##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938802_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811158 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.482044188678408 33.0 31.0 34.0 30.0 34.0 2 31.525583178616248 33.0 31.0 34.0 30.0 34.0 3 31.52554003042564 33.0 31.0 34.0 28.0 34.0 4 35.10390577421415 37.0 35.0 37.0 33.0 37.0 5 35.13656402328523 37.0 35.0 37.0 33.0 37.0 6 35.205699752699225 37.0 35.0 37.0 33.0 37.0 7 35.116384230938976 37.0 35.0 37.0 32.0 37.0 8 35.0971463019535 37.0 35.0 37.0 32.0 37.0 9 36.65500309434167 39.0 37.0 39.0 32.0 39.0 10-11 36.544262646734666 39.0 37.0 39.0 32.0 39.0 12-13 36.43737101773021 39.0 37.0 39.0 32.0 39.0 14-15 37.562934718020415 40.0 37.0 41.0 32.0 41.0 16-17 37.46292337621031 40.0 37.0 41.0 32.0 41.0 18-19 37.45606158109764 40.0 37.0 41.0 32.0 41.0 20-21 37.372616062468715 40.0 37.0 41.0 32.0 41.0 22-23 37.31673175879422 40.0 37.0 41.0 32.0 41.0 24-25 37.21691458384187 40.0 37.0 41.0 31.0 41.0 26-27 37.06811496650467 40.0 37.0 41.0 31.0 41.0 28-29 36.87403009031533 40.0 36.0 41.0 30.5 41.0 30-31 36.58029693351973 39.0 36.0 41.0 30.0 41.0 32-33 36.31126734865464 39.0 35.0 41.0 30.0 41.0 34-35 35.97333318539668 39.0 35.0 41.0 29.5 41.0 36-37 35.714464999420585 39.0 35.0 41.0 27.5 41.0 38-39 35.50675762305248 38.0 35.0 40.0 27.0 41.0 40-41 35.32102808577367 38.5 35.0 40.5 26.5 41.0 42-43 35.21752297332949 39.0 35.0 41.0 25.5 41.0 44-45 34.925708678210654 39.0 35.0 41.0 24.0 41.0 46-47 34.47529951008312 38.5 34.5 41.0 22.0 41.0 48-49 34.05982804829638 38.0 34.0 40.5 18.0 41.0 50-51 32.87625776975632 37.0 32.5 39.5 14.0 40.5 52-53 32.91298304399389 37.5 33.0 39.5 12.0 40.5 54-55 33.36594720140836 38.0 33.0 40.0 9.0 41.0 56-57 33.24783149522041 38.0 33.0 40.0 7.5 41.0 58-59 33.02863129008159 38.0 33.0 40.0 4.5 41.0 60-61 32.728165782745165 37.0 33.0 40.0 2.0 41.0 62-63 32.335228155303895 36.5 32.0 40.0 2.0 41.0 64-65 31.938738815372588 36.0 31.5 39.5 2.0 41.0 66-67 31.551439423638797 35.5 31.0 39.0 2.0 41.0 68-69 31.113777463823325 35.0 31.0 39.0 2.0 40.5 70-71 30.677144896555298 35.0 30.5 38.0 2.0 40.0 72-73 30.236427428441807 35.0 30.0 37.5 2.0 40.0 74-75 29.741883825345987 35.0 29.0 37.0 2.0 39.0 76-77 29.245925578000833 34.5 29.0 36.0 2.0 39.0 78-79 28.757596547158506 34.0 29.0 36.0 2.0 38.5 80-81 28.23487470998252 34.0 27.5 35.0 2.0 37.0 82-83 27.764456493063 34.0 26.5 35.0 2.0 37.0 84-85 27.365003488839413 34.0 26.0 35.0 2.0 36.5 86-87 26.86751286432483 34.0 25.0 35.0 2.0 36.0 88-89 26.352701323293367 33.5 24.5 35.0 2.0 36.0 90-91 25.83977104337256 33.0 21.5 35.0 2.0 35.0 92-93 25.31549772054273 33.0 18.5 35.0 2.0 35.0 94-95 24.741239068097705 33.0 6.5 35.0 2.0 35.0 96-97 24.11360733667177 32.0 2.0 35.0 2.0 35.0 98-99 23.330345012932128 32.0 2.0 35.0 2.0 35.0 100-101 21.121064576814874 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06705078519936336 1101 2 0.2785964496824249 1101 3 0.3676768022255139 1101 4 0.08085601490927274 1101 5 0.2518201903879742 1101 6 0.299059543039192 1101 7 0.29291224752901 1101 8 0.3337178562282901 1101 9 0.31259136367761187 1101 10-11 0.30806069372822975 1101 12-13 0.17202993732315974 1101 14-15 0.39369692231318254 1101 16-17 0.51888483643895 1101 18-19 0.6633585029907181 1101 20-21 0.8219513966496024 1101 22-23 