FastQCFastQC Report
Thu 26 May 2016
SRR938803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811786
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62180.7659654145304304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.6031146139499819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.42954670319517707No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT28570.3519400433119073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17130.21101620377784294No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14520.17886487325477404RNA PCR Primer, Index 26 (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.13611961773176676No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.10914206453424917No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.10162776889475798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.041.2644961
GTATCAA59000.036.7378961
ATCAACG74500.029.1708223
TATCAAC75000.029.0396162
TCAACGC75800.028.7331314
CAACGCA76800.028.2972165
AACGCAG78000.027.922716
TACGTAC856.4448314E-427.9119743
ACGCAGA87850.024.575887
CGCAGAG88500.024.3417638
GCAGAGT92250.023.1465159
GAGTACT51350.023.05523512-13
AGTACTT52500.022.59540712-13
GTACATG43200.022.4957921
GTGTTAC2154.28372E-822.1572781
TACATGG43750.021.5831322
AGAGTAC79400.021.03592510-11
TAGGGGT2056.8930603E-720.8318634
ACATGGG43600.020.6779063
GTACTTT57650.020.65921614-15
Produced by FastQC (version 0.11.5)