##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938803_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811786 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52979972554343 33.0 31.0 34.0 30.0 34.0 2 31.579774718953026 33.0 31.0 34.0 30.0 34.0 3 31.57488919493561 33.0 31.0 34.0 28.0 34.0 4 35.150830391260754 37.0 35.0 37.0 33.0 37.0 5 35.188516431670415 37.0 35.0 37.0 33.0 37.0 6 35.28123298504779 37.0 35.0 37.0 33.0 37.0 7 35.198945288536635 37.0 35.0 37.0 32.0 37.0 8 35.17781410371699 37.0 35.0 37.0 32.0 37.0 9 36.73508412315561 39.0 37.0 39.0 32.0 39.0 10-11 36.64132049579569 39.0 37.0 39.0 32.0 39.0 12-13 36.52606105549985 39.0 37.0 39.0 32.0 39.0 14-15 37.64698147048607 40.0 37.0 41.0 32.0 41.0 16-17 37.59385355253725 40.0 37.0 41.0 32.0 41.0 18-19 37.58302865533527 40.0 37.0 41.0 32.0 41.0 20-21 37.495929961837234 40.0 37.0 41.0 32.0 41.0 22-23 37.373764760663526 40.0 37.0 41.0 32.0 41.0 24-25 37.333385892341084 40.0 37.0 41.0 32.0 41.0 26-27 37.21911624492169 40.0 37.0 41.0 31.0 41.0 28-29 37.075460774144915 40.0 36.0 41.0 31.0 41.0 30-31 36.80726632388338 39.0 36.0 41.0 30.5 41.0 32-33 36.51802630249844 39.0 35.0 41.0 30.0 41.0 34-35 36.14435393071573 39.0 35.0 41.0 30.0 41.0 36-37 35.83964246734977 39.0 35.0 41.0 29.0 41.0 38-39 35.6246855698423 39.0 35.0 41.0 27.5 41.0 40-41 35.390132374788436 38.5 35.0 40.0 26.5 41.0 42-43 35.04177517227447 38.0 35.0 40.0 25.5 41.0 44-45 34.85436802309968 38.5 35.0 40.5 24.0 41.0 46-47 34.50585006393311 39.0 34.5 41.0 22.5 41.0 48-49 34.158697612424945 38.5 34.0 41.0 19.0 41.0 50-51 32.96085298342174 37.0 32.5 39.5 14.0 40.5 52-53 33.019311739793494 37.5 33.0 39.5 13.5 40.5 54-55 33.49708359099566 38.0 33.0 40.0 11.5 41.0 56-57 33.39783206411542 38.0 33.0 40.0 8.5 41.0 58-59 33.171568615374 38.0 33.0 40.0 7.5 41.0 60-61 32.886465768071886 37.0 33.0 40.0 4.0 41.0 62-63 32.52911296819605 37.0 32.5 40.0 2.0 41.0 64-65 32.13333871241928 36.0 32.0 39.5 2.0 41.0 66-67 31.735542372004446 36.0 31.5 39.0 2.0 41.0 68-69 31.323186775825157 35.0 31.0 39.0 2.0 40.5 70-71 30.902836461826148 35.0 31.0 38.5 2.0 40.0 72-73 30.471321875469645 35.0 30.5 37.5 2.0 40.0 74-75 29.998005632026175 35.0 30.0 37.0 2.0 39.0 76-77 29.528171217537626 35.0 29.0 36.5 2.0 39.0 78-79 29.06430820437899 34.5 29.0 36.0 2.0 38.5 80-81 28.59044748246459 34.0 29.0 35.5 2.0 37.0 82-83 28.077793285422512 34.0 27.5 35.0 2.0 37.0 84-85 27.622109151919346 34.0 26.5 35.0 2.0 36.5 86-87 27.184331831295438 34.0 26.0 35.0 2.0 36.0 88-89 26.75935098659006 34.0 25.0 35.0 2.0 36.0 90-91 26.28170416833993 33.5 24.0 35.0 2.0 35.0 92-93 25.779533645566687 33.0 21.5 35.0 2.0 35.0 94-95 25.21934906490134 33.0 17.5 35.0 2.0 35.0 96-97 24.590647042447145 33.0 3.5 35.0 2.0 35.0 98-99 23.880357631198372 32.0 2.0 35.0 2.0 35.0 100-101 21.894434617004976 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3106746996744256 1101 2 0.11419077304381275 1101 3 0.2427116815771342 1101 4 0.28094533308314595 1101 5 0.1079871865357589 1101 6 0.053710600591784896 1101 7 0.0646844787678873 1101 8 0.16481961126537215 1101 9 0.20716072365353 1101 10-11 0.3336216915617385 1101 12-13 0.2668441312417613 1101 14-15 0.3540485736754917 1101 16-17 0.23970748068809655 1101 18-19 0.5152404129644736 1101 20-21 0.41977044765224036 1101 