0.8065850838765414 1101 24-25 0.7201015753327624 1101 26-27 0.4996628065684945 1101 28-29 0.7700805077613282 1101 30-31 0.7115747997186901 1101 32-33 0.5717991300141918 1101 34-35 0.6067129869733989 1101 36-37 0.5480405624336129 1101 38-39 0.693930508723227 1101 40-41 0.5970035754716605 1101 42-43 0.7013804469979661 1101 44-45 0.7444054225353227 1101 46-47 0.9047432107674211 1101 48-49 0.93240395453725 1101 50-51 0.9181176365565804 1101 52-53 0.7381356898000107 1101 54-55 0.7342639570050338 1101 56-57 0.8373653804515655 1101 58-59 0.8580959492420916 1101 60-61 0.6874548217317553 1101 62-63 0.6697851243811428 1101 64-65 0.8126960220421644 1101 66-67 0.6971398608696013 1101 68-69 0.5457806665962686 1101 70-71 0.43957902560574524 1101 72-73 0.44866110730358244 1101 74-75 0.3123441409066423 1101 76-77 0.35706863528723076 1101 78-79 0.2986273788453495 1101 80-81 0.19122373598712983 1101 82-83 0.18527999243007187 1101 84-85 0.2581934151575105 1101 86-87 0.04232564949125717 1101 88-89 0.04946273202373064 1101 90-91 0.17952866269102685 1101 92-93 0.17446088717898434 1101 94-95 0.19019489813948098 1101 96-97 0.1486132392793884 1101 98-99 0.20109372270472647 1101 100-101 0.2881206093092281 1102 1 0.34222506195084534 1102 2 0.43229111681382903 1102 3 0.4865509744109424 1102 4 0.6096552480915705 1102 5 0.6175748087218906 1102 6 0.5982317284696563 1102 7 0.6058522963265034 1102 8 0.6373166711463796 1102 9 0.6573194919453016 1102 10-11 0.7838144406783911 1102 12-13 0.7730450994005409 1102 14-15 0.9949386441674832 1102 16-17 1.1370052616742257 1102 18-19 1.075470737218673 1102 20-21 0.9868128466321835 1102 22-23 0.8587940417015645 1102 24-25 1.0405660228754599 1102 26-27 0.8732642356378619 1102 28-29 0.9115496607763092 1102 30-31 1.1461992333192441 1102 32-33 1.0827959930326685 1102 34-35 1.0340875077123357 1102 36-37 1.2334230999031064 1102 38-39 1.3408763086535203 1102 40-41 1.4511382914005537 1102 42-43 1.3632835491241408 1102 44-45 1.182306259063722 1102 46-47 1.2676832595649117 1102 48-49 1.1372662759035777 1102 50-51 1.2178126522414914 1102 52-53 0.9459345744288044 1102 54-55 1.0629194118673482 1102 56-57 1.0766691448294026 1102 58-59 1.0065710276666309 1102 60-61 0.7996411235790859 1102 62-63 0.7977173307248222 1102 64-65 0.7416564612195735 1102 66-67 0.5998803627866529 1102 68-69 0.8280654348081917 1102 70-71 0.7966023786904444 1102 72-73 0.5539460497922484 1102 74-75 0.6257285954204086 1102 76-77 0.8853014690272083 1102 78-79 0.864643412306485 1102 80-81 0.6819478558895362 1102 82-83 0.7877416515447457 1102 84-85 0.7133825054328646 1102 86-87 0.5278040391740717 1102 88-89 0.6337429690401102 1102 90-91 0.6406083071664526 1102 92-93 0.3337759795456634 1102 94-95 0.5123672578466945 1102 96-97 0.5312029918063992 1102 98-99 0.2258933044405218 1102 100-101 0.4434055517978983 1103 1 0.14239581325659145 1103 2 0.2557074821546621 1103 3 0.2622507199580184 1103 4 0.23492978940668507 1103 5 0.2040285384660976 1103 6 0.2172520847037518 1103 7 0.16005411767376643 1103 8 0.006691922251242488 1103 9 0.3532515931907767 1103 10-11 0.41308824541925304 1103 12-13 0.3577595094715207 1103 14-15 0.613990807017359 1103 16-17 0.26708260197153066 1103 18-19 0.4497107265048612 1103 20-21 0.21893250646879636 1103 22-23 0.42542555368763857 1103 24-25 