##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811786 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912427659506324 31.0 31.0 34.0 30.0 34.0 2 32.12341553069405 33.0 31.0 34.0 30.0 34.0 3 32.194940538516306 34.0 31.0 34.0 30.0 34.0 4 35.871142148300166 37.0 35.0 37.0 35.0 37.0 5 35.782995764893705 37.0 35.0 37.0 35.0 37.0 6 35.81469500582666 37.0 35.0 37.0 33.0 37.0 7 35.738534539891056 37.0 35.0 37.0 33.0 37.0 8 35.71813630685919 37.0 35.0 37.0 33.0 37.0 9 37.33324669309399 39.0 37.0 39.0 34.0 39.0 10-11 37.2695962975464 39.0 37.0 39.0 33.0 39.0 12-13 37.19987225697413 39.0 37.0 39.0 33.0 39.0 14-15 38.37045600687866 40.0 38.0 41.0 33.0 41.0 16-17 38.31232985047784 40.0 38.0 41.0 33.0 41.0 18-19 38.361314435085106 40.0 38.0 41.0 33.5 41.0 20-21 38.422703150830394 40.0 38.0 41.0 34.0 41.0 22-23 38.352426008824985 40.0 38.0 41.0 33.5 41.0 24-25 38.2538575437369 40.0 38.0 41.0 33.0 41.0 26-27 38.066338911979265 40.0 37.0 41.0 33.0 41.0 28-29 37.871856622311796 40.0 37.0 41.0 33.0 41.0 30-31 37.565605344265606 40.0 36.5 41.0 32.0 41.0 32-33 37.25000123185174 39.0 35.5 41.0 31.5 41.0 34-35 37.10901333602698 39.0 35.0 41.0 31.0 41.0 36-37 36.95935074021971 40.0 35.0 41.0 31.0 41.0 38-39 36.79331560288056 39.0 35.0 41.0 31.0 41.0 40-41 36.57027837385715 39.0 35.0 41.0 30.0 41.0 42-43 36.309030335581056 39.0 35.0 41.0 30.0 41.0 44-45 35.94503477517473 39.0 35.0 41.0 29.5 41.0 46-47 35.48851051878204 39.0 35.0 41.0 26.5 41.0 48-49 35.062818279694405 39.0 35.0 41.0 25.0 41.0 50-51 34.75951728657553 39.0 34.5 40.5 24.0 41.0 52-53 34.53074455583122 38.0 34.0 40.0 23.5 41.0 54-55 34.25873074923687 38.0 34.0 40.0 23.0 41.0 56-57 33.9650776189784 38.0 34.0 40.0 22.0 41.0 58-59 33.61944896807779 37.5 33.0 40.0 20.0 41.0 60-61 33.23609424158583 37.0 33.0 40.0 18.5 41.0 62-63 32.83063701517395 36.5 32.5 40.0 14.5 41.0 64-65 32.42845836217919 36.0 32.0 39.0 10.0 41.0 66-67 31.988864676158496 35.5 31.5 39.0 6.5 40.0 68-69 31.515979580825487 35.0 31.0 38.5 2.0 40.0 70-71 31.052565577627604 35.0 31.0 38.0 2.0 40.0 72-73 30.7393654238925 35.0 31.0 37.0 2.0 39.5 74-75 30.330759584422495 35.0 30.0 37.0 2.0 39.0 76-77 28.302885243155217 32.5 27.0 35.0 2.0 37.0 78-79 29.313050483748178 34.0 29.0 35.5 2.0 37.5 80-81 29.349101240967446 34.0 29.5 35.0 2.0 37.0 82-83 29.04351516286312 34.0 29.0 35.0 2.0 37.0 84-85 28.67270315083039 34.0 29.0 35.0 2.0 36.0 86-87 28.154788946840668 34.0 28.5 35.0 2.0 36.0 88-89 27.658172227656056 34.0 27.0 35.0 2.0 35.5 90-91 27.261212930501387 33.5 26.0 35.0 2.0 35.0 92-93 26.997475319850306 33.0 26.0 35.0 2.0 35.0 94-95 26.639178305612564 33.0 25.0 35.0 2.0 35.0 96-97 26.156652122603738 33.0 24.5 35.0 2.0 35.0 98-99 25.48087796537511 33.0 21.5 35.0 2.0 35.0 100-101 22.734252007302416 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.024955566746111657 1101 2 0.1477292926648559 1101 3 0.26650478271203326 1101 4 0.16198886068320917 1101 5 0.07519937822967648 1101 6 0.10859388176226759 1101 7 0.3344406138923972 1101 8 0.2433500267860964 1101 9 0.33330854537646815 1101 10-11 0.33612463128940107 1101 12-13 0.2416547641442861 1101 14-15 0.42903163554080237 1101 16-17 0.4905822721658595 1101 18-19 0.3364534660398135 1101 20-21 0.3276248600442173 1101 22-23 0.10219731975890056 1101 24-25 0.2710604201921143 1101 26-27 0.451490814791768 1101 28-29 0.3692019101451294 1101 30-31 0.39384016400926214 1101 32-33 0.33334207880434263 