 >>END_MODULE