0.2669571438075593 1103 26-27 0.16061692078584144 1103 28-29 0.21397700776974915 1103 30-31 0.39993399962432363 1103 32-33 0.397139102715272 1103 34-35 0.3111515896729671 1103 36-37 0.5620641203932664 1103 38-39 0.4290559658116848 1103 40-41 0.22408090556956495 1103 42-43 0.3989471355711771 1103 44-45 0.5231442796877985 1103 46-47 0.5778647246780793 1103 48-49 0.3600264205399739 1103 50-51 0.44962261608238663 1103 52-53 0.5776374001955134 1103 54-55 0.5918014930244411 1103 56-57 0.27833145901180245 1103 58-59 0.2946284804589183 1103 60-61 0.26267346588523566 1103 62-63 0.1485593690266782 1103 64-65 0.08084053782329548 1103 66-67 0.2622966611661859 1103 68-69 0.151411490787833 1103 70-71 0.09211597183360709 1103 72-73 0.1470105335206533 1103 74-75 -0.24622131752990484 1103 76-77 -0.2986144275845497 1103 78-79 -0.08245716356066524 1103 80-81 0.027473167259564946 1103 82-83 -0.3346954048366655 1103 84-85 -0.3282557026828421 1103 86-87 -0.2071983044713015 1103 88-89 -0.37683691578645906 1103 90-91 -0.4540498554155761 1103 92-93 -0.7148613580731933 1103 94-95 -0.7688685128778268 1103 96-97 -0.6181006107113447 1103 98-99 -0.25939529874683487 1103 100-101 0.22667359786727204 1104 1 0.40779053616601857 1104 2 0.3620017426849351 1104 3 0.2902710248523057 1104 4 0.4182385758940512 1104 5 0.28788409391469827 1104 6 0.42014219505225014 1104 7 0.4587908375092198 1104 8 0.48729510082714 1104 9 0.6126539978190664 1104 10-11 0.5367538114887722 1104 12-13 0.5320092263003886 1104 14-15 0.6365370048232251 1104 16-17 0.6787380220113803 1104 18-19 0.6771797702046669 1104 20-21 0.7902234459551991 1104 22-23 0.685221830909768 1104 24-25 0.5440645627307674 1104 26-27 0.5932590919463507 1104 28-29 0.7341736071491809 1104 30-31 0.9053840411774772 1104 32-33 0.908139458885195 1104 34-35 1.01997305227993 1104 36-37 1.1328697382518271 1104 38-39 1.140284784129726 1104 40-41 1.3518360082652237 1104 42-43 1.2686595363810653 1104 44-45 1.5154208472365553 1104 46-47 1.4457794300036184 1104 48-49 1.3626495638839344 1104 50-51 1.5925670824532077 1104 52-53 1.5935535430356893 1104 54-55 1.6333987707310982 1104 56-57 1.505358499047226 1104 58-59 1.728325524248568 1104 60-61 1.5770993103448703 1104 62-63 1.7680656084177855 1104 64-65 1.8765995487618774 1104 66-67 1.7144527726890821 1104 68-69 1.6563441233327758 1104 70-71 1.7302056175568374 1104 72-73 1.501657107069743 1104 74-75 1.4554823619299988 1104 76-77 1.756025568051566 1104 78-79 1.4474402530843413 1104 80-81 1.5782634794376875 1104 82-83 1.6472201475957178 1104 84-85 1.5056993236448513 1104 86-87 1.326087539539106 1104 88-89 1.4709036513259015 1104 90-91 1.13400032282982 1104 92-93 1.2061178525130742 1104 94-95 1.0989812155024978 1104 96-97 1.27236911644966 1104 98-99 1.2298987467979927 1104 100-101 1.3712553228206659 1105 1 -0.10967299282763321 1105 2 0.08419079803170604 1105 3 0.1201109249671859 1105 4 0.28850015083049385 1105 5 0.15060128715530396 1105 6 -0.061131683149632465 1105 7 0.09342142000095777 1105 8 0.17492808224520218 1105 9 -0.01753412850879954 1105 10-11 0.12658573077012392 1105 12-13 0.2061862418115794 1105 14-15 0.19084408298083844 1105 16-17 0.2333872472314411 1105 18-19 0.1088680438350238 1105 20-21 -0.027266426024119994 1105 22-23 -0.04306741819043225 1105 24-25 -0.117262843170991 