1101 34-35 0.12493189680072447 1101 36-37 0.3722468886641792 1101 38-39 0.03266812804459107 1101 40-41 0.29990570958343454 1101 42-43 0.2641833231533184 1101 44-45 0.35977381790480223 1101 46-47 0.4873308360910755 1101 48-49 0.5222070961449887 1101 50-51 0.5676813288121849 1101 52-53 0.5003174965196777 1101 54-55 0.3559937013826868 1101 56-57 0.23858772435782782 1101 58-59 0.23684146585540589 1101 60-61 0.381718177946766 1101 62-63 0.1922640273102516 1101 64-65 0.44538481340408254 1101 66-67 0.2104480899535055 1101 68-69 0.24342733762332358 1101 70-71 0.1339914547452281 1101 72-73 0.12156890329844217 1101 74-75 0.16273610324873644 1101 76-77 0.1177786416726434 1101 78-79 0.37373790768200976 1101 80-81 0.21658514415894814 1101 82-83 0.39238088574108687 1101 84-85 0.3659046121712848 1101 86-87 0.39664346471558076 1101 88-89 0.38090589453151225 1101 90-91 0.48598170304872923 1101 92-93 0.2863986877415954 1101 94-95 0.17045738564821988 1101 96-97 0.15030946848899873 1101 98-99 0.04721694664208442 1101 100-101 0.670959159569243 1102 1 -0.13144457052224823 1102 2 -0.05742970985172491 1102 3 0.061917731693959865 1102 4 -0.01680317775193174 1102 5 0.08988423295399883 1102 6 0.016195117177502993 1102 7 0.01303246930306301 1102 8 0.11594783049233826 1102 9 0.198428083239655 1102 10-11 0.2085079531836982 1102 12-13 0.15959402289514202 1102 14-15 0.34313881925162093 1102 16-17 0.2899130889126056 1102 18-19 0.27304549303592296 1102 20-21 0.2576680423823561 1102 22-23 0.2254671664759087 1102 24-25 0.15755664531222635 1102 26-27 0.18511155375311006 1102 28-29 0.1735516012913152 1102 30-31 0.21454766801291214 1102 32-33 0.16337954162749213 1102 34-35 0.20058678126651586 1102 36-37 0.09113272714508014 1102 38-39 0.13980894250352804 1102 40-41 0.2943792282290545 1102 42-43 0.15712830141916356 1102 44-45 0.17220947336122094 1102 46-47 0.12454200058432718 1102 48-49 0.020062278025569924 1102 50-51 0.30749344274492074 1102 52-53 0.41755611468485654 1102 54-55 0.48443970916123646 1102 56-57 0.268922601960206 1102 58-59 0.2835413057089866 1102 60-61 0.1724053776722343 1102 62-63 -0.046025265757691614 1102 64-65 0.22331034531897132 1102 66-67 0.5413935257805527 1102 68-69 0.4288826110161388 1102 70-71 0.21058290978983862 1102 72-73 0.47008754891729154 1102 74-75 0.24997300555942203 1102 76-77 0.1997821877719339 1102 78-79 -0.005902048849677044 1102 80-81 0.258251810825616 1102 82-83 0.3318612678046904 1102 84-85 0.412911875955313 1102 86-87 0.14946032584575875 1102 88-89 0.37454293411055417 1102 90-91 0.4044428675419809 1102 92-93 0.20182135989899308 1102 94-95 0.2580231600705467 1102 96-97 0.43291070390423414 1102 98-99 0.3461259491586688 1102 100-101 0.8168353892649662 1103 1 0.09965845299398879 1103 2 0.0877830561057209 1103 3 0.049795671223634486 1103 4 2.7408988524513234E-4 1103 5 0.09110203810517703 1103 6 0.05205737921557585 1103 7 0.11944344354718339 1103 8 0.14649094359424453 1103 9 0.15454510451625225 1103 10-11 0.13423583672233264 1103 12-13 0.2614977183038789 1103 14-15 0.23800164120010692 1103 16-17 -0.007189374693219008 1103 18-19 -0.006790733167882479 1103 20-21 0.12273663140810953 1103 22-23 0.2231155427356981 1103 24-25 -0.07526810272808859 1103 26-27 0.26315606663104774 1103 28-29 0.20044129893969398 1103 30-31 0.1752139614059729 1103 32-33 0.33515305786490046 1103 34-35 0.13583314184882767 1103 36-37 0.2383051795526967 1103 38-39 0.5874573187633203 