22-23 0.3333250534683927 1101 24-25 0.4790310391217929 1101 26-27 0.2797925934752854 1101 28-29 0.06776491577858934 1101 30-31 0.02376658438267043 1101 32-33 -0.05311740494330763 1101 34-35 0.07392057750890046 1101 36-37 -0.023185632856886684 1101 38-39 -0.050104638201368346 1101 40-41 0.14317566952946947 1101 42-43 0.3409380098089372 1101 44-45 0.36230654559378195 1101 46-47 0.32410171046826264 1101 48-49 -0.05210281822541418 1101 50-51 -0.06129093384240747 1101 52-53 -0.11621255625495763 1101 54-55 -0.3362401235946848 1101 56-57 -0.14752380915537344 1101 58-59 -0.08638980099477322 1101 60-61 -0.1399563929678962 1101 62-63 -0.10234215941763836 1101 64-65 0.03503342720919278 1101 66-67 -0.10034274779925667 1101 68-69 -0.1362263683607985 1101 70-71 -0.5154509211138212 1101 72-73 -0.36706903271282343 1101 74-75 -0.5877981677140518 1101 76-77 -0.570768855438061 1101 78-79 -0.4271170413199705 1101 80-81 -0.4860667641989771 1101 82-83 -0.4772577092879082 1101 84-85 -0.619261427019719 1101 86-87 -0.9696433336805406 1101 88-89 -1.1686679460449554 1101 90-91 -1.1575514002395515 1101 92-93 -1.2092641424856119 1101 94-95 -1.4543397030033738 1101 96-97 -1.391856672547572 1101 98-99 -1.5578521077731189 1101 100-101 -1.447125646521684 1102 1 0.3467505404431286 1102 2 0.46441097437073964 1102 3 0.4652580096485117 1102 4 0.380164619287676 1102 5 0.3029797511662764 1102 6 0.31736594261877826 1102 7 0.3807591756335924 1102 8 0.31389876935276817 1102 9 0.48681640885142485 1102 10-11 0.6169307169563822 1102 12-13 0.5551934792170599 1102 14-15 0.440692076306469 1102 16-17 0.5422197776453146 1102 18-19 0.5842892576224443 1102 20-21 0.494145590640116 1102 22-23 0.3022108919492936 1102 24-25 0.3362576463054978 1102 26-27 0.4256116280211515 1102 28-29 0.6403241127586838 1102 30-31 0.699534429269427 1102 32-33 0.49820809265450094 1102 34-35 0.6122985230591169 1102 36-37 0.5979322463469572 1102 38-39 0.6608882696000435 1102 40-41 0.6490596205704193 1102 42-43 0.6730059004518125 1102 44-45 0.7151505744201359 1102 46-47 0.6144671874758174 1102 48-49 0.8039798859612617 1102 50-51 0.6552927998569729 1102 52-53 0.6259946045778051 1102 54-55 0.5924518233282186 1102 56-57 0.47149214829143204 1102 58-59 0.3942210432056328 1102 60-61 0.3049789765127784 1102 62-63 0.4352565022158501 1102 64-65 0.498321290398394 1102 66-67 0.409301784344418 1102 68-69 0.5157268460066255 1102 70-71 0.558716889455841 1102 72-73 0.5144798119451508 1102 74-75 0.3995331065938892 1102 76-77 0.3815017920110364 1102 78-79 0.007487454218807699 1102 80-81 0.12962472516272427 1102 82-83 0.05864654603124109 1102 84-85 0.07600996396632453 1102 86-87 -0.18928700789696862 1102 88-89 -0.25033547063886985 1102 90-91 -0.27425810351111224 1102 92-93 -0.5752130100216455 1102 94-95 -0.4578357565380351 1102 96-97 -0.4963450619321499 1102 98-99 -0.2836442605985603 1102 100-101 -0.3902879663569614 1103 1 0.09522478456406702 1103 2 0.07041601356447202 1103 3 0.14759283607628504 1103 4 0.1838740257826359 1103 5 0.09518299864667767 1103 6 0.1978390669188883 1103 7 -0.07560980532944939 1103 8 0.03878678726989904 1103 9 0.3316372375188408 1103 10-11 0.23243323655324133 1103 12-13 0.36905126197181914 1103 14-15 0.3084062420399434 1103 16-17 0.4238897104560664 1103 18-19 0.4255686529191962 1103 