1105 26-27 -0.03751824435693152 1105 28-29 -0.003246058435188104 1105 30-31 0.1408669517500556 1105 32-33 0.23726544850075726 1105 34-35 0.052312574396353284 1105 36-37 0.1328611650908016 1105 38-39 0.07184290158826911 1105 40-41 0.12609844363527856 1105 42-43 -0.12123925355622589 1105 44-45 -0.15844987261280608 1105 46-47 -0.06977851910921373 1105 48-49 -0.22246633745924527 1105 50-51 -0.1507368891025962 1105 52-53 0.1051483536233917 1105 54-55 -0.26362977166007084 1105 56-57 -0.15125600642946324 1105 58-59 -0.011887433071116504 1105 60-61 0.17796800536947188 1105 62-63 -0.13915486366721552 1105 64-65 -0.08791302027154657 1105 66-67 -0.1598514373564761 1105 68-69 -0.03877634516708994 1105 70-71 -0.18730888277595525 1105 72-73 -0.008225409449178045 1105 74-75 -0.0987070301081836 1105 76-77 -0.2249646164584469 1105 78-79 -0.2385053743697263 1105 80-81 -0.39114514391528843 1105 82-83 -0.45191988641635206 1105 84-85 -0.5112848877100653 1105 86-87 -0.2926476230469923 1105 88-89 -0.3930932810412102 1105 90-91 -0.3221502966979699 1105 92-93 -0.3584707838399339 1105 94-95 -0.39164598137001505 1105 96-97 -0.2930472196307221 1105 98-99 -0.4789372205741138 1105 100-101 -0.3298289482346277 1106 1 0.1125884704721507 1106 2 0.12883054230062285 1106 3 0.06536660322657539 1106 4 0.07079367107209578 1106 5 0.09072242134318742 1106 6 -0.015535060297132475 1106 7 -0.009553204605310839 1106 8 -0.08995824691800891 1106 9 -0.047707498608005494 1106 10-11 -0.062107254585406224 1106 12-13 0.18628573303591622 1106 14-15 0.27743253916137434 1106 16-17 0.21433721334827993 1106 18-19 0.19794679916841318 1106 20-21 0.08821780132660706 1106 22-23 0.1209791519129908 1106 24-25 0.14950102062624637 1106 26-27 -0.05553291750139522 1106 28-29 0.13688382953153422 1106 30-31 0.16203449454923913 1106 32-33 0.08593525143508174 1106 34-35 0.20939838104953168 1106 36-37 0.0967601283717201 1106 38-39 0.14641981485492295 1106 40-41 0.25185402303733895 1106 42-43 0.3370255965504043 1106 44-45 0.5291420986627173 1106 46-47 0.4270640834194168 1106 48-49 0.4221483528909218 1106 50-51 0.31229726119978807 1106 52-53 0.11330974311976405 1106 54-55 0.20466721085199424 1106 56-57 0.48443133877580635 1106 58-59 0.4043298477938748 1106 60-61 0.22267868543244163 1106 62-63 0.20278207592113517 1106 64-65 0.34923711156337944 1106 66-67 0.4143066528708381 1106 68-69 0.30666738841014407 1106 70-71 0.2588991462503678 1106 72-73 0.15062926423862422 1106 74-75 0.1008715810897094 1106 76-77 0.18008329538798407 1106 78-79 0.21963345150705038 1106 80-81 0.563341243203979 1106 82-83 0.5604289788373329 1106 84-85 0.5333820234586995 1106 86-87 0.7308894771279313 1106 88-89 0.8810599795150154 1106 90-91 0.6215100808313814 1106 92-93 0.7184379549971105 1106 94-95 0.9295022427448387 1106 96-97 1.1592763804061015 1106 98-99 0.6993236752393166 1106 100-101 1.0422255183810272 1107 1 -0.010117685160835777 1107 2 -0.017131219514798346 1107 3 0.09020951099630992 1107 4 0.044924824471543445 1107 5 0.10505876250879709 1107 6 -0.05481705201815146 1107 7 0.07077527450365295 1107 8 0.21000601646088768 1107 9 0.20394978719561863 1107 10-11 0.09336193746914745 1107 12-13 0.17084676820670097 1107 14-15 0.16109718075285429 1107 16-17 0.05550654537126576 1107 18-19 0.10136932515191432 1107 20-21 