1103 40-41 0.39949540964003205 1103 42-43 0.4595639117215171 1103 44-45 0.47214648343960164 1103 46-47 0.5680750352987758 1103 48-49 0.7107257718665494 1103 50-51 0.7508095121738094 1103 52-53 0.5319318145728715 1103 54-55 0.5042605378286424 1103 56-57 0.638092534770955 1103 58-59 0.2095211489340727 1103 60-61 0.14184126792979157 1103 62-63 0.23649153155473357 1103 64-65 0.3565690239303976 1103 66-67 0.40024011032702234 1103 68-69 0.46503882602173974 1103 70-71 0.2520092815703556 1103 72-73 0.461926854628377 1103 74-75 0.4551101836109339 1103 76-77 0.20111197500597555 1103 78-79 0.20205567791403922 1103 80-81 0.4108833897729838 1103 82-83 0.27129995761432113 1103 84-85 0.22274530260705205 1103 86-87 0.28182314779424544 1103 88-89 0.3492349833826687 1103 90-91 0.3621206390985314 1103 92-93 0.2788930284320301 1103 94-95 -0.036517713389699225 1103 96-97 -0.061490191952429996 1103 98-99 0.11440002530648385 1103 100-101 0.6227984351776179 1104 1 0.09152448170211969 1104 2 0.14589993922260192 1104 3 0.1814258831170008 1104 4 0.11332945557219176 1104 5 0.2016796184195968 1104 6 0.11207104975556348 1104 7 0.032789308208833745 1104 8 -0.006482398852782012 1104 9 0.11038926036040664 1104 10-11 0.17619585039287244 1104 12-13 0.22015287893272983 1104 14-15 0.2103017095528088 1104 16-17 0.19667255285292384 1104 18-19 0.2105195880898023 1104 20-21 0.23301516866033012 1104 22-23 0.11342654752039039 1104 24-25 0.144238049014902 1104 26-27 0.08067144598852849 1104 28-29 0.13294300775719847 1104 30-31 0.05950138450918985 1104 32-33 -0.15579875348164762 1104 34-35 -0.019677794583166985 1104 36-37 -0.0016726058198273108 1104 38-39 0.13069040147008337 1104 40-41 0.2911307479858962 1104 42-43 0.041108682739974256 1104 44-45 -0.021761582178982053 1104 46-47 -0.14891061063423905 1104 48-49 -0.41804217071717886 1104 50-51 -0.1377072342376735 1104 52-53 -0.17860304530409365 1104 54-55 -0.38095819081114257 1104 56-57 -0.2743818329665686 1104 58-59 -0.5435034580379039 1104 60-61 -0.16170452837916116 1104 62-63 -0.17933211847943653 1104 64-65 -0.12636843334916392 1104 66-67 -0.11460951373313222 1104 68-69 -0.19244579462077738 1104 70-71 -0.32907410872356024 1104 72-73 -0.5037173695684807 1104 74-75 -0.4569626120144932 1104 76-77 -0.48200490811090546 1104 78-79 -0.2721582660367474 1104 80-81 -0.2771377995639952 1104 82-83 -0.23867099248820978 1104 84-85 -0.13473077394797528 1104 86-87 -0.06730535528162562 1104 88-89 0.1933395630135628 1104 90-91 0.11272726931042598 1104 92-93 0.2177857146931359 1104 94-95 0.22112070082766522 1104 96-97 0.2852801429758003 1104 98-99 0.5852475987856387 1104 100-101 1.170662413304754 1105 1 0.0017314103233729838 1105 2 -0.14616835054389554 1105 3 -0.1008672712989167 1105 4 -0.0236187624501909 1105 5 -0.13329895799120806 1105 6 -0.04939638306736782 1105 7 -0.055994031213892015 1105 8 -0.1557300821158094 1105 9 -0.05133724842492171 1105 10-11 0.10774000955851903 1105 12-13 -0.046223711229025355 1105 14-15 -0.02085217492588498 1105 16-17 -0.009309444689186819 1105 18-19 -0.10023870732322138 1105 20-21 0.030296531798470028 1105 22-23 0.006218921386931697 1105 24-25 0.06430148941082336 1105 26-27 0.008387591735363742 1105 28-29 0.054479527503438874 1105 30-31 -0.12173885862364386 1105 32-33 0.3098686992701971 1105 34-35 0.2911585557672609 1105 36-37 0.11231749614981368 1105 38-39 0.2917243802789997 1105 40-41 0.3184599405324775 1105 42-43 -0.015580791468686073 1105 44-45 0.1953175024192788 1105 46-47 0.2672135465385068 1105 48-49 0.3343409959882351 1105 50-51 0.290007250765818 1105 52-53 0.1299547651718811 1105 54-55 0.15610129701560993 1105 56-57 0.28651762563109884 1105 58-59 0.36255991592828707 1105 60-61 0.6901704508615438 1105 62-63 0.566408074831088 1105 64-65 0.49877356020714814 1105 66-67 0.7913459119153892 1105 68-69 0.8486974611004889 1105 70-71 0.5257237788108995 1105 72-73 0.42867709019170874 1105 74-75 0.6141238462842402 1105 76-77 0.39612476272975883 1105 78-79 0.3851523261843326 1105 80-81 0.4964231663243375 1105 82-83 0.4944575158321136 1105 84-85 0.5600030088932293 1105 86-87 0.9199002779598118 1105 88-89 0.9264896609974222 1105 90-91 0.7856853853631698 1105 92-93 0.9903817886958599 1105 94-95 0.9853997721399814 1105 96-97 0.9357335259100026 1105 98-99 0.7766964312830602 1105 100-101 0.9680434210438165 1106 1 -0.2621329148858642 1106 2 -0.0793763269277008 1106 3 -0.042441509130853206 1106 4 -0.051275091551637786 1106 5 0.014059012804132465 1106 6 -0.06596453933401136 1106 7 -0.058837573906480145 1106 8 -0.17075976672911963 1106 9 -0.08195875152487986 1106 10-11 -0.18587138828105054 1106 12-13 -0.01382869219537497 1106 14-15 0.03891153160736138 1106 16-17 -0.17550218757374836 1106 18-19 -0.26528417113297564 1106 20-21 0.016626159212194125 1106 22-23 -0.133862538714709 1106 24-25 -0.029721153736076644 1106 26-27 -0.06309545549045481 1106 28-29 -0.11929216067334636 1106 30-31 0.06316045037904416 1106 32-33 -0.036773947952987385 1106 34-35 -0.15650382202615987 1106 36-37 -0.047824911098096834 1106 38-39 -0.0938780891652371 1106 40-41 -0.038028267805508165 1106 42-43 -0.03565324427903249 1106 44-45 0.1588346853067435 1106 46-47 0.054524771464379285 1106 48-49 -0.29476570425305937 1106 50-51 -0.308681760996258 1106 52-53 -0.14943234453955512 1106 54-55 -0.1399723129357966 1106 56-57 -0.3419710560827838 1106 58-59 -0.3701602202987786 1106 60-61 -0.2768148679110993 1106 62-63 -0.18861332570409672 1106 64-65 -0.3017231557475881 1106 66-67 -0.4025639757235844 1106 68-69 -0.42708673208947445 1106 70-71 -0.37303130833629083 1106 72-73 -0.16427223886370612 1106 74-75 -0.08477785589657216 1106 76-77 -0.29567465738476884 1106 78-79 -0.2957084947283626 1106 80-81 -0.4560798087116602 1106 82-83 -0.12294134938067458 1106 84-85 -0.5436580934151394 1106 86-87 -0.3695805864771664 1106 88-89 -0.18813140817463037 1106 90-91 -0.3780582947399189 1106 92-93 -0.1698148595398301 1106 94-95 -0.07265287777881824 1106 96-97 -0.35040576494661124 1106 98-99 -0.542676148418316 1106 100-101 0.1387874759028236 1107 1 -0.1572337775824515 1107 2 -0.1973673198341288 1107 3 -0.11317425358839728 1107 4 -0.176417639438057 1107 5 -0.16535159598004157 1107 6 -0.08503535261399975 1107 7 -0.036132997555576196 1107 8 -0.19486243986440144 1107 9 -0.06350000826570579 1107 10-11 -0.16516892518242798 1107 12-13 -0.2991225824479997 1107 14-15 0.012375074784067408 1107 16-17 -0.06608661780099112 1107 18-19 -0.011740607854598295 1107 20-21 -0.03356036107309279 1107 22-23 -0.10539322919412086 1107 24-25 -0.3096352706478882 1107 26-27 -0.14141786820604807 1107 28-29 -0.01534817474450989 1107 30-31 -0.2619727553860045 1107 32-33 -0.13843716769900993 1107 34-35 -0.11219317394064632 1107 36-37 -0.021939181349566184 1107 38-39 0.14322172979088776 1107 40-41 0.01569215149466885 1107 42-43 0.08775940044331776 1107 44-45 