FastQCFastQC Report
Thu 26 May 2016
SRR938802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811158
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65240.8042822729973692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.5837333787005737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.42149618200153355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16740.20637163166732003No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.13832077104583818No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.11206201504515767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.039.9994471
GTATCAA57500.037.3090021
TATCAAC71450.029.813982
ATCAACG71350.029.789273
TCAACGC72000.029.4544474
CAACGCA72200.029.2414345
AACGCAG73300.028.8673386
GTACATG40800.027.1644341
TACATGG41050.026.0043032
ACATGGG41100.025.7417983
ACGCAGA81600.025.5822287
CGCAGAG82200.025.2800628
TAAGGTG2451.0913936E-1125.174055
GAGTACT47300.024.0352412-13
CATGGGA25200.023.909974
GCAGAGT86000.023.7768699
CATGGGG14300.023.2240434
AGTACTT48950.022.40079112-13
AGAGTAC71900.021.32246610-11
GTACTTT53250.021.3050414-15
Produced by FastQC (version 0.11.5)