0.12418447692743229 1107 22-23 0.1460052098658764 1107 24-25 -0.0028389835430289168 1107 26-27 -0.07511509683968143 1107 28-29 0.1002210343321579 1107 30-31 0.11168382895467488 1107 32-33 0.08478572345628521 1107 34-35 0.021908431974210885 1107 36-37 0.26033259926126107 1107 38-39 0.4065213664198879 1107 40-41 0.3138950308100519 1107 42-43 0.18565197017677804 1107 44-45 0.393137452901982 1107 46-47 0.3935818474981616 1107 48-49 0.3147694851461935 1107 50-51 0.5241990450527894 1107 52-53 0.5241759094737333 1107 54-55 0.5472101692486078 1107 56-57 0.3969034195405712 1107 58-59 0.48118591855238435 1107 60-61 0.6355044098435343 1107 62-63 0.6563341471561372 1107 64-65 0.5762681428627054 1107 66-67 0.3210132254310629 1107 68-69 0.46282153236916557 1107 70-71 0.5217628408213812 1107 72-73 0.44648456070367715 1107 74-75 0.4931226904139905 1107 76-77 0.2454105832762501 1107 78-79 0.23611324542586587 1107 80-81 0.3593387416404994 1107 82-83 0.2807936412751708 1107 84-85 0.42681318579230165 1107 86-87 0.2722360032305531 1107 88-89 0.19786363656257677 1107 90-91 0.34717120832177883 1107 92-93 0.5409646830116444 1107 94-95 0.23478381753860944 1107 96-97 0.3583531843609542 1107 98-99 0.7608740489848138 1107 100-101 1.1097245505142048 1108 1 0.17700338372427282 1108 2 0.42080600869469365 1108 3 0.2724682870724422 1108 4 0.2511043164990241 1108 5 0.2961345802437165 1108 6 0.2499759145161633 1108 7 0.1388694321894164 1108 8 0.2954664059485026 1108 9 0.40299812651622346 1108 10-11 0.39937441619859015 1108 12-13 0.5166446506041353 1108 14-15 0.5684851557953934 1108 16-17 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-1.1184102039084287 2316 96-97 -1.3710917008538992 2316 98-99 -1.3981001787427765 2316 100-101 -1.5408871250673997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6820.0 3 1310.0 4 1163.0 5 1151.0 6 1292.0 7 1513.0 8 1887.0 9 2151.0 10 2575.0 11 3493.0 12 4763.0 13 6218.0 14 6560.0 15 7812.0 16 9419.0 17 11639.0 18 10251.0 19 6889.0 20 6755.0 21 6984.0 22 7456.0 23 8354.0 24 9222.0 25 10492.0 26 11946.0 27 13869.0 28 16118.0 29 18852.0 30 22076.0 31 26115.0 32 31552.0 33 38604.0 34 50411.0 35 69687.0 36 95873.0 37 134770.0 38 124726.0 39 20386.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61371024560276 19.996044646057626 14.49093358713521 21.899311521204407 2 15.747822457764201 22.03571490310647 39.349737855278335 22.866724783850998 3 18.16232533494067 26.845029958462046 31.604170346371944 23.388474360225338 4 12.573127575979829 17.802382063648466 37.94756299034679 31.676927370024917 5 12.733134148360678 38.076209257900054 34.25586125259175 14.934795341147513 6 29.270314067062237 37.258224683685015 18.216951815383702 15.254509433869048 7 25.7743831687585 34.90782285479149 22.22619019668659 17.09160377976341 8 25.568385436876405 33.67889157734219 21.372132739841476 19.380590245939928 9 26.78347440468548 16.346704658811444 21.817375974083696 35.05244496241938 10-11 23.883359747217277 27.10144784318182 28.288157974378326 20.727034435222574 12-13 25.415935762816556 25.552501544163064 28.526559604694256 20.505003088326127 14-15 22.492402529485346 26.406473625262255 27.93319165383442 23.167932191417975 16-17 20.558193181347573 29.496849786781898 28.404396761829293 21.540560270041233 18-19 20.40434816989394 