20-21 0.2886654674598219 1103 22-23 0.3756991338305866 1103 24-25 0.3300426407063952 1103 26-27 0.2795449986818497 1103 28-29 0.27451364243393783 1103 30-31 0.2814703195269814 1103 32-33 0.25634779030287547 1103 34-35 0.563026406597757 1103 36-37 0.5125529070412398 1103 38-39 0.3417813267109082 1103 40-41 0.3074512779052512 1103 42-43 0.23558288813379136 1103 44-45 0.5289873605343658 1103 46-47 0.5559565491779423 1103 48-49 0.5453292700598169 1103 50-51 0.5561326654784793 1103 52-53 0.509155298866709 1103 54-55 0.5134344661053731 1103 56-57 0.4866867171604099 1103 58-59 0.6180032380544631 1103 60-61 0.5642314961096417 1103 62-63 0.45956361307811733 1103 64-65 0.2840925669941399 1103 66-67 0.17795380002190697 1103 68-69 0.14869157165052016 1103 70-71 -0.10749251703911966 1103 72-73 0.2702328914524301 1103 74-75 0.39765740670587135 1103 76-77 0.21175095214541528 1103 78-79 0.013863434062031388 1103 80-81 -0.09790886946213462 1103 82-83 0.022084395975252136 1103 84-85 -0.08019804275259901 1103 86-87 -0.0648329205509448 1103 88-89 -0.2552836789255437 1103 90-91 -0.15956045513485506 1103 92-93 -0.4577997961358662 1103 94-95 -0.33494241947196457 1103 96-97 -0.4196863273296856 1103 98-99 -0.4145513154697831 1103 100-101 -0.36556792156412854 1104 1 0.3037517838805428 1104 2 0.5540455419308437 1104 3 0.4575210657413571 1104 4 0.5744413531920713 1104 5 0.5629376124539291 1104 6 0.6084166472333123 1104 7 0.3912903997286463 1104 8 0.4902734584934123 1104 9 0.6045353919959382 1104 10-11 0.5169428059312295 1104 12-13 0.4432737500100927 1104 14-15 0.7766222365717326 1104 16-17 0.8081685894717907 1104 18-19 0.8756370056191258 1104 20-21 0.9196377846163486 1104 22-23 0.7415484161272374 1104 24-25 0.7773256208841133 1104 26-27 0.8835350875403591 1104 28-29 0.6266411202193041 1104 30-31 0.5001647829582865 1104 32-33 0.37066768093855273 1104 34-35 0.4629409657228365 1104 36-37 0.741115791525182 1104 38-39 0.7450793444826118 1104 40-41 0.5563320024438667 1104 42-43 0.39655349647281213 1104 44-45 0.5427946059205553 1104 46-47 0.4608266790480755 1104 48-49 0.4271389077904999 1104 50-51 0.7039112027307013 1104 52-53 0.8085657568298004 1104 54-55 0.8757379520930684 1104 56-57 0.7140021648706067 1104 58-59 0.7616464369608096 1104 60-61 0.6537735330201002 1104 62-63 0.8176463198450321 1104 64-65 0.6769497098512787 1104 66-67 0.5859510659139069 1104 68-69 0.5330900678911235 1104 70-71 0.18002457113586345 1104 72-73 0.30566522227949733 1104 74-75 0.2291338250243946 1104 76-77 0.3920226541276435 1104 78-79 0.3653159289355763 1104 80-81 0.27205011891790676 1104 82-83 0.1646254075952065 1104 84-85 0.2898207542398836 1104 86-87 0.33693570555978525 1104 88-89 0.32309123563358 1104 90-91 0.4431223883374642 1104 92-93 0.5113475039324875 1104 94-95 0.47190139460868963 1104 96-97 0.5295788811460476 1104 98-99 0.6707699422198949 1104 100-101 0.8345773279232276 1105 1 0.10525641735448588 1105 2 0.15029890493612186 1105 3 0.16909976836865326 1105 4 0.16957869666700986 1105 5 0.04758237945163302 1105 6 0.32100840330333114 1105 7 0.07493872071712104 1105 8 0.14597146892288038 1105 9 0.2102682780357341 1105 10-11 0.2111534546175804 1105 12-13 0.081881050637854 1105 14-15 0.04952886958066216 1105 16-17 -0.2689895465098786 1105 18-19 -0.1920582147615164 1105 