0.12957129546968105 1107 46-47 0.3184509751484015 1107 48-49 0.2651430650996289 1107 50-51 0.228127807913161 1107 52-53 0.19317868174079678 1107 54-55 -0.033270790492153424 1107 56-57 -0.011280898813005535 1107 58-59 0.025749219109513888 1107 60-61 0.11655931304257194 1107 62-63 -0.3300498218287231 1107 64-65 0.08103644247676556 1107 66-67 0.026869183008724207 1107 68-69 0.02193105659818073 1107 70-71 -0.054569665798066325 1107 72-73 -0.05533505013352524 1107 74-75 0.19743474501444425 1107 76-77 -0.11279779943011548 1107 78-79 -0.08309469802580693 1107 80-81 0.0912718609509291 1107 82-83 0.06569218819076283 1107 84-85 -0.20742387032527887 1107 86-87 -0.3258836191230472 1107 88-89 -0.12651689631657703 1107 90-91 0.03138755639176338 1107 92-93 0.18904340437187983 1107 94-95 0.20364519387847224 1107 96-97 0.17441206368666684 1107 98-99 0.30475090249946746 1107 100-101 0.26208414946333036 1108 1 -0.0415137659428666 1108 2 -0.07854028971200933 1108 3 -0.014927700877031214 1108 4 -0.059256759710649476 1108 5 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-0.6061754728765223 2316 54-55 -0.8313861412390864 2316 56-57 -0.7692113277960644 2316 58-59 -0.6758683904028402 2316 60-61 -0.6641372308118605 2316 62-63 -0.8303702695175943 2316 64-65 -0.9374114443921755 2316 66-67 -0.8019127510363653 2316 68-69 -1.0081584603334406 2316 70-71 -0.914072893722345 2316 72-73 -0.9963166621677715 2316 74-75 -0.9799004849523634 2316 76-77 -0.8804888565948552 2316 78-79 -0.8649128087912885 2316 80-81 -0.6073926798188758 2316 82-83 -0.8260889799061957 2316 84-85 -1.0775487667659647 2316 86-87 -1.115208803185073 2316 88-89 -1.478516958842956 2316 90-91 -2.0779671713840173 2316 92-93 -2.2279293810219585 2316 94-95 -2.2615614818545176 2316 96-97 -2.076494568798914 2316 98-99 -2.7802328666948277 2316 100-101 -3.42202867938634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 10.0 5 37.0 6 119.0 7 262.0 8 570.0 9 940.0 10 1674.0 11 2467.0 12 3538.0 13 5005.0 14 5313.0 15 7190.0 16 8862.0 17 11965.0 18 10909.0 19 6902.0 20 6251.0 21 6566.0 22 6840.0 23 7295.0 24 8238.0 25 9362.0 26 10539.0 27 12271.0 28 14676.0 29 17498.0 30 21015.0 31 25280.0 32 30663.0 33 38446.0 34 50544.0 35 70163.0 36 101356.0 37 154406.0 38 139769.0 39 14838.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.05998446843977 20.818968288906806 14.953578441798692 22.16746880085473 2 15.840012022873024 22.525394623706248 38.875146898320494 22.759446455100235 3 17.983926798441953 27.149396515830524 32.0143486091162 22.85232807661132 4 12.663928670856606 18.57225919146179 37.26191385414383 31.501898283537777 5 12.438869357195124 38.556466852101416 34.372359217823416 14.632304572880045 6 29.106932122505192 37.758719662571174 17.945985764721247 15.188362450202394 7 25.632617463223067 35.22874255037658 21.742552840280567 17.39608714611979 8 25.540967693456157 34.29118018788203 21.151633558597954 19.016218560063862 9 26.25556489025433 16.3652736065909 21.481277085340224 35.897884417814545 10-11 23.739630887943374 27.429150046933554 28.096444136755256 20.734774928367823 12-13 25.221918091713825 25.576075468165254 28.423500774834746 20.778505665286172 14-15 22.165365315548648 26.626709428425894 27.630485676925343 23.57743957910012 16-17 20.55008327502439 29.90238590336155 28.081915029910025 21.465615791704035 18-19 20.36265760022666 27.561669653910826 31.73303276113749 20.342639984725018 20-21 21.31183587792842 