27.424099773646077 32.03365161264814 20.13790044381184 20-21 21.48190875915339 26.742810841589936 31.75816092919444 20.017119470062234 22-23 21.21419947827656 26.982938333574303 31.506559850526607 20.29630233762254 24-25 20.80410367424151 27.03850808730996 31.853393605894304 20.303994632554225 26-27 20.585826950859904 27.19688198856102 31.706668447822047 20.510622612757032 28-29 20.36078458528816 27.462649100635357 31.747893485943795 20.42867282813269 30-31 20.66718919600537 27.13027546526952 31.663918030476484 20.53861730824863 32-33 20.404634486438287 27.389958118688007 31.42864553292765 20.776761861946056 34-35 20.949583824067936 27.297689506032324 31.264907609932795 20.487819059966945 36-37 21.07996623829231 27.046370368903588 31.045919882774598 20.827743510029503 38-39 20.764389373829538 27.218936009488047 31.108195074207366 20.908479542475046 40-41 20.799558679923585 27.130300123626515 31.250730534389387 20.819410662060513 42-43 20.71969408732567 27.23376323690353 30.778101334501358 21.268441341269444 44-45 20.53466909863066 27.318883441443536 30.684385850721295 21.462061609204504 46-47 20.855138839561384 27.54887753978203 30.17333443881939 21.422649181837194 48-49 20.934091165079668 27.66434506429481 29.742276144019385 21.659287626606133 50-51 20.72959952473924 27.93945375954157 29.461278315299623 21.869668400419567 52-53 20.872419885331674 27.973130606170304 29.431516152886978 21.722933355611044 54-55 20.467583674545327 27.727122810947414 29.544823096767246 22.26047041774001 56-57 20.679641681720323 28.02176387617233 29.194402514166935 22.104191927940413 58-59 20.869854558318924 27.421286091381607 29.381990366560533 22.326868983738933 60-61 20.59740067023631 27.44592973648089 29.777906933607035 22.178762659675762 62-63 20.69520023511578 27.22083868273291 29.817878080094047 22.266083002057265 64-65 20.51957938516955 27.291007779765188 29.707933875090514 22.481478959974748 66-67 20.48670104286007 27.405389647405727 29.309387597460596 22.798521712273615 68-69 20.513387109552955 27.221735298195576 29.485684433616576 22.779193158634897 70-71 20.458791008136945 27.3213443476517 29.310581742690307 22.90928290152105 72-73 20.580513310573444 27.186905059577597 29.19364142530705 23.03894020454191 74-75 20.453949062246252 27.393896183629412 29.106757372309033 23.045397381815302 76-77 20.486151934546093 27.178011903185446 29.182172696434115 23.15366346583434 78-79 20.59355900329102 27.156608022171085 29.098804840026727 23.151028134511172 80-81 20.71811941434321 27.17209932531298 28.973739486049972 23.136041774293837 82-83 20.80266068528115 26.925083146415034 29.045711191894192 23.22654497640962 84-85 20.766544830825147 26.658474149949047 29.051010791970093 23.52397022725571 86-87 20.988680202574567 26.46060654809599 28.894842515909282 23.655870733420162 88-89 21.20243060282415 26.185908939024543 28.967550709750878 23.644109748400428 90-91 21.146558313553527 26.216295672786806 29.07535719701307 23.561788816646605 92-93 21.51912836166752 25.993738998685412 29.001817151033972 23.485315488613097 94-95 21.455832689603263 25.67158377404084 29.01192386158813 23.860659674767764 96-97 21.680628784286828 25.322581244173854 28.91470584230132 24.082084129237998 98-99 21.760977170752962 25.280963206926454 28.713303949051838 