20-21 0.04066943838457604 1105 22-23 0.1971168885755077 1105 24-25 0.20139477423715846 1105 26-27 0.16580997377943874 1105 28-29 0.014288511191509201 1105 30-31 0.1351383771390431 1105 32-33 0.0491328219356717 1105 34-35 0.22076970986871913 1105 36-37 0.08440140616629321 1105 38-39 0.19930925779180342 1105 40-41 0.2803463512664024 1105 42-43 0.3133120576047759 1105 44-45 0.27053400207427103 1105 46-47 0.29284913229814435 1105 48-49 0.3739552627910001 1105 50-51 0.30733067328571195 1105 52-53 0.23741785102716761 1105 54-55 0.03561107130122565 1105 56-57 0.20365905529475015 1105 58-59 0.4128006535881781 1105 60-61 0.5826256272174604 1105 62-63 0.3743872770974974 1105 64-65 0.3519953802933671 1105 66-67 0.2418251151383437 1105 68-69 0.3078617156806729 1105 70-71 0.1305560090074529 1105 72-73 0.13982671296272642 1105 74-75 0.31085568372835226 1105 76-77 0.4452295495340266 1105 78-79 0.1277515751308158 1105 80-81 0.20287185472685465 1105 82-83 0.3715274569525029 1105 84-85 0.14385802132062508 1105 86-87 0.18580141785260906 1105 88-89 0.3294568648892344 1105 90-91 0.4413918937680954 1105 92-93 0.2646241505595164 1105 94-95 0.24868088110684639 1105 96-97 0.24291631097964128 1105 98-99 -0.00785795162538605 1105 100-101 -0.08903844249561388 1106 1 -0.041769532849002644 1106 2 0.1618177183405507 1106 3 0.19869891103231296 1106 4 0.3537021951778456 1106 5 0.07964044689455108 1106 6 -0.01262298151839758 1106 7 -0.08676188217967962 1106 8 -0.001366777876896208 1106 9 -0.0996778754564005 1106 10-11 0.21499141541943345 1106 12-13 0.04729338076494827 1106 14-15 0.17479103435492505 1106 16-17 0.1255741915817623 1106 18-19 0.07877525554400222 1106 20-21 0.09371383709856929 1106 22-23 0.11537407632822294 1106 24-25 0.16903262852942902 1106 26-27 -0.027356976693603485 1106 28-29 0.2507972982315323 1106 30-31 0.11117536620538004 1106 32-33 0.056665744571574805 1106 34-35 -0.05152169244161797 1106 36-37 0.027704780941931517 1106 38-39 0.0828752246949378 1106 40-41 0.1213956020833038 1106 42-43 -0.016338367447325197 1106 44-45 -0.07237916632261943 1106 46-47 -0.1699431937526512 1106 48-49 -0.08514867609091681 1106 50-51 -0.05026700980226906 1106 52-53 -0.1011494636823258 1106 54-55 -0.34446703572117343 1106 56-57 -0.18881558293429634 1106 58-59 -0.16234448214578379 1106 60-61 -0.02650724019127182 1106 62-63 0.07653357654719883 1106 64-65 0.10144475208411308 1106 66-67 -0.0685960579134175 1106 68-69 -0.03450082450696712 1106 70-71 -0.006723000059007944 1106 72-73 0.08142724945540536 1106 74-75 0.2303390465381021 1106 76-77 0.1284046525919038 1106 78-79 0.32287777582904553 1106 80-81 0.17877899415578113 1106 82-83 0.05583997708741251 1106 84-85 0.04633929996150954 1106 86-87 0.35477031311161866 1106 88-89 0.25901918942785684 1106 90-91 0.10048892925156494 1106 92-93 0.0673135173535151 1106 94-95 -0.31799444220231265 1106 96-97 -0.17150949063641363 1106 98-99 0.23166367573573865 1106 100-101 0.2523000935894366 1107 1 -0.11085290967152162 1107 2 0.010140324341413987 1107 3 -0.07790841705654827 1107 4 0.01419984031898025 1107 5 0.07293989087725805 1107 6 -0.03204815112621873 1107 7 -0.19672509355000756 1107 8 0.003761724613262629 1107 9 0.16459463100255078 1107 10-11 0.1717502791597596 1107 12-13 0.2324284549542739 1107 14-15 0.04913305908255694 