FastQCFastQC Report
Thu 26 May 2016
SRR938803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811786
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66430.818319113658033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.5910424668570289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.4026923351720774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16640.2049801302313664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.13710509912710986No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.10704791656914506No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.1068015462203093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG459.751371E-442.1090282
GGTATCA35200.041.4664151
GTATCAA57050.036.0514341
ACCGCTT650.005932717629.1398268
TATCAAC70700.028.8792292
ATCAACG70600.028.8530333
TCAACGC71300.028.6362084
CAACGCA72400.028.2665585
AACGCAG73600.027.7259376
GTACATG43150.026.5780471
GTACTAT909.066948E-426.3278831
GTCTAGA2059.822543E-1025.4288831
TACATGG43550.025.236412
ACATGGG43500.024.938713
ACGCAGA82300.024.510387
CGCAGAG82800.024.2479958
GAGTACT48250.023.55416112-13
GCAGAGT84600.023.2283339
CATGGGG13400.022.6257464
CATGGGA27900.022.073284
Produced by FastQC (version 0.11.5)