24.24475567326874 100-101 22.224463617310526 24.562871235358656 29.29123168304088 23.921433464289933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1730.0 1 1204.5 2 762.5 3 854.5 4 750.5 5 756.5 6 1114.5 7 1782.5 8 2562.0 9 2768.5 10 2278.5 11 1934.5 12 1892.0 13 1909.5 14 2021.0 15 2129.0 16 2185.0 17 2295.5 18 2433.5 19 2592.0 20 2890.0 21 3149.0 22 3525.5 23 4069.5 24 4572.0 25 5317.0 26 6431.0 27 7631.0 28 8513.5 29 9516.5 30 11003.0 31 12655.5 32 14369.5 33 15811.5 34 17479.5 35 18853.0 36 20308.0 37 22073.0 38 22356.5 39 22109.0 40 22721.5 41 24012.0 42 25061.0 43 26008.0 44 27103.0 45 28100.5 46 28549.0 47 28877.5 48 29984.5 49 29833.0 50 28043.5 51 26216.5 52 24771.5 53 24023.0 54 22988.5 55 20967.5 56 19006.0 57 17002.0 58 15314.0 59 13802.0 60 12017.0 61 10270.5 62 8630.5 63 6975.5 64 5304.5 65 4069.0 66 3116.0 67 2470.5 68 1892.5 69 1331.5 70 1010.0 71 815.0 72 684.5 73 577.0 74 456.5 75 378.0 76 289.0 77 200.5 78 142.0 79 87.0 80 68.5 81 56.0 82 40.5 83 41.0 84 28.0 85 21.5 86 14.0 87 10.5 88 11.0 89 6.0 90 6.0 91 5.0 92 5.0 93 4.0 94 3.0 95 3.0 96 2.5 97 2.5 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2623409989175968 2 0.16026470798537398 3 0.1894821970565537 4 0.15681285273645826 5 0.22930181296368893 6 0.18763298888749172 7 0.23435631529245843 8 0.23867113435360313 9 0.14374511500842008 10-11 0.19835839626805135 12-13 0.20439914295365388 14-15 0.16630545467097654 16-17 0.2767031823639784 18-19 0.22264466355506574 20-21 0.19151632604252192 22-23 0.23632880400612458 24-25 0.22615815907628353 26-27 0.33211778716353657 28-29 0.3052426284398354 30-31 0.2800933973405921 32-33 0.18356473091555528 34-35 0.3508564299433649 36-37 0.38734747114618856 38-39 0.3678075048264333 40-41 0.329713816543756 42-43 0.3641090884883093 44-45 0.37378661123973383 46-47 0.3781014303008785 48-49 0.3993056839727895 50-51 0.3920937721134477 52-53 0.391477369390427 54-55 0.38605302542784514 56-57 0.36219824004694523 58-59 0.3358162035016606 60-61 0.306721994975085 62-63 0.3754508985918896 64-65 0.40596283338141276 66-67 0.41070913434867196 68-69 0.4149623131375145 70-71 0.41434591041449387 72-73 0.4073189193720583 74-75 0.4031273808555177 76-77 0.38697762951237613 78-79 0.3572670182627799 80-81 0.37051967680772424 82-83 0.38161492582209633 84-85 0.3826011701789294 86-87 0.3773001067609516 88-89 0.38562154352173067 90-91 0.40750384018896446 92-93 0.4068257971936417 94-95 0.4149623131375145 96-97 0.41631839912816004 98-99 0.41379114796377525 100-101 0.38580646433863686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811158.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.609004564388435 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31533544146988 52.16290216938542 2 9.925650798172061 12.428702322545607 3 2.7910930504905074 5.2424267261337905 4 1.1750034962505338 2.942631970396882 5 0.6277170558358944 1.965037000698699 6 0.4141903105341479 1.5559225825654759 7 0.28035079277333574 1.2286738845062988 8 0.2038081934100649 1.0208182489176412 9 0.15814413964499816 0.8911122444778475 >10 0.9972786358522853 11.656120555670313 >50 0.08317674005741563 3.5600762353387307 >100 0.026275726947254153 2.7081019588235 >500 9.878092807840408E-4 0.4760886279124814 >1k 7.902474246272327E-4 1.3544220513488365 >5k 1.9756185615680817E-4 