26.80620261990229 31.51243061595051 20.369530886218783 22-23 21.063248195953122 27.362383682399056 31.201634420894177 20.37273370075365 24-25 20.81774014333827 27.330786685160867 31.683411638042536 20.168061533458324 26-27 20.56846402491303 27.39620391631271 31.707141308525422 20.328190750248833 28-29 20.088915058894834 27.554429369316548 31.83597647655909 20.52067909522953 30-31 20.54352994508405 27.15580214490026 31.490072506793666 20.81059540322203 32-33 20.488958912816923 27.48944918981111 31.478616285572798 20.542975611799168 34-35 20.881079139925625 27.65231381984316 31.157646012389733 20.30896102784148 36-37 20.933712395390884 27.18534101724568 30.967747897037217 20.91319869032622 38-39 20.515118643483124 27.1358814532279 31.288418762431526 21.06058114085745 40-41 20.66026055622286 27.132772798669503 31.302411530998796 20.90455511410884 42-43 20.825518489842707 27.116690781040866 30.896334060768222 21.161456668348205 44-45 20.39063804049031 27.45319051041884 30.757125285408694 21.399046163682158 46-47 20.88227703629518 27.34145997441408 30.09265504151707 21.683607947773673 48-49 20.91099132037261 27.53410381553759 29.73480695651317 21.820097907576628 50-51 20.5464264794739 27.76282099845587 29.681682369745133 22.009070152325098 52-53 20.77203844362923 27.929220262482968 29.628251780641694 21.670489513246103 54-55 20.283054893777425 27.74826124126309 29.46330683209614 22.50537703286334 56-57 20.647621417467164 28.13962054038872 28.819602703175466 22.393155338968647 58-59 20.470826925126065 27.773935077597567 29.36604480622628 22.38919319105009 60-61 20.528160339008107 28.16699105669024 29.089839611717462 22.2150089925842 62-63 20.266671265579845 28.180456425708257 29.1035445302087 22.4493277785032 64-65 19.876543818198392 28.171402315388537 28.997543687622105 22.95451017879096 66-67 19.953263026668655 28.153051884938847 28.762024546149988 23.131660542242514 68-69 20.063391403110685 27.729612137248672 28.817958515956256 23.389037943684386 70-71 20.018022324029808 27.68440853393383 28.609607882025845 23.68796126001052 72-73 20.127410425900422 27.575863589665257 28.387777074253563 23.90894891018076 74-75 19.81883156398361 27.625445622368456 28.28528700913788 24.270435804510054 76-77 19.679109041766132 27.372649791859843 28.635424270523014 24.312816895851007 78-79 19.82930081356918 27.2956033745499 28.366539903337827 24.50855590854309 80-81 19.783107863402424 27.296479614085484 28.2082962751267 24.712116247385392 82-83 19.70938153651332 27.16085273705139 28.192035832103535 24.937729894331756 84-85 19.55484573520608 26.786554584582632 28.140113281086393 25.51848639912489 86-87 19.679343363902905 26.58035137630921 28.08031035505897 25.659994904728915 88-89 19.714367108280587 26.364282973197273 28.071764785126476 25.849585133395664 90-91 19.530579243397362 26.26039701687612 28.152335985630405 26.056687754096114 92-93 19.560142697706045 26.02102031816267 28.127363615534147 26.29147336859714 94-95 19.33939486514919 25.524768843020208 28.33308285681202 26.802753435018587 96-97 19.42272963564289 25.274025420492592 28.297543933992458 27.00570100987206 98-99 19.210898465601687 25.431272358164243 28.198510933943023 27.159318242291054 100-101 19.220592202596016 25.068275635237498 28.600192415953522 27.110939746212964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1146.0 1 794.5 2 573.5 3 692.0 4 626.0 5 649.5 6 943.0 7 1579.0 8 2258.0 9 2516.5 10 