1107 16-17 0.053213018726737005 1107 18-19 0.2768217857512738 1107 20-21 0.3588722343771238 1107 22-23 0.4814322165373568 1107 24-25 0.3727118136387304 1107 26-27 0.3458043971780924 1107 28-29 0.26552241436375823 1107 30-31 0.2668086653916433 1107 32-33 0.38943049706979593 1107 34-35 0.2437908176253245 1107 36-37 0.23470202984826471 1107 38-39 0.26740789312695057 1107 40-41 0.17590365885762793 1107 42-43 0.44473075780797444 1107 44-45 0.3960299670506515 1107 46-47 0.1891019125864588 1107 48-49 0.31409493422021484 1107 50-51 0.216283041418329 1107 52-53 0.20436206578400373 1107 54-55 0.09175526811038281 1107 56-57 -0.002630325446098425 1107 58-59 0.061111007478018564 1107 60-61 0.07525906503195046 1107 62-63 0.06100471583500422 1107 64-65 -0.004253297667517586 1107 66-67 -0.11214645060465855 1107 68-69 0.029581295961300214 1107 70-71 0.30751374862503766 1107 72-73 0.13762010949754355 1107 74-75 0.23074011347278756 1107 76-77 0.03916323284716938 1107 78-79 -0.09309145315601342 1107 80-81 -0.17873593713131086 1107 82-83 0.19285507266697977 1107 84-85 0.2747831602549198 1107 86-87 0.3708500368425405 1107 88-89 -0.0236734032363195 1107 90-91 -0.21538752280402562 1107 92-93 -0.2948611996446395 1107 94-95 -0.4925552014268497 1107 96-97 -0.27689184111414633 1107 98-99 -0.3003282578256723 1107 100-101 -0.29673960076212325 1108 1 0.22583619135820854 1108 2 0.17143776269384858 1108 3 0.11993168882613503 1108 4 0.11598520260108813 1108 5 0.21927952272842077 1108 6 0.1729869084327902 1108 7 0.002921964169196656 1108 8 0.09289842403973125 1108 9 -0.05483033590910935 1108 10-11 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-1.5455781987104125 2316 82-83 -1.3720082290384248 2316 84-85 -1.3783556639365386 2316 86-87 -1.357962639338556 2316 88-89 -1.4340327214903752 2316 90-91 -1.6079711127560365 2316 92-93 -1.7875117449267641 2316 94-95 -1.909008236300938 2316 96-97 -1.8828141312469633 2316 98-99 -1.7169558455058898 2316 100-101 -1.8985625052166064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5434.0 3 1189.0 4 1050.0 5 1091.0 6 1160.0 7 1404.0 8 1630.0 9 1916.0 10 2481.0 11 3484.0 12 4762.0 13 6632.0 14 7054.0 15 7281.0 16 9296.0 17 12009.0 18 9857.0 19 6556.0 20 6480.0 21 6648.0 22 7041.0 23 7986.0 24 8808.0 25 10009.0 26 11440.0 27 13352.0 28 15590.0 29 18341.0 30 21448.0 31 25723.0 32 30870.0 33 38585.0 34 49752.0 35 69542.0 36 95954.0 37 137433.0 38 129500.0 39 22993.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69794037920317 19.905760596380702 14.436469834877464 21.95982918953866 2 15.779733032154017 22.016339635576312 39.30316114984082 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31.742411544017234 20.007789352251272 22-23 21.256180967765594 26.917124647385148 31.517558170279457 20.309136214569794 24-25 20.817830346157354 27.09416693172768 31.797582150421512 20.290420571693456 26-27 20.666081876031374 27.245592957361858 31.68336484339796 20.40496032320881 28-29 20.3858206176976 27.488640370016988 31.77799473869769 20.34754427358772 30-31 20.632119868948806 27.129458661926865 31.66626812246236 20.572153346661967 32-33 20.42886511029439 27.36980726520692 31.42786187380912 20.77346575068957 34-35 20.945222648231915 27.291634623284093 31.25686037238889 20.5062823560951 36-37 21.12474297167135 