0.8069634212785214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6524 0.8042822729973692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4735 0.5837333787005737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3419 0.42149618200153355 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1674 0.20637163166732003 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.13832077104583818 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 0.11206201504515767 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2328054460413384E-4 0.0 8 0.0 0.0 0.0 2.465610892082677E-4 0.0 9 0.0 0.0 0.0 3.6984163381240153E-4 0.0 10-11 0.0 0.0 0.0 4.931221784165354E-4 0.0 12-13 0.0 0.0 0.0 4.931221784165354E-4 0.0 14-15 0.0 0.0 0.0 4.931221784165354E-4 0.0 16-17 0.0 0.0 0.0 6.164027230206692E-4 0.0 18-19 0.0 0.0 0.0 6.780429953227361E-4 0.0 20-21 0.0 0.0 0.0 9.246040845310038E-4 0.0 22-23 0.0 0.0 0.0 0.0027121719812909445 0.0 24-25 0.0 0.0 0.0 0.00560926477948809 0.0 26-27 0.0 0.0 0.0 0.011095249014372047 0.0 28-29 0.0 0.0 0.0 0.031066697240241727 0.0 30-31 0.0 0.0 0.0 0.08641966176749782 0.0 32-33 0.0 0.0 0.0 0.18627690289684623 0.0 34-35 0.0 0.0 0.0 0.3420418710041694 0.0 36-37 0.0 0.0 0.0 0.5813294080807931 0.0 38-39 0.0 0.0 0.0 0.9473493450104664 0.0 40-41 0.0 0.0 0.0 1.370571454636458 0.0 42-43 0.0 0.0 0.0 1.7465154754067642 0.0 44-45 0.0 0.0 0.0 2.182497121399283 0.0 46-47 0.0 0.0 0.0 2.6973783159384483 0.0 48-49 0.0 0.0 0.0 3.233833605783337 0.0 50-51 0.0 0.0 0.0 3.8305730819396464 0.0 52-53 0.0 0.0 0.0 4.3788016637942295 1.2328054460413384E-4 54-55 0.0 0.0 0.0 4.94601544951785 1.2328054460413384E-4 56-57 0.0 0.0 0.0 5.581711577769066 1.2328054460413384E-4 58-59 0.0 0.0 0.0 6.276582367430266 1.2328054460413384E-4 60-61 0.0 0.0 0.0 7.057996099403569 1.2328054460413384E-4 62-63 0.0 0.0 0.0 7.8007613806434755 1.2328054460413384E-4 64-65 0.0 0.0 0.0 8.536684591657853 1.2328054460413384E-4 66-67 0.0 0.0 0.0 9.334556276335807 1.2328054460413384E-4 68-69 0.0 0.0 0.0 10.226059534640601 1.2328054460413384E-4 70-71 0.0 0.0 0.0 11.21698855216863 1.2328054460413384E-4 72-73 0.0 0.0 0.0 12.207917569696656 1.2328054460413384E-4 74-75 0.0 0.0 0.0 13.179183340360325 1.2328054460413384E-4 76-77 0.0 0.0 0.0 14.186323749503796 1.2328054460413384E-4 78-79 0.0 0.0 0.0 15.27310339046154 1.2328054460413384E-4 80-81 0.0 0.0 0.0 16.431324107017375 1.2328054460413384E-4 82-83 0.0 0.0 0.0 17.60162631694442 1.2328054460413384E-4 84-85 0.0 0.0 0.0 18.75047771211034 1.2328054460413384E-4 86-87 0.0 0.0 0.0 19.902780962525178 1.2328054460413384E-4 88-89 0.0 0.0 0.0 21.097184518922333 1.2328054460413384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3520 0.0 39.999447 1 GTATCAA 5750 0.0 37.309002 1 TATCAAC 7145 0.0 29.81398 2 ATCAACG 7135 0.0 29.78927 3 TCAACGC 7200 0.0 29.454447 4 CAACGCA 7220 0.0 29.241434 5 AACGCAG 7330 0.0 28.867338 6 GTACATG 4080 0.0 27.164434 1 TACATGG 4105 0.0 26.004303 2 ACATGGG 4110 0.0 25.741798 3 ACGCAGA 8160 0.0 25.582228 7 CGCAGAG 8220 0.0 25.280062 8 TAAGGTG 245 1.0913936E-11 25.17405 5 GAGTACT 4730 0.0 24.03524 12-13 CATGGGA 2520 0.0 23.90997 4 GCAGAGT 8600 0.0 23.776869 9 CATGGGG 1430 0.0 23.224043 4 AGTACTT 4895 0.0 22.400791 12-13 AGAGTAC 7190 0.0 21.322466 10-11 GTACTTT 5325 0.0 21.30504 14-15 >>END_MODULE