2136.0 11 1716.0 12 1707.5 13 1779.0 14 1844.5 15 1872.0 16 1964.5 17 2114.5 18 2248.5 19 2461.5 20 2680.5 21 2914.0 22 3291.5 23 3723.5 24 4220.5 25 4928.5 26 6022.5 27 7303.0 28 8105.0 29 9127.0 30 10768.5 31 12389.0 32 14200.0 33 15753.5 34 17355.0 35 18712.5 36 20020.5 37 21779.0 38 22330.0 39 22041.5 40 22637.5 41 24051.0 42 25157.5 43 26408.0 44 27655.0 45 28891.5 46 29545.0 47 29754.5 48 30952.5 49 30788.5 50 29049.5 51 27396.5 52 26015.0 53 25051.0 54 23678.5 55 21661.5 56 19425.5 57 17274.0 58 15727.5 59 14125.0 60 12113.5 61 10106.5 62 8472.0 63 6787.0 64 5060.0 65 3861.5 66 2832.5 67 2199.5 68 1641.0 69 1075.5 70 805.0 71 631.5 72 518.0 73 464.0 74 380.0 75 292.0 76 203.0 77 119.0 78 83.0 79 56.5 80 35.0 81 26.5 82 19.0 83 11.5 84 8.0 85 8.5 86 6.5 87 2.5 88 2.0 89 1.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3831058924396331 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8477776162683268E-4 16-17 0.002217333139521992 18-19 1.2318517441788846E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.4637034883577693E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008068628924371695 36-37 0.01644522078478811 38-39 0.011025073110401018 40-41 0.007514295639491196 42-43 0.00363396264532771 44-45 0.0014166295058057174 46-47 0.0014166295058057174 48-49 0.0 50-51 3.079629360447211E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.8477776162683268E-4 60-61 7.391110465073307E-4 62-63 0.0 64-65 0.0 66-67 0.001108666569760996 68-69 4.927406976715539E-4 70-71 0.0018477776162683268 72-73 0.0 74-75 0.0 76-77 0.09780902848780344 78-79 0.036955552325366535 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15157935712121173 88-89 0.3289660082829711 90-91 0.2832027159867256 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.4637034883577693E-4 100-101 3.6955552325366537E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811786.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.62431582503914 #Duplication Level Percentage of deduplicated Percentage of total 1 82.35710803947023 49.92843328221714 2 10.519848133870337 12.755171913984078 3 2.9293126520629995 5.327627261068508 4 1.2298769530201898 2.9824179530333126 5 0.6484805461208032 1.9656844717210717 6 0.43060214950022807 1.5662976423745527 7 0.3067306474304331 1.3016734950128916 8 0.21901140023190482 1.062193303755444 9 0.17076815381260377 0.9317432240634739 >10 1.0680819849836145 12.181862682175764 >50 0.08526321221757986 3.5391576007749483 >100 0.03207411692635327 3.1725650649205788 >500 0.0014210051763542226 0.6111547416245122 >1k 0.001218004436875048 1.9087817242440968 >5k 2.0300073947917467E-4 0.765235639029645 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6218 0.7659654145304304 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4896 0.6031146139499819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3487 0.42954670319517707 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2857 0.3519400433119073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1713 0.21101620377784294 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1452 0.17886487325477404 RNA PCR Primer, Index 26 (100% over 22bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.13611961773176676 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.10914206453424917 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.10162776889475798 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2318517441788846E-4 