26.9993756446979 31.073464157117247 20.802417226513505 38-39 20.668008342061647 27.18371281135025 31.15534831695627 20.992930529631828 40-41 20.794183410597153 27.132867997934135 31.223345528543284 20.84960306292543 42-43 20.79826369726562 27.238867839545435 30.740923140886515 21.22194532230243 44-45 20.51388190220206 27.434569033312727 30.630398182368307 21.42115088211691 46-47 20.794664452390485 27.575287143317667 30.194303972517893 21.435744431773955 48-49 20.9056749491319 27.679053234518026 29.740572693113126 21.674699123236945 50-51 20.735013213852795 27.898647799929087 29.57762078872253 21.788718197495587 52-53 20.903780345175406 27.926472317279188 29.43075142791725 21.73899590962815 54-55 20.38891841771944 27.771648568827988 29.57336786865851 22.26606514479405 56-57 20.692846533076224 27.997881755692937 29.234431615786114 22.074840095444724 58-59 20.840057226022378 27.396803581512753 29.47505082737126 22.28808836509361 60-61 20.638497048781364 27.483926895352617 29.73589282865619 22.14168322720983 62-63 20.67147909020943 27.292789036187216 29.731212864365382 22.30451900923797 64-65 20.427639294690373 27.38189913238078 29.627410801206338 22.56305077172251 66-67 20.42052988944597 27.446277968845834 29.352736247896516 22.780455893811684 68-69 20.48901138691047 27.244035906802743 29.45537552912894 22.811577177157847 70-71 20.500960600523875 27.364426585857387 29.276405373421078 22.858207440197667 72-73 20.642107582355283 27.19036467294301 29.163933249329745 23.003594495371964 74-75 20.421175489168306 27.40140825023238 29.16988983270066 23.007526427898654 76-77 20.503658329079464 27.203118474175135 29.13985181060219 23.15337138614321 78-79 20.634746837400638 27.166125390492095 29.127215366057346 23.071912406049922 80-81 20.757818338389665 27.208287760384447 28.99021068312407 23.043683218101823 82-83 20.770011761525026 26.99130785559743 28.979382993243156 23.25929738963439 84-85 20.745373586988197 26.812129056367034 28.96337785224062 23.479119504404146 86-87 21.050202176139194 26.48739454201768 28.89661637270947 23.56578690913366 88-89 21.18956301337482 26.22049699818443 28.985187082849116 23.60475290559163 90-91 21.23557117211001 26.167348103188896 29.105064578151907 23.49201614654918 92-93 21.58280014926472 25.985428743113058 28.96601999726472 23.4657511103575 94-95 21.50019711097812 25.675546046919223 28.989609745018228 23.83464709708443 96-97 21.824825107312073 25.253612963595586 28.899624222080295 24.021937707012047 98-99 21.803734703033868 25.200241347834833 28.81136191345514 24.184662035676162 100-101 22.281424628104592 24.597846808979323 29.298879471918116 23.82184909099797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1567.0 1 1027.0 2 651.0 3 805.0 4 701.0 5 687.5 6 1018.0 7 1662.0 8 2455.5 9 2779.5 10 2352.5 11 1996.0 12 2022.5 13 2102.5 14 2197.5 15 2230.5 16 2287.0 17 2405.0 18 2547.0 19 2732.5 20 2923.5 21 3211.0 22 3608.0 23 4096.5 24 4651.0 25 5358.0 26 6332.5 27 7506.0 28 8477.5 29 9466.5 30 10882.5 31 12505.5 32 14270.5 33 15802.5 34 17430.0 35 18774.0 36 20158.5 37 21967.0 38 22557.5 39 22459.0 40 22887.5 41 23908.0 42 25009.0 43 25952.0 44 26980.5 45 28126.5 46 28639.0 47 28995.0 48 30220.5 49 30094.0 50 28177.5 51 26251.0 52 24814.5 53 24067.0 54 22876.5 55 21066.0 56 19054.0 57 16992.0 58 15431.5 59 13785.0 60 11992.0 61 10193.0 62 8649.5 63 6983.0 64 5314.5 65 4109.0 66 3040.0 67 2417.0 68 1854.0 69 1273.0 70 971.5 71 758.5 72 637.5 73 544.0 74 439.5 75 372.0 76 293.0 77 205.0 78 133.5 79 94.5 80 67.0 81 51.5 82 41.5 83 32.5 84 19.5 85 21.0 86 20.0 87 7.5 88 5.0 89 6.0 90 3.5 91 6.0 92 6.0 93 4.0 94 3.5 95 1.5 96 1.5 97 2.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15977117122000134 2 0.09263525116225212 3 0.08610643691810403 4 0.10014954680174332 5 0.11825776744117292 6 0.038803329941634865 7 0.041759774127664184 8 0.047426292150887055 9 0.04619444040670817 10-11 0.04502418124973823 12-13 0.04958203270320011 14-15 0.04662558851717078 16-17 0.04674877369158867 18-19 0.05075229186017004 20-21 0.051984143604348935 22-23 0.06473380915660037 24-25 0.08031673372046327 26-27 0.08247247427277632 28-29 0.0714474011623753 30-31 0.06159258720894423 32-33 0.05075229186017004 34-35 0.2878221600273964 36-37 0.4622523670031265 38-39 0.44254273909626424 40-41 0.4198766670033728 42-43 0.44740855348577085 44-45 0.45596992310781415 46-47 0.4553539972357247 48-49 0.4698282552298266 50-51 0.45978866351476866 52-53 0.4669949962182151 54-55 0.4588031821194256 56-57 0.4397094800846529 58-59 0.4203694077010444 60-61 0.3864318921489161 62-63 0.44346662790439845 64-65 0.4786359952007056 66-67 0.4804837728169739 68-69 0.4893531053750619 70-71 0.48756692034600246 72-73 0.47845121743907876 74-75 0.4721687735437664 76-77 0.45560036758456046 78-79 0.4325031473812063 80-81 0.43829285057884715 82-83 0.4487019978171587 84-85 0.44685422020089044 86-87 0.4562162934566499 88-89 0.46502403342752896 90-91 0.4695202922937819 92-93 0.4712448847356323 94-95 0.4761722917123478 96-97 0.47574114360188524 98-99 0.4709985143867965 100-101 0.45941910799151503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811786.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.44741561480907 #Duplication Level Percentage of deduplicated Percentage of total 1 83.12085159868509 51.906823660399546 2 10.085988401311873 12.596878191657323 3 2.773689373547698 5.196291992889375 4 1.2146691723988274 3.0341180257314297 5 0.6315168148728335 1.971829650305213 6 0.40373191236380634 1.512720871700657 7 0.25867224547327977 1.130738925476007 8 0.22033558167656578 1.100751011494977 9 0.1654663816621585 0.9299653115341888 >10 1.0152383092472195 11.795011184214394 >50 0.08104990357940392 3.444776397375978 >100 0.02681836647611325 2.75802203815324 >500 9.859693525737562E-4 0.4686802632269691 >1k 7.887754820590049E-4 1.3353570085216113 >5k 1.9719387051475123E-4 0.8180354673191685 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6643 0.818319113658033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4798 0.5910424668570289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3269 0.4026923351720774 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1664 0.2049801302313664 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1113 0.13710509912710986 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 869 0.10704791656914506 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 867 0.1068015462203093 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2318517441788846E-4 0.0 12-13 0.0 0.0 0.0 2.4637034883577693E-4 0.0 14-15 0.0 0.0 0.0 2.4637034883577693E-4 