0.0 12-13 0.0 0.0 0.0 3.079629360447212E-4 0.0 14-15 0.0 0.0 0.0 3.6955552325366537E-4 0.0 16-17 0.0 0.0 0.0 3.6955552325366537E-4 0.0 18-19 6.159258720894423E-5 0.0 0.0 3.6955552325366537E-4 0.0 20-21 1.2318517441788846E-4 0.0 0.0 6.775184592983865E-4 0.0 22-23 1.2318517441788846E-4 0.0 0.0 0.0018477776162683268 0.0 24-25 1.2318517441788846E-4 0.0 0.0 0.004804221802297649 0.0 26-27 1.2318517441788846E-4 0.0 0.0 0.009115702906923746 0.0 28-29 1.8477776162683268E-4 0.0 0.0 0.029626034447502177 0.0 30-31 2.4637034883577693E-4 0.0 0.0 0.08770784418553658 0.0 32-33 2.4637034883577693E-4 0.0 0.0 0.2001143158418598 0.0 34-35 2.4637034883577693E-4 0.0 0.0 0.37318948589899303 0.0 36-37 2.4637034883577693E-4 0.0 0.0 0.6307696856067979 0.0 38-39 2.4637034883577693E-4 1.2318517441788846E-4 0.0 1.020527577464997 0.0 40-41 2.4637034883577693E-4 1.2318517441788846E-4 0.0 1.4663347236833353 0.0 42-43 2.4637034883577693E-4 1.2318517441788846E-4 0.0 1.882946983564634 0.0 44-45 2.4637034883577693E-4 1.2318517441788846E-4 0.0 2.3416269805096417 0.0 46-47 2.4637034883577693E-4 1.2318517441788846E-4 0.0 2.874464452454218 0.0 48-49 2.4637034883577693E-4 1.2318517441788846E-4 0.0 3.427812255939373 0.0 50-51 3.6955552325366537E-4 1.2318517441788846E-4 0.0 4.054640015964798 0.0 52-53 3.6955552325366537E-4 1.2318517441788846E-4 0.0 4.620799077589414 0.0 54-55 3.6955552325366537E-4 1.2318517441788846E-4 0.0 5.190284138923312 0.0 56-57 3.6955552325366537E-4 1.2318517441788846E-4 0.0 5.853697895750851 0.0 58-59 3.6955552325366537E-4 1.2318517441788846E-4 0.0 6.571189944147841 0.0 60-61 3.6955552325366537E-4 1.2318517441788846E-4 0.0 7.357973653154896 0.0 62-63 4.311481104626096E-4 1.2318517441788846E-4 0.0 8.127265067394609 0.0 64-65 4.927406976715539E-4 1.2318517441788846E-4 0.0 8.886270519570427 0.0 66-67 4.927406976715539E-4 1.2318517441788846E-4 0.0 9.717647261716756 0.0 68-69 4.927406976715539E-4 1.2318517441788846E-4 0.0 10.62755455255449 0.0 70-71 4.927406976715539E-4 1.2318517441788846E-4 0.0 11.633669464612595 0.0 72-73 4.927406976715539E-4 1.2318517441788846E-4 0.0 12.631284599635865 0.0 74-75 4.927406976715539E-4 1.2318517441788846E-4 0.0 13.624218698031255 0.0 76-77 4.927406976715539E-4 1.2318517441788846E-4 0.0 14.639018164885819 0.0 78-79 4.927406976715539E-4 1.2318517441788846E-4 0.0 15.742449364734055 0.0 80-81 4.927406976715539E-4 1.2318517441788846E-4 0.0 16.888933783041345 0.0 82-83 4.927406976715539E-4 2.4637034883577693E-4 0.0 18.063935569226373 0.0 84-85 4.927406976715539E-4 2.4637034883577693E-4 0.0 19.221691430992898 0.0 86-87 4.927406976715539E-4 2.4637034883577693E-4 0.0 20.36096951659674 0.0 88-89 4.927406976715539E-4 2.4637034883577693E-4 0.0 21.56066993025255 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3625 0.0 41.264496 1 GTATCAA 5900 0.0 36.737896 1 ATCAACG 7450 0.0 29.170822 3 TATCAAC 7500 0.0 29.039616 2 TCAACGC 7580 0.0 28.733131 4 CAACGCA 7680 0.0 28.297216 5 AACGCAG 7800 0.0 27.92271 6 TACGTAC 85 6.4448314E-4 27.911974 3 ACGCAGA 8785 0.0 24.57588 7 CGCAGAG 8850 0.0 24.341763 8 GCAGAGT 9225 0.0 23.146515 9 GAGTACT 5135 0.0 23.055235 12-13 AGTACTT 5250 0.0 22.595407 12-13 GTACATG 4320 0.0 22.495792 1 GTGTTAC 215 4.28372E-8 22.157278 1 TACATGG 4375 0.0 21.583132 2 AGAGTAC 7940 0.0 21.035925 10-11 TAGGGGT 205 6.8930603E-7 20.831863 4 ACATGGG 4360 0.0 20.677906 3 GTACTTT 5765 0.0 20.659216 14-15 >>END_MODULE