0.0 16-17 6.159258720894423E-5 0.0 0.0 2.4637034883577693E-4 0.0 18-19 1.2318517441788846E-4 0.0 0.0 2.4637034883577693E-4 0.0 20-21 1.2318517441788846E-4 0.0 0.0 5.54333284880498E-4 0.0 22-23 1.2318517441788846E-4 0.0 0.0 0.0018477776162683268 0.0 24-25 1.2318517441788846E-4 0.0 0.0 0.004557851453461873 0.0 26-27 1.2318517441788846E-4 0.0 0.0 0.009054110319714802 0.0 28-29 1.2318517441788846E-4 0.0 0.0 0.02944125668587534 0.0 30-31 1.2318517441788846E-4 0.0 0.0 0.08678395537740241 0.0 32-33 1.2318517441788846E-4 0.0 0.0 0.19715787165583049 0.0 34-35 1.2318517441788846E-4 0.0 0.0 0.36844685668390437 0.0 36-37 1.2318517441788846E-4 0.0 0.0 0.6213460197638294 0.0 38-39 1.2318517441788846E-4 0.0 0.0 1.0052526158371788 0.0 40-41 1.2318517441788846E-4 0.0 0.0 1.4454548366195032 0.0 42-43 1.2318517441788846E-4 0.0 0.0 1.8572628746985043 0.0 44-45 1.2318517441788846E-4 0.0 0.0 2.3098452055098266 0.0 46-47 1.2318517441788846E-4 0.0 0.0 2.8377552704776874 0.0 48-49 1.2318517441788846E-4 0.0 0.0 3.386914778032634 0.0 50-51 1.2318517441788846E-4 0.0 0.0 4.009369464366225 0.0 52-53 1.2318517441788846E-4 0.0 0.0 4.569369267269945 1.2318517441788846E-4 54-55 1.2318517441788846E-4 0.0 0.0 5.135220365958515 1.2318517441788846E-4 56-57 2.4637034883577693E-4 0.0 0.0 5.7916125678442345 1.2318517441788846E-4 58-59 2.4637034883577693E-4 0.0 0.0 6.501343950252899 1.2318517441788846E-4 60-61 2.4637034883577693E-4 0.0 0.0 7.279073548940238 1.2318517441788846E-4 62-63 2.4637034883577693E-4 0.0 0.0 8.041589778586967 1.2318517441788846E-4 64-65 2.4637034883577693E-4 0.0 0.0 8.792649787012834 1.2318517441788846E-4 66-67 2.4637034883577693E-4 0.0 0.0 9.61546515953712 1.2318517441788846E-4 68-69 2.4637034883577693E-4 0.0 0.0 10.51619515488072 1.2318517441788846E-4 70-71 2.4637034883577693E-4 0.0 0.0 11.514056660252825 1.2318517441788846E-4 72-73 2.4637034883577693E-4 0.0 0.0 12.498860537136634 1.2318517441788846E-4 74-75 2.4637034883577693E-4 0.0 0.0 13.478737007043728 1.2318517441788846E-4 76-77 2.4637034883577693E-4 0.0 0.0 14.48318891924719 1.2318517441788846E-4 78-79 2.4637034883577693E-4 0.0 0.0 15.579906527089651 1.2318517441788846E-4 80-81 2.4637034883577693E-4 0.0 0.0 16.71813753871094 1.2318517441788846E-4 82-83 2.4637034883577693E-4 1.2318517441788846E-4 0.0 17.881744696262317 1.2318517441788846E-4 84-85 2.4637034883577693E-4 1.2318517441788846E-4 0.0 19.034573151052122 1.2318517441788846E-4 86-87 2.4637034883577693E-4 1.2318517441788846E-4 0.0 20.16553623738276 1.2318517441788846E-4 88-89 2.4637034883577693E-4 2.4637034883577693E-4 0.0 21.35667528141653 1.2318517441788846E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 9.751371E-4 42.109028 2 GGTATCA 3520 0.0 41.466415 1 GTATCAA 5705 0.0 36.051434 1 ACCGCTT 65 0.0059327176 29.139826 8 TATCAAC 7070 0.0 28.879229 2 ATCAACG 7060 0.0 28.853033 3 TCAACGC 7130 0.0 28.636208 4 CAACGCA 7240 0.0 28.266558 5 AACGCAG 7360 0.0 27.725937 6 GTACATG 4315 0.0 26.578047 1 GTACTAT 90 9.066948E-4 26.327883 1 GTCTAGA 205 9.822543E-10 25.428883 1 TACATGG 4355 0.0 25.23641 2 ACATGGG 4350 0.0 24.93871 3 ACGCAGA 8230 0.0 24.51038 7 CGCAGAG 8280 0.0 24.247995 8 GAGTACT 4825 0.0 23.554161 12-13 GCAGAGT 8460 0.0 23.228333 9 CATGGGG 1340 0.0 22.625746 4 CATGGGA 2790 